##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936884_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1297651 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.027932009454005 31.0 31.0 34.0 30.0 34.0 2 32.14690159372589 33.0 31.0 34.0 30.0 34.0 3 32.276409450615 34.0 31.0 34.0 30.0 34.0 4 35.9642561829028 37.0 35.0 37.0 35.0 37.0 5 35.80382938093524 37.0 35.0 37.0 35.0 37.0 6 35.89867383449017 37.0 35.0 37.0 35.0 37.0 7 35.85770750378954 37.0 35.0 37.0 35.0 37.0 8 35.80546079030494 37.0 35.0 37.0 35.0 37.0 9 37.47234580021901 39.0 37.0 39.0 35.0 39.0 10-11 37.38133596783727 39.0 37.0 39.0 34.0 39.0 12-13 37.27914978680708 39.0 37.0 39.0 34.0 39.0 14-15 38.40537209157162 40.0 38.0 41.0 34.0 41.0 16-17 38.4206416054856 40.0 38.0 41.0 33.5 41.0 18-19 38.58520896604711 40.0 38.0 41.0 34.0 41.0 20-21 38.60021415619454 40.0 38.0 41.0 34.0 41.0 22-23 38.50549878202999 40.0 38.0 41.0 34.0 41.0 24-25 38.408748962548486 40.0 38.0 41.0 34.0 41.0 26-27 38.193016072888625 40.0 38.0 41.0 33.5 41.0 28-29 38.046015839389796 40.0 37.0 41.0 33.0 41.0 30-31 38.015695283246416 40.0 37.0 41.0 33.0 41.0 32-33 37.858224977285886 40.0 37.0 41.0 33.0 41.0 34-35 37.718227011731194 40.0 37.0 41.0 33.0 41.0 36-37 37.54923781509821 40.0 36.5 41.0 33.0 41.0 38-39 37.3834374573749 40.0 36.0 41.0 32.5 41.0 40-41 37.07990630762817 39.5 36.0 41.0 31.5 41.0 42-43 36.851497051210224 39.0 36.0 41.0 31.0 41.0 44-45 36.585780768480895 39.0 35.5 41.0 30.5 41.0 46-47 36.17353317648582 39.0 35.0 40.5 30.0 41.0 48-49 35.75376545773864 39.0 35.0 40.0 28.5 41.0 50-51 35.453255536349914 39.0 35.0 40.0 27.5 41.0 52-53 35.20466789606759 38.0 35.0 40.0 27.0 41.0 54-55 34.830806588212084 38.0 34.0 40.0 26.0 41.0 56-57 34.62110844903599 38.0 34.0 40.0 26.0 41.0 58-59 34.30468554333946 38.0 34.0 40.0 25.0 41.0 60-61 33.90973112185017 37.0 33.0 40.0 24.0 41.0 62-63 33.5488983555671 37.0 33.0 39.0 23.0 40.5 64-65 33.21581804352634 36.0 32.0 39.0 22.5 40.0 66-67 32.86552393517209 36.0 32.0 39.0 22.0 40.0 68-69 32.40152745229649 35.0 31.5 38.5 20.5 40.0 70-71 31.94577509669395 35.0 31.0 38.0 19.5 40.0 72-73 31.52313988892237 35.0 31.0 37.0 18.5 39.0 74-75 31.04329438346674 34.5 30.5 36.5 17.5 39.0 76-77 29.60792732406479 33.0 28.5 35.0 13.0 37.5 78-79 30.291136831089407 34.0 30.0 36.0 13.5 37.5 80-81 30.142397300969215 34.0 30.0 35.0 9.5 37.0 82-83 29.777178532594665 34.0 29.5 35.0 7.0 37.0 84-85 29.32187814751424 34.0 29.0 35.0 4.5 36.0 86-87 28.967086296700728 34.0 29.0 35.0 2.0 36.0 88-89 28.5765853068352 33.5 29.0 35.0 2.0 35.5 90-91 28.086396881750176 33.0 27.5 35.0 2.0 35.0 92-93 27.594914194956885 33.0 27.0 35.0 2.0 35.0 94-95 27.00359148954534 32.5 25.5 34.0 2.0 35.0 96-97 26.18713621767332 32.0 25.0 34.0 2.0 35.0 98-99 25.301994527033848 32.0 22.0 34.0 2.0 35.0 100-101 22.06714170451069 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18501375435900513 1101 2 0.3867257524455425 1101 3 0.3602804092784311 1101 4 0.2262888679727837 1101 5 0.19512570940981533 1101 6 0.24668769138538238 1101 7 0.1875116028928545 1101 8 0.2514808707493188 1101 9 0.3385257013943388 1101 10-11 0.38706838881490313 1101 12-13 0.3469357962431303 1101 14-15 0.532795865954725 1101 16-17 0.569042547898384 1101 18-19 0.3710951802507694 1101 20-21 0.2865610852792173 1101 22-23 0.36144545686408946 1101 24-25 0.355416803837592 1101 26-27 0.31844527824991786 1101 28-29 0.26027939526155563 1101 30-31 0.29795378538608475 1101 32-33 