FastQCFastQC Report
Thu 26 May 2016
SRR936882_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936882_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439575
Sequences flagged as poor quality0
Sequence length101
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63581.4463970880964567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44541.013251436046181No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29060.6610931012910197No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14160.32212932946596146No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9700.22066769038275608No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8370.19041119262924416No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7520.17107433316271398No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC6570.1494625490530626No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6570.1494625490530626No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA5750.13080816697946882No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA5190.11806858897799011No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT4820.10965136779844167No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4580.1041915486549508No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA4560.10373656372632657No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC4550.10350907126201446No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT4530.10305408633339021No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT4460.10146163908320537No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAT250.00500741757.0338973
GCGTATC250.00500741757.0338971
GTATCAA54400.036.345131
GGTATCA33200.034.357771
GTACAGA1401.6911144E-830.5538751
ATCAACG66250.029.7724133
TATCAAC66200.029.7231042
TCAACGC66800.029.527284
ATGGGCG650.005823248529.2481545
CAACGCA68000.029.006215
TAGGCAT2151.8189894E-1228.738015
AACGCAG68750.028.6832456
CATTCCG2053.092282E-1127.8182459
TTAGGCA2305.456968E-1226.8637944
GTACATG41650.026.474321
ACGCAGA75550.025.9128767
TACATGG42400.025.8939272
CGCAGAG76050.025.6829538
ATGGGGT5000.025.6652575
CATGGGT5200.025.5921344
Produced by FastQC (version 0.11.5)