##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936885_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1315166 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.62038328241454 31.0 31.0 34.0 30.0 34.0 2 31.713836884469337 33.0 31.0 34.0 30.0 34.0 3 31.71848876871817 33.0 31.0 34.0 30.0 34.0 4 35.293031450022276 37.0 35.0 37.0 33.0 37.0 5 35.28901826841631 37.0 35.0 37.0 33.0 37.0 6 35.414443499907996 37.0 35.0 37.0 33.0 37.0 7 35.3652253783933 37.0 35.0 37.0 33.0 37.0 8 35.31984631597836 37.0 35.0 37.0 33.0 37.0 9 36.885918583661685 39.0 37.0 39.0 33.0 39.0 10-11 36.75462070947698 39.0 37.0 39.0 32.0 39.0 12-13 36.640765500324676 39.0 37.0 39.0 32.0 39.0 14-15 37.72965503974403 40.0 37.0 41.0 32.0 41.0 16-17 37.666203733977305 40.0 37.0 41.0 32.0 41.0 18-19 37.63748568621756 40.0 37.0 41.0 32.0 41.0 20-21 37.523967316673335 40.0 37.0 41.0 32.0 41.0 22-23 37.3960716746023 40.0 37.0 41.0 32.0 41.0 24-25 37.51324091407473 40.0 37.0 41.0 32.0 41.0 26-27 37.454266229510196 40.0 37.0 41.0 32.0 41.0 28-29 37.36589069364628 40.0 37.0 41.0 31.5 41.0 30-31 37.17964272190735 39.5 36.5 41.0 31.0 41.0 32-33 36.95817676247714 39.0 36.0 41.0 30.5 41.0 34-35 36.757120774107605 39.0 36.0 41.0 30.0 41.0 36-37 36.55348108147565 39.0 35.0 41.0 30.0 41.0 38-39 36.3845332072149 39.0 35.0 41.0 30.0 41.0 40-41 36.20790341295319 39.0 35.0 40.5 30.0 41.0 42-43 35.9589264777222 39.0 35.0 40.0 29.0 41.0 44-45 35.64197827498582 38.5 35.0 40.0 27.5 41.0 46-47 35.23543149686047 38.0 34.5 40.0 26.0 41.0 48-49 34.92518853133369 38.0 34.0 40.0 24.0 41.0 50-51 34.190690376728114 37.5 33.5 39.5 23.5 40.5 52-53 34.38986713464308 38.0 33.5 39.5 24.0 40.5 54-55 34.80475772640108 38.0 34.0 40.0 24.0 41.0 56-57 34.692731944104395 38.0 34.0 40.0 24.0 41.0 58-59 34.436528924865755 38.0 34.0 40.0 23.0 41.0 60-61 34.119597450055736 38.0 34.0 40.0 22.5 41.0 62-63 33.78542024352819 37.5 33.0 40.0 22.0 41.0 64-65 33.4518057036146 37.0 33.0 40.0 21.5 41.0 66-67 33.048154377470226 36.0 32.5 39.0 20.0 41.0 68-69 32.63170618765996 36.0 32.0 39.0 18.5 41.0 70-71 32.21921719387515 35.0 31.5 39.0 17.5 40.0 72-73 31.80412586700082 35.0 31.0 38.0 14.5 40.0 74-75 31.351774604878777 35.0 31.0 37.0 11.0 39.0 76-77 30.918458202234547 35.0 31.0 37.0 7.5 39.0 78-79 30.47063146401291 35.0 30.0 36.0 7.0 39.0 80-81 30.007289193911646 34.0 30.0 36.0 2.0 37.5 82-83 29.585150847877756 34.0 29.5 35.0 2.0 37.0 84-85 29.171386349707944 34.0 29.0 35.0 2.0 36.5 86-87 28.845451068534313 34.0 29.0 35.0 2.0 36.0 88-89 28.474516524910165 34.0 29.0 35.0 2.0 36.0 90-91 28.057915122501647 34.0 27.5 35.0 2.0 35.5 92-93 27.667026443810133 34.0 27.0 35.0 2.0 35.0 94-95 27.22143022249663 34.0 26.0 35.0 2.0 35.0 96-97 26.673269001783808 33.0 25.0 35.0 2.0 35.0 98-99 26.004787228380295 33.0 24.5 35.0 2.0 35.0 100-101 23.401643974981106 30.0 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4312400302798629 1101 2 0.5638976920404808 1101 3 0.5692518976132739 1101 4 0.4953881207514996 1101 5 0.5875212316418299 1101 6 0.334747518822077 1101 7 0.38564287993053625 1101 8 0.4634211280153764 1101 9 0.5516789584286244 1101 10-11 0.6339021417851285 1101 12-13 0.7110636738305516 1101 14-15 0.7432895222834262 1101 16-17 0.8806348071645544 1101 18-19 0.9148359414714164 1101 20-21 0.9774096132635535 1101 22-23 0.8575338889415036 1101 24-25 0.8266593129911044 1101 26-27 0.8094849041989178 1101 28-29 0.6096527429589003 