##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936881_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 464343 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.94535935719931 33.0 31.0 34.0 30.0 34.0 2 32.03411486767325 33.0 31.0 34.0 30.0 34.0 3 32.0035964793267 33.0 31.0 34.0 30.0 34.0 4 35.601686684196814 37.0 35.0 37.0 33.0 37.0 5 35.61708047714728 37.0 35.0 37.0 33.0 37.0 6 35.760409869428415 37.0 35.0 37.0 35.0 37.0 7 35.72076030003683 37.0 35.0 37.0 33.0 37.0 8 35.691303626844814 37.0 35.0 37.0 33.0 37.0 9 37.29426092349836 39.0 37.0 39.0 34.0 39.0 10-11 37.19065539913383 39.0 37.0 39.0 34.0 39.0 12-13 37.09663115412529 39.0 37.0 39.0 33.0 39.0 14-15 38.18349366739673 40.0 38.0 41.0 33.0 41.0 16-17 38.14581570089352 40.0 38.0 41.0 33.0 41.0 18-19 38.21991501971603 40.0 38.0 41.0 33.5 41.0 20-21 38.18513146531767 40.0 38.0 41.0 33.5 41.0 22-23 38.05225662925898 40.0 38.0 41.0 33.0 41.0 24-25 38.10308543468945 40.0 38.0 41.0 33.5 41.0 26-27 38.01379368268715 40.0 38.0 41.0 33.0 41.0 28-29 37.90025369177526 40.0 37.0 41.0 33.0 41.0 30-31 37.60187189211423 40.0 37.0 41.0 33.0 41.0 32-33 37.24743131693597 39.0 35.5 41.0 32.5 41.0 34-35 37.00587819779775 39.0 35.0 41.0 32.0 41.0 36-37 36.762362090092886 39.0 35.0 41.0 31.0 41.0 38-39 36.55325912095154 39.0 35.0 41.0 31.0 41.0 40-41 36.31002405549346 39.0 35.0 41.0 30.5 41.0 42-43 35.94451300008829 39.0 35.0 41.0 30.0 41.0 44-45 35.4289458439128 39.0 35.0 40.5 28.0 41.0 46-47 34.71308386257573 38.0 35.0 40.0 23.5 41.0 48-49 34.139064226229316 38.5 34.0 40.0 17.0 41.0 50-51 33.28922477565075 38.0 33.5 39.5 10.5 40.5 52-53 33.42424780819351 38.0 33.5 39.5 8.5 40.5 54-55 33.77630975378115 39.0 34.0 40.5 6.0 41.0 56-57 33.68733673168326 38.5 34.0 41.0 2.0 41.0 58-59 33.44823115670959 38.0 34.0 40.5 2.0 41.0 60-61 33.18934386864882 38.0 34.0 40.0 2.0 41.0 62-63 32.847446822715106 38.0 33.0 40.0 2.0 41.0 64-65 32.536211378226874 37.0 33.0 40.0 2.0 41.0 66-67 32.17928772480688 36.5 33.0 39.5 2.0 41.0 68-69 31.768986503511414 36.0 32.0 39.0 2.0 41.0 70-71 31.385525355179254 36.0 32.0 39.0 2.0 40.5 72-73 30.983059936297092 35.0 31.0 38.5 2.0 40.0 74-75 30.537446456606432 35.0 31.0 37.5 2.0 39.5 76-77 30.123169725827672 35.0 31.0 37.0 2.0 39.0 78-79 29.694357403901854 35.0 30.0 36.5 2.0 39.0 80-81 29.262538683688568 35.0 30.0 36.0 2.0 38.5 82-83 28.86197164595999 34.5 29.0 35.5 2.0 37.0 84-85 28.473821076230287 34.0 29.0 35.0 2.0 37.0 86-87 28.12700202221203 34.0 29.0 35.0 2.0 36.0 88-89 27.7680055476232 34.0 28.0 35.0 2.0 36.0 90-91 27.376331720301586 34.0 27.0 35.0 2.0 36.0 92-93 27.0508492644446 34.0 26.0 35.0 2.0 35.0 94-95 26.68214229567367 34.0 25.0 35.0 2.0 35.0 96-97 26.21979980316275 34.0 24.5 35.0 2.0 35.0 98-99 25.69822954152426 34.0 21.0 35.0 2.0 35.0 100-101 23.57808882657863 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5057748580367907 1101 2 0.5315970578255111 1101 3 0.4465942442695088 1101 4 0.4353714369345738 1101 5 0.4349177690926638 1101 6 0.31401405630215606 1101 7 0.3534627256402132 1101 8 0.3888073731199455 1101 9 0.5579222826037054 1101 10-11 0.4744821750456296 1101 12-13 0.387903051587827 1101 14-15 0.5708259734111962 1101 16-17 0.6726095722899998 1101 18-19 0.6735841849578463 1101 20-21 0.6357413205187754 1101 22-23 