##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936878_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 85673 Sequences flagged as poor quality 0 Sequence length 101 %GC 19 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.736287978709747 33.0 31.0 34.0 30.0 34.0 2 31.582190421719794 33.0 31.0 34.0 30.0 34.0 3 31.41035098572479 31.0 31.0 34.0 28.0 34.0 4 35.18075706465281 37.0 35.0 37.0 33.0 37.0 5 35.25821437325645 37.0 35.0 37.0 33.0 37.0 6 35.5260116956334 37.0 35.0 37.0 33.0 37.0 7 35.540777140989576 37.0 35.0 37.0 33.0 37.0 8 35.488835455744514 37.0 35.0 37.0 33.0 37.0 9 36.916881631319086 39.0 37.0 39.0 33.0 39.0 10-11 36.74155218096716 39.0 37.0 39.0 32.0 39.0 12-13 36.55796458627572 39.0 37.0 39.0 32.0 39.0 14-15 37.12009034351546 39.0 36.5 41.0 31.5 41.0 16-17 37.22204195020602 39.0 36.5 40.5 32.0 41.0 18-19 37.44378625704715 39.0 36.0 41.0 33.0 41.0 20-21 37.669779277018435 40.0 36.0 41.0 33.0 41.0 22-23 37.56086515004728 40.0 35.0 41.0 33.0 41.0 24-25 37.33968111306946 40.0 35.0 41.0 33.0 41.0 26-27 37.000268462642836 39.0 35.0 41.0 33.0 41.0 28-29 36.63327419373665 38.0 35.0 41.0 33.0 41.0 30-31 35.86923534835946 36.5 35.0 39.5 33.0 41.0 32-33 35.01334142612025 35.0 35.0 38.5 31.5 41.0 34-35 34.58882611791346 35.0 35.0 38.0 31.0 41.0 36-37 34.1287861986857 35.0 35.0 37.0 31.0 40.5 38-39 33.70643610005486 35.0 35.0 35.5 30.0 40.0 40-41 33.23408191612293 35.0 35.0 35.0 26.0 40.0 42-43 32.74142962193457 35.0 34.5 35.0 23.0 40.0 44-45 32.126696858987074 35.0 33.0 35.0 20.5 40.0 46-47 31.288095432633384 35.0 33.0 35.0 11.5 40.0 48-49 30.625634680704536 35.0 33.0 35.0 2.0 39.0 50-51 29.470422420132365 34.0 29.5 35.0 2.0 38.5 52-53 29.59989728385839 35.0 31.0 35.0 2.0 38.5 54-55 29.69167065469868 35.0 33.0 35.0 2.0 39.0 56-57 29.466045311825198 35.0 32.5 35.0 2.0 39.0 58-59 29.147689470428254 35.0 31.0 35.0 2.0 39.0 60-61 28.81703687276038 35.0 31.0 35.0 2.0 38.5 62-63 28.426073558764138 35.0 29.0 35.0 2.0 38.0 64-65 28.065242258354438 35.0 28.0 35.0 2.0 37.5 66-67 27.648109672825747 35.0 27.0 35.0 2.0 37.0 68-69 27.234624677553022 35.0 26.0 35.0 2.0 36.0 70-71 26.81138164882752 35.0 24.0 35.0 2.0 36.0 72-73 26.365803695446637 35.0 20.0 35.0 2.0 35.0 74-75 25.911944253148597 35.0 7.5 35.0 2.0 35.0 76-77 25.421223722759795 35.0 2.0 35.0 2.0 35.0 78-79 24.897517304168176 34.0 2.0 35.0 2.0 35.0 80-81 24.370980355537917 33.0 2.0 35.0 2.0 35.0 82-83 23.797847629941757 33.0 2.0 35.0 2.0 35.0 84-85 23.253673852905816 33.0 2.0 35.0 2.0 35.0 86-87 22.6544944148098 33.0 2.0 35.0 2.0 35.0 88-89 22.002848038471864 33.0 2.0 35.0 2.0 35.0 90-91 21.322546193083 31.5 2.0 35.0 2.0 35.0 92-93 20.69167065469868 31.0 2.0 35.0 2.0 35.0 94-95 20.016989016376222 30.0 2.0 35.0 2.0 35.0 96-97 19.277246040176017 28.0 2.0 35.0 2.0 35.0 98-99 18.59042522148168 26.0 2.0 35.0 2.0 35.0 100-101 17.587209505912014 22.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4854964733030691 1101 2 0.5368087094136378 1101 3 0.46558792921584313 1101 4 0.6106829792061248 1101 5 0.5261143478885089 1101 6 0.47058302381735473 1101 7 0.6326501288944044 1101 8 0.6198359783815519 1101 9 0.08147504957337048 1101 10-11 0.3905005421821315 1101 12-13 0.28756064285349936 1101 14-15 0.3845755401222135 1101 16-17 0.5259853435822492 1101 18-19 0.5219840004210994 1101 20-21 0.4675541503986409 1101 22-23 0.5719998865788796 1101 24-25 0.030715021833266576 1101 26-27 -0.1267968417121068 1101 28-29 0.10145503607239448 1101 30-31 -0.07679466066856122 1101 32-33 0.3150243967800108 1101 34-35 -0.06446615780379261 1101 36-37 -0.3130275815834338 1101 38-39 -0.0033652219733539823 1101 40-41 0.1992241155690735 1101 42-43 0.08373956631199775 1101 44-45 0.40825772983244235 1101 46-47 -0.4861914446608253 1101 48-49 -0.3521385817957743 1101 50-51 0.45016267001096466 1101 52-53 0.8120514173950504 1101 54-55 0.344303087631161 1101 56-57 0.09174841925173283 1101 58-59 0.32973096839237925 1101 60-61 0.4120287538273786 1101 62-63 0.7104908177247715 1101 64-65 0.9039202490056049 1101 66-67 1.3199011058106258 1101 68-69 1.6246899722404429 1101 70-71 1.2349107697814468 1101 72-73 1.8875508684357136 1101 74-75 1.9443031507746085 1101 76-77 2.1694879462209506 1101 78-79 1.7779339282791078 1101 80-81 1.1049066925596378 1101 82-83 1.0584161517746828 1101 84-85 1.1275956087878214 1101 86-87 0.9583386264529956 1101 88-89 1.0879042193763944 1101 90-91 1.4354113987211292 1101 92-93 1.4618548644473073 1101 94-95 1.3432148346301673 1101 96-97 1.7191732020146837 1101 98-99 1.9231122461643935 1101 100-101 1.5805004288538704 1102 1 0.4202790819987179 1102 2 0.19577610071798546 1102 3 0.5380516973317846 1102 4 0.5590525444235155 1102 5 0.08499115948271196 1102 6 0.04848157454199509 1102 7 0.3138095491842634 1102 8 0.27835047113516964 1102 9 0.2793917162400348 1102 10-11 0.36536467261691286 1102 12-13 0.4669084689404528 1102 14-15 0.8696298879482995 1102 16-17 0.8357679522779051 1102 18-19 0.3464858120153025 1102 20-21 0.28820632431168747 1102 22-23 0.4175614807817709 1102 24-25 0.21595052907964174 1102 26-27 0.2701687379980413 1102 28-29 0.7567992389709488 1102 30-31 0.9236582378821652 1102 32-33 1.1703233098234946 1102 34-35 0.7942294943701214 1102 36-37 0.4057224184165662 1102 38-39 0.6859463722295374 1102 40-41 0.274858173540089 1102 42-43 0.2986399286308412 1102 44-45 1.1901870776585284 1102 46-47 0.9870875408464244 1102 48-49 1.2592744616824838 1102 50-51 1.9112133946486445 1102 52-53 1.9200677217428783 1102 54-55 1.813053087631161 1102 56-57 1.5251723322952095 1102 58-59 1.6159628524503482 1102 60-61 1.4600360002041874 1102 62-63 1.7967679916378145 1102 64-65 0.4530597417592226 1102 66-67 0.4978902362454072 1102 68-69 1.0286754794868216 1102 70-71 1.0496752625350645 1102 72-73 1.2924421727835416 1102 74-75 1.9447560493253313 1102 76-77 1.9801763520180522 1102 78-79 2.077979218134182 1102 80-81 2.2414556056031145 1102 82-83 2.732782093803671 1102 84-85 2.801055753715353 1102 86-87 2.5772244960182142 1102 88-89 3.129344436767699 1102 90-91 3.03618132625736 1102 92-93 2.9349074006791938 1102 94-95 3.473423167963503 1102 96-97 2.528729361434973 1102 98-99 1.9640995650049753 1102 100-101 2.1416417332016984 1103 1 0.5903351829804855 1103 2 0.25152645134912177 1103 3 0.6026847034093912 1103 4 0.26054857060397296 1103 5 0.9085068210067888 1103 6 0.7622496904840261 1103 7 0.777811419216988 1103 8 0.45249726870412843 1103 9 0.6207895657024025 1103 10-11 0.40142124110686694 1103 12-13 0.48782946005780303 1103 14-15 0.9554760777566216 1103 16-17 1.071684268313433 1103 18-19 0.574571366012492 1103 20-21 0.3963175912588497 1103 22-23 0.7087606392670409 1103 24-25 0.5216424411880993 1103 26-27 0.8962206314061802 1103 28-29 0.8605276167175546 1103 30-31 1.1090252318045586 1103 32-33 1.0772891817262504 1103 34-35 1.2473617991854553 1103 36-37 0.9326041388466777 1103 38-39 1.7849412296395428 1103 40-41 1.4798020725583214 1103 42-43 1.2391495125485577 1103 44-45 0.8970010094023309 