0.30953598965230356 1101 34-35 0.2576535283710726 1101 36-37 0.28833082576056057 1101 38-39 0.4030200169187097 1101 40-41 0.5720862176053387 1101 42-43 0.5463356223192122 1101 44-45 0.3719856812562554 1101 46-47 0.09776036603407334 1101 48-49 0.1447915195556817 1101 50-51 0.18345558860369948 1101 52-53 0.3949688496398167 1101 54-55 0.16725669073122873 1101 56-57 0.29120787564408346 1101 58-59 0.22988290911757048 1101 60-61 0.21487371378997722 1101 62-63 0.1768228856948042 1101 64-65 0.22308704109968147 1101 66-67 0.2661023014021495 1101 68-69 -0.004806543393968354 1101 70-71 0.08220185172157812 1101 72-73 -0.0012380120575414821 1101 74-75 -0.24787775960334457 1101 76-77 -0.2263700792909269 1101 78-79 -0.04876216213333251 1101 80-81 -0.17295152757341015 1101 82-83 0.15167125513590918 1101 84-85 -0.09744297326992424 1101 86-87 -0.20289245661372135 1101 88-89 0.034975932380458374 1101 90-91 0.14410785994842712 1101 92-93 -0.10082113124650505 1101 94-95 -0.016935429994543227 1101 96-97 -0.2271147803441842 1101 98-99 -0.040395310348131375 1101 100-101 0.7708452631266276 1102 1 -0.056297897282611586 1102 2 -0.00576343005894131 1102 3 0.15386764167002553 1102 4 0.12935036738812755 1102 5 0.07632994049199482 1102 6 0.19360921806674014 1102 7 0.11163556088379067 1102 8 0.27369108850025015 1102 9 0.2910627610832037 1102 10-11 0.35872634849783935 1102 12-13 0.300614216215223 1102 14-15 0.25986594123263984 1102 16-17 0.20842422632104984 1102 18-19 0.37275417722779736 1102 20-21 0.3059541065801241 1102 22-23 0.17723347706034076 1102 24-25 0.19405392064498983 1102 26-27 0.4315419917789356 1102 28-29 0.29249242852501567 1102 30-31 0.277748778354038 1102 32-33 0.130215120911771 1102 34-35 0.3335794143818802 1102 36-37 0.3176077778941533 1102 38-39 0.4443814452053161 1102 40-41 0.38223007817146026 1102 42-43 0.5399000207694655 1102 44-45 0.4145157493135301 1102 46-47 0.10361030056330378 1102 48-49 0.11682196211653206 1102 50-51 0.04503374399843807 1102 52-53 0.1483393818659806 1102 54-55 0.27513527613151467 1102 56-57 0.33137439502397825 1102 58-59 0.08983305164447586 1102 60-61 0.007583624394094102 1102 62-63 0.0037500299298685036 1102 64-65 0.21473076156374304 1102 66-67 0.02470173883849469 1102 68-69 0.11913595548790568 1102 70-71 -0.19662111585557085 1102 72-73 -0.20152771347850162 1102 74-75 -0.019336949490135424 1102 76-77 0.23660727806093007 1102 78-79 -0.016036543932997915 1102 80-81 0.2587426565745794 1102 82-83 0.35521563515735366 1102 84-85 0.31256334479407855 1102 86-87 0.13091922197060413 1102 88-89 -0.030325435231180364 1102 90-91 -0.06198226878380808 1102 92-93 0.12749470729125534 1102 94-95 0.07390223213317526 1102 96-97 0.11943406138898638 1102 98-99 0.21224069641987242 1102 100-101 0.4499297096426673 1103 1 0.07710244413955536 1103 2 0.19592325926284104 1103 3 0.10722171560228588 1103 4 0.1422101768936983 1103 5 0.022576689801972805 1103 6 0.09428280890258378 1103 7 0.11143836565094034 1103 8 0.08094327822887237 1103 9 0.10927300693906972 1103 10-11 0.137179737990337 1103 12-13 0.23333139421743 1103 14-15 0.25507901100082364 1103 16-17 0.1332481705447961 1103 18-19 0.09048292210248121 1103 20-21 0.1327190517770731 1103 22-23 0.2698463138956484 1103 24-25 0.24870890875903484 1103 26-27 0.1869876753412072 1103 28-29 0.05514467249827959 1103 30-31 0.16752462034258286 1103 32-33 0.2676566882628748 1103 34-35 0.2988287004266539 1103 36-37 0.3287323969850746 1103 38-39 0.21496489420736964 