##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936884_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1297651 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.573751339921134 31.0 31.0 34.0 30.0 34.0 2 31.629651578120775 33.0 31.0 34.0 30.0 34.0 3 31.644722656554034 33.0 31.0 34.0 28.0 34.0 4 35.25612125294089 37.0 35.0 37.0 33.0 37.0 5 35.27039781882802 37.0 35.0 37.0 33.0 37.0 6 35.35024363253294 37.0 35.0 37.0 33.0 37.0 7 35.29115917916297 37.0 35.0 37.0 33.0 37.0 8 35.26430604222553 37.0 35.0 37.0 32.0 37.0 9 36.84472019055971 39.0 37.0 39.0 33.0 39.0 10-11 36.70056432738849 39.0 37.0 39.0 32.0 39.0 12-13 36.60681415881466 39.0 37.0 39.0 32.0 39.0 14-15 37.66139932847892 40.0 37.0 41.0 32.0 41.0 16-17 37.60972942647908 40.0 37.0 41.0 32.0 41.0 18-19 37.59934450788386 40.0 37.0 41.0 32.0 41.0 20-21 37.53813660221431 40.0 37.0 41.0 32.0 41.0 22-23 37.42259860316834 40.0 37.0 41.0 32.0 41.0 24-25 37.3662055514156 40.0 37.0 41.0 31.5 41.0 26-27 37.37830780387023 40.0 37.0 41.0 31.5 41.0 28-29 37.31946224370034 40.0 37.0 41.0 31.0 41.0 30-31 37.15747839750441 39.5 36.5 41.0 31.0 41.0 32-33 36.951176394885834 39.0 36.0 41.0 30.5 41.0 34-35 36.77506625433186 39.0 36.0 41.0 30.0 41.0 36-37 36.58306123911591 39.0 35.0 41.0 30.0 41.0 38-39 36.40938164421713 39.0 35.0 41.0 30.0 41.0 40-41 36.235318664263346 39.0 35.0 41.0 30.0 41.0 42-43 35.99993449702578 39.0 35.0 40.0 29.0 41.0 44-45 35.70500080530127 39.0 35.0 40.0 27.5 41.0 46-47 35.32460653904633 38.0 35.0 40.0 26.5 41.0 48-49 35.02972409376635 38.5 34.5 40.0 25.0 41.0 50-51 34.305716637216015 38.0 33.5 39.5 24.0 40.5 52-53 34.49732863458665 38.0 34.0 39.5 24.0 40.5 54-55 34.89380002789656 38.5 34.0 40.0 25.0 41.0 56-57 34.75741859714206 38.0 34.0 40.0 24.5 41.0 58-59 34.54743031832133 38.0 34.0 40.0 24.0 41.0 60-61 34.23079510592601 38.0 34.0 40.0 23.0 41.0 62-63 33.92674995048746 37.5 34.0 40.0 22.0 41.0 64-65 33.59768150296189 37.0 33.0 40.0 22.0 41.0 66-67 33.20534912699948 36.5 33.0 39.0 21.0 41.0 68-69 32.80093915852567 36.0 32.0 39.0 20.0 41.0 70-71 32.37231890546842 35.5 32.0 39.0 18.5 40.0 72-73 31.97021310044072 35.0 31.5 38.0 17.5 40.0 74-75 31.577632198487883 35.0 31.0 37.0 14.5 39.5 76-77 31.125282144428663 35.0 31.0 37.0 10.0 39.0 78-79 30.654566982956126 35.0 30.5 36.0 7.5 39.0 80-81 30.209233453370743 34.5 30.0 36.0 6.5 37.5 82-83 29.80619789142073 34.0 30.0 35.0 2.0 37.0 84-85 29.425253400182328 34.0 29.0 35.0 2.0 37.0 86-87 29.102732552897507 34.0 29.0 35.0 2.0 36.0 88-89 28.716235336003287 34.0 29.0 35.0 2.0 36.0 90-91 28.33747055255997 34.0 29.0 35.0 2.0 35.5 92-93 27.94534971267313 34.0 27.5 35.0 2.0 35.0 94-95 27.5484028448327 34.0 27.0 35.0 2.0 35.0 96-97 27.03211687888346 33.5 26.0 35.0 2.0 35.0 98-99 26.44256506564554 33.0 25.0 35.0 2.0 35.0 100-101 24.220148560745532 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.47388575992030724 1101 2 0.3931327694204363 1101 3 0.46212316451068247 1101 4 0.39722970493765075 1101 5 0.38634997301652874 1101 6 0.384577449569548 1101 7 0.4828198381344677 1101 8 0.4237865099238931 1101 9 0.39665787318251233 1101 10-11 0.5692412218189773 1101 12-13 0.6280405716482349 1101 14-15 0.8392343082379199 1101 16-17 0.740161200264879 1101 18-19 0.8672053651704132 1101 20-21 0.9143865479788786 1101 22-23 0.9326416469272587 1101 24-25 0.87261399113099 1101 26-27 0.777779255130234 1101 28-29 0.7521228559638686 1101 30-31 0.6782866515806845 1101 32-33 0.6145462159480815 1101 34-35 