##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936878_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 85673 Sequences flagged as poor quality 0 Sequence length 101 %GC 19 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.89674693310611 31.0 31.0 34.0 30.0 34.0 2 31.82692330139017 31.0 31.0 34.0 30.0 34.0 3 31.799003186534847 31.0 31.0 34.0 30.0 34.0 4 35.70381567121497 37.0 35.0 37.0 33.0 37.0 5 35.70330209050693 37.0 35.0 37.0 35.0 37.0 6 35.88328878409767 37.0 35.0 37.0 35.0 37.0 7 35.8094148681615 37.0 35.0 37.0 35.0 37.0 8 35.864169575011964 37.0 35.0 37.0 35.0 37.0 9 37.3330454168758 39.0 37.0 39.0 34.0 39.0 10-11 37.284943914652224 39.0 37.0 39.0 34.0 39.0 12-13 37.091388185309256 39.0 37.0 39.0 33.0 39.0 14-15 37.71882623463635 39.5 37.0 41.0 32.0 41.0 16-17 37.730335111411996 39.0 37.0 40.5 32.5 41.0 18-19 37.77946377505165 39.0 35.5 41.0 33.5 41.0 20-21 37.92852473941615 40.0 35.0 41.0 33.5 41.0 22-23 37.70352386399449 40.0 35.0 41.0 33.0 41.0 24-25 37.47776428979959 39.0 35.0 41.0 33.0 41.0 26-27 37.072759212353944 38.5 35.0 41.0 33.0 41.0 28-29 36.61635521109334 37.5 35.0 40.5 33.0 41.0 30-31 35.49111738820865 35.5 35.0 39.0 31.5 41.0 32-33 34.552793762328854 35.0 34.5 37.5 30.5 41.0 34-35 34.02125523793961 35.0 33.5 36.5 30.0 40.5 36-37 33.46584104677086 35.0 33.0 35.0 25.0 40.0 38-39 33.00713760461289 35.0 33.0 35.0 23.5 40.0 40-41 32.434535968157995 35.0 33.0 35.0 21.5 40.0 42-43 31.821145518424707 35.0 33.0 35.0 19.5 40.0 44-45 31.10592602103346 35.0 33.0 35.0 15.5 40.0 46-47 30.110577428127883 35.0 31.0 35.0 3.5 40.0 48-49 29.288019562756062 35.0 28.5 35.0 2.0 39.0 50-51 28.96374587092783 35.0 27.0 35.0 2.0 39.0 52-53 28.724458113991574 35.0 27.0 35.0 2.0 39.0 54-55 28.354510756014147 35.0 27.0 35.0 2.0 38.0 56-57 28.055875246577102 35.0 25.5 35.0 2.0 38.0 58-59 27.703477174839215 35.0 24.5 35.0 2.0 38.0 60-61 27.323818472564284 34.5 25.0 35.0 2.0 38.0 62-63 26.90379699555286 33.5 22.0 35.0 2.0 37.0 64-65 26.440477163166925 33.0 21.0 35.0 2.0 36.5 66-67 25.927380855111878 33.0 20.0 35.0 2.0 36.0 68-69 25.505766110676642 33.0 16.5 35.0 2.0 35.5 70-71 25.189061898147607 33.0 9.5 35.0 2.0 35.0 72-73 24.963465735996174 33.0 2.0 35.0 2.0 35.0 74-75 24.518681498254992 33.0 2.0 35.0 2.0 35.0 76-77 23.0410339313436 30.0 2.0 34.0 2.0 35.0 78-79 23.610419852228823 33.0 2.0 35.0 2.0 35.0 80-81 23.202572572455733 33.0 2.0 35.0 2.0 35.0 82-83 22.80964831393788 33.0 2.0 35.0 2.0 35.0 84-85 22.244983833879985 32.5 2.0 35.0 2.0 35.0 86-87 21.571889626836928 31.0 2.0 35.0 2.0 35.0 88-89 20.84419828884246 31.0 2.0 35.0 2.0 35.0 90-91 20.043432586695925 29.0 2.0 35.0 2.0 35.0 92-93 19.28301215085266 28.0 2.0 35.0 2.0 35.0 94-95 18.453736883265442 25.5 2.0 34.0 2.0 35.0 96-97 17.552501955108376 21.0 2.0 33.5 2.0 35.0 98-99 16.667374785521694 2.0 2.0 33.0 2.0 35.0 100-101 14.549513849170683 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5348877850344742 1101 2 0.5466134528458788 1101 3 0.4882570994877291 1101 4 0.4099321479396991 1101 5 0.5205549836906371 1101 6 0.5787869439119646 1101 7 0.5738516352420575 1101 8 0.4855526231615457 1101 9 0.5126767890043666 1101 10-11 0.46731036178599794 1101 12-13 0.43900933234204587 1101 14-15 0.8580383675532417 1101 16-17 0.5290458751488032 1101 18-19 0.5991365982835717 1101 20-21 0.4473262436002514 1101 22-23 0.502990293031381 1101 24-25 0.7978867300325518 1101 26-27 0.5037372359057102 1101 28-29 0.39490527232594985 