FastQCFastQC Report
Thu 26 May 2016
SRR936878_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936878_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85673
Sequences flagged as poor quality0
Sequence length101
%GC19

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67727.904473988304367No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66937.812262906633362No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53166.204988736241289No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51626.025235488426926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46485.4252798431244385No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18252.1301927094884037No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14381.6784751321886708No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10711.250102132527167No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8360.9758033452779755No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7630.8905956368984395No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5320.6209657651768935No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3950.46105540835502434No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA3880.4528848061816442No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA3670.4283729996615036No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3420.3991922776137173No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.39452336208607147No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.33966360463623313No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT2380.27780047389492607No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2290.26729541395772294No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT2130.24861975184713972No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1980.2311113186184679No Hit
GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1780.20776674098023884No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1660.19375999439730135No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG1620.18909107886965557No Hit
GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.17975324781436391No Hit
GTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530.17858601893245246No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.1727498745228952No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1370.15991035682186922No Hit
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA1180.13773300806555155No Hit
GAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.13539855030172868No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.13423132141981722No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1120.13072963477408284No Hit
TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.12372626148261412No Hit
GAGTACATGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.12022457483687977No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.1167228881911454No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC980.11438843042732248No Hit
GTACATGGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.10855228601776522No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG920.10738505713585376No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.10388337049011942No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.1015489127262965No Hit
GTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA870.1015489127262965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTT701.8189894E-1161.0552945
TGGGTTG352.7769853E-454.271376
GCAGAAA451.5064672E-552.763839
GGGTGGA405.3671165E-447.487459
CGCAGAA554.9324583E-543.170418
GGTATCA17750.043.073121
GTATATG459.5876487E-442.2110631
TGGGTGG459.5876487E-442.2110638
AATGGGA608.239433E-539.5728763
GTATCAA30300.039.1810651
TAATGGG1002.9820512E-837.9899642
ACATGGA651.3199977E-436.528815
TGGGAGG958.5933607E-734.9907536
TGGGGAT550.002569701334.5363276
GTAATGG1106.900336E-834.5363271
ATGGGTG1456.475602E-1032.749967
TGGGGTA300.003946755531.65830218-19
GGTGGAA300.003946755531.65830210-11
GGGGTGA600.00393583431.6583027
TATCAAC38150.031.1189042
Produced by FastQC (version 0.11.5)