FastQCFastQC Report
Thu 26 May 2016
SRR936881_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936881_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464343
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74141.596664534622036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58911.2686742343483157No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40110.8638011125396527No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13810.2974094580945551No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13280.2859954817882471No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12390.26682861591539014No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC9750.20997409242736512No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9490.20437478329596873No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA6680.1438591730681845No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA6580.14170559263303206No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC6240.13438341915351368No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA6120.13179912263133073No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5940.12792267784805628No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5940.12792267784805628No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT5880.1266305295869648No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA5360.115431911324172No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5230.1126322567584738No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4930.1061715154530164No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT4810.10358721893083346No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4670.10057220632162002No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35800.045.9108921
GTATCAA61100.039.262041
CGTCACT500.001614293138.002541
TATCAAC76250.031.3954432
ATCAACG77000.031.0862943
TCAACGC77200.031.0672784
CAACGCA78100.030.7225025
AACGCAG79900.030.0303826
ACGAAGC350.00838801727.14467276-77
CGCAGAG89200.026.8461448
ACGCAGA89250.026.8311067
TAACGGA458.942752E-426.39065454-55
GCAGAGT92000.025.8225089
GAGTACT57250.025.42635512-13
ACGTGGG950.001226515224.9962863
TACCTGG2701.8189894E-1224.6286242
CATTCCG1751.478129E-724.435479
GTACTTT62700.023.10262914-15
CATGGGT5400.022.8669744
AGTACTT58600.022.53078312-13
Produced by FastQC (version 0.11.5)