0.7179975268516685 1101 36-37 0.7186581088168751 1101 38-39 0.6384526403221145 1101 40-41 0.6649514127598408 1101 42-43 0.6491290324965249 1101 44-45 0.424914472723664 1101 46-47 0.4366936646886401 1101 48-49 0.21511275331462798 1101 50-51 0.5742170348163427 1101 52-53 0.5811801155720886 1101 54-55 0.5275240184179495 1101 56-57 0.46502803849489993 1101 58-59 0.5589557997633463 1101 60-61 0.5724604562298126 1101 62-63 0.34579832953943423 1101 64-65 0.4408019101092222 1101 66-67 0.3660127557383106 1101 68-69 0.34192698718795356 1101 70-71 0.21471522338912052 1101 72-73 0.20171733071646258 1101 74-75 -0.007525833496558221 1101 76-77 0.3847007238144933 1101 78-79 0.34221893925084146 1101 80-81 0.2822122461022616 1101 82-83 0.4690741374301908 1101 84-85 0.395286733533613 1101 86-87 0.43995481785931645 1101 88-89 0.5527387730013658 1101 90-91 0.6550894094053668 1101 92-93 0.5834073428451063 1101 94-95 0.7009722654889501 1101 96-97 0.8532852671023043 1101 98-99 0.7025928031846327 1101 100-101 0.9099942260873952 1102 1 0.3705288337594794 1102 2 0.37500571840712027 1102 3 0.22760309489073904 1102 4 0.30224724463771935 1102 5 0.3439307068280044 1102 6 0.33304548744020934 1102 7 0.31012923197751974 1102 8 0.3136204889840215 1102 9 0.36866238339109714 1102 10-11 0.3948242619258835 1102 12-13 0.31301065177583354 1102 14-15 0.23519438453966046 1102 16-17 0.504740907559345 1102 18-19 0.35111585612035157 1102 20-21 0.36798952838076104 1102 22-23 0.39941826227705945 1102 24-25 0.35064659449667346 1102 26-27 0.48011525489314266 1102 28-29 0.4363311311458702 1102 30-31 0.5024444226646949 1102 32-33 0.3622914484366717 1102 34-35 0.3104126842778854 1102 36-37 0.11513683216379889 1102 38-39 0.2357821468934418 1102 40-41 0.30781341492603786 1102 42-43 0.33252060697424213 1102 44-45 0.2082846951087376 1102 46-47 0.025119954840000958 1102 48-49 -0.011987969288689726 1102 50-51 -0.14369892011390561 1102 52-53 0.0931201114768001 1102 54-55 -0.09836205974822576 1102 56-57 0.023150185936152923 1102 58-59 -0.30788830705908765 1102 60-61 -0.0504902143740793 1102 62-63 -0.20499775999656578 1102 64-65 -0.09127438893323614 1102 66-67 -0.43665450456592225 1102 68-69 -0.15293908063222972 1102 70-71 -0.2346991769471316 1102 72-73 -0.14605134192429858 1102 74-75 -0.3436313465125771 1102 76-77 -0.30570875139552456 1102 78-79 -0.2812645233176596 1102 80-81 -0.05477463010679173 1102 82-83 -0.025295511075295707 1102 84-85 -0.0821689591902377 1102 86-87 0.030219273966960003 1102 88-89 0.12407463266221441 1102 90-91 0.05150549096631707 1102 92-93 0.14495626540638895 1102 94-95 0.294659118527278 1102 96-97 -0.013935364003582862 1102 98-99 0.10457827837310063 1102 100-101 0.1239051481205955 1103 1 0.1717113669843222 1103 2 0.14773087356275738 1103 3 0.21420211529629896 1103 4 0.22560913618034562 1103 5 0.20039555151282684 1103 6 0.13366068551292898 1103 7 0.22258220665803918 1103 8 0.11717957575949356 1103 9 -0.028635595198217345 1103 10-11 -0.004242092036371048 1103 12-13 0.02255005093663698 1103 14-15 0.35675216306196944 1103 16-17 0.1884072072769527 1103 18-19 0.22228068006457846 1103 20-21 0.13322988001368685 1103 22-23 0.1828484382530675 1103 24-25 0.20402906709514923 1103 26-27 0.14984100035812986 1103 28-29 0.20339314553665844 1103 30-31 0.322847747542248 1103 32-33 0.26524969601560144 1103 34-35 0.4162324963361499 1103 36-37 0.3833919096219631 1103 38-39 0.2765629506714191 1103 40-41 0.250541920486107 1103 42-43 0.4221266951377416 1103 