##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936886_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1308944 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.097891888423035 31.0 31.0 34.0 31.0 34.0 2 32.15616176093095 33.0 31.0 34.0 30.0 34.0 3 32.295092074221664 34.0 31.0 34.0 30.0 34.0 4 35.99505250033615 37.0 35.0 37.0 35.0 37.0 5 35.90573011526849 37.0 35.0 37.0 35.0 37.0 6 35.946554627241504 37.0 35.0 37.0 35.0 37.0 7 35.89787110831327 37.0 35.0 37.0 35.0 37.0 8 35.88914957400775 37.0 35.0 37.0 35.0 37.0 9 37.556107060347884 39.0 37.0 39.0 35.0 39.0 10-11 37.45013270239215 39.0 37.0 39.0 34.5 39.0 12-13 37.377626544756694 39.0 37.0 39.0 34.0 39.0 14-15 38.533910159640136 40.0 38.0 41.0 34.0 41.0 16-17 38.46972597758193 40.0 38.0 41.0 34.0 41.0 18-19 38.54782748536225 40.0 38.0 41.0 34.0 41.0 20-21 38.652155859990955 40.0 38.0 41.0 34.0 41.0 22-23 38.589197093229345 40.0 38.0 41.0 34.0 41.0 24-25 38.495429903800314 40.0 38.0 41.0 34.0 41.0 26-27 38.31646273637375 40.0 38.0 41.0 34.0 41.0 28-29 38.16749608844992 40.0 38.0 41.0 33.5 41.0 30-31 38.099076431077265 40.0 37.0 41.0 34.0 41.0 32-33 37.98598794142454 40.0 37.0 41.0 33.0 41.0 34-35 37.85447009192143 40.0 37.0 41.0 33.0 41.0 36-37 37.669735680059645 40.0 37.0 41.0 33.0 41.0 38-39 37.523137353469664 40.0 37.0 41.0 33.0 41.0 40-41 37.30327500641739 40.0 36.0 41.0 32.0 41.0 42-43 37.055139486486816 40.0 36.0 41.0 31.5 41.0 44-45 36.78067434512096 39.0 36.0 41.0 31.0 41.0 46-47 36.384285347577894 39.0 36.0 41.0 30.0 41.0 48-49 35.97650319646982 39.0 35.0 41.0 29.0 41.0 50-51 35.71403933246953 39.0 35.0 40.0 28.0 41.0 52-53 35.50841136060824 39.0 35.0 40.0 28.0 41.0 54-55 35.258221512914226 38.5 35.0 40.0 27.0 41.0 56-57 35.00349403794203 38.0 34.5 40.0 26.5 41.0 58-59 34.66234002371377 38.0 34.0 40.0 26.0 41.0 60-61 34.31056561625249 38.0 34.0 40.0 26.0 41.0 62-63 33.98713772323339 37.0 33.5 40.0 25.0 41.0 64-65 33.57949767140535 36.5 33.0 39.0 23.5 41.0 66-67 33.13995556723588 36.0 32.5 39.0 22.5 40.0 68-69 32.69404458861494 35.5 32.0 39.0 22.0 40.0 70-71 32.28072973328118 35.0 31.5 38.0 21.0 40.0 72-73 31.879121643095502 35.0 31.0 37.5 20.0 39.5 74-75 31.403093638841693 35.0 31.0 37.0 18.5 39.0 76-77 29.93503961972399 33.5 29.5 35.5 17.0 37.5 78-79 30.643327751225414 34.0 30.0 36.0 17.5 38.0 80-81 30.36987907809654 34.0 30.0 35.5 11.0 37.0 82-83 30.133008745981492 34.0 30.0 35.0 9.5 37.0 84-85 29.787168129423414 34.0 30.0 35.0 7.0 36.5 86-87 29.445025150044614 34.0 29.5 35.0 4.5 36.0 88-89 29.051044200515832 34.0 29.0 35.0 2.0 36.0 90-91 28.58476795034776 34.0 29.0 35.0 2.0 35.0 92-93 28.144088287963427 33.5 28.5 35.0 2.0 35.0 94-95 27.647309204977446 33.0 27.0 35.0 2.0 35.0 96-97 26.993252958109743 33.0 26.0 35.0 2.0 35.0 98-99 26.208958901221138 33.0 25.0 34.0 2.0 35.0 100-101 22.960988781796623 29.0 10.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.38196353374986813 1101 2 0.2708686383326864 1101 3 0.2927113850118914 1101 4 0.29917711312650397 1101 5 0.35135584741812664 1101 6 0.3485069510982939 1101 7 0.3248803314167503 1101 8 0.30258285411472485 1101 9 0.40451304129792476 1101 10-11 0.41278283698496665 1101 12-13 0.43728180667907424 1101 14-15 0.5438586722402405 1101 16-17 0.5809922069409836 1101 18-19 0.5187327089593055 1101 20-21 0.46328393971334947 1101 22-23 0.5044608907319983 1101 24-25 0.4258392739744181 1101 26-27 0.35954996715648235 1101 28-29 0.35387965488973094 1101 30-31 0.3191339749257125 1101 32-33 0.46044015091529644 1101 34-35 0.5884067593680484 1101 36-37 