##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936881_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 464343 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13538268047542 33.0 31.0 34.0 31.0 34.0 2 32.22976334304598 33.0 31.0 34.0 30.0 34.0 3 32.29365361381565 34.0 31.0 34.0 30.0 34.0 4 36.008097462436176 37.0 35.0 37.0 35.0 37.0 5 35.939217345798255 37.0 35.0 37.0 35.0 37.0 6 36.00939607143857 37.0 35.0 37.0 35.0 37.0 7 35.950527950243675 37.0 35.0 37.0 35.0 37.0 8 35.93405736707563 37.0 35.0 37.0 35.0 37.0 9 37.56654671223643 39.0 37.0 39.0 35.0 39.0 10-11 37.50252076589935 39.0 37.0 39.0 35.0 39.0 12-13 37.39511094169612 39.0 37.0 39.0 34.0 39.0 14-15 38.44778751913995 40.0 38.0 41.0 34.0 41.0 16-17 38.40063810588293 40.0 38.0 41.0 34.0 41.0 18-19 38.66784036800382 40.0 38.0 41.0 34.0 41.0 20-21 38.74783619005778 40.0 38.0 41.0 35.0 41.0 22-23 38.64905360907777 40.0 38.0 41.0 34.0 41.0 24-25 38.52319944523768 40.0 38.0 41.0 34.0 41.0 26-27 38.29559614336816 40.0 38.0 41.0 34.0 41.0 28-29 38.14468184079441 40.0 37.0 41.0 34.0 41.0 30-31 37.90228236454517 40.0 37.0 41.0 33.5 41.0 32-33 37.50509321772914 40.0 35.5 41.0 33.0 41.0 34-35 37.22971058032532 39.0 35.0 41.0 33.0 41.0 36-37 36.95201392074394 39.0 35.0 41.0 32.5 41.0 38-39 36.687751295917025 39.0 35.0 41.0 32.0 41.0 40-41 36.38626618684894 39.0 35.0 41.0 31.0 41.0 42-43 35.92056087848853 39.0 35.0 41.0 30.0 41.0 44-45 35.27940875602734 39.0 35.0 41.0 27.5 41.0 46-47 34.37753018781375 38.5 35.0 40.0 19.0 41.0 48-49 33.58829249068038 38.0 34.0 40.0 4.5 41.0 50-51 33.148088805042825 38.0 33.5 40.0 2.0 41.0 52-53 32.85020448246232 38.0 33.0 40.0 2.0 41.0 54-55 32.550540225652156 38.0 33.0 40.0 2.0 41.0 56-57 32.27367377132852 38.0 33.0 40.0 2.0 41.0 58-59 31.928640035491007 37.5 32.0 40.0 2.0 41.0 60-61 31.619833829733622 37.0 31.5 40.0 2.0 41.0 62-63 31.261570649282966 36.0 31.0 39.5 2.0 41.0 64-65 30.902953204850725 36.0 31.0 39.0 2.0 40.0 66-67 30.532558905808855 35.5 30.0 39.0 2.0 40.0 68-69 30.206257012596293 35.0 30.0 38.5 2.0 40.0 70-71 29.769647652705004 35.0 29.0 38.0 2.0 40.0 72-73 29.28071705614169 35.0 29.0 37.0 2.0 39.5 74-75 28.78278126298878 34.0 28.0 37.0 2.0 39.0 76-77 27.40206162255057 32.5 26.0 35.0 2.0 37.5 78-79 28.070003208834848 34.0 27.0 36.0 2.0 38.0 80-81 27.78028849363509 34.0 26.5 35.5 2.0 37.0 82-83 27.467548773212904 34.0 26.0 35.0 2.0 37.0 84-85 27.145052256629256 34.0 26.0 35.0 2.0 36.0 86-87 26.831913047036352 34.0 25.5 35.0 2.0 36.0 88-89 26.431337394986034 33.0 25.0 35.0 2.0 35.5 90-91 26.004750798439947 33.0 23.5 35.0 2.0 35.0 92-93 25.561137994973542 33.0 21.0 35.0 2.0 35.0 94-95 25.033545245648153 32.0 18.5 34.5 2.0 35.0 96-97 24.43850451067422 32.0 4.5 34.0 2.0 35.0 98-99 23.726726794632416 32.0 2.0 34.0 2.0 35.0 100-101 20.71648544287305 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20634218712483232 1101 2 0.25184677315180437 1101 3 0.19618865151853981 1101 4 0.23788790445154717 1101 5 0.2040312769679744 1101 6 0.16555463511818402 1101 7 0.27066217750544297 1101 8 0.276971157485427 1101 9 0.41547196600821934 1101 10-11 0.4020008326368938 1101 12-13 0.40863729434774143 1101 14-15 0.6174264335664219 1101 16-17 0.512311314085629 1101 18-19 0.3818763309014841 1101 20-21 0.4381602662027717 1101 22-23 0.37303333868130295 1101 24-25 0.40459280766642536 1101 26-27 0.33345458828262764 1101 