0.6683359519728853 1101 24-25 0.7832719498310325 1101 26-27 0.5391677437867841 1101 28-29 0.43576433509581136 1101 30-31 0.6156902229529777 1101 32-33 0.7211505928584216 1101 34-35 0.650370724304203 1101 36-37 0.544439385738734 1101 38-39 0.6127301362312281 1101 40-41 0.8063016746097063 1101 42-43 0.7427022444310012 1101 44-45 0.9033370895200363 1101 46-47 0.6978949556468876 1101 48-49 1.301985591215292 1101 50-51 1.4998818804712215 1101 52-53 1.5253250872900495 1101 54-55 1.5818048861047984 1101 56-57 1.5875350616330408 1101 58-59 1.3811851639768236 1101 60-61 1.409907650977864 1101 62-63 1.5489897603748872 1101 64-65 1.5524970992878266 1101 66-67 1.4852087547659742 1101 68-69 1.4744477329245207 1101 70-71 1.5705406749595063 1101 72-73 1.7114356334029885 1101 74-75 1.7127561781621345 1101 76-77 1.9978649630308176 1101 78-79 1.6710328474774734 1101 80-81 1.6719520484890502 1101 82-83 1.376893744135284 1101 84-85 1.4592958319142433 1101 86-87 1.565488010734267 1101 88-89 1.537642908126486 1101 90-91 1.4975444713200083 1101 92-93 1.4323504065286876 1101 94-95 1.75620291640708 1101 96-97 1.7733059627220307 1101 98-99 1.5069010343708094 1101 100-101 1.5817024278382412 1102 1 0.3409655003387009 1102 2 0.48920921247394844 1102 3 0.2953556677006368 1102 4 0.35811588560810037 1102 5 0.17084963462872338 1102 6 0.20692122878468666 1102 7 0.1833521895973078 1102 8 0.17406256344833793 1102 9 0.2540913866614076 1102 10-11 0.30328766378601557 1102 12-13 0.25106614031879815 1102 14-15 0.3959174981964608 1102 16-17 0.47974536642691845 1102 18-19 0.46405030411076353 1102 20-21 0.572165438329705 1102 22-23 0.349122417683958 1102 24-25 0.12369804528363915 1102 26-27 0.2563149564499625 1102 28-29 0.28948752019758217 1102 30-31 0.2809527901986826 1102 32-33 0.44388248507695494 1102 34-35 0.5658687250811312 1102 36-37 0.572819916955261 1102 38-39 0.5091138771915595 1102 40-41 0.3788485553116132 1102 42-43 0.5354736502438584 1102 44-45 0.3044559873771959 1102 46-47 0.6423424520074761 1102 48-49 0.567639959095878 1102 50-51 0.690840055704399 1102 52-53 0.889275897377594 1102 54-55 0.8094759776138858 1102 56-57 0.8555928134782604 1102 58-59 0.8175965291381573 1102 60-61 0.7258859361584982 1102 62-63 0.8085591524462714 1102 64-65 0.9745018942840815 1102 66-67 1.1719389361675105 1102 68-69 0.9622964197939972 1102 70-71 0.852966739020971 1102 72-73 0.5760662578119486 1102 74-75 0.7303842638128515 1102 76-77 1.0194483320061707 1102 78-79 0.6756355730129862 1102 80-81 0.4863558719295966 1102 82-83 0.6646523776790687 1102 84-85 0.6986748367367106 1102 86-87 0.6290423115162973 1102 88-89 0.6564475728495189 1102 90-91 0.7825740378476347 1102 92-93 0.8872894096246391 1102 94-95 0.7940204083336759 1102 96-97 0.7944596655028917 1102 98-99 0.7377504785514795 1102 100-101 0.9182487748908095 1103 1 0.09270073411475366 1103 2 0.28049093151077287 1103 3 0.26192947182546433 1103 4 0.2795347388636884 1103 5 0.3691802684695631 1103 6 0.27968874849098313 1103 7 0.22095288066738306 1103 8 0.20244178815408986 1103 9 0.2974387573557138 1103 10-11 0.17037869067296185 1103 12-13 0.35693051781582597 1103 14-15 0.4720831390559823 1103 16-17 0.5070415547435161 1103 18-19 0.5020094877842283 1103 20-21 0.429030787644038 1103 22-23 0.49555692799602014 1103 24-25 