##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936879_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 96569 Sequences flagged as poor quality 0 Sequence length 101 %GC 19 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.692354689393078 33.0 31.0 34.0 30.0 34.0 2 31.476177655355237 31.0 31.0 34.0 30.0 34.0 3 31.323292153796768 31.0 31.0 34.0 28.0 34.0 4 35.043699323799565 37.0 35.0 37.0 32.0 37.0 5 35.17355466039827 37.0 35.0 37.0 33.0 37.0 6 35.4401412461556 37.0 35.0 37.0 33.0 37.0 7 35.46032370636539 37.0 35.0 37.0 33.0 37.0 8 35.406414066625935 37.0 35.0 37.0 33.0 37.0 9 36.785024179602146 39.0 37.0 39.0 32.0 39.0 10-11 36.682620716793174 39.0 37.0 39.0 32.0 39.0 12-13 36.43230746927068 39.0 37.0 39.0 32.0 39.0 14-15 36.97914444594021 39.0 36.0 41.0 31.5 41.0 16-17 37.10213940291398 39.0 36.5 40.5 31.5 41.0 18-19 37.31408112334186 39.0 36.0 41.0 32.5 41.0 20-21 37.56306889374437 40.0 35.0 41.0 33.0 41.0 22-23 37.42684505379573 40.0 35.0 41.0 33.0 41.0 24-25 37.193007072663065 39.5 35.0 41.0 33.0 41.0 26-27 36.91401484948586 38.5 35.0 41.0 33.0 41.0 28-29 36.53652828547463 38.0 35.0 41.0 33.0 41.0 30-31 35.80519110687695 36.5 35.0 39.5 32.0 41.0 32-33 35.013570607544864 35.0 35.0 39.0 31.0 41.0 34-35 34.55576841429444 35.0 35.0 38.0 31.0 41.0 36-37 34.142877113773565 35.0 35.0 37.5 30.5 40.5 38-39 33.72772836003273 35.0 35.0 35.5 29.5 40.0 40-41 33.28340875436216 35.0 35.0 35.0 26.0 40.0 42-43 32.836852406051634 35.0 34.5 35.0 23.5 40.0 44-45 32.252632832482476 35.0 33.0 35.0 20.5 40.0 46-47 31.520487941264797 35.0 33.0 35.0 17.5 40.0 48-49 30.93207965289068 35.0 33.0 35.0 5.0 39.0 50-51 30.19496422247305 35.0 32.0 35.0 2.0 38.5 52-53 30.084147086539158 35.0 32.0 35.0 2.0 38.5 54-55 30.089169402189107 35.0 33.0 35.0 2.0 39.0 56-57 29.892807215566073 35.0 33.0 35.0 2.0 39.0 58-59 29.62921848626371 35.0 32.5 35.0 2.0 39.0 60-61 29.335889364081638 35.0 31.0 35.0 2.0 38.5 62-63 28.963326740465366 35.0 31.0 35.0 2.0 38.0 64-65 28.626510577928734 35.0 30.5 35.0 2.0 37.5 66-67 28.2401339974526 35.0 29.0 35.0 2.0 37.0 68-69 27.820920792386794 35.0 28.0 35.0 2.0 36.5 70-71 27.485212645880146 35.0 26.0 35.0 2.0 36.0 72-73 27.05732170779443 35.0 25.0 35.0 2.0 35.0 74-75 26.64878998436351 35.0 24.0 35.0 2.0 35.0 76-77 26.1698681771583 35.0 19.0 35.0 2.0 35.0 78-79 25.69394422640806 34.5 7.0 35.0 2.0 35.0 80-81 25.20829665834792 34.0 2.0 35.0 2.0 35.0 82-83 24.679912808458198 33.0 2.0 35.0 2.0 35.0 84-85 24.161190444138388 33.0 2.0 35.0 2.0 35.0 86-87 23.592534871439078 33.0 2.0 35.0 2.0 35.0 88-89 22.90389255351096 33.0 2.0 35.0 2.0 35.0 90-91 22.25811595853742 33.0 2.0 35.0 2.0 35.0 92-93 21.634768921703653 32.5 2.0 35.0 2.0 35.0 94-95 20.97468649359525 31.0 2.0 35.0 2.0 35.0 96-97 20.270573372407295 30.5 2.0 35.0 2.0 35.0 98-99 19.54491089272955 29.0 2.0 35.0 2.0 35.0 100-101 18.539287970259608 25.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9103279669895521 1101 2 0.8672941368097895 1101 3 1.0154366905050871 1101 4 0.8702064774257678 1101 5 0.9962906550336896 1101 6 0.8314710468251576 1101 7 0.7101858287878215 1101 8 0.6579326388097186 1101 9 0.8832574486756499 1101 10-11 1.0791772711407788 1101 12-13 1.253281148881527 1101 14-15 1.920141297327838 1101 16-17 1.6382242195521428 1101 18-19 1.3850045477184594 1101 20-21 1.3677370435769447 1101 22-23 1.2109502751250787 1101 24-25 1.2984789415515081 1101 26-27 1.290302289111061 1101 28-29 1.5036981777283955 1101 30-31 1.0214808046315156 1101 32-33 1.6656189783532724 1101 34-35 1.8936032145372863 1101 36-37 1.2286368350556103 1101 38-39 1.3407687454178117 1101 40-41 1.5162132221282079 1101 42-43 1.71593883896049 1101 44-45 1.5249686340461395 