28-29 0.30816748271244165 1101 30-31 0.4660971744094624 1101 32-33 0.40480114298063796 1101 34-35 0.3159279940473212 1101 36-37 0.4268161310574783 1101 38-39 0.5886625284508824 1101 40-41 0.5213423333173353 1101 42-43 0.6073237564866858 1101 44-45 0.9335035750982357 1101 46-47 1.1609304113712255 1101 48-49 1.1539014183283527 1101 50-51 1.2008128475590425 1101 52-53 1.4093035507653084 1101 54-55 1.2847593744095889 1101 56-57 1.3363216927206523 1101 58-59 1.205857858315781 1101 60-61 1.270927713988609 1101 62-63 1.3004680354005913 1101 64-65 1.4248453163324655 1101 66-67 1.0691471689081062 1101 68-69 1.1946343540866842 1101 70-71 1.0594926435035141 1101 72-73 1.2570933125093937 1101 74-75 1.4702166683314317 1101 76-77 1.3192181778659382 1101 78-79 1.4735377507919942 1101 80-81 1.44763486997115 1101 82-83 1.5163429898946248 1101 84-85 1.4019287557679228 1101 86-87 0.9049757463027035 1101 88-89 1.134595172113091 1101 90-91 1.2770835210974916 1101 92-93 1.2866547368335155 1101 94-95 1.3993161059991799 1101 96-97 1.6076352254768302 1101 98-99 1.528230745260526 1101 100-101 1.9237429653647027 1102 1 0.30562450724379886 1102 2 0.2976091657843014 1102 3 0.3173054697128421 1102 4 0.1821078150652795 1102 5 0.23158877179824344 1102 6 0.1784191156537176 1102 7 0.15187222737811368 1102 8 0.16894444362741723 1102 9 0.3320578658940363 1102 10-11 0.28900058543168683 1102 12-13 0.43033249425042897 1102 14-15 0.4741200050538552 1102 16-17 0.40637799670945896 1102 18-19 0.3167838643820957 1102 20-21 0.38425285436282763 1102 22-23 0.2648320116209746 1102 24-25 0.30880373256704985 1102 26-27 0.21873175240725828 1102 28-29 0.4013756177257193 1102 30-31 0.5345553594523622 1102 32-33 0.5252862145328123 1102 34-35 0.5113966833496164 1102 36-37 0.540298036028652 1102 38-39 0.7457898862196046 1102 40-41 0.7734779116401782 1102 42-43 0.7295961609301074 1102 44-45 0.963799946675131 1102 46-47 0.8411302552003193 1102 48-49 0.9862825538242745 1102 50-51 1.002054955630875 1102 52-53 0.9900572519766158 1102 54-55 0.8224307013522107 1102 56-57 0.7508122341785395 1102 58-59 1.0666362015700201 1102 60-61 1.1387121667429803 1102 62-63 1.3761147909303375 1102 64-65 1.1612303914161437 1102 66-67 1.3470764525231083 1102 68-69 1.175570123895472 1102 70-71 1.164402805991891 1102 72-73 1.2800941875373546 1102 74-75 1.3753761941683038 1102 76-77 1.1842961259842326 1102 78-79 1.0458197687712705 1102 80-81 1.103513449051313 1102 82-83 0.6537792756952356 1102 84-85 0.5898204092568555 1102 86-87 0.5557699420813833 1102 88-89 0.6819339884317728 1102 90-91 0.8539251017982217 1102 92-93 0.8443045608469539 1102 94-95 0.7889223041792803 1102 96-97 0.8834514931490567 1102 98-99 1.276087319153298 1102 100-101 1.2771365435227509 1103 1 0.20250389391910772 1103 2 0.1085777436916544 1103 3 0.2894143132502549 1103 4 0.12854340517434082 1103 5 0.12586368811397364 1103 6 0.27123630602192605 1103 7 0.15590418936925943 1103 8 0.028076286840892806 1103 9 0.31051807537465237 1103 10-11 0.2568615075783214 1103 12-13 0.2579040308015621 1103 14-15 0.30295058490484195 1103 16-17 0.4045380226245854 1103 18-19 0.3779014542751895 1103 20-21 0.14612198296664758 1103 22-23 0.0662789395985115 1103 24-25 0.10962545808265389 1103 26-27 0.18358867930500367 1103 28-29 0.40176002545707235 1103 30-31 0.22781094740183505 1103 32-33 0.22699445338390944 1103 34-35 0.36785063000764495 