##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936885_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1315166 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10668767288692 31.0 31.0 34.0 31.0 34.0 2 32.21516599425472 33.0 31.0 34.0 30.0 34.0 3 32.324222189442246 34.0 31.0 34.0 30.0 34.0 4 35.99236446197666 37.0 35.0 37.0 35.0 37.0 5 35.900575288594744 37.0 35.0 37.0 35.0 37.0 6 35.95301353593387 37.0 35.0 37.0 35.0 37.0 7 35.8996704598507 37.0 35.0 37.0 35.0 37.0 8 35.866531677370006 37.0 35.0 37.0 35.0 37.0 9 37.52729389293823 39.0 37.0 39.0 35.0 39.0 10-11 37.437547807653175 39.0 37.0 39.0 34.0 39.0 12-13 37.343544465109346 39.0 37.0 39.0 34.0 39.0 14-15 38.48028994058545 40.0 38.0 41.0 34.0 41.0 16-17 38.404001472057516 40.0 38.0 41.0 34.0 41.0 18-19 38.60740963498144 40.0 38.0 41.0 34.0 41.0 20-21 38.65064258048034 40.0 38.0 41.0 34.0 41.0 22-23 38.56790017381836 40.0 38.0 41.0 34.0 41.0 24-25 38.45892951916336 40.0 38.0 41.0 34.0 41.0 26-27 38.26729515513631 40.0 38.0 41.0 34.0 41.0 28-29 38.18694484194391 40.0 38.0 41.0 33.5 41.0 30-31 38.16439255576863 40.0 37.5 41.0 34.0 41.0 32-33 37.96903660830648 40.0 37.0 41.0 33.0 41.0 34-35 37.8035369679569 40.0 37.0 41.0 33.0 41.0 36-37 37.62389652713041 40.0 37.0 41.0 33.0 41.0 38-39 37.46294460167006 40.0 36.5 41.0 33.0 41.0 40-41 37.281972009617036 40.0 36.0 41.0 32.0 41.0 42-43 37.007015464207555 39.5 36.0 41.0 31.0 41.0 44-45 36.70763272469027 39.0 36.0 41.0 31.0 41.0 46-47 36.307785100892204 39.0 35.5 41.0 30.0 41.0 48-49 35.9044774575985 39.0 35.0 40.0 29.0 41.0 50-51 35.61047274640616 39.0 35.0 40.0 28.0 41.0 52-53 35.37756906732686 39.0 35.0 40.0 27.5 41.0 54-55 35.104286455093884 38.0 34.5 40.0 26.5 41.0 56-57 34.80931076381232 38.0 34.0 40.0 26.0 41.0 58-59 34.45490569251334 38.0 34.0 40.0 25.5 41.0 60-61 34.095169735227344 37.5 33.5 40.0 25.0 41.0 62-63 33.7356637869288 37.0 33.0 39.5 23.5 41.0 64-65 33.34529709557577 36.0 32.5 39.0 23.0 40.0 66-67 32.944523733125706 36.0 32.0 39.0 22.0 40.0 68-69 32.61772962500551 35.5 32.0 38.5 22.0 40.0 70-71 32.12912742573941 35.0 31.0 38.0 20.5 40.0 72-73 31.613369338927555 35.0 31.0 37.0 18.5 39.0 74-75 31.086245386513944 34.5 30.5 37.0 17.5 39.0 76-77 29.68581532673442 33.0 28.5 35.0 14.0 37.5 78-79 30.44046797134354 34.0 30.0 36.0 14.5 38.0 80-81 30.213454043063763 34.0 30.0 35.5 10.0 37.0 82-83 29.865877387341218 34.0 30.0 35.0 7.5 37.0 84-85 29.481959311600207 34.0 29.0 35.0 6.5 36.0 86-87 29.14163155069398 34.0 29.0 35.0 2.0 36.0 88-89 28.725345697805448 34.0 29.0 35.0 2.0 35.5 90-91 28.229003030796115 33.0 28.5 35.0 2.0 35.0 92-93 27.722345316104583 33.0 27.0 35.0 2.0 35.0 94-95 27.09795113316494 33.0 26.0 34.5 2.0 35.0 96-97 26.40163256957677 32.0 25.0 34.0 2.0 35.0 98-99 25.539961115174815 32.0 23.5 34.0 2.0 35.0 100-101 21.915825074553325 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29227006034908243 1101 2 0.1885219105918452 1101 3 0.2713327161095478 1101 4 0.24142648186050053 1101 5 0.3066077492135051 1101 6 0.2133247717443325 1101 7 0.28904063046738315 1101 8 0.34454239925634766 1101 9 0.47742760657832406 1101 10-11 0.4198331945677083 1101 12-13 0.467607102453627 1101 14-15 0.6192293489162068 1101 16-17 0.5683016832793228 1101 18-19 0.4915244918389945 1101 20-21 0.5093107729426407 1101 22-23 0.4792841529926264 1101 24-25 0.49517598553928366 1101 26-27 0.4861684089495597 1101 28-29 0.6260961618661511 1101 30-31 0.5607697272132128 1101 32-33 0.5115437728654726 