FastQCFastQC Report
Thu 26 May 2016
SRR936880_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936880_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483039
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75431.5615716329323304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61501.2731891213752928No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39830.8245711008841937No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14680.30390920815917555No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14410.298319597382406No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13780.28527717223661025No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC10900.22565465728440148No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9900.20495239514821784No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA7650.1583723053418047No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA7070.1463649933028182No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC6920.14325965398239066No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA6290.13021722883659498No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT5980.12379952757437804No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5850.12110823349667418No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5830.12069418825395051No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5420.11220626077811523No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5040.10433940116636545No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA4940.1022691749527471No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36250.046.1270561
GTATCAA62500.038.3824271
CGGTATC851.6307022E-533.528062
ACGCGAA300.003951422431.6653978-79
GTAGTAC600.003949483431.665393
ATCAACG80950.029.572623
TCAACGC81300.029.4453074
TATCAAC81800.029.2653222
CAACGCA82400.029.0522255
AACGCAG84250.028.4172256
TAGGACA1554.5776687E-827.5795354
CGCGAAT350.0083925727.14176478-79
CTGCGAC458.948614E-426.38782528-29
ACGCAGA92250.025.8498737
CGCAGAG92950.025.65528
GTATAGG1301.0000478E-525.5785391
TAGATCA2251.0186341E-1025.3323125
GTAGGTA1701.1193333E-725.1486478
GCAGAGT95300.024.8730329
GAGTACT60650.024.8271612-13
Produced by FastQC (version 0.11.5)