1103 36-37 0.3378155827298741 1103 38-39 0.17695822657162097 1103 40-41 0.23684903786845268 1103 42-43 0.5266418363431242 1103 44-45 0.1687281401747569 1103 46-47 -0.05870669545296181 1103 48-49 0.156199409455418 1103 50-51 0.34418755470225193 1103 52-53 0.17376095568031502 1103 54-55 0.2834284337785178 1103 56-57 0.2674238311983004 1103 58-59 0.5157496882345214 1103 60-61 0.41291365686284465 1103 62-63 0.271573164756056 1103 64-65 0.0029321514852505004 1103 66-67 0.06841540080083419 1103 68-69 -0.0786939944502798 1103 70-71 0.06687014213269649 1103 72-73 0.15392098809885013 1103 74-75 0.15950533788928212 1103 76-77 0.18438142994708784 1103 78-79 0.19312999444884227 1103 80-81 0.25984861803954473 1103 82-83 0.2936302637094883 1103 84-85 0.42044129253079987 1103 86-87 0.2492576863854552 1103 88-89 0.28171047045315234 1103 90-91 0.4322103841651348 1103 92-93 0.6701829757049573 1103 94-95 0.7203908280967823 1103 96-97 0.5190394426145595 1103 98-99 0.6132863257400629 1103 100-101 0.8716338980201037 1104 1 0.06771962511422203 1104 2 0.1757713290295726 1104 3 0.022404003971907116 1104 4 0.07976142732020008 1104 5 -0.028783123646235254 1104 6 0.002500147182672663 1104 7 0.08971098585647042 1104 8 -0.00349301441912786 1104 9 0.00808203108293526 1104 10-11 0.12754115629920904 1104 12-13 0.10629654702912461 1104 14-15 -0.10758778852013506 1104 16-17 0.1745494773783136 1104 18-19 -0.05185799589663276 1104 20-21 0.11569815707889575 1104 22-23 -0.004759624739023138 1104 24-25 0.12389044471877497 1104 26-27 -0.07735442875151222 1104 28-29 -0.024352994779661685 1104 30-31 0.03729548348430711 1104 32-33 0.01715100168478756 1104 34-35 -0.02217609775104279 1104 36-37 -0.027300873932972536 1104 38-39 0.0863893071367201 1104 40-41 0.032717690025762636 1104 42-43 0.4728961318757712 1104 44-45 0.36007598286280285 1104 46-47 0.28887253326028883 1104 48-49 0.29980383862685045 1104 50-51 0.6189260044922449 1104 52-53 0.20827031039586075 1104 54-55 0.03783851396178761 1104 56-57 -0.023378001562299744 1104 58-59 -0.04055424456425527 1104 60-61 -0.07201188723795937 1104 62-63 0.0028217329118405132 1104 64-65 -0.2282629611531597 1104 66-67 -0.18283698560619044 1104 68-69 -0.11431446027692971 1104 70-71 0.03002401765770557 1104 72-73 -0.04884342579958556 1104 74-75 -0.06950573503931778 1104 76-77 -0.34718023481045535 1104 78-79 -0.11785129612007594 1104 80-81 0.2507688165886073 1104 82-83 0.23993037825702856 1104 84-85 0.21596630207642775 1104 86-87 -0.04820699479056856 1104 88-89 -0.12011083538876832 1104 90-91 -0.1284043542846547 1104 92-93 0.08175609521622462 1104 94-95 0.3818761278295071 1104 96-97 0.041960404813870866 1104 98-99 0.17945661974540883 1104 100-101 0.5304502401354121 1105 1 0.10453745148502236 1105 2 0.11533255696004119 1105 3 0.13603922626347753 1105 4 0.11676578203972099 1105 5 0.04949780239527257 1105 6 0.0658516311591697 1105 7 0.09462529042856715 1105 8 0.03157431514059539 1105 9 0.08786594516511315 1105 10-11 0.12053737551306654 1105 12-13 0.04106184414725789 1105 14-15 0.15059614278004574 1105 16-17 0.1650437057607519 1105 18-19 -0.16449242185563406 1105 20-21 -0.07956115319665713 1105 22-23 -0.029878177852971533 1105 24-25 -0.12755694352564007 1105 26-27 0.054684326100023384 1105 28-29 0.019274909861309197 1105 30-31 0.014463696184016328 1105 32-33 -0.11531282257681141 1105 34-35 0.23231672449461627 1105 36-37 