1101 30-31 0.1758280918731785 1101 32-33 0.4767196362503867 1101 34-35 0.6472594943213963 1101 36-37 1.4391543213346765 1101 38-39 1.1966699907887701 1101 40-41 0.9977865821887377 1101 42-43 1.0449077958357869 1101 44-45 0.4114867842296519 1101 46-47 0.27918064670966913 1101 48-49 0.18246119011427808 1101 50-51 0.4016925734691448 1101 52-53 0.189980691431213 1101 54-55 0.8357870311487972 1101 56-57 0.84296011291379 1101 58-59 0.9268657015744672 1101 60-61 1.1209047220936128 1101 62-63 0.7769651078599615 1101 64-65 0.5691250440542497 1101 66-67 0.3455401728859222 1101 68-69 0.6092490965427899 1101 70-71 0.50925068804003 1101 72-73 0.7479832659677044 1101 74-75 0.6027896827834347 1101 76-77 0.48093010187743346 1101 78-79 1.082330701166562 1101 80-81 1.293837455914467 1101 82-83 1.3314564497918369 1101 84-85 0.92238235968588 1101 86-87 1.0337482239389892 1101 88-89 1.0402581388897616 1101 90-91 0.3599105834832592 1101 92-93 0.5806822738245359 1101 94-95 0.26905301672396575 1101 96-97 0.7279797188331152 1101 98-99 1.4189777965088517 1101 100-101 1.3039208635202932 1102 1 0.1847972053243332 1102 2 0.2907257716864571 1102 3 0.06706144731381514 1102 4 0.3279575102585355 1102 5 0.02961295470512937 1102 6 0.4891130308684879 1102 7 0.48191322944495596 1102 8 0.25049827533545965 1102 9 0.4864086730623356 1102 10-11 0.18356941975701346 1102 12-13 0.22229737582031106 1102 14-15 0.08765793277063239 1102 16-17 0.583167251960397 1102 18-19 0.49270543886328966 1102 20-21 0.6126342146147508 1102 22-23 0.7092855828864444 1102 24-25 0.7238378169890751 1102 26-27 0.8911919460506468 1102 28-29 0.8930936781230585 1102 30-31 0.6962085266557878 1102 32-33 1.015668911612714 1102 34-35 0.7722594943213963 1102 36-37 0.972895263363661 1102 38-39 0.7107098458612242 1102 40-41 0.6737376691452539 1102 42-43 1.2668280856908503 1102 44-45 1.1275193929253078 1102 46-47 1.209660719173435 1102 48-49 2.01194488576645 1102 50-51 1.8167740952082774 1102 52-53 1.2196455465036742 1102 54-55 1.4492381181053204 1102 56-57 1.741057939000747 1102 58-59 1.6066664262121506 1102 60-61 1.728015229339988 1102 62-63 1.706992281773008 1102 64-65 2.0736540295614994 1102 66-67 1.6136561149149102 1102 68-69 1.9240135892964148 1102 70-71 2.2920858329675653 1102 72-73 2.493227831185095 1102 74-75 2.102563233508075 1102 76-77 2.208058725065843 1102 78-79 2.0100933823259837 1102 80-81 1.8359570211318577 1102 82-83 1.9444546381976338 1102 84-85 1.68800735968588 1102 86-87 2.2377790210404385 1102 88-89 2.696281689614402 1102 90-91 3.0372203660919546 1102 92-93 2.6545047375926494 1102 94-95 2.755013161651501 1102 96-97 3.6512134144852872 1102 98-99 4.404032144334938 1102 100-101 2.966058544679713 1103 1 0.3557883226688823 1103 2 0.149772054996415 1103 3 0.06823021776729732 1103 4 0.09104773933754728 1103 5 -0.23616544641689075 1103 6 0.3885987718689563 1103 7 0.09166077502700887 1103 8 0.2063187521938019 1103 9 0.4448004449183429 1103 10-11 0.3401262220010537 1103 12-13 0.2943520742775334 1103 14-15 1.063347507338186 1103 16-17 0.520813348267076 1103 18-19 0.7580747703265871 1103 20-21 0.638522480159395 1103 22-23 0.7581986263647096 1103 24-25 0.7967106547637357 1103 26-27 0.5465799778411977 1103 28-29 0.6126472078098217 1103 30-31 0.3172931456366186 1103 32-33 -0.01723197665283749 1103 34-35 0.1082809996977403 1103 36-37 0.5112309342378936 1103 38-39 0.5363877327242434 1103 40-41 -0.23054003071449358 1103 42-43 