##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936882_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 439575 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.131590741056705 33.0 31.0 34.0 31.0 34.0 2 32.182603651254055 33.0 31.0 34.0 30.0 34.0 3 32.27944491838708 34.0 31.0 34.0 30.0 34.0 4 36.02029915259057 37.0 35.0 37.0 35.0 37.0 5 35.951623727464025 37.0 35.0 37.0 35.0 37.0 6 36.00677472558721 37.0 35.0 37.0 35.0 37.0 7 35.95600750725132 37.0 35.0 37.0 35.0 37.0 8 35.95259057043735 37.0 35.0 37.0 35.0 37.0 9 37.60012284593073 39.0 37.0 39.0 35.0 39.0 10-11 37.517097196155376 39.0 37.0 39.0 35.0 39.0 12-13 37.4317488483194 39.0 37.0 39.0 34.5 39.0 14-15 38.504545299436955 40.0 38.0 41.0 34.0 41.0 16-17 38.45813228686799 40.0 38.0 41.0 34.0 41.0 18-19 38.628178354092015 40.0 38.0 41.0 34.5 41.0 20-21 38.75063299778195 40.0 38.0 41.0 35.0 41.0 22-23 38.66605243701302 40.0 38.0 41.0 34.0 41.0 24-25 38.562939202638916 40.0 38.0 41.0 34.0 41.0 26-27 38.344212022976734 40.0 38.0 41.0 34.0 41.0 28-29 38.1352624694307 40.0 37.0 41.0 34.0 41.0 30-31 37.83555479724734 40.0 37.0 41.0 33.0 41.0 32-33 37.51333902064494 40.0 35.5 41.0 33.0 41.0 34-35 37.26791673775806 39.5 35.0 41.0 33.0 41.0 36-37 36.9802377296252 39.0 35.0 41.0 33.0 41.0 38-39 36.723263379400564 39.0 35.0 41.0 32.0 41.0 40-41 36.367746118409826 39.0 35.0 41.0 31.0 41.0 42-43 35.93251549792413 39.0 35.0 41.0 30.0 41.0 44-45 35.300892907922425 39.0 35.0 41.0 27.5 41.0 46-47 34.4021099926065 39.0 35.0 41.0 19.0 41.0 48-49 33.61012682704886 38.5 34.0 40.0 4.0 41.0 50-51 33.20195529773076 38.0 34.0 40.0 2.0 41.0 52-53 32.92709776488654 38.0 33.0 40.0 2.0 41.0 54-55 32.640031848945 38.0 33.0 40.0 2.0 41.0 56-57 32.39669453449355 38.0 33.0 40.0 2.0 41.0 58-59 32.072206108172665 38.0 32.5 40.0 2.0 41.0 60-61 31.75561280782574 37.0 32.0 40.0 2.0 41.0 62-63 31.42551782972189 36.5 31.0 40.0 2.0 41.0 64-65 31.048445657737588 36.0 31.0 39.0 2.0 41.0 66-67 30.639018370016494 36.0 30.5 39.0 2.0 40.0 68-69 30.192736165614512 35.0 30.0 38.5 2.0 40.0 70-71 29.807613035318205 35.0 29.0 38.0 2.0 40.0 72-73 29.430612523460162 35.0 29.0 37.5 2.0 39.5 74-75 28.9617778536086 34.5 28.5 37.0 2.0 39.0 76-77 27.5572882898254 33.0 26.5 35.5 2.0 37.5 78-79 28.171648751635104 34.0 27.5 36.0 2.0 38.0 80-81 27.790265597452084 34.0 26.0 35.5 2.0 37.0 82-83 27.62790763805949 34.0 26.0 35.0 2.0 37.0 84-85 27.327264971847807 34.0 26.0 35.0 2.0 36.5 86-87 26.987122789057615 34.0 26.0 35.0 2.0 36.0 88-89 26.617239378945573 34.0 25.0 35.0 2.0 36.0 90-91 26.208559403969744 33.0 24.5 35.0 2.0 35.0 92-93 25.82180060285503 33.0 22.5 35.0 2.0 35.0 94-95 25.3846795199909 33.0 19.5 35.0 2.0 35.0 96-97 24.84314735824376 32.5 12.0 35.0 2.0 35.0 98-99 24.199630324745492 32.0 2.0 34.0 2.0 35.0 100-101 21.529662742421657 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3650243327147109 1101 2 0.34504507799893247 1101 3 0.3168945165084409 1101 4 0.215334998387128 1101 5 0.21978136517814306 1101 6 0.24694647323468644 1101 7 0.27188940993869437 1101 8 0.2899238274409299 1101 9 0.24854436469176022 1101 10-11 0.29389506414486277 1101 12-13 0.38025764510809523 1101 14-15 0.40984736341827954 1101 16-17 0.3904540780827972 1101 18-19 0.29780905455927353 1101 20-21 0.3169190140910345 