##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936879_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 96569 Sequences flagged as poor quality 0 Sequence length 101 %GC 18 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.827387670991726 31.0 31.0 34.0 30.0 34.0 2 31.63914920937361 31.0 31.0 34.0 30.0 34.0 3 31.68097422568319 31.0 31.0 34.0 30.0 34.0 4 35.64557984446355 37.0 35.0 37.0 33.0 37.0 5 35.6496184075635 37.0 35.0 37.0 33.0 37.0 6 35.855460862181445 37.0 35.0 37.0 35.0 37.0 7 35.75566693245244 37.0 35.0 37.0 33.0 37.0 8 35.800660667501994 37.0 35.0 37.0 35.0 37.0 9 37.25862336774741 39.0 37.0 39.0 34.0 39.0 10-11 37.22726237198273 39.0 37.0 39.0 33.5 39.0 12-13 36.99675361658503 39.0 37.0 39.0 32.5 39.0 14-15 37.58586606467914 39.5 36.5 41.0 32.0 41.0 16-17 37.6099783574439 39.0 36.5 40.5 32.5 41.0 18-19 37.73310793318767 39.0 35.5 41.0 33.0 41.0 20-21 37.89319553894107 40.0 35.0 41.0 33.0 41.0 22-23 37.6397394609036 40.0 35.0 41.0 33.0 41.0 24-25 37.37875508703621 39.0 35.0 41.0 33.0 41.0 26-27 36.9795068810902 38.5 35.0 41.0 33.0 41.0 28-29 36.507704335759925 37.5 35.0 40.5 33.0 41.0 30-31 35.495811285195046 36.0 35.0 39.0 32.0 41.0 32-33 34.37808717083122 35.0 33.5 37.5 30.0 41.0 34-35 33.8901562613261 35.0 33.0 36.0 29.0 40.0 36-37 33.33910985927161 35.0 33.0 35.0 24.5 40.0 38-39 32.891310876161086 35.0 33.0 35.0 22.5 40.0 40-41 32.38927606167611 35.0 33.0 35.0 21.5 40.0 42-43 31.829463906636704 35.0 33.0 35.0 20.5 40.0 44-45 31.006492766829936 35.0 33.0 35.0 15.5 40.0 46-47 30.106405782393935 35.0 31.0 35.0 4.5 40.0 48-49 29.386055566486142 35.0 28.0 35.0 2.0 39.0 50-51 29.12314510867877 35.0 27.0 35.0 2.0 39.0 52-53 28.906579751265934 35.0 27.0 35.0 2.0 39.0 54-55 28.659295426068407 35.0 27.0 35.0 2.0 38.0 56-57 28.341382845426587 35.0 26.5 35.0 2.0 38.0 58-59 28.01398481914486 35.0 25.5 35.0 2.0 38.0 60-61 27.671157410763286 34.5 26.0 35.0 2.0 38.0 62-63 27.232455549917674 33.5 24.0 35.0 2.0 37.0 64-65 26.815292692271846 33.0 22.0 35.0 2.0 36.5 66-67 26.419632594310805 33.0 22.0 35.0 2.0 36.0 68-69 25.97527156748025 33.0 20.0 35.0 2.0 36.0 70-71 25.502832171814973 33.0 17.5 35.0 2.0 35.0 72-73 25.36291149333637 33.0 14.0 35.0 2.0 35.0 74-75 25.0298128799097 33.0 4.5 35.0 2.0 35.0 76-77 23.648432726858516 30.5 2.0 34.0 2.0 35.0 78-79 24.259431080367406 33.0 2.0 35.0 2.0 35.0 80-81 23.92719195601073 33.0 2.0 35.0 2.0 35.0 82-83 23.43794074703062 33.0 2.0 35.0 2.0 35.0 84-85 22.897767399476024 33.0 2.0 35.0 2.0 35.0 86-87 22.27339001128727 32.5 2.0 35.0 2.0 35.0 88-89 21.618656090463816 31.0 2.0 35.0 2.0 35.0 90-91 20.822769211651774 30.5 2.0 35.0 2.0 35.0 92-93 20.014782176474853 29.0 2.0 35.0 2.0 35.0 94-95 19.207457879857927 28.0 2.0 34.5 2.0 35.0 96-97 18.296689413787036 25.0 2.0 34.0 2.0 35.0 98-99 17.39570669676604 19.0 2.0 33.5 2.0 35.0 100-101 15.258763164162414 2.