1101 34-35 0.6675277408146627 1101 36-37 0.5212915208888944 1101 38-39 0.5670982709474757 1101 40-41 0.7734908585145845 1101 42-43 0.5608264222491997 1101 44-45 0.5125100400861129 1101 46-47 0.5154066223318452 1101 48-49 0.6161623922072224 1101 50-51 0.46850002131613877 1101 52-53 0.5653454353362264 1101 54-55 0.6922728753507741 1101 56-57 0.5983003249191015 1101 58-59 0.5683170464983718 1101 60-61 0.8110434031800224 1101 62-63 0.62214112278415 1101 64-65 0.599934796689908 1101 66-67 0.36558708839262266 1101 68-69 0.5175248096673997 1101 70-71 0.3584937406910669 1101 72-73 0.7501220229353756 1101 74-75 0.7738894206669364 1101 76-77 0.5020163627599601 1101 78-79 0.49904474022565637 1101 80-81 0.7841528769751314 1101 82-83 0.5837116306595789 1101 84-85 0.5911772632583379 1101 86-87 0.2199043493422117 1101 88-89 0.3051208313920455 1101 90-91 0.5874466081630771 1101 92-93 0.644318924716913 1101 94-95 0.38542197943786505 1101 96-97 0.4489716751767361 1101 98-99 0.5335516623012282 1101 100-101 1.2210947745917018 1102 1 0.3072463138291752 1102 2 0.3197929024867108 1102 3 0.29103672500479405 1102 4 0.25029443512022453 1102 5 0.17782777156655527 1102 6 0.1836006795854246 1102 7 0.16074334070237484 1102 8 0.30276403918842476 1102 9 0.42723668628216416 1102 10-11 0.39783498166213604 1102 12-13 0.3972007539587281 1102 14-15 0.5049465282950862 1102 16-17 0.4506572863374956 1102 18-19 0.27301053842412415 1102 20-21 0.34966391084484627 1102 22-23 0.3681747178811108 1102 24-25 0.42809458080262885 1102 26-27 0.4646945444871733 1102 28-29 0.37942541533762153 1102 30-31 0.36501465108931797 1102 32-33 0.5735677291247541 1102 34-35 0.6993944787352504 1102 36-37 0.5570520847143143 1102 38-39 0.5747932639226363 1102 40-41 0.423031443084092 1102 42-43 0.5828224802981694 1102 44-45 0.5914790704868338 1102 46-47 0.43755380765812646 1102 48-49 0.5503739014400608 1102 50-51 0.6314509968915587 1102 52-53 0.5057018773519033 1102 54-55 0.5294519094578405 1102 56-57 0.6488080091377952 1102 58-59 0.6324915496602657 1102 60-61 0.8103251176414119 1102 62-63 0.7390514319055157 1102 64-65 0.6320400054093227 1102 66-67 0.6828164599502387 1102 68-69 0.7161099308134382 1102 70-71 0.4934157779787114 1102 72-73 0.7170456921677619 1102 74-75 0.8742705429737292 1102 76-77 0.5969895132465268 1102 78-79 0.5624700715707718 1102 80-81 0.9152924226164316 1102 82-83 0.8339975083039448 1102 84-85 0.8115889270662286 1102 86-87 0.8730895147346409 1102 88-89 1.1135698805256489 1102 90-91 1.3190340766662558 1102 92-93 1.1498454136510077 1102 94-95 0.8645112508377437 1102 96-97 1.242585254795813 1102 98-99 1.327919441735851 1102 100-101 1.3773441460918576 1103 1 0.3160260769419949 1103 2 0.3371805948526827 1103 3 0.3057751992824862 1103 4 0.2264934160236649 1103 5 0.27848182265127264 1103 6 0.2871962016221019 1103 7 0.17083553565668552 1103 8 0.21358226602535524 1103 9 0.3321814423727929 1103 10-11 0.4126458426444799 1103 12-13 0.3716374230337749 1103 14-15 0.4495240199537065 1103 16-17 0.39233660376469714 1103 18-19 0.28395446105288613 1103 20-21 0.274585367887731 1103 22-23 0.36701044200523114 1103 24-25 0.30988709209359655 1103 26-27 0.4441056008459654 1103 28-29 0.36404357885309935 1103 30-31 0.4480685852728854 1103 32-33 0.4083264716797501 1103 34-35 0.40055104238278716 1103 36-37 0.3250148116500142 1103 38-39 0.2881784450788203 1103 40-41 0.44882275830315876 1103 42-43 0.6515533181167683 1103 44-45 0.5339965853633188 1103 46-47 0.4449920489453447 