##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936877_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 85242 Sequences flagged as poor quality 0 Sequence length 101 %GC 19 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.75288003566317 33.0 31.0 34.0 30.0 34.0 2 31.57646465357453 31.0 31.0 34.0 30.0 34.0 3 31.40105816381596 31.0 31.0 34.0 28.0 34.0 4 35.17953590952817 37.0 35.0 37.0 33.0 37.0 5 35.20676427113395 37.0 35.0 37.0 33.0 37.0 6 35.52923441495976 37.0 35.0 37.0 33.0 37.0 7 35.54625654020319 37.0 35.0 37.0 33.0 37.0 8 35.50979564064663 37.0 35.0 37.0 33.0 37.0 9 36.91789258816077 39.0 37.0 39.0 33.0 39.0 10-11 36.80966542314821 39.0 37.0 39.0 32.0 39.0 12-13 36.56140752211351 39.0 37.0 39.0 32.0 39.0 14-15 37.201168438093895 39.0 36.5 41.0 31.5 41.0 16-17 37.25523802820206 39.0 36.5 40.5 32.0 41.0 18-19 37.42743014007179 39.0 36.0 41.0 33.0 41.0 20-21 37.622363388939725 40.0 35.0 41.0 33.0 41.0 22-23 37.521175007625345 40.0 35.0 41.0 33.0 41.0 24-25 37.28108209568053 39.0 35.0 41.0 33.0 41.0 26-27 37.031222871354494 39.0 35.0 41.0 33.0 41.0 28-29 36.685941202693506 38.0 35.0 41.0 33.0 41.0 30-31 35.911135355810515 36.5 35.0 39.5 33.0 41.0 32-33 35.09842565871284 35.0 35.0 39.0 31.5 41.0 34-35 34.65345135027334 35.0 35.0 38.0 31.0 41.0 36-37 34.193824640435466 35.0 35.0 37.0 31.0 40.5 38-39 33.772676614814294 35.0 35.0 35.5 30.0 40.0 40-41 33.31446939302222 35.0 35.0 35.0 27.0 40.0 42-43 32.79730649210483 35.0 34.5 35.0 23.0 40.0 44-45 32.189601370216565 35.0 33.0 35.0 20.5 40.0 46-47 31.423459092935406 35.0 33.0 35.0 15.5 40.0 48-49 30.637209356889795 35.0 33.0 35.0 2.0 39.0 50-51 29.43759531686258 34.0 29.5 35.0 2.0 38.5 52-53 29.637936697871943 35.0 31.0 35.0 2.0 38.5 54-55 29.832600126698107 35.0 32.5 35.0 2.0 39.0 56-57 29.623331221698223 35.0 32.5 35.0 2.0 39.0 58-59 29.32045822481875 35.0 31.0 35.0 2.0 39.0 60-61 29.011572933530417 35.0 31.0 35.0 2.0 39.0 62-63 28.605288472818565 35.0 30.5 35.0 2.0 38.0 64-65 28.199807606578915 35.0 28.5 35.0 2.0 37.5 66-67 27.7868656296192 35.0 27.0 35.0 2.0 37.0 68-69 27.392541235541167 35.0 26.0 35.0 2.0 36.5 70-71 26.98667323619812 35.0 24.5 35.0 2.0 36.0 72-73 26.538461087257456 35.0 22.0 35.0 2.0 35.0 74-75 26.076863517984094 35.0 17.5 35.0 2.0 35.0 76-77 25.57679313014711 34.5 3.5 35.0 2.0 35.0 78-79 25.089134464231247 34.0 2.0 35.0 2.0 35.0 80-81 24.522007930362967 33.0 2.0 35.0 2.0 35.0 82-83 23.935730039182562 33.0 2.0 35.0 2.0 35.0 84-85 23.333591422068935 33.0 2.0 35.0 2.0 35.0 86-87 22.712864550339035 33.0 2.0 35.0 2.0 35.0 88-89 22.045388423547077 33.0 2.0 35.0 2.0 35.0 90-91 21.332881678046036 31.5 2.0 35.0 2.0 35.0 92-93 20.76438844700969 31.0 2.0 35.0 2.0 35.0 94-95 20.31848149973018 31.0 2.0 35.0 2.0 35.0 96-97 19.624310785762887 29.5 2.0 35.0 2.0 35.0 98-99 18.87696792660895 27.5 2.0 35.0 2.0 35.0 100-101 17.640763942657376 22.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.1768298279756344 1101 2 -0.30989955249177825 1101 3 -0.4343959868629028 1101 4 -0.8162235333606205 1101 5 -0.3098862821835695 1101 6 -0.2802102530763051 1101 7 -0.4756184344232608 1101 8 -0.4551966084910788 1101 9 -0.01188241941421353 1101 10-11 -0.21234314233443996 1101 12-13 -0.4089641287983099 1101 14-15 -0.6609179892506134 1101 16-17 -0.37479946623555804 1101 18-19 -0.0023280995961059148 1101 20-21 -0.0983881509297575 1101 22-23 -0.680097035218246 1101 24-25 -0.26029771393370993 1101 26-27 -0.14264671505918614 1101 28-29 -5.027069951069052E-5 1101 30-31 0.6510932865794814 1101 32-33 0.5185993777309008 1101 34-35 0.7948560393327782 1101 36-37 0.385604616636833 1101 38-39 0.9896721532491242 1101 40-41 0.6517835974986568 1101 42-43 0.3159683307259158 1101 44-45 1.1501536847252467 1101 46-47 1.2402526673691412 1101 48-49 0.7000602328976377 1101 50-51 0.1414475692443382 1101 52-53 0.18360586425236392 1101 54-55 -0.31423750360641023 1101 56-57 -0.3216042976846687 1101 58-59 -0.3647553974465332 1101 60-61 0.08291288305190747 1101 62-63 -0.44519403839631977 1101 64-65 -0.2095626353159865 1101 66-67 -0.04079874765502112 1101 68-69 0.6808744187690543 1101 70-71 0.43010672021828 1101 72-73 -0.0960327303401236 1101 74-75 -0.2764189323867008 1101 76-77 -0.3360815801108288 1101 78-79 0.015620655720365306 1101 80-81 -0.5572195869790022 1101 82-83 -0.7487691998979109 1101 84-85 -0.38784461166705597 1101 86-87 -0.7237845040390773 1101 88-89 -0.26890451603952314 1101 90-91 -0.21228754312991427 1101 92-93 -0.7264209052245576 1101 94-95 -0.5001854527349323 1101 96-97 -0.12040188656030182 1101 98-99 0.3385332165809096 1101 100-101 -0.1637294165221519 1102 1 0.3973637204114624 1102 2 0.12379220378062428 1102 3 0.1906936188718653 1102 4 -0.6122808810308698 1102 5 0.3066011730135685 1102 6 0.22731662864412527 1102 7 0.5340589849315833 1102 8 0.5974915635519338 1102 9 0.9167914157112307 1102 10-11 0.3240367859809723 1102 12-13 0.3885269106282081 1102 14-15 0.09696731540888237 1102 16-17 0.72735107139885 1102 18-19 0.7046611477157185 1102 20-21 0.7234756483533928 1102 22-23 0.7023402407674126 1102 24-25 0.7330714616935268 1102 26-27 0.48351815949278887 1102 28-29 0.26141926335067467 1102 30-31 0.12779579554005238 1102 32-33 0.47021228095670864 1102 34-35 0.7081176880782962 1102 36-37 0.6020920718339724 1102 38-39 0.7059803969767273 1102 40-41 0.636012988179651 1102 42-43 0.4027066819803977 1102 44-45 0.37040458078259775 1102 46-47 0.5794999791971023 1102 48-49 0.9488057526108982 1102 50-51 0.6769314402120798 1102 52-53 0.9375485165821118 1102 54-55 1.0518915286516552 1102 56-57 0.057965594788452535 1102 58-59 -0.16995253006302136 1102 60-61 0.24026055330280016 1102 62-63 1.07559449206963 1102 64-65 1.2700072571571326 1102 66-67 1.2170865570044782 1102 68-69 1.8477202969052549 1102 70-71 1.6724006270283205 1102 72-73 2.1919601012010936 1102 74-75 2.5692799923444802 1102 76-77 2.3619470937242966 1102 78-79 2.362753272207822 1102 80-81 2.315540269651823 1102 82-83 1.6001555312848836 1102 84-85 2.8843776105551697 1102 86-87 2.9149251733802792 1102 88-89 2.491848172132517 1102 90-91 2.240221417443564 1102 92-93 1.5888120696858365 1102 94-95 1.7021442963690099 1102 96-97 1.8371249951601314 1102 98-99 0.4596801699859263 1102 100-101 0.6640483612556238 1103 1 0.023879391882520196 1103 2 0.042346310392382236 1103 3 0.0994101597092012 1103 4 -0.10747648844336055 1103 5 -0.35834963915755935 1103 6 -0.3766641537855264 1103 7 0.02296312586042859 1103 8 -0.1268278141648338 1103 9 -0.2300857291068894 1103 10-11 0.18493818154262698 1103 12-13 -0.14241566544134088 1103 14-15 0.4611860296619099 1103 16-17 0.13761188128216872 1103 18-19 -0.3126117875393746 1103 20-21 -0.12592952208814978 1103 22-23 0.3066641940961716 1103 24-25 0.4667708439859126 1103 26-27 0.621301275484555 1103 28-29 0.5168528025865484 1103 30-31 0.3295802842154103 1103 32-33 0.17108164723444474 1103 34-35 -0.14225979991072535 1103 36-37 0.23678664973376584 1103 38-39 0.3069298364642492 