##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936880_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 483039 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.866720906593464 33.0 31.0 34.0 30.0 34.0 2 31.925515331060225 33.0 31.0 34.0 30.0 34.0 3 31.898670707748234 33.0 31.0 34.0 30.0 34.0 4 35.554286506886605 37.0 35.0 37.0 33.0 37.0 5 35.58617213102876 37.0 35.0 37.0 33.0 37.0 6 35.684151797266885 37.0 35.0 37.0 33.0 37.0 7 35.65264916497426 37.0 35.0 37.0 33.0 37.0 8 35.620546167079674 37.0 35.0 37.0 33.0 37.0 9 37.23620038961657 39.0 37.0 39.0 34.0 39.0 10-11 37.11470191847863 39.0 37.0 39.0 33.0 39.0 12-13 37.03009280824116 39.0 37.0 39.0 33.0 39.0 14-15 38.07825972644031 40.0 38.0 41.0 33.0 41.0 16-17 38.05194508103901 40.0 38.0 41.0 33.0 41.0 18-19 38.13331739259149 40.0 38.0 41.0 33.5 41.0 20-21 38.13342193901528 40.0 38.0 41.0 33.5 41.0 22-23 38.032035715542634 40.0 38.0 41.0 33.0 41.0 24-25 37.95599320137711 40.0 37.5 41.0 33.0 41.0 26-27 37.90318380089393 40.0 37.0 41.0 33.0 41.0 28-29 37.804938110587344 40.0 37.0 41.0 33.0 41.0 30-31 37.5132856767259 39.5 36.5 41.0 33.0 41.0 32-33 37.17750637112117 39.0 35.5 41.0 32.0 41.0 34-35 36.95486285786448 39.0 35.0 41.0 32.0 41.0 36-37 36.73237564668692 39.0 35.0 41.0 31.0 41.0 38-39 36.49984887348641 39.0 35.0 41.0 31.0 41.0 40-41 36.26459664747567 39.0 35.0 41.0 30.0 41.0 42-43 35.89205633499573 39.0 35.0 41.0 30.0 41.0 44-45 35.4075882071634 39.0 35.0 40.0 28.0 41.0 46-47 34.71046851289441 38.0 35.0 40.0 23.5 41.0 48-49 34.16000364359813 38.5 34.0 40.0 18.0 41.0 50-51 33.32769714246676 38.0 33.5 39.5 13.0 40.5 52-53 33.44962725577024 38.0 33.5 40.0 9.0 40.5 54-55 33.79234078407748 38.5 34.0 40.0 7.5 41.0 56-57 33.678418305768275 38.0 34.0 41.0 4.5 41.0 58-59 33.46701094528599 38.0 34.0 40.0 2.0 41.0 60-61 33.17696914741874 38.0 34.0 40.0 2.0 41.0 62-63 32.86484631675703 37.5 33.0 40.0 2.0 41.0 64-65 32.556294626313814 37.0 33.0 40.0 2.0 41.0 66-67 32.1952968600879 36.5 33.0 39.5 2.0 41.0 68-69 31.78235401282298 36.0 32.0 39.0 2.0 41.0 70-71 31.38073323272034 35.5 32.0 39.0 2.0 40.5 72-73 31.001906678342742 35.0 31.0 38.5 2.0 40.0 74-75 30.588892408273452 35.0 31.0 37.5 2.0 39.5 76-77 30.156935568349553 35.0 31.0 37.0 2.0 39.0 78-79 29.706788685799697 35.0 30.0 36.5 2.0 39.0 80-81 29.277804690718554 35.0 30.0 36.0 2.0 38.5 82-83 28.866106049408017 34.5 29.0 35.5 2.0 37.0 84-85 28.49181639577757 34.0 29.0 35.0 2.0 37.0 86-87 28.138274549259997 34.0 29.0 35.0 2.0 36.0 88-89 27.77400789584278 34.0 28.0 35.0 2.0 36.0 90-91 27.38535294251603 34.0 27.0 35.0 2.0 36.0 92-93 27.03434505288393 34.0 26.0 35.0 2.0 35.0 94-95 26.67480576102551 34.0 25.0 35.0 2.0 35.0 96-97 26.23437134475684 34.0 24.5 35.0 2.0 35.0 98-99 25.75980510890425 34.0 21.5 35.0 2.0 35.0 100-101 23.912051614879957 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14531246976348555 1101 2 0.3784335046760816 1101 3 0.20564462771296377 1101 4 0.2180629209753775 1101 5 0.022285072696959674 1101 6 0.3067387537913646 1101 7 0.20868298613171277 1101 8 0.2510933518634815 1101 9 0.31225016965634467 1101 10-11 0.20922930117602334 1101 12-13 0.4942776492820258 1101 14-15 0.5424872237976217 1101 16-17 0.44990583253070326 1101 18-19 0.5156672668544999 1101 20-21 0.42170855225985093 1101 22-23 0.3790937238749734 1101 24-25 0.4282427357086789 1101 26-27 0.44799564081263554 