1101 22-23 0.38925149189788755 1101 24-25 0.26686168979802005 1101 26-27 0.26567029341318715 1101 28-29 0.3379539300288812 1101 30-31 0.3667259704923893 1101 32-33 0.6318644782340357 1101 34-35 0.5912149193114757 1101 36-37 0.6211101859143895 1101 38-39 0.6544889936116789 1101 40-41 0.862928150374124 1101 42-43 0.9228400040580098 1101 44-45 1.1276646145820095 1101 46-47 1.37520181352658 1101 48-49 1.4814906882839693 1101 50-51 1.43561859046374 1101 52-53 1.3815573438971214 1101 54-55 1.4994908205934863 1101 56-57 1.550462340069842 1101 58-59 1.5904394892068048 1101 60-61 1.5604468396366435 1101 62-63 1.4194469915766597 1101 64-65 1.6632590980184965 1101 66-67 1.673802938016518 1101 68-69 1.5511262447922398 1101 70-71 1.7031317239764725 1101 72-73 1.4367100107234307 1101 74-75 1.4496207780420711 1101 76-77 1.3514739127811701 1101 78-79 1.5184237630655026 1101 80-81 1.9121142535869957 1101 82-83 1.7078745183795228 1101 84-85 1.720886533188633 1101 86-87 1.6249397132939905 1101 88-89 1.654466686348922 1101 90-91 1.4067494036643176 1101 92-93 1.550801106798609 1101 94-95 1.8471316776804905 1101 96-97 1.695442873424085 1101 98-99 1.9205363347150417 1101 100-101 1.8202168104760084 1102 1 0.3105887153694624 1102 2 0.26248963278212045 1102 3 0.2160669759589524 1102 4 0.21007184049238958 1102 5 0.28244273558529187 1102 6 0.3191576615829135 1102 7 0.24282618584071258 1102 8 0.05246106676565887 1102 9 0.3646814054067562 1102 10-11 0.3935599102223293 1102 12-13 0.4215643316357358 1102 14-15 0.47448241803595437 1102 16-17 0.5604557331638915 1102 18-19 0.46109521808127596 1102 20-21 0.286548275924865 1102 22-23 0.22166211751854092 1102 24-25 0.34335540048984825 1102 26-27 0.3345795949689645 1102 28-29 0.3629622054343784 1102 30-31 0.34331071064465135 1102 32-33 0.3998096950496546 1102 34-35 0.42563646846738834 1102 36-37 0.37415139743374937 1102 38-39 0.5625078615362042 1102 40-41 0.5624192129361845 1102 42-43 0.6164472533132255 1102 44-45 0.5678913606925704 1102 46-47 0.6310732137251875 1102 48-49 0.8025971099986435 1102 50-51 0.88756744845778 1102 52-53 0.5481660827253236 1102 54-55 0.601005219799049 1102 56-57 0.7323267889278355 1102 58-59 0.7578095653405406 1102 60-61 0.6869860650586865 1102 62-63 0.7395686400374366 1102 64-65 0.5687581049698416 1102 66-67 0.7836630836636544 1102 68-69 0.987703537079561 1102 70-71 0.9549067984551236 1102 72-73 0.8960819074463728 1102 74-75 0.8906212745664028 1102 76-77 0.9466659021886521 1102 78-79 1.13441598418434 1102 80-81 1.3120149487210533 1102 82-83 0.9985108970620793 1102 84-85 1.0795963974719847 1102 86-87 1.2436669559288802 1102 88-89 1.2266364976696735 1102 90-91 1.1968809826116882 1102 92-93 1.025668700310689 1102 94-95 1.4427001980379863 1102 96-97 1.4679271501536455 1102 98-99 1.4876540109811778 1102 100-101 1.7179534870731636 1103 1 0.3713136408908113 1103 2 0.1711457069297495 1103 3 0.2933096108480626 1103 4 0.2024419166261211 1103 5 0.23235998153034387 1103 6 0.31738672480700814 1103 7 0.1577384665424688 1103 8 0.0811187959943922 1103 9 0.332506628842701 1103 10-11 0.20191393206939523 1103 12-13 0.25745890296973073 1103 14-15 0.49915553951890956 1103 16-17 0.5678125686488329 1103 18-19 0.5863310671378841 1103 20-21 0.5541831650344307 1103 22-23 0.3823332529041821 1103 