1101 30-31 0.7882371603605236 1101 32-33 0.9314006150381218 1101 34-35 0.8708347972770909 1101 36-37 0.8275506973420832 1101 38-39 0.9197186340085182 1101 40-41 0.888975641056561 1101 42-43 0.8316316042653611 1101 44-45 1.0487200686092137 1101 46-47 1.1534929125177555 1101 48-49 1.2027138439349088 1101 50-51 1.202774689179975 1101 52-53 1.264345699320657 1101 54-55 1.2502450310677276 1101 56-57 1.2767443491299488 1101 58-59 1.2008120121519426 1101 60-61 1.269904396020216 1101 62-63 1.2723924087080078 1101 64-65 1.3208700671514748 1101 66-67 1.1822939904133776 1101 68-69 1.2966678668610498 1101 70-71 1.2579940611554292 1101 72-73 1.1061522971381095 1101 74-75 1.1773563833202338 1101 76-77 1.3398860061176556 1101 78-79 1.4562094509001469 1101 80-81 1.435191979657663 1101 82-83 1.2799003781413738 1101 84-85 1.25515844018744 1101 86-87 1.2846926667399394 1101 88-89 1.460187509807703 1101 90-91 1.5596459516481538 1101 92-93 1.4271745064510064 1101 94-95 1.5353913284309613 1101 96-97 1.6088257286872185 1101 98-99 1.555557941766665 1101 100-101 1.8762010731152543 1102 1 0.40082063771957266 1102 2 0.583384778703067 1102 3 0.5036264576633762 1102 4 0.43752160118971517 1102 5 0.5236570278326198 1102 6 0.3815179633833594 1102 7 0.5033612602828441 1102 8 0.3811458292804204 1102 9 0.678956282728798 1102 10-11 0.6083089097587902 1102 12-13 0.5921946000453886 1102 14-15 0.5786333368393386 1102 16-17 0.8004296073518802 1102 18-19 0.7720644798178071 1102 20-21 0.8736338735012765 1102 22-23 0.7632323354335426 1102 24-25 0.4272142603982374 1102 26-27 0.6421078531345543 1102 28-29 0.7315960788493285 1102 30-31 0.5615340335199122 1102 32-33 0.9199647909778648 1102 34-35 0.6811715008061654 1102 36-37 0.588071425626147 1102 38-39 0.5932384629990963 1102 40-41 0.5094227881700562 1102 42-43 0.7363474361405693 1102 44-45 0.7175545210314169 1102 46-47 0.7092189411744698 1102 48-49 0.9362457113911162 1102 50-51 0.5990837071112622 1102 52-53 0.7388587735540284 1102 54-55 1.0487558814890718 1102 56-57 1.1052163259379526 1102 58-59 0.8869334426319568 1102 60-61 0.7105517436790407 1102 62-63 0.9974930405119693 1102 64-65 1.1228990801409537 1102 66-67 1.0835462494213957 1102 68-69 1.1527988649044332 1102 70-71 1.1204057479484604 1102 72-73 0.9145079691516926 1102 74-75 0.8639619382532793 1102 76-77 1.2036609988629756 1102 78-79 1.0958513424620158 1102 80-81 1.0589073259718553 1102 82-83 1.3237574565580452 1102 84-85 1.1222878263980824 1102 86-87 0.9809282758891733 1102 88-89 1.1888415001711827 1102 90-91 1.1036474054580871 1102 92-93 1.063407414846619 1102 94-95 1.1111359750488425 1102 96-97 1.2661582747796025 1102 98-99 1.2070608824276583 1102 100-101 1.3227125039113155 1103 1 0.18778588939529328 1103 2 0.40405652732230735 1103 3 0.22313812477654338 1103 4 0.4818100872386992 1103 5 0.34570615337416655 1103 6 0.29645884982959103 1103 7 0.3184842635598599 1103 8 0.3930010125743806 1103 9 0.4313748104779407 1103 10-11 0.49663584329561417 1103 12-13 0.6443969147963102 1103 14-15 0.8210888832845384 1103 16-17 0.6968394974052714 1103 18-19 0.7948331427338999 1103 20-21 0.667999481833732 1103 22-23 0.5839094961428515 1103 24-25 0.48233978029713853 1103 26-27 0.6932527807884483 1103 28-29 0.7159077827647096 1103 30-31 0.7341979724931846 1103 32-33 0.827456611956741 1103 34-35 0.705736301113582 1103 36-37 0.6877066507555654 1103 38-39 0.721448170665056 1103 40-41 0.7625793897022248 