1101 46-47 1.3085606546447117 1101 48-49 1.0939631101716643 1101 50-51 1.577662260605166 1101 52-53 1.3234965006459802 1101 54-55 1.2375075024269542 1101 56-57 1.2326706728537467 1101 58-59 1.2922837048290603 1101 60-61 0.990969176847635 1101 62-63 0.2941198944954415 1101 64-65 0.16820916098130922 1101 66-67 0.3716076518523792 1101 68-69 0.6474392736345287 1101 70-71 0.24997708073502167 1101 72-73 -0.8403314647680915 1101 74-75 -0.8411317603132851 1101 76-77 -0.9237717960417591 1101 78-79 -0.7216527568208875 1101 80-81 -0.796192277646572 1101 82-83 -0.8170181498702931 1101 84-85 -0.4907003528717837 1101 86-87 -0.3747967723914414 1101 88-89 -0.1732410880609585 1101 90-91 0.11617977841408944 1101 92-93 -0.19193060897013225 1101 94-95 0.4441516542269177 1101 96-97 0.9079322379442836 1101 98-99 1.2186234304709345 1101 100-101 1.2925158527588287 1102 1 0.2777730219346033 1102 2 0.39751391702956695 1102 3 0.002387239955638165 1102 4 0.49383285105214725 1102 5 -0.35261044386741247 1102 6 0.1693831347372452 1102 7 0.25963637823836905 1102 8 0.05491066078774054 1102 9 -0.16825354033534268 1102 10-11 0.46550968872319487 1102 12-13 0.25671521481558557 1102 14-15 0.1206907478772834 1102 16-17 -0.10009995627203949 1102 18-19 -0.5233058918419786 1102 20-21 0.0991930875329885 1102 22-23 -0.2777173072925052 1102 24-25 -0.4271391903166304 1102 26-27 -0.11388727132850107 1102 28-29 0.25266795794817654 1102 30-31 0.12106871671942798 1102 32-33 1.1604678794521703 1102 34-35 1.149097720031797 1102 36-37 1.1390077141764863 1102 38-39 1.3929665476156075 1102 40-41 1.4619549803699599 1102 42-43 1.34093883896049 1102 44-45 2.066177425254928 1102 46-47 2.259453511787573 1102 48-49 2.372122450831 1102 50-51 1.9097364364293448 1102 52-53 1.5130569402064182 1102 54-55 2.031463546382998 1102 56-57 2.000184409117484 1102 58-59 1.8726408476862062 1102 60-61 1.9126724735509306 1102 62-63 1.6416473670229124 1102 64-65 1.379404215926364 1102 66-67 0.9509345749293026 1102 68-69 0.9510107022059557 1102 70-71 1.2067078499657917 1102 72-73 1.2791740297374012 1102 74-75 1.381739118807591 1102 76-77 1.2654452369252702 1102 78-79 1.470654935486806 1102 80-81 1.4139725575182673 1102 82-83 1.2681466852945427 1102 84-85 2.043640306468877 1102 86-87 1.673280150685482 1102 88-89 1.5637094613895925 1102 90-91 1.3963995586338704 1102 92-93 1.8867095009199772 1102 94-95 1.9640692366445016 1102 96-97 1.2369157544277982 1102 98-99 1.7890217821192884 1102 100-101 1.8206751934181646 1103 1 0.25505378572526993 1103 2 0.441361158896612 1103 3 0.5394275511296129 1103 4 -0.1527371630921266 1103 5 0.11910101299255871 1103 6 -0.13082902933630436 1103 7 0.21066183792719784 1103 8 -0.08264238023064507 1103 9 0.34650954311586446 1103 10-11 0.03300438462135702 1103 12-13 -0.3446815320019496 1103 14-15 0.06199202086173017 1103 16-17 -0.026903350897214295 1103 18-19 0.7577673047633908 1103 20-21 0.47012660945660656 1103 22-23 0.5620546163284246 1103 24-25 0.5915577686649911 1103 26-27 0.3018217102839458 1103 28-29 0.6297453978350163 1103 30-31 0.9497462273276298 1103 32-33 0.7655332967081776 1103 34-35 0.8832738162128422 1103 36-37 1.0963538952231602 1103 38-39 0.9026023326226849 1103 40-41 0.959568134542522 1103 42-43 0.6268299280694052 1103 44-45 0.04681745354347555 1103 46-47 -0.20381558297905045 1103 48-49 -0.3373011701025206 1103 50-51 -0.5591903669652787 1103 52-53 -0.616716751448461 1103 54-55 -0.5716128326834813 1103 56-57 -0.5277063263846422 1103 58-59 -0.3401420377451956 1103 60-61 -0.3007292237616568 1103 62-63 -0.07935687625855792 1103 64-65 0.26107854407346665 1103 66-67 0.3491400204738575 1103 68-69 0.3553600657137359 1103 70-71 0.3904949786786638 