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4083101285390178 1101 2 0.5463298335086222 1101 3 0.7576383791295598 1101 4 0.6893195521446316 1101 5 0.610656017152742 1101 6 0.5854586060525477 1101 7 0.7507401836411418 1101 8 0.7202215352044732 1101 9 0.6384032647871649 1101 10-11 0.43471286096055195 1101 12-13 0.5623435641741139 1101 14-15 0.9500502700247111 1101 16-17 1.0702367984918126 1101 18-19 0.7285438176659795 1101 20-21 0.9303855612196159 1101 22-23 1.1884611692902851 1101 24-25 1.170115771224296 1101 26-27 1.1614850919151891 1101 28-29 0.7259142759564199 1101 30-31 0.5202372217166911 1101 32-33 1.2035207756369957 1101 34-35 1.0022303266468668 1101 36-37 1.2262781537598073 1101 38-39 1.6157683263919864 1101 40-41 0.753497715899762 1101 42-43 -0.2496308152107467 1101 44-45 -0.6899516662619334 1101 46-47 -0.7085592089039459 1101 48-49 -0.31639668344403304 1101 50-51 0.06604377561584585 1101 52-53 -0.3415509698984174 1101 54-55 -0.3264285455649265 1101 56-57 -0.16405337754834548 1101 58-59 0.02926844165112996 1101 60-61 -0.296971784999581 1101 62-63 -0.2153296152735642 1101 64-65 0.20224993144269376 1101 66-67 -0.4195759087083637 1101 68-69 -0.09653153300780914 1101 70-71 -0.23842979481550586 1101 72-73 0.10788822468164838 1101 74-75 -0.6813873704900502 1101 76-77 -0.7964748283456977 1101 78-79 -1.3763649068176527 1101 80-81 -1.2994833952537483 1101 82-83 -0.8975621654028707 1101 84-85 -0.9583245580473054 1101 86-87 -1.2213961967046139 1101 88-89 -0.6323611405753411 1101 90-91 0.009126775344139304 1101 92-93 0.25747851188904747 1101 94-95 1.1258125766535727 1101 96-97 1.1571944349549739 1101 98-99 1.335258997649749 1101 100-101 0.7133989294348364 1102 1 -0.029189871460982175 1102 2 0.16652214120092879 1102 3 0.4224735439647205 1102 4 0.4963250466501208 1102 5 0.2892274457241726 1102 6 0.24205201264595644 1102 7 -0.07068838778742759 1102 8 0.18175999674293308 1102 9 0.14321095709485832 1102 10-11 0.18883374008142795 1102 12-13 0.2598023553829094 1102 14-15 -0.0475458838214422 1102 16-17 0.12449504025005353 1102 18-19 0.5437910704132278 1102 20-21 0.754217978802032 1102 22-23 0.7767166637957743 1102 24-25 0.9458712657297852 1102 26-27 0.6597680589481598 1102 28-29 0.07103790233005469 1102 30-31 0.07209161732109237 1102 32-33 0.5713092371754627 1102 34-35 0.6419968101633486 1102 36-37 1.1627479339795883 1102 38-39 1.6689963483700083 1102 40-41 1.1333054082074554 1102 42-43 0.906962591382662 1102 44-45 1.3509137183534534 1102 46-47 1.3697374943927585 1102 48-49 1.1939055143581676 1102 50-51 1.1306042151762838 1102 52-53 1.3050836454861958 1102 54-55 1.0599038720174896 1102 56-57 1.1264686004736326 1102 58-59 0.9159442658269548 1102 60-61 0.6899787644509701 1102 62-63 1.3952473078033591 1102 64-65 1.7242279534207157 1102 66-67 1.7459460693136144 1102 68-69 1.487259675783399 1102 70-71 0.9597158095800964 1102 72-73 1.0000585543519769 1102 74-75 0.3608516404989608 1102 76-77 0.18566802879715638 1102 78-79 0.20055817010542398 1102 80-81 0.31624462672427356 1102 82-83 0.28581695547624975 1102 84-85 0.9668128045900559 1102 86-87 0.3067631439547256 1102 88-89 1.1849465517323523 1102 90-91 2.046214687432048 1102 92-93 2.0627669734275074 1102 94-95 0.9300708184118172 1102 96-97 1.0593235558340979 1102 98-99 1.7834046020453513 1102 100-101 1.0973275008634058 1103 1 0.1286071582419872 1103 2 0.11639837882469095 1103 3 0.1272118749406772 1103 4 0.2534855841324415 1103 5 0.27154710626165013 1103 6 0.21217223895430237 1103 7 0.5081659262153977 1103 8 0.33027484822807907 1103 9 0.6562060065998097 1103 10-11 0.7671576439003829 1103 12-13 0.20733594802788957 1103 14-15 -0.016295883821442203 1103 16-17 0.5606690147903706 1103 18-19 0.5838370392958296 1103 20-21 0.47022752618534014 1103 22-23 0.4258945280107653 1103 24-25 0.5288362586576483 1103 26-27 0.25007039275296705 