1101 28-29 0.35657493363037673 1101 30-31 0.3555365901900842 1101 32-33 0.4482899176830131 1101 34-35 0.7129163345399974 1101 36-37 0.656830387876802 1101 38-39 0.73202730467127 1101 40-41 0.8259827649046798 1101 42-43 0.724906396663556 1101 44-45 0.8324546971746756 1101 46-47 0.9919486292147965 1101 48-49 1.2552998064605205 1101 50-51 1.2807603805811354 1101 52-53 1.282788612042772 1101 54-55 1.382663431826181 1101 56-57 1.5366451427770684 1101 58-59 1.5659726722014966 1101 60-61 1.569854918171984 1101 62-63 1.4385922072101565 1101 64-65 1.3856562515346553 1101 66-67 1.2759034960946067 1101 68-69 1.44575711732341 1101 70-71 1.3597255295855497 1101 72-73 1.293151790927464 1101 74-75 1.2938329843827994 1101 76-77 1.4481692124635046 1101 78-79 1.3939550081158139 1101 80-81 1.3478531007846755 1101 82-83 1.3451518358334447 1101 84-85 1.4976045366088506 1101 86-87 1.5733646202833107 1101 88-89 1.5594508820278676 1101 90-91 1.8005541636215234 1101 92-93 1.9186328891120112 1101 94-95 1.8687319781715495 1101 96-97 1.9437225063728576 1101 98-99 2.083929859888105 1101 100-101 1.9573709053556883 1102 1 0.17079831176868154 1102 2 0.15966672335216003 1102 3 0.29098083473463277 1102 4 0.22548298744756323 1102 5 0.21511609111468744 1102 6 0.13929561073103258 1102 7 0.11170688999377631 1102 8 0.23398184404791778 1102 9 0.42212520775448326 1102 10-11 0.28157222798405 1102 12-13 0.32928730081103197 1102 14-15 0.18475293837359885 1102 16-17 0.2894945177457231 1102 18-19 0.17329378896592829 1102 20-21 0.16799307643221084 1102 22-23 0.2058034232987893 1102 24-25 0.19381399011717804 1102 26-27 0.19533965613896953 1102 28-29 0.10344000090080385 1102 30-31 0.14661364277277755 1102 32-33 -0.16429008841269166 1102 34-35 0.2339882348026876 1102 36-37 0.16688829705083918 1102 38-39 0.05667970449130166 1102 40-41 0.09044191644094468 1102 42-43 0.22072467216028002 1102 44-45 0.5672245118362937 1102 46-47 0.960118539666027 1102 48-49 1.1617779417560925 1102 50-51 0.9357907865258994 1102 52-53 0.8530179991343942 1102 54-55 0.6723889075086831 1102 56-57 0.9337638348134689 1102 58-59 0.698971467574566 1102 60-61 0.6888771384590626 1102 62-63 0.4959643788767423 1102 64-65 0.6886380660221079 1102 66-67 0.32284947655933394 1102 68-69 0.6627623131841389 1102 70-71 0.7534807000475148 1102 72-73 0.6377567313492278 1102 74-75 0.7621552873972668 1102 76-77 0.6724910655570753 1102 78-79 0.7770100436015106 1102 80-81 0.7887882831477775 1102 82-83 0.7681373512831478 1102 84-85 0.6390974031855343 1102 86-87 0.9813412824507637 1102 88-89 0.979666118381477 1102 90-91 0.8493397400282525 1102 92-93 1.122499668984581 1102 94-95 1.1615778730061699 1102 96-97 0.948824028847131 1102 98-99 0.9241553283138195 1102 100-101 0.8537475907448737 1103 1 0.30264168741681274 1103 2 0.2111954199371766 1103 3 0.2232115993809174 1103 4 0.24907199751967823 1103 5 0.22477101691480073 1103 6 0.1734752400295605 1103 7 0.1892860067468547 1103 8 0.17477688546874504 1103 9 0.16476183687422008 1103 10-11 0.21087373582123092 1103 12-13 0.23316715062329507 1103 14-15 0.4294695484218778 1103 16-17 0.448040915028038 1103 18-19 0.35077456851178823 1103 20-21 0.1842485147777566 1103 22-23 0.22910934141070527 1103 24-25 0.36349335122332604 1103 26-27 0.5539247923210979 1103 28-29 0.5123350482814573 1103 30-31 0.44552299374720405 1103 32-33 0.49382422114091895 1103 34-35 