1103 72-73 0.05984941870487148 1103 74-75 -0.04643450212592981 1103 76-77 0.4393755763877891 1103 78-79 0.13278364835809242 1103 80-81 -0.6564835952398695 1103 82-83 -0.6030520417210141 1103 84-85 -0.4666142904193826 1103 86-87 -0.5074129186214478 1103 88-89 -0.10160171258494799 1103 90-91 0.1060883846593299 1103 92-93 0.47833786018447455 1103 94-95 0.7384681051027719 1103 96-97 0.4635777063372757 1103 98-99 0.665834016914193 1103 100-101 0.8878604833757358 1104 1 0.29465774612130957 1104 2 0.30274729751047147 1104 3 0.24239784815931387 1104 4 -0.0438262720030167 1104 5 0.1587049733886019 1104 6 0.21570562412904337 1104 7 0.12155292703610598 1104 8 0.22428831283865946 1104 9 0.5247273648980411 1104 10-11 0.3349845826411624 1104 12-13 0.4275956957208251 1104 14-15 0.5075365753171681 1104 16-17 0.4730966491027857 1104 18-19 0.47063859189211144 1104 20-21 0.29685928272393625 1104 22-23 0.4481932301898155 1104 24-25 0.012349847872904718 1104 26-27 0.361227650878007 1104 28-29 0.35251767506274234 1104 30-31 0.47944919762466753 1104 32-33 0.1615729006685811 1104 34-35 0.1258480736385863 1104 36-37 0.11615587542118533 1104 38-39 -0.5330412317337547 1104 40-41 -0.45627344961589245 1104 42-43 -0.412774032326638 1104 44-45 -0.7205092791297893 1104 46-47 -0.5503502364443982 1104 48-49 -1.614528892874798 1104 50-51 -1.1136458125098336 1104 52-53 -1.1563207118445007 1104 54-55 -1.2448801594161552 1104 56-57 -1.636617217473752 1104 58-59 -2.3153895624976712 1104 60-61 -1.8452836792071992 1104 62-63 -1.7823271732882624 1104 64-65 -1.7092184856295063 1104 66-67 -1.4874936428924777 1104 68-69 -1.753550825375374 1104 70-71 -1.4708911599351957 1104 72-73 -1.7470812743644366 1104 74-75 -1.4870285615318686 1104 76-77 -1.6695353147013172 1104 78-79 -1.5305826882755689 1104 80-81 -1.3000479516755128 1104 82-83 -0.8604777842952736 1104 84-85 -0.6002776567560204 1104 86-87 -0.9727594532749144 1104 88-89 -0.15605715812950294 1104 90-91 0.08133590941180557 1104 92-93 0.5872487512735844 1104 94-95 1.4315374120334674 1104 96-97 1.2606074093075748 1104 98-99 1.2697944129537966 1104 100-101 1.2690486021876133 1105 1 0.41240187347522195 1105 2 0.344292628514161 1105 3 0.619509088695132 1105 4 0.779897276822453 1105 5 0.48912625081045746 1105 6 0.4445932187708621 1105 7 -0.06074177302213002 1105 8 0.01219357395300591 1105 9 0.5616134064433709 1105 10-11 0.36895856827501916 1105 12-13 0.3503776798015821 1105 14-15 0.7670492912946543 1105 16-17 0.9818328168372048 1105 18-19 0.1833642762252481 1105 20-21 -0.2117312822149131 1105 22-23 0.18455510167108002 1105 24-25 -0.2498807869552806 1105 26-27 -0.13183194919965047 1105 28-29 -0.3183229384103754 1105 30-31 -0.3330629359416335 1105 32-33 -1.2863983670464236 1105 34-35 -1.0331501791278654 1105 36-37 -0.6217590925461991 1105 38-39 -0.20664829832276865 1105 40-41 0.6046370531990917 1105 42-43 -0.23994351398068403 1105 44-45 -0.7400200537366644 1105 46-47 0.3771881056251054 1105 48-49 1.162213487245566 1105 50-51 1.4203281731240232 1105 52-53 1.5214603015509596 1105 54-55 1.3602450589880384 1105 56-57 1.567323764859779 1105 58-59 1.4025778224761183 1105 60-61 1.4732467032428076 1105 62-63 1.9854849020369194 1105 64-65 2.026900714525805 1105 66-67 2.152527712184206 1105 68-69 2.0837137834384514 1105 70-71 2.499600006828537 1105 72-73 2.142794477916677 1105 74-75 1.8354330963986278 1105 76-77 1.8698745086339557 1105 78-79 2.2182039550946477 1105 80-81 2.7208703166219053 1105 82-83 2.7294562091040646 1105 84-85 2.806245348485678 1105 86-87 3.040927209509011 1105 88-89 2.963919545423206 1105 90-91 2.4461194465890514 1105 92-93 1.8158936745894465 1105 94-95 1.8443213374848355 1105 96-97 