24-25 0.34940885960934054 1103 26-27 0.34743129970249953 1103 28-29 0.3582369488968098 1103 30-31 0.43842408369993535 1103 32-33 0.4799776857812006 1103 34-35 0.5484476237139901 1103 36-37 0.8775567267948929 1103 38-39 0.6615644653097874 1103 40-41 0.9597834962860716 1103 42-43 0.7756701927372518 1103 44-45 1.099205495085151 1103 46-47 1.5247301154133694 1103 48-49 1.8656101851393174 1103 50-51 2.0131343137341773 1103 52-53 1.9722806143373717 1103 54-55 1.976377613046317 1103 56-57 1.933795673403182 1103 58-59 2.3165401181376204 1103 60-61 2.2571449528441896 1103 62-63 2.2578117714508785 1103 64-65 2.2925043810373715 1103 66-67 1.9344947619158894 1103 68-69 2.029270898880288 1103 70-71 1.814609711397857 1103 72-73 1.817527620786322 1103 74-75 1.850564174268488 1103 76-77 1.819712906491862 1103 78-79 1.8696816247007249 1103 80-81 2.057240039750514 1103 82-83 1.9652644554864445 1103 84-85 1.7378676652641047 1103 86-87 1.6287132981996493 1103 88-89 1.7080830385501784 1103 90-91 1.7845795923435617 1103 92-93 1.7465558237797403 1103 94-95 1.9057794764226266 1103 96-97 2.1141535652479853 1103 98-99 2.162360234086112 1103 100-101 2.220531275884813 1104 1 0.19691482476059008 1104 2 0.18725743463970446 1104 3 0.11441578916844719 1104 4 0.13102127289693044 1104 5 0.11907844249963517 1104 6 0.1962990444518482 1104 7 0.15479729007187615 1104 8 0.13561380154378355 1104 9 0.3155069988020074 1104 10-11 0.31111666976824637 1104 12-13 0.2938166536171636 1104 14-15 0.2522816956417344 1104 16-17 0.3001100159296328 1104 18-19 0.2363403161204971 1104 20-21 0.32457717169369715 1104 22-23 0.2835171226785036 1104 24-25 0.37350245931337156 1104 26-27 0.37299548764182333 1104 28-29 0.5666257761258109 1104 30-31 0.6328746941327807 1104 32-33 0.5097039158958907 1104 34-35 0.28999405360670494 1104 36-37 0.37369990104572537 1104 38-39 0.7306451164381684 1104 40-41 0.6711782428639452 1104 42-43 0.7463879137879417 1104 44-45 0.6711163348927798 1104 46-47 0.5659158349842173 1104 48-49 0.6943282761493066 1104 50-51 0.7319559933494233 1104 52-53 1.107325009453909 1104 54-55 1.0699680680962587 1104 56-57 0.958268481394299 1104 58-59 1.1687228780340355 1104 60-61 0.8806651156262824 1104 62-63 1.0977858742995643 1104 64-65 1.0402106333496128 1104 66-67 1.1010134448607651 1104 68-69 1.1110781500826583 1104 70-71 1.273830947061935 1104 72-73 1.0791443429468863 1104 74-75 0.9493803044941593 1104 76-77 1.444111722622086 1104 78-79 1.5385310512638029 1104 80-81 1.7337383749336475 1104 82-83 1.4578375224490792 1104 84-85 1.1597692560891133 1104 86-87 1.3485431169195934 1104 88-89 1.1045776741402662 1104 90-91 1.3498866585662768 1104 92-93 1.611659412384995 1104 94-95 1.6825737790493385 1104 96-97 1.875280091330115 1104 98-99 2.142955868566318 1104 100-101 1.9931727853187766 1105 1 0.2182436440580915 1105 2 0.13014077502622 1105 3 0.23345937782693227 1105 4 0.17121029814731514 1105 5 0.06612315966707882 1105 6 0.08653744196912783 1105 7 0.2302804329693231 1105 8 0.09641222163124041 1105 9 0.08368304619938272 1105 10-11 0.22758584736734377 1105 12-13 0.21010244851066062 1105 14-15 0.2876040260127368 1105 16-17 0.21509097234097396 1105 18-19 0.38522591352492697 1105 20-21 0.4596455875492538 1105 22-23 0.2200102810929465 1105 24-25 0.451898565957471 