0.6642664821106621 1101 38-39 0.6278979179920512 1101 40-41 0.6394724849450952 1101 42-43 0.5776950923778514 1101 44-45 0.8163321260329113 1101 46-47 0.9142611879467495 1101 48-49 0.7842651081237477 1101 50-51 0.736004163915652 1101 52-53 0.7742340679018511 1101 54-55 0.8831824494953722 1101 56-57 0.9612512202036427 1101 58-59 0.9655118271534207 1101 60-61 1.0552070906567153 1101 62-63 1.0262101059486852 1101 64-65 1.0686847317874921 1101 66-67 0.8915956524564734 1101 68-69 0.9375157817137065 1101 70-71 0.8500966798815313 1101 72-73 0.9540766012995299 1101 74-75 0.971175561315615 1101 76-77 0.9164679966011633 1101 78-79 0.995400174308287 1101 80-81 1.3490535535837687 1101 82-83 1.2119616023538562 1101 84-85 0.9088210535767693 1101 86-87 0.9327814625656146 1101 88-89 0.8028605876345587 1101 90-91 0.7284720443318413 1101 92-93 0.8604507248493363 1101 94-95 0.9313573853883277 1101 96-97 0.8822123914752993 1101 98-99 1.0071886696216268 1101 100-101 1.2144215726301155 1102 1 0.31516180916413106 1102 2 0.32724932584942223 1102 3 0.27393114182772393 1102 4 0.23503658895793222 1102 5 0.22344041445360574 1102 6 0.30116475508849305 1102 7 0.1612684511148288 1102 8 0.2837526634752763 1102 9 0.283792039791507 1102 10-11 0.30080363910114016 1102 12-13 0.3284473005794908 1102 14-15 0.315943439065542 1102 16-17 0.3209337883972907 1102 18-19 0.4158179752591593 1102 20-21 0.2957471883757563 1102 22-23 0.31038405955839465 1102 24-25 0.30904214455456724 1102 26-27 0.400425965606118 1102 28-29 0.4177534596471233 1102 30-31 0.5401065118168944 1102 32-33 0.4161287664917097 1102 34-35 0.4364865793973749 1102 36-37 0.469595276219259 1102 38-39 0.4189976810413256 1102 40-41 0.307479741311397 1102 42-43 0.36067040235176506 1102 44-45 0.5208499732576328 1102 46-47 0.6993332920931934 1102 48-49 0.634207968234918 1102 50-51 0.5476652964042685 1102 52-53 0.5624029142754736 1102 54-55 0.5481878637995194 1102 56-57 0.6334121069507788 1102 58-59 0.5269641792789841 1102 60-61 0.5922181550170151 1102 62-63 0.5670751159581542 1102 64-65 0.7582293308692627 1102 66-67 0.5527571106024993 1102 68-69 0.3974559246832996 1102 70-71 0.40537452758574943 1102 72-73 0.5944039769003986 1102 74-75 0.5534869697139762 1102 76-77 0.6646049565193302 1102 78-79 0.8270362931113056 1102 80-81 0.6458173580614535 1102 82-83 0.73364179479141 1102 84-85 0.6508963903225116 1102 86-87 0.580075509228525 1102 88-89 0.5702762663405601 1102 90-91 0.7294270396767395 1102 92-93 0.7672647564880535 1102 94-95 0.8214879680154041 1102 96-97 0.9196979566607233 1102 98-99 1.2508513364960727 1102 100-101 1.2254509697043972 1103 1 0.37022632396971744 1103 2 0.2478558573324392 1103 3 0.27159389042511606 1103 4 0.1679973467248317 1103 5 0.3054685794227865 1103 6 0.3580959939984112 1103 7 0.25062632879311764 1103 8 0.23005357529820714 1103 9 0.41049722046373205 1103 10-11 0.3552969824860668 1103 12-13 0.4040810380278259 1103 14-15 0.5969052891737476 1103 16-17 0.5345448482247761 1103 18-19 0.5762132951151528 1103 20-21 0.5565866244609694 1103 22-23 0.5495766757414557 1103 24-25 0.5645814175578323 1103 26-27 0.6050405947741879 1103 28-29 0.5497773937168873 1103 30-31 0.5700882035612622 1103 32-33 0.4574935007912302 1103 34-35 0.43481216890270247 1103 36-37 0.6189992322384157 1103 38-39 0.5912292625258786 1103 40-41 0.7392904636925977 1103 42-43 0.713067142764281 1103 44-45 0.6752040815891647 1103 46-47 0.6229636345652665 1103 48-49 0.5226135089182193 1103 50-51 0.48142992505951554 1103 52-53 0.4373195783779522 1103 54-55 0.301804000706305 1103 56-57 