44-45 0.4117885799824563 1103 46-47 0.21442610203443735 1103 48-49 0.584369343367861 1103 50-51 0.28570710104150976 1103 52-53 0.4014667666563625 1103 54-55 0.46069535980218745 1103 56-57 0.5270057037331739 1103 58-59 0.5613817834018349 1103 60-61 0.7152941791229424 1103 62-63 0.7534658402523249 1103 64-65 0.5323702129270416 1103 66-67 0.49971844331135173 1103 68-69 0.4737007827989075 1103 70-71 0.396241648528715 1103 72-73 0.37098658146311436 1103 74-75 0.3875092268852107 1103 76-77 0.3753305121536705 1103 78-79 0.3279892937502602 1103 80-81 0.36432203703850163 1103 82-83 0.4402084057070397 1103 84-85 0.3795394279888811 1103 86-87 0.5730508443493854 1103 88-89 0.3327390976345299 1103 90-91 0.45055493336329633 1103 92-93 0.5456528304441228 1103 94-95 0.7946961325192063 1103 96-97 0.8223590886839212 1103 98-99 0.7199668455167973 1103 100-101 0.8576718004283883 1104 1 0.46950647878879437 1104 2 0.36350708108164653 1104 3 0.3904842724649349 1104 4 0.3945822480459924 1104 5 0.48322752361524124 1104 6 0.3315120819217299 1104 7 0.3219655231671652 1104 8 0.3826618185786188 1104 9 0.3505959975949011 1104 10-11 0.5972099895763066 1104 12-13 0.4351978744966374 1104 14-15 0.2980592506395965 1104 16-17 0.6839234043954292 1104 18-19 0.4384193726711061 1104 20-21 0.6469798417768544 1104 22-23 0.5273398884993057 1104 24-25 0.5609043538713294 1104 26-27 0.4975678976693203 1104 28-29 0.5616654528995397 1104 30-31 0.7968087765716447 1104 32-33 0.5499127531261223 1104 34-35 0.4845932072351715 1104 36-37 0.48565736494606426 1104 38-39 0.7602388859441263 1104 40-41 0.5169953786244363 1104 42-43 0.427237276031569 1104 44-45 0.48642135352869076 1104 46-47 0.4066631091617765 1104 48-49 0.47975125276188635 1104 50-51 0.32243096999626175 1104 52-53 0.4845266302273714 1104 54-55 0.6289713379001327 1104 56-57 0.5072788676009097 1104 58-59 0.48639417213267677 1104 60-61 0.6346634244809977 1104 62-63 0.3045606369853715 1104 64-65 0.3610637371385934 1104 66-67 0.2320934088982085 1104 68-69 0.22670191460888844 1104 70-71 0.3752737980499887 1104 72-73 0.4080478521492381 1104 74-75 0.43902524046692903 1104 76-77 0.3622477064884997 1104 78-79 0.37467094925177236 1104 80-81 0.1101927659853068 1104 82-83 0.103501666849251 1104 84-85 0.3214368921227404 1104 86-87 0.4735032624461084 1104 88-89 0.37779675876450725 1104 90-91 0.31495706238963095 1104 92-93 0.34763839221953674 1104 94-95 0.5320005894033599 1104 96-97 0.6340687335402997 1104 98-99 0.671650489333139 1104 100-101 0.5840096304121758 1105 1 0.1392209455283826 1105 2 0.10479195086724147 1105 3 0.2550134743226664 1105 4 0.25130217201536453 1105 5 0.25203404599108126 1105 6 0.15865955862539494 1105 7 0.17630769685411707 1105 8 0.1889307589914111 1105 9 0.28939911293790743 1105 10-11 0.4151719264445859 1105 12-13 0.40130596709619937 1105 14-15 0.4643789379098422 1105 16-17 0.4480736485661154 1105 18-19 0.38141523043645265 1105 20-21 0.4563085367637427 1105 22-23 0.43863838641623687 1105 24-25 0.332318451814551 1105 26-27 0.4794060217689875 1105 28-29 0.44873008491010324 1105 30-31 0.40561087578205246 1105 32-33 0.33176761352743256 1105 34-35 0.01214414835327915 1105 36-37 0.19676130335646747 1105 38-39 0.41670268472596206 1105 40-41 0.28916261014128253 1105 42-43 0.439647542557168 1105 44-45 0.40947620675730434 1105 46-47 0.33456132853883247 1105 48-49 0.3746690954526031 1105 50-51 0.2536764497005066 1105 52-53 0.5740428315650874 1105 54-55 0.29500232396715376 1105 56-57 