1103 42-43 0.6690020933793832 1103 44-45 0.6291020270050112 1103 46-47 0.9107239081201328 1103 48-49 0.9133146857729884 1103 50-51 0.9215576817231437 1103 52-53 1.0355475215481675 1103 54-55 1.0909553007718102 1103 56-57 1.0905015295543095 1103 58-59 1.1587651374550205 1103 60-61 1.156979493629656 1103 62-63 1.2341813284784564 1103 64-65 1.1168206264833245 1103 66-67 1.1389883765892748 1103 68-69 1.1721545285213892 1103 70-71 1.0843213139313121 1103 72-73 0.9155430313771973 1103 74-75 0.9589772679983959 1103 76-77 0.8895920001266973 1103 78-79 0.6992493232112089 1103 80-81 0.8807135839716018 1103 82-83 0.851857028191116 1103 84-85 0.8595139408150771 1103 86-87 0.8545052344569193 1103 88-89 0.9431359936401407 1103 90-91 0.7868764207553589 1103 92-93 0.988092769460291 1103 94-95 1.107674266939096 1103 96-97 1.0048436239811842 1103 98-99 1.2282559801564794 1103 100-101 1.3979439384128476 1104 1 0.3267453781947758 1104 2 0.4398099512750555 1104 3 0.4204771655552797 1104 4 0.3648053127410975 1104 5 0.4131909500978779 1104 6 0.3538544334193148 1104 7 0.33633718022224457 1104 8 0.3842211848267283 1104 9 0.45719594174575207 1104 10-11 0.5148379722687864 1104 12-13 0.5610502903866887 1104 14-15 0.6186036235168828 1104 16-17 0.6010382770272216 1104 18-19 0.6390960282179847 1104 20-21 0.7164126559485453 1104 22-23 0.66501100052119 1104 24-25 0.5899280416185562 1104 26-27 0.6330364878153958 1104 28-29 0.5498833752037129 1104 30-31 0.4041801195765231 1104 32-33 0.4327116462734466 1104 34-35 0.566603170833595 1104 36-37 0.38753583898807875 1104 38-39 0.4113997311231543 1104 40-41 0.48105510047467703 1104 42-43 0.3827508971825182 1104 44-45 0.22339449585243898 1104 46-47 0.28003747119186073 1104 48-49 0.5704724448460752 1104 50-51 0.42526010395748415 1104 52-53 0.3363479444616502 1104 54-55 0.39037173677399295 1104 56-57 0.4844786140238142 1104 58-59 0.482306806265413 1104 60-61 0.5905641998025501 1104 62-63 0.6924707155111705 1104 64-65 0.69456313288876 1104 66-67 0.5943039924891735 1104 68-69 0.3904701032618263 1104 70-71 0.3013789526129713 1104 72-73 0.280740005127754 1104 74-75 0.2745365736382688 1104 76-77 0.300710071188508 1104 78-79 0.25067935727066626 1104 80-81 0.39076099452694635 1104 82-83 0.3858740373388052 1104 84-85 0.5531203328795335 1104 86-87 0.508347084368939 1104 88-89 0.6185177476506141 1104 90-91 0.3946492565393953 1104 92-93 0.573090917284496 1104 94-95 0.8060783087982664 1104 96-97 0.8518294651262401 1104 98-99 0.7431576347668489 1104 100-101 0.9629025731534924 1105 1 0.25027284850280296 1105 2 0.4292712100865863 1105 3 0.31255897796014764 1105 4 0.4124756444143429 1105 5 0.4281951865302105 1105 6 0.24506617621861437 1105 7 0.38002415713368265 1105 8 0.3529343931360458 1105 9 0.5551061737150533 1105 10-11 0.4914757105065348 1105 12-13 0.5219390298510334 1105 14-15 0.5198436420389569 1105 16-17 0.6942422513918842 1105 18-19 0.7153530417302392 1105 20-21 0.5997208046768776 1105 22-23 0.6474134886862686 1105 24-25 0.47375496857951305 1105 26-27 0.36684814391561105 1105 28-29 0.3383634812130438 1105 30-31 0.05521570560811995 1105 32-33 0.317639553032528 1105 34-35 0.03387857834389507 1105 36-37 0.15847662253101902 1105 38-39 0.45470647591751856 1105 40-41 0.4700126671861753 1105 42-43 0.3594406671836481 1105 44-45 0.2570870638693492 1105 46-47 0.07303725937025263 1105 48-49 0.07091363725399447 1105 50-51 0.10469179394916495 1105 