1103 48-49 0.5902650643087952 1103 50-51 0.5202704306245778 1103 52-53 0.6191927295666702 1103 54-55 0.5662230024023671 1103 56-57 0.6272076811651388 1103 58-59 0.6592746303842958 1103 60-61 0.601553743268191 1103 62-63 0.5042824063793958 1103 64-65 0.6021855635717088 1103 66-67 0.47779498748289484 1103 68-69 0.5582000258768005 1103 70-71 0.3977557330042387 1103 72-73 0.4757882471613648 1103 74-75 0.5774194322775408 1103 76-77 0.39760405607374594 1103 78-79 0.49202157166683946 1103 80-81 0.5868820617112043 1103 82-83 0.49608689979559983 1103 84-85 0.6165162291663897 1103 86-87 0.41892991761062603 1103 88-89 0.29117979330981925 1103 90-91 0.23524003916588399 1103 92-93 0.30855563140644904 1103 94-95 0.05719518748381702 1103 96-97 -0.02294812021061432 1103 98-99 0.06269455586330253 1103 100-101 0.6656610672620609 1104 1 0.26779747728978975 1104 2 0.26718378471099413 1104 3 0.22599015457301874 1104 4 0.09603721483621541 1104 5 0.2114446350773136 1104 6 0.13097289096077702 1104 7 0.16509764940086313 1104 8 0.17104653446736506 1104 9 0.27856738435853146 1104 10-11 0.29961231311771286 1104 12-13 0.2847742370856139 1104 14-15 0.29479145355835357 1104 16-17 0.42723403953021233 1104 18-19 0.3933241553409772 1104 20-21 0.3423222663164722 1104 22-23 0.3927048793039276 1104 24-25 0.3960640726354612 1104 26-27 0.2995902277869007 1104 28-29 0.24480526042291473 1104 30-31 0.34139869444835824 1104 32-33 0.2666363098201643 1104 34-35 0.21169856818462307 1104 36-37 0.2453255913131258 1104 38-39 0.3186878963202915 1104 40-41 0.2921621283575959 1104 42-43 0.3976296637739267 1104 44-45 0.17712025411196208 1104 46-47 0.27100650106144997 1104 48-49 -0.024722253431967545 1104 50-51 0.35702685921805966 1104 52-53 0.3614869065317734 1104 54-55 0.35484279701665145 1104 56-57 0.37038164163147513 1104 58-59 0.485121872185843 1104 60-61 0.7790487784080895 1104 62-63 0.5384338268953286 1104 64-65 0.37350809426122566 1104 66-67 0.3837134608784467 1104 68-69 0.42343237104004317 1104 70-71 0.3884562670482623 1104 72-73 0.3580786168762309 1104 74-75 0.2505715010550027 1104 76-77 0.18149244190254166 1104 78-79 0.32777216590656977 1104 80-81 0.6173323462259859 1104 82-83 0.5287958097901466 1104 84-85 0.4286271436267377 1104 86-87 0.2747348166155952 1104 88-89 0.2704283501561804 1104 90-91 0.4937645496255314 1104 92-93 0.4833167779032621 1104 94-95 0.38408363505180887 1104 96-97 0.48655457058922025 1104 98-99 0.32741999652679254 1104 100-101 0.709821954454263 1105 1 0.2548147826232636 1105 2 0.31529438515138253 1105 3 0.27000348455661083 1105 4 0.1887725954501036 1105 5 0.17734197055841605 1105 6 0.16354292756157918 1105 7 0.18731859905135195 1105 8 0.13751591146551334 1105 9 0.2851049137303079 1105 10-11 0.2055539710405796 1105 12-13 0.19644154414321235 1105 14-15 0.34784722668262447 1105 16-17 0.31051632631697146 1105 18-19 0.25743381489930073 1105 20-21 0.2825670188053735 1105 22-23 0.400565985899469 1105 24-25 0.21742794522722164 1105 26-27 0.40757891996083373 1105 28-29 0.43919254176636 1105 30-31 0.49675999808725635 1105 32-33 0.4089083922237009 1105 34-35 0.5488311438862254 1105 36-37 0.5390978510556863 1105 38-39 0.5300403034512939 1105 40-41 0.6313390289639926 1105 42-43 0.7014997801772935 1105 44-45 0.7591183107717825 1105 46-47 0.8055694465641849 1105 48-49 0.7722353354469149 1105 50-51 0.9895766988740107 1105 52-53 0.9030365787008776 1105 54-55 0.9829908726418992 1105 56-57 0.8490843327512252 1105 58-59 