1.6227700961644942 1105 98-99 1.6037101574694255 1105 100-101 1.1610113278719503 1106 1 0.3859540850575307 1106 2 0.7380454784913582 1106 3 0.9635583363011513 1106 4 0.49923152214849154 1106 5 0.466326433208998 1106 6 0.7715426031757886 1106 7 0.6554004978397003 1106 8 0.33481099301365447 1106 9 0.6243129048473861 1106 10-11 0.7538194893876451 1106 12-13 0.4815906300979833 1106 14-15 0.3499266282759592 1106 16-17 0.6025578510369343 1106 18-19 0.06982118456998165 1106 20-21 0.4438774729150481 1106 22-23 0.510592493371945 1106 24-25 0.6427320721418468 1106 26-27 0.7551949545851215 1106 28-29 0.5546820775495007 1106 30-31 0.48693250409484534 1106 32-33 0.5615268495518393 1106 34-35 0.6522123379719957 1106 36-37 -8.066554407548665E-6 1106 38-39 0.32299146455913075 1106 40-41 0.3554350468151455 1106 42-43 0.2682644203558411 1106 44-45 0.556833571434332 1106 46-47 0.6399560034819167 1106 48-49 1.6480776003326625 1106 50-51 1.502863512841305 1106 52-53 0.768040328910736 1106 54-55 0.8325432806022626 1106 56-57 0.6886187944995434 1106 58-59 0.5335627745965041 1106 60-61 0.24946649348448702 1106 62-63 0.3252021842986572 1106 64-65 -0.34599030234605976 1106 66-67 -0.385547983210234 1106 68-69 -0.4397700286910542 1106 70-71 -0.02148982445281078 1106 72-73 -0.06114989052838027 1106 74-75 -0.17733480145818703 1106 76-77 0.06538294456646909 1106 78-79 0.4793758657193621 1106 80-81 -0.3291752830132957 1106 82-83 0.05697558894902954 1106 84-85 -0.460512517451388 1106 86-87 0.05700108091803813 1106 88-89 0.7501712554095263 1106 90-91 0.7950706549793836 1106 92-93 1.3567053480960567 1106 94-95 0.6459629243521405 1106 96-97 0.055852631504208716 1106 98-99 0.32338640005948704 1106 100-101 0.6329675522221514 1107 1 0.2729797888518988 1107 2 0.6140552704130258 1107 3 0.349849645970675 1107 4 0.5654493924055259 1107 5 0.33535948094461787 1107 6 0.12013501443649943 1107 7 0.5064408895165684 1107 8 0.6643091570283417 1107 9 0.29762991831128005 1107 10-11 0.4845416436104131 1107 12-13 0.7250422824847647 1107 14-15 0.9094125523885666 1107 16-17 0.7694489159084128 1107 18-19 0.9897110254512569 1107 20-21 0.9785163958036591 1107 22-23 0.9307883318052461 1107 24-25 0.9595619375029187 1107 26-27 1.0586221271677374 1107 28-29 0.9373014165947922 1107 30-31 1.3453168370201851 1107 32-33 0.9421878042642007 1107 34-35 0.9343420809340515 1107 36-37 0.4961975638005498 1107 38-39 0.9116695551344023 1107 40-41 0.4460715217233471 1107 42-43 0.8212019968552298 1107 44-45 0.5999180267586937 1107 46-47 1.0954027599078735 1107 48-49 -0.21838506796598978 1107 50-51 -0.44303611996163283 1107 52-53 -0.2647626086657624 1107 54-55 0.4515763283378824 1107 56-57 0.5916787700197368 1107 58-59 0.8330731785132741 1107 60-61 0.6027100675358952 1107 62-63 0.46082029935373825 1107 64-65 0.6696767723173451 1107 66-67 0.8578424696661457 1107 68-69 0.968491905213476 1107 70-71 0.2928920604921075 1107 72-73 0.5307717740983087 1107 74-75 0.45142896843165303 1107 76-77 -0.039207018713828035 1107 78-79 -0.3859240118816132 1107 80-81 -0.3927003748125628 1107 82-83 -0.5029999312468121 1107 84-85 0.15454256210797368 1107 86-87 0.08117488752758462 1107 88-89 0.3447244989835774 1107 90-91 0.16820406991206482 1107 92-93 0.4458730347545661 1107 94-95 -0.011870612979560491 1107 96-97 -0.035640636549644 1107 98-99 0.5818319324952235 1107 100-101 1.0808255693580158 1108 1 0.49267754810150777 1108 2 0.7581928420649291 1108 3 0.5097245808325823 1108 4 0.5106291735415383 1108 5 0.9804871516064182 1108 6 0.5919432478914217 1108 7 0.12155292703610598 1108 8 0.4520110851158847 1108 9 0.029677859947547347 1108 10-11 0.27062814699760196 1108 12-13 0.5711600521564648 1108 14-15 