1103 46-47 1.0060800607155187 1103 48-49 0.6794474397096018 1103 50-51 0.277447616247521 1103 52-53 0.05885921309397801 1103 54-55 -0.23533400914303115 1103 56-57 -0.11490480655471913 1103 58-59 -0.2845163434355804 1103 60-61 -0.2902561924091849 1103 62-63 -0.2859809564687765 1103 64-65 -0.3633848585212789 1103 66-67 -0.2980424425764703 1103 68-69 -0.21536379120041715 1103 70-71 0.0356500170932712 1103 72-73 0.028007857683025605 1103 74-75 -0.13869416105334764 1103 76-77 0.531719129016647 1103 78-79 0.8325374229027638 1103 80-81 1.31777631621555 1103 82-83 1.5950424958607066 1103 84-85 1.3718832431964216 1103 86-87 0.8019206157003076 1103 88-89 1.0057469613118784 1103 90-91 0.8623267213017733 1103 92-93 1.3007325526193512 1103 94-95 1.485519942157051 1103 96-97 1.9031449762082318 1103 98-99 2.0234146655192333 1103 100-101 1.7629601062732227 1104 1 -0.08634260715670194 1104 2 -0.22863669288521393 1104 3 -0.4510787374508247 1104 4 -0.45469058401226903 1104 5 0.13171779616437362 1104 6 -0.05384226353896793 1104 7 -0.4650510205308791 1104 8 -0.23864103311270668 1104 9 -0.8786542607714622 1104 10-11 -0.3503399750592493 1104 12-13 -1.0242209663419075 1104 14-15 -1.416789402406522 1104 16-17 -1.1320606334292478 1104 18-19 -0.9358105398087915 1104 20-21 -0.9448021714404433 1104 22-23 -0.8170087341107788 1104 24-25 -0.5386096908104108 1104 26-27 -1.1391890830914093 1104 28-29 -0.9125535846172568 1104 30-31 -0.8199699480248768 1104 32-33 -1.1887471549441244 1104 34-35 -1.7541213302175862 1104 36-37 -1.697725857445505 1104 38-39 -1.3029341874906066 1104 40-41 -1.7228304821320712 1104 42-43 -1.4925894566765052 1104 44-45 -1.2772666954549123 1104 46-47 -0.8845391458102476 1104 48-49 0.05375222280192915 1104 50-51 -0.06348675527639358 1104 52-53 -1.298760364214143 1104 54-55 -0.7961423146676907 1104 56-57 -0.8180935347712541 1104 58-59 -0.9044644339064725 1104 60-61 -0.7366781427243474 1104 62-63 -0.47934395239017036 1104 64-65 -1.0050237165116407 1104 66-67 -1.3341937217755806 1104 68-69 -0.9715743955756508 1104 70-71 -1.3006495750461404 1104 72-73 -1.449374418920609 1104 74-75 -1.1494468492253915 1104 76-77 -1.628644237687098 1104 78-79 -1.569946818847324 1104 80-81 -0.7411780775553041 1104 82-83 -0.4792993654666944 1104 84-85 -0.08325209236160447 1104 86-87 -0.36511683331712064 1104 88-89 -0.22154261970406353 1104 90-91 -0.25298658978461575 1104 92-93 -0.3594454229090118 1104 94-95 0.15014730589453507 1104 96-97 0.4355884318997383 1104 98-99 0.22753034961267105 1104 100-101 -0.24816336424957797 1105 1 0.04726117918542272 1105 2 0.46291165059011163 1105 3 0.5018624390197637 1105 4 0.16288886155906823 1105 5 0.4013594459277243 1105 6 0.17009282773892664 1105 7 0.40715993281597207 1105 8 0.43870852740115396 1105 9 0.15880348094592023 1105 10-11 0.6303902480644865 1105 12-13 0.5066782899123226 1105 14-15 0.6533255401222107 1105 16-17 0.6759853435822478 1105 18-19 0.4937364514014888 1105 20-21 0.3954953268692307 1105 22-23 0.7755538081475066 1105 24-25 0.551180708107772 1105 26-27 0.6929948249545674 1105 28-29 0.544714839993965 1105 30-31 0.16278867266477448 1105 32-33 0.33579645560354265 1105 34-35 0.4178867833726798 1105 36-37 0.929251830181272 1105 38-39 0.8865857584188035 1105 40-41 0.8758172528239854 1105 42-43 0.9307371153316097 1105 44-45 1.1944709651265555 1105 46-47 1.2063330651430988 1105 48-49 1.035361418204225 1105 50-51 0.5827607092266476 1105 52-53 0.32179406445387215 1105 54-55 0.813053087631161 