FastQCFastQC Report
Thu 26 May 2016
SRR936886_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936886_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308944
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80970.6185902529061595No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT72010.5501381266119865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63630.48611705313596304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42070.3214041242406092No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33020.2522644207849992No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT27880.212996125120708No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT22140.1691439817135034No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC20950.16005268369005854No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC20550.15699678519478297No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC19290.1473707049346649No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT17250.13178562260875942No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15850.1210899778752949No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG14730.11253346208852327No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC14180.10833160165751934No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA13840.1057340879365351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG69800.024.9161361
TTTGCGT2351.7644197E-1024.2541393
TACATGG71500.023.8503112
GAGTACT67700.022.20364812-13
ACATGGG72550.021.9320833
GTACTTT69650.021.71963514-15
AGTACTT70050.021.22147212-13
TACACCG1404.856327E-420.3545975
TTGCGTT3052.0918378E-1020.243374
TAGTACG1200.004753614319.7990251
CGCACTA600.00476959919.78843794-95
CATGGGG23750.019.3975754
CATGGGA50700.019.3910664
AGAGTAC119100.019.29995510-11
CTGGTCG3054.5729394E-918.6854749
GTATCAA174050.018.5374831
TACTTTT79600.018.52732514-15
CTACACG2603.2852586E-718.2669454
ACTTTTT83650.018.19845616-17
CTTTGCG3156.8048394E-918.0957412
Produced by FastQC (version 0.11.5)