0.29834154067694385 1103 58-59 0.46475713701101284 1103 60-61 0.5202351709251971 1103 62-63 0.5255334531377613 1103 64-65 0.3735963421619104 1103 66-67 0.566034442212711 1103 68-69 0.48490156403630635 1103 70-71 0.5830581999038742 1103 72-73 0.45785169836875284 1103 74-75 0.44810680417041837 1103 76-77 0.48683923039442334 1103 78-79 0.6181305062665814 1103 80-81 1.0076086183053619 1103 82-83 0.8867362489797408 1103 84-85 0.7810683443313557 1103 86-87 0.8291972770777924 1103 88-89 0.8085694958758296 1103 90-91 0.8947827685709626 1103 92-93 0.9221150339324851 1103 94-95 0.9794171863503287 1103 96-97 1.3534337382745179 1103 98-99 1.59904025512947 1103 100-101 1.7304254817406601 1104 1 0.24090457232704665 1104 2 0.16017435885687803 1104 3 0.1257448347706145 1104 4 0.058505844324983514 1104 5 -0.11967304724629457 1104 6 0.1526731767597056 1104 7 0.10098882262996511 1104 8 0.14141993305270262 1104 9 0.2156020694791394 1104 10-11 0.17434501480612852 1104 12-13 0.22445854982585445 1104 14-15 0.2833500069769528 1104 16-17 0.3310382674900296 1104 18-19 0.24976948291764955 1104 20-21 0.30131838908398834 1104 22-23 0.32297840746079487 1104 24-25 0.21837276097357972 1104 26-27 0.3679222069535797 1104 28-29 0.28859127665226225 1104 30-31 0.5672317722514535 1104 32-33 0.4169129320708507 1104 34-35 0.3393034700568407 1104 36-37 0.3580873124549626 1104 38-39 0.41142313761266536 1104 40-41 0.38897953906918303 1104 42-43 0.4490616990678049 1104 44-45 0.49827325477116347 1104 46-47 0.2708697987213924 1104 48-49 0.4510719164609043 1104 50-51 0.49764278264798634 1104 52-53 0.5640666427061447 1104 54-55 0.5129252160291529 1104 56-57 0.41408423797320637 1104 58-59 0.38516948947275154 1104 60-61 0.46364361922014297 1104 62-63 0.43385282504988965 1104 64-65 0.657610011776832 1104 66-67 0.47902837465451853 1104 68-69 0.49432803891581045 1104 70-71 0.36046912352106375 1104 72-73 0.6314028550284796 1104 74-75 0.6179430907845607 1104 76-77 0.5965294629338445 1104 78-79 0.7551983311023136 1104 80-81 0.8798974612431465 1104 82-83 0.7960926651961238 1104 84-85 0.5828476079732994 1104 86-87 0.7696222577078942 1104 88-89 0.9152385271795964 1104 90-91 0.7236993874417728 1104 92-93 0.8515463387090527 1104 94-95 0.9303019128070922 1104 96-97 1.1814070178752445 1104 98-99 1.3608043247202382 1104 100-101 1.2186777633639103 1105 1 0.20095856136037327 1105 2 0.17658031918742267 1105 3 0.05412126021202823 1105 4 0.11326702361580487 1105 5 0.17394065705973105 1105 6 0.1344488187972388 1105 7 0.07630890833694792 1105 8 0.10438690698696718 1105 9 0.1277083637536336 1105 10-11 0.1482496015489545 1105 12-13 0.0856013748032467 1105 14-15 0.25134877839604286 1105 16-17 0.1228580478873127 1105 18-19 0.2408356561940579 1105 20-21 0.2490006051306679 1105 22-23 -0.04554875164165395 1105 24-25 0.20687378213968088 1105 26-27 0.19747159676248316 1105 28-29 0.06909134823790453 1105 30-31 0.0765507541935122 1105 32-33 0.06337072774707764 1105 34-35 0.08918936132253208 1105 36-37 0.02616366557652583 1105 38-39 0.15709220637526755 1105 40-41 0.2708724102407274 1105 42-43 0.3555472968935547 1105 44-45 0.2854050934942265 1105 46-47 0.13235342029400243 1105 48-49 0.05301568073979723 1105 50-51 0.1608173549390841 1105 52-53 0.1724986969203357 1105 54-55 0.18521074361532186 1105 56-57 0.10361333906559622 1105 58-59 0.21154035448426356 1105 60-61 0.11821567781512954 1105 62-63 0.17988135162162422 1105 64-65 0.21167889062333956 1105 66-67 0.11636450778178187 1105 68-69 0.1609423929991891 1105 70-71 0.23697594006480927 1105 72-73 0.16389753505290017 