52-53 0.2485660492985673 1105 54-55 0.4325892012199759 1105 56-57 0.44020235430095767 1105 58-59 0.5581919004275093 1105 60-61 0.2969009296708762 1105 62-63 0.436623145794762 1105 64-65 0.5238607718166577 1105 66-67 0.4095779936024684 1105 68-69 0.46895524619215934 1105 70-71 0.2830985623685933 1105 72-73 0.3210301391665027 1105 74-75 0.14473548891454868 1105 76-77 0.24245512416853998 1105 78-79 0.29172533733973083 1105 80-81 0.3907064997214995 1105 82-83 0.1779889776592931 1105 84-85 0.23456032578597075 1105 86-87 0.11509194030560721 1105 88-89 0.4032361726830054 1105 90-91 0.14860828876091148 1105 92-93 0.015620141278468225 1105 94-95 0.2996374538271098 1105 96-97 0.2223516546721065 1105 98-99 -0.062243878033577005 1105 100-101 -0.07196486592316376 1106 1 0.19104696314515124 1106 2 0.38618949998388885 1106 3 0.3754467996835551 1106 4 0.3745828765588044 1106 5 0.5003817366480163 1106 6 0.37762251715999895 1106 7 0.369389073768879 1106 8 0.47545458234808535 1106 9 0.40107462638506775 1106 10-11 0.5587296179509096 1106 12-13 0.5866650141313201 1106 14-15 0.7211871913833718 1106 16-17 0.5780639028604355 1106 18-19 0.31544027351498016 1106 20-21 0.5372484580519412 1106 22-23 0.6172816517827329 1106 24-25 0.7927103454747808 1106 26-27 0.4462740328704484 1106 28-29 0.732643953883418 1106 30-31 0.6363889281480937 1106 32-33 0.5217898075335015 1106 34-35 0.43183177894103153 1106 36-37 0.5442632373981908 1106 38-39 0.3059493040259369 1106 40-41 0.30345301683274783 1106 42-43 0.3397325789086807 1106 44-45 0.5216700599664037 1106 46-47 0.6847380093837359 1106 48-49 0.4799506702941585 1106 50-51 0.25354788848324006 1106 52-53 0.37576617879729923 1106 54-55 0.3236205147328164 1106 56-57 0.537936126491104 1106 58-59 0.40829801744400385 1106 60-61 0.35768079981242096 1106 62-63 0.4147927171899326 1106 64-65 0.41270859282062844 1106 66-67 0.40111471188877346 1106 68-69 0.2933270874123295 1106 70-71 0.4405861071086008 1106 72-73 0.31212979923233064 1106 74-75 0.5471258196758946 1106 76-77 0.5684870671890998 1106 78-79 0.6606618290032493 1106 80-81 0.5184920714044452 1106 82-83 0.584144663912646 1106 84-85 0.6794028589250587 1106 86-87 1.026327753899377 1106 88-89 1.0208075318801164 1106 90-91 1.107283804507599 1106 92-93 0.7657154667339618 1106 94-95 0.6556141800272215 1106 96-97 0.7531786479633524 1106 98-99 0.891186939067449 1106 100-101 0.6316467098246008 1107 1 0.17483715864637261 1107 2 0.20508910574906736 1107 3 0.1993138796329923 1107 4 0.11664132490134449 1107 5 0.2939106289232072 1107 6 0.11677528748213462 1107 7 0.029307571636081775 1107 8 0.0765501280140981 1107 9 0.2962416422641283 1107 10-11 0.24194058264293972 1107 12-13 0.16171912762246166 1107 14-15 0.056910176485565955 1107 16-17 0.3462659496491227 1107 18-19 0.2536686411057616 1107 20-21 0.41517534856086513 1107 22-23 0.3330683609320886 1107 24-25 0.19884263059370966 1107 26-27 -0.067991726421198 1107 28-29 -0.03271879410068124 1107 30-31 0.2168754589393913 1107 32-33 0.08180152490812276 1107 34-35 0.06751435930317484 1107 36-37 0.09363754113793732 1107 38-39 0.03334573801902252 1107 40-41 0.1128762680290194 1107 42-43 0.09812810668116612 1107 44-45 0.04720787591703868 1107 46-47 0.1655668260443406 1107 48-49 -0.01741811531252324 1107 50-51 -0.0070525836750121584 1107 52-53 -0.022694400606667386 1107 54-55 0.0193303971550165 1107 56-57 0.1776487910682718 1107 58-59 0.2828519779743388 1107 