##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936882_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 439575 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.970812716828757 33.0 31.0 34.0 30.0 34.0 2 32.05525336973213 33.0 31.0 34.0 30.0 34.0 3 32.03547972473412 33.0 31.0 34.0 30.0 34.0 4 35.65374054484445 37.0 35.0 37.0 33.0 37.0 5 35.67300915657169 37.0 35.0 37.0 35.0 37.0 6 35.7841096513678 37.0 35.0 37.0 35.0 37.0 7 35.74277427060229 37.0 35.0 37.0 35.0 37.0 8 35.69712108286413 37.0 35.0 37.0 33.0 37.0 9 37.33344707956549 39.0 37.0 39.0 34.0 39.0 10-11 37.172252744127846 39.0 37.0 39.0 34.0 39.0 12-13 37.114885969402266 39.0 37.0 39.0 33.5 39.0 14-15 38.19803901495763 40.0 38.0 41.0 33.0 41.0 16-17 38.166508559403965 40.0 38.0 41.0 33.0 41.0 18-19 38.26157993516465 40.0 38.0 41.0 33.5 41.0 20-21 38.256169026900984 40.0 38.0 41.0 34.0 41.0 22-23 38.1550304271171 40.0 38.0 41.0 34.0 41.0 24-25 37.98698174372974 40.0 38.0 41.0 33.0 41.0 26-27 37.98473411818233 40.0 38.0 41.0 33.0 41.0 28-29 37.89470056304385 40.0 37.0 41.0 33.0 41.0 30-31 37.630973099016096 40.0 37.0 41.0 33.0 41.0 32-33 37.287263834385485 39.5 35.5 41.0 33.0 41.0 34-35 37.07341409315816 39.0 35.0 41.0 32.5 41.0 36-37 36.837668202240806 39.0 35.0 41.0 31.5 41.0 38-39 36.60938178922824 39.0 35.0 41.0 31.0 41.0 40-41 36.37994881419553 39.0 35.0 41.0 30.5 41.0 42-43 36.019277711425815 39.0 35.0 41.0 30.0 41.0 44-45 35.51388955240858 39.0 35.0 41.0 28.0 41.0 46-47 34.80094295626458 38.5 35.0 40.0 24.0 41.0 48-49 34.17846783825286 38.0 34.5 40.0 17.5 41.0 50-51 33.36748450207587 38.0 33.5 39.5 11.0 40.5 52-53 33.51480179719047 38.0 34.0 40.0 8.5 40.5 54-55 33.84253085366547 39.0 34.0 40.5 6.5 41.0 56-57 33.74171643064324 39.0 34.0 41.0 2.0 41.0 58-59 33.50914405960303 38.0 34.0 41.0 2.0 41.0 60-61 33.22901552636069 38.0 34.0 40.0 2.0 41.0 62-63 32.905481430927594 38.0 33.5 40.0 2.0 41.0 64-65 32.620933856566 37.0 33.0 40.0 2.0 41.0 66-67 32.25358926235568 37.0 33.0 40.0 2.0 41.0 68-69 31.853310584086902 36.0 32.5 39.0 2.0 41.0 70-71 31.446477847921287 36.0 32.0 39.0 2.0 41.0 72-73 31.050120002274923 35.0 31.5 38.5 2.0 40.0 74-75 30.6117067622135 35.0 31.0 37.5 2.0 40.0 76-77 30.196067792754363 35.0 31.0 37.0 2.0 39.0 78-79 29.74750042654837 35.0 30.5 36.5 2.0 39.0 80-81 29.321963259967013 35.0 30.0 36.0 2.0 38.5 82-83 28.922852755502475 35.0 29.5 35.5 2.0 37.0 84-85 28.5678712392652 34.0 29.0 35.0 2.0 37.0 86-87 28.22861741454814 34.0 29.0 35.0 2.0 36.0 88-89 27.86875391002673 34.0 29.0 35.0 2.0 36.0 90-91 27.508168116931124 34.0 27.0 35.0 2.0 36.0 92-93 27.174910993573338 34.0 26.5 35.0 2.0 35.0 94-95 26.77098219871467 34.0 25.5 35.0 2.0 35.0 96-97 26.335392140135358 34.0 24.5 35.0 2.0 35.0 98-99 25.829804925211853 34.0 22.0 35.0 2.0 35.0 100-101 24.002021270545413 31.5 9.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22374813693623352 1101 2 0.3963593741104354 1101 3 0.17447986140564353 1101 4 0.1115440274996331 1101 5 0.24686430856569785 1101 6 0.12402117734867346 1101 7 0.11491494347701092 1101 8 0.19704913932947932 1101 9 0.24247972696279874 1101 10-11 0.314371089563231 1101 12-13 0.2936443855596167 1101 14-15 0.5828760032033315 1101 16-17 0.6065430786390991 1101 18-19 0.27686074912171676 1101 20-21 0.21092561853049574 1101 22-23 0.461357360284552 1101 24-25 0.5182412191003536 1101 26-27 