1103 40-41 0.3083766344343246 1103 42-43 0.09737477307885456 1103 44-45 0.12442465022132154 1103 46-47 0.19647936356685847 1103 48-49 0.10925100533675192 1103 50-51 -0.05768614623593038 1103 52-53 -0.07704647304554868 1103 54-55 0.13327149400353022 1103 56-57 -0.13674985711789134 1103 58-59 -0.06625622865674075 1103 60-61 -0.2987728905471201 1103 62-63 0.05438261819707435 1103 64-65 -0.12253690384252991 1103 66-67 0.022455372431885223 1103 68-69 -0.09670954300440826 1103 70-71 -0.23485210231036646 1103 72-73 -0.02530728877485089 1103 74-75 -0.20798099294966477 1103 76-77 -0.1056812077158078 1103 78-79 0.10304831702521966 1103 80-81 -0.06262494660989759 1103 82-83 0.1569170024414568 1103 84-85 -0.20158302001710027 1103 86-87 -0.3364851718455064 1103 88-89 -0.05270326512636103 1103 90-91 -0.29735169075927104 1103 92-93 0.003668545418861413 1103 94-95 0.14326421940164025 1103 96-97 0.07665258753011983 1103 98-99 0.001744996105575325 1103 100-101 0.7756949579714529 1104 1 0.14670569885797846 1104 2 0.26655187264267255 1104 3 0.06292450841988284 1104 4 0.14237352460408204 1104 5 -0.09036571024697082 1104 6 0.037331966162994945 1104 7 0.09202751862882508 1104 8 0.13057345167110412 1104 9 -0.11863069744439514 1104 10-11 0.10504642912856355 1104 12-13 0.18638819884255042 1104 14-15 0.2365925774258315 1104 16-17 0.22254920140955647 1104 18-19 0.15260766591575958 1104 20-21 0.3243635410860577 1104 22-23 0.2607529854566266 1104 24-25 0.20253779132610106 1104 26-27 0.1875743811623849 1104 28-29 0.15899221463525492 1104 30-31 0.08611719933985995 1104 32-33 0.1299796517696521 1104 34-35 0.0658459222919916 1104 36-37 0.03287512740017462 1104 38-39 0.05981209986030933 1104 40-41 0.08846106134080856 1104 42-43 -0.13844976578504742 1104 44-45 0.01235190848812806 1104 46-47 -0.2401778203675633 1104 48-49 -0.23685618012714116 1104 50-51 -0.0080388426967275 1104 52-53 -0.04082580068924102 1104 54-55 0.034674632482015966 1104 56-57 -0.09934690217616549 1104 58-59 -0.39020379832422947 1104 60-61 -0.20594214202305494 1104 62-63 -0.19006233607168355 1104 64-65 0.05387709389384554 1104 66-67 -0.13811787402737963 1104 68-69 -0.03216058457549309 1104 70-71 -0.04543850223695145 1104 72-73 -0.06252793054170169 1104 74-75 -0.3745075598946954 1104 76-77 -0.1888937126865926 1104 78-79 0.07809557773917675 1104 80-81 -0.06594390289756191 1104 82-83 0.08581608781663519 1104 84-85 -0.08884648702636966 1104 86-87 -0.31384866638528663 1104 88-89 -0.2553590419456704 1104 90-91 -0.2748644618583498 1104 92-93 -0.34958005511339607 1104 94-95 -0.131625199704537 1104 96-97 0.06261967327237272 1104 98-99 0.172209038196673 1104 100-101 1.018412831392272 1105 1 0.027068637513458782 1105 2 0.10433885538634513 1105 3 0.04748766106092717 1105 4 0.05149122264533901 1105 5 -0.01406969288903781 1105 6 0.11876782129834851 1105 7 0.11871355158738339 1105 8 0.0661404835477768 1105 9 0.15917158706077572 1105 10-11 0.017382577746928973 1105 12-13 0.18599310257893364 1105 14-15 0.31742293707699787 1105 16-17 -0.0485232966627791 1105 18-19 0.04771528631253119 1105 20-21 0.24745874075611596 1105 22-23 0.12597883586995096 1105 24-25 0.009547313086279985 1105 26-27 0.04999421128892578 1105 28-29 -0.06877689612917237 1105 30-31 0.03009392392608845 1105 32-33 -0.08352654365057788 1105 34-35 -0.16461056033512733 1105 36-37 -0.12717023993176468 1105 38-39 -0.18494495478969952 1105 40-41 0.026334714217959743 1105 42-43 0.054264563477772754 