FastQCFastQC Report
Thu 26 May 2016
SRR936881_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936881_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464343
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64341.3856136519770945No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45280.975141221037035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30040.6469355627197998No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13040.2808268887438811No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9630.20738979590518217No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8450.1819775467703831No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC7340.15807280394019077No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7020.15118134654770288No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6680.1438591730681845No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA6140.13222983871836122No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA5380.1158626274112025No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT5240.11284761480198904No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC5070.10918652806222987No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA5010.10789437980113839No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT4650.10014149023458953No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG1550.045.975359
GGTATCA37900.037.4717451
ATTACTC651.325673E-436.544513
CGTCACT651.3273547E-436.5366361
GTATCAA61500.034.986061
TATCAAC74250.028.9845962
ATCAACG75100.028.6565423
TCAACGC75150.028.640564
CAACGCA75700.028.4952375
AACGCAG76000.028.3827556
ATGGGGG10150.028.0864945
CTTTAGG2550.027.9458032
ACTGTCC1056.884293E-527.1502768
TACGTGA700.00837533327.1502765
GCATTCC2650.026.894148
CATGGGG20050.026.5409184
ACGTGAT908.925226E-426.39616
ACGCAGA83550.025.704217
GTACATG44050.025.6627371
CGCAGAG83650.025.6166868
Produced by FastQC (version 0.11.5)