0.3735368280938509 1103 44-45 0.41585506379954396 1103 46-47 0.05690242090321718 1103 48-49 0.31670178151212625 1103 50-51 0.02014015411430492 1103 52-53 0.6797320355172332 1103 54-55 -0.5464374312167948 1103 56-57 0.1100972096879822 1103 58-59 -0.3416154812212291 1103 60-61 0.04042757155597698 1103 62-63 0.4817702153868417 1103 64-65 -0.13988033228983454 1103 66-67 0.49070146320850583 1103 68-69 0.3992356556825776 1103 70-71 0.5725907418034701 1103 72-73 0.9030571906988882 1103 74-75 0.9897184462242983 1103 76-77 1.0083158545656055 1103 78-79 1.1066922603063496 1103 80-81 1.653044445161779 1103 82-83 1.942175535813341 1103 84-85 1.7001041338794316 1103 86-87 1.3961474174873771 1103 88-89 1.3683831388897651 1103 90-91 0.40812966950476337 1103 92-93 0.41367958565249197 1103 94-95 0.9360557048960096 1103 96-97 1.2355402027040832 1103 98-99 0.45762026962713165 1103 100-101 1.370453121584811 1104 1 -0.027971410367822358 1104 2 0.15904161376541737 1104 3 0.07230882362566149 1104 4 0.05396950426153779 1104 5 -0.021111682976034274 1104 6 -0.11158661930642921 1104 7 -0.018461583148742022 1104 8 0.11881411741441639 1104 9 -0.04982321099563336 1104 10-11 -0.31411923591515034 1104 12-13 -0.2892593458188699 1104 14-15 -0.5755104830214677 1104 16-17 -0.08930900990866775 1104 18-19 0.03412223046748153 1104 20-21 -0.2901019173192836 1104 22-23 0.08003770682447708 1104 24-25 0.20216115531990653 1104 26-27 0.41124441981376236 1104 28-29 0.44375584703859516 1104 30-31 0.39385970106858537 1104 32-33 0.25473687762970343 1104 34-35 0.5739836322524283 1104 36-37 0.19903219489788881 1104 38-39 0.038983209179569656 1104 40-41 -0.11374359022505587 1104 42-43 1.0413158418127928 1104 44-45 0.8910126462986163 1104 46-47 0.7387711639510464 1104 48-49 1.2336465349418653 1104 50-51 0.8505072286415576 1104 52-53 1.3352752316610967 1104 54-55 0.8111821460913262 1104 56-57 0.601939997971261 1104 58-59 0.5313915636434352 1104 60-61 1.2248917910591288 1104 62-63 1.2326044756760517 1104 64-65 1.140245733709424 1104 66-67 0.6328964947250064 1104 68-69 1.0851830045887674 1104 70-71 1.5572032742469233 1104 72-73 1.6360938981516142 1104 74-75 1.562021004622519 1104 76-77 0.517029239808469 1104 78-79 1.2524097241550685 1104 80-81 1.612443777753544 1104 82-83 1.6980153578378108 1104 84-85 1.4279498884215123 1104 86-87 2.0522467871573795 1104 88-89 2.0810268170506845 1104 90-91 2.5777625949775107 1104 92-93 2.729029974973958 1104 94-95 2.942544396034311 1104 96-97 2.0165932245802445 1104 98-99 1.9107163022559774 1104 100-101 1.781650748577766 1105 1 0.2046181771913389 1105 2 -0.10032282166392648 1105 3 0.3169335700759639 1105 4 0.02476057931224318 1105 5 0.31222165035730143 1105 6 0.34128694391196746 1105 7 0.3766702626930396 1105 8 0.23592027022036888 1105 9 0.5227258086122077 1105 10-11 0.1374696755114897 1105 12-13 -0.17073331471677733 1105 14-15 0.19149424990617803 1105 16-17 0.10190126730566362 1105 18-19 0.2955826767149503 1105 20-21 -0.28563944267425256 1105 22-23 -0.141801373635289 1105 24-25 0.23630584767960983 1105 26-27 0.18345537316061922 1105 28-29 0.17627782134555758 1105 30-31 0.21001926834376405 1105 32-33 0.2617686558582335 1105 34-35 0.4516712590272789 1105 36-37 0.42046559584447607 1105 38-39 0.3340474417691581 1105 40-41 0.22297040571815074 1105 42-43 0.043682305639702435 1105 44-45 1.030972078347304 1105 46-47 2.0325507447488853 1105 48-49 2.354336190114278 1105 50-51 2.5121705146456144 1105 52-53 