0.5599397685073058 1101 28-29 0.5729251561922126 1101 30-31 0.581009217143567 1101 32-33 0.9309617784080828 1101 34-35 0.7973363323439742 1101 36-37 0.6461844237007384 1101 38-39 0.7505657494174471 1101 40-41 0.8747967460254884 1101 42-43 0.9784218836903946 1101 44-45 1.0965134909832202 1101 46-47 1.0773880779336622 1101 48-49 1.4650639100705902 1101 50-51 1.4488380808589696 1101 52-53 1.6934027808416445 1101 54-55 1.5586586148415265 1101 56-57 1.7521538861022563 1101 58-59 1.6271236064967596 1101 60-61 1.678229184379937 1101 62-63 1.6448095639879625 1101 64-65 1.6704466211599538 1101 66-67 1.8718059890795935 1101 68-69 2.0367694375071075 1101 70-71 2.002685370131214 1101 72-73 2.0607722854789188 1101 74-75 1.6706357483326677 1101 76-77 1.7266432233937685 1101 78-79 2.030262363594339 1101 80-81 1.7733375899997306 1101 82-83 1.5470093659055912 1101 84-85 1.3419044366735982 1101 86-87 1.3779550385753723 1101 88-89 1.5917423593504942 1101 90-91 1.581115129587122 1101 92-93 1.75796788218452 1101 94-95 1.7732872824435866 1101 96-97 1.6703102495389572 1101 98-99 1.713156984172862 1101 100-101 1.811088792249322 1102 1 0.4535147705012754 1102 2 0.42455368063145116 1102 3 0.4591207088071698 1102 4 0.39661519598688244 1102 5 0.3008034015812555 1102 6 0.11971796649133637 1102 7 0.34306787297055763 1102 8 0.3541659880550725 1102 9 0.47039266969699156 1102 10-11 0.5821507982689482 1102 12-13 0.5517004928088696 1102 14-15 0.7012225771854546 1102 16-17 0.7338270905556854 1102 18-19 0.5714486339280782 1102 20-21 0.603312245475216 1102 22-23 0.3420094622375487 1102 24-25 0.7087079519702613 1102 26-27 0.581749599689033 1102 28-29 0.5034903663875099 1102 30-31 0.4996909450482363 1102 32-33 0.6653171243200333 1102 34-35 0.6322585435323234 1102 36-37 0.4689252380006437 1102 38-39 0.7076163948990768 1102 40-41 0.44405775231479794 1102 42-43 0.7137537274507366 1102 44-45 0.8883787673817665 1102 46-47 0.7759522950803017 1102 48-49 1.0355000239401733 1102 50-51 1.260775353285318 1102 52-53 1.0261816620068274 1102 54-55 1.1065111471156044 1102 56-57 1.3816267427722337 1102 58-59 1.1592818322498246 1102 60-61 1.0504734743501487 1102 62-63 1.2102887099661146 1102 64-65 1.4218368892830924 1102 66-67 0.9689054594536408 1102 68-69 0.9543174348589822 1102 70-71 0.9918983790686475 1102 72-73 1.2440849302985129 1102 74-75 1.169187552371067 1102 76-77 1.2065753650687085 1102 78-79 1.2257564053023806 1102 80-81 1.4222079971496804 1102 82-83 1.2175158207218786 1102 84-85 1.349133003373364 1102 86-87 1.291357057774313 1102 88-89 1.3566670531604892 1102 90-91 1.3237086416692136 1102 92-93 1.4219905567955742 1102 94-95 1.4925234625164094 1102 96-97 1.7502713551331297 1102 98-99 1.6477274906276804 1102 100-101 1.3810118309782204 1103 1 0.5067670048607589 1103 2 0.3837807577582346 1103 3 0.4656748299591058 1103 4 0.42286478221661383 1103 5 0.2308265727166443 1103 6 0.3560966490467834 1103 7 0.2878709183197756 1103 8 0.347363604738284 1103 9 0.32141054457286344 1103 10-11 0.43496857383996 1103 12-13 0.38609721574830047 1103 14-15 0.5302030472284898 1103 16-17 0.646008487444135 1103 18-19 0.5078355918890125 1103 20-21 0.6728753040650872 1103 22-23 0.5410743414166319 1103 24-25 0.4455997096663893 1103 26-27 0.5038076930356112 1103 28-29 0.5322018857519595 1103 30-31 0.16009726745803476 1103 32-33 0.4386661809238106 1103 34-35 