1103 28-29 0.208016332315907 1103 30-31 0.25362450275248705 1103 32-33 0.830282009452695 1103 34-35 0.3859412938974387 1103 36-37 0.1493550768367271 1103 38-39 0.6010596439852804 1103 40-41 0.45199352701933293 1103 42-43 0.9451521246217034 1103 44-45 0.9230053025118679 1103 46-47 -0.048048927106531636 1103 48-49 -0.1536015577776162 1103 50-51 0.18771171164021894 1103 52-53 -0.14324556243459696 1103 54-55 -0.6727251944605861 1103 56-57 -0.49526053672579806 1103 58-59 -0.6234828911744579 1103 60-61 -0.9641937956621831 1103 62-63 -0.2743071476421868 1103 64-65 -0.19364686977588974 1103 66-67 -0.13754049362839282 1103 68-69 -0.6493209465645506 1103 70-71 -0.3270150956532838 1103 72-73 0.28144115689794447 1103 74-75 0.10302809028679505 1103 76-77 -0.313182749137777 1103 78-79 -0.07976361207279581 1103 80-81 0.41600594214152764 1103 82-83 0.46925047739987136 1103 84-85 0.5282519842223046 1103 86-87 0.44201774084298506 1103 88-89 1.2078425913363127 1103 90-91 1.6323179406145094 1103 92-93 1.266665488278992 1103 94-95 1.5562200404768802 1103 96-97 1.7876209391438564 1103 98-99 1.7417232017624649 1103 100-101 1.6577058601279067 1104 1 0.019696267152880864 1104 2 -0.2697402350366964 1104 3 -0.2490257488216976 1104 4 0.015861821756203653 1104 5 -0.0749875472036905 1104 6 -0.05515449371896608 1104 7 0.12202731235401387 1104 8 0.2510669274359998 1104 9 -0.19527914191504436 1104 10-11 0.09389031716771257 1104 12-13 -0.1936541509820131 1104 14-15 -0.7440186560986675 1104 16-17 -0.7165587079819105 1104 18-19 -0.5993312775358532 1104 20-21 -0.6634358401512941 1104 22-23 -0.004798541296160863 1104 24-25 -0.550371662134431 1104 26-27 -0.36379099338564913 1104 28-29 -0.7226767369910263 1104 30-31 -0.7810289625940499 1104 32-33 -0.9221932380720617 1104 34-35 -1.1982171219441469 1104 36-37 -0.9249023489058459 1104 38-39 -0.6761680787869935 1104 40-41 -0.5925609284262094 1104 42-43 -1.0449468852792911 1104 44-45 -1.1364006380821898 1104 46-47 -0.8648806102748523 1104 48-49 -0.23775997361920176 1104 50-51 -0.015258585389485546 1104 52-53 -0.8016614040187555 1104 54-55 -0.16282420436157352 1104 56-57 -0.49526053672579806 1104 58-59 -1.1333838812734705 1104 60-61 -0.9146888451671344 1104 62-63 -0.6703467516025796 1104 64-65 -0.9411716222511401 1104 66-67 -1.5137781173907676 1104 68-69 -1.5255585703269254 1104 70-71 -0.7725596501087288 1104 72-73 -0.8076677539931438 1104 74-75 -1.3128134938716194 1104 76-77 -0.8428857194348076 1104 78-79 -0.7827339091024967 1104 80-81 -0.6186475232050057 1104 82-83 -0.6248089285407197 1104 84-85 -0.5064014811242288 1104 86-87 -0.9787743383649321 1104 88-89 -0.925820775000318 1104 90-91 -0.8924345346330149 1104 92-93 -0.5006612443942728 1104 94-95 0.6502794464174713 1104 96-97 0.8618783648864294 1104 98-99 -0.25827679823753513 1104 100-101 -0.4215020606641726 1105 1 0.05046699128411447 1105 2 0.030492729436222277 1105 3 0.28591892211598235 1105 4 0.19572980855488709 1105 5 0.06653837009391594 1105 6 0.29982512188965416 1105 7 0.19191665422937376 1105 8 0.18175999674293308 1105 9 0.50963252572231 1105 10-11 0.19338556080972324 1105 12-13 -0.024949045177315554 1105 14-15 0.4837041161785578 1105 16-17 0.8384306144797478 1105 18-19 0.24249555220594488 1105 20-21 0.36354921129503026 1105 22-23 -0.0698344566524014 1105 24-25 0.41672210606592586 1105 26-27 -0.06598474217229011 