0.48348462910509227 1103 36-37 0.7128624313854175 1103 38-39 0.9491762775886272 1103 40-41 1.0199102995388571 1103 42-43 1.3180576479037711 1103 44-45 1.358424821747498 1103 46-47 1.7618021918503004 1103 48-49 2.267371242906343 1103 50-51 2.3565697364037206 1103 52-53 2.4724470473494264 1103 54-55 2.4159940091237857 1103 56-57 2.428864853944525 1103 58-59 2.5364886020879496 1103 60-61 2.492691430133185 1103 62-63 2.450931897799208 1103 64-65 2.557912158637876 1103 66-67 2.6211419850520983 1103 68-69 2.3965664731459952 1103 70-71 2.3930211970974007 1103 72-73 2.506607675982405 1103 74-75 2.601135560357516 1103 76-77 2.7429750038132283 1103 78-79 2.752100931020305 1103 80-81 2.6151545743451052 1103 82-83 2.706763014314319 1103 84-85 2.5697428528547626 1103 86-87 2.5207059644937964 1103 88-89 2.6939399130057105 1103 90-91 2.444410960005605 1103 92-93 2.2266685705877762 1103 94-95 2.508085412192056 1103 96-97 2.2536038131012575 1103 98-99 1.9295817542033227 1103 100-101 2.086702474826165 1104 1 0.021311699676349605 1104 2 -0.022143642151071674 1104 3 0.16966607296562586 1104 4 0.3062358055376393 1104 5 0.17448400629368876 1104 6 0.2635427247624236 1104 7 0.06752475833658877 1104 8 0.07830923574773152 1104 9 0.2584361456999389 1104 10-11 0.21586921010484872 1104 12-13 0.08917833315287993 1104 14-15 -0.05822050302234061 1104 16-17 0.2370164990745991 1104 18-19 0.2264754816753296 1104 20-21 0.22080369987383364 1104 22-23 0.27704985401970106 1104 24-25 0.1430646112056806 1104 26-27 -0.0022479410970177582 1104 28-29 0.0970220864005924 1104 30-31 0.42644738563987516 1104 32-33 0.047199030659818675 1104 34-35 0.27428997747067285 1104 36-37 0.49440896606157736 1104 38-39 0.42814945555812045 1104 40-41 0.4983839300800028 1104 42-43 0.3544306585936283 1104 44-45 0.3584901295534948 1104 46-47 0.17377185236428971 1104 48-49 0.28816662323837505 1104 50-51 0.597102968910967 1104 52-53 0.7668145355195506 1104 54-55 0.9517735208047213 1104 56-57 1.0229562161278665 1104 58-59 0.914589863559776 1104 60-61 0.9535445563147391 1104 62-63 0.8174415965812685 1104 64-65 0.8337883717017291 1104 66-67 0.8322087937918852 1104 68-69 0.6031740190619672 1104 70-71 0.5796743439024716 1104 72-73 0.415856177912989 1104 74-75 0.20905957415235932 1104 76-77 0.34143053148884306 1104 78-79 0.31617934621754884 1104 80-81 0.5417738069210998 1104 82-83 0.6225537543548789 1104 84-85 0.7409604424238445 1104 86-87 0.9285589417277222 1104 88-89 0.8967343996519794 1104 90-91 0.654293062229506 1104 92-93 0.8827244374056811 1104 94-95 0.9868053791944256 1104 96-97 0.8338762726848898 1104 98-99 0.7737218108034192 1104 100-101 0.9974656595555942 1105 1 -0.23149418417706613 1105 2 -0.1868982184140222 1105 3 -0.1305877927163479 1105 4 -0.217978607716816 1105 5 -0.19720146748770162 1105 6 -0.18305438189759826 1105 7 -0.31432141606548925 1105 8 -0.4374024991630492 1105 9 -0.34965718941491275 1105 10-11 -0.20742600288724589 1105 12-13 -0.09587486932017697 1105 14-15 -0.32306071340289577 1105 16-17 0.03105429955317618 1105 18-19 0.05008134609404635 1105 20-21 -0.12686170758666293 1105 22-23 -0.16086692905268762 1105 24-25 -0.30126562067450635 1105 26-27 -0.18601815014143597 1105 28-29 -0.321095602814367 1105 30-31 -0.22127298556276997 1105 32-33 -0.30659437854979643 1105 34-35 -0.3184590599046686 1105 36-37 -0.34931028764619754 1105 38-39 -0.16486680885088134 