2.9723949071174864 1105 54-55 2.874941442913503 1105 56-57 3.0970042305608487 1105 58-59 3.010566681966626 1105 60-61 3.5882454083681203 1105 62-63 3.3554577549187847 1105 64-65 3.250252495034644 1105 66-67 2.643579388572199 1105 68-69 2.8784892926212216 1105 70-71 2.137865884118458 1105 72-73 2.442652383614764 1105 74-75 3.325645074940301 1105 76-77 3.2790305920735143 1105 78-79 3.47614197567637 1105 80-81 4.0015580441497605 1105 82-83 3.497203998811443 1105 84-85 2.2424191243917626 1105 86-87 2.436628125899773 1105 88-89 2.5487752957525096 1105 90-91 3.025903230542081 1105 92-93 3.110706783628455 1105 94-95 3.374322624567103 1105 96-97 3.570075307068411 1105 98-99 3.5524336788617923 1105 100-101 3.1629894909712757 1106 1 0.23639640572413256 1106 2 0.2165128781332335 1106 3 0.34817089259117395 1106 4 0.2723603088592341 1106 5 0.24325613311592065 1106 6 0.31370073701540946 1106 7 0.46429703754090923 1106 8 0.31421641626499053 1106 9 0.4674181683147083 1106 10-11 0.5536968560388758 1106 12-13 0.08430387257192962 1106 14-15 0.2578228503118609 1106 16-17 0.5083921395166158 1106 18-19 -0.51185478102677 1106 20-21 0.4225417608416322 1106 22-23 0.3673940286635613 1106 24-25 0.22514966106703582 1106 26-27 0.4744628106183555 1106 28-29 0.4839857320960661 1106 30-31 0.3133999309536364 1106 32-33 0.12255296958372952 1106 34-35 0.4303054713328933 1106 36-37 0.0668482868519149 1106 38-39 0.18266137009911176 1106 40-41 0.31729089253356335 1106 42-43 0.6620054969852056 1106 44-45 -0.5112862042760966 1106 46-47 -1.1347920544397603 1106 48-49 -1.0824454190811252 1106 50-51 -1.2701824265308552 1106 52-53 -1.4693224694883291 1106 54-55 -1.4934155550580996 1106 56-57 -1.7198990824885065 1106 58-59 -1.3306774018738068 1106 60-61 -1.3670622319293777 1106 62-63 -1.480039202484864 1106 64-65 -1.279294496175634 1106 66-67 -1.413080516769245 1106 68-69 -1.2366560758710001 1106 70-71 -2.1094633924197446 1106 72-73 -2.283446331733444 1106 74-75 -1.8345307195154135 1106 76-77 -2.207108691226015 1106 78-79 -3.2188546436610217 1106 80-81 -2.968015992361398 1106 82-83 -2.1123294697483956 1106 84-85 -1.6237742495095198 1106 86-87 -1.5626957415782528 1106 88-89 -2.384490424328625 1106 90-91 -1.7958006234132924 1106 92-93 -2.0123493353708675 1106 94-95 -2.5632027304024696 1106 96-97 -2.3339814880634364 1106 98-99 -3.0892836977440226 1106 100-101 -2.5057055732613147 1107 1 0.19041939422988108 1107 2 -0.31222275405067634 1107 3 0.32518238684405176 1107 4 0.24937180311211193 1107 5 0.24325613311592065 1107 6 0.35967774850966805 1107 7 0.05050393409263165 1107 8 0.0728371059201649 1107 9 0.19155609934919227 1107 10-11 0.059443982475663404 1107 12-13 0.44637283808916806 1107 14-15 0.2923056089325513 1107 16-17 0.663564553309719 1107 18-19 0.6030877477088623 1107 20-21 0.3420819907266903 1107 22-23 0.40762391372103224 1107 24-25 0.6504370173888745 1107 26-27 0.35952028188273033 1107 28-29 -0.10222116445565632 1107 30-31 -0.3532667357130279 1107 32-33 0.1915184868251032 1107 34-35 -0.05245314935675793 1107 36-37 -0.27223217291820134 1107 38-39 -0.38055702070548847 1107 40-41 -1.0907550844779266 1107 42-43 -0.7000634685320328 1107 44-45 -0.011286204276096612 1107 46-47 -0.3531828590374566 1107 48-49 -0.8468132351730766 1107 50-51 -0.8334008173354519 1107 52-53 -0.9233454579940741 1107 54-55 -1.981921302184535 1107 56-57 -1.9727726457069004 1107 58-59 -1.48584981566691 1107 60-61 -1.217636944573055 1107 