1105 26-27 0.5983023010146624 1105 28-29 0.2915940368113823 1105 30-31 0.38448987257826417 1105 32-33 0.42297800250894824 1105 34-35 0.5042414791956489 1105 36-37 0.10471273783511492 1105 38-39 0.05861097904220003 1105 40-41 0.38888172713534885 1105 42-43 0.5135553563045221 1105 44-45 0.4292980248349423 1105 46-47 0.3308192064047333 1105 48-49 0.5636046197802926 1105 50-51 0.42961433726252807 1105 52-53 0.3427672439016476 1105 54-55 0.3014816807355629 1105 56-57 0.23282060440178043 1105 58-59 0.34433297818920394 1105 60-61 0.16388559806386027 1105 62-63 0.051961357442461775 1105 64-65 -0.11219450136614384 1105 66-67 0.2367095938239494 1105 68-69 0.018575681469311434 1105 70-71 -0.10869127045816995 1105 72-73 -0.2255812883128101 1105 74-75 -0.11720877718167344 1105 76-77 -0.1345312453564702 1105 78-79 0.20203083966837454 1105 80-81 -0.08624328679579563 1105 82-83 0.16193542060114297 1105 84-85 0.10599580426233857 1105 86-87 -0.22179120385908035 1105 88-89 0.05943026265768481 1105 90-91 0.3002745835204337 1105 92-93 0.42757138868630307 1105 94-95 0.3623029368995283 1105 96-97 0.21522930842702692 1105 98-99 0.21526508002068212 1105 100-101 0.5320217061820856 1106 1 -0.005429591266690181 1106 2 -0.042058422137792206 1106 3 -0.11971677690146265 1106 4 0.04794369403929721 1106 5 -0.11066367173736325 1106 6 0.09515880574767266 1106 7 0.10157904625260983 1106 8 0.15363383564437783 1106 9 0.1774102383586964 1106 10-11 0.005287599009513144 1106 12-13 -0.04230866607593242 1106 14-15 -0.03228245610592495 1106 16-17 -0.13194987324342833 1106 18-19 -0.02749173844045316 1106 20-21 0.05089324159992259 1106 22-23 0.20341268960092407 1106 24-25 0.36455105266375654 1106 26-27 0.07770030435111863 1106 28-29 0.41397765165863376 1106 30-31 0.3240458365027692 1106 32-33 0.21281026713203488 1106 34-35 0.4334882991310067 1106 36-37 0.6333333196305517 1106 38-39 0.5407687311014229 1106 40-41 0.19521383536181958 1106 42-43 0.15905308855349887 1106 44-45 0.15316023941110046 1106 46-47 0.18943991853068098 1106 48-49 0.13012857452953597 1106 50-51 -0.44598518312116653 1106 52-53 -0.30187682345316347 1106 54-55 -0.38762464290118004 1106 56-57 -0.1562029593559089 1106 58-59 0.06531814657623158 1106 60-61 0.12504384537905366 1106 62-63 0.26310982996878707 1106 64-65 0.02463625142292969 1106 66-67 0.10076834682154967 1106 68-69 0.02244323959672201 1106 70-71 0.026660230949403285 1106 72-73 0.13642801728618537 1106 74-75 0.08292676655724662 1106 76-77 -0.06939531062599258 1106 78-79 0.05505385603788682 1106 80-81 0.4041774201169339 1106 82-83 0.2934193638813021 1106 84-85 0.20365246154520733 1106 86-87 0.2144478483773895 1106 88-89 0.20788649766967637 1106 90-91 0.14832549062084155 1106 92-93 0.22626938680954467 1106 94-95 0.677868962340046 1106 96-97 0.8239054110232118 1106 98-99 0.8099795258553222 1106 100-101 0.9713430751738485 1107 1 -0.03519502190287227 1107 2 -0.08809117892015905 1107 3 -0.07035206892847157 1107 4 0.15702437168413041 1107 5 0.07680671091871716 1107 6 0.10080831409976554 1107 7 -0.13540413109604543 1107 8 -0.391110566677618 1107 9 -0.3219455844111181 1107 10-11 -0.1623539718944116 1107 12-13 -0.3315960727813021 1107 14-15 -0.2587403698602131 1107 16-17 -0.20486407179809873 1107 18-19 -0.18675900535196632 1107 20-21 -0.013831761361807082 1107 22-23 -0.32296895949278337 1107 24-25 -0.31703288288890263 1107 26-27 -0.4975980903888413 1107 28-29 -0.29449513000231065 1107 30-31 -0.5272417775239973 1107 32-33 -0.4449576217436899 1107 34-35 -0.2243832504484331 1107 36-37 -0.20323817663766874 1107 38-39 0.07511004137271726 1107 40-41 0.3039131897037137 1107 42-43 -0.13274068588080468 1107 44-45 0.010767454196077608 1107 46-47 0.1017762827055364 1107 48-49 -0.07861076322665639 1107 50-51 -0.39046747194950626 1107 52-53 -0.24261622044404163 1107 54-55 -0.13323757383353296 1107 56-57 -0.38824004027049597 1107 58-59 -0.4449331565868988 1107 60-61 -0.43229903461147146 1107 62-63 -0.0726768066856387 1107 64-65 -0.2883783499276227 1107 66-67 -0.6081894188460275 1107 68-69 -0.3050542429290424 1107 70-71 -0.274766584593781 1107 72-73 -0.18610115579756936 1107 74-75 -0.3376395170584381 1107 76-77 -0.025594961141333528 1107 78-79 -0.2096844747286113 1107 80-81 -0.022475227673609766 1107 82-83 -0.03337100455845743 1107 84-85 -0.3190788499107775 1107 86-87 -0.36777360050307095 1107 88-89 -0.6247940200414952 1107 90-91 -0.45810073257546335 1107 92-93 -0.5839648863808335 1107 94-95 -0.6432904085875712 1107 96-97 -0.734048326685592 1107 98-99 -0.1448947485197536 1107 100-101 0.2711927671534724 1108 1 0.12146852683843434 1108 2 -0.10923071285809982 1108 3 0.03209211342949203 1108 4 -0.08317246429944447 1108 5 0.057855147211370195 1108 6 0.08746088232132365 1108 7 0.14604692425390908 1108 8 0.1793070997585886 1108 9 0.2230867471192468 1108 10-11 0.24765456850983725 1108 12-13 0.29034869927124163 1108 14-15 0.277771891720171 1108 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-0.393184376559347 2316 80-81 -0.4756365173782875 2316 82-83 -0.2973625189031388 2316 84-85 -0.6499140548451017 2316 86-87 -0.6006143316485861 2316 88-89 -0.4294707079542235 2316 90-91 -0.526994005826019 2316 92-93 -0.3254852169957587 2316 94-95 -0.183355439738353 2316 96-97 -0.1917828674210469 2316 98-99 -0.681741743343121 2316 100-101 -0.771209171330316 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 12.0 5 21.0 6 41.0 7 71.0 8 208.0 9 410.0 10 806.0 11 1477.0 12 2317.0 13 3329.0 14 4201.0 15 6261.0 16 8761.0 17 12983.0 18 11053.0 19 5055.0 20 3333.0 21 3011.0 22 3043.0 23 3151.0 24 3541.0 25 4004.0 26 4603.0 27 5363.0 28 6502.0 29 7961.0 30 9841.0 31 11915.0 32 15073.0 33 19365.0 34 27069.0 35 36930.0 36 53446.0 37 86257.0 38 72576.0 39 5584.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.476064453147224 20.979813449053477 16.83896498130622 18.70515711649308 2 15.356037010831605 22.652185090559144 41.24268849520074 20.74908940340851 3 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17.444419799006976 22-23 19.40180856509128 25.106978331342773 37.629642268099865 17.861570835466075 24-25 18.53238372469995 25.838386802851552 37.99981572041374 17.629413752034754 26-27 18.481865078687985 25.637551828279896 38.36674799938574 17.513835093646378 28-29 17.961321322414452 26.195641169437906 38.30647408851664 17.536563419631005 30-31 18.7534418649105 25.988867700401876 37.95677245233728 17.300917982350345 32-33 18.05259182754212 26.199229253777524 37.64633486056955 18.101844058110807 34-35 18.578507094610735 26.164291938056095 37.502132819484984 17.755068147848185 