0.2739723137211669 1103 26-27 0.25155197189106104 1103 28-29 0.16522448164882064 1103 30-31 0.365922015984232 1103 32-33 0.2730283656514132 1103 34-35 0.20965989234168347 1103 36-37 0.25433919133167393 1103 38-39 0.16486811539478197 1103 40-41 -0.051601720036615006 1103 42-43 -0.01256219901648592 1103 44-45 0.0995396716445569 1103 46-47 0.2026145608510177 1103 48-49 0.4048119707576845 1103 50-51 0.07275561557914756 1103 52-53 0.3723576380103495 1103 54-55 0.5883130362468592 1103 56-57 0.5848283172035522 1103 58-59 0.4602031590917264 1103 60-61 0.21522941958361486 1103 62-63 0.16890900667367958 1103 64-65 0.38076687646743324 1103 66-67 0.5387913279178598 1103 68-69 0.49952026171171937 1103 70-71 0.5846566213230986 1103 72-73 0.47531579889822595 1103 74-75 0.6008345391610881 1103 76-77 0.3232924093196381 1103 78-79 0.4538452695900297 1103 80-81 0.577860029148038 1103 82-83 0.4593073825381566 1103 84-85 0.4199079649086137 1103 86-87 0.6167077904062666 1103 88-89 0.40727253775611416 1103 90-91 0.6043816180225647 1103 92-93 0.5612911372998362 1103 94-95 0.6373623800429904 1103 96-97 0.8309999959207737 1103 98-99 0.7612393026525517 1103 100-101 0.8648268982036278 1104 1 0.2995430403385022 1104 2 0.2784901678605216 1104 3 0.32635864326494257 1104 4 0.3102563883482219 1104 5 0.35544983700717125 1104 6 0.398780004695638 1104 7 0.2773179054860151 1104 8 0.27125431201816497 1104 9 0.16770985319381992 1104 10-11 0.47726299766036817 1104 12-13 0.4520469744786766 1104 14-15 0.6693989084336138 1104 16-17 0.6258235325976571 1104 18-19 0.4908754671656723 1104 20-21 0.5572400278120426 1104 22-23 0.5322999596874993 1104 24-25 0.5542281365543076 1104 26-27 0.4154274969006053 1104 28-29 0.4769656042146906 1104 30-31 0.3118555784126471 1104 32-33 0.6713177890954682 1104 34-35 0.6411795563355795 1104 36-37 0.4540528224885989 1104 38-39 0.6196180199384997 1104 40-41 0.4930145457136703 1104 42-43 0.6365176024344521 1104 44-45 0.9796160366693769 1104 46-47 1.3475286501980932 1104 48-49 1.6391762319260792 1104 50-51 1.4076429771675407 1104 52-53 1.317554277949256 1104 54-55 1.2654837120773337 1104 56-57 1.381964628772856 1104 58-59 1.5739985008252049 1104 60-61 1.4971156356966375 1104 62-63 1.5694244705912013 1104 64-65 1.597479362148988 1104 66-67 1.4552938097811712 1104 68-69 1.3802304564425292 1104 70-71 1.2434118714185551 1104 72-73 1.422413927955116 1104 74-75 1.3189674143042716 1104 76-77 1.1839835127942493 1104 78-79 1.4531770756228646 1104 80-81 1.4422433815726272 1104 82-83 1.4127438086549944 1104 84-85 1.5616185414645507 1104 86-87 1.5675172596693443 1104 88-89 1.3039659321814696 1104 90-91 1.3292613431084739 1104 92-93 1.4727191632639425 1104 94-95 1.5459114445714377 1104 96-97 1.5495566969517007 1104 98-99 1.288971261415437 1104 100-101 1.2633415984709053 1105 1 0.23358374656352865 1105 2 0.153630960892972 1105 3 0.18025313996123415 1105 4 0.024893124389087973 1105 5 0.36116047414496677 1105 6 0.17853665694222798 1105 7 -0.028498909327204558 1105 8 0.04942547330061586 1105 9 0.19140612764876153 1105 10-11 0.06335479830460855 1105 12-13 0.13451653807408093 1105 14-15 0.07825649403935842 1105 16-17 0.09247262255454558 1105 18-19 -0.024211806579032213 1105 20-21 0.19002668960027336 1105 22-23 -0.020575446549720766 1105 24-25 -0.027645253741916065 1105 26-27 -0.14556547958964927 1105 28-29 -0.18729233579492188 1105 30-31 -0.34073537209278015 1105 32-33 -0.17266559553316085 1105 34-35 -0.6894509094831065 1105 36-37 -0.3592771389861156 1105 38-39 -0.567701280723675 1105 40-41 -0.3532950626485416 1105 42-43 -0.20400424649193383 1105 44-45 -0.3503992443068853 1105 46-47 -0.234100818411342 1105 48-49 -0.3596974238143318 1105 50-51 -0.010307865438406338 1105 52-53 0.1460791256775522 1105 54-55 0.1800411735699896 1105 56-57 0.4149837335802644 1105 58-59 0.4146189945513967 1105 60-61 0.2532190584918368 1105 62-63 0.48143510270707424 1105 64-65 0.5130053362215463 1105 66-67 0.5848162254667706 1105 68-69 0.8611579327857122 1105 70-71 0.8968780703295174 1105 72-73 0.76797024875518 1105 74-75 0.7109621197688334 1105 76-77 0.4677523180801728 1105 78-79 0.6319555301637543 1105 80-81 0.7311686195756266 1105 82-83 0.7128912377921566 1105 84-85 0.8200069365315947 1105 86-87 0.7542195819472859 1105 88-89 0.7756387327611414 1105 90-91 0.6312292171874923 1105 92-93 0.5967013282841371 1105 94-95 0.4746916926541438 1105 96-97 0.7197960989131182 1105 98-99 0.5519421558188675 1105 100-101 0.6875841857625851 1106 1 -0.2390544993796091 1106 2 -0.0941628527082301 1106 3 0.010979391310161901 1106 4 0.26929319296996823 1106 5 0.3826424262795456 1106 6 0.2967579916471834 1106 7 0.15183854894435456 1106 8 0.24508268992543236 1106 9 0.1914162130723014 1106 10-11 0.27274584043141914 1106 12-13 0.23090797353241044 1106 14-15 0.5138383725503459 1106 16-17 0.23700934862435474 1106 18-19 0.03350707232528549 1106 20-21 0.21610003080023432 1106 22-23 0.06934114699762972 1106 24-25 0.05844896428477142 1106 26-27 -0.20624190894630345 1106 28-29 -0.06617648453475056 1106 30-31 -0.046155278701753844 1106 32-33 -0.11613427525036002 1106 34-35 -0.2128843910721656 1106 36-37 -0.16764148499682818 1106 38-39 -0.33728969458911706 1106 40-41 -0.30136017414289284 1106 42-43 -0.39346397035303937 1106 44-45 0.11448331093602349 1106 46-47 0.46874982655150177 1106 48-49 0.15795206737604417 1106 50-51 0.16112920656142649 1106 52-53 0.2539679439684832 1106 54-55 0.2841101376961319 1106 56-57 0.6879684138219133 1106 58-59 0.5496073458872175 1106 60-61 0.5575643632229088 1106 62-63 0.8010346105384158 1106 64-65 0.6994142194626036 1106 66-67 0.659773614552325 1106 68-69 1.0018874114701752 1106 70-71 0.771886895075113 1106 72-73 0.6271676507500779 1106 74-75 0.9419939594509437 1106 76-77 0.7948705091586064 1106 78-79 0.6535232083984042 1106 80-81 0.6891804800337091 1106 82-83 0.7148629380937095 1106 84-85 0.6642879971147302 1106 86-87 0.9239219610986993 1106 88-89 1.2296718936337356 1106 90-91 1.1388260624670075 1106 92-93 0.8580222162048265 1106 94-95 0.4459936199785801 1106 96-97 0.47206279785313754 1106 98-99 0.5747175635221176 1106 100-101 0.5341344666416283 1107 1 -0.06388018177650778 1107 2 0.2729631081208801 1107 3 0.1525990576674623 1107 4 0.0710946731822446 1107 5 0.09550028306544078 1107 6 0.04817989974075232 1107 7 0.012848520149226772 1107 8 0.17175030849709572 1107 9 0.3498489014899988 1107 10-11 0.3053485866773471 1107 12-13 0.19516989019312803 1107 14-15 0.1810285869669528 1107 16-17 0.31426833817719313 1107 18-19 0.21176318236550884 1107 20-21 0.5264984030426874 1107 22-23 0.2409993655074203 