1105 40-41 -0.14826145964633497 1105 42-43 0.02602837258781676 1105 44-45 -0.42402198676314384 1105 46-47 0.07363763151328584 1105 48-49 0.09915815700008324 1105 50-51 -0.0790072379470459 1105 52-53 0.23594222145489852 1105 54-55 0.05009788583685548 1105 56-57 0.06689076979799324 1105 58-59 0.1256263400498696 1105 60-61 0.500262218806995 1105 62-63 0.299406808480164 1105 64-65 0.23835795397728532 1105 66-67 0.2855503463809512 1105 68-69 0.11418498751702799 1105 70-71 0.01641745369634151 1105 72-73 0.16068169080669392 1105 74-75 0.1304841979088387 1105 76-77 -0.0510272891532324 1105 78-79 0.07649036204468018 1105 80-81 0.2257262162157403 1105 82-83 0.23045122420073838 1105 84-85 0.23947702871995702 1105 86-87 0.3164253160642083 1105 88-89 0.5741670766595242 1105 90-91 0.2845085443921036 1105 92-93 0.14817046361822506 1105 94-95 0.22253909960206997 1105 96-97 0.21622046110424264 1105 98-99 0.3261529694755474 1105 100-101 0.16900264013305133 1106 1 -0.022197578176079702 1106 2 0.18399982107722934 1106 3 -0.020107151861875394 1106 4 -0.1724266436946138 1106 5 0.021897682255819007 1106 6 0.18489235410127236 1106 7 -0.1880653553529612 1106 8 0.14221242600032724 1106 9 -0.055334935181193146 1106 10-11 -0.021370911888723754 1106 12-13 -0.047064174358474986 1106 14-15 0.03631318864193389 1106 16-17 0.023842930007816676 1106 18-19 0.09033677438439724 1106 20-21 0.32754241018439956 1106 22-23 0.1987089967431146 1106 24-25 0.22378532444530919 1106 26-27 -0.13967565839806184 1106 28-29 -0.03921098472709872 1106 30-31 -0.01989922405620348 1106 32-33 0.24500073071870077 1106 34-35 0.15557649627550063 1106 36-37 -0.1402859745269538 1106 38-39 0.15314492609395813 1106 40-41 0.19161275860958682 1106 42-43 0.3335875743304939 1106 44-45 -0.03500329517435574 1106 46-47 -0.20266059067770215 1106 48-49 -0.18783854035076786 1106 50-51 -0.1281604949212607 1106 52-53 -0.14802587635972486 1106 54-55 0.07571098400283205 1106 56-57 0.09516531129190753 1106 58-59 -0.22533283358514922 1106 60-61 -0.08057363250352978 1106 62-63 -0.06086302498199103 1106 64-65 -0.6252935814032163 1106 66-67 -0.46278954540458983 1106 68-69 -0.18979927219767845 1106 70-71 -0.3395747464301415 1106 72-73 -0.1662630073873892 1106 74-75 0.057869834898511385 1106 76-77 0.20018485335256742 1106 78-79 -0.22997966817104754 1106 80-81 -0.14897513997848577 1106 82-83 -0.41278361523430007 1106 84-85 -0.40281796109102075 1106 86-87 -0.07976961050454534 1106 88-89 0.09415864436383004 1106 90-91 0.027613385935211454 1106 92-93 0.032955440078065834 1106 94-95 0.09156622348654864 1106 96-97 0.4300669933226402 1106 98-99 0.24324417880728788 1106 100-101 0.8535381611786548 1107 1 0.13461782286002588 1107 2 0.0034749987514537395 1107 3 0.046491395504901334 1107 4 -0.29394149543751524 1107 5 0.13290827762718038 1107 6 0.180863770028175 1107 7 0.214806068383794 1107 8 -0.15491354697756776 1107 9 0.1526675639727344 1107 10-11 0.15357311747633418 1107 12-13 0.2078169856654739 1107 14-15 -0.015355517264914909 1107 16-17 0.10772403796606511 1107 18-19 0.43869214886699837 1107 20-21 0.12151515378682376 1107 22-23 0.19725766516266674 1107 24-25 0.235906871838111 1107 26-27 0.22440030546833611 1107 28-29 0.21492498486653488 1107 30-31 0.036133238912071874 1107 32-33 0.12433845492665085 1107 34-35 0.032877149700127006 1107 36-37 0.0662001109128667 1107 38-39 -0.03924111509001449 