0.39529230718674313 1103 36-37 0.12700203376363817 1103 38-39 0.13924499470046214 1103 40-41 0.05529989067957786 1103 42-43 0.27527722960234513 1103 44-45 0.3245009123668652 1103 46-47 0.3396522288770569 1103 48-49 0.2971393817687087 1103 50-51 0.2964795902929396 1103 52-53 0.4193461770680571 1103 54-55 0.29026238842642726 1103 56-57 0.29036143327206787 1103 58-59 0.4151739209621681 1103 60-61 0.5441096875246032 1103 62-63 0.49701082184959944 1103 64-65 0.5610126588957982 1103 66-67 0.5667745425387132 1103 68-69 0.5686876765008257 1103 70-71 0.6135344267349829 1103 72-73 0.6827848640952681 1103 74-75 0.6604156225465054 1103 76-77 0.8639073743371632 1103 78-79 0.7838787157955984 1103 80-81 0.6110734390563337 1103 82-83 0.6941791772263457 1103 84-85 0.6683195310132213 1103 86-87 0.5516971769401522 1103 88-89 0.4463021077781626 1103 90-91 0.601240915750644 1103 92-93 0.6892571903606175 1103 94-95 0.5567778484813246 1103 96-97 0.7927945262685157 1103 98-99 1.025263902411858 1103 100-101 1.1312145784128447 1104 1 0.54657462602243 1104 2 0.3793782420349636 1104 3 0.5301217407989114 1104 4 0.37576896275675153 1104 5 0.3319179526648455 1104 6 0.31858277557286385 1104 7 0.4702608554266945 1104 8 0.4195426650416536 1104 9 0.6055577883760392 1104 10-11 0.6040677229335571 1104 12-13 0.6341179334693479 1104 14-15 0.8865940942133221 1104 16-17 0.8974235817837553 1104 18-19 0.7911596762397366 1104 20-21 0.8073370132770705 1104 22-23 0.6726596170363166 1104 24-25 0.9294509860259907 1104 26-27 0.7040019216704678 1104 28-29 0.7057220485340565 1104 30-31 0.8704468790007596 1104 32-33 0.8817386929474864 1104 34-35 0.9631150966798927 1104 36-37 0.8638684784547195 1104 38-39 0.9770178396875195 1104 40-41 0.9330283405500879 1104 42-43 0.684045265118435 1104 44-45 0.8718926992703118 1104 46-47 1.3185090546262188 1104 48-49 1.606462356241515 1104 50-51 1.6834662717579718 1104 52-53 1.689055759014046 1104 54-55 1.7192949448452168 1104 56-57 1.8649729760023703 1104 58-59 1.8508194999484786 1104 60-61 1.9344260027299285 1104 62-63 1.7705957275099706 1104 64-65 1.8716304909342796 1104 66-67 1.751735696811739 1104 68-69 1.7442426154575408 1104 70-71 1.6348163357249952 1104 72-73 1.8253671800405158 1104 74-75 1.7461999362719958 1104 76-77 1.6101615363793407 1104 78-79 1.850795104992784 1104 80-81 1.6910031467884856 1104 82-83 1.7821185039004135 1104 84-85 1.9289188650864695 1104 86-87 2.108088223924984 1104 88-89 2.318083461829218 1104 90-91 2.371773657149088 1104 92-93 2.4012346228430452 1104 94-95 2.089620985736225 1104 96-97 2.0826113964128012 1104 98-99 2.139794054095173 1104 100-101 1.827773956881213 1105 1 0.08208033909902923 1105 2 0.2812967072627792 1105 3 0.024676685078780736 1105 4 0.3543837180113698 1105 5 0.0879484224067042 1105 6 0.1093431084830101 1105 7 0.10695150290871425 1105 8 0.23689349026383155 1105 9 0.2782337652416089 1105 10-11 0.18400142303230638 1105 12-13 0.24933869803417252 1105 14-15 0.20443158892343405 1105 16-17 0.01480967291085733 1105 18-19 0.15498391096959807 1105 20-21 0.22557653930331156 1105 22-23 0.33112343421785795 1105 24-25 0.5284353279642104 1105 26-27 0.4844363976191133 1105 28-29 0.5930192695807506 1105 30-31 -0.0893993616537685 1105 32-33 0.1767379876293873 1105 34-35 0.327891510842683 1105 36-37 0.25447364138319983 1105 38-39 0.16512617654744588 1105 40-41 0.35008293217091335 