36-37 18.99433904737635 25.874626281564133 37.40267393549973 17.728360735559793 38-39 18.37851119521409 27.009115200988305 37.06268250593517 17.54969109786243 40-41 18.477987607400067 26.816544246041 36.85825864291134 17.847209503647594 42-43 18.367734633169714 26.990336543110168 36.1048135277021 18.53711529601802 44-45 18.926334205039364 26.828449845811548 35.69118628991069 18.554029659238395 46-47 19.05890730235791 27.295373705960575 34.464872538324954 19.18084645335657 48-49 19.187492179768633 28.17854014764597 33.32123804215531 19.312729630430084 50-51 19.462672713560806 28.512197868572187 32.36339604312905 19.66173337473795 52-53 19.49911103982746 29.130479788242063 31.62298078640272 19.74742838552775 54-55 19.383730942785416 28.17936844308401 31.91431519151342 20.52258542261716 56-57 20.01560617831765 28.613108962291516 31.013753229011563 20.35753163037927 58-59 19.863820842281513 28.61946274524078 31.01784024314782 20.49887616932989 60-61 20.243985667974748 28.45521242108855 31.60939543877609 19.69140647216061 62-63 20.079167377580617 28.393903201956434 31.49803787749531 20.02889154296764 64-65 19.771691835167648 28.251059352159942 32.006597844325 19.97065096834741 66-67 19.98719199943581 28.569689855290072 31.131744959266918 20.311373186007202 68-69 19.949336679038648 28.30537220023728 31.222209962724808 20.523081157999265 70-71 19.80832669775907 28.6941189853259 31.475941303605957 20.021613013309068 72-73 19.802621100771315 28.383540761302363 31.539441650929444 20.27439648699688 74-75 19.477527898167423 29.04389766690555 30.93847046377504 20.54010397115199 76-77 18.880520700743812 28.846816657054813 31.29562074931494 20.977041892886433 78-79 18.802969596447042 28.854025177409042 31.043112843299863 21.299892382844053 80-81 19.097943802344773 28.632640502669066 30.777560521733 21.491855173253168 82-83 18.723637571494223 29.08114281554149 30.804739435690465 21.390480177273822 84-85 18.594905953973413 28.321298899838936 30.469411154486636 22.61438399170102 86-87 18.164363305465507 28.330660296877664 30.567252459762273 22.937723937894557 88-89 18.35961671459251 27.74019036872566 30.811501292177773 23.088691624504058 90-91 17.888691474187333 27.869331689306165 31.321333754956537 22.920643081549965 92-93 17.871373339445302 27.885944248358356 31.389332183736773 22.85335022845957 94-95 17.572507865587742 28.14811191770195 31.19299866006329 23.08638155664702 96-97 17.917428771623307 28.598727853457458 30.144094710309115 23.33974866461012 98-99 17.13312365991958 28.66728469137628 29.903599519988173 24.29599212871597 100-101 16.99391904342663 27.981272564716804 30.673113891106972 24.3516945007496 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1197.0 1 815.0 2 436.0 3 424.0 4 360.0 5 372.5 6 556.5 7 1006.5 8 1613.0 9 1838.5 10 1607.0 11 1327.5 12 1285.5 13 1351.0 14 1402.0 15 1521.0 16 1637.5 17 1723.0 18 1822.0 19 1943.0 20 2095.5 21 2377.5 22 2670.5 23 2974.0 24 3327.5 25 3886.5 26 5064.0 27 6386.5 28 6874.5 29 7452.5 30 8418.0 31 9276.5 32 10754.5 33 12001.0 34 14030.0 35 16150.5 36 17671.5 37 18870.5 38 17744.5 39 15834.5 40 14844.5 41 14412.0 42 13653.5 43 13732.0 44 14574.5 45 14323.0 46 13554.5 47 13245.0 48 14629.0 49 14247.0 50 11941.0 51 10584.0 52 9261.0 53 8418.0 54 7565.5 55 6660.5 56 6130.0 57 5518.0 58 4670.5 59 3980.0 60 3645.0 61 3296.5 62 2667.5 63 1852.0 64 1164.5 65 818.0 66 626.0 67 451.5 68 323.0 69 251.5 70 205.0 71 179.5 72 133.5 73 132.5 74 125.0 75 103.0 76 81.0 77 35.5 78 13.0 79 8.0 80 5.5 81 4.0 82 3.5 83 3.0 84 2.