0.7501108327429193 1108 16-17 0.6018095203899136 1108 18-19 0.7280643344663673 1108 20-21 0.8909186886645273 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-0.35449523926520143 2316 78-79 0.22660731643918552 2316 80-81 -0.6282893472436406 2316 82-83 -0.4538771242292654 2316 84-85 -0.36826445543588804 2316 86-87 -0.5719579445526115 2316 88-89 -1.6803381576580279 2316 90-91 -1.4059813937470835 2316 92-93 -1.0147785943181162 2316 94-95 -2.040692668117405 2316 96-97 -1.9797509122388668 2316 98-99 -2.6136567893092852 2316 100-101 -2.8459914732484997 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 390.0 3 91.0 4 70.0 5 86.0 6 92.0 7 101.0 8 139.0 9 171.0 10 263.0 11 399.0 12 583.0 13 760.0 14 1026.0 15 1356.0 16 1869.0 17 2383.0 18 2118.0 19 1582.0 20 1524.0 21 1504.0 22 1541.0 23 1724.0 24 1819.0 25 1968.0 26 2127.0 27 2461.0 28 2657.0 29 3044.0 30 3534.0 31 4023.0 32 4722.0 33 5704.0 34 8337.0 35 18508.0 36 9951.0 37 3783.0 38 3319.0 39 839.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.40841596982602 21.775622493705118 16.487923155833258 6.3280383806356015 2 14.891874520536586 30.575770769836826 40.942547324334974 13.589807385291616 3 19.123514233308118 43.675063990300416 30.121555663789263 7.079866112602202 4 8.644292796249818 26.07081371471241 35.65680031528075 29.628093173757026 5 6.572945177075558 49.037903292693045 26.087542506427802 18.3016090238036 6 15.868095869961852 43.42448996516835 19.697503731962183 21.009910432907613 7 18.839227395726414 52.08650845489513 16.4100650057541 12.664199143624355 8 28.478132418339953 32.05448495340376 17.87657955570299 21.590803072553307 9 35.650442753157336 19.538168018083407 17.695609796561666 27.11577943219759 10-11 23.8683490233484 37.52902998382615 18.14633185418654 20.45628913863891 12-13 30.337125236454092 40.2674198647353 22.252856883729365 7.142598015081236 14-15 25.639577070591894 37.54794236550223 30.100549393593862 6.711931170312015 16-17 11.214793387144436 45.01936266504922 28.112930735055496 15.652913212750846 18-19 11.187125864883775 37.8667495918526 41.79843996993962 9.147684573324003 20-21 17.280547206964453 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46-47 5.7891355926935155 50.66723996808853 36.540713035009375 7.002911404208585 48-49 6.013604173466163 50.77682627998902 36.04691523035399 7.1626543161908325 50-51 6.45243164006858 50.96576694171212 35.58445863699698 6.997342781222321 52-53 5.609723243982551 51.12163380340065 35.683497217933706 7.585145734683094 54-55 5.363581730769231 50.652768567639264 35.740633289124666 8.243016412466844 56-57 5.879183504300073 50.628432286809655 35.0113977826132 8.480986426277068 58-59 5.739259289962902 49.739901763694014 35.579573480342376 8.941265466000704 60-61 5.863517903495728 49.207023579455665 35.99558565233386 8.93387286471475 62-63 6.689016933695359 49.01392832714988 36.02555599311874 8.271498746036023 64-65 6.35049730625777 48.80905511811024 36.74523414836303 8.09521342726896 66-67 5.879000165809982 48.74243491958216 36.92536478195987 8.453200132647986 68-69 5.786228692813844 49.06170664732397 36.87321900419667 8.27884565566551 70-71 5.81538684649409 48.49953903684598 36.61238696044004 9.072687156219896 72-73 5.745894125141783 47.936315563220894 36.56624041185641 9.751549899780915 74-75 6.053637465427764 47.53565990242083 36.980639547531 9.430063084620405 76-77 6.3552253960360146 46.95603974347019 37.24914265585014 9.439592204643645 78-79 6.169532622154985 46.70358991363276 37.3005963328895 9.82628113132276 80-81 6.0304370797807865 47.32665471836896 36.707346131134294 9.935562070715966 82-83 6.22101789054283 46.94700192221008 36.13962187899921 10.692358308247888 84-85 