0.055887182513373546 1105 38-39 0.07706647678425327 1105 40-41 0.0642409809597666 1105 42-43 0.3001421842902374 1105 44-45 0.20978435296374442 1105 46-47 -0.2261914244408203 1105 48-49 -0.04045256611096448 1105 50-51 0.11108463967867266 1105 52-53 0.31051654216450686 1105 54-55 0.25703084621182626 1105 56-57 0.10705732690694703 1105 58-59 -0.006265312152081037 1105 60-61 0.19675035368492644 1105 62-63 0.3191630510933372 1105 64-65 -0.15832200454969936 1105 66-67 0.07816101280114296 1105 68-69 -0.051807734495124436 1105 70-71 -0.14661474149832188 1105 72-73 -0.10737028229472045 1105 74-75 -0.1050860175580226 1105 76-77 -0.22357650711314747 1105 78-79 0.10890978258771256 1105 80-81 0.2983191971781807 1105 82-83 0.13492926626110346 1105 84-85 0.022920222400124146 1105 86-87 0.2394053706041177 1105 88-89 0.3138886193745165 1105 90-91 0.3834204668992278 1105 92-93 0.3211239793390739 1105 94-95 0.44093207915995336 1105 96-97 0.3043143208330683 1105 98-99 0.2781050066339006 1105 100-101 0.4009063019480408 1106 1 -0.09620786131438663 1106 2 0.04790141518924429 1106 3 0.142554409868616 1106 4 0.033793002597846566 1106 5 0.08048526621381313 1106 6 -0.05385226080576899 1106 7 -0.15768679291737442 1106 8 -0.11704448610596785 1106 9 -0.12220334169459335 1106 10-11 -0.3284202959643494 1106 12-13 -0.03483348932726926 1106 14-15 0.12010034335894204 1106 16-17 0.16048005161010082 1106 18-19 -0.02726762785041359 1106 20-21 -0.09682487693673636 1106 22-23 -0.0024811248137339703 1106 24-25 0.0951166013998801 1106 26-27 0.13577869540805665 1106 28-29 0.23725116877429997 1106 30-31 -0.05542576757401463 1106 32-33 -0.1590280908995041 1106 34-35 -0.381456938430361 1106 36-37 -0.0477077667065231 1106 38-39 -0.13672937407250174 1106 40-41 -0.05514774151963309 1106 42-43 -0.05925188346363797 1106 44-45 -0.35178715773184877 1106 46-47 -0.40526064070255785 1106 48-49 -0.5617555019777569 1106 50-51 -0.39275286397730014 1106 52-53 -0.3118751151731445 1106 54-55 -0.544349343999265 1106 56-57 -0.2148467002026706 1106 58-59 -0.2042825178846357 1106 60-61 -0.383269918016385 1106 62-63 -0.2010355308961138 1106 64-65 -0.20922565849864938 1106 66-67 0.039397687435293705 1106 68-69 0.07008217302153952 1106 70-71 -0.2076870237288162 1106 72-73 -0.36799527599132986 1106 74-75 -0.4331194608224749 1106 76-77 -0.32598894042328297 1106 78-79 0.009732248877185157 1106 80-81 0.18654749082537236 1106 82-83 0.2807317129848528 1106 84-85 0.20477301555817107 1106 86-87 -0.24597580797847485 1106 88-89 0.3186186830135398 1106 90-91 0.39678365308622787 1106 92-93 0.41243740404634366 1106 94-95 0.4190542741515344 1106 96-97 0.6692326793295358 1106 98-99 0.9525777911184861 1106 100-101 0.8820364745096363 1107 1 0.09339971584940798 1107 2 0.03049345250391866 1107 3 0.04179526563120817 1107 4 0.18688312420371034 1107 5 -0.08458695957804707 1107 6 0.0997019144875324 1107 7 0.15031396860662483 1107 8 0.14743041929847323 1107 9 0.20106359626500137 1107 10-11 0.11641085988629385 1107 12-13 0.26945484269719344 1107 14-15 0.24583509503072776 1107 16-17 0.26648657215934435 1107 18-19 0.16166171700097465 1107 20-21 0.13767097037769815 1107 22-23 0.05848839042864995 1107 24-25 0.00893945187940659 1107 26-27 0.15871365649893932 1107 28-29 0.23481786161835316 1107 30-31 0.07828713787802144 1107 32-33 0.3915775586566639 1107 34-35 0.32583822351247704 1107 36-37 