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02820906557470284 2 0.00636978900073935 3 0.008872206108172668 4 2.2749246431211966E-4 5 0.005004834214866633 6 0.0 7 0.0 8 2.2749246431211966E-4 9 2.2749246431211966E-4 10-11 0.0 12-13 2.2749246431211966E-4 14-15 0.0 16-17 0.0 18-19 0.004891087982710572 20-21 0.0017061934823408974 22-23 0.0 24-25 0.005801057839959051 26-27 0.0051185804470226925 28-29 0.00693852016151965 30-31 0.030256497753511916 32-33 2.2749246431211966E-4 34-35 0.0036398794289939146 36-37 0.016606949894784735 38-39 0.007848490018768129 40-41 0.008758459876016608 42-43 0.00739350509014389 44-45 0.003526133196837855 46-47 0.002616163339589376 48-49 0.0022749246431211967 50-51 0.0023886708752772563 52-53 0.003753625661149974 54-55 0.0023886708752772563 56-57 0.0012512085537166582 58-59 0.0015924472501848376 60-61 0.0 62-63 0.0 64-65 0.008530967411704489 66-67 0.0013649547858727178 68-69 0.0014787010180287777 70-71 0.0056873116078029915 72-73 0.01478701018028778 74-75 0.00682477392936359 76-77 0.0030711482682136155 78-79 0.012284593072854462 80-81 3.4123869646817946E-4 82-83 0.00659728146505147 84-85 6.824773929363589E-4 86-87 0.0 88-89 0.0015924472501848376 90-91 9.099698572484786E-4 92-93 1.1374623215605983E-4 94-95 4.549849286242393E-4 96-97 6.824773929363589E-4 98-99 5.687311607802992E-4 100-101 0.0013649547858727178 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 439575.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.416498703997924 #Duplication Level Percentage of deduplicated Percentage of total 1 83.0338528688971 41.86276135552705 2 9.167204664663519 9.243567241905842 3 2.724021765185718 4.1200691938244365 4 1.257928029558687 2.5368130748787285 5 0.7013155187981359 1.7678936472289926 6 0.45628816664190264 1.380267105729064 7 0.3483042672965789 1.229219714852844 8 0.253446784358061 1.02223195800965 9 0.22864687049424143 1.0374817184951493 >10 1.5733645214475769 15.81216669765346 >50 0.15891525742538634 5.4536205007888094 >100 0.08990063134341214 8.409572546059481 >500 0.004086392333791461 1.3666099055441407 >1k 0.0018161743705739828 1.7775085287353858 >5k 9.080871852869914E-4 2.98021681076703 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7391 1.6813968037308766 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5628 1.2803275891486094 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3729 0.8483193994198942 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1479 0.336461354717625 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1328 0.3021099926064949 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1229 0.2795882386395951 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 918 0.20883808223852585 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 881 0.2004208610589774 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 727 0.165387021554911 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 602 0.13695046351589601 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 599 0.13626798612295968 