60-61 0.33790325676694266 1107 62-63 0.20879059305502778 1107 64-65 0.35081920945468426 1107 66-67 0.29947168199503693 1107 68-69 0.22597094271717566 1107 70-71 0.12699366394942047 1107 72-73 0.15673922969865828 1107 74-75 0.1194993672697926 1107 76-77 -0.05626920564679594 1107 78-79 -0.06417034702289115 1107 80-81 -0.07011985261155473 1107 82-83 -0.01723111748616546 1107 84-85 0.02127082777877476 1107 86-87 0.08389065542148089 1107 88-89 -0.21809732650127245 1107 90-91 0.031688267499742295 1107 92-93 -0.008492167453066912 1107 94-95 -0.06037715026998569 1107 96-97 -0.27400999022374606 1107 98-99 -0.4176143677372899 1107 100-101 -0.6820285630516949 1108 1 0.14593965516088758 1108 2 0.2788378104294509 1108 3 0.2010626992074016 1108 4 0.4155646353516218 1108 5 0.36819624220319014 1108 6 0.13481088695274224 1108 7 0.07452444514881762 1108 8 0.17444591029876477 1108 9 0.29707904291947074 1108 10-11 0.2263509001260573 1108 12-13 0.306729191896828 1108 14-15 0.3900650005982911 1108 16-17 0.31537326729353055 1108 18-19 0.28590469971054944 1108 20-21 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-2.9030030435228262 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2664.0 3 1040.0 4 995.0 5 1195.0 6 1330.0 7 1617.0 8 1941.0 9 2532.0 10 3158.0 11 3930.0 12 5281.0 13 6382.0 14 7261.0 15 9007.0 16 11637.0 17 14910.0 18 13009.0 19 8468.0 20 8270.0 21 8702.0 22 9625.0 23 10681.0 24 11944.0 25 13684.0 26 15939.0 27 18925.0 28 22417.0 29 27103.0 30 32714.0 31 40562.0 32 51046.0 33 65350.0 34 87466.0 35 122157.0 36 177478.0 37 248722.0 38 211937.0 39 34078.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.2268070248025 19.390840011651722 14.867279117328744 21.51507384621703 2 14.932158008761704 21.288537771826125 40.43957564821173 23.339728571200443 3 19.668140667596948 26.145861454051854 29.925362068113294 24.260635810237908 4 12.188075138793824 17.212183436002736 38.88934519735341 31.710396227850023 5 13.1038197569543 38.43235293893882 33.864956181627704 14.59887112247918 6 29.676054045254762 37.32179016919189 18.09414560452517 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27.322041424786363 30.202173852708913 21.236447637705915 30-31 21.623315992049672 26.702522723367238 29.957054850870534 21.717106433712548 32-33 21.540214468038513 26.835345634488856 30.02837675387272 21.59606314359991 34-35 22.110732155559248 26.73433627504392 29.854364225920026 21.300567343476803 36-37 21.955555420380392 26.42784256892286 30.23709554535321 21.37950646534354 38-39 21.5893010980388 27.054060979513565 30.088323652657508 21.26831426979013 40-41 21.753704574598842 26.982480138416143 29.893142644893473 21.37067264209154 42-43 21.470288116158898 27.156613167635367 29.257567877466233 22.115530838739502 44-45 21.829225035518398 26.90243456421958 29.237125269975937 22.031215130286082 46-47 22.32940353491767 27.281633863431587 28.823227503773293 21.565735097877454 48-49 22.12564768661491 27.35261030679502 28.635114394696014 21.886627611894053 50-51 21.63171285102086 27.667195878232675 28.91909298003899 21.78199829070747 52-53 22.06091956076844 28.159224672577988 27.935981958095606 21.843873808557966 54-55 21.71557683943513 27.456559493647788 28.440681239601805 22.38718242731528 56-57 22.129385925726826 27.706698470184392 27.988033381527778 22.175882222561007 58-59 22.621116458623145 27.376583422645105 28.098934759888515 21.903365358843235 60-61 22.204889539952255 