1105 56-57 1.4979984192517328 1105 58-59 1.2537505762355146 1105 60-61 0.9063914989254194 1105 62-63 1.1540569941953613 1105 64-65 1.370770739201678 1105 66-67 0.8467393411047404 1105 68-69 1.0624350702796548 1105 70-71 1.139445083506935 1105 72-73 1.6412273390239491 1105 74-75 1.9250629546961768 1105 76-77 2.3148310834758483 1105 78-79 1.9224804969065623 1105 80-81 2.0333993396184624 1105 82-83 2.6877053674609606 1105 84-85 3.088012275454485 1105 86-87 3.0327871558647637 1105 88-89 2.834779219376397 1105 90-91 2.773585418328974 1105 92-93 2.374293589937505 1105 94-95 2.280776109139971 1105 96-97 1.4905580059362507 1105 98-99 2.2215803834192975 1105 100-101 2.406603370030343 1106 1 0.47687578364789474 1106 2 0.27711043355157017 1106 3 -0.03728563400254714 1106 4 -0.029403227690430356 1106 5 0.2121775662793155 1106 6 0.2335140583001163 1106 7 0.32805242774497856 1106 8 0.22112908182982238 1106 9 -0.05106805387490709 1106 10-11 0.43126922034304727 1106 12-13 0.5734801830833831 1106 14-15 0.03723358609922656 1106 16-17 -0.02286523113039607 1106 18-19 0.38602854065098313 1106 20-21 0.23335874809978918 1106 22-23 0.36689931186623426 1106 24-25 0.5878270907987755 1106 26-27 0.6654086180580094 1106 28-29 0.12192917400343362 1106 30-31 0.18003005197512323 1106 32-33 -0.07380462620849215 1106 34-35 -0.27136270952792785 1106 36-37 -0.3184155126179107 1106 38-39 -0.35465832542163866 1106 40-41 -0.2113362292585066 1106 42-43 -0.3144285371362727 1106 44-45 0.15376778730370688 1106 46-47 0.16143786568400387 1106 48-49 0.42731544119273224 1106 50-51 -0.649693651828116 1106 52-53 -1.609105191800353 1106 54-55 -2.3018894411044712 1106 56-57 -1.5077487071850477 1106 58-59 -1.8814759281593503 1106 60-61 -1.5125402116898634 1106 62-63 -2.00807958457408 1106 64-65 -2.338357049844973 1106 66-67 -2.023848894189374 1106 68-69 -1.718700832357257 1106 70-71 -2.932833483092118 1106 72-73 -3.5125928097252057 1106 74-75 -2.925308918190911 1106 76-77 -3.410253433089398 1106 78-79 -3.058452565973763 1106 80-81 -3.3561206062909363 1106 82-83 -2.8298740781103753 1106 84-85 -2.008539448683443 1106 86-87 -1.589254764351601 1106 88-89 -1.1813127346465926 1106 90-91 -0.9483888886351899 1106 92-93 -0.7157672619894733 1106 94-95 -0.7981285561744258 1106 96-97 -1.3805035221232487 1106 98-99 -1.542584592916068 1106 100-101 -1.9320714102265892 1107 1 -0.028871342788885812 1107 2 -0.044728646908200886 1107 3 -0.3131477029680667 1107 4 0.06255079529807972 1107 5 0.01677526742874136 1107 6 0.32546808128861926 1107 7 0.2935696691242953 1107 8 0.1406693117148805 1107 9 0.48916183118256384 1107 10-11 0.10943013988327976 1107 12-13 0.29187098768108655 1107 14-15 0.6349347355245101 1107 16-17 1.1667899412833975 1107 18-19 0.7883273912256925 1107 20-21 0.9574966791342732 1107 22-23 0.41287632336048574 1107 24-25 0.5016201942470602 1107 26-27 0.5217304571384744 1107 28-29 0.12767630044021416 1107 30-31 -0.0038779940018969228 1107 32-33 0.3744712358604758 1107 34-35 0.09070625598931059 1107 36-37 -0.22071436319263427 1107 38-39 -0.40063533691589015 1107 40-41 -0.36076151661482925 1107 42-43 -0.7512101463316725 1107 44-45 -0.4784161207422706 1107 46-47 -0.597182823971167 1107 48-49 -1.049696053060142 1107 50-51 -0.8450959506786901 1107 52-53 -0.5573810538693174 1107 54-55 -0.6926940388056195 1107 56-57 -1.208898132472406 1107 58-59 -1.8240046637915306 1107 60-61 -1.4895517059427377 1107 62-63 -1.6575048719304029 1107 64-65 -1.1889317624886502 