1103 40-41 0.49268194265231813 1103 42-43 0.43748133308998405 1103 44-45 -0.007884140333857204 1103 46-47 0.31365692268829193 1103 48-49 -0.3937931949983451 1103 50-51 -0.3578432108975065 1103 52-53 0.031005391439123287 1103 54-55 -0.1557268653085373 1103 56-57 0.3156297448685237 1103 58-59 0.25746682477568683 1103 60-61 -0.24321004839017846 1103 62-63 -0.5294729982071935 1103 64-65 -1.1354491601386805 1103 66-67 -1.0339429556928472 1103 68-69 -0.9004494582995015 1103 70-71 -0.9896332325003954 1103 72-73 -1.3278294206474506 1103 74-75 -1.0136529749398981 1103 76-77 -2.011022478455981 1103 78-79 -1.6594384459344802 1103 80-81 -1.423649847026283 1103 82-83 -0.8913223913872734 1103 84-85 -0.4542748717143361 1103 86-87 0.15245663071269533 1103 88-89 0.03428697332217823 1103 90-91 0.31371718500247425 1103 92-93 0.6114041538770962 1103 94-95 0.8287743581397677 1103 96-97 0.5168321559928906 1103 98-99 0.4433795522783086 1103 100-101 0.2544738931705197 1104 1 0.6310133092792682 1104 2 0.6325841076389409 1104 3 0.4218131634638951 1104 4 0.662861433959641 1104 5 0.542401299515781 1104 6 0.241358374374677 1104 7 0.11457764400811499 1104 8 0.2997053522932376 1104 9 0.34563392045506447 1104 10-11 0.5075914981884395 1104 12-13 0.4211012306788078 1104 14-15 0.6041146904879398 1104 16-17 0.521278965136986 1104 18-19 0.6633581749136894 1104 20-21 0.9382131562597849 1104 22-23 0.6499683242588716 1104 24-25 0.8949310442362233 1104 26-27 0.7704251803656561 1104 28-29 0.2486425397580092 1104 30-31 0.15436126043569232 1104 32-33 0.19310918165247415 1104 34-35 0.2549867582870249 1104 36-37 -0.6307352526442074 1104 38-39 -0.9577134676659114 1104 40-41 -0.5194582325667056 1104 42-43 -0.12847611371852707 1104 44-45 -0.7828528512224651 1104 46-47 -0.33327674439556176 1104 48-49 -0.6202012050108578 1104 50-51 -1.3212975287948794 1104 52-53 -1.5010346586234533 1104 54-55 -0.5995316212534654 1104 56-57 -1.1510160623905499 1104 58-59 -1.3925331752243117 1104 60-61 -2.2530348293663955 1104 62-63 -2.5831025351283436 1104 64-65 -2.442895968649321 1104 66-67 -2.5502758718380285 1104 68-69 -1.9684719864597042 1104 70-71 -1.6058409922000187 1104 72-73 -1.4388431878564667 1104 74-75 -1.3028895206219993 1104 76-77 -1.8089573971042903 1104 78-79 -1.9585623508155798 1104 80-81 -1.3742130510312904 1104 82-83 -1.028180964603795 1104 84-85 -2.572485134542873 1104 86-87 -2.08652960207829 1104 88-89 -2.0659633395689347 1104 90-91 -2.4452940790776267 1104 92-93 -1.6875320163356662 1104 94-95 -1.2652181324734961 1104 96-97 -1.2174607100897106 1104 98-99 -1.0314014239419684 1104 100-101 -0.6947751681561414 1105 1 -0.08094967816290222 1105 2 0.6251815960725153 1105 3 0.5201608171321048 1105 4 0.6258488761275203 1105 5 0.9248203345455224 1105 6 0.5767185858750068 1105 7 0.007902164827676472 1105 8 0.3316947772767165 1105 9 0.5822499151675586 1105 10-11 0.5296668451811612 1105 12-13 0.6877899286299041 1105 14-15 0.5301556686769757 1105 16-17 0.7525413577344793 1105 18-19 0.9068479303664319 1105 20-21 0.6097267054997033 1105 22-23 0.46946601097400986 1105 24-25 -0.20368098484507158 1105 26-27 0.2385018492354476 1105 28-29 -0.09299658780312825 1105 30-31 -0.0898555274030457 1105 32-33 0.5541799020754752 1105 34-35 0.5366787609307764 1105 36-37 1.2296745292460756 1105 38-39 1.206463663860859 1105 40-41 0.07300706816032942 1105 42-43 -0.9127457766398805 1105 44-45 -1.9977239682085859 1105 46-47 -1.4097473326308574 1105 48-49 -2.086955996815224 