0.658287583192191 1105 60-61 0.7397275435373203 1105 62-63 0.6701619341460301 1105 64-65 0.5538036962533397 1105 66-67 0.3491255770749149 1105 68-69 0.36103797396811643 1105 70-71 0.21560367026302885 1105 72-73 0.2290158290081905 1105 74-75 0.5030379297918053 1105 76-77 0.36757095875714185 1105 78-79 0.48485267289914447 1105 80-81 0.60194202795169 1105 82-83 0.6139665724957482 1105 84-85 0.583630690407297 1105 86-87 0.3927634839620602 1105 88-89 0.380377623805078 1105 90-91 0.6305899224069442 1105 92-93 0.4806360645077632 1105 94-95 0.5719521677191359 1105 96-97 0.25272197863154133 1105 98-99 0.3456812981952595 1105 100-101 0.7177206224411208 1106 1 0.2370464774379144 1106 2 0.14288121225258976 1106 3 0.16439187283697976 1106 4 0.08918267476807529 1106 5 0.11627972123738317 1106 6 0.17077687061811275 1106 7 0.17743532776949422 1106 8 0.2165928732813711 1106 9 0.2389152340598102 1106 10-11 0.15086953427013583 1106 12-13 0.2860987322221362 1106 14-15 0.28622111829937325 1106 16-17 0.19704036451910412 1106 18-19 -0.03356692252403093 1106 20-21 0.07966858538031119 1106 22-23 0.16985949837270908 1106 24-25 0.23992115553675575 1106 26-27 0.15213367813992562 1106 28-29 0.18597755588532294 1106 30-31 0.28511320587876554 1106 32-33 0.2574191568057955 1106 34-35 0.20656599523246655 1106 36-37 0.34173908561027133 1106 38-39 0.5586622211116818 1106 40-41 0.6268236599825414 1106 42-43 0.37479786107802937 1106 44-45 0.29655873236209374 1106 46-47 0.3262433795485862 1106 48-49 0.446550520971833 1106 50-51 0.33582753072351323 1106 52-53 0.4942882199174434 1106 54-55 0.3021117201391519 1106 56-57 0.3904366952037819 1106 58-59 0.4638713167125843 1106 60-61 0.37448896010956645 1106 62-63 0.5237267464628061 1106 64-65 0.06431377736990385 1106 66-67 0.4151915135126245 1106 68-69 0.46281048786224943 1106 70-71 0.2504100516727661 1106 72-73 0.4704113577047515 1106 74-75 0.723668696532215 1106 76-77 0.6241466165927108 1106 78-79 0.7593576100383039 1106 80-81 0.6409634132284054 1106 82-83 0.8413618493892194 1106 84-85 0.7057749735778565 1106 86-87 0.5520190461124592 1106 88-89 0.6681256048838762 1106 90-91 0.5565472237972564 1106 92-93 0.8744471042746653 1106 94-95 0.9688118234683394 1106 96-97 0.7863817566851026 1106 98-99 0.5697896454676936 1106 100-101 0.6746290200741747 1107 1 0.015811477697660337 1107 2 0.04243502848029124 1107 3 -0.004270787988318148 1107 4 -0.023302389908366195 1107 5 -0.040698465975090414 1107 6 0.02614175428131915 1107 7 0.056882013714997015 1107 8 0.10910587494951329 1107 9 0.061534384752171434 1107 10-11 0.05691812385610007 1107 12-13 0.1367769546444606 1107 14-15 0.1790420618077775 1107 16-17 0.12379494035591421 1107 18-19 0.1397985614381767 1107 20-21 0.13586782692562593 1107 22-23 0.08698158418084034 1107 24-25 0.05502733342151345 1107 26-27 0.11537690890295238 1107 28-29 0.11984942668250653 1107 30-31 0.07797570854835811 1107 32-33 0.22128260032862812 1107 34-35 0.16551073886439127 1107 36-37 0.25370206332416956 1107 38-39 0.23167502941428353 1107 40-41 0.25636789451473163 1107 42-43 0.24078341926951197 1107 44-45 0.06318701452571673 1107 46-47 0.07338561000567978 1107 48-49 0.08116324153841958 1107 50-51 -0.11260216791485078 1107 52-53 -0.10806564479593561 1107 54-55 0.10044069553266866 1107 56-57 0.1191941917260877 1107 58-59 0.0645544454247613 1107 60-61 0.1317326641852432 1107 62-63 0.21311790475125747 1107 64-65 0.0905621395349101 1107 66-67 -0.08578454956494852 1107 68-69 0.19615161353830501 