0.14829542217523084 1107 38-39 0.3242306221198774 1107 40-41 0.09068410446214159 1107 42-43 0.1081525095779412 1107 44-45 0.22206877144642334 1107 46-47 0.2373049812483714 1107 48-49 0.822665382817938 1107 50-51 0.5268111635327628 1107 52-53 0.3287272027155339 1107 54-55 0.45701901943807144 1107 56-57 0.8353603391348017 1107 58-59 0.7588549609949666 1107 60-61 1.0136744669339954 1107 62-63 0.6487598095289115 1107 64-65 0.6604763952363903 1107 66-67 0.26715285746932693 1107 68-69 0.5246703551209677 1107 70-71 0.31326678508106554 1107 72-73 0.5150393971482927 1107 74-75 0.4829463158130167 1107 76-77 0.35326666986680877 1107 78-79 0.6965436051038338 1107 80-81 0.8488889391488321 1107 82-83 0.8422783206334081 1107 84-85 0.5679545200440224 1107 86-87 0.6238809303190429 1107 88-89 0.9979337873661258 1107 90-91 0.8090120626909503 1107 92-93 0.6982052344168714 1107 94-95 0.7210877812075296 1107 96-97 0.8024220370316364 1107 98-99 1.034733142014467 1107 100-101 0.7894518144587117 1108 1 0.03757488984800972 1108 2 0.020832733265677916 1108 3 0.14482092629761922 1108 4 0.15387951876782324 1108 5 0.12311520236281126 1108 6 0.1918122170536023 1108 7 -0.010240185568683557 1108 8 0.12070661153556017 1108 9 0.24947944961752455 1108 10-11 0.18269211029900845 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66-67 0.3597214436858245 2316 68-69 0.45428846169007286 2316 70-71 0.4323672181560916 2316 72-73 0.5377806372216547 2316 74-75 0.33580163418557873 2316 76-77 0.36114556250069185 2316 78-79 0.32583954610576527 2316 80-81 0.6437472535171267 2316 82-83 0.4967881584463285 2316 84-85 0.5499929494508784 2316 86-87 0.418614411531653 2316 88-89 5.408798098791578E-4 2316 90-91 -0.0775362045288226 2316 92-93 -0.015353086673208338 2316 94-95 0.0583245512936621 2316 96-97 0.4041466550901909 2316 98-99 -0.03369223176481029 2316 100-101 -0.4248963164048476 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 5.0 5 27.0 6 43.0 7 85.0 8 203.0 9 467.0 10 888.0 11 1514.0 12 2470.0 13 3512.0 14 4430.0 15 6607.0 16 9184.0 17 13640.0 18 11467.0 19 5269.0 20 3539.0 21 3085.0 22 3288.0 23 3423.0 24 3829.0 25 4361.0 26 5059.0 27 5985.0 28 7375.0 29 8848.0 30 10787.0 31 13255.0 32 16609.0 33 21657.0 34 29989.0 35 40470.0 36 59117.0 37 92243.0 38 67587.0 39 4021.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.41894666095097 21.01335791943027 16.716463565687746 18.85123185393101 2 15.434498413799194 22.739206188875272 41.102953370219055 20.72334202710648 3 18.003286363744042 29.103701358693897 33.32924153050654 19.563770747055518 4 11.395289237866137 19.598312447102455 40.26803577543228 28.738362539599134 5 10.740767062279392 40.21983749082037 34.31041277676201 14.728982670138238 6 25.440030322412525 40.366065602367215 18.747348404089216 15.446555671131037 7 23.66117288297659 38.6298490555473 21.461721184555383 16.247256876920723 8 26.211873550371166 33.36456024964304 20.602442590929552 19.821123609056237 9 28.27672647159535 15.934987713823617 21.960490413336693 33.82779540124434 10-11 23.419535897513214 28.61656622743348 27.473217401043303 20.490680474010002 12-13 26.09558020687294 27.58801144843359 28.442229128036818 17.874179216656653 14-15 22.988178996991447 26.852025334720235 30.464656514688492 19.69513915359982 16-17 18.508408654809053 30.189213577032497 30.474455305668442 20.82792246249001 18-19 18.422412790572466 26.638232165085217 