1107 24-25 0.4316552183006266 1107 26-27 0.19267767887883025 1107 28-29 0.3980378368759716 1107 30-31 0.29241353213092225 1107 32-33 0.2422639066110861 1107 34-35 0.3227049571108864 1107 36-37 -0.031156156232640342 1107 38-39 0.17526950200306857 1107 40-41 0.27313829091028907 1107 42-43 0.4370017491681608 1107 44-45 0.2659016493858388 1107 46-47 0.2688378777639855 1107 48-49 -0.0010993592915724548 1107 50-51 0.07228672304128736 1107 52-53 0.3425784006449035 1107 54-55 0.5196649793229398 1107 56-57 0.5048337906573082 1107 58-59 0.6608909897795527 1107 60-61 0.7216132911399029 1107 62-63 0.6930469377081607 1107 64-65 0.6204001550662284 1107 66-67 0.8022797424407635 1107 68-69 0.8804124346728592 1107 70-71 0.5992470245341899 1107 72-73 0.5316558828245128 1107 74-75 0.7572767942240297 1107 76-77 0.4634766822625309 1107 78-79 0.677344631020425 1107 80-81 0.670029341317349 1107 82-83 0.34978357301434215 1107 84-85 0.39215025646124957 1107 86-87 0.529238350523034 1107 88-89 0.6016823169934824 1107 90-91 0.24856881014079235 1107 92-93 -0.302516562025108 1107 94-95 0.07898069786820727 1107 96-97 -0.4245008250972866 1107 98-99 -0.5098116004673194 1107 100-101 -0.10788975934061895 1108 1 -0.14456666733495283 1108 2 -0.10985362215818029 1108 3 -0.07635272458005815 1108 4 0.11238549847048063 1108 5 0.05564238742498162 1108 6 0.03776639718525132 1108 7 -0.07692419211585388 1108 8 -0.07652951558932841 1108 9 -0.11948508337509622 1108 10-11 -0.0835861588951019 1108 12-13 -0.2471068171062285 1108 14-15 -0.12153417799297017 1108 16-17 0.0014373446352848873 1108 18-19 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-1.879245807091511 2316 96-97 -1.9744580937478453 2316 98-99 -1.947805531655444 2316 100-101 -2.011547370802223 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 773.0 3 244.0 4 258.0 5 248.0 6 296.0 7 348.0 8 491.0 9 574.0 10 828.0 11 1314.0 12 2104.0 13 2991.0 14 3507.0 15 4792.0 16 7077.0 17 10619.0 18 9170.0 19 3836.0 20 3210.0 21 3033.0 22 3177.0 23 3381.0 24 3579.0 25 4184.0 26 4704.0 27 5406.0 28 6451.0 29 7569.0 30 9525.0 31 11710.0 32 14697.0 33 19646.0 34 27427.0 35 39281.0 36 55544.0 37 86597.0 38 89406.0 39 16341.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.58345450041466 20.90514502353334 15.452734067875106 19.058666408176894 2 15.188123119468788 22.475484442070485 41.38040952235315 20.955982916107576 3 18.130439745762892 29.826983508187755 32.16344921611662 19.879127529932735 4 11.41727195181844 19.314627652699873 40.28635803586045 28.981742359621233 5 11.151742655076262 40.816313337441976 33.75626532661584 14.27567868086592 6 25.580483432764755 40.33834691762597 19.330200651098053 14.75096899851122 7 24.757685150084434 38.18666643691629 21.377339146017853 15.678309266981424 8 26.370960270108963 33.83795616977908 20.544096322200172 19.24698723791178 9 28.887328282425727 16.636543610932193 21.515616955366895 32.960511151275185 10-11 23.672938335589755 28.942195741124287 27.295923038232083 20.088942885053868 12-13 26.02643663426809 27.81501896410017 27.92370841066729 18.23483599096445 14-15 23.244352828916618 27.53139949898656 29.008699885193394 20.215547786903432 16-17 19.16255436105433 31.210299467751696 28.941082202314668 20.686063968879306 18-19 19.162680095720784 28.454100765292385 