1105 74-75 0.023470542348004386 1105 76-77 -0.057283604321170856 1105 78-79 0.23226492287938783 1105 80-81 0.34425607226146226 1105 82-83 0.3616458386426231 1105 84-85 0.03179175037120885 1105 86-87 0.15241423228459183 1105 88-89 0.00969302624531565 1105 90-91 -0.022583971772522915 1105 92-93 -0.016258233716648363 1105 94-95 0.14798436135415116 1105 96-97 0.3323257614432471 1105 98-99 0.49747293704789186 1105 100-101 0.8684548077457848 1106 1 0.15460098867255567 1106 2 0.09240112122959232 1106 3 0.18872672174747862 1106 4 0.08830269879904762 1106 5 0.020517203607099077 1106 6 0.13196549390574575 1106 7 0.026119886210622667 1106 8 0.13353676410829962 1106 9 0.2185475223531057 1106 10-11 0.3071147807908048 1106 12-13 0.1977178295144455 1106 14-15 0.36275143289064005 1106 16-17 0.17221969045222352 1106 18-19 0.17124443507672282 1106 20-21 0.09115941930299698 1106 22-23 0.1794640890518977 1106 24-25 0.06113517239830912 1106 26-27 0.14641931230522687 1106 28-29 0.2520682658308928 1106 30-31 0.08763704721280163 1106 32-33 0.09082045331803101 1106 34-35 0.04501885826390151 1106 36-37 0.04122553221598224 1106 38-39 0.17991779950855147 1106 40-41 0.3059304691548945 1106 42-43 0.20222911933493748 1106 44-45 0.26328054884952934 1106 46-47 0.35592383296364005 1106 48-49 0.6218797568088945 1106 50-51 0.3946208810587706 1106 52-53 0.43028199583388727 1106 54-55 0.4131342403645206 1106 56-57 0.4198203108801195 1106 58-59 0.3875827044557312 1106 60-61 0.2440295642689918 1106 62-63 0.3162391378267486 1106 64-65 0.2666335004026692 1106 66-67 0.4142362740325254 1106 68-69 0.3793652592792327 1106 70-71 0.4215047133011325 1106 72-73 0.428815574062515 1106 74-75 0.6066548089694344 1106 76-77 0.32630260862912763 1106 78-79 0.29932448115953036 1106 80-81 0.9000958892059572 1106 82-83 0.7502325082827177 1106 84-85 0.5975022944488906 1106 86-87 0.42597379115783696 1106 88-89 0.453833636881086 1106 90-91 0.33930206217910097 1106 92-93 0.5157598725509125 1106 94-95 0.5725261591461468 1106 96-97 0.8573164793714625 1106 98-99 0.8501631957952824 1106 100-101 1.040254687464369 1107 1 0.12776124070672523 1107 2 0.03186610213329999 1107 3 0.05047693854114499 1107 4 0.1185719380839032 1107 5 0.009496474442357794 1107 6 0.05232034362622073 1107 7 0.16600600034556834 1107 8 0.1451790453312043 1107 9 0.06478315400682533 1107 10-11 0.014021850109422473 1107 12-13 0.07434089912827346 1107 14-15 0.044933069083192834 1107 16-17 0.1945575103381998 1107 18-19 0.19164956247759335 1107 20-21 0.25792621850593633 1107 22-23 0.15244675478373892 1107 24-25 0.2917944096536047 1107 26-27 0.1850996846368247 1107 28-29 0.13933786490098754 1107 30-31 0.2778164093781541 1107 32-33 0.2056109706707474 1107 34-35 0.12985667814987778 1107 36-37 0.07587850090543924 1107 38-39 0.19238228224808296 1107 40-41 0.3119153581749856 1107 42-43 0.26490266958494146 1107 44-45 0.44488791245402837 1107 46-47 0.3505195588995491 1107 48-49 0.39044790382267536 1107 50-51 0.39354094877219836 1107 52-53 0.43154009100170043 1107 54-55 0.19887636660300956 1107 56-57 0.2570146087454006 1107 58-59 0.471491079148997 1107 60-61 0.3898561708484536 1107 62-63 0.4434647323949079 1107 64-65 0.3081154696271895 1107 66-67 0.16093074717778677 1107 68-69 0.13936256088996402 1107 70-71 -0.0952881472682563 1107 72-73 0.019488372453626113 1107 74-75 0.044787316026763335 1107 76-77 0.17151423614993178 1107 78-79 0.3287484557716098 1107 80-81 0.4899032426626633 1107 82-83 0.4640446865516843 1107 84-85 0.5481454105580461 1107 86-87 0.44965042725813475 1107 