##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936877_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 85242 Sequences flagged as poor quality 0 Sequence length 101 %GC 19 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.91256657516248 31.0 31.0 34.0 30.0 34.0 2 31.857077497008518 31.0 31.0 34.0 30.0 34.0 3 31.798714248844465 31.0 31.0 34.0 30.0 34.0 4 35.710354050820015 37.0 35.0 37.0 33.0 37.0 5 35.69008235376927 37.0 35.0 37.0 33.0 37.0 6 35.88091551113301 37.0 35.0 37.0 35.0 37.0 7 35.81168907346144 37.0 35.0 37.0 35.0 37.0 8 35.846660097135214 37.0 35.0 37.0 35.0 37.0 9 37.294831186504304 39.0 37.0 39.0 34.0 39.0 10-11 37.25927946317543 39.0 37.0 39.0 34.0 39.0 12-13 37.03723516576336 39.0 37.0 39.0 32.5 39.0 14-15 37.68464489336243 39.5 37.0 41.0 32.0 41.0 16-17 37.67406912085592 39.0 36.5 40.5 32.5 41.0 18-19 37.749225733793196 39.0 35.5 41.0 33.0 41.0 20-21 37.92041481898595 40.0 35.0 41.0 33.5 41.0 22-23 37.681471575045165 40.0 35.0 41.0 33.0 41.0 24-25 37.462412895051735 39.0 35.0 41.0 33.0 41.0 26-27 37.042361746556864 38.5 35.0 41.0 33.0 41.0 28-29 36.50311466178644 37.5 35.0 40.5 32.5 41.0 30-31 35.53197367494897 35.5 35.0 39.5 31.5 41.0 32-33 34.53627319865794 35.0 34.5 37.5 30.5 41.0 34-35 34.01009478895381 35.0 33.0 36.5 30.0 40.5 36-37 33.444235236151194 35.0 33.0 35.0 25.0 40.0 38-39 32.96806151896952 35.0 33.0 35.0 23.5 40.0 40-41 32.44868726684029 35.0 33.0 35.0 21.5 40.0 42-43 31.808767978226697 35.0 33.0 35.0 19.5 40.0 44-45 31.06101452335703 35.0 33.0 35.0 15.5 40.0 46-47 30.050544332605995 35.0 31.0 35.0 3.5 40.0 48-49 29.251665845475237 35.0 27.5 35.0 2.0 39.0 50-51 28.884511156472165 35.0 27.0 35.0 2.0 39.0 52-53 28.647480115436053 35.0 27.0 35.0 2.0 39.0 54-55 28.36543018699702 35.0 27.0 35.0 2.0 38.0 56-57 27.995483447127 35.0 24.5 35.0 2.0 38.0 58-59 27.60370474648647 34.5 24.5 35.0 2.0 38.0 60-61 27.26914549165904 33.5 24.0 35.0 2.0 38.0 62-63 26.81161868562446 33.0 22.0 35.0 2.0 37.0 64-65 26.38318551887567 33.0 21.0 35.0 2.0 36.0 66-67 26.025720888763757 33.0 20.0 35.0 2.0 36.0 68-69 25.907510382205956 33.0 20.0 35.0 2.0 36.0 70-71 25.52787944909786 33.0 14.5 35.0 2.0 35.0 72-73 25.0155146524014 33.0 3.5 35.0 2.0 35.0 74-75 24.482502756856945 33.0 2.0 35.0 2.0 35.0 76-77 23.030929588700406 30.0 2.0 33.5 2.0 35.0 78-79 23.622275404143497 33.0 2.0 35.0 2.0 35.0 80-81 23.24984749301987 33.0 2.0 35.0 2.0 35.0 82-83 22.736802280565918 33.0 2.0 35.0 2.0 35.0 84-85 22.142541235541167 31.5 2.0 35.0 2.0 35.0 86-87 21.486925459280634 31.0 2.0 35.0 2.0 35.0 88-89 20.720196616691304 30.5 2.0 35.0 2.0 35.0 90-91 19.881942000422328 29.0 2.0 35.0 2.0 35.0 92-93 19.026049365336338 27.0 2.0 34.0 2.0 35.0 94-95 18.096906454564653 24.0 2.0 34.0 2.0 35.0 96-97 17.156929682550853 11.0 2.0 33.0 2.0 35.0 98-99 16.228261889678798 2.0 2.0 33.0 2.0 35.0 100-101 13.760511250322612 2.0 2.0 29.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.387472931625517 1101 2 -0.13600883675279363 1101 3 -0.5314642688313249 1101 4 -0.2889181043868305 1101 5 -0.02942582702638674 1101 6 -0.08898942691940448 1101 7 -0.25199918823123824 1101 8 -0.2699072442773698 1101 9 0.1346595765866283 1101 10-11 -0.29526699982502436 1101 12-13 -0.1117092557258843 1101 14-15 -0.13732806836833333 1101 16-17 -0.5511041932021286 1101 18-19 0.2865287760250652 1101 20-21 0.493758842612543 1101 22-23 0.6701813784805779 1101 24-25 0.9104521821668499 1101 26-27 0.7077056614415156 1101 28-29 0.911593589344875 1101 30-31 0.877949596614819 1101 32-33 0.7496759576499272 1101 34-35 0.5613367027309906 1101 36-37 0.3217790830996776 1101 38-39 0.5651122732220983 1101 40-41 0.8490367471842362 1101 42-43 0.8515961216311752 1101 44-45 1.562618302448712 1101 46-47 0.840016154149513 1101 48-49 0.9384188224512862 1101 50-51 0.09267280731229022 1101 52-53 1.1396575978038577 1101 54-55 0.7828359964744322 1101 56-57 0.5701581523470729 1101 58-59 0.7240461381733887 1101 60-61 1.7560203620644437 1101 