62-63 -0.28463690363428995 1107 64-65 -0.4861910478997693 1107 66-67 -0.5222759190680968 1107 68-69 0.10817151033589667 1107 70-71 -0.4255553464427315 1107 72-73 -2.0248256420782695 1107 74-75 -2.0874042827338037 1107 76-77 -1.4542351280076211 1107 78-79 -1.115406367798954 1107 80-81 -0.4105447279935781 1107 82-83 -0.2904903892886246 1107 84-85 -1.198486893187681 1107 86-87 -1.2638451668656074 1107 88-89 -0.6948352519148315 1107 90-91 -0.2958006234132924 1107 92-93 -0.2882114043363835 1107 94-95 0.18392370637914013 1107 96-97 -0.6040964305921719 1107 98-99 0.17508411834793236 1107 100-101 0.2529151163938561 1108 1 0.06474889614558776 1108 2 -0.22908099159856832 1108 3 -0.024242900512270182 1108 4 0.2953488146063634 1108 5 -0.5211116829760343 1108 6 -0.10315750053248252 1108 7 0.2328794130198375 1108 8 -0.11375293239400719 1108 9 0.45017678900436664 1108 10-11 0.18224091734155934 1108 12-13 -0.4210601121024027 1108 14-15 -0.16765607689119832 1108 16-17 0.12314309737102036 1108 18-19 0.04983104272802308 1108 20-21 -0.10197931195529719 1108 22-23 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-0.5169415724794675 2316 58-59 -0.08355096509219706 2316 60-61 0.37225069587638515 2316 62-63 -0.468996970791725 2316 64-65 -0.7850416226124146 2316 66-67 -1.1582051454661375 2316 68-69 -1.015165697464699 2316 70-71 0.1543232535830974 2316 72-73 -0.1847765654929674 2316 74-75 0.3383350947684569 2316 76-77 0.5814099707912952 2316 78-79 0.8926654389942783 2316 80-81 0.7689076806335677 2316 82-83 0.9235093524135571 2316 84-85 0.004018595640935985 2316 86-87 -0.14631965995614138 2316 88-89 -1.129744201934205 2316 90-91 -0.4007986861796624 2316 92-93 -0.4894254040780872 2316 94-95 -0.17207526042959032 2316 96-97 -0.2592042699309296 2316 98-99 -0.4493508551765437 2316 100-101 0.41745082606711037 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 6.0 5 18.0 6 61.0 7 102.0 8 129.0 9 256.0 10 387.0 11 510.0 12 695.0 13 937.0 14 1186.0 15 1596.0 16 2221.0 17 2799.0 18 2545.0 19 1759.0 20 1524.0 21 1547.0 22 1587.0 23 1759.0 24 2005.0 25 2036.0 26 2235.0 27 2558.0 28 2872.0 29 3235.0 30 3647.0 31 3898.0 32 4507.0 33 5446.0 34 8060.0 35 13367.0 36 4117.0 37 3210.0 38 2576.0 39 280.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.22068095838588 20.238428844986224 23.159824389332588 6.381065807295315 2 14.213843819306643 29.04867514882689 43.317380646667445 13.420100385199019 3 17.401505865872878 38.492966789237144 37.39333450067122 6.712192844218759 4 8.119244102576074 24.529314953369205 38.36331166178376 28.988129282270958 5 5.5536750201346985 44.4410724498967 32.31589882460052 17.689353705368084 6 15.040911372310998 41.741272046035505 22.827495243542305 20.39032133811119 7 14.761943669534158 47.72565452359554 23.149650414949868 14.362751391920442 8 26.675848867204373 29.439846859570697 23.084285597562825 20.80001867566211 9 29.512215050249203 17.102237577766623 24.59818145740198 28.787365914582193 10-11 21.53362202794346 33.75217396379256 24.676969406931004 20.037234601332976 12-13 28.29829701306129 35.57538547733825 29.344134091253952 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35.32515437683122 53.564966673281425 4.9925875774802435 40-41 5.39783339559208 36.00637373926037 53.48512794172581 5.110664923421741 42-43 5.073478773447258 36.68686019773319 52.707450595884254 5.532210432935299 44-45 5.187165151214502 36.9457121847023 51.54132573856407 6.325796925519126 46-47 