1105 50-51 -2.4289644157743915 1105 52-53 -3.1145838985441436 1105 54-55 -3.4136757058536027 1105 56-57 -3.671042499931861 1105 58-59 -4.110510703314201 1105 60-61 -4.823953533926872 1105 62-63 -4.929963077097941 1105 64-65 -4.477727429323476 1105 66-67 -4.183058423060764 1105 68-69 -3.9072029844768856 1105 70-71 -3.895860820355999 1105 72-73 -3.6570189975061673 1105 74-75 -3.1870930896900767 1105 76-77 -3.382585949648906 1105 78-79 -3.1727064354157157 1105 80-81 -3.2526003610114635 1105 82-83 -3.171802907763084 1105 84-85 -3.196146734014121 1105 86-87 -3.004308848608364 1105 88-89 -2.917780920533904 1105 90-91 -2.9073562072996992 1105 92-93 -3.5852848253244325 1105 94-95 -3.4788995601007286 1105 96-97 -4.24792997644794 1105 98-99 -4.797296995653797 1105 100-101 -4.9764010769466225 1106 1 0.027932076786267857 1106 2 0.24168774909552582 1106 3 -0.03280868527560088 1106 4 0.16076059362350748 1106 5 0.12265340035611416 1106 6 0.024551651685598586 1106 7 0.03866727986245877 1106 8 0.27337482008034897 1106 9 0.2651017075699116 1106 10-11 0.41543463544334003 1106 12-13 0.27159142675724723 1106 14-15 0.3097169313843011 1106 16-17 0.577581486145391 1106 18-19 0.5691004718324635 1106 20-21 0.0222467697051556 1106 22-23 -0.5388271939484142 1106 24-25 -0.22775803139403195 1106 26-27 -0.5767736991861625 1106 28-29 -0.4123518580010952 1106 30-31 -0.44612893564274003 1106 32-33 0.030504139635667116 1106 34-35 -0.5190328495561118 1106 36-37 0.22171572774794157 1106 38-39 -0.007153243576269119 1106 40-41 0.5676566133716676 1106 42-43 0.8322381719957619 1106 44-45 1.0910267005982561 1106 46-47 0.8700145721310477 1106 48-49 1.4052189630563667 1106 50-51 0.7176380454348141 1106 52-53 1.1451138007602992 1106 54-55 0.2202863059174014 1106 56-57 0.5105385594581904 1106 58-59 0.7634192057280664 1106 60-61 1.2761668513058737 1106 62-63 0.1813932631909836 1106 64-65 0.5856754599221112 1106 66-67 0.4238837920275209 1106 68-69 0.4780966409912786 1106 70-71 -0.09449645438489185 1106 72-73 0.14722123791384334 1106 74-75 0.4164382104704387 1106 76-77 0.1420930230637758 1106 78-79 0.18744605254576285 1106 80-81 0.2661931114336973 1106 82-83 0.9964688953401826 1106 84-85 1.6403299915075493 1106 86-87 2.088120257865686 1106 88-89 2.623952626817619 1106 90-91 2.785728329885959 1106 92-93 2.7958013169976645 1106 94-95 2.6589415313920526 1106 96-97 2.241502875344459 1106 98-99 1.8619459149936084 1106 100-101 1.8783340755413391 1107 1 0.39210956596375723 1107 2 0.3769691343769104 1107 3 0.6214120939451746 1107 4 0.27115020401311796 1107 5 0.6080430107457246 1107 6 0.4303958075297558 1107 7 0.7865027776979545 1107 8 0.3637427854483164 1107 9 1.1217034391716396 1107 10-11 0.9419497869584887 1107 12-13 0.9458338509996693 1107 14-15 1.2255719097392799 1107 16-17 0.5361853822492861 1107 18-19 0.2741870519190357 1107 20-21 0.6293896268480168 1107 22-23 0.5428827627615433 1107 24-25 0.47922248808649215 1107 26-27 0.7249037899913304 1107 28-29 1.2437628606136215 1107 30-31 1.1296286401148308 1107 32-33 0.8746599837915028 1107 34-35 0.9601338171105525 1107 36-37 1.3145187580509727 1107 38-39 1.2861367997137734 1107 40-41 1.7367583449734028 1107 42-43 1.5351602499178298 1107 44-45 1.555582977654538 1107 46-47 0.9084344855509556 1107 48-49 0.6138228591602619 1107 50-51 1.0501596904564572 1107 52-53 1.677040207686705 1107 54-55 1.9675806782117746 1107 56-57 2.232941156860786 1107 58-59 1.8597395520484135 1107 60-61 2.1349330850721095 