0.4582926093000026 1105 58-59 0.4167705596019644 1105 60-61 0.5415055832248115 1105 62-63 0.6045994891141717 1105 64-65 0.618090294062938 1105 66-67 0.708765096455366 1105 68-69 0.6161664127290436 1105 70-71 0.6097147159165814 1105 72-73 0.4357240166670806 1105 74-75 0.7134321477777057 1105 76-77 0.6948527118381413 1105 78-79 0.6337228975366003 1105 80-81 0.4660394136443209 1105 82-83 0.3497058138657039 1105 84-85 0.5412117290932308 1105 86-87 0.5954263232765911 1105 88-89 0.6093584350246601 1105 90-91 0.6663629117270524 1105 92-93 0.7687359947443255 1105 94-95 0.6860731890889582 1105 96-97 0.7581941123485585 1105 98-99 0.8069129802925481 1105 100-101 0.7512079735183157 1106 1 0.40819076773338736 1106 2 0.3943339642372301 1106 3 0.4147503795458185 1106 4 0.32110531217882965 1106 5 0.3024218002145602 1106 6 0.4008334422560793 1106 7 0.5041835663030199 1106 8 0.4903624668934796 1106 9 0.5661738341915097 1106 10-11 0.488903345914089 1106 12-13 0.5920386104053676 1106 14-15 0.534129496017627 1106 16-17 0.5421906082777994 1106 18-19 0.6504388646867412 1106 20-21 0.5535238402944174 1106 22-23 0.6576245059835273 1106 24-25 0.5142320696444855 1106 26-27 0.4571339849677045 1106 28-29 0.4889528975273336 1106 30-31 0.7666767847751217 1106 32-33 0.7772649290751161 1106 34-35 0.722525260632878 1106 36-37 0.6234142556390196 1106 38-39 0.5260924253826573 1106 40-41 0.7731047883298814 1106 42-43 0.5731813641963797 1106 44-45 0.49196545972311156 1106 46-47 0.7282431794271886 1106 48-49 0.8621142840407643 1106 50-51 0.8561244365150245 1106 52-53 0.8929825190247271 1106 54-55 0.9550012169765125 1106 56-57 0.8431492847610969 1106 58-59 0.8504083153629338 1106 60-61 1.0225849606040782 1106 62-63 0.9028464464273824 1106 64-65 0.7454901575340926 1106 66-67 0.7164420765848831 1106 68-69 0.7287941010250805 1106 70-71 0.8486657192806035 1106 72-73 0.8747857190956978 1106 74-75 1.223583252000875 1106 76-77 1.2413466264645656 1106 78-79 1.1887975271559803 1106 80-81 1.0964382992737356 1106 82-83 1.1764596252959976 1106 84-85 1.1293257245299664 1106 86-87 1.3632916148148766 1106 88-89 1.3471677005363674 1106 90-91 1.175360900694045 1106 92-93 1.1081706915211385 1106 94-95 1.333964064411063 1106 96-97 1.156543773793917 1106 98-99 1.412102306442744 1106 100-101 1.5293310708781434 1107 1 0.3133981139722728 1107 2 0.3292853266693001 1107 3 0.28226731728070575 1107 4 0.1761879219115059 1107 5 0.04185078420831445 1107 6 0.28255744903761837 1107 7 0.3562273755689773 1107 8 0.1793199017694178 1107 9 0.20346457016913178 1107 10-11 0.22078980023861305 1107 12-13 0.352046861709141 1107 14-15 0.32506305368328015 1107 16-17 0.5527729053634189 1107 18-19 0.6181275971446638 1107 20-21 0.6508060671150275 1107 22-23 0.40790395443544014 1107 24-25 0.3354205152406706 1107 26-27 0.5831081867507564 1107 28-29 0.44904398418537284 1107 30-31 0.48830487015032276 1107 32-33 0.3220321198285063 1107 34-35 0.4108793189206068 1107 36-37 0.41114527602876905 1107 38-39 0.5236453981082292 1107 40-41 0.6020278642612098 1107 42-43 0.45918315627641704 1107 44-45 0.6471441197887415 1107 46-47 0.44352130638123555 1107 48-49 0.5800792417850573 1107 50-51 0.3662693500374914 1107 52-53 0.2844292046436081 1107 54-55 0.3263414736694159 1107 56-57 0.5114800717936561 1107 58-59 0.6414762790314086 1107 60-61 0.5480605386788184 1107 62-63 0.5307916139352926 1107 64-65 0.3943262724592813 1107 66-67 0.47617958198368626 1107 68-69 0.47617656829383037 1107 70-71 0.5873401602225385 