5.265953100743525 36.79397243005381 50.27780047389493 7.66227399530774 48-49 5.560159678774862 36.85801662153329 49.61597721542628 7.965846484265571 50-51 6.027605124164697 37.22372990166039 48.79453733695176 7.954127637223146 52-53 5.3115370249323 37.38210850686339 48.74871603324307 8.557638434961246 54-55 4.960780651788216 37.27063684751144 48.46857783173032 9.300004668970026 56-57 5.526245141409778 37.17332181667503 47.4268439298262 9.87358911208899 58-59 5.294550208350356 36.47006641532338 47.982444877616054 10.252938498710213 60-61 5.589859115473953 36.06328714997724 48.239818846077526 10.107034888471281 62-63 6.384741984055653 36.070874137709666 48.086911862547126 9.457472015687555 64-65 6.178466997787986 35.96654546303484 48.837670789146536 9.017316750030641 66-67 5.626043210813209 35.92380329858882 49.002602920406666 9.447550570191309 68-69 5.603865862056891 36.028270283519895 48.86486991234111 9.502993942082103 70-71 5.577668316369409 35.79115697077225 48.21236810159679 10.418806611261555 72-73 5.58217718918351 35.032772819863304 48.30882910856762 11.076220882385558 74-75 5.759308976304424 34.76246060464573 48.50706198202405 10.971168437025797 76-77 5.820454757680456 34.47042207489028 48.36177514240359 11.34734802502568 78-79 5.588467038258383 34.31406309277147 48.52423614556279 11.573233723407359 80-81 5.697244172609807 34.95033441107467 47.36906610017158 11.983355316143943 82-83 5.43766268632326 34.74863139218638 46.79004563971472 13.023660281775632 84-85 5.793024558782332 33.96442244840788 46.3401111214866 13.902441871323187 86-87 6.03690777724604 33.457448671109915 46.2852940833168 14.220349468327246 88-89 6.627096683825331 32.57111507978196 46.52682938217133 14.274958854221381 90-91 6.359646563094558 32.41511327956299 46.39267914045265 14.832561016889803 92-93 6.22132994058805 32.41394605068108 46.005742766099004 15.358981242631867 94-95 6.495045113396286 32.27562942817457 45.36259965216579 15.866725806263352 96-97 6.626358362611325 31.613810652130773 44.82509075204557 16.93474023321233 98-99 6.988199316003875 31.178434279177804 44.75097171804419 17.08239468677413 100-101 7.2537438866387305 30.96833308043374 44.87995050949541 16.89797252343212 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5801.0 1 3853.0 2 1954.5 3 1909.5 4 1439.0 5 1307.5 6 2482.0 7 4900.5 8 6629.0 9 6320.5 10 4442.0 11 2862.5 12 2554.0 13 2333.0 14 1872.5 15 1392.0 16 1058.0 17 851.5 18 737.0 19 659.0 20 614.0 21 597.5 22 591.5 23 587.5 24 603.5 25 646.5 26 728.0 27 799.5 28 867.0 29 966.5 30 1044.0 31 1096.0 32 1169.0 33 1244.0 34 1324.0 35 1389.5 36 1436.5 37 1469.0 38 1403.0 39 1222.5 40 1086.5 41 977.5 42 863.0 43 805.5 44 748.5 45 669.0 46 660.5 47 788.5 48 1721.0 49 1675.0 50 781.5 51 621.0 52 372.5 53 237.5 54 184.5 55 169.5 56 160.0 57 141.0 58 122.5 59 103.0 60 88.5 61 94.0 62 93.5 63 58.5 64 26.5 65 16.5 66 13.0 67 13.0 68 10.5 69 10.0 70 12.0 71 9.5 72 8.0 73 13.5 74 15.5 75 11.5 76 9.5 77 9.0 78 5.0 79 4.0 80 4.0 81 2.0 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03384963757543217 2 0.003501686645734362 3 0.009337831055291632 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.005252529968601543 20-21 0.0 22-23 0.0 24-25 0.002918072204778635 26-27 0.003501686645734362 28-29 0.00583614440955727 30-31 0.036184095339255075 32-33 0.0 34-35 0.002334457763822908 36-37 0.015173975464848903 38-39 0.007003373291468724 40-41 0.010505059937203087 42-43 0.002334457763822908 