88-89 0.23139295531869308 1107 90-91 0.3329158742420546 1107 92-93 0.24587424274224645 1107 94-95 0.31556512204214116 1107 96-97 0.4311762369315666 1107 98-99 0.5343052002603095 1107 100-101 1.131718552111316 1108 1 0.07984330784769611 1108 2 0.08031583578600987 1108 3 0.06170285109342899 1108 4 0.12729302420881794 1108 5 0.1248235095858945 1108 6 0.14578098142965956 1108 7 0.18070202479946573 1108 8 0.09864871034139355 1108 9 0.2082844589297821 1108 10-11 0.17697075254408645 1108 12-13 0.05044673195513383 1108 14-15 0.15221605860805454 1108 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-1.2158743018594116 2316 96-97 -1.3858954360769857 2316 98-99 -1.6679865167112382 2316 100-101 -1.8433940614389428 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 10.0 5 33.0 6 75.0 7 160.0 8 378.0 9 718.0 10 1310.0 11 2171.0 12 3171.0 13 4607.0 14 5819.0 15 8048.0 16 11003.0 17 15216.0 18 13396.0 19 7916.0 20 6591.0 21 6780.0 22 7299.0 23 8143.0 24 9140.0 25 11138.0 26 13584.0 27 16712.0 28 20784.0 29 26059.0 30 32769.0 31 41101.0 32 51865.0 33 66985.0 34 90833.0 35 127686.0 36 191726.0 37 284470.0 38 206284.0 39 14955.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.20725101939759 20.020540785073695 15.582798926499446 21.189409269029262 2 15.28874253647244 21.795601532625593 39.61394032234035 23.301715608561615 3 19.87292234295992 25.98689186938324 30.343610894204335 23.796574893452505 4 12.732706670415237 17.709084575046756 38.248771528804895 31.30943722573311 5 13.04623532440822 38.550421692868234 33.92566684315195 14.47767613957159 6 29.957507731423195 37.57318876896185 17.626575315674316 14.842728183940643 7 27.028352626239165 34.13675451356208 21.05208473395348 17.78280812624528 8 26.163191340792793 33.98255996063997 19.874677411222194 19.979571287345046 9 26.24759061318942 17.32130428903321 21.0922095156168 35.33889558216057 10-11 24.455362490679512 27.359497426933466 27.1931419525969 20.991998129790122 12-13 26.39483675193009 24.968151795703942 27.72869875590505 20.908312696460925 14-15 22.987194257355547 25.870281692723296 27.511490178342235 23.63103387157892 16-17 21.074859228072103 29.210733694769864 27.762046260973065 21.952360816184964 18-19 20.96946466321893 27.77449676325583 30.627858843879142 20.6281797296461 20-21 22.410252414011236 26.097105936874726 30.6181580095361 20.874483639577942 22-23 21.74122804336931 26.304830458751482 30.66685816963904 21.28708332824017 24-25 21.83132353384978 26.46288406448727 30.74922538646635 20.956567015196597 26-27 21.674318892089676 26.163391025217393 31.242166480693207 20.920123601999727 28-29 21.14019723383651 27.05098550065802 30.87979737976586 20.929019885739606 30-31 21.50299614906386 26.388541802794467 30.69797548820684 21.410486559934828 32-33 21.422961650619317 26.541725779428987 30.769921287393835 21.265391282557857 34-35 22.0211523225458 26.38887084981362 30.587514430199093 21.00246239744149 36-37 21.846156667421845 26.16175628399977 30.933481465577017 21.058605583001366 38-39 21.490859289792315 26.72120595105981 30.78846385260259 20.999470906545287 40-41 21.719599105302667 26.649946803328945 30.500430730675852 21.130023360692533 42-43 21.39377547551104 26.88032669776255 29.88004782806346 21.845849998662953 44-45 21.75483069504021 26.556092522283098 29.880536681499787 21.808540101176906 46-47 22.311175489542197 27.0737023531488 29.330780096980195 21.284342060328804 48-49 22.12383329475194 27.14724570848814 29.085750782514758 21.64317021424517 50-51 21.6443504223355 27.357492329507256 29.3859327441417 