6.696373056994818 46.020725388601036 35.78082901554404 11.502072538860103 86-87 7.182051348480531 45.56646545247286 35.92217414958937 11.32930904945724 88-89 7.9526077649875875 44.52973157875642 36.15575584499049 11.361904811265504 90-91 7.662694300518134 44.39015544041451 36.26321243523316 11.683937823834196 92-93 7.861721587994175 44.324335528804546 35.806045405315764 12.007897477885507 94-95 8.278742899788547 43.83007172768357 35.4823997678179 12.40878560470998 96-97 8.572257810394511 43.06237950121276 35.04156560316769 13.323797085225033 98-99 9.327851343364062 42.48680485705694 34.90102346609704 13.284320333481961 100-101 10.238996319145627 42.206957333195085 34.88413085178081 12.669915495878481 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4327.0 1 2791.5 2 1419.5 3 1543.0 4 1296.5 5 1465.0 6 3015.5 7 5730.0 8 7739.5 9 7451.0 10 5319.0 11 3847.5 12 3771.5 13 3490.0 14 2717.0 15 1921.0 16 1391.0 17 1076.0 18 887.0 19 757.0 20 721.0 21 757.5 22 741.0 23 701.0 24 689.5 25 735.0 26 829.0 27 911.5 28 993.5 29 1109.0 30 1225.5 31 1290.5 32 1339.0 33 1407.5 34 1500.5 35 1519.5 36 1484.0 37 1455.0 38 1349.5 39 1214.0 40 1081.5 41 941.5 42 842.0 43 778.5 44 720.5 45 623.5 46 668.5 47 887.0 48 1952.5 49 1951.0 50 964.5 51 724.5 52 405.5 53 278.5 54 239.5 55 216.0 56 202.5 57 191.0 58 172.5 59 161.5 60 144.5 61 136.0 62 131.0 63 84.0 64 40.0 65 29.5 66 31.0 67 24.5 68 19.0 69 19.0 70 21.0 71 13.0 72 10.5 73 25.0 74 28.0 75 16.5 76 7.0 77 4.5 78 6.0 79 4.0 80 2.0 81 1.0 82 0.5 83 0.0 84 0.0 85 1.5 86 2.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06420279799935798 2 0.11080160299889198 3 0.07248702999927513 4 0.15222276299847778 5 0.11701477699882984 6 0.10873054499891269 7 0.12012136399879877 8 0.10562395799894377 9 0.1315121829986849 10-11 0.12115689299878843 12-13 0.09475090349905249 14-15 0.10251737099897483 16-17 0.12478124449875219 18-19 0.09992854849900071 20-21 0.08180679099918194 22-23 0.08284231999917158 24-25 0.07559361699924406 26-27 0.061096210999389035 28-29 0.07818243949921817 30-31 0.05643633049943564 32-33 0.05384750799946153 34-35 0.051776449999482235 36-37 0.05695409499943046 38-39 0.05384750799946153 40-41 0.07921796849920781 42-43 0.07559361699924406 44-45 0.06834491399931655 46-47 0.05384750799946153 48-49 0.05643633049943564 50-51 0.04090339549959097 52-53 0.06006068199939939 54-55 0.059025152999409754 56-57 0.061096210999389035 58-59 0.0693804429993062 60-61 0.06782714949932173 62-63 0.07766467499922336 64-65 0.05074092099949259 66-67 0.07559361699924406 68-69 0.06627385599933726 70-71 0.03313692799966863 72-73 0.03158363449968416 74-75 0.03313692799966863 76-77 0.051776449999482235 78-79 0.06472056249935279 80-81 0.043492217999565076 82-83 0.06782714949932173 84-85 0.07145150099928549 86-87 0.0740403234992596 88-89 0.10096407749899036 90-91 0.07145150099928549 92-93 0.08543114249914568 94-95 0.09630419699903696 96-97 0.09837525499901624 98-99 0.1366898279986331 100-101 0.12840559599871595 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 96569.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.541747351634584 #Duplication Level Percentage of deduplicated Percentage of total 1 82.13840399002493 30.01480806469985 2 9.374291543867603 6.85105986393149 3 3.3240761732033555 3.64402655096356 4 1.3914078440262978 2.033778955979662 5 0.7764679211063251 1.4186747299858133 6 0.45624574926320566 1.000321013989997 7 0.35706189072772615 0.9133365779908666 8 0.3400589435502154 0.9941078399900589 9 0.18986624348220357 0.6244239869937557 >10 1.3659034232600318 9.88308877590117 >50 0.133189752890501 3.4358852219656413 >100 0.12468827930174563 8.154790874918453 >500 0.011335298118340512 