1107 72-73 0.48304894857864156 1107 74-75 0.5587305828974927 1107 76-77 0.5733413791510706 1107 78-79 0.5155536688979083 1107 80-81 0.6914375579456618 1107 82-83 0.7752332108098017 1107 84-85 0.6594732810481752 1107 86-87 0.7736934146305074 1107 88-89 0.8154794708922708 1107 90-91 0.7468365303394329 1107 92-93 0.7732644534065614 1107 94-95 0.6410840370786168 1107 96-97 0.569937638176004 1107 98-99 1.0754168809526625 1107 100-101 1.1363346872698727 1108 1 0.07773931109609933 1108 2 0.09620510157731488 1108 3 0.21469583374176082 1108 4 0.14295679380627035 1108 5 0.05615109924089978 1108 6 0.24127134654381166 1108 7 0.12955120982816482 1108 8 0.10587101640761176 1108 9 -0.05315772740439684 1108 10-11 -0.05554067134833218 1108 12-13 0.10968489888779231 1108 14-15 0.3398142741339427 1108 16-17 0.2579492995629735 1108 18-19 0.1623980922989361 1108 20-21 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2316 64-65 -2.750465872141689 2316 66-67 -2.438839592032547 2316 68-69 -2.406242246599028 2316 70-71 -2.343678979807251 2316 72-73 -2.5522156641742058 2316 74-75 -2.8789903997637545 2316 76-77 -3.1062248405939172 2316 78-79 -3.27034224196699 2316 80-81 -3.174112654186363 2316 82-83 -3.9521550446096185 2316 84-85 -3.6434512520631124 2316 86-87 -4.090827858048357 2316 88-89 -3.855781879162393 2316 90-91 -4.514195751113601 2316 92-93 -4.48927512663434 2316 94-95 -4.900289071717637 2316 96-97 -5.072061387546064 2316 98-99 -5.275967500012666 2316 100-101 -5.410199545571221 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2701.0 3 950.0 4 1026.0 5 1081.0 6 1224.0 7 1473.0 8 1833.0 9 2314.0 10 3106.0 11 3847.0 12 4922.0 13 6329.0 14 7021.0 15 8645.0 16 11149.0 17 14315.0 18 12613.0 19 8020.0 20 7930.0 21 8422.0 22 9076.0 23 10222.0 24 11613.0 25 13219.0 26 15399.0 27 18446.0 28 21482.0 29 26236.0 30 32189.0 31 39211.0 32 49173.0 33 63886.0 34 84550.0 35 118413.0 36 172182.0 37 246688.0 38 219214.0 39 37511.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.165135125134135 19.458080988738544 14.898070257669014 21.478713628458305 2 14.941617024950357 21.257060840394175 40.43572168572169 23.365600448933783 3 19.719640864047555 26.109118994616658 29.947677394291073 24.223562747044713 4 12.293401013663017 17.13227731871175 38.78995711900132 31.784364548623905 5 13.046798407630783 38.52030918682261 33.87440770174779 14.558484703798808 6 29.781405166534487 37.30562233256287 18.074692868627082 14.838279632275563 7 26.998233524785014 34.102918192381566 21.20194335405144 17.696904928781983 8 25.89707715280006 33.864180467458105 20.212580521756298 20.026161857985535 9 26.280684923900772 17.504287714925567 21.169204335836042 35.04582302533762 10-11 24.314972094526176 27.26450008830114 27.386425068577978 21.0341027485947 12-13 26.243187124412216 24.958982970461953 27.666396111720555 21.13143379340527 14-15 23.071901876066562 25.886194497416348 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21.18975122514737 40-41 21.776183985859333 27.022210199420517 29.889224525436507 21.31238128928364 42-43 21.431571500352963 27.145827489156805 29.24456761314069 22.178033397349544 44-45 21.747584113390133 26.914589415836904 29.245646895808715 22.092179574964252 46-47 22.262381090362076 27.367365276844446 28.772741564582656 21.597512068210822 48-49 22.100056563962745 27.33380290156175 28.64533162357068 21.920808910904825 50-51 21.57446018188782 27.64036735143683 28.98583784225343 21.79933462442192 52-53 22.0013671835864 