34.310536824715086 18.072682314271745 20-21 20.9376709643791 26.83596896686899 34.342355226825305 17.884004841926604 22-23 20.63897171815722 26.927786159417717 34.040298637651475 18.392943484773593 24-25 19.543498673374454 27.62450036180697 34.516061645015675 18.3159393198029 26-27 19.31772418233935 27.70775052062807 34.78150849695161 18.193016800080976 28-29 18.880508941800763 28.14858206126598 34.7291616415699 18.24174735536336 30-31 19.644960729460763 27.760836278354578 34.61546744540136 17.9787355467833 32-33 19.166774346384116 28.002325881896883 34.29599000732222 18.53490976439678 34-35 19.345505369952814 28.2407616783283 34.168168789020186 18.245564162698695 36-37 19.703968833383943 27.791201762490232 34.14814049097327 18.35668891315256 38-39 19.136951711290695 28.81698315359891 33.792943786106164 18.253121349004235 40-41 19.22339735927967 28.633251712634838 33.79926046047857 18.344090467606918 42-43 18.999445452440817 28.858008905064686 32.99245707640373 19.150088566090762 44-45 19.5439132621142 28.78398764702272 32.700824716291784 18.9712743745713 46-47 19.60772490133899 29.126776032777528 31.93515562327377 19.330343442609713 48-49 19.699250549166557 29.63388896067537 31.24143946246285 19.425421027695222 50-51 19.93366965117343 29.8887136111814 30.631053586522878 19.546563151122285 52-53 19.804324628155417 30.38567602869015 30.113461996099844 19.696537347054583 54-55 19.724358194362036 29.82440460486998 30.109328512096344 20.341908688671637 56-57 20.29786235145647 30.37125653074673 29.276696917358326 20.05418420043847 58-59 20.20687449592378 29.97591202126892 29.684422712261178 20.13279077054612 60-61 20.61370542742507 29.869682357476517 30.004285772742058 19.512326442356358 62-63 20.381838445510493 29.77941453591984 30.15974544103633 19.67900157753334 64-65 20.065168536906043 29.605273784308512 30.35624102882728 19.973316649958168 66-67 20.222642700143123 29.9798723417545 29.62545755691798 20.172027401184398 68-69 19.937859769854988 29.729738461786994 29.91465141700187 20.417750351356155 70-71 19.96508012076548 30.026895415931094 30.01450866049627 19.993515802807163 72-73 20.0365093211851 30.03618622984718 29.793652332180976 20.13365211678675 74-75 19.62519992030113 30.61912018912326 29.56698743665825 20.188692453917362 76-77 19.313539102850648 30.306715425173824 29.742402128427326 20.637343343548203 78-79 19.35771882266401 30.298017346201355 29.5535932571031 20.790670574031534 80-81 19.442514266261167 30.350373125886758 29.41871651589731 20.788396091954766 82-83 19.726218932215055 30.552829641949604 29.207407255801527 20.51354417003381 84-85 19.51943995691976 30.09789983844911 29.01755519655358 21.365105008077546 86-87 19.260973608200345 30.145948612131225 29.201568904502306 21.39150887516612 88-89 19.537736844712576 29.209250432389005 29.586608110174655 21.66640461272376 90-91 19.274374219120244 29.2429236138038 30.04631424755504 21.436387919520918 92-93 19.654771265766882 29.46540711138878 29.753223391535705 21.126598231308638 94-95 19.25570385814069 29.870275323019403 29.639708717162293 21.23431210167761 96-97 19.45734739308981 30.335601117054907 28.63050921204119 21.576542277814095 98-99 18.999851357493842 30.41462642260572 28.46202399391643 22.123498225984005 100-101 19.55952214238778 