1105 42-43 0.08322709612421875 1105 44-45 0.3515019530437584 1105 46-47 -0.18276757113198983 1105 48-49 -0.0907163713646355 1105 50-51 -0.4416148398233517 1105 52-53 -0.5673908484058998 1105 54-55 -0.32413854365809414 1105 56-57 -0.06148350587095308 1105 58-59 -0.22262527364440388 1105 60-61 -0.10143236939590139 1105 62-63 -0.29295343316148603 1105 64-65 -0.20338714201817965 1105 66-67 -0.19224088662738836 1105 68-69 -0.17897645634384318 1105 70-71 0.015060375787061986 1105 72-73 -0.05546227404327908 1105 74-75 -0.22117910167412091 1105 76-77 -0.13934474095173854 1105 78-79 -0.07641606724589423 1105 80-81 -0.11364489948038425 1105 82-83 -0.029928329221327488 1105 84-85 0.2287919078287466 1105 86-87 0.21393531096123652 1105 88-89 0.16320971376884685 1105 90-91 0.3852099669247977 1105 92-93 -0.024290839140309828 1105 94-95 0.013312620663576524 1105 96-97 0.1550122992199654 1105 98-99 0.4271970728566359 1105 100-101 0.8955691325054183 1106 1 0.08080310002072721 1106 2 0.23282027102046854 1106 3 0.1771699078918374 1106 4 0.35551107699374995 1106 5 0.1602427608489876 1106 6 0.08299027770415535 1106 7 0.14870288441220225 1106 8 0.2861453930893916 1106 9 0.4952210449830332 1106 10-11 0.5973967936922975 1106 12-13 0.5947210877745519 1106 14-15 0.607358160709488 1106 16-17 0.27550329192867906 1106 18-19 0.38207506413402115 1106 20-21 0.4821451974203015 1106 22-23 0.22523314644807613 1106 24-25 0.332392162496582 1106 26-27 0.3825505150208457 1106 28-29 0.46803556089551535 1106 30-31 0.2932836223937727 1106 32-33 0.4854258199722068 1106 34-35 0.3793981316330033 1106 36-37 0.768067114430842 1106 38-39 0.9159830997045688 1106 40-41 1.071775143619142 1106 42-43 0.7675437732173265 1106 44-45 0.8165538245900095 1106 46-47 0.7319734621283516 1106 48-49 1.0261607790889187 1106 50-51 1.0466163143837193 1106 52-53 1.083961982372962 1106 54-55 1.1227582867037071 1106 56-57 1.2751765139392788 1106 58-59 1.4150884684054361 1106 60-61 1.335699446890196 1106 62-63 1.653295002872298 1106 64-65 1.404660115827717 1106 66-67 1.3582361230692008 1106 68-69 1.5006418055683675 1106 70-71 1.4328022692288265 1106 72-73 1.2598887060422186 1106 74-75 1.4792562022566464 1106 76-77 1.4544443582037196 1106 78-79 1.3379274579339615 1106 80-81 1.3225941527560856 1106 82-83 1.6843350426898382 1106 84-85 1.552565497652541 1106 86-87 1.6134537396965065 1106 88-89 1.5865297533893212 1106 90-91 1.7909712274815703 1106 92-93 1.7167130968413424 1106 94-95 1.310401036887118 1106 96-97 1.1325368013573858 1106 98-99 0.9211399620235596 1106 100-101 0.8288236158752476 1107 1 -0.06364068626051989 1107 2 0.08628105765712135 1107 3 -0.16028270435789693 1107 4 0.12376276518235585 1107 5 0.009831079768552797 1107 6 -0.045519377549965157 1107 7 -0.10240036164869082 1107 8 -0.12276646569510774 1107 9 -0.1022052078306146 1107 10-11 0.014868064868700515 1107 12-13 -0.04251827959898691 1107 14-15 -0.026989813464190604 1107 16-17 0.2751256707937415 1107 18-19 -0.061944453695609525 1107 20-21 0.13926520689784638 1107 22-23 -0.32696372003693597 1107 24-25 -0.2541200990837993 1107 26-27 0.09861644322840846 1107 28-29 -0.1797216657363947 1107 30-31 0.10613903052077234 1107 32-33 0.2038389226851578 1107 34-35 1.7766012489062177E-4 1107 36-37 0.06499579586670023 1107 38-39 -0.4862241328508219 1107 40-41 -0.04044497248084866 1107 42-43 0.11386307780208682 