FastQCFastQC Report
Thu 26 May 2016
SRR936883_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936883_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577645
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92641.6037531701996903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70311.2171835642998727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48290.8359805763055164No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16960.2936059344407032No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16540.28633503276233674No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13380.23163015346796043No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12500.21639588328471637No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC10560.18281124219892841No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA8200.14195569943477396No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA7690.1331267473967575No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT7230.12516337889187998No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC7170.12412467865211332No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA6990.1210085779328134No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT6920.119796760986419No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6840.11841182733339682No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6610.11443014308095802No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5910.1023119736170139No Hit
TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT5830.10092703996399173No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45550.042.5557251
GTATCAA75800.038.108391
CTTGCGC500.001615519237.9982343
GGCGCGT250.001616532137.9965990-91
CGCGTTC352.052855E-433.9299392-93
TATCAAC95950.029.902192
ATCAACG97350.029.4696123
TCAACGC98100.029.2443084
CAACGCA99650.028.7894295
AACGCAG101900.028.2003576
TCTTGCG700.00838653427.1439462
GTACTAC700.00839007627.1415963
CATTCCG2007.185008E-1026.1215259
ACGCAGA114200.025.2024277
CGCAGAG114150.025.1718588
GCAGAGT116950.024.2443169
GGATATG3150.024.132131
GAGTACT71800.024.1106512-13
TAGGCAT2602.3646862E-1123.7488965
AGTACTT73350.023.69827812-13
Produced by FastQC (version 0.11.5)