FastQCFastQC Report
Thu 26 May 2016
SRR936885_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936885_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315166
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78360.595818322553959No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT70960.5395516611591237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62880.4781145497982764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40530.30817402518009135No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33160.2521354718719918No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT27950.21252070080887128No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC21890.16644286728823585No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT21100.16043602100419263No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC20280.15420106663341357No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC18520.1408187255449122No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT16180.12302629477951832No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15460.11755170069785868No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG14860.11298953896314229No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA13970.10622233238997966No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC13310.10120395448179165No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG700.00838304227.1488025
TCCCGTA908.956666E-426.3846322
CCCGTAC950.001227802724.9959683
GAGTACT68700.024.1609512-13
GACCGTA1000.00165166623.7552017
GTACTTT71050.023.02760114-15
AGTACTT69800.022.31731612-13
GTACATG72900.021.0489621
TACATGG73050.020.089162
AGAGTAC119050.019.73447610-11
ACATGGG72700.019.4019583
ACTTTTT86100.019.25140216-17
TACTTTT82650.018.7326114-15
GTATCAA176650.018.5293961
AGTACAT56200.017.492732
CTTACAC6050.017.2699413
CAACGCA197650.016.4898225
GTATTAC2606.6153225E-616.4446581
TCAACGC197600.016.3915864
ATCAACG198200.016.3180033
Produced by FastQC (version 0.11.5)