1107 62-63 2.443568587866306 1107 64-65 2.492331304077954 1107 66-67 2.415766908910637 1107 68-69 2.7015814894761228 1107 70-71 2.6230360131475727 1107 72-73 2.485154138346747 1107 74-75 3.0463083403405697 1107 76-77 3.4334891269598806 1107 78-79 2.4074135850132983 1107 80-81 2.6555329382735238 1107 82-83 2.4410035273748143 1107 84-85 1.9146806408581973 1107 86-87 2.0440185262639545 1107 88-89 2.1629136657786603 1107 90-91 2.4783690225266497 1107 92-93 2.6670134382097856 1107 94-95 2.3780973755478954 1107 96-97 2.3489162952578795 1107 98-99 2.9501493781970716 1107 100-101 2.874275633982897 1108 1 0.3386173264959069 1108 2 -0.10529250642131771 1108 3 0.29297528684983476 1108 4 0.5100637295120123 1108 5 0.7158589752690077 1108 6 0.40434259245215287 1108 7 0.7715360371214572 1108 8 0.5236651801268124 1108 9 0.46399833939337043 1108 10-11 0.4499874809717923 1108 12-13 0.4677296381393532 1108 14-15 0.19328255275480188 1108 16-17 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-1.4014291527087543 2316 80-81 -1.4556870199291936 2316 82-83 -0.5412979355300962 2316 84-85 -1.1041281365712692 2316 86-87 -0.8375776074873507 2316 88-89 -1.2487895735107877 2316 90-91 -1.477613213627997 2316 92-93 -1.7070722462207257 2316 94-95 -1.0498023109724812 2316 96-97 -1.6209765992039777 2316 98-99 -1.8082100784382469 2316 100-101 -2.5773309490892036 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 273.0 3 82.0 4 56.0 5 66.0 6 78.0 7 98.0 8 123.0 9 158.0 10 241.0 11 333.0 12 495.0 13 771.0 14 940.0 15 1320.0 16 1718.0 17 2364.0 18 2090.0 19 1399.0 20 1451.0 21 1433.0 22 1482.0 23 1596.0 24 1730.0 25 1825.0 26 1967.0 27 2234.0 28 2510.0 29 2820.0 30 3205.0 31 3633.0 32 3997.0 33 4852.0 34 7174.0 35 15538.0 36 8173.0 37 3408.0 38 2984.0 39 624.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.14234750158424 21.632595582885443 16.986645386908254 6.238411528622057 2 15.096619774495196 30.180333446750595 41.21153101571024 13.511515763043963 3 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37.94865177173091 7.242364341312624 48-49 6.256342274596294 48.97673081773549 37.45300117900319 7.313925728665029 50-51 6.6012059782736205 49.3753079467868 36.798174608760945 7.225311466178644 52-53 5.754792238567842 49.32662302620774 37.02576194833533 7.892822786889092 54-55 5.497419052088222 48.86262318160488 36.91224777099953 8.727709995307368 56-57 5.774148893737829 48.94300931465709 36.38640576241759 8.89643602918749 58-59 5.929797393212027 47.913513767172304 36.81765389082462 9.339034948791046 60-61 5.908128369222828 47.391143673340096 37.40973855710741 9.290989400329664 62-63 6.820274875789696 47.19639599476762 37.37982249804959 8.603506631393092 64-65 6.530933065854846 46.97063015819945 38.15146555921188 8.346971216733829 66-67 5.926008495060193 46.95632788116303 38.370332996972756 8.747330626804027 68-69 5.848323769962803 47.197873763508994 38.355569636591916 8.598232829936284 70-71 6.004811359502435 46.397934635920905 38.095992489585164 9.501261514991493 72-73 5.965254023480817 45.867387949799635 38.170115643903614 9.997242382815937 74-75 6.282599639753812 45.37459149607778 38.63317667904646 9.709632185121949 76-77 6.549344537420635 44.87600783973148 38.67992066379523 9.89472695905266 78-79 6.259754280148794 44.51354744833898 38.763069268590336 10.463629002921884 80-81 6.270102124662519 45.05810541143327 38.191689165395 10.480103298509215 82-83 6.419848716910492 44.495889301621396 37.64765945460627 