FastQCFastQC Report
Thu 26 May 2016
SRR936884_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936884_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297651
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67750.5220972356974256No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT60950.46969485632115265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49970.3850804260929942No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT38600.2974605652829613No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32090.24729299326244114No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT30330.2337300244826999No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC23000.1772433420079821No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT20920.16121437890465157No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT17390.13401137902255691No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC17210.13262425721553794No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC15560.1199089739845305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG72800.024.027431
TACATGG74300.022.648472
GAGTACT56300.022.413712-13
AGTACTT58950.021.76894412-13
ACATGGG74750.021.4318623
GTACTTT59100.021.35056114-15
CCTATAC3151.4551915E-1121.1280123
CATGGGA50050.019.7560634
TACTTTT67200.019.37820414-15
AGAGTAC110650.019.37086510-11
GTATCAA161950.019.1949831
ACTTTTT70300.018.45359416-17
GTGTAGC7400.017.9852811
TTGCGTT2403.0672036E-617.8267614
TTTGCGT2403.0672036E-617.8267613
CGTTTAG1203.0888768E-617.81507590-91
ATGGGAG21600.017.3865935
CAACGCA182700.017.0429175
TCAACGC184500.016.902414
TATCAAC184000.016.8960172
Produced by FastQC (version 0.11.5)