1107 66-67 -1.7709753309709804 1107 68-69 -1.8738732461503638 1107 70-71 -1.7201898049311986 1107 72-73 -1.449374418920609 1107 74-75 -0.5747342055472338 1107 76-77 -0.4964603296411205 1107 78-79 -1.1101767039047985 1107 80-81 -1.3101435947966849 1107 82-83 -1.4333223539724393 1107 84-85 -1.4510681843156306 1107 86-87 -1.3363812011332108 1107 88-89 -1.0433817001638346 1107 90-91 -1.0460900380604805 1107 92-93 -1.462893698771083 1107 94-95 -2.7004274067491423 1107 96-97 -3.0069403037324456 1107 98-99 -3.1402857423413515 1107 100-101 -3.2021863527553247 1108 1 -0.5311701933635966 1108 2 -0.48610795725302935 1108 3 -0.017745404117491148 1108 4 -0.5684836874605423 1108 5 -0.3613856521114869 1108 6 -0.10441697618264101 1108 7 -0.3229054266611513 1108 8 -0.6926640216184481 1108 9 -0.07060828375996664 1108 10-11 -0.5258189022623085 1108 12-13 -0.8652171349242792 1108 14-15 -0.6522300154333394 1108 16-17 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0.3443477997147575 2316 84-85 0.16891115185897831 2316 86-87 0.33913216577883887 2316 88-89 -0.35960280309551607 2316 90-91 -0.941285698657147 2316 92-93 0.02968023897914307 2316 94-95 0.02538946009833154 2316 96-97 -0.7517420601576035 2316 98-99 -1.2555511433487148 2316 100-101 -0.7836147396854578 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 325.0 3 63.0 4 91.0 5 58.0 6 66.0 7 89.0 8 122.0 9 172.0 10 248.0 11 356.0 12 557.0 13 753.0 14 972.0 15 1349.0 16 1759.0 17 2459.0 18 2245.0 19 1458.0 20 1478.0 21 1461.0 22 1491.0 23 1647.0 24 1711.0 25 1831.0 26 2056.0 27 2222.0 28 2343.0 29 2831.0 30 3051.0 31 3514.0 32 3982.0 33 4842.0 34 6943.0 35 15659.0 36 8523.0 37 3301.0 38 2907.0 39 738.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.23245629401254 21.7111025470343 16.78286561795653 6.2735755409966245 2 15.157602158197763 29.89827974821026 41.29421794527427 13.649900148317704 3 18.970610557780553 43.50968555515337 30.653993904930932 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6.027021506317663 48.768274533835246 37.869211240479736 7.335492719367358 50-51 6.445418517372804 49.13413217689735 37.110038463558844 7.310410842171003 52-53 5.653935050078211 49.019447622160484 37.42149744355987 7.905119884201434 54-55 5.297932073004885 48.7238153048777 37.27404847925945 8.704204142857977 56-57 5.768759267258993 48.646250481605584 36.604359552135996 8.980630698999429 58-59 5.728610366946239 47.725773785552114 37.135869914143804 9.409745933357847 60-61 5.843655509765693 47.07671293647921 37.76807499678952 9.311556556965572 62-63 6.724842400186786 46.907541442913846 37.76091524632267 8.606700910576699 64-65 6.412882966490979 46.62954467188476 38.533330998616684 8.424241363007582 66-67 5.845751542426206 46.69830318524875 38.74481236976203 8.711132902563024 68-69 5.826070204729741 46.691457626722865 38.92316942889334 8.559302739654056 70-71 5.939594181240806 46.090667102528776 38.43953580685081 9.530202909379597 72-73 5.835244949539169 45.577599943964834 38.44537447248148 10.141780634014511 74-75 6.175981322048737 44.911717495987155 39.084780388151174 9.827520793812928 76-77 6.448769439125765 44.51209545603138 39.108251996450754 9.930883108392099 78-79 6.1861086947637585 44.2105939154752 39.05024899729693 10.55304839246411 80-81 6.185452656037457 44.71040171873267 38.41927969688655 10.684865928343326 82-83 6.389089975013428 44.247834107839246 38.00469373934568 11.358382177801648 84-85 6.898604868367288 43.19187437977935 37.74969353803047 