28.18906746365095 27.926381710875354 21.8831836418873 54-55 21.683639454969637 27.519154600225516 28.387113680558073 22.410092264246774 56-57 22.157916032446387 27.703512456407392 28.00215795456735 22.136413556578873 58-59 22.626389670802492 27.38869628446216 28.06889368505381 21.916020359681543 60-61 22.202711919266648 27.501435336639425 28.863059251467128 21.4327934926268 62-63 21.85759310874482 27.58814160922092 28.812469244413514 21.74179603762075 64-65 21.650008496379694 27.980023296649264 28.464220608402435 21.9057475985686 66-67 21.883969104368145 27.745482953330114 27.92739426308769 22.44315367921405 68-69 21.70063343643894 27.727030219297298 28.35225573068774 22.22008061357602 70-71 22.28869931052404 27.548271606413827 28.085417446598676 22.07761163646346 72-73 22.014441991206876 27.280913683285746 28.601847249355544 22.102797076151834 74-75 21.567609624809098 28.40084796481123 27.92264421355734 22.10889819682233 76-77 21.901156422682007 27.677835732944445 27.939905793521163 22.481102050852382 78-79 21.930544994444418 27.761652946964315 28.03694626750741 22.270855791083857 80-81 22.148605705661357 27.94904907572111 27.767582777128457 22.134762441489073 82-83 21.99424182041726 27.767799673934405 28.145897007982057 22.09206149766628 84-85 21.886322315419044 27.97425496707882 27.803040170544275 22.336382546957864 86-87 21.81891204151593 28.104078129131377 27.916410031474587 22.160599797878106 88-89 21.944317230340797 27.877660124006766 27.46398298877586 22.71403965687658 90-91 21.912556236178265 27.54814310269128 27.890600275307197 22.648700385823258 92-93 22.241110802726123 27.827312116446173 27.890916520183794 22.040660560643907 94-95 21.972683232863048 27.704633938903946 27.855426069658794 22.46725675857421 96-97 21.375876778365306 27.814621855669785 28.32004236051993 22.489459005444974 98-99 21.632057097020763 28.03920563415244 27.540847875031655 22.787889393795137 100-101 21.624610543850448 27.76610266218342 27.786423789986735 22.822863003979393 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 469.0 1 343.5 2 278.5 3 334.0 4 287.0 5 304.5 6 495.0 7 960.0 8 1671.0 9 2042.0 10 1836.0 11 1636.5 12 1672.5 13 1819.0 14 1978.0 15 2070.5 16 2121.0 17 2186.5 18 2352.5 19 2569.5 20 2816.0 21 3194.0 22 3760.5 23 4525.0 24 5406.5 25 6635.5 26 8301.0 27 10155.0 28 11722.5 29 13619.0 30 16114.5 31 18627.0 32 21299.5 33 24108.5 34 27119.5 35 29883.5 36 33424.5 37 36851.0 38 38572.0 39 39563.0 40 39923.0 41 40615.0 42 41279.0 43 42266.5 44 44160.0 45 45525.0 46 46341.5 47 51299.0 48 63451.0 49 62795.5 50 50332.0 51 44451.5 52 41766.0 53 40211.0 54 38585.0 55 35362.5 56 31596.5 57 28397.5 58 25838.0 59 22747.0 60 19116.0 61 15473.5 62 11969.0 63 8962.5 64 6400.5 65 4644.0 66 3371.0 67 2418.5 68 1696.0 69 1183.5 70 814.0 71 552.5 72 380.0 73 266.0 74 184.5 75 128.0 76 91.0 77 48.5 78 30.5 79 20.5 80 12.5 81 10.5 82 10.0 83 5.5 84 3.5 85 4.0 86 5.0 87 4.0 88 2.5 89 1.0 90 0.5 91 0.5 92 0.5 93 1.0 94 1.0 95 2.5 96 2.