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 572 0.13012568958653245 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 561 0.12762327247909913 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 559 0.12716828755047488 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 551 0.12534834783597795 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 482 0.10965136779844167 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 482 0.10965136779844167 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 462 0.10510151851219927 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 458 0.1041915486549508 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 455 0.10350907126201446 No Hit TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT 450 0.10237160894045386 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 449 0.10214411647614173 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 447 0.10168913154751749 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.1374623215605983E-4 0.0 16-17 0.0 0.0 0.0 6.82477392936359E-4 0.0 18-19 0.0 0.0 0.0 6.82477392936359E-4 0.0 20-21 0.0 0.0 0.0 0.0010237160894045385 0.0 22-23 0.0 0.0 0.0 0.0018199397144969573 0.0 24-25 0.0 0.0 0.0 0.002729909571745436 0.0 26-27 0.0 0.0 0.0 0.004208610589774214 0.0 28-29 0.0 0.0 0.0 0.008872206108172666 0.0 30-31 0.0 0.0 0.0 0.0138770403230393 0.0 32-33 0.0 0.0 0.0 0.021839276573963486 0.0 34-35 0.0 0.0 0.0 0.02820906557470284 0.0 36-37 0.0 0.0 0.0 0.03571631689700279 0.0 38-39 0.0 0.0 0.0 0.047204686344764824 0.0 40-41 0.0 0.0 0.0 0.07211511118694193 0.0 42-43 0.0 0.0 0.0 0.11931979753170675 0.0 44-45 0.0 0.0 0.0 0.1602684411078883 0.0 46-47 0.0 0.0 0.0 0.2572939771370073 0.0 48-49 0.0 0.0 0.0 0.3503383950406643 0.0 50-51 0.0 0.0 0.0 0.4134675538872775 0.0 52-53 0.0 0.0 0.0 0.492407439003583 0.0 54-55 0.0 0.0 0.0 0.5478018540635842 0.0 56-57 0.0 0.0 0.0 0.7227435591196041 0.0 58-59 0.0 0.0 0.0 0.9434112495023603 0.0 60-61 0.0 0.0 0.0 1.155547972473412 0.0 62-63 0.0 0.0 0.0 1.4305863618267645 0.0 64-65 0.0 0.0 0.0 1.7893419780469773 0.0 66-67 0.0 0.0 0.0 2.2343172382414833 0.0 68-69 0.0 0.0 0.0 2.7349144059603026 0.0 70-71 0.0 0.0 0.0 3.066257180230905 0.0 72-73 0.0 0.0 0.0 3.3383381675482 0.0 74-75 0.0 0.0 0.0 3.696638798839788 0.0 76-77 0.0 0.0 0.0 4.155036114428709 0.0 78-79 0.0 0.0 0.0 4.534607291133481 0.0 80-81 0.0 0.0 0.0 4.925666837286014 0.0 82-83 0.0 0.0 0.0 5.467440141045328 0.0 84-85 0.0 0.0 0.0 6.4834214866632545 0.0 86-87 0.0 0.0 0.0 7.654211454245578 0.0 88-89 0.0 0.0 0.0 8.702155491099358 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3395 0.0 45.493183 1 GTATCAA 5710 0.0 38.034946 1 GGATATG 125 5.6097633E-9 34.216476 1 GTCTTAC 85 1.6245544E-5 33.545563 1 TATCAAC 7320 0.0 29.78562 2 ATCAACG 7385 0.0 29.459137 3 TCAACGC 7415 0.0 29.33995 4 CAACGCA 7495 0.0 29.026781 5 AACGCAG 7665 0.0 28.376547 6 TAAGGGT 135 4.2141255E-7 28.148891 3 TAGGGAC 70 0.008384954 27.143572 3 CATTCCG 210 4.3655746E-11 27.137396 9 TCGCGTC 35 0.008398755 27.137394 58-59 CTAGATC 145 7.8671656E-7 26.207586 4 CCGGGTC 145 7.882809E-7 26.201624 9 TAGGACA 205 9.531504E-10 25.488476 4 TACTAGA 170 1.1143311E-7 25.159172 1 ACGCAGA 8630 0.0 25.148474 7 GGACAAT 170 1.1217162E-7 25.142 6 CGCAGAG 8645 0.0 25.049906 8 >>END_MODULE