21.612224504015547 52-53 22.125546546301056 27.914105039265763 28.37135887282537 21.588989541607805 54-55 21.801521946985883 27.294275298902033 28.703266177228397 22.200936576883688 56-57 22.168866431155877 27.386189309114023 28.44312292196362 22.00182133776648 58-59 22.667146959752408 27.150336574420344 28.42470489096761 21.757811574859637 60-61 22.148519836234275 27.341908700378852 29.260976222799616 21.248595240587253 62-63 21.95667262440682 27.265045840474915 29.13048444105409 21.647797094064174 64-65 21.73938290239189 27.718850675092572 28.849448797600587 21.692317624914953 66-67 22.046968880318705 27.44664441694956 28.33500327175507 22.17138343097666 68-69 21.78154168791506 27.41854464263545 28.656929650151174 22.142984019298314 70-71 22.302053015575538 27.29884607406564 28.49647351035752 21.902627400001297 72-73 22.061950587758876 27.012763746528268 28.948337580274835 21.976948085438018 74-75 21.753630252845603 28.130467535920516 28.140743638366768 21.97515857286712 76-77 21.994667237124588 27.371398666045277 28.297717913652026 22.336216183178113 78-79 21.966202316212918 27.506508737849245 28.40881177093063 22.11847717500721 80-81 22.325851452320197 27.63824163063242 27.987837486821398 22.048069430225983 82-83 22.080781193534264 27.490948291998663 28.41506674846757 22.0132037659995 84-85 21.856223950603983 27.76100200812647 28.15086198170034 22.231912059569197 86-87 21.863043797137234 27.70649470107201 28.114495348922492 22.315966152868267 88-89 21.876696290269287 27.653967289889202 27.74327739037262 22.726059029468896 90-91 21.904254762413178 27.256714783888874 28.11834017548035 22.7206902782176 92-93 22.16192326468496 27.538941785764216 28.169604283157145 22.129530666393673 94-95 21.838004302731377 27.444771936901425 28.116653526222823 22.600570234144374 96-97 21.357472458630646 27.55550292603175 28.47960945497884 22.607415160358762 98-99 21.64316677133135 27.67666558182104 27.725292640926796 22.95487500592081 100-101 21.38973945250188 27.54683957046028 27.949609453785364 23.113811523252473 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 718.0 1 505.0 2 310.5 3 345.5 4 306.5 5 287.0 6 536.5 7 1199.5 8 2053.5 9 2477.5 10 2184.5 11 1814.5 12 1823.0 13 1960.5 14 2127.5 15 2264.5 16 2358.5 17 2490.5 18 2669.5 19 2860.5 20 3104.5 21 3556.5 22 4126.5 23 4783.0 24 5609.0 25 6807.5 26 8402.5 27 10350.0 28 11953.5 29 13730.5 30 16324.0 31 18832.5 32 21300.5 33 24003.0 34 27025.5 35 29892.5 36 33673.0 37 37063.0 38 38516.5 39 39622.5 40 40088.5 41 40985.5 42 41784.0 43 42611.5 44 44455.0 45 45822.0 46 46529.5 47 51806.5 48 64563.0 49 63464.0 50 50027.5 51 44272.5 52 42038.0 53 40202.0 54 38388.0 55 35399.0 56 31792.5 57 28476.5 58 25750.5 59 22791.5 60 19310.0 61 15642.0 62 12177.0 63 8987.5 64 6305.0 65 4614.5 66 3291.5 67 2352.5 68 1745.5 69 1171.5 70 755.0 71 531.5 72 373.0 73 298.5 74 213.0 75 138.0 76 86.5 77 36.0 78 22.0 79 14.0 80 6.0 81 4.0 82 2.5 83 1.5 84 1.0 85 0.5 86 1.0 87 1.0 88 1.0 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025058367661259764 2 0.0060353995281692725 3 0.010542849808700754 4 0.0 5 0.0040490655062401446 6 0.0 7 0.0 8 1.5279492476377905E-4 9 7.639746238188952E-5 10-11 0.0 12-13 3.819873119094476E-5 14-15 0.0 16-17 3.819873119094476E-5 18-19 0.005118629979586598 20-21 0.0017571416347834591 22-23 0.0 24-25 0.005004033786013763 26-27 0.005004033786013763 28-29 0.0069903678079428905 30-31 0.032927306286594384 32-33 3.819873119094476E-4 34-35 0.0043546553557677024 36-37 0.015546883594714518 38-39 0.007754342431761787 40-41 0.009282291679399576 