1107 40-41 -0.1247547471827275 1107 42-43 -0.10177285052567697 1107 44-45 -0.18481585862944172 1107 46-47 0.01192171218851712 1107 48-49 -0.07379511755119239 1107 50-51 -0.22547455526541427 1107 52-53 -0.4067761692293246 1107 54-55 -0.22972597744252 1107 56-57 -0.1133090333310065 1107 58-59 -0.36924244751793367 1107 60-61 0.04716307121657337 1107 62-63 -0.0023346621881543683 1107 64-65 0.2821303375825437 1107 66-67 0.2811155127788325 1107 68-69 0.26577566337100933 1107 70-71 0.19746024172490806 1107 72-73 0.12759584248114564 1107 74-75 0.06277051956255875 1107 76-77 -0.15624070570295956 1107 78-79 -0.12352482215537464 1107 80-81 -0.5835968826176945 1107 82-83 -0.267598391481922 1107 84-85 -0.5246940186756923 1107 86-87 -0.7769391885119923 1107 88-89 -0.5997600810676644 1107 90-91 -0.40833603223051185 1107 92-93 -0.5630862645531778 1107 94-95 -0.6306925170440003 1107 96-97 -0.9560783220653803 1107 98-99 -0.8435330840984996 1107 100-101 -0.854614026882679 1108 1 0.005184671771740312 1108 2 0.1555102220419542 1108 3 0.20475428456121136 1108 4 0.18605878104043683 1108 5 0.30744880583829826 1108 6 0.2192665569258878 1108 7 0.08513099909150412 1108 8 0.17415741799958795 1108 9 0.26849800004757896 1108 10-11 -0.07919366646306258 1108 12-13 0.20898856096770402 1108 14-15 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-1.4799708869930583 2316 66-67 -0.9849064560023706 2316 68-69 -1.0642658565743304 2316 70-71 -1.1903991160298624 2316 72-73 -1.3507454619809316 2316 74-75 -2.0071275200653425 2316 76-77 -2.0485411209024136 2316 78-79 -2.5892813786277564 2316 80-81 -2.031707462320224 2316 82-83 -2.4209567769341724 2316 84-85 -2.517123198262592 2316 86-87 -2.36113533083606 2316 88-89 -2.038657437455953 2316 90-91 -2.721385908721814 2316 92-93 -2.4717791746283346 2316 94-95 -2.9108628013767905 2316 96-97 -3.1349215448540484 2316 98-99 -2.8292165864898386 2316 100-101 -3.4900873017026726 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 753.0 3 245.0 4 246.0 5 280.0 6 296.0 7 358.0 8 511.0 9 664.0 10 922.0 11 1454.0 12 2205.0 13 3015.0 14 3580.0 15 5113.0 16 7316.0 17 10524.0 18 9283.0 19 4021.0 20 3404.0 21 3301.0 22 3368.0 23 3643.0 24 3949.0 25 4377.0 26 5129.0 27 5808.0 28 6807.0 29 8226.0 30 9998.0 31 12256.0 32 15600.0 33 20602.0 34 28967.0 35 40635.0 36 57538.0 37 88197.0 38 93440.0 39 17006.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.529635198555205 20.887958280002817 15.37389352122244 19.208513000219536 2 15.053772348193817 22.541103280599913 41.49489820220391 20.910226169002357 3 18.19951418814959 29.858584436211828 31.96335495948462 19.978546416153968 4 11.511700406619424 19.272269300958445 40.357858236270125 28.85817205615201 5 11.094485398502442 40.79901516831462 33.73628810471712 14.370211328465823 6 25.605142545609826 40.33336995524526 19.363110402626827 14.698377096518087 7 24.825927437482 38.04342646897354 21.54812192226658 15.582524171277873 8 26.30831785927449 33.83099011870814 20.607716028914382 19.252975993102993 9 28.90567695361549 16.704722990025235 21.610051842243763 32.779548214115515 10-11 23.611819067155672 28.87445349246804 27.376038623319037 20.13768881705725 12-13 26.022858658400637 27.862615981998186 27.867692460703914 18.246832898897264 14-15 23.297670005096734 27.583277738873164 28.855078709324538 20.263973546705564 16-17 19.153780024236898 31.212984349591387 