27.504850341814603 28.815991927692934 21.474268190540208 62-63 21.826960730267235 27.590285407961918 28.795066666159748 21.787687195611095 64-65 21.741299150017355 27.92525840316406 28.40140380011474 21.93203864670384 66-67 21.938605183129695 27.689378759038995 27.946565657564236 22.42545040026707 68-69 21.723642959129545 27.653575522977153 28.39257841092515 22.230203106968148 70-71 22.29089887140514 27.587692661072055 28.04689618349583 22.07451228402698 72-73 21.9753978363785 27.341901629370497 28.574481434302335 22.108219099948666 74-75 21.58983918095299 28.374872803435107 27.911527469134395 22.123760546477513 76-77 21.900701667649127 27.65072543687844 27.935956188556542 22.512616706915896 78-79 21.92474935782747 27.77010698755963 28.01446528085423 22.29067837375867 80-81 22.1540011099138 27.97313689101658 27.696609361693064 22.176252637376557 82-83 22.05461196342468 27.714301144709232 28.092878979079718 22.138207912786374 84-85 21.90903138026468 27.98050150927353 27.802917548565603 22.307549561896188 86-87 21.84370017647012 28.03133342737975 27.874179581255156 22.250786814894976 88-89 21.96455656542343 27.821464924825197 27.457121596448886 22.756856913302485 90-91 21.99334891211551 27.488919607019195 27.81641628432735 22.701315196537937 92-93 22.28074392241009 27.77542828934508 27.88906577663312 22.05476201161171 94-95 21.952903663196285 27.7299255391029 27.829546409447097 22.48762438825371 96-97 21.451248407192818 27.746691657245766 28.244563921619626 22.55749601394179 98-99 21.708178015958552 27.962413512212297 27.49400850788078 22.83539996394837 100-101 21.787100096683474 27.623062241793882 27.70217381878428 22.887663842738355 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 520.0 1 354.5 2 227.0 3 293.0 4 280.0 5 284.0 6 489.5 7 960.0 8 1668.0 9 2046.0 10 1867.0 11 1690.5 12 1715.5 13 1806.5 14 1957.5 15 2059.0 16 2119.5 17 2254.0 18 2395.5 19 2553.5 20 2829.5 21 3252.0 22 3815.0 23 4587.5 24 5451.5 25 6666.5 26 8442.0 27 10313.0 28 11936.0 29 13894.5 30 16300.0 31 18743.0 32 21446.0 33 24164.5 34 27178.5 35 30140.5 36 33621.5 37 37071.0 38 38961.0 39 40014.0 40 40430.5 41 41238.0 42 41974.5 43 42937.5 44 44825.5 45 46217.5 46 47179.0 47 52024.0 48 63768.0 49 63429.5 50 51041.0 51 44981.0 52 42505.0 53 40936.5 54 39252.5 55 36016.0 56 32295.0 57 28934.0 58 26158.0 59 23193.0 60 19672.5 61 15914.0 62 12293.5 63 9189.5 64 6510.5 65 4699.0 66 3351.5 67 2427.5 68 1800.5 69 1226.5 70 827.5 71 558.0 72 369.5 73 274.0 74 213.0 75 147.0 76 99.0 77 50.0 78 23.0 79 16.5 80 11.0 81 8.5 82 5.5 83 2.5 84 2.0 85 3.0 86 4.0 87 4.5 88 5.0 89 4.0 90 2.5 91 1.5 92 1.5 93 1.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025776213801147536 2 0.009504503613992455 3 0.011481440365702885 4 0.020225583690575942 5 0.05801549005980994 6 0.04288432030633395 7 0.016956034447362538 8 0.050639995255351795 9 0.027068826292650512 10-11 0.02288684470249383 12-13 0.03543278947296387 14-15 0.018894953184616998 16-17 0.01691801643290657 18-19 0.015891530042595383 20-21 0.013724503218605104 22-23 0.007337476790002175 24-25 0.0016347746216067022 26-27 0.0 28-29 0.0026992790263738573 30-31 0.0 32-33 3.801801445596982E-4 34-35 1.1405404336790945E-4 36-37 0.0 38-39 2.281080867358189E-4 40-41 1.1405404336790945E-4 42-43 1.5207205782387926E-4 44-45 1.900900722798491E-4 46-47 7.603602891193963E-5 48-49 3.421621301037284E-4 50-51 1.5207205782387926E-4 52-53 