37.114992828561704 17.82436221578061 20-21 19.687231135178905 25.2403160208168 37.49576007916672 17.57669276483757 22-23 19.421427533071668 25.125745021078643 37.43362784211745 18.01919960373224 24-25 18.667261110554715 25.83644824084454 37.81675405637956 17.679536592221183 26-27 18.55976947007623 25.733678065736783 38.165904443365214 17.54064802082178 28-29 18.06145248388851 26.208133005270895 38.16019425316442 17.570220257676176 30-31 18.877595521322498 25.95526706558971 37.725040299698385 17.44209711338941 32-33 18.220691690006923 26.126227988279798 37.51788881315195 18.135191508561324 34-35 18.61684142971898 26.184092362757706 37.41630648033889 17.78275972718443 36-37 19.08431555198983 26.005201406425837 37.09475067171372 17.81573236987061 38-39 18.443348119716937 27.05746658525002 36.85331110529564 17.64587418973741 40-41 18.51517644474499 26.742480692943506 36.71474227825421 18.027600584057303 42-43 18.448431439690058 26.946890552888703 36.00786487574918 18.59681313167206 44-45 19.01450005706988 26.799585651124275 35.51469495609926 18.671219335706578 46-47 19.177588046728538 27.208853828486145 34.36436329727151 19.2491948275138 48-49 19.273841674312024 28.20157472853592 33.04334810718147 19.48123548997058 50-51 19.573630889308145 28.33081349770415 32.24355826635309 19.85199734663462 52-53 19.575077044340066 29.111454248260443 31.586241205315897 19.72722750208359 54-55 19.51422793555487 28.231786239616824 31.68771119002957 20.566274634798734 56-57 20.164020571487644 28.567490502485292 30.847970848444646 20.420518077582418 58-59 19.863527020013308 28.55498437570665 31.040872979125254 20.54061562515479 60-61 20.295713542715436 28.492175360650567 31.434881534755654 19.777229561878343 62-63 20.18312564878986 28.461253515848778 31.386891968143072 19.968728867218292 64-65 19.805854514717048 28.273741903874832 31.84416675191265 20.07623682949547 66-67 20.179656129295555 28.467269612335155 31.012326322896467 20.340747935472827 68-69 20.18068656587845 28.200564241719427 31.091333936339748 20.52741525606237 70-71 19.92990012652435 28.63294478692761 31.44372358467709 19.993431501870944 72-73 20.013761378980625 28.30084657246906 31.366037605821557 20.31935444272876 74-75 19.64218222540474 29.045262925534495 30.845012033143636 20.46754281591713 76-77 19.037543367726013 28.74739147569792 31.120744794257693 21.09432036231837 78-79 18.87121593085974 28.95259098295076 30.969684374202483 21.20650871198702 80-81 19.159459180069433 28.743657555369868 30.700662457035055 21.39622080752565 82-83 18.913048160592748 29.07792711294908 30.74179374515379 21.267230981304387 84-85 18.74262343099839 28.39569854493138 30.37444971872119 22.487228305349035 86-87 18.252419657048343 28.482389376337974 30.458173422754037 22.807017543859647 88-89 18.502203117529753 27.750558855326464 30.798097092229433 22.949140934914354 90-91 17.977363545423124 27.80820929513858 31.363797393932675 22.850629765505627 92-93 18.074647726563022 27.98579061508676 31.223820425075537 22.715741233274684 94-95 17.792020123055586 28.051892674165437 31.13366627686861 23.02242092591037 96-97 18.025572219877155 28.453886443320727 30.245061723077455 23.275479613724663 98-99 17.2836794204172 28.542816162503716 29.966145059375364 24.207359357703723 100-101 17.17005085707579 27.905168728551573 30.649275693160515 24.275504721212126 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1120.0 