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03722110182167625 2 0.02735712452731898 3 0.024274631622832332 4 0.0441567108567712 5 0.09386190894161836 6 0.11274217798159906 7 0.05533074763553529 8 0.08530799113166791 9 0.07112852377102935 10-11 0.07336333112678217 12-13 0.07193767815845709 14-15 0.06488647563944389 16-17 0.05082260176272357 18-19 0.06180398273495724 20-21 0.058220584733491516 22-23 0.033483579174986185 24-25 0.010249288907418096 26-27 0.002774243614037981 28-29 0.007667701099910531 30-31 0.005702611873300294 32-33 0.0065117662607280385 34-35 0.007089733680319284 36-37 0.006049392325055042 38-39 0.004007240775832639 40-41 0.008977760584317354 42-43 0.0048549263245664664 44-45 0.0032751487110170606 46-47 0.002080682710528486 48-49 3.853116130608307E-4 50-51 0.002928368259262313 52-53 0.0071667960029314515 54-55 0.014025342715414236 56-57 0.009709852649132933 58-59 0.006665890905952371 60-61 0.003544866840159642 62-63 0.00944013451999035 64-65 0.0030439617431805626 66-67 0.024313162784138417 68-69 0.04616033124468752 70-71 0.06138013996059032 72-73 0.003544866840159642 74-75 0.014757434780229815 76-77 0.02219394891230385 78-79 0.023696664203241086 80-81 0.07640729286996273 82-83 0.02820481007605281 84-85 0.01217584697272225 86-87 0.03190380156143678 88-89 0.048163951632603834 90-91 0.042731057888446125 92-93 0.04400258621154686 94-95 0.013062063682762162 96-97 0.016683992845533967 98-99 0.03779906924126749 100-101 0.07482751525641332 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1297651.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.80244485802151 #Duplication Level Percentage of deduplicated Percentage of total 1 81.18596694198565 50.98677212119212 2 11.547524935494092 14.504255960159924 3 3.261814037002119 6.1454968858783845 4 1.3755922196829493 3.4556221809504732 5 0.6895519246578252 2.1652773362532836 6 0.426354956649568 1.6065680192957188 7 0.27834279251433985 1.2236425514956688 8 0.1917182830783165 0.9632301521040425 9 0.14538728763096245 0.8217609403050748 >10 0.8208871949587905 9.409095675499637 >50 0.05148804666695303 2.1982790826605756 >100 0.021676547921314043 2.3872097766237883 >500 0.0017242548199619303 0.7696914993028782 >1k 0.0017242548199619303 2.3676270519205205 >5k 2.463221171374186E-4 0.9954707663579582 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6775 0.5220972356974256 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6095 0.46969485632115265 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4997 0.3850804260929942 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3860 0.2974605652829613 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3209 0.24729299326244114 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3033 0.2337300244826999 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2300 0.1772433420079821 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2092 0.16121437890465157 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1739 0.13401137902255691 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1721 0.13262425721553794 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1556 0.1199089739845305 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 7.706232261216614E-5 0.0 0.0 0.0 3 0.0 7.706232261216614E-5 0.0 7.706232261216614E-5 0.0 4 0.0 7.706232261216614E-5 0.0 7.706232261216614E-5 0.0 5 0.0 1.5412464522433227E-4 0.0 7.706232261216614E-5 0.0 6 0.0 1.5412464522433227E-4 0.0 7.706232261216614E-5 0.0 7 0.0 1.5412464522433227E-4 0.0 7.706232261216614E-5 0.0 8 0.0 1.5412464522433227E-4 0.0 7.706232261216614E-5 0.0 9 0.0 1.5412464522433227E-4 0.0 1.5412464522433227E-4 0.0 10-11 7.706232261216614E-5 1.5412464522433227E-4 0.0 1.9265580653041533E-4 0.0 12-13 7.706232261216614E-5 1.5412464522433227E-4 0.0 3.0824929044866455E-4 0.0 14-15 7.706232261216614E-5 1.5412464522433227E-4 0.0 3.0824929044866455E-4 0.0 16-17 7.706232261216614E-5 1.5412464522433227E-4 0.0 3.8531161306083067E-4 0.0 18-19 