1105 28-29 0.15200779028523215 1105 30-31 0.2216889562566564 1105 32-33 0.4800102175676173 1105 34-35 0.6035689390148846 1105 36-37 0.2866099787975145 1105 38-39 0.816842987025467 1105 40-41 1.02178580036432 1105 42-43 0.256402367293024 1105 44-45 1.3012813654122795 1105 46-47 1.3238691470538235 1105 48-49 1.467539968139679 1105 50-51 2.126752674560038 1105 52-53 2.0556963905842345 1105 54-55 2.283205692745785 1105 56-57 2.0601170598573866 1105 58-59 1.8777789997205119 1105 60-61 2.406365319072819 1105 62-63 2.6869139744700234 1105 64-65 2.741559886193823 1105 66-67 3.0068004110503104 1105 68-69 3.156202252814211 1105 70-71 2.7439420000562897 1105 72-73 2.8531748008505744 1105 74-75 2.846233293160026 1105 76-77 2.4817114461641054 1105 78-79 3.064528758340721 1105 80-81 3.5339442065562032 1105 82-83 3.463863173963645 1105 84-85 3.5720298914247905 1105 86-87 3.144648298016353 1105 88-89 3.1297994929088233 1105 90-91 2.8322791132023575 1105 92-93 2.5266150126431945 1105 94-95 2.6060161965630755 1105 96-97 2.403423695890119 1105 98-99 2.013709223894093 1105 100-101 1.4462420666897398 1106 1 0.460635709934369 1106 2 0.07495237375906783 1106 3 0.11765626821721398 1106 4 0.09495461475643197 1106 5 0.09321415668762256 1106 6 0.01323141463931421 1106 7 0.22748436968765162 1106 8 0.22827162464990636 1106 9 0.1243156082576462 1106 10-11 0.11449819190867316 1106 12-13 0.11310385039951854 1106 14-15 -0.19071448847260797 1106 16-17 0.09584055520021906 1106 18-19 0.16235419333681023 1106 20-21 0.2750859190013699 1106 22-23 0.303398557483483 1106 24-25 -0.5172724219778573 1106 26-27 -0.3829022068325756 1106 28-29 -0.09787821062676016 1106 30-31 0.013640620643343482 1106 32-33 0.04441970229174075 1106 34-35 -0.25324820644794954 1106 36-37 -0.25180771386094847 1106 38-39 0.33189086664243206 1106 40-41 -0.8012876150483592 1106 42-43 -0.5689510298797948 1106 44-45 -0.5742316304837587 1106 46-47 -0.33914954879660897 1106 48-49 -1.576235681655124 1106 50-51 -1.7684821635612593 1106 52-53 -1.2290733312579896 1106 54-55 -1.2898677226668944 1106 56-57 -0.9979084759383277 1106 58-59 -0.6052351361663995 1106 60-61 -0.8357687438546755 1106 62-63 -0.7762643201036177 1106 64-65 -0.48860427249290694 1106 66-67 -0.9670937977960214 1106 68-69 -0.7940941448146077 1106 70-71 -0.38008900769564846 1106 72-73 -0.4424248343523409 1106 74-75 -0.16562261199273465 1106 76-77 -0.28630040974104887 1106 78-79 -0.15845345780155284 1106 80-81 -0.18219806430562713 1106 82-83 -0.056998659141687114 1106 84-85 0.3133659607362347 1106 86-87 0.337078758572666 1106 88-89 1.032330272662584 1106 90-91 1.0051017306214156 1106 92-93 0.9210518571484378 1106 94-95 1.0307767984782643 1106 96-97 1.3611300342394088 1106 98-99 1.5598788545370468 1106 100-101 1.6581663712952999 1107 1 0.34778381274954384 1107 2 0.05928529909566649 1107 3 0.23112016050607664 1107 4 0.35187015943207456 1107 5 0.10802443820537633 1107 6 0.06855577204445495 1107 7 0.3244243941674583 1107 8 -0.10245052957285594 1107 9 0.3688688518316994 1107 10-11 0.38451043181075306 1107 12-13 0.40374032530772297 1107 14-15 0.4310725372311879 1107 16-17 0.1923521831071966 1107 18-19 -0.159310433346171 1107 20-21 -0.16885532946864146 1107 22-23 0.3141696713145734 1107 24-25 -0.10154414780404153 1107 26-27 0.038762419850414176 1107 28-29 0.12672399255561828 1107 30-31 