1 777.0 2 430.5 3 442.5 4 395.0 5 378.5 6 576.0 7 1068.0 8 1623.5 9 1885.5 10 1703.5 11 1378.0 12 1290.0 13 1348.5 14 1424.0 15 1503.0 16 1605.5 17 1728.0 18 1889.5 19 2050.5 20 2207.0 21 2439.5 22 2756.0 23 3081.5 24 3436.5 25 4067.5 26 5326.5 27 6737.0 28 7279.0 29 7929.5 30 8952.5 31 9784.0 32 11208.0 33 12657.5 34 14927.5 35 17054.0 36 18569.0 37 19700.5 38 18564.0 39 16851.0 40 15826.5 41 15240.0 42 14488.5 43 14550.0 44 15281.0 45 15136.5 46 14456.5 47 13920.0 48 15364.5 49 15123.5 50 12766.0 51 11252.0 52 9855.5 53 9110.0 54 8251.0 55 7195.0 56 6481.5 57 5869.5 58 5059.0 59 4314.5 60 3830.0 61 3366.0 62 2818.5 63 2070.0 64 1337.0 65 919.0 66 681.5 67 481.5 68 334.5 69 275.5 70 236.5 71 193.0 72 167.0 73 149.0 74 116.0 75 94.0 76 72.5 77 33.5 78 11.5 79 8.0 80 6.0 81 5.5 82 4.5 83 2.5 84 2.0 85 1.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02670439739589054 2 0.005599309131396403 3 0.0 4 4.3071608703049255E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.010552544132247068 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.1535804351524628E-4 20-21 0.0013998272828491007 22-23 0.002261259456910086 24-25 0.009368074892913213 26-27 0.004630197935577795 28-29 1.0767902175762314E-4 30-31 0.0020459014133948393 32-33 0.0035534077180015638 34-35 0.0 36-37 0.009798790979943704 38-39 0.013890593806733385 40-41 0.0010767902175762313 42-43 0.0 44-45 0.0 46-47 3.2303706527286944E-4 48-49 0.0019382223916372164 50-51 0.005814667174911649 52-53 0.0 54-55 0.0021535804351524627 56-57 0.002368938478667709 58-59 4.3071608703049255E-4 60-61 0.0018305433698795933 62-63 0.0036610867397591867 64-65 1.0767902175762314E-4 66-67 0.0024766175004253322 68-69 6.460741305457389E-4 70-71 0.0011844692393338544 72-73 0.0 74-75 1.0767902175762314E-4 76-77 0.0 78-79 0.0024766175004253322 80-81 0.002368938478667709 82-83 0.013567556741460516 84-85 0.007537531523033619 86-87 0.004522518913820172 88-89 2.1535804351524628E-4 90-91 7.53753152303362E-4 92-93 4.3071608703049255E-4 94-95 0.0 96-97 0.002368938478667709 98-99 0.0019382223916372164 100-101 5.383951087881157E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 464343.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.06768767173335 #Duplication Level Percentage of deduplicated Percentage of total 1 82.58066365532005 41.346228756190264 2 9.547328707502615 9.56025343653229 3 2.788298518780292 4.188109781215451 4 1.2263572286267954 2.456034827874355 5 0.6957815063372236 1.7418085573530133 6 0.4906653007908308 1.4739886218811449 7 0.3591937505887099 1.2588800362679808 8 0.25630969046011515 1.0266266823356558 9 0.20297669935871454 0.9146316589318294 >10 1.591590307358638 15.917866712660246 >50 0.1630757169958074 5.586732137679835 >100 0.0904053709600365 8.338082931452655 >500 0.004758177418949289 1.5855351984718153 >1k 0.001730246334163378 1.7237091441930312 >5k 8.65123167081689E-4 2.881511516960457 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7414 1.596664534622036 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5891 1.2686742343483157 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4011 0.8638011125396527 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1381 0.2974094580945551 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1328 0.2859954817882471 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1239 