28.983707417112907 20.6495282090588 18-19 19.191581224116035 28.517681376804237 34.241002894239074 18.04973450484065 20-21 20.9105759101564 26.851983248585316 34.24261791092084 17.994822930337442 22-23 20.616218768639406 26.979090728345152 33.94463682152561 18.46005368148983 24-25 19.6581470872314 27.71056273946112 34.365006816974834 18.266283356332643 26-27 19.36526372123693 27.71233873580673 34.70356786837798 18.218829674578355 28-29 18.947401111812752 28.04552842161927 34.64435449642336 18.36271597014462 30-31 19.745449087229495 27.81778750847554 34.424413699580235 18.01234970471473 32-33 19.12705823407502 27.89477825005849 34.28464655206325 18.69351696380324 34-35 19.4138284073346 28.23052245503868 34.08064414278719 18.275004994839527 36-37 19.749276660058694 27.786398621125148 34.09588977282726 18.36843494598889 38-39 19.174810798388876 28.868164959168062 33.6757646995496 18.281259542893462 40-41 19.28074906444584 28.629872516187802 33.66017774235123 18.429200677015128 42-43 19.104774563266794 28.794061504954264 32.95736390776327 19.14380002401567 44-45 19.575798354122455 28.74737332436209 32.62833186688059 19.048496454634854 46-47 19.576009771649794 28.93578038631141 32.023994368879784 19.464215473159015 48-49 19.709525958620233 29.54436296937301 31.3609902326525 19.385120839354254 50-51 19.944992609093507 29.693225623465402 30.675781427914856 19.686000339526235 52-53 19.89602559815819 30.30060858772245 30.165619917868092 19.637745896251268 54-55 19.696914796367864 29.807036305830735 30.221247229362696 20.274801668438702 56-57 20.223380291021723 30.341851143299625 29.403732597911336 20.031035967767316 58-59 20.220966908792406 29.94026046891029 29.773909010824752 20.064863611472557 60-61 20.52995076869568 30.02519533110019 29.989172425500904 19.455681474703226 62-63 20.44990812391625 29.81019177514946 30.13193923238179 19.6079608685525 64-65 20.077076596290883 29.65399378087308 30.36213371536936 19.90679590746668 66-67 20.269199906815416 29.94579763414697 29.568400072476898 20.216602386560712 68-69 19.935062917508155 29.729894878566622 29.88535031847134 20.449691885453884 70-71 19.97022986398342 30.0385230117765 30.033136626693473 19.958110497546606 72-73 20.057243710179133 30.01971957828051 29.816623449218206 20.10641326232215 74-75 19.486307716358372 30.578656995638305 29.725781621250036 20.209253666753284 76-77 19.33568087998832 30.33605258062746 29.82291933060753 20.50534720877669 78-79 19.329500070928816 30.43063627806995 29.464347656812222 20.775515994189014 80-81 19.466145091429336 30.573791465904748 29.278935356647445 20.681128086018475 82-83 19.67198686207875 30.654804432598777 29.144378382072457 20.52883032325002 84-85 19.58423918669897 30.141830755533473 29.008634076650726 21.26529598111684 86-87 19.24003313658486 30.16951434192813 29.242311276794037 21.348141244692968 88-89 19.662570492007024 29.127789256733315 29.669457865328546 21.540182385931118 90-91 19.250418660720666 29.149660462789683 30.119648418202967 21.480272458286688 92-93 19.626089829158698 29.458810956129295 29.93072937648154 20.984369838230464 94-95 19.25742338503459 29.875643677518337 29.50315445982852 21.363778477618553 96-97 19.296436524920722 30.300978482901137 28.75638146649232 21.646203525685824 98-99 18.949354608871435 30.251362860598828 28.684590976123015 22.114691554406722 100-101 19.363126743724663 