1107 70-71 0.24799096216472094 1107 72-73 0.021269092615021634 1107 74-75 -0.1082016966059065 1107 76-77 -0.052168413393374635 1107 78-79 0.22496971298958002 1107 80-81 0.12766072977645493 1107 82-83 -0.03259469819890626 1107 84-85 0.12562466794485871 1107 86-87 0.36819566100333745 1107 88-89 0.1873409448320409 1107 90-91 0.15187412700481673 1107 92-93 0.4861431220989303 1107 94-95 0.5723374256432692 1107 96-97 0.6190521636762796 1107 98-99 0.2013555600289827 1107 100-101 0.3730714173451197 1108 1 0.1793106414017558 1108 2 0.10083212431295152 1108 3 0.135518779192644 1108 4 0.15116252432986954 1108 5 0.185345578206082 1108 6 0.14567738358017834 1108 7 0.14904414243606823 1108 8 0.0743333098741985 1108 9 0.0015138584933680477 1108 10-11 0.09139986343996043 1108 12-13 0.13680319246182648 1108 14-15 0.1728651272244548 1108 16-17 0.22313242165801483 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sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 13.0 5 29.0 6 65.0 7 168.0 8 374.0 9 740.0 10 1355.0 11 2185.0 12 3330.0 13 4585.0 14 5958.0 15 8385.0 16 11118.0 17 15385.0 18 13544.0 19 8125.0 20 7093.0 21 7257.0 22 7870.0 23 8582.0 24 9885.0 25 11704.0 26 14240.0 27 17906.0 28 22477.0 29 28002.0 30 34445.0 31 43302.0 32 54556.0 33 70957.0 34 95113.0 35 133006.0 36 198212.0 37 282456.0 38 182376.0 39 10364.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.245584546559435 20.014770149998405 15.57087162138523 21.16877368205693 2 15.361628094422663 21.820793425330848 39.58184422876296 23.235734251483528 3 19.946622667117815 25.957047214608053 30.27642919329514 23.819900924978995 4 12.716114717775353 17.70638965052108 38.199668785043315 31.377826846660252 5 13.00566849026548 38.58603293122916 33.94714731611623 14.46115126238913 6 29.929149628259854 37.58476116322959 17.5627259220509 14.923363286459656 7 27.062743410337553 34.10588473242161 21.023961994151307 17.807409863089525 8 26.162020611846714 33.949858801094315 19.90129002726652 19.986830559792452 9 26.321184033942508 17.25365258351614 21.01021544825174 35.414947934289614 10-11 24.481448021608994 27.32101249574147 27.196374470725655 21.00116501192388 12-13 26.44095878391017 24.929666673256456 27.69262587384406 20.936748668989313 14-15 23.025344329156926 25.93645973207945 27.434445537673568 23.60375040109005 16-17 21.131134776902687 29.177039248277403 27.733267131297495 21.958558843522415 18-19 20.98363659796656 27.733078966407522 30.59584742698705 20.68743700863887 20-21 22.466528583768262 26.110518023772155 30.547875834884486 20.875077557575096 22-23 21.794300539409434 26.27560369906824 30.61437472151296 21.315721040009368 24-25 21.883885381947376 26.442972720380286 30.667310007292713 21.005831890379625 26-27 21.717298451884787 26.158729144852455 31.142483021984162 20.981489381278596 28-29 21.120211806606335 27.136314765785507 30.786853634761346 20.956619792846812 30-31 21.531070645380083 26.35636574883738 30.679696030512527 21.432867575270013 32-33 21.507723776609293 26.559830278420947 30.597181192233258 21.335264752736492 34-35 22.122340449798735 26.392599869522176 30.50481840315215 20.980241277526943 36-37 21.81397737974793 26.196019118512037 30.87774288711432 21.112260614625715 38-39 21.530111700002017 26.694501996394663 30.733413156166694 21.041973147436625 40-41 21.76824608693338 26.637062833375786 30.46302879150208 21.131662288188753 42-43 21.429081956194125 26.939336935413476 29.831975583310395 21.799605525082004 44-45 