0.4277777075466531 1107 32-33 0.2078223950702025 1107 34-35 0.2576453064039512 1107 36-37 -0.14538176526853874 1107 38-39 -0.015355155389386255 1107 40-41 0.2918580397863977 1107 42-43 0.3663610876232646 1107 44-45 0.3492689815113472 1107 46-47 -0.8439843101185183 1107 48-49 -1.5497974931606286 1107 50-51 -1.6037942810643173 1107 52-53 -1.6353768808295932 1107 54-55 -1.8572483836216058 1107 56-57 -1.7993772641880206 1107 58-59 -1.6631910725189094 1107 60-61 -1.509269355849785 1107 62-63 -1.7350158500913793 1107 64-65 -1.3288735503386846 1107 66-67 -1.4884524269269868 1107 68-69 -1.4596388204572008 1107 70-71 -1.9788650174875713 1107 72-73 -1.44909558098637 1107 74-75 -1.2637254271702112 1107 76-77 -1.2971327230825374 1107 78-79 -1.6935207772629965 1107 80-81 -1.5542910875614417 1107 82-83 -0.7907807888846499 1107 84-85 -0.6274541127031767 1107 86-87 -0.8686127958948973 1107 88-89 -1.4472902903729121 1107 90-91 -1.8874319291093045 1107 92-93 -1.4500619739409117 1107 94-95 -1.0645720387310433 1107 96-97 -1.3110241885268081 1107 98-99 -0.9630709618644211 1107 100-101 0.05155682417167817 1108 1 0.22761705923208453 1108 2 -0.06181944295748565 1108 3 0.28562771652483576 1108 4 -0.12275203962993686 1108 5 0.05372532408343744 1108 6 0.04385540727113124 1108 7 0.17153226284906253 1108 8 0.36987880862412226 1108 9 0.2898693729364439 1108 10-11 0.19785071320730907 1108 12-13 0.4400092153546211 1108 14-15 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0.33785491387863686 2316 70-71 0.6487039048181913 2316 72-73 0.8839871257805463 2316 74-75 0.19091116430848132 2316 76-77 -0.18635578072665027 2316 78-79 0.48567721772447214 2316 80-81 0.6647565314861801 2316 82-83 1.062602669761965 2316 84-85 -0.3683062430289894 2316 86-87 -0.5801416179500336 2316 88-89 -0.7192201149343163 2316 90-91 -1.0240234078060446 2316 92-93 -1.0327687408582094 2316 94-95 -1.1550482292072317 2316 96-97 -0.7147835870230494 2316 98-99 -0.9252263503356026 2316 100-101 -0.5735058324699249 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 9.0 5 33.0 6 71.0 7 114.0 8 187.0 9 304.0 10 432.0 11 592.0 12 763.0 13 1005.0 14 1242.0 15 1759.0 16 2284.0 17 2992.0 18 2565.0 19 1755.0 20 1587.0 21 1652.0 22 1803.0 23 1884.0 24 2094.0 25 2336.0 26 2528.0 27 2847.0 28 3311.0 29 3592.0 30 4029.0 31 4532.0 32 5390.0 33 6250.0 34 9361.0 35 15541.0 36 4732.0 37 3666.0 38 2972.0 39 355.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.166746069556936 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5.384288791318035 44-45 5.247052610323242 35.31586386865282 53.31966427975996 6.117419241263973 46-47 5.2325280369476745 35.09511333864905 52.182377367478175 7.4899812569251 48-49 5.568557197444314 35.154656256148456 51.59471465998406 7.682071886423179 50-51 6.024189957439758 35.55385268564446 50.88123517899119 7.540722177924593 52-53 5.2589340264474105 35.78322235914217 50.87864635649121 8.079197257919208 54-55 5.0737056846836115 35.573930918072 50.42587232632435 8.92649107092004 56-57 5.615673766943844 35.63514171214365 49.59925027700401 9.1499342439085 58-59 5.396687325577181 34.99122384628528 50.07688842635021 9.535200401787332 60-61 5.649357709812206 34.686258976788494 50.15714233937567 9.50724097402362 62-63 6.394909339436052 34.635856227153646 50.04400998249956 8.925224450910747 64-65 6.143275792438567 34.475349232155246 