30.374297977616095 28.863078569526763 21.39949670913248 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 758.0 1 550.0 2 356.5 3 363.0 4 348.5 5 361.0 6 509.5 7 974.0 8 1692.5 9 1976.5 10 1589.0 11 1294.5 12 1292.5 13 1329.5 14 1386.5 15 1452.0 16 1546.5 17 1683.0 18 1774.5 19 1899.0 20 2115.0 21 2379.0 22 2703.0 23 3041.5 24 3342.5 25 3956.5 26 5388.0 27 6882.5 28 7320.5 29 7748.5 30 8892.5 31 10032.0 32 11735.5 33 13547.0 34 15835.5 35 17981.5 36 19669.5 37 21009.0 38 19554.5 39 17432.5 40 16437.5 41 15539.5 42 14891.0 43 15136.5 44 15661.5 45 15525.0 46 14686.0 47 14251.5 48 16154.5 49 16006.5 50 13566.0 51 12025.5 52 10520.0 53 9513.5 54 8495.5 55 7602.0 56 7131.0 57 6513.5 58 5631.0 59 4902.0 60 4436.5 61 4068.0 62 3407.0 63 2292.5 64 1421.5 65 1032.0 66 738.0 67 522.5 68 388.5 69 296.5 70 235.0 71 177.5 72 127.0 73 117.5 74 94.0 75 72.0 76 46.5 77 18.5 78 9.5 79 6.5 80 3.5 81 2.5 82 3.0 83 2.0 84 1.5 85 1.5 86 1.0 87 0.5 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.041611546893729076 2 0.03519384563151215 3 0.028155076505209722 4 0.05755228873859047 5 0.10765176310815483 6 0.13083829670068048 7 0.06997364602030064 8 0.1057885595158983 9 0.08715652359333304 10-11 0.08881270456422774 12-13 0.08715652359333304 14-15 0.07804752825341225 16-17 0.06293487689399821 18-19 0.0737000532048137 20-21 0.06904204422417237 22-23 0.039541320680110716 24-25 0.011075710242858238 26-27 0.004036941116555806 28-29 0.009108995339920795 30-31 0.0065212125728978405 32-33 0.008280904854473449 34-35 0.008384416165154366 36-37 0.007556325679707022 38-39 0.004658008980641315 40-41 0.007556325679707022 42-43 0.00434747504859856 44-45 0.002898316699065707 46-47 0.001863203592256526 48-49 2.0702262136183624E-4 50-51 0.0026912940777038706 52-53 0.007349303058345185 54-55 0.015009140048733124 56-57 0.010558153689453648 58-59 0.005279076844726824 60-61 0.0026912940777038706 62-63 0.010661665000134565 64-65 0.0026912940777038706 66-67 0.02618836160227228 68-69 0.054653972039524754 70-71 0.07049120257370523 72-73 0.003415873252470297 74-75 0.01749341150507516 76-77 0.025567293738186773 78-79 0.033227130728574715 80-81 0.08249851461269173 82-83 0.033537664660617465 84-85 0.0142845608739667 86-87 0.03912727543738704 88-89 0.056206641699738526 90-91 0.051652144029778135 92-93 0.046062533253008554 94-95 0.014905628738052209 96-97 0.02018470558277903 98-99 0.047822225534584165 100-101 0.08601789917584295 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 483039.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.30327079286792 #Duplication Level Percentage of deduplicated Percentage of total 1 83.36377272778725 43.60197979296554 2 9.301286941891433 9.729754592878278 3 2.568730912221812 4.030590854878442 4 1.184227660759397 2.47755920084413 5 0.6903593818421033 1.805402684644222 6 0.4208261024404173 1.320634895558899 7 0.30573325518721284 1.1193594465509247 8 0.2293699117897398 0.9597437286459991 9 0.18051516218949318 0.8497380069193979 >10 1.5068264983630508 15.795098334526115 >50 0.15551719912103787 5.58199742495154 >100 0.08728006072459987 8.017857346847663 >500 0.0035705479387336315 1.2070361331939146 >1k 0.001586910194992725 2.128342722738522 >5k 3.9672754874818127E-4 1.3749048338564342 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6626 1.3717318891435266 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4791 