FastQCFastQC Report
Thu 26 May 2016
SRR936883_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936883_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577645
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77421.3402695427122195No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54510.9436591678279913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35470.6140449584087112No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16160.27975659791048135No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11450.1982186290888002No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9320.16134477057708455No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8670.1500921846462793No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC8290.1435137497944239No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8070.1397051822486129No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA7210.12481714547862442No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT6410.11096780894840257No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA6390.11062157553514702No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC5980.10352379056340832No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41050.038.3218651
GTATCAA67950.036.0904271
TAACGCC600.003938745231.684044
GACATAT1859.094947E-1230.8277131
TCAACGC83400.029.4046124
ATCAACG83450.029.3869933
TATCAAC83550.029.2949352
TTCGTCA650.005826054629.2468054
GTAGTAC650.005826054629.2468056
CAACGCA84750.028.880145
AACGCAG86650.028.2468786
ACCGTGC700.00836575427.1577479
GTACATG53800.026.7665351
TACATGG54550.026.2242772
CATGGGT7850.026.0333824
ACATGGG52900.025.7844583
ACGCAGA95300.025.3339317
CGCAGAG95500.025.3306438
ACGCAGC950.001221900325.0137167
GAGTACT48750.024.75478212-13
Produced by FastQC (version 0.11.5)