FastQCFastQC Report
Thu 26 May 2016
SRR936885_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936885_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315166
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69300.5269296803597416No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT61660.4688381542710198No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49540.3766824872297489No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT38380.2918262789640243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32510.24719312999271575No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT29020.22065655590244884No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC23070.17541511869984475No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT20600.15663421955859563No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT17270.13131422193091977No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC16710.1270562043118511No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16270.12371061903972579No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC13580.10325692726241402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT55750.022.96632812-13
GTACATG73500.022.1101061
TACATGG72950.021.8249952
GTACTTT59950.021.31488214-15
AGTACTT58600.021.2818512-13
TTTGCGT2056.794435E-720.8652343
GTATAGC5550.020.5480331
GTGTACG1404.8403276E-420.3645691
ACATGGG74100.020.2035183
GTATCAA152300.019.6870881
TACCTGG9450.019.1111342
AGAGTAC110250.018.87404310-11
CTTTGCG2302.04742E-618.5972752
TACTTTT68650.018.19847914-15
ACTTTTT71350.018.14161316-17
GTACCTG10350.017.905081
TATCAAC171000.017.4263342
CAACGCA171400.017.3856685
CATGGGG24150.017.3180984
TCAACGC171900.017.2521554
Produced by FastQC (version 0.11.5)