1107 44-45 0.40674604115202584 1107 46-47 0.42759972086547293 1107 48-49 0.4047259668628769 1107 50-51 0.29488369976459694 1107 52-53 0.18692667849901312 1107 54-55 0.31120604170904187 1107 56-57 0.11084838338603475 1107 58-59 0.10337332801922372 1107 60-61 0.02164083416658258 1107 62-63 0.05306620889054159 1107 64-65 0.21028518987107248 1107 66-67 -0.04158321113290597 1107 68-69 -0.0729609962323714 1107 70-71 -0.3791201925647947 1107 72-73 -0.22381805263566434 1107 74-75 -0.1984626699148926 1107 76-77 0.0167037172843969 1107 78-79 0.07302623177592338 1107 80-81 0.08097215891649512 1107 82-83 -0.2494998110004829 1107 84-85 -0.15779909185107144 1107 86-87 -0.1194829680395415 1107 88-89 -0.31228999545560043 1107 90-91 -0.42983717792063203 1107 92-93 -0.5843212888949445 1107 94-95 -0.6535990815822572 1107 96-97 -0.6315388413389762 1107 98-99 -0.5519193615163047 1107 100-101 -0.30470988134072385 1108 1 0.45585176971573205 1108 2 0.15002500820411413 1108 3 0.35362188712609566 1108 4 0.5235594222241176 1108 5 0.37924016513192527 1108 6 0.19230241268144255 1108 7 0.09165389025256587 1108 8 0.3926184625248297 1108 9 0.09692448430814693 1108 10-11 0.4376790779233133 1108 12-13 0.5555987646077725 1108 14-15 0.6294825928963732 1108 16-17 0.715582785763857 1108 18-19 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-1.3554490798516277 2316 80-81 -1.5153151274456391 2316 82-83 -1.2019643383753404 2316 84-85 -1.4334368329045013 2316 86-87 -2.0059801450746377 2316 88-89 -1.7689422799261827 2316 90-91 -1.2962455716320562 2316 92-93 -1.5585003783630604 2316 94-95 -2.2196574180681807 2316 96-97 -1.9710344907645663 2316 98-99 -1.8949107944523291 2316 100-101 -2.0343301817865544 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 748.0 3 259.0 4 238.0 5 236.0 6 283.0 7 377.0 8 460.0 9 589.0 10 838.0 11 1250.0 12 1859.0 13 2711.0 14 3213.0 15 4558.0 16 6572.0 17 10027.0 18 8448.0 19 4057.0 20 3237.0 21 2910.0 22 2961.0 23 3132.0 24 3428.0 25 3804.0 26 4266.0 27 5143.0 28 6025.0 29 7038.0 30 8620.0 31 10475.0 32 13466.0 33 17928.0 34 25297.0 35 35905.0 36 51985.0 37 81402.0 38 88716.0 39 17110.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.483234978846006 20.936874853492398 15.403154778025337 19.176735389636253 2 15.093605341854083 22.674659420388803 41.369555619279105 20.86217961847801 3 18.117216600641218 29.84911610846529 32.11863191930408 19.91503537158941 4 11.514257687589472 19.431831889243178 40.29910532412939 28.754805099037956 5 11.15881391505461 40.77155617877777 33.828142428533056 14.241487477634568 6 25.554306058895303 40.44294403671395 19.27209484438455 14.730655060006193 7 24.714811585558802 38.22993095378479 21.381789373757883 15.67346808689852 8 26.27543470639391 33.92030268951105 20.514635912052487 19.289626692042564 9 28.803542361498447 16.752911179155618 21.592924383330867 32.85062207601507 10-11 23.586970750781173 28.989453434037003 27.35299976664371 20.070576048538108 12-13 26.112098276943463 27.89423299112006 27.868393403733876 18.1252753282026 14-15 23.175272424277264 27.676576349941552 29.054150194479007 20.094001031302184 16-17 19.098720750250386 31.267640899572065 28.94359464627151 20.690043703906035 18-19 19.07424330189742 28.54097840151402 34.43353335935606 17.951244937232495 20-21 20.875538903482216 26.97453780632708 34.30499091774068 17.844932372450025 22-23 