0.9918453789445572 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3040 0.629348768939982 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1361 0.28175778767345905 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1065 0.22047909175035557 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 857 0.17741838650709363 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 776 0.1606495541767849 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 722 0.14947033262324574 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 681 0.14098240514741045 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 653 0.13518577174927904 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 559 0.11572564534126643 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 538 0.11137817029266787 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 529 0.10951496670041136 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 502 0.10392535592364177 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 490 0.10144108446729974 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.035113106809181E-4 0.0 14-15 0.0 0.0 0.0 3.105339320427543E-4 0.0 16-17 0.0 0.0 0.0 4.140452427236724E-4 0.0 18-19 0.0 0.0 0.0 5.175565534045906E-4 0.0 20-21 0.0 0.0 0.0 0.0010351131068091811 0.0 22-23 0.0 0.0 0.0 0.0015526696602137717 0.0 24-25 0.0 0.0 0.0 0.0025877827670229524 0.0 26-27 0.0 0.0 0.0 0.0036228958738321335 0.0 28-29 0.0 0.0 0.0 0.007349303058345186 0.0 30-31 0.0 0.0 0.0 0.01418104956328578 0.0 32-33 0.0 0.0 0.0 0.018735547233246178 0.0 34-35 0.0 0.0 0.0 0.023600578835249325 0.0 36-37 0.0 0.0 0.0 0.030846370582913594 0.0 38-39 0.0 0.0 0.0 0.043474750485985605 0.0 40-41 0.0 0.0 0.0 0.06666128407851127 0.0 42-43 0.0 0.0 0.0 0.11220626077811523 0.0 44-45 0.0 0.0 0.0 0.14884926475916022 0.0 46-47 0.0 0.0 0.0 0.23880059374087806 0.0 48-49 0.0 0.0 0.0 0.3246114702953592 0.0 50-51 0.0 0.0 0.0 0.3835094060728016 0.0 52-53 0.0 0.0 0.0 0.4552427443746778 0.0 54-55 0.0 0.0 0.0 0.5095861824821598 0.0 56-57 0.0 0.0 0.0 0.6622653657365141 0.0 58-59 0.0 0.0 0.0 0.8573841863700447 0.0 60-61 0.0 0.0 0.0 1.0486730885083815 0.0 62-63 0.0 0.0 0.0 1.2848858994822363 0.0 64-65 0.0 0.0 0.0 1.5958338767677143 0.0 66-67 0.0 0.0 0.0 1.9913505948795025 0.0 68-69 0.0 0.0 0.0 2.428996416438424 0.0 70-71 0.0 0.0 0.0 2.7298002852771726 0.0 72-73 0.0 0.0 0.0 2.9591813497460864 0.0 74-75 0.0 0.0 0.0 3.2856560236337025 0.0 76-77 0.0 0.0 0.0 3.709431329561381 0.0 78-79 0.0 0.0 0.0 4.049673007769559 0.0 80-81 0.0 0.0 0.0 4.40047283966719 0.0 82-83 0.0 0.0 0.0 4.883456615304354 0.0 84-85 0.0 0.0 0.0 5.759472837596964 0.0 86-87 0.0 0.0 0.0 6.857934866542868 0.0 88-89 0.0 0.0 0.0 7.878349367235359 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3535 0.0 41.009228 1 GTATCAA 5780 0.0 36.840145 1 TATCAAC 7215 0.0 29.186602 2 ATCAACG 7290 0.0 28.821121 3 TCAACGC 7285 0.0 28.775654 4 CAACGCA 7360 0.0 28.482422 5 AACGCAG 7510 0.0 28.034315 6 GTGTAGC 190 3.9108272E-10 27.51758 1 GTAGTAC 70 0.008356502 27.163023 3 GTACATG 4595 0.0 26.89422 1 CATGGGA 1970 0.0 26.301252 4 TACATGG 4755 0.0 26.091925 2 ATATGAG 165 8.3289706E-8 25.928339 3 ACGCAGA 8340 0.0 25.184732 7 CGCAGAG 8360 0.0 25.181324 8 ACATGGG 4785 0.0 25.034258 3 CATTCCG 175 1.4777288E-7 24.436592 9 GAGTACT 4365 0.0 24.38877 12-13 GCAGAGT 8485 0.0 24.13579 9 ATGGGAT 910 0.0 22.984097 5 >>END_MODULE