3.0423842019695764 >1k 0.011335298118340512 13.737327713862626 >5k 0.005667649059170256 14.251985626857481 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6924 7.1700027959283 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6839 7.081982830929181 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4792 4.962254967950377 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3781 3.915335148960847 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3490 3.61399620996386 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1203 1.2457413869875427 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 884 0.915407635990846 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 771 0.7983928589920161 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 717 0.7424742929925753 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 566 0.586109413994139 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 462 0.4784143979952159 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 364 0.37693255599623066 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 334 0.34586668599654136 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 290 0.30030340999699695 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 283 0.2930547069970695 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 280 0.2899481199971005 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 278 0.28787706199712126 No Hit GTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 271 0.28062835899719374 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 254 0.2630243659973698 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 230 0.23817166999761827 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 208 0.21539003199784612 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 205 0.21228344499787719 No Hit GAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 192 0.1988215679980118 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 191 0.19778603899802213 No Hit TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 177 0.1832886329981671 No Hit GTACATGGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 172 0.17811098799821887 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 168 0.1739688719982603 No Hit GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 168 0.1739688719982603 No Hit GTACATGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 164 0.16982675599830171 No Hit GTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 162 0.16775569799832246 No Hit ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 158 0.16361358199836387 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 157 0.16257805299837422 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 152 0.157400407998426 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 150 0.15532934999844672 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 145 0.15015170499849848 No Hit GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 139 0.14393853099856063 No Hit GAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 139 0.14393853099856063 No Hit GAGTACATGGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 138 0.14290300199857098 No Hit GGTATCAACGCAGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 136 0.14083194399859167 No Hit GAGTACATGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 130 0.13461876999865383 No Hit GTATCAACGCAGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 129 0.13358324099866417 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 127 0.1315121829986849 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 125 0.12944112499870558 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 118 