42-43 0.006799374151988168 44-45 0.0037052769255216424 46-47 0.0012987568604921219 48-49 0.0017571416347834591 50-51 0.002444718796220465 52-53 0.0032850908824212494 54-55 0.0021773276778838512 56-57 0.0012223593981102324 58-59 0.0016043467100196802 60-61 7.639746238188952E-5 62-63 1.909936559547238E-4 64-65 0.009435086604163357 66-67 0.0010313657421555085 68-69 0.0016425454412106247 70-71 0.004927636323631874 72-73 0.012147196518720435 74-75 0.006264591915314941 76-77 0.004125462968622034 78-79 0.009664278991309025 80-81 3.055898495275581E-4 82-83 0.005729809678641715 84-85 9.54968279773619E-4 86-87 0.0 88-89 0.0016425454412106247 90-91 0.0017189429035925142 92-93 2.2919238714566857E-4 94-95 6.111796990551162E-4 96-97 3.055898495275581E-4 98-99 1.5279492476377905E-4 100-101 6.111796990551162E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1308944.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.54708639896917 #Duplication Level Percentage of deduplicated Percentage of total 1 79.8858884604874 48.368577906755455 2 12.156323610920365 14.720599519284486 3 3.5507594172555805 6.449644116555812 4 1.4978990483636405 3.627736923928282 5 0.7538283210021066 2.282105424085221 6 0.48582945987865794 1.7649334969462578 7 0.30083304274947203 1.275019496171195 8 0.22537579247618786 1.0916678063433514 9 0.16293896430679747 0.8878931594677999 >10 0.8921555871317189 9.78905094720307 >50 0.058827124926531915 2.4201676888122554 >100 0.025041201594993262 2.5917270193841135 >500 0.0020235147795443623 0.8237798927500792 >1k 0.0018970451058228398 2.2484336299331265 >5k 3.7940902116456797E-4 1.6586629723795747 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8097 0.6185902529061595 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7201 0.5501381266119865 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6363 0.48611705313596304 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4207 0.3214041242406092 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3302 0.2522644207849992 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2788 0.212996125120708 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2214 0.1691439817135034 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2095 0.16005268369005854 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2055 0.15699678519478297 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1929 0.1473707049346649 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1725 0.13178562260875942 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1585 0.1210899778752949 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1473 0.11253346208852327 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1418 0.10833160165751934 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1384 0.1057340879365351 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 7.639746238188952E-5 7.639746238188952E-5 0.0 0.0 0.0 3 7.639746238188952E-5 7.639746238188952E-5 0.0 0.0 0.0 4 7.639746238188952E-5 7.639746238188952E-5 0.0 0.0 0.0 5 7.639746238188952E-5 7.639746238188952E-5 0.0 0.0 0.0 6 7.639746238188952E-5 7.639746238188952E-5 0.0 0.0 0.0 7 7.639746238188952E-5 7.639746238188952E-5 0.0 0.0 0.0 8 7.639746238188952E-5 7.639746238188952E-5 0.0 0.0 0.0 9 7.639746238188952E-5 7.639746238188952E-5 0.0 0.0 0.0 10-11 7.639746238188952E-5 7.639746238188952E-5 0.0 0.0 0.0 12-13 7.639746238188952E-5 7.639746238188952E-5 0.0 7.639746238188952E-5 0.0 14-15 7.639746238188952E-5 7.639746238188952E-5 0.0 7.639746238188952E-5 0.0 16-17 7.639746238188952E-5 