11.436602526861844 84-85 6.8605190126672895 43.55165191065426 37.49141921743263 12.09640985924583 86-87 7.472791809674676 43.11654786001349 37.50770043122415 11.902959899087682 88-89 8.126580347661818 41.92768681102709 37.67783494570351 12.267897895607586 90-91 7.938882923480435 41.80792949473968 38.01803704812031 12.235150533659573 92-93 8.229660579315524 41.661776442420546 37.453053847237214 12.655509131026713 94-95 8.70429905664805 41.15666212981649 36.95792462570986 13.181114187825596 96-97 9.072454074478259 40.391577045159856 36.53858565234483 13.997383228017062 98-99 9.790332897138128 39.945309336729004 36.469635532500455 13.794722233632411 100-101 10.690454532116552 39.74823204906247 36.17007541301094 13.39123800581003 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3373.0 1 2292.0 2 1284.0 3 1311.0 4 1117.5 5 1247.0 6 2455.0 7 4866.0 8 6769.5 9 6634.5 10 4744.5 11 3256.0 12 3109.5 13 2957.5 14 2449.5 15 1768.5 16 1240.5 17 970.0 18 835.5 19 720.0 20 629.5 21 624.0 22 637.0 23 626.0 24 656.0 25 693.0 26 754.5 27 836.5 28 884.0 29 1000.5 30 1142.0 31 1183.5 32 1229.5 33 1317.5 34 1396.0 35 1431.0 36 1425.5 37 1343.0 38 1226.5 39 1151.0 40 1060.0 41 929.0 42 803.5 43 706.5 44 637.0 45 577.5 46 643.0 47 816.0 48 1688.0 49 1680.5 50 829.5 51 623.0 52 349.0 53 243.5 54 215.5 55 190.5 56 197.0 57 180.5 58 147.5 59 129.5 60 134.5 61 143.5 62 115.0 63 61.5 64 26.5 65 24.5 66 26.0 67 19.0 68 19.0 69 17.5 70 14.5 71 12.0 72 11.5 73 23.0 74 24.0 75 10.5 76 5.5 77 3.0 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 1.0 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03284765725815912 2 0.012904436779991084 3 0.01642382862907956 4 0.029328265409070648 5 0.0633490532835926 6 0.05513713896905281 7 0.021116351094530864 8 0.06217592266722977 9 0.0363670491072476 10-11 0.027568569484526406 12-13 0.0451655287299688 14-15 0.021116351094530864 16-17 0.021116351094530864 18-19 0.02404917763543793 20-21 0.018770089861805214 22-23 0.009385044930902607 24-25 0.0011731306163628259 26-27 0.0 28-29 0.00410595715726989 30-31 0.0 32-33 0.0011731306163628259 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 5.865653081814129E-4 46-47 5.865653081814129E-4 48-49 5.865653081814129E-4 50-51 0.0 52-53 0.0 54-55 0.0023462612327256518 56-57 0.0 58-59 0.0035193918490884777 60-61 0.00410595715726989 62-63 0.00410595715726989 64-65 0.0017596959245442388 66-67 0.018770089861805214 68-69 0.02463574294361934 70-71 0.0316745266417963 72-73 0.027568569484526406 74-75 0.026395438868163583 76-77 0.041059571572698905 78-79 0.026982004176344995 80-81 0.06100279205086695 82-83 0.043992398113605974 84-85 0.026395438868163583 86-87 0.022876047019075106 88-89 0.0181835245536238 90-91 0.033434222566340537 92-93 0.04457896342178738 94-95 0.01642382862907956 96-97 0.026395438868163583 98-99 0.04164613688088032 100-101 0.05220431242814576 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 85242.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.374768306703274 #Duplication Level Percentage of deduplicated Percentage of total 1 82.77095954047522 30.935454353487717 2 9.363131297278635 6.998897257220619 3 2.909695847327286 3.2624762441050184 4 1.2806428324806176 1.9145491659041318 5 0.7439028218085941 1.3901597803899486 6 0.5367400106720236 1.2036320123882593 7 0.3484101823660504 0.9115224889139156 8 0.21030164160833675 0.6287980103704747 9 0.21030164160833675 0.7073977616667839 >10 1.355974763803007 9.854297177447737 >50 