50.71244395199288 8.668931023413311 66-67 5.717673373442824 34.629125288653704 50.78182439499218 8.871376942911287 68-69 5.624475763443755 34.831571208151686 50.57368306599427 8.970269962410297 70-71 5.578423606041307 34.42737538638376 50.25500033654867 9.739200671026266 72-73 5.618780353943812 33.873189118661266 50.07145150099929 10.436579026395634 74-75 5.829601056255987 33.64641313071168 50.286587102285964 10.237398710746369 76-77 5.721327347944723 33.19094735861073 50.42016806722689 10.66755722621766 78-79 5.659713053428395 33.08998275835288 50.39635077690966 10.853953411309071 80-81 5.369874645969358 33.770510477235895 49.52649210647585 11.3331227703189 82-83 5.388430950211251 33.557182503520835 48.959282578079694 12.09510396818822 84-85 5.722969544461359 32.73789182640032 48.50414737953959 13.034991249598724 86-87 6.004577085577003 32.348189876563666 48.44099494656615 13.206238091293182 88-89 6.661592548294734 31.36219367599165 48.69703888949295 13.279174886220662 90-91 6.283589971937165 31.18495583468815 48.68591369901314 13.845540494361543 92-93 6.081661816939183 31.504416531184955 48.0402613675196 14.373660284356262 94-95 6.350899356936492 31.324752249686753 47.3495635245265 14.974784868850252 96-97 6.508851229955938 30.546192599035916 47.04846818579558 15.89648798521257 98-99 6.888963678256142 30.096564579180367 46.77142931110363 16.24304243145986 100-101 7.11201317192888 29.797346974702027 47.02699624102973 16.063643612339362 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6896.0 1 4492.5 2 2287.0 3 2307.0 4 1637.0 5 1519.5 6 2979.5 7 5838.5 8 7992.0 9 7585.0 10 5185.5 11 3198.0 12 2738.0 13 2491.0 14 2024.5 15 1458.5 16 1105.0 17 914.0 18 758.0 19 676.5 20 645.5 21 624.5 22 622.0 23 648.0 24 646.5 25 673.0 26 740.5 27 836.5 28 951.5 29 1040.5 30 1106.0 31 1162.5 32 1252.0 33 1341.0 34 1393.0 35 1441.5 36 1486.5 37 1482.5 38 1401.0 39 1316.0 40 1210.0 41 1046.5 42 932.5 43 871.5 44 797.0 45 704.5 46 711.0 47 881.5 48 2005.5 49 1983.0 50 949.0 51 737.5 52 424.5 53 263.0 54 196.5 55 177.0 56 190.0 57 184.0 58 146.5 59 133.0 60 114.5 61 102.0 62 92.0 63 55.5 64 25.5 65 16.0 66 22.0 67 24.0 68 16.0 69 16.0 70 16.5 71 12.0 72 8.0 73 10.5 74 13.0 75 8.0 76 7.0 77 8.0 78 6.5 79 3.5 80 1.0 81 0.0 82 0.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01553293499984467 2 0.0020710579999792893 3 0.003106586999968934 4 0.0 5 0.0010355289999896447 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.001553293499984467 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0025888224999741118 22-23 0.0 24-25 0.004659880499953401 26-27 0.0025888224999741118 28-29 0.0020710579999792893 30-31 0.019157286499808426 32-33 0.0 34-35 0.0 36-37 0.004659880499953401 38-39 0.0036243514999637566 40-41 0.0051776449999482235 42-43 0.0010355289999896447 44-45 5.177644999948223E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0025888224999741118 56-57 0.0 58-59 5.177644999948223E-4 60-61 5.177644999948223E-4 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 5.177644999948223E-4 72-73 0.0 74-75 0.001553293499984467 76-77 5.177644999948223E-4 78-79 5.177644999948223E-4 80-81 0.0025888224999741118 82-83 0.0010355289999896447 84-85 0.0020710579999792893 86-87 0.0010355289999896447 88-89 5.177644999948223E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 5.177644999948223E-4 98-99 0.001553293499984467 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 96569.