1105 44-45 -0.08070043429524532 1105 46-47 -0.10465203151991176 1105 48-49 -0.0804898105298264 1105 50-51 -0.011880421647241235 1105 52-53 0.22443651095264983 1105 54-55 0.5535194092615896 1105 56-57 0.054658752302891855 1105 58-59 0.004841359803926082 1105 60-61 0.09682323295975692 1105 62-63 0.3143330351454594 1105 64-65 0.3249050614493285 1105 66-67 0.19660457234173379 1105 68-69 0.016596745028039095 1105 70-71 0.032454636477105225 1105 72-73 -0.030617182847421986 1105 74-75 0.06086282043775881 1105 76-77 0.008292197738331453 1105 78-79 0.20089370805519025 1105 80-81 -0.011464252447176193 1105 82-83 0.10212322286065856 1105 84-85 -0.182169001536149 1105 86-87 -0.2086690798914823 1105 88-89 0.1727328403503101 1105 90-91 0.1004982078208485 1105 92-93 0.17784028307944055 1105 94-95 0.12063181011608393 1105 96-97 0.6318024635724875 1105 98-99 0.6618486697589461 1105 100-101 0.7989494091772222 1106 1 0.08126320956932176 1106 2 0.151113515140473 1106 3 0.13976947627958936 1106 4 0.13199773236532764 1106 5 0.10042265965058306 1106 6 0.12722461483843972 1106 7 0.12148840813984663 1106 8 0.3116070493552954 1106 9 0.25557017749268596 1106 10-11 0.3143927805107154 1106 12-13 0.352713888788287 1106 14-15 0.4135619997019191 1106 16-17 0.27661738339847375 1106 18-19 0.23343479948264445 1106 20-21 0.17686502477301502 1106 22-23 0.294275546262746 1106 24-25 0.25868939533568636 1106 26-27 0.2037484470656281 1106 28-29 0.3100353029077425 1106 30-31 0.27719729840256946 1106 32-33 0.22684688119397833 1106 34-35 0.2721529065135826 1106 36-37 0.26296555092080354 1106 38-39 0.3154889240440326 1106 40-41 0.2695691563869218 1106 42-43 0.44712078384271337 1106 44-45 0.15929513774217696 1106 46-47 0.09902932737260528 1106 48-49 0.0998005285168091 1106 50-51 0.20059321045057033 1106 52-53 0.2286618327486778 1106 54-55 0.33478882003506527 1106 56-57 0.1455171620493232 1106 58-59 0.2378853380823145 1106 60-61 0.3249072781495741 1106 62-63 0.1668538988441668 1106 64-65 0.1286417698741431 1106 66-67 0.08973262040972685 1106 68-69 -0.05873673590404849 1106 70-71 0.1575442719696838 1106 72-73 0.19170589852253883 1106 74-75 0.3075114062572091 1106 76-77 0.315859093798057 1106 78-79 0.3015914196595304 1106 80-81 0.42303111356698153 1106 82-83 0.4908300732877713 1106 84-85 0.5440236271361414 1106 86-87 0.42921306250441305 1106 88-89 0.5791284788071671 1106 90-91 0.3378775919650536 1106 92-93 0.3721134514711686 1106 94-95 0.5853461757796943 1106 96-97 0.676769038604931 1106 98-99 0.646779144596433 1106 100-101 0.839698042351273 1107 1 0.30464792016128683 1107 2 0.28228281513337805 1107 3 0.25497508200908925 1107 4 0.09513245423837446 1107 5 0.0625955889278842 1107 6 0.11681056699377024 1107 7 0.15682461653683788 1107 8 0.179993597151622 1107 9 0.28909819002435455 1107 10-11 0.324999251337843 1107 12-13 0.28094301717986525 1107 14-15 0.25720043788482627 1107 16-17 0.29044637512782856 1107 18-19 0.3108136568059976 1107 20-21 0.19317263079904734 1107 22-23 0.1118118229908518 1107 24-25 0.16807106325938292 1107 26-27 0.212510944889992 1107 28-29 0.2400584452362935 1107 30-31 0.17784354309240769 1107 32-33 0.171507385168141 1107 34-35 0.32074696170705863 1107 36-37 0.43190745013887266 1107 38-39 0.3976013222725783 1107 40-41 0.5514004945807898 1107 42-43 0.45099631808239593 1107 44-45 0.3438529516623845 1107 46-47 0.34585805480239173 1107 48-49 0.4434773223695814 1107 50-51 0.27502351594219476 1107 52-53 