30.296991306783294 28.682336719414852 21.461149831414076 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 817.0 1 551.5 2 308.5 3 335.5 4 308.0 5 324.0 6 494.5 7 950.0 8 1591.0 9 1864.5 10 1539.5 11 1180.0 12 1227.0 13 1295.5 14 1329.5 15 1456.0 16 1502.5 17 1578.0 18 1716.0 19 1822.5 20 2034.0 21 2283.5 22 2554.0 23 2894.0 24 3200.5 25 3802.5 26 5169.0 27 6638.5 28 7064.0 29 7493.5 30 8546.5 31 9655.0 32 11283.0 33 13034.0 34 15136.0 35 17031.5 36 18912.5 37 20247.0 38 18734.5 39 16829.0 40 15822.0 41 15027.0 42 14508.5 43 14710.0 44 15331.0 45 15080.0 46 14267.0 47 13797.0 48 15335.5 49 15184.5 50 13050.0 51 11637.5 52 10085.0 53 9089.0 54 8228.5 55 7384.5 56 6764.0 57 6114.5 58 5363.5 59 4699.0 60 4256.5 61 3850.5 62 3134.0 63 2159.5 64 1358.5 65 965.5 66 704.0 67 524.5 68 397.5 69 304.0 70 213.0 71 152.5 72 127.5 73 109.0 74 84.0 75 63.5 76 49.0 77 29.5 78 16.0 79 12.0 80 7.5 81 4.0 82 4.0 83 3.5 84 3.5 85 2.0 86 0.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023258668699646597 2 0.009906470001701329 3 0.011198618262792805 4 0.02132044630800938 5 0.060515610227784206 6 0.04393304087711024 7 0.015290421089582486 8 0.050393782182567626 9 0.029288693918073493 10-11 0.024227779895465207 12-13 0.038010694680440965 14-15 0.018090075655280688 16-17 0.01852079174231118 18-19 0.016367211307158715 20-21 0.013998272828491008 22-23 0.007429852501275996 24-25 0.001507506304606724 26-27 0.0 28-29 0.001507506304606724 30-31 0.0 32-33 6.460741305457389E-4 34-35 0.0 36-37 0.0 38-39 1.0767902175762314E-4 40-41 0.0 42-43 1.0767902175762314E-4 44-45 3.2303706527286944E-4 46-47 1.0767902175762314E-4 48-49 6.460741305457389E-4 50-51 1.0767902175762314E-4 52-53 5.383951087881157E-4 54-55 0.0018305433698795933 56-57 2.1535804351524628E-4 58-59 7.53753152303362E-4 60-61 0.0034457286962439404 62-63 0.002261259456910086 64-65 0.0018305433698795933 66-67 0.012167729458611415 68-69 0.015182742067824863 70-71 0.029611730983346362 72-73 0.01679792739418921 74-75 0.020566693155706018 76-77 0.03241138554904456 78-79 0.019705260981645035 80-81 0.048132522725657545 82-83 0.038010694680440965 84-85 0.020028298046917904 86-87 0.015075063046067238 88-89 0.01335219869794527 90-91 0.02648903935237529 92-93 0.03402657087540891 94-95 0.011198618262792805 96-97 0.018628470764068802 98-99 0.03058084217916497 100-101 0.0404873121808663 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 464343.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.867776013962086 #Duplication Level Percentage of deduplicated Percentage of total 1 83.50623359580823 44.14788853512785 2 9.201889792015365 9.72966896858865 3 2.605045668925905 4.131689127927503 4 1.1188405255198546 2.366024411941092 5 0.6270446962293841 1.6575229275498986 6 0.4436806265524961 1.4073844791787036 7 0.31629011982110317 1.170508864709192 8 0.2579623348581154 1.0910315951454026 9 0.19202299848491514 0.9136645986086888 >10 1.491357078386071 15.6480233372668 >50 0.15171276624901867 5.497541806167739 >100 0.08219478710255848 7.511800998556124 >500 0.004089292890674552 1.4260012486754217 >1k 0.0012267878672023655 1.910270926818682 >5k 4.089292890674551E-4 1.390978173738275 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6434 1.3856136519770945 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4528 