12.159827213822894 86-87 7.445200081725576 42.814278625842796 37.733866495432125 12.006654796999504 88-89 8.132231211983235 41.88703256161489 37.768087515907204 12.21264871049467 90-91 7.926224245607891 41.73874978112415 37.948987334383936 12.386038638884026 92-93 8.257046776114525 41.529856845940074 37.44190934354639 12.77118703439901 94-95 8.78422948274151 41.1384239990193 36.95237205718389 13.124974461055297 96-97 8.983325938207889 40.66112421475444 36.653744657994906 13.701805189042759 98-99 9.852912839617188 40.07147069643055 36.33210517403465 13.74351128991761 100-101 10.88057182567259 39.65463878393609 36.18117156522095 13.283617825170374 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3383.0 1 2258.0 2 1241.0 3 1312.0 4 1137.5 5 1288.5 6 2526.5 7 4896.0 8 6826.0 9 6696.5 10 4781.5 11 3267.0 12 3084.5 13 2983.5 14 2432.5 15 1746.0 16 1252.5 17 955.5 18 812.5 19 705.0 20 645.5 21 642.5 22 644.0 23 671.0 24 684.0 25 722.5 26 806.5 27 854.5 28 926.0 29 1054.0 30 1138.0 31 1192.5 32 1273.0 33 1373.0 34 1427.5 35 1427.5 36 1412.0 37 1402.5 38 1291.0 39 1132.0 40 1030.5 41 911.0 42 808.5 43 703.0 44 616.5 45 573.0 46 601.0 47 732.5 48 1652.5 49 1638.5 50 790.0 51 602.0 52 349.5 53 261.0 54 201.5 55 168.0 56 190.0 57 202.5 58 174.5 59 145.5 60 135.5 61 135.0 62 113.5 63 69.5 64 42.0 65 30.0 66 24.0 67 20.5 68 15.0 69 16.0 70 14.5 71 10.5 72 13.0 73 16.0 74 16.5 75 10.0 76 7.0 77 8.5 78 5.5 79 2.0 80 0.5 81 0.5 82 1.0 83 0.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05135807080410398 2 0.05369252856792688 3 0.03501686645734362 4 0.050190841922192524 5 0.07236819067851015 6 0.06769927515086434 7 0.06886650403277578 8 0.06536481738704142 9 0.06536481738704142 10-11 0.07586987732424451 12-13 0.07295180511946588 14-15 0.06653204626895289 16-17 0.06011228741843989 18-19 0.05719421521366124 20-21 0.06186313074130706 22-23 0.03501686645734362 24-25 0.0215937343153619 26-27 0.015173975464848903 28-29 0.02392819207918481 30-31 0.018092047669627537 32-33 0.007003373291468724 34-35 0.00992144549624736 36-37 0.007003373291468724 38-39 0.012255903260070266 40-41 0.008754216614335905 42-43 0.007003373291468724 44-45 0.001167228881911454 46-47 0.004085301086690089 48-49 5.83614440955727E-4 50-51 0.008754216614335905 52-53 0.008170602173380177 54-55 0.008754216614335905 56-57 0.02567903540205199 58-59 0.007586987732424452 60-61 0.018675662110583264 62-63 0.015173975464848903 64-65 0.011088674378158813 66-67 0.014590361023893175 68-69 0.02742987872491917 70-71 0.022177348756317626 72-73 0.014590361023893175 74-75 0.012255903260070266 76-77 0.024511806520140532 78-79 0.03443325201638789 80-81 0.03326602313447644 82-83 0.031515179811609256 84-85 0.021010119874406173 86-87 0.02392819207918481 88-89 0.02334457763822908 90-91 0.009337831055291632 92-93 0.036184095339255075 94-95 0.022760963197273353 96-97 0.036184095339255075 98-99 0.05077445636314825 100-101 0.06127951630035133 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 85673.