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.559496318694407 #Duplication Level Percentage of deduplicated Percentage of total 1 82.54549151163972 25.225486439747748 2 9.179627935346142 5.6104961219438945 3 3.0666531123987664 2.8114612349718855 4 1.3113754193351632 1.60299889198397 5 0.7149876317305411 1.0924830949890751 6 0.5726678187794382 1.0500264059894997 7 0.39646233607807263 0.8480982509915189 8 0.30158246077733725 0.7372966479926271 9 0.17281691572633934 0.4753078109952469 >10 1.4469180983362135 8.42402841491576 >50 0.14570837992612926 3.0672368979693276 >100 0.10843414320084035 6.627385599933726 >500 0.010165700925078784 2.6292081309737076 >1k 0.010165700925078784 5.515227453944848 >5k 0.016942834875131308 34.28325860265717 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8229 8.521368140914786 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7580 7.8493098199215074 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5946 6.157255433938428 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5850 6.057844649939422 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5502 5.697480557943025 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2182 2.259524277977405 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1755 1.8173533949818264 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1389 1.4383497809856165 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 992 1.0272447679897276 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 851 0.8812351789911876 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 696 0.7207281839927927 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 450 0.46598804999534016 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 438 0.45356170199546436 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 431 0.4463129989955369 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 423 0.4380287669956197 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 390 0.40385630999596145 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 353 0.36554173699634457 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 298 0.30858764199691413 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 269 0.27855730099721443 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 219 0.22678085099773218 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 203 0.21021238699789785 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 190 0.19675050999803248 No Hit GTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 173 0.17914651699820855 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 163 0.1687912269983121 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 161 0.1667201689983328 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 160 0.16568463999834315 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 159 0.1646491109983535 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 158 0.16361358199836387 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 141 0.14600958899853989 No Hit GAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 130 0.13461876999865383 No Hit GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 129 0.13358324099866417 No Hit TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 128 0.13254771199867452 