20.62895529112991 27.087699656155635 33.956167238982076 18.327177813732384 24-25 19.508404852698927 27.74090574005479 34.60847129062497 18.142218116621315 26-27 19.224551875711704 27.756119369366804 34.80688877689968 18.212439978021802 28-29 18.775056084675345 28.217986230245224 34.824374195133714 18.182583489945713 30-31 19.647281521810847 27.813409354104053 34.65937401172619 17.87993511235891 32-33 19.061740697261733 28.075887047603448 34.34198312812253 18.520389127012294 34-35 19.328076190649536 28.36991415811016 34.21025688862396 18.091752762616345 36-37 19.680627188322575 27.865493949480037 34.24726253500747 18.206616327189913 38-39 19.132516963468113 28.851034302322276 33.82046308988221 18.195985644327408 40-41 19.11440843775879 28.69470456013905 33.8120068797947 18.37888012230746 42-43 18.91407069358152 29.018630036013366 33.005694343648976 19.061604926756136 44-45 19.484157903417525 28.88852237116744 32.7742295070779 18.853090218337133 46-47 19.523461778208507 29.143746331608906 31.990531569775644 19.342260320406947 48-49 19.58061525686889 29.66677851776442 31.478197566954258 19.274408658412433 50-51 19.810421472674573 29.883131537877027 30.707376113060057 19.59907087638834 52-53 19.81356250319924 30.382259431359948 30.252583564152584 19.55159450128823 54-55 19.651618201942227 29.84234887548871 30.280414017629536 20.225618904939523 56-57 20.098232184750234 30.374497404822637 29.46908655673096 20.058183853696164 58-59 20.141103184832204 30.16052136739497 29.750202770418383 19.94817267735444 60-61 20.535486748216105 30.03790774719674 30.076930428134556 19.3496750764526 62-63 20.184451639178285 29.829085887072004 30.29971400553787 19.68674846821184 64-65 19.91923282559978 29.729828113802085 30.463785591591115 19.887153469007018 66-67 20.1794293346257 30.08467490093958 29.586955309900127 20.148940454534596 68-69 19.8309101521035 29.78925775450229 30.073235859972396 20.30659623342181 70-71 19.839632848416862 30.140514662968453 30.111048791614852 19.908803696999826 72-73 19.893832211207005 30.088875135667692 29.926984047527494 20.090308605597816 74-75 19.384201622545902 30.670463452898726 29.724148993900947 20.22118593065442 76-77 19.21660894614926 30.3788164942636 29.865475545766074 20.53909901382107 78-79 19.238524384970756 30.41146082246649 29.57454313738877 20.775471655173984 80-81 19.37353866458832 30.479403348484762 29.382470232846554 20.76458775408036 82-83 19.53190956775023 30.64991068279307 29.285121004903914 20.533058744552786 84-85 19.410636395284545 30.076156280788457 29.115613289009328 21.397594034917667 86-87 19.294774578390424 30.090459899448547 29.27039329748007 21.344372224680956 88-89 19.552401473489255 29.25317576069224 29.659318181301064 21.53510458451744 90-91 19.160256971787483 29.246183477492217 30.177006553986484 21.416552996733813 92-93 19.603479368980366 29.52366323622276 29.908123268422283 20.964734126374594 94-95 19.127603440802474 30.162085414815564 29.478111586029733 21.232199558352228 96-97 19.32971713302906 30.41943599564417 28.650137741046834 21.60070913027993 98-99 18.95484643417258 30.505573348083303 28.380826863150805 22.158753354593316 100-101 19.583460907630897 30.38529524581338 28.67536334487857 21.355880501677152 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 780.0 1 546.0 2 337.0 3 375.0 4 345.0 5 357.0 6 545.5 7 972.0 8 1593.0 9 1872.5 10 1563.5 