4.1819815901566803E-4 54-55 0.0025852249830059476 56-57 5.702702168395473E-4 58-59 0.0012545944770470038 60-61 0.003991891517876831 62-63 0.0023571168962701287 64-65 0.0016347746216067022 66-67 0.012850088886117798 68-69 0.01661387231725881 70-71 0.029616033261200488 72-73 0.01832468296777745 74-75 0.02068179986404758 76-77 0.03353188875016538 78-79 0.022316574485654283 80-81 0.04938540077830479 82-83 0.03870233871617727 84-85 0.02243062852902219 86-87 0.016157656143787173 88-89 0.015017115710108078 90-91 0.026118375931251263 92-93 0.03490053727058029 94-95 0.013724503218605104 96-97 0.02056774582067967 98-99 0.029273871131096762 100-101 0.04311242839306977 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1315166.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.67743147902235 #Duplication Level Percentage of deduplicated Percentage of total 1 81.06823201752069 50.81148557403638 2 11.585134623699608 14.522529631043632 3 3.3218060217664998 6.246068079476207 4 1.3600492391387893 3.4097757197687177 5 0.7150460623556042 2.240862528881906 6 0.42062976748627173 1.5818396057794737 7 0.28281239962294014 1.24081683591493 8 0.18641264633102672 0.9347092693788923 9 0.15498979118124387 0.8742925815039354 >10 0.8268819050333415 9.426094776877871 >50 0.052577000939144525 2.2533391013743986 >100 0.021778526382037353 2.366208245556853 >500 0.0015816660352299448 0.6866226307465997 >1k 0.001824999271419167 2.406687053127797 >5k 2.4333323618922227E-4 0.9986683665323711 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6930 0.5269296803597416 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6166 0.4688381542710198 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4954 0.3766824872297489 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3838 0.2918262789640243 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3251 0.24719312999271575 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2902 0.22065655590244884 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2307 0.17541511869984475 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2060 0.15663421955859563 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1727 0.13131422193091977 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1671 0.1270562043118511 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1627 0.12371061903972579 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1358 0.10325692726241402 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 7.603602891193963E-5 0.0 0.0 0.0 6 7.603602891193963E-5 7.603602891193963E-5 0.0 0.0 0.0 7 1.5207205782387926E-4 7.603602891193963E-5 0.0 0.0 0.0 8 1.5207205782387926E-4 7.603602891193963E-5 0.0 0.0 0.0 9 1.5207205782387926E-4 7.603602891193963E-5 0.0 0.0 0.0 10-11 1.5207205782387926E-4 7.603602891193963E-5 0.0 1.5207205782387926E-4 0.0 12-13 1.5207205782387926E-4 7.603602891193963E-5 0.0 1.5207205782387926E-4 0.0 14-15 1.5207205782387926E-4 7.603602891193963E-5 0.0 1.5207205782387926E-4 0.0 16-17 1.9009007227984906E-4 7.603602891193963E-5 0.0 1.5207205782387926E-4 0.0 18-19 2.281080867358189E-4 7.603602891193963E-5 0.0 1.5207205782387926E-4 0.0 20-21 2.281080867358189E-4 2.281080867358189E-4 0.0 1.9009007227984906E-4 0.0 22-23 3.041441156477585E-4 2.281080867358189E-4 0.0 3.041441156477585E-4 0.0 24-25 3.041441156477585E-4 2.281080867358189E-4 0.0 6.08288231295517E-4 0.0 26-27 3.041441156477585E-4 2.281080867358189E-4 