0.975141221037035 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3004 0.6469355627197998 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1304 0.2808268887438811 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 963 0.20738979590518217 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 845 0.1819775467703831 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 734 0.15807280394019077 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 702 0.15118134654770288 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 668 0.1438591730681845 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 614 0.13222983871836122 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 538 0.1158626274112025 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 524 0.11284761480198904 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 507 0.10918652806222987 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 501 0.10789437980113839 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 465 0.10014149023458953 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.3071608703049255E-4 0.0 18-19 0.0 0.0 0.0 6.460741305457388E-4 0.0 20-21 0.0 0.0 0.0 6.460741305457388E-4 0.0 22-23 0.0 0.0 0.0 7.53753152303362E-4 0.0 24-25 0.0 0.0 0.0 0.0010767902175762313 0.0 26-27 0.0 0.0 0.0 0.0027996545656982013 0.0 28-29 0.0 0.0 0.0 0.006353062283699765 0.0 30-31 0.0 0.0 0.0 0.013459877719702891 0.0 32-33 0.0 0.0 0.0 0.018736149785826425 0.0 34-35 0.0 0.0 0.0 0.022504915547343234 0.0 36-37 0.0 0.0 0.0 0.027673508591709146 0.0 38-39 0.0 0.0 0.0 0.03811837370219859 0.0 40-41 0.0 0.0 0.0 0.0665456354462111 0.0 42-43 0.0 0.0 0.0 0.10617151545301641 0.0 44-45 0.0 0.0 0.0 0.1400904073066677 0.0 46-47 0.0 0.0 0.0 0.22634130373452382 0.0 48-49 0.0 0.0 0.0 0.31797615125026113 0.0 50-51 0.0 0.0 0.0 0.3820451691960469 0.0 52-53 0.0 0.0 0.0 0.4643119418188709 0.0 54-55 0.0 0.0 0.0 0.5255813051989585 0.0 56-57 0.0 0.0 0.0 0.6848385783784832 0.0 58-59 0.0 0.0 0.0 0.8916899791748771 0.0 60-61 0.0 0.0 0.0 1.096926194644907 0.0 62-63 0.0 0.0 0.0 1.3472799202313808 0.0 64-65 0.0 0.0 0.0 1.6699939484389772 0.0 66-67 0.0 0.0 0.0 2.0882192689455854 0.0 68-69 0.0 0.0 0.0 2.536379357500813 0.0 70-71 0.0 0.0 0.0 2.862431435382896 0.0 72-73 0.0 0.0 0.0 3.1221532358622825 0.0 74-75 0.0 0.0 0.0 3.4503588941795185 0.0 76-77 0.0 0.0 0.0 3.9034722177355965 0.0 78-79 0.0 0.0 0.0 4.2674273112763625 0.0 80-81 0.0 0.0 0.0 4.64311941818871 0.0 82-83 0.0 0.0 0.0 5.146088128818567 0.0 84-85 0.0 0.0 0.0 6.0725584320211565 0.0 86-87 0.0 0.0 0.0 7.181221640037645 0.0 88-89 0.0 0.0 0.0 8.204926099887368 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 155 0.0 45.97535 9 GGTATCA 3790 0.0 37.471745 1 ATTACTC 65 1.325673E-4 36.54451 3 CGTCACT 65 1.3273547E-4 36.536636 1 GTATCAA 6150 0.0 34.98606 1 TATCAAC 7425 0.0 28.984596 2 ATCAACG 7510 0.0 28.656542 3 TCAACGC 7515 0.0 28.64056 4 CAACGCA 7570 0.0 28.495237 5 AACGCAG 7600 0.0 28.382755 6 ATGGGGG 1015 0.0 28.086494 5 CTTTAGG 255 0.0 27.945803 2 ACTGTCC 105 6.884293E-5 27.150276 8 TACGTGA 70 0.008375333 27.150276 5 GCATTCC 265 0.0 26.89414 8 CATGGGG 2005 0.0 26.540918 4 ACGTGAT 90 8.925226E-4 26.3961 6 ACGCAGA 8355 0.0 25.70421 7 GTACATG 4405 0.0 25.662737 1 CGCAGAG 8365 0.0 25.616686 8 >>END_MODULE