11 1256.0 12 1234.5 13 1282.5 14 1323.0 15 1338.0 16 1382.0 17 1529.5 18 1680.0 19 1801.5 20 2002.0 21 2241.5 22 2460.5 23 2729.0 24 3063.0 25 3689.5 26 5018.5 27 6351.0 28 6747.0 29 7140.5 30 8090.5 31 9087.0 32 10569.0 33 12292.0 34 14428.5 35 16130.0 36 17779.0 37 19208.0 38 17758.0 39 15883.0 40 15040.5 41 14307.0 42 13798.0 43 13968.5 44 14424.5 45 14135.5 46 13392.5 47 12940.0 48 14329.0 49 14214.0 50 12220.5 51 10839.5 52 9410.5 53 8536.5 54 7735.0 55 6914.5 56 6291.0 57 5782.0 58 5067.0 59 4400.0 60 4037.0 61 3661.0 62 3019.0 63 2027.5 64 1252.0 65 882.0 66 626.5 67 465.0 68 362.0 69 272.5 70 200.5 71 154.0 72 118.0 73 107.5 74 86.5 75 58.0 76 39.5 77 22.5 78 15.5 79 8.0 80 3.5 81 3.0 82 4.0 83 3.0 84 2.0 85 1.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 1.5 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0404936586475573 2 0.04026616618324518 3 0.022294261502587728 4 0.045043507933799694 5 0.06392538247170562 6 0.06529033725757834 7 0.06915770915088437 8 0.07120514132969345 9 0.0727975885798783 10-11 0.07564124438377978 12-13 0.07427628959790707 14-15 0.07382130466928283 16-17 0.058010578399590514 18-19 0.049024626059261785 20-21 0.05846556332821475 22-23 0.030029005289199794 24-25 0.015241995108912018 26-27 0.011033384519137804 28-29 0.0138770403230393 30-31 0.010464653358357504 32-33 0.006142296536427231 34-35 0.010464653358357504 36-37 0.005914804072115111 38-39 0.0062560427685832915 40-41 0.006142296536427231 42-43 0.0036398794289939146 44-45 5.687311607802992E-4 46-47 0.002047432178809077 48-49 5.687311607802992E-4 50-51 0.004777341750554513 52-53 0.003981118125462094 54-55 0.006483535232895411 56-57 0.024114201217084685 58-59 0.008758459876016608 60-61 0.019791844395154412 62-63 0.0127395780014787 64-65 0.009099698572484787 66-67 0.016038218734004437 68-69 0.023772962520616506 70-71 0.01899562077006199 72-73 0.018654382073593812 74-75 0.018313143377125633 76-77 0.022976738895524085 78-79 0.037536256611499744 80-81 0.027867826878234657 82-83 0.02957402036057556 84-85 0.01910936700221805 86-87 0.022408007734743787 88-89 0.016834442359096854 90-91 0.016265711198316556 92-93 0.029005289199795257 94-95 0.01922311323437411 96-97 0.03719501791503156 98-99 0.05732810100665416 100-101 0.06563157595404652 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 439575.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.102401464185476 #Duplication Level Percentage of deduplicated Percentage of total 1 83.8353643977371 44.51859177144917 2 9.057450104990638 9.619447034340835 3 2.4338962322186304 3.8773720453632636 4 1.1036489538240388 2.3442563928596964 5 0.655851837364595 1.7413653784379202 6 0.42796773919149655 1.3635668820160005 7 0.31828989660118423 1.183137050991712 8 0.2397322688813444 1.0184287348845769 9 0.19744997781654802 0.9436561191997964 >10 1.493811699397617 15.757130921940812 >50 0.1493917212489039 5.387869344246786 >100 0.08242301861954882 7.662924815144791 >500 0.003005005887171051 1.132284621651768 >1k 0.0012878596659304502 2.0005898610058215 >5k 4.2928655531015005E-4 1.4493790264670707 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6358 1.4463970880964567 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4454 1.013251436046181 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2906 0.6610931012910197 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1416 