0.26682861591539014 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 975 0.20997409242736512 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 949 0.20437478329596873 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 668 0.1438591730681845 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 658 0.14170559263303206 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 624 0.13438341915351368 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 612 0.13179912263133073 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 594 0.12792267784805628 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 594 0.12792267784805628 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 588 0.1266305295869648 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 536 0.115431911324172 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 523 0.1126322567584738 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 493 0.1061715154530164 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 481 0.10358721893083346 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 467 0.10057220632162002 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.3071608703049255E-4 0.0 18-19 0.0 0.0 0.0 6.460741305457388E-4 0.0 20-21 0.0 0.0 0.0 7.53753152303362E-4 0.0 22-23 0.0 0.0 0.0 9.691111958186082E-4 0.0 24-25 0.0 0.0 0.0 0.0012921482610914776 0.0 26-27 0.0 0.0 0.0 0.003015012609213448 0.0 28-29 0.0 0.0 0.0 0.006568420327215012 0.0 30-31 0.0 0.0 0.0 0.013782914784975762 0.0 32-33 0.0 0.0 0.0 0.019382223916372163 0.0 34-35 0.0 0.0 0.0 0.02336634772140422 0.0 36-37 0.0 0.0 0.0 0.028319582722254884 0.0 38-39 0.0 0.0 0.0 0.0386567688109867 0.0 40-41 0.0 0.0 0.0 0.06751474664202971 0.0 42-43 0.0 0.0 0.0 0.10821741686641126 0.0 44-45 0.0 0.0 0.0 0.14299774089412354 0.0 46-47 0.0 0.0 0.0 0.23129453873537448 0.0 48-49 0.0 0.0 0.0 0.32465225059923375 0.0 50-51 0.0 0.0 0.0 0.3888289475667771 0.0 52-53 0.0 0.0 0.0 0.4706650041025707 0.0 54-55 0.0 0.0 0.0 0.5326881206349616 0.0 56-57 0.0 0.0 0.0 0.6933452210973354 0.0 58-59 0.0 0.0 0.0 0.8991198316761533 0.0 60-61 0.0 0.0 0.0 1.1058635534507897 0.0 62-63 0.0 0.0 0.0 1.3548174517544143 0.0 64-65 0.0 0.0 0.0 1.6769930848532226 0.0 66-67 0.0 0.0 0.0 2.094033936120497 0.0 68-69 0.0 0.0 0.0 2.5437015309803312 0.0 70-71 0.0 0.0 0.0 2.868999855710111 0.0 72-73 0.0 0.0 0.0 3.1274295079284062 0.0 74-75 0.0 0.0 0.0 3.454450697006308 0.0 76-77 0.0 0.0 0.0 3.9098252800192963 0.0 78-79 0.0 0.0 0.0 4.273349657473032 0.0 80-81 0.0 0.0 0.0 4.647534258080773 0.0 82-83 0.0 0.0 0.0 5.1493184994712955 0.0 84-85 0.0 0.0 0.0 6.074927370499824 0.0 86-87 0.0 0.0 0.0 7.184236652646858 0.0 88-89 0.0 0.0 0.0 8.207402717387794 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3580 0.0 45.910892 1 GTATCAA 6110 0.0 39.26204 1 CGTCACT 50 0.0016142931 38.00254 1 TATCAAC 7625 0.0 31.395443 2 ATCAACG 7700 0.0 31.086294 3 TCAACGC 7720 0.0 31.067278 4 CAACGCA 7810 0.0 30.722502 5 AACGCAG 7990 0.0 30.030382 6 ACGAAGC 35 0.008388017 27.144672 76-77 CGCAGAG 8920 0.0 26.846144 8 ACGCAGA 8925 0.0 26.831106 7 TAACGGA 45 8.942752E-4 26.390654 54-55 GCAGAGT 9200 0.0 25.822508 9 GAGTACT 5725 0.0 25.426355 12-13 ACGTGGG 95 0.0012265152 24.996286 3 TACCTGG 270 1.8189894E-12 24.628624 2 CATTCCG 175 1.478129E-7 24.43547 9 GTACTTT 6270 0.0 23.102629 14-15 CATGGGT 540 0.0 22.866974 4 AGTACTT 5860 0.0 22.530783 12-13 >>END_MODULE