0.20332678469127785 1107 54-55 0.37309195230734105 1107 56-57 0.18550858842900197 1107 58-59 0.34958123886038805 1107 60-61 0.1797388032870444 1107 62-63 0.06824985183751409 1107 64-65 0.3112689076386843 1107 66-67 0.30537528738582154 1107 68-69 0.17729470929891988 1107 70-71 -0.1953623787100831 1107 72-73 0.18628260019278642 1107 74-75 0.05921484924263254 1107 76-77 -0.013500721063312682 1107 78-79 0.20570836899781142 1107 80-81 0.28615842215929277 1107 82-83 0.4051144983072703 1107 84-85 0.4186295951494472 1107 86-87 0.8311453502428456 1107 88-89 0.817421941582829 1107 90-91 0.484018495869595 1107 92-93 0.48134395293126175 1107 94-95 0.669798592186897 1107 96-97 0.6333027174616106 1107 98-99 0.8556122297460185 1107 100-101 0.9179864769886947 1108 1 0.004723672271524038 1108 2 0.1417422095974672 1108 3 0.028401121496379744 1108 4 0.016544449524907634 1108 5 -0.05711979957222013 1108 6 0.0762471155833424 1108 7 0.01638200777844645 1108 8 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Count 3 6.0 4 13.0 5 28.0 6 72.0 7 185.0 8 427.0 9 800.0 10 1391.0 11 2218.0 12 3345.0 13 4767.0 14 6143.0 15 8584.0 16 11072.0 17 15347.0 18 13540.0 19 8286.0 20 7056.0 21 7240.0 22 7866.0 23 8648.0 24 10067.0 25 12034.0 26 14516.0 27 18153.0 28 22754.0 29 28387.0 30 35086.0 31 44093.0 32 55681.0 33 71374.0 34 96364.0 35 134240.0 36 198487.0 37 277092.0 38 164356.0 39 7933.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.11674157363994 20.03334394440518 15.618238813862018 21.231675668092866 2 15.33729140368156 21.878280088964104 39.539319452343626 23.245109055010705 3 19.890309151872582 25.982489845081137 30.37343746026462 23.753763542781666 4 12.714897919394353 17.67270244464806 38.28086288223875 31.331536753718837 5 13.025512189671051 38.557353298166255 33.991515202891456 14.425619309271239 6 29.921373312238806 37.77117268048188 17.497154473737545 14.810299533541762 7 26.944014179478287 34.11258814009941 21.101915000192655 17.841482680229646 8 26.180459923353816 33.977933974543234 19.86882451444957 19.972781587653383 9 26.20727761162285 17.23298483182304 21.152220435232586 35.407517121321526 10-11 24.437310185866615 27.353271411188366 27.246501563209215 20.962916839735797 12-13 26.377762518755883 24.97751606243252 27.738204506343667 20.906516912467932 14-15 22.962484876041337 25.920076139980424 27.471967694453646 23.64547128952459 16-17 21.10783253740798 29.2093559824637 27.750835933544536 21.931975546583786 18-19 20.93910639129163 27.7357466216776 30.680226579077463 20.64492040795331 20-21 22.414336531970445 26.109402020289203 30.611396416252322 20.864865031488026 22-23 21.771821990142072 26.315815837415112 30.598025948725223 21.314336223717593 24-25 21.81902950928318 26.50421822651462 30.703949662561143 20.972802601641053 26-27 21.645207962871275 26.19690632471453 31.19953608356661 20.95834962884759 28-29 21.10129850113667 27.056217007667705 30.900088621739297 20.942395869456327 30-31 21.423826591279166 26.39897013912061 30.732300133086632 21.444903136513595 32-33 21.4711319796524 26.562385610262268 30.72052723121157 21.245955178873764 34-35 22.01832387907072 26.410336831705905 30.57987085895977 20.99146843026361 36-37 21.794110054059885 26.197929309859862 30.90002196303275 21.107938673047506 38-39 21.50147724632191 26.738871327548686 30.724064568014793 21.035586858114605 40-41 21.720189637155567 26.66699290093678 30.444053648326648 21.168763813581005 42-43 21.361945546221595 26.893440532161577 