21.819488946642476 26.567406699990727 29.82353558410117 21.789568769265628 46-47 22.231899948447413 27.08346956496614 29.300192675883274 21.38443781070317 48-49 22.162882271557137 27.129822437649015 29.065888129217548 21.641407161576304 50-51 21.624737275943147 27.41728944815021 29.303237551214085 21.654735724692564 52-53 22.137281529479928 27.911457565052622 28.34068094826052 21.610579957206923 54-55 21.837370468488142 27.318944829536985 28.6747105215934 22.168974180381472 56-57 22.204796497315236 27.456048202976874 28.359359762391684 21.979795537316207 58-59 22.663663235531732 27.158925227871077 28.446598804330264 21.730812732266923 60-61 22.221947641096783 27.382788604511553 29.132305479021348 21.262958275370313 62-63 21.971739415587997 27.27239545807962 29.080380888651476 21.675484237680905 64-65 21.77670549957819 27.712084685984152 28.790999148775686 21.720210665661977 66-67 22.002848383280156 27.435382912133534 28.290354124148752 22.271414580437554 68-69 21.814819630448287 27.355358013201425 28.704014185335485 22.125808171014803 70-71 22.336476063784286 27.310389715557488 28.451322908425382 21.901811312232848 72-73 22.115991441384587 27.04791638469973 28.830238920410046 22.00585325350564 74-75 21.78509920808416 28.172738781825856 28.038915269186752 22.003246740903233 76-77 21.997071092166312 27.41889616976108 28.238374471359506 22.3456582667131 78-79 21.98777774567309 27.59116506050297 28.271928050355015 22.14912914346892 80-81 22.296823603302467 27.7066741284945 27.977824380407228 22.018677887795807 82-83 22.130601204469748 27.50299710612436 28.357205621061603 22.009196068344288 84-85 21.87649827896343 27.765549688015785 28.103179307562716 22.25477272545807 86-87 21.848880710505505 27.73229819332076 28.08363495346432 22.33518614270941 88-89 21.981259364329986 27.58075326755951 27.739744906241697 22.69824246186881 90-91 21.9514225851865 27.283289428612594 28.031944438636103 22.7333435475648 92-93 22.21517620616136 27.58036260116609 28.046083986483815 22.158377206188735 94-95 21.905827857471984 27.456229859956842 28.029123319793854 22.60881896277732 96-97 21.412755441497957 27.55384469156924 28.395323638437407 22.63807622849539 98-99 21.64563521498008 27.707149122123344 27.674566878571643 22.972648784324935 100-101 21.476984912090476 27.46717317069647 27.859597204596554 23.196244712616497 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 751.0 1 516.5 2 302.0 3 336.5 4 311.5 5 317.0 6 532.0 7 1126.5 8 1959.5 9 2405.0 10 2090.5 11 1735.5 12 1768.5 13 1877.0 14 2051.5 15 2221.5 16 2348.0 17 2502.0 18 2678.5 19 2849.5 20 3093.0 21 3517.0 22 4086.5 23 4819.5 24 5636.0 25 6694.5 26 8353.5 27 10387.0 28 12037.5 29 13852.0 30 16471.0 31 18871.5 32 21228.0 33 23993.0 34 27086.0 35 29908.5 36 33365.5 37 36972.0 38 38784.5 39 39674.5 40 40201.0 41 41150.0 42 41961.5 43 42932.5 44 44864.5 45 46263.0 46 46758.5 47 51699.0 48 64823.0 49 64309.5 50 50579.0 51 44278.5 52 42184.5 53 40612.5 54 38903.0 55 35897.5 56 32183.0 57 28827.0 58 26007.0 59 23157.5 60 19550.0 61 15719.5 62 12075.0 63 9011.0 64 6465.0 65 4580.0 66 3264.5 67 2410.5 68 1752.0 69 1182.5 70 782.5 71 512.0 72 360.5 73 282.5 74 194.0 75 119.0 76 80.5 77 47.0 78 24.0 79 7.5 80 5.0 81 3.5 82 3.5 83 3.0 84 1.