0.1255532188706488 3.3246521667722484 >100 0.11613672745535014 7.603059524647474 >500 0.00941649141529866 2.4236878534055983 >1k 0.01255532188706488 14.573801647075385 >5k 0.00627766094353244 14.267614556204688 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6150 7.2147532906313785 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6012 7.052861265573308 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4508 5.288472818563618 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3543 4.156401773773491 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3322 3.897139907557307 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1050 1.2317871471809672 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 774 0.9080030970648271 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 732 0.8587316111775884 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 560 0.6569531451631825 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 494 0.5795265244832359 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 392 0.4598672016142277 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 360 0.4223270218906173 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 302 0.3542854461415734 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 273 0.32026465826705147 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 271 0.3179183970343258 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 207 0.24283803758710495 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 207 0.24283803758710495 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 200 0.23462612327256518 No Hit GTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 199 0.23345299265620234 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 197 0.2311067314234767 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 196 0.22993360080711386 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 184 0.21585603341075996 No Hit GTACATGGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 166 0.1947396823162291 No Hit ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 158 0.18535463738532648 No Hit GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 153 0.17948898430351234 No Hit TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 148 0.17362333122169823 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 142 0.16658454752352128 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 141 0.16541141690715844 No Hit GAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 141 0.16541141690715844 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 137 0.16071889444170712 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 130 0.15250698012716735 No Hit GTACATGGGGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAAA 130 0.15250698012716735 No Hit GTACATGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 122 0.14312193519626476 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 120 0.1407756739635391 No Hit GTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 118 0.13842941273081344 No Hit GTATCAACGCAGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 115 0.13491002088172496 No Hit GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 114 0.13373689026536215 No Hit GGTATCAACGCAGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 114 0.13373689026536215 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 114 0.13373689026536215 No Hit GAGTACATGGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 114 