29.897098680615976 21.847515241000853 44-45 21.73380639652539 26.592272753193868 29.853033425243638 21.820887425037107 46-47 22.29990544438442 27.109565067287072 29.300142026079474 21.290387462249036 48-49 22.14001794015018 27.192736396927366 29.04720694672207 21.620038716200387 50-51 21.619655465254457 27.405416319239478 29.363672653460064 21.611255562046 52-53 22.13522712079137 27.897505214247488 28.34381320336471 21.623454461596438 54-55 21.785865382911123 27.3249510076284 28.705252799096215 22.183930810364267 56-57 22.186769231599136 27.47313508000427 28.38566962804671 21.954426060349885 58-59 22.668409304669712 27.167582617648296 28.426165925390485 21.73784215229151 60-61 22.16631653902292 27.37422762561054 29.204036306412807 21.25541952895373 62-63 21.908865291540362 27.297488807656606 29.139779933040522 21.653865967762506 64-65 21.70966747582168 27.733210033522138 28.79620853080569 21.7609139598505 66-67 22.05817359913166 27.38838385220057 28.382642321642077 22.170800227025687 68-69 21.78092843288131 27.40737287201701 28.68791946050126 22.12377923460042 70-71 22.268112976795692 27.32584439373728 28.468450973184538 21.93759165628249 72-73 22.069775470697756 27.102342083523425 28.87223653872237 21.95564590705646 74-75 21.673044385610076 28.14163506706334 28.18190120116383 22.003419346162755 76-77 21.935331486145902 27.406150432879166 28.24697905480567 22.411539026169258 78-79 21.954811372408344 27.545612278199933 28.35045210590379 22.149124243487936 80-81 22.207002155469706 27.682681259435476 28.06726730059208 22.043049284502736 82-83 22.07744769390822 27.472739182368127 28.410626902477553 22.039186221246098 84-85 21.807390750156824 27.805747744720726 28.09092324267005 22.295938262452395 86-87 21.867439449126632 27.79373613116341 28.01540632921781 22.323418090492147 88-89 21.931189408301353 27.585001772475763 27.759629167244647 22.72417965197824 90-91 21.909957800184333 27.293632420802133 28.061991190133444 22.734418588880086 92-93 22.211249403730278 27.603338648064852 28.080007644582405 22.10540430362247 94-95 21.863621266426797 27.42744389670258 28.103318997172583 22.60561583969804 96-97 21.466543931932314 27.47136264453537 28.43868856729032 22.623404856241997 98-99 21.655658279948245 27.720597358141312 27.678290012615143 22.945454349295304 100-101 21.40547972019565 27.541362231344902 27.905836912318776 23.147321136140675 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 658.0 1 483.5 2 330.5 3 363.5 4 334.0 5 323.5 6 517.0 7 1149.5 8 1913.5 9 2299.0 10 2102.5 11 1749.0 12 1727.0 13 1904.5 14 2129.5 15 2258.0 16 2388.5 17 2529.0 18 2653.0 19 2843.5 20 3175.0 21 3585.5 22 4051.5 23 4783.5 24 5670.5 25 6782.0 26 8384.0 27 10240.5 28 11829.5 29 13735.0 30 16125.5 31 18387.5 32 21003.5 33 23853.5 34 26831.0 35 29643.0 36 33288.0 37 36850.0 38 38375.5 39 39287.0 40 39837.0 41 40691.0 42 41241.5 43 42070.5 44 44176.5 45 45517.5 46 45938.0 47 51199.0 48 63985.0 49 63086.0 50 49889.0 51 43937.0 52 41658.0 53 40037.5 54 38196.0 55 35029.5 56 31540.0 57 28200.5 58 25494.0 59 22910.0 60 19393.5 61 15504.5 62 11876.0 63 8738.0 64 6184.0 65 4494.0 66 3215.0 67 2300.0 68 1647.5 69 1089.5 70 723.5 71 509.5 72 352.5 73 263.5 74 189.0 75 131.5 76 95.5 77 46.5 78 24.5 79 12.0 80 5.5 81 4.0 82 3.5 83 3.0 84 1.5 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02034445316961186 2 0.003776053807996141 3 3.0824929044866455E-4 4 0.0 5 0.002774243614037981 6 0.0 7 7.706232261216614E-5 