7.639746238188952E-5 0.0 1.1459619357283429E-4 0.0 18-19 1.1459619357283429E-4 7.639746238188952E-5 0.0 1.909936559547238E-4 0.0 20-21 1.5279492476377905E-4 1.5279492476377905E-4 0.0 2.6739111833661336E-4 0.0 22-23 1.5279492476377905E-4 1.5279492476377905E-4 0.0 5.729809678641715E-4 0.0 24-25 3.055898495275581E-4 1.909936559547238E-4 0.0 8.0217335500984E-4 0.0 26-27 3.055898495275581E-4 2.2919238714566857E-4 0.0 0.0013751543228740113 0.0 28-29 4.2018604310039236E-4 2.2919238714566857E-4 0.0 0.0027503086457480227 0.0 30-31 6.111796990551162E-4 2.6739111833661336E-4 0.0 0.004736642667677151 0.0 32-33 6.111796990551162E-4 3.819873119094476E-4 0.0 0.007716143700570842 0.0 34-35 6.111796990551162E-4 3.819873119094476E-4 0.0 0.011345023163710593 0.0 36-37 6.875771614370057E-4 3.819873119094476E-4 0.0 0.014400921658986175 0.0 38-39 7.639746238188952E-4 3.819873119094476E-4 0.0 0.01799160239093498 0.0 40-41 7.639746238188952E-4 3.819873119094476E-4 0.0 0.020932904692637727 0.0 42-43 8.403720862007848E-4 3.819873119094476E-4 0.0 0.02368321333838575 0.0 44-45 9.167695485826743E-4 3.819873119094476E-4 0.0 0.027159297876761725 0.0 46-47 9.931670109645638E-4 3.819873119094476E-4 0.0 0.03223972912515738 0.0 48-49 0.0010695644733464532 3.819873119094476E-4 0.0 0.037129166717598305 0.0 50-51 0.0010695644733464532 3.819873119094476E-4 0.0 0.04175121319170262 0.0 52-53 0.0010695644733464532 3.819873119094476E-4 0.0 0.05053692136561992 0.0 54-55 0.0010695644733464532 3.819873119094476E-4 0.0 0.06757355547678129 0.0 56-57 0.0012223593981102324 3.819873119094476E-4 0.0 0.10592508159248983 0.0 58-59 0.0012223593981102324 3.819873119094476E-4 0.0 0.14893685291349362 0.0 60-61 0.0012605581293011771 3.819873119094476E-4 0.0 0.18690639171729273 0.0 62-63 0.0012987568604921219 3.819873119094476E-4 0.0 0.2388566661369776 0.0 64-65 0.0012987568604921219 3.819873119094476E-4 0.0 0.29947805253700693 0.0 66-67 0.001451551785255901 3.819873119094476E-4 0.0 0.39409630969697707 0.0 68-69 0.001451551785255901 3.819873119094476E-4 0.0 0.5116338055715142 0.0 70-71 0.001451551785255901 3.819873119094476E-4 0.0 0.6073598259360217 0.0 72-73 0.001451551785255901 3.819873119094476E-4 0.0 0.7067911232260509 0.0 74-75 0.001451551785255901 3.819873119094476E-4 0.0 0.8160012957009619 0.0 76-77 0.001451551785255901 3.819873119094476E-4 0.0 0.9399561784155778 0.0 78-79 0.0015279492476377905 3.819873119094476E-4 0.0 1.0391200845872703 0.0 80-81 0.0019863340219291275 3.819873119094476E-4 0.0 1.140232126049701 0.0 82-83 0.0019863340219291275 3.819873119094476E-4 0.0 1.2569292498380373 0.0 84-85 0.002062731484311017 3.819873119094476E-4 0.0 1.4431098656627022 0.0 86-87 0.0021391289466929065 3.819873119094476E-4 0.0 1.6967494407705752 0.0 88-89 0.0021391289466929065 3.819873119094476E-4 0.0 1.979802038895476 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6980 0.0 24.916136 1 TTTGCGT 235 1.7644197E-10 24.254139 3 TACATGG 7150 0.0 23.850311 2 GAGTACT 6770 0.0 22.203648 12-13 ACATGGG 7255 0.0 21.932083 3 GTACTTT 6965 0.0 21.719635 14-15 AGTACTT 7005 0.0 21.221472 12-13 TACACCG 140 4.856327E-4 20.354597 5 TTGCGTT 305 2.0918378E-10 20.24337 4 TAGTACG 120 0.0047536143 19.799025 1 CGCACTA 60 0.004769599 19.788437 94-95 CATGGGG 2375 0.0 19.397575 4 CATGGGA 5070 0.0 19.391066 4 AGAGTAC 11910 0.0 19.299955 10-11 CTGGTCG 305 4.5729394E-9 18.685474 9 GTATCAA 17405 0.0 18.537483 1 TACTTTT 7960 0.0 18.527325 14-15 CTACACG 260 3.2852586E-7 18.266945 4 ACTTTTT 8365 0.0 18.198456 16-17 CTTTGCG 315 6.8048394E-9 18.095741 2 >>END_MODULE