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.078192662799246 #Duplication Level Percentage of deduplicated Percentage of total 1 82.91884404709438 30.744808749547698 2 9.204810174400302 6.825954501418183 3 2.87414216457848 3.1970399075554727 4 1.293836177044639 1.9189242818624304 5 0.783856953975949 1.4531999579797603 6 0.49109110369577536 1.092526233469121 7 0.3588742680853743 0.9314486477653403 8 0.2612856513253164 0.7750399775892054 9 0.20462129320657305 0.6828288959182005 >10 1.3253163759994964 9.591119722666418 >50 0.14166089529685827 3.6931121823678406 >100 0.11018069634200088 7.002206062586813 >500 0.012592079581942958 3.0943237659472644 >1k 0.012592079581942958 14.613705601531404 >5k 0.006296039790971479 14.383761511794848 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6412 7.484271590816244 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5911 6.8994899209786045 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4539 5.29805189499609 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3647 4.256883732331073 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3184 3.7164567600060696 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1150 1.342313214198172 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 796 0.9291141900015174 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 727 0.848575397149627 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 603 0.7038390157926068 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 525 0.6127951630035133 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 399 0.46572432388267015 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 343 0.40035950649562874 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 279 0.3256568580532957 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 275 0.3209879425256498 No Hit GTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 265 0.3093156537065353 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 247 0.28830553383212915 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 225 0.2626264984300772 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 224 0.2614592695481657 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 221 0.2579575829024313 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 201 0.23461300526420228 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 184 0.21477011427170753 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 182 0.21243565650788462 No Hit GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 182 0.21243565650788462 No Hit ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 178 0.20776674098023884 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 146 0.1704154167590723 No Hit GAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 144 0.16808095899524939 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 140 0.16341204346760355 No Hit GTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 137 0.15991035682186922 No Hit GTACATGGGGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAAA 136 0.15874312793995776 No Hit GTACATGGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 134 0.15640867017613483 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 133 0.15524144129422338 No Hit GTACATGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 133 0.15524144129422338 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 132 0.15407421241231192 No Hit TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 128 0.1494052968846661 No Hit GAGTACATGGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 125 0.14590361023893175 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 124 0.1447363813570203 No Hit GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 122 0.1424019235931974 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 113 0.1318968636559943 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 112 0.13072963477408284 No Hit GTATCAACGCAGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 