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026764682177002754 2 0.0044100896768924985 3 7.603602891193963E-5 4 6.08288231295517E-4 5 7.603602891193963E-5 6 0.0 7 0.0 8 0.0 9 7.603602891193963E-5 10-11 0.012013692568086463 12-13 0.0 14-15 0.0 16-17 0.0 18-19 4.1819815901566803E-4 20-21 0.0010645044047671549 22-23 0.0018248646938865511 24-25 0.011785584481350643 26-27 0.006310990399690989 28-29 3.041441156477585E-4 30-31 0.0025852249830059476 32-33 0.0053985580527477145 34-35 0.0 36-37 0.008021801050209632 38-39 0.012964142929485707 40-41 0.0015967566071507324 42-43 0.0 44-45 0.0 46-47 3.041441156477585E-4 48-49 0.003839819460052951 50-51 0.007451530833370084 52-53 0.0 54-55 0.003193513214301465 56-57 0.004600179749172348 58-59 2.661261011917887E-4 60-61 0.0023951349107260984 62-63 0.0063490084141469595 64-65 1.1405404336790945E-4 66-67 0.0025852249830059476 68-69 3.801801445596982E-4 70-71 0.0011025224192231246 72-73 0.0 74-75 7.603602891193963E-5 76-77 0.0 78-79 0.0018248646938865511 80-81 0.0021290088095343098 82-83 0.011215314264511096 84-85 0.008782161339329027 86-87 0.003497657329949223 88-89 1.900900722798491E-4 90-91 5.702702168395473E-4 92-93 1.5207205782387926E-4 94-95 0.0 96-97 0.003117477185389525 98-99 0.002471170939638038 100-101 5.322522023835774E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1315166.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.77574244278132 #Duplication Level Percentage of deduplicated Percentage of total 1 80.02816539141931 48.63771167997206 2 12.093380837504572 14.699683980852898 3 3.5002606144297315 6.381928127555786 4 1.4640010818391214 3.5590301074323065 5 0.7841858722206898 2.3829739298676236 6 0.46886498176875624 1.7097370423450373 7 0.3098858061579699 1.3183477959211307 8 0.22144529846641836 1.0766801939807904 9 0.15985887179264607 0.8743987457337115 >10 0.8853069126333366 9.809461101551703 >50 0.05622623156394616 2.356925170797058 >100 0.024161674078145554 2.544356336060632 >500 0.00200302405898656 0.8297019790076811 >1k 0.0018778350552998999 2.2045497333101842 >5k 3.7556701105997996E-4 1.6145140756114618 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7836 0.595818322553959 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7096 0.5395516611591237 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6288 0.4781145497982764 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4053 0.30817402518009135 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3316 0.2521354718719918 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2795 0.21252070080887128 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2189 0.16644286728823585 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2110 0.16043602100419263 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2028 0.15420106663341357 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1852 0.1408187255449122 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1618 0.12302629477951832 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1546 0.11755170069785868 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1486 0.11298953896314229 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1397 0.10622233238997966 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1331 0.10120395448179165 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 7.603602891193963E-5 0.0 0.0 0.0 0.0 7 7.603602891193963E-5 0.0 0.0 0.0 0.0 8 7.603602891193963E-5 0.0 0.0 0.0 0.0 9 7.603602891193963E-5 0.0 0.0 0.0 0.0 10-11 7.603602891193963E-5 0.0 0.0 7.603602891193963E-5 0.0 12-13 7.603602891193963E-5 0.0 0.0 7.603602891193963E-5 0.0 14-15 7.603602891193963E-5 0.0 0.0 1.5207205782387926E-4 0.0 16-17 7.603602891193963E-5 0.0 0.0 