8 0.0 9 0.0 10-11 0.0 12-13 0.004469614711505636 14-15 0.0031595552270988116 16-17 0.0 18-19 1.9265580653041536E-4 20-21 0.001155934839182492 22-23 0.001155934839182492 24-25 6.550297422034122E-4 26-27 2.6971812914258146E-4 28-29 7.706232261216614E-5 30-31 0.0 32-33 3.0824929044866455E-4 34-35 0.0 36-37 0.0012329971617946582 38-39 0.010441944713948511 40-41 0.001155934839182492 42-43 0.0 44-45 6.935609035094953E-4 46-47 1.5412464522433227E-4 48-49 2.3118696783649842E-4 50-51 0.0013100594844068245 52-53 3.4678045175474763E-4 54-55 0.0 56-57 1.1559348391824921E-4 58-59 1.1559348391824921E-4 60-61 0.002234807355752818 62-63 3.853116130608307E-5 64-65 7.706232261216614E-5 66-67 3.853116130608307E-5 68-69 1.5412464522433227E-4 70-71 3.0824929044866455E-4 72-73 2.3118696783649842E-4 74-75 0.0026586501301197316 76-77 1.9265580653041536E-4 78-79 1.1559348391824921E-4 80-81 0.001695371097467655 82-83 7.706232261216614E-5 84-85 0.002234807355752818 86-87 1.9265580653041536E-4 88-89 0.0023889320009771505 90-91 0.001155934839182492 92-93 0.0 94-95 0.0 96-97 1.1559348391824921E-4 98-99 3.0824929044866455E-4 100-101 0.002311869678364984 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1297651.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.08150587709081 #Duplication Level Percentage of deduplicated Percentage of total 1 80.29218101172813 49.04367326352311 2 11.929755310941784 14.573748382750917 3 3.4713110142334282 6.360987123513276 4 1.4632885513473197 3.5751947299600406 5 0.7494462689115897 2.2888653339543525 6 0.44665758091709407 1.6369510592300789 7 0.30962754094585054 1.3238761523395204 8 0.22113710731466552 1.0805910016066882 9 0.15017130519042232 0.8255420514503262 >10 0.8828069448807657 9.773116764393517 >50 0.05544860798035296 2.3235572468030483 >100 0.023981531353039175 2.5766959261586257 >500 0.0019032837525261052 0.7851117227066585 >1k 0.0019032837525261052 2.224896567002966 >5k 3.8065675050522106E-4 1.6071926746069078 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7684 0.5921468869518846 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7070 0.5448306208680146 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5983 0.46106387618859 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4211 0.3245094405198316 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3196 0.246291183068483 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2650 0.20421515492224027 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2123 0.1636033109056287 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2060 0.15874838458106225 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2015 0.15528058006351475 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1802 0.13886630534712338 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1592 0.1226832175985685 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1582 0.12191259437244684 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1542 0.11883010146796018 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1360 0.10480475875254594 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1331 0.10256995139679313 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 7.706232261216614E-5 0.0 0.0 0.0 0.0 6 7.706232261216614E-5 0.0 0.0 0.0 0.0 7 7.706232261216614E-5 0.0 0.0 0.0 0.0 8 7.706232261216614E-5 0.0 0.0 0.0 0.0 9 7.706232261216614E-5 0.0 0.0 0.0 0.0 10-11 7.706232261216614E-5 0.0 0.0 3.853116130608307E-5 0.0 