109 0.1272279481283485 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 107 0.12489349036452557 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 107 0.12489349036452557 No Hit GGTATCAACGCAGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 106 0.12372626148261412 No Hit GTATCAACGCAGAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAA 105 0.12255903260070267 No Hit GAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 101 0.11789011707305685 No Hit ACGCAGAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 97 0.11322120154541104 No Hit GAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 96 0.11205397266349959 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 94 0.10971951489967668 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 93 0.10855228601776522 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 91 0.10621782825394231 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 90 0.10505059937203087 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 89 0.10388337049011942 No Hit GAGTACATGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 87 0.1015489127262965 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.001167228881911454 0.0 18-19 0.0 0.0 0.0 0.001750843322867181 0.0 20-21 0.0 0.0 0.0 0.004085301086690089 0.0 22-23 0.0 0.0 0.0 0.013423132141981722 0.0 24-25 0.0 0.0 0.0 0.025095420961096262 0.0 26-27 0.0 0.0 0.0 0.0367677097802108 0.0 28-29 0.0 0.0 0.0 0.059528672977484154 0.0 30-31 0.0 0.0 0.0 0.09104385278909341 0.0 32-33 0.0 0.0 0.0 0.11789011707305685 0.0 34-35 0.0 0.0 0.0 0.13481493586077292 0.0 36-37 0.0 0.0 0.0 0.14998891132562184 0.0 38-39 0.0 0.0 0.0 0.16749734455429366 0.0 40-41 0.0 0.0 0.0 0.18325493446009827 0.0 42-43 0.0 0.0 0.0 0.2357802341461137 0.0 44-45 0.0 0.0 0.0 0.2999778226512437 0.0 46-47 0.0 0.0 0.0 0.33207661690380863 0.0 48-49 0.0 0.0 0.0 0.3991922776137173 0.0 50-51 0.0 0.0 0.0 0.45230119174068845 0.0 52-53 0.0 0.0 0.0 0.5357580567973574 0.0 54-55 0.0 0.0 0.0 0.5777782965461697 0.0 56-57 0.0 0.0 0.0 0.6612351616028387 0.0 58-59 0.0 0.0 0.0 0.793132025258833 0.0 60-61 0.0 0.0 0.0 0.8847594924888822 0.0 62-63 0.0 0.0 0.0 1.0184071994677435 0.0 64-65 0.0 0.0 0.0 1.10770020893397 0.0 66-67 0.0 0.0 0.0 1.2156688805107794 0.0 68-69 0.0 0.0 0.0 1.342313214198172 0.0 70-71 0.0 0.0 0.0 1.4327734525463098 0.0 72-73 0.0 0.0 0.0 1.5874312793995773 0.0 74-75 0.0 0.0 0.0 1.723413444142262 0.0 76-77 0.0 0.0 0.0 1.7975324781436393 0.0 78-79 0.0 0.0 0.0 1.8564775366801678 0.0 80-81 0.0 0.0 0.0 1.990708858099985 0.0 82-83 0.0 0.0 0.0 2.093424999708193 0.0 84-85 0.0 0.0 0.0 2.2819324641368928 0.0 86-87 0.0 0.0 0.0 2.541640890362191 0.0 88-89 0.0 0.0 0.0 2.796680401059844 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATA 20 0.002062973 71.28943 1 CTTAGCT 25 0.0049945083 57.03154 2 TATGGGA 25 0.0049945083 57.03154 4 TATATGG 45 1.4991878E-5 52.806984 2 CGCAGAA 80 4.147296E-9 47.49854 9 GGTATCA 1960 0.0 43.889065 1 ATATGGG 55 4.9086957E-5 43.205715 3 CTTGGTA 30 8.262716E-5 39.58212 16-17 GTATCAA 3180 0.0 36.317257 1 GTATATG 70 2.0309427E-4 33.947346 1 GCTTGGT 35 2.0464412E-4 33.92753 14-15 TTGGTAT 35 2.0464412E-4 33.92753 16-17 GAGTAAT 105 1.8750961E-6 31.665693 6 AGTAATG 95 3.454384E-5 29.999077 7 TGCTTGG 40 4.4786738E-4 29.686586 14-15 TGGGCTG 80 4.4601868E-4 29.686586 6 GGGAAAT 65 0.0058151917 29.229872 7 TATCAAC 4075 0.0 27.990942 2 ATGGGCT 120 5.2829837E-6 27.723665 5 GGAGAGT 225 3.6379788E-12 27.443602 8 >>END_MODULE