1.5207205782387926E-4 0.0 18-19 7.603602891193963E-5 0.0 0.0 1.5207205782387926E-4 0.0 20-21 7.603602891193963E-5 7.603602891193963E-5 0.0 1.9009007227984906E-4 0.0 22-23 7.603602891193963E-5 7.603602891193963E-5 0.0 3.801801445596982E-4 0.0 24-25 7.603602891193963E-5 7.603602891193963E-5 0.0 6.843242602074568E-4 0.0 26-27 7.603602891193963E-5 7.603602891193963E-5 0.0 9.504503613992454E-4 0.0 28-29 7.603602891193963E-5 7.603602891193963E-5 0.0 0.001862882708342521 0.0 30-31 7.603602891193963E-5 1.5207205782387926E-4 0.0 0.00410594556124474 0.0 32-33 7.603602891193963E-5 1.9009007227984906E-4 0.0 0.006691170544250688 0.0 34-35 1.1405404336790945E-4 2.281080867358189E-4 0.0 0.009656575671816332 0.0 36-37 3.801801445596982E-4 2.281080867358189E-4 0.0 0.01227981866927825 0.0 38-39 3.801801445596982E-4 2.281080867358189E-4 0.0 0.01425675542098868 0.0 40-41 3.801801445596982E-4 2.281080867358189E-4 0.0 0.016803962389538657 0.0 42-43 4.5621617347163784E-4 2.281080867358189E-4 0.0 0.02075783589295952 0.0 44-45 6.08288231295517E-4 2.281080867358189E-4 0.0 0.023723241020525164 0.0 46-47 7.223422746634265E-4 2.281080867358189E-4 0.0 0.027981258639593783 0.0 48-49 7.603602891193964E-4 2.281080867358189E-4 0.0 0.0337599968369012 0.0 50-51 7.603602891193964E-4 2.281080867358189E-4 0.0 0.037789906369233996 0.0 52-53 7.603602891193964E-4 3.421621301037284E-4 0.0 0.047180355939858545 0.0 54-55 7.983783035753662E-4 3.801801445596982E-4 0.0 0.06668359735577106 0.0 56-57 9.124323469432756E-4 3.801801445596982E-4 0.0 0.10428341365272521 0.0 58-59 9.124323469432756E-4 3.801801445596982E-4 0.0 0.14617926558320393 0.0 60-61 9.124323469432756E-4 3.801801445596982E-4 0.0 0.18385511790907003 0.0 62-63 9.884683758552152E-4 3.801801445596982E-4 0.0 0.23213799626815168 0.0 64-65 9.884683758552152E-4 3.801801445596982E-4 0.0 0.29353708961454295 0.0 66-67 0.0011405404336790946 3.801801445596982E-4 0.0 0.385464648569078 0.0 68-69 0.0011405404336790946 4.18198159015668E-4 0.0 0.49788391731538073 0.0 70-71 0.001216576462591034 4.562161734716378E-4 0.0 0.5968448089442702 0.0 72-73 0.0012926124915029738 4.562161734716378E-4 0.0 0.6963379527755431 0.0 74-75 0.0012926124915029738 4.562161734716378E-4 0.0 0.8020660509775952 0.0 76-77 0.0013306305059589438 4.562161734716378E-4 0.0 0.9261188321474247 0.0 78-79 0.0013686485204149135 4.562161734716378E-4 0.0 1.0304782818290619 0.0 80-81 0.0015207205782387927 4.562161734716378E-4 0.0 1.136206380031114 0.0 82-83 0.0015207205782387927 4.562161734716378E-4 0.0 1.254670513075916 0.0 84-85 0.0015967566071507324 4.562161734716378E-4 0.0 1.4451027474858686 0.0 86-87 0.0017108106505186417 4.562161734716378E-4 0.0 1.6848823646596705 0.0 88-89 0.0017488286649746116 4.562161734716378E-4 0.0 1.9629081043761776 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATACCG 70 0.008383042 27.148802 5 TCCCGTA 90 8.956666E-4 26.384632 2 CCCGTAC 95 0.0012278027 24.995968 3 GAGTACT 6870 0.0 24.16095 12-13 GACCGTA 100 0.001651666 23.755201 7 GTACTTT 7105 0.0 23.027601 14-15 AGTACTT 6980 0.0 22.317316 12-13 GTACATG 7290 0.0 21.048962 1 TACATGG 7305 0.0 20.08916 2 AGAGTAC 11905 0.0 19.734476 10-11 ACATGGG 7270 0.0 19.401958 3 ACTTTTT 8610 0.0 19.251402 16-17 TACTTTT 8265 0.0 18.73261 14-15 GTATCAA 17665 0.0 18.529396 1 AGTACAT 5620 0.0 17.49273 2 CTTACAC 605 0.0 17.269941 3 CAACGCA 19765 0.0 16.489822 5 GTATTAC 260 6.6153225E-6 16.444658 1 TCAACGC 19760 0.0 16.391586 4 ATCAACG 19820 0.0 16.318003 3 >>END_MODULE