1.1559348391824921E-4 1.5412464522433227E-4 0.0 4.6237393567299685E-4 0.0 20-21 1.5412464522433227E-4 1.5412464522433227E-4 0.0 4.6237393567299685E-4 0.0 22-23 1.5412464522433227E-4 1.5412464522433227E-4 0.0 8.091543874277445E-4 0.0 24-25 1.5412464522433227E-4 1.9265580653041533E-4 0.0 9.247478713459937E-4 0.0 26-27 1.5412464522433227E-4 2.3118696783649842E-4 0.0 0.0011559348391824921 0.0 28-29 1.9265580653041533E-4 2.3118696783649842E-4 0.0 0.001733902258773738 0.0 30-31 2.3118696783649842E-4 3.0824929044866455E-4 0.0 0.0039687096145265555 0.0 32-33 2.3118696783649842E-4 3.8531161306083067E-4 0.0 0.006897077873788869 0.0 34-35 2.6971812914258146E-4 3.8531161306083067E-4 0.0 0.010364882391336346 0.0 36-37 3.0824929044866455E-4 3.8531161306083067E-4 0.0 0.013216188327986492 0.0 38-39 3.8531161306083067E-4 3.8531161306083067E-4 0.0 0.015373933361127145 0.0 40-41 4.2384277436691376E-4 3.8531161306083067E-4 0.0 0.01818670813647121 0.0 42-43 4.6237393567299685E-4 4.2384277436691376E-4 0.0 0.020845358266590938 0.0 44-45 4.6237393567299685E-4 4.6237393567299685E-4 0.0 0.024390225106750582 0.0 46-47 5.394362582851629E-4 4.6237393567299685E-4 0.0 0.02974605652829613 0.0 48-49 5.394362582851629E-4 4.6237393567299685E-4 0.0 0.036797259047309336 0.0 50-51 5.394362582851629E-4 4.6237393567299685E-4 0.0 0.041921903501018376 0.0 52-53 5.394362582851629E-4 4.6237393567299685E-4 0.0 0.051130851053172235 0.0 54-55 5.394362582851629E-4 5.394362582851629E-4 0.0 0.06893224757658262 0.0 56-57 5.394362582851629E-4 5.394362582851629E-4 0.0 0.10908171765752117 0.0 58-59 5.394362582851629E-4 6.935609035094953E-4 0.0 0.15273752341731328 0.0 60-61 5.394362582851629E-4 6.935609035094953E-4 0.0 0.1916154651751511 0.0 62-63 6.164985808973291E-4 6.935609035094953E-4 0.0 0.24120506977608 0.0 64-65 6.164985808973291E-4 7.706232261216613E-4 0.0 0.3003504023809175 0.0 66-67 6.935609035094953E-4 7.706232261216613E-4 0.0 0.38808585667486867 0.0 68-69 6.935609035094953E-4 7.706232261216613E-4 0.0 0.49978769330120343 0.0 70-71 6.935609035094953E-4 7.706232261216613E-4 0.0 0.5951908486950652 0.0 72-73 6.935609035094953E-4 7.706232261216613E-4 0.0 0.6983002363501434 0.0 74-75 6.935609035094953E-4 7.706232261216613E-4 0.0 0.804761835038851 0.0 76-77 6.935609035094953E-4 7.706232261216613E-4 0.0 0.9310669818001913 0.0 78-79 6.935609035094953E-4 7.706232261216613E-4 0.0 1.0294755677759273 0.0 80-81 9.247478713459937E-4 7.706232261216613E-4 0.0 1.131351958269211 0.0 82-83 9.247478713459937E-4 7.706232261216613E-4 0.0 1.2440941362508102 0.0 84-85 0.0010018101939581598 7.706232261216613E-4 0.0 1.4292363663265393 0.0 86-87 0.0010788725165703258 7.706232261216613E-4 0.0 1.6734853978457997 0.0 88-89 0.0010788725165703258 7.706232261216613E-4 0.0 1.9477502040224992 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7280 0.0 24.02743 1 TACATGG 7430 0.0 22.64847 2 GAGTACT 5630 0.0 22.4137 12-13 AGTACTT 5895 0.0 21.768944 12-13 ACATGGG 7475 0.0 21.431862 3 GTACTTT 5910 0.0 21.350561 14-15 CCTATAC 315 1.4551915E-11 21.128012 3 CATGGGA 5005 0.0 19.756063 4 TACTTTT 6720 0.0 19.378204 14-15 AGAGTAC 11065 0.0 19.370865 10-11 GTATCAA 16195 0.0 19.194983 1 ACTTTTT 7030 0.0 18.453594 16-17 GTGTAGC 740 0.0 17.985281 1 TTGCGTT 240 3.0672036E-6 17.826761 4 TTTGCGT 240 3.0672036E-6 17.826761 3 CGTTTAG 120 3.0888768E-6 17.815075 90-91 ATGGGAG 2160 0.0 17.386593 5 CAACGCA 18270 0.0 17.042917 5 TCAACGC 18450 0.0 16.90241 4 TATCAAC 18400 0.0 16.896017 2 >>END_MODULE