12-13 7.706232261216614E-5 0.0 0.0 7.706232261216614E-5 0.0 14-15 2.3118696783649842E-4 0.0 0.0 7.706232261216614E-5 0.0 16-17 2.3118696783649842E-4 0.0 0.0 1.5412464522433227E-4 0.0 18-19 2.3118696783649842E-4 0.0 0.0 3.0824929044866455E-4 0.0 20-21 2.3118696783649842E-4 0.0 0.0 3.0824929044866455E-4 0.0 22-23 2.3118696783649842E-4 0.0 0.0 6.550297422034121E-4 0.0 24-25 3.8531161306083067E-4 0.0 0.0 6.935609035094953E-4 0.0 26-27 3.8531161306083067E-4 0.0 0.0 9.247478713459937E-4 0.0 28-29 4.623739356729968E-4 0.0 0.0 0.0015027152909372395 0.0 30-31 5.394362582851629E-4 0.0 0.0 0.003583398001465725 0.0 32-33 5.394362582851629E-4 0.0 0.0 0.0065117662607280385 0.0 34-35 5.394362582851629E-4 0.0 0.0 0.009825446133051183 0.0 36-37 5.394362582851629E-4 0.0 0.0 0.013062063682762162 0.0 38-39 5.394362582851629E-4 0.0 0.0 0.01533540219982106 0.0 40-41 5.394362582851629E-4 0.0 0.0 0.018225239297777293 0.0 42-43 6.164985808973291E-4 3.853116130608307E-5 0.0 0.021192138718345688 0.0 44-45 6.935609035094953E-4 7.706232261216614E-5 0.0 0.024775536719811413 0.0 46-47 7.706232261216613E-4 7.706232261216614E-5 0.0 0.03013136814135696 0.0 48-49 8.476855487338275E-4 7.706232261216614E-5 0.0 0.03698991485383975 0.0 50-51 8.476855487338275E-4 7.706232261216614E-5 0.0 0.04211455930754879 0.0 52-53 8.862167100399105E-4 2.3118696783649842E-4 0.0 0.0512079133757844 0.0 54-55 9.247478713459937E-4 2.3118696783649842E-4 0.0 0.06881665409266435 0.0 56-57 0.0010018101939581598 2.3118696783649842E-4 0.0 0.10888906185099076 0.0 58-59 0.0010018101939581598 2.3118696783649842E-4 0.0 0.15327695967559846 0.0 60-61 0.0010018101939581598 2.3118696783649842E-4 0.0 0.1922704949173545 0.0 62-63 0.0010018101939581598 2.3118696783649842E-4 0.0 0.24170597487305906 0.0 64-65 0.0010018101939581598 3.8531161306083067E-4 0.0 0.3005430581874479 0.0 66-67 0.0010018101939581598 4.2384277436691376E-4 0.0 0.38770054506180784 0.0 68-69 0.0010018101939581598 4.6237393567299685E-4 0.0 0.49944091284944875 0.0 70-71 0.0010018101939581598 4.6237393567299685E-4 0.0 0.5942275696624131 0.0 72-73 0.0010403413552642427 4.6237393567299685E-4 0.0 0.6979919870596948 0.0 74-75 0.0010788725165703258 4.6237393567299685E-4 0.0 0.8050700843292997 0.0 76-77 0.0010788725165703258 4.6237393567299685E-4 0.0 0.9307972636710486 0.0 78-79 0.0010788725165703258 4.6237393567299685E-4 0.0 1.0292443808080909 0.0 80-81 0.0013871218070189905 4.6237393567299685E-4 0.0 1.131814332204884 0.0 82-83 0.0014641841296311566 4.6237393567299685E-4 0.0 1.2442867920573404 0.0 84-85 0.0015027152909372395 4.6237393567299685E-4 0.0 1.427695119874296 0.0 86-87 0.0015797776135494058 4.6237393567299685E-4 0.0 1.6708267477156802 0.0 88-89 0.001618308774855489 4.6237393567299685E-4 0.0 1.944898898085849 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCACCG 165 2.4314159E-6 23.02907 5 GAGTACT 6630 0.0 22.102282 12-13 GTACATG 6855 0.0 21.831854 1 TACATGG 6925 0.0 21.399576 2 ACATGGG 6980 0.0 20.754618 3 GTACTTT 7095 0.0 20.75334 14-15 AGTACTT 6895 0.0 19.668322 12-13 AGAGTAC 11830 0.0 18.892035 10-11 GTATCAA 17890 0.0 18.085241 1 ACTTTTT 8490 0.0 17.482868 16-17 CATGGGA 5005 0.0 17.461603 4 CATGGGG 2380 0.0 17.362516 4 TACTTTT 8060 0.0 16.648003 14-15 TTAGACT 520 0.0 16.441427 4 CTTACAC 525 0.0 16.284842 3 AGACCCG 205 3.333812E-4 16.218643 5 CAACGCA 19820 0.0 16.152012 5 ATCAACG 19860 0.0 16.143396 3 TCAACGC 19860 0.0 16.143396 4 TATCAAC 20025 0.0 15.986659 2 >>END_MODULE