0.0 8.744143324873057E-4 0.0 28-29 3.041441156477585E-4 2.281080867358189E-4 0.0 0.0017868466794305814 0.0 30-31 3.041441156477585E-4 2.281080867358189E-4 0.0 0.003877837474508921 0.0 32-33 3.041441156477585E-4 2.281080867358189E-4 0.0 0.0064250444430589 0.0 34-35 4.18198159015668E-4 2.281080867358189E-4 0.0 0.009504503613992455 0.0 36-37 6.843242602074568E-4 2.281080867358189E-4 0.0 0.012089728596998401 0.0 38-39 7.603602891193964E-4 2.281080867358189E-4 0.0 0.014028647334252863 0.0 40-41 7.603602891193964E-4 2.281080867358189E-4 0.0 0.016499818273890902 0.0 42-43 7.983783035753662E-4 2.281080867358189E-4 0.0 0.02007351163275206 0.0 44-45 8.36396318031336E-4 2.281080867358189E-4 0.0 0.023076934774773676 0.0 46-47 9.124323469432756E-4 2.281080867358189E-4 0.0 0.027372970408298267 0.0 48-49 9.124323469432756E-4 2.281080867358189E-4 0.0 0.033417834706797475 0.0 50-51 9.124323469432756E-4 2.281080867358189E-4 0.0 0.03748576225358624 0.0 52-53 9.124323469432756E-4 3.801801445596982E-4 0.0 0.04683819380975482 3.8018014455969815E-5 54-55 9.124323469432756E-4 3.801801445596982E-4 0.0 0.06626539919675539 7.603602891193963E-5 56-57 0.0011405404336790946 3.801801445596982E-4 0.0 0.10420737762381327 7.603602891193963E-5 58-59 0.0011405404336790946 4.562161734716378E-4 0.0 0.14602719352538007 7.603602891193963E-5 60-61 0.0011405404336790946 4.562161734716378E-4 0.0 0.18393115393798198 7.603602891193963E-5 62-63 0.0011405404336790946 4.562161734716378E-4 0.0 0.23236610435488753 7.603602891193963E-5 64-65 0.0011405404336790946 4.562161734716378E-4 0.0 0.2936131256434549 7.603602891193963E-5 66-67 0.001216576462591034 4.562161734716378E-4 0.0 0.38489437835223844 7.603602891193963E-5 68-69 0.001216576462591034 4.562161734716378E-4 0.0 0.4972756290840852 7.603602891193963E-5 70-71 0.001216576462591034 4.562161734716378E-4 0.0 0.5970348990165499 7.603602891193963E-5 72-73 0.0012545944770470038 5.322522023835774E-4 0.0 0.6979727273971499 7.603602891193963E-5 74-75 0.0012926124915029738 5.322522023835774E-4 0.0 0.804955420076249 7.603602891193963E-5 76-77 0.0012926124915029738 5.322522023835774E-4 0.0 0.9297305435207419 7.603602891193963E-5 78-79 0.0012926124915029738 5.322522023835774E-4 0.0 1.0340519751879231 7.603602891193963E-5 80-81 0.0015207205782387927 5.322522023835774E-4 0.0 1.1406544877224625 7.603602891193963E-5 82-83 0.0015967566071507322 5.322522023835774E-4 0.0 1.2597269089985599 7.603602891193963E-5 84-85 0.0015967566071507322 5.322522023835774E-4 0.0 1.449931035321777 7.603602891193963E-5 86-87 0.0015967566071507322 5.322522023835774E-4 0.0 1.6898247065389465 7.603602891193963E-5 88-89 0.0015967566071507322 5.322522023835774E-4 0.0 1.9663677436916709 7.603602891193963E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 5575 0.0 22.966328 12-13 GTACATG 7350 0.0 22.110106 1 TACATGG 7295 0.0 21.824995 2 GTACTTT 5995 0.0 21.314882 14-15 AGTACTT 5860 0.0 21.28185 12-13 TTTGCGT 205 6.794435E-7 20.865234 3 GTATAGC 555 0.0 20.548033 1 GTGTACG 140 4.8403276E-4 20.364569 1 ACATGGG 7410 0.0 20.203518 3 GTATCAA 15230 0.0 19.687088 1 TACCTGG 945 0.0 19.111134 2 AGAGTAC 11025 0.0 18.874043 10-11 CTTTGCG 230 2.04742E-6 18.597275 2 TACTTTT 6865 0.0 18.198479 14-15 ACTTTTT 7135 0.0 18.141613 16-17 GTACCTG 1035 0.0 17.90508 1 TATCAAC 17100 0.0 17.426334 2 CAACGCA 17140 0.0 17.385668 5 CATGGGG 2415 0.0 17.318098 4 TCAACGC 17190 0.0 17.252155 4 >>END_MODULE