0.32212932946596146 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 970 0.22066769038275608 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 837 0.19041119262924416 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 752 0.17107433316271398 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 657 0.1494625490530626 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 657 0.1494625490530626 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 575 0.13080816697946882 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 519 0.11806858897799011 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 482 0.10965136779844167 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 458 0.1041915486549508 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 456 0.10373656372632657 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 455 0.10350907126201446 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 453 0.10305408633339021 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 446 0.10146163908320537 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.1374623215605983E-4 0.0 16-17 0.0 0.0 0.0 6.82477392936359E-4 0.0 18-19 0.0 0.0 0.0 6.82477392936359E-4 0.0 20-21 0.0 0.0 0.0 9.099698572484786E-4 0.0 22-23 0.0 0.0 0.0 0.0017061934823408976 0.0 24-25 0.0 0.0 0.0 0.0025024171074333164 0.0 26-27 0.0 0.0 0.0 0.004436103054086334 0.0 28-29 0.0 0.0 0.0 0.009327191036796906 0.0 30-31 0.0 0.0 0.0 0.015014502644599897 0.0 32-33 0.0 0.0 0.0 0.022976738895524085 0.0 34-35 0.0 0.0 0.0 0.029232781664107376 0.0 36-37 0.0 0.0 0.0 0.03696752545071945 0.0 38-39 0.0 0.0 0.0 0.04822840243416937 0.0 40-41 0.0 0.0 0.0 0.07313882727634646 0.0 42-43 0.0 0.0 0.0 0.1192060512995507 0.0 44-45 0.0 0.0 0.0 0.15856224762554738 0.0 46-47 0.0 0.0 0.0 0.2541090826366377 0.0 48-49 0.0 0.0 0.0 0.3456747995222658 0.0 50-51 0.0 0.0 0.0 0.4091451970653472 0.0 52-53 0.0 0.0 0.0 0.48706136609224815 0.0 54-55 0.0 0.0 0.0 0.5406358414377523 0.0 56-57 0.0 0.0 0.0 0.7133026218506513 0.0 58-59 0.0 0.0 0.0 0.9322641187510663 0.0 60-61 0.0 0.0 0.0 1.1420121708468407 0.0 62-63 0.0 0.0 0.0 1.4156856054143208 0.0 64-65 0.0 0.0 0.0 1.7740999829380653 0.0 66-67 0.0 0.0 0.0 2.217027810953762 0.0 68-69 0.0 0.0 0.0 2.714667576636524 0.0 70-71 0.0 0.0 0.0 3.0476027981573113 0.0 72-73 0.0 0.0 0.0 3.3243473809930046 0.0 74-75 0.0 0.0 0.0 3.6865153841778993 0.0 76-77 0.0 0.0 0.0 4.147301370642097 0.0 78-79 0.0 0.0 0.0 4.529033725757834 0.0 80-81 0.0 0.0 0.0 4.921230734231928 0.0 82-83 0.0 0.0 0.0 5.462094068133993 0.0 84-85 0.0 0.0 0.0 6.477279190126827 0.0 86-87 0.0 0.0 0.0 7.645566740601717 0.0 88-89 0.0 0.0 0.0 8.688960928169255 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTCGAT 25 0.005007417 57.033897 3 GCGTATC 25 0.005007417 57.033897 1 GTATCAA 5440 0.0 36.34513 1 GGTATCA 3320 0.0 34.35777 1 GTACAGA 140 1.6911144E-8 30.553875 1 ATCAACG 6625 0.0 29.772413 3 TATCAAC 6620 0.0 29.723104 2 TCAACGC 6680 0.0 29.52728 4 ATGGGCG 65 0.0058232485 29.248154 5 CAACGCA 6800 0.0 29.00621 5 TAGGCAT 215 1.8189894E-12 28.73801 5 AACGCAG 6875 0.0 28.683245 6 CATTCCG 205 3.092282E-11 27.818245 9 TTAGGCA 230 5.456968E-12 26.863794 4 GTACATG 4165 0.0 26.47432 1 ACGCAGA 7555 0.0 25.912876 7 TACATGG 4240 0.0 25.893927 2 CGCAGAG 7605 0.0 25.682953 8 ATGGGGT 500 0.0 25.665257 5 CATGGGT 520 0.0 25.592134 4 >>END_MODULE