FastQCFastQC Report
Thu 26 May 2016
SRR936880_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936880_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483039
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66261.3717318891435266No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47910.9918453789445572No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30400.629348768939982No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13610.28175778767345905No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10650.22047909175035557No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8570.17741838650709363No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7760.1606495541767849No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT7220.14947033262324574No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC6810.14098240514741045No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA6530.13518577174927904No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA5590.11572564534126643No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC5380.11137817029266787No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT5290.10951496670041136No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT5020.10392535592364177No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA4900.10144108446729974No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35350.041.0092281
GTATCAA57800.036.8401451
TATCAAC72150.029.1866022
ATCAACG72900.028.8211213
TCAACGC72850.028.7756544
CAACGCA73600.028.4824225
AACGCAG75100.028.0343156
GTGTAGC1903.9108272E-1027.517581
GTAGTAC700.00835650227.1630233
GTACATG45950.026.894221
CATGGGA19700.026.3012524
TACATGG47550.026.0919252
ATATGAG1658.3289706E-825.9283393
ACGCAGA83400.025.1847327
CGCAGAG83600.025.1813248
ACATGGG47850.025.0342583
CATTCCG1751.4777288E-724.4365929
GAGTACT43650.024.3887712-13
GCAGAGT84850.024.135799
ATGGGAT9100.022.9840975
Produced by FastQC (version 0.11.5)