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 121 0.125299008998747 No Hit ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 117 0.12115689299878843 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 117 0.12115689299878843 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 116 0.12012136399879877 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 115 0.11908583499880913 No Hit GTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 112 0.11597924799884021 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 111 0.11494371899885057 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 111 0.11494371899885057 No Hit GTACATGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 106 0.10976607399890234 No Hit CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 105 0.10873054499891269 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 103 0.10665948699893339 No Hit GGTATCAACGCAGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 97 0.10044631299899554 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.177644999948223E-4 0.0 14-15 0.0 0.0 0.0 0.0010355289999896447 0.0 16-17 0.0 0.0 0.0 0.0015532934999844669 0.0 18-19 0.0 0.0 0.0 0.003106586999968934 0.0 20-21 0.0 0.0 0.0 0.0056954094999430455 0.0 22-23 0.0 0.0 0.0 0.010355289999896447 0.0 24-25 0.0 0.0 0.0 0.019157286499808426 0.0 26-27 0.0 0.0 0.0 0.036243514999637565 0.0 28-29 0.0 0.0 0.0 0.0486698629995133 0.0 30-31 0.0 0.0 0.0 0.07921796849920781 0.0 32-33 0.0 0.0 0.0 0.0973397259990266 0.0 34-35 0.0 0.0 0.0 0.12529900899874702 0.0 36-37 0.0 0.0 0.0 0.15118723399848813 0.0 38-39 0.0 0.0 0.0 0.17293334299827068 0.0 40-41 0.0 0.0 0.0 0.1920906294980791 0.0 42-43 0.0 0.0 0.0 0.2428315504975717 0.0 44-45 0.0 0.0 0.0 0.2935724714970643 0.0 46-47 0.0 0.0 0.0 0.33395810249666047 0.0 48-49 0.0 0.0 0.0 0.39298325549607016 0.0 50-51 0.0 0.0 0.0 0.44475970549555244 0.0 52-53 0.0 0.0 0.0 0.5239776739947601 0.0 54-55 0.0 0.0 0.0 0.568505420994315 0.0 56-57 0.0 0.0 0.0 0.6627385599933726 0.0 58-59 0.0 0.0 0.0 0.7890730979921092 0.0 60-61 0.0 0.0 0.0 0.8796818854912032 0.0 62-63 0.0 0.0 0.0 0.9972144269900278 0.0 64-65 0.0 0.0 0.0 1.0898942724891012 0.0 66-67 0.0 0.0 0.0 1.2100156364878998 0.0 68-69 0.0 0.0 0.0 1.3446344064865536 0.0 70-71 0.0 0.0 0.0 1.4409386034855907 0.0 72-73 0.0 0.0 0.0 1.5900547794840993 0.0 74-75 0.0 0.0 0.0 1.7262268429827377 0.0 76-77 0.0 0.0 0.0 1.8064803404819352 0.0 78-79 0.0 0.0 0.0 1.8841450154811585 0.0 80-81 0.0 0.0 0.0 2.0099617889799 0.0 82-83 0.0 0.0 0.0 2.115067982478849 0.0 84-85 0.0 0.0 0.0 2.2895546189771045 0.0 86-87 0.0 0.0 0.0 2.556721100974433 0.0 88-89 0.0 0.0 0.0 2.7881618324721185 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATGGG 70 0.0 74.62089 3 TATGGGA 45 2.395609E-9 73.867134 4 TATATGG 75 0.0 69.646164 2 GTATATG 70 0.0 67.83717 1 TGGGTGT 40 7.4834934E-6 59.388275 6 GGTATCA 2000 0.0 43.449703 1 TGGGGTG 55 4.9229428E-5 43.191475 8 ATGGGTG 155 0.0 42.8906 5 ATGGGTT 70 4.277874E-6 40.7023 5 CATGGGT 235 0.0 38.39295 4 GTATCAA 3340 0.0 38.102554 1 ATGGGGT 120 3.7089194E-9 35.632965 7 TGGGAGA 215 0.0 35.356743 6 ATGGGAG 370 0.0 32.085148 5 TGGGTGA 105 1.8746377E-6 31.673748 6 CATCTCG 30 0.0039485726 31.657345 50-51 GGGAGAG 190 1.0913936E-11 30.006708 7 GGAGAGT 160 1.8553692E-9 29.694138 8 TATCAAC 4410 0.0 28.857716 2 GAGAGTA 170 3.5452103E-9 27.947424 9 >>END_MODULE