0.12219242199877807 No Hit GTACATGGGGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAAA 116 0.12012136399879877 No Hit GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 114 0.11805030599881951 No Hit GTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 110 0.11390818999886092 No Hit GAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 110 0.11390818999886092 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 109 0.11287266099887128 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 106 0.10976607399890234 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 105 0.10873054499891269 No Hit GTATCAACGCAGAGTACATGGGATGAAAAAAAAAAAAAAAAAAAAAAAAA 104 0.10769501599892306 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 104 0.10769501599892306 No Hit ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 101 0.10458842899895412 No Hit GTATCAACGCAGAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAA 100 0.10355289999896447 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 100 0.10355289999896447 No Hit TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 100 0.10355289999896447 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 99 0.10251737099897483 No Hit GTACATGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 97 0.10044631299899554 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.177644999948223E-4 0.0 14-15 0.0 0.0 0.0 0.0010355289999896447 0.0 16-17 0.0 0.0 0.0 0.0015532934999844669 0.0 18-19 0.0 0.0 0.0 0.0020710579999792893 0.0 20-21 0.0 0.0 0.0 0.0046598804999534015 0.0 22-23 0.0 0.0 0.0 0.009319760999906803 0.0 24-25 0.0 0.0 0.0 0.018121757499818782 0.0 26-27 0.0 0.0 0.0 0.03520798599964792 0.0 28-29 0.0 0.0 0.0 0.04815209849951848 0.0 30-31 0.0 0.0 0.0 0.07714691049922853 0.0 32-33 0.0 0.0 0.0 0.09526866799904732 0.0 34-35 0.0 0.0 0.0 0.12219242199877808 0.0 36-37 0.0 0.0 0.0 0.14549182449854509 0.0 38-39 0.0 0.0 0.0 0.16775569799832243 0.0 40-41 0.0 0.0 0.0 0.18587745549814122 0.0 42-43 0.0 0.0 0.0 0.23506508299764933 0.0 44-45 0.0 0.0 0.0 0.2852882394971471 0.0 46-47 0.0 0.0 0.0 0.32567387049674323 0.0 48-49 0.0 0.0 0.0 0.3815924364961841 0.0 50-51 0.0 0.0 0.0 0.4307800639956922 0.0 52-53 0.0 0.0 0.0 0.5094802679949052 0.0 54-55 0.0 0.0 0.0 0.5441704894945583 0.0 56-57 0.0 0.0 0.0 0.629601631993704 0.0 58-59 0.0 0.0 0.0 0.7450631154925493 0.0 60-61 0.0 0.0 0.0 0.8258343774917416 0.0 62-63 0.0 0.0 0.0 0.9345649224906543 0.0 64-65 0.0 0.0 0.0 1.023102651989769 0.0 66-67 0.0 0.0 0.0 1.136493077488635 0.0 68-69 0.0 0.0 0.0 1.2571322059874288 0.0 70-71 0.0 0.0 0.0 1.3456699354865433 0.0 72-73 0.0 0.0 0.0 1.4901262309850987 0.0 74-75 0.0 0.0 0.0 1.61697853348383 0.0 76-77 0.0 0.0 0.0 1.69101885698309 0.0 78-79 0.0 0.0 0.0 1.7676480029823236 0.0 80-81 0.0 0.0 0.0 1.8867338379811327 0.0 82-83 0.0 0.0 0.0 1.9840735639801592 0.0 84-85 0.0 0.0 0.0 2.150275968478497 0.0 86-87 0.0 0.0 0.0 2.4055338669759445 0.0 88-89 0.0 0.0 0.0 2.629725895473703 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAACCCA 25 0.004984455 57.066044 5 AACCCAG 25 0.004984455 57.066044 6 ATCAACC 25 0.004984455 57.066044 3 GGGTCTG 45 1.4952413E-5 52.83893 8 GGTCTGT 45 1.4952413E-5 52.83893 9 GGGGTCT 45 1.4952413E-5 52.83893 7 TGGGGTC 55 8.047409E-7 51.87822 6 TATGGGG 60 1.4662273E-6 47.555035 4 TAATGGG 130 0.0 43.874176 2 AATGGGA 110 3.274181E-11 43.23185 3 ATATGGG 110 3.274181E-11 43.23185 3 TATATGG 115 5.2750693E-11 41.330746 2 GTATATG 115 5.2750693E-11 41.309307 1 ATGGGGT 140 0.0 40.76146 5 GTAATGG 140 0.0 40.719173 1 GGTATCA 2190 0.0 40.13039 1 ATGGGTT 85 3.5621088E-7 39.162968 5 TGGGGTG 50 0.0015993485 38.04403 6 TATGGGA 50 0.0015993485 38.04403 4 GTATCAA 3605 0.0 36.765858 1 >>END_MODULE