44-45 0.0 46-47 0.0 48-49 0.001167228881911454 50-51 5.83614440955727E-4 52-53 0.001167228881911454 54-55 0.001167228881911454 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.005252529968601543 66-67 0.0 68-69 0.0 70-71 0.001167228881911454 72-73 0.008754216614335905 74-75 0.003501686645734362 76-77 0.001167228881911454 78-79 0.006419758850512997 80-81 0.0 82-83 0.002334457763822908 84-85 0.001167228881911454 86-87 0.0 88-89 0.002334457763822908 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 85673.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.92487714916018 #Duplication Level Percentage of deduplicated Percentage of total 1 82.82329713721619 26.441235861940164 2 8.986874337318563 5.738097183476707 3 2.983437534276626 2.8573763029192394 4 1.4405323388541553 1.8395527178924516 5 0.786077291506709 1.254771048054813 6 0.449709334210815 0.8614149148506531 7 0.3290556103981573 0.735354195604216 8 0.2851815290117363 0.7283508223127473 9 0.19743336623889435 0.5672732366089667 >10 1.4259076450586816 8.671343363720192 >50 0.16818397864794707 3.822674588260012 >100 0.08409198932397353 5.5641800800719015 >500 0.010968520346605243 2.4873647473533085 >1k 0.014624693795473659 10.48404981732868 >5k 0.014624693795473659 27.946961119605945 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6772 7.904473988304367 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6693 7.812262906633362 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5316 6.204988736241289 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5162 6.025235488426926 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4648 5.4252798431244385 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1825 2.1301927094884037 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1438 1.6784751321886708 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1071 1.250102132527167 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 836 0.9758033452779755 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 763 0.8905956368984395 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 532 0.6209657651768935 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 395 0.46105540835502434 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 388 0.4528848061816442 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 367 0.4283729996615036 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 342 0.3991922776137173 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 338 0.39452336208607147 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 291 0.33966360463623313 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 238 0.27780047389492607 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 229 0.26729541395772294 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 213 0.24861975184713972 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 198 0.2311113186184679 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 178 0.20776674098023884 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 166 0.19375999439730135 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 162 0.18909107886965557 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 154 0.17975324781436391 No Hit GTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 153 0.17858601893245246 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 148 0.1727498745228952 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 137 