0.13373689026536215 No Hit GAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 110 0.12904436779991085 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 104 0.1220055841017339 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 103 0.12083245348537106 No Hit GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 103 0.12083245348537106 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 101 0.11848619225264541 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 101 0.11848619225264541 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 99 0.11613993101991975 No Hit GTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 96 0.11262053917083129 No Hit GAGTACATGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 95 0.11144740855446844 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 94 0.11027427793810562 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 90 0.10558175547265433 No Hit GTATCAACGCAGAGTACATGGGATGAAAAAAAAAAAAAAAAAAAAAAAAA 89 0.10440862485629152 No Hit ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 87 0.10206236362356585 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 86 0.10088923300720302 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0023462612327256518 0.0 18-19 0.0 0.0 0.0 0.0035193918490884777 0.0 20-21 0.0 0.0 0.0 0.0058656530818141294 0.0 22-23 0.0 0.0 0.0 0.00821191431453978 0.0 24-25 0.0 0.0 0.0 0.022876047019075103 0.0 26-27 0.0 0.0 0.0 0.03284765725815912 0.0 28-29 0.0 0.0 0.0 0.04927148588723869 0.0 30-31 0.0 0.0 0.0 0.07566692475540227 0.0 32-33 0.0 0.0 0.0 0.09795640646629596 0.0 34-35 0.0 0.0 0.0 0.11965932286900824 0.0 36-37 0.0 0.0 0.0 0.14781445766171605 0.0 38-39 0.0 0.0 0.0 0.16951737406442835 0.0 40-41 0.0 0.0 0.0 0.19122029046714062 0.0 42-43 0.0 0.0 0.0 0.2487036906689191 0.0 44-45 0.0 0.0 0.0 0.3120527439525117 0.0 46-47 0.0 0.0 0.0 0.3531123155252106 0.0 48-49 0.0 0.0 0.0 0.40942258511062624 0.0 50-51 0.0 0.0 0.0 0.4680791159287675 0.0 52-53 0.0 0.0 0.0 0.5443326059923512 0.0 54-55 0.0 0.0 0.0 0.5859787428732315 0.0 56-57 0.0 0.0 0.0 0.6610591023204524 0.0 58-59 0.0 0.0 0.0 0.7842378170385491 0.0 60-61 0.0 0.0 0.0 0.8728091785739425 0.0 62-63 0.0 0.0 0.0 0.9783909340465968 0.0 64-65 0.0 0.0 0.0 1.0816264282865253 0.0 66-67 0.0 0.0 0.0 1.2006991858473524 0.0 68-69 0.0 0.0 0.0 1.3385420332699844 0.0 70-71 0.0 0.0 0.0 1.4470566152835458 0.0 72-73 0.0 0.0 0.0 1.6118814668825228 0.0 74-75 0.0 0.0 0.0 1.7438586612233407 0.0 76-77 0.0 0.0 0.0 1.8236315431360128 0.0 78-79 0.0 0.0 0.0 1.8969522066586895 0.0 80-81 0.0 0.0 0.0 2.0389010112385915 0.0 82-83 0.0 0.0 0.0 2.1480021585603337 0.0 84-85 0.0 0.0 0.0 2.332770230637479 0.0 86-87 0.0 0.0 0.0 2.608455925482743 0.0 88-89 0.0 0.0 0.0 2.866544661082565 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACAG 20 0.002067721 71.247795 5 GGACTGA 20 0.002067721 71.247795 8 GACTGAA 20 0.0020725452 71.205986 9 GTATGGG 25 0.0050176326 56.964787 1 TATGGGA 60 2.3173925E-8 55.414955 4 GTATATG 95 0.0 54.966026 1 TATATGG 100 0.0 52.248383 2 ATATGGG 100 0.0 52.248383 3 GGTATCA 1705 0.0 45.104084 1 GGGGGAT 45 9.576325E-4 42.22092 7 GAAGGGA 45 9.604063E-4 42.19614 9 GGGAAGG 60 8.227708E-5 39.58211 7 GTATCAA 2930 0.0 39.369865 1 GGGAACT 50 0.0016076694 37.998825 7 TGGGATA 50 0.0016076694 37.998825 6 GAGTAAT 50 0.0016123179 37.976524 1 CGCAGAA 100 2.9909643E-8 37.976524 9 ATGGGAC 65 1.3181222E-4 36.53733 5 TGGGACT 65 1.3181222E-4 36.53733 6 AGTAATG 55 0.0025666782 34.544388 2 >>END_MODULE