FastQCFastQC Report
Thu 26 May 2016
SRR936884_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936884_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297651
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76840.5921468869518846No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT70700.5448306208680146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59830.46106387618859No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42110.3245094405198316No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31960.246291183068483No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26500.20421515492224027No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT21230.1636033109056287No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC20600.15874838458106225No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC20150.15528058006351475No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC18020.13886630534712338No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15920.1226832175985685No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15820.12191259437244684No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG15420.11883010146796018No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA13600.10480475875254594No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC13310.10256995139679313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG1652.4314159E-623.029075
GAGTACT66300.022.10228212-13
GTACATG68550.021.8318541
TACATGG69250.021.3995762
ACATGGG69800.020.7546183
GTACTTT70950.020.7533414-15
AGTACTT68950.019.66832212-13
AGAGTAC118300.018.89203510-11
GTATCAA178900.018.0852411
ACTTTTT84900.017.48286816-17
CATGGGA50050.017.4616034
CATGGGG23800.017.3625164
TACTTTT80600.016.64800314-15
TTAGACT5200.016.4414274
CTTACAC5250.016.2848423
AGACCCG2053.333812E-416.2186435
CAACGCA198200.016.1520125
ATCAACG198600.016.1433963
TCAACGC198600.016.1433964
TATCAAC200250.015.9866592
Produced by FastQC (version 0.11.5)