0.15991035682186922 No Hit GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 118 0.13773300806555155 No Hit GAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 116 0.13539855030172868 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 115 0.13423132141981722 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 112 0.13072963477408284 No Hit TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 106 0.12372626148261412 No Hit GAGTACATGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 103 0.12022457483687977 No Hit ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 100 0.1167228881911454 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 98 0.11438843042732248 No Hit GTACATGGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 93 0.10855228601776522 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 92 0.10738505713585376 No Hit GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 89 0.10388337049011942 No Hit ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 87 0.1015489127262965 No Hit GTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 87 0.1015489127262965 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.001167228881911454 0.0 18-19 0.0 0.0 0.0 0.001750843322867181 0.0 20-21 0.0 0.0 0.0 0.004085301086690089 0.0 22-23 0.0 0.0 0.0 0.012839517701025992 0.0 24-25 0.0 0.0 0.0 0.024511806520140535 0.0 26-27 0.0 0.0 0.0 0.03443325201638789 0.0 28-29 0.0 0.0 0.0 0.05719421521366125 0.0 30-31 0.0 0.0 0.0 0.08695855170240332 0.0 32-33 0.0 0.0 0.0 0.11263758710445532 0.0 34-35 0.0 0.0 0.0 0.13014602033312714 0.0 36-37 0.0 0.0 0.0 0.14765445356179893 0.0 38-39 0.0 0.0 0.0 0.16457927234951503 0.0 40-41 0.0 0.0 0.0 0.18208770557818682 0.0 42-43 0.0 0.0 0.0 0.23928192079184807 0.0 44-45 0.0 0.0 0.0 0.309899268147491 0.0 46-47 0.0 0.0 0.0 0.3460833634867461 0.0 48-49 0.0 0.0 0.0 0.4155334819604776 0.0 50-51 0.0 0.0 0.0 0.47039323941031597 0.0 52-53 0.0 0.0 0.0 0.5596862488765422 0.0 54-55 0.0 0.0 0.0 0.6046245608301332 0.0 56-57 0.0 0.0 0.0 0.6915831125325365 0.0 58-59 0.0 0.0 0.0 0.8304833494799995 0.0 60-61 0.0 0.0 0.0 0.9273633466786502 0.0 62-63 0.0 0.0 0.0 1.068598041389936 0.0 64-65 0.0 0.0 0.0 1.1619763519428525 0.0 66-67 0.0 0.0 0.0 1.2769483968111306 0.0 68-69 0.0 0.0 0.0 1.413514175994771 0.0 70-71 0.0 0.0 0.0 1.5063088721067315 0.0 72-73 0.0 0.0 0.0 1.6691373011333792 0.0 74-75 0.0 0.0 0.0 1.8144572969313553 0.0 76-77 0.0 0.0 0.0 1.896163318665157 0.0 78-79 0.0 0.0 0.0 1.9574428349655082 0.0 80-81 0.0 0.0 0.0 2.0940086141491485 0.0 82-83 0.0 0.0 0.0 2.2008100568440465 0.0 84-85 0.0 0.0 0.0 2.390484750154658 0.0 86-87 0.0 0.0 0.0 2.661865465199071 0.0 88-89 0.0 0.0 0.0 2.915737747014812 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGTT 70 1.8189894E-11 61.055294 5 TGGGTTG 35 2.7769853E-4 54.27137 6 GCAGAAA 45 1.5064672E-5 52.76383 9 GGGTGGA 40 5.3671165E-4 47.48745 9 CGCAGAA 55 4.9324583E-5 43.17041 8 GGTATCA 1775 0.0 43.07312 1 GTATATG 45 9.5876487E-4 42.211063 1 TGGGTGG 45 9.5876487E-4 42.211063 8 AATGGGA 60 8.239433E-5 39.572876 3 GTATCAA 3030 0.0 39.181065 1 TAATGGG 100 2.9820512E-8 37.989964 2 ACATGGA 65 1.3199977E-4 36.52881 5 TGGGAGG 95 8.5933607E-7 34.990753 6 TGGGGAT 55 0.0025697013 34.536327 6 GTAATGG 110 6.900336E-8 34.536327 1 ATGGGTG 145 6.475602E-10 32.74996 7 TGGGGTA 30 0.0039467555 31.658302 18-19 GGTGGAA 30 0.0039467555 31.658302 10-11 GGGGTGA 60 0.003935834 31.658302 7 TATCAAC 3815 0.0 31.118904 2 >>END_MODULE