62-63 2.1876525719485365 1101 64-65 1.9802408812272354 1101 66-67 1.6262173490680958 1101 68-69 1.4329494926710566 1101 70-71 1.5381883585521763 1101 72-73 1.5368909265577209 1101 74-75 1.2873959098812797 1101 76-77 1.0112701863868416 1101 78-79 0.33391858543440023 1101 80-81 0.7419034199602379 1101 82-83 1.4586290358618612 1101 84-85 1.3574226595525083 1101 86-87 0.09164482840820654 1101 88-89 0.5390263116202902 1101 90-91 0.48198925611234245 1101 92-93 -0.30490336533264895 1101 94-95 -0.1824297607343972 1101 96-97 0.3678073767613128 1101 98-99 -0.8526099705180599 1101 100-101 -0.24397382303799375 1102 1 0.467007355112834 1102 2 0.22743202346225644 1102 3 0.697567989233189 1102 4 0.3117987414913017 1102 5 0.3365239937621425 1102 6 0.30455896017737416 1102 7 0.24656712001249304 1102 8 0.5186232216724491 1102 9 0.5185305443285699 1102 10-11 0.2543745772359145 1102 12-13 0.24635526040314915 1102 14-15 0.22682963772485465 1102 16-17 0.3103653408480511 1102 18-19 0.08617035308599696 1102 20-21 0.2806764053365569 1102 22-23 0.5142673999859468 1102 24-25 0.18894680582276635 1102 26-27 -0.07903268981296208 1102 28-29 -0.03625587302071409 1102 30-31 0.16164135288722292 1102 32-33 0.15612757055315285 1102 34-35 -0.07952351232277266 1102 36-37 -0.5296546086565925 1102 38-39 -0.4608733898603319 1102 40-41 -0.8538306363283077 1102 42-43 -1.5684755629566425 1102 44-45 -1.5497472889491384 1102 46-47 -1.237940835097799 1102 48-49 -1.1873876291616163 1102 50-51 -1.1514132141930844 1102 52-53 -0.9411667749559989 1102 54-55 -1.1022894515542419 1102 56-57 -0.6353974032084828 1102 58-59 -0.36538038512410154 1102 60-61 -0.9269545483298174 1102 62-63 -0.6114513707037936 1102 64-65 -0.4068558929663162 1102 66-67 -0.28005505236559713 1102 68-69 -0.15522255033969756 1102 70-71 0.2217009033550461 1102 72-73 0.5055289193892634 1102 74-75 0.27485110701389814 1102 76-77 -0.3435685232905783 1102 78-79 -0.017335894852340772 1102 80-81 0.35857008662690504 1102 82-83 0.6159767061127539 1102 84-85 0.6841251685130842 1102 86-87 1.0319674090533688 1102 88-89 1.6831123331256634 1102 90-91 1.3785842381911984 1102 92-93 1.2897202905813288 1102 94-95 0.3369250779752839 1102 96-97 0.14755648070396532 1102 98-99 0.7848452266145571 1102 100-101 0.7302197253491034 1103 1 -0.047520212949397234 1103 2 0.088104638424511 1103 3 -0.20096781493061755 1103 4 0.04607007528219498 1103 5 0.08097606422656867 1103 6 0.10037227520825809 1103 7 0.21419466519665775 1103 8 0.3341116682521772 1103 9 0.22260283899797884 1103 10-11 0.025536782680894987 1103 12-13 0.12658861661454068 1103 14-15 0.17461046591062512 1103 16-17 -0.18420111991607513 1103 18-19 0.5186800762614681 1103 20-21 0.1399763367023823 1103 22-23 0.5815288962110756 1103 24-25 0.3895301963512594 1103 26-27 0.6397387583682317 1103 28-29 0.8633666389902643 1103 30-31 1.122748650988342 1103 32-33 0.47910858176339843 1103 34-35 0.2939608162061731 1103 36-37 -0.04961571595469394 1103 38-39 -0.04008867240438008 1103 40-41 0.14170814198329396 1103 42-43 0.9623526228131993 1103 44-45 1.558008373370697 1103 46-47 0.45703743074525605 1103 48-49 0.06856066642291836 1103 50-51 0.37352387114207986 1103 52-53 0.605733247922057 1103 54-55 0.4911102281529196 1103 56-57 0.21212032728796615 1103 58-59 0.03693029892988875 1103 60-61 1.005783955445061 1103 62-63 0.403019002208584 1103 64-65 0.4500990372556011 1103 66-67 0.24453886207045983 1103 68-69 0.5825948827419793 1103 70-71 0.714665899923336 1103 72-73 0.6072218958248605 1103 74-75 0.3417694323399125 1103 76-77 0.021908484259181904 1103 78-79 -0.01950931054668814 1103 80-81 0.06424384549215745 1103 82-83 -0.36749389558022116 1103 84-85 0.4674699408763878 1103 86-87 0.38880794897558246 1103 88-89 0.7060475882160304 1103 90-91 0.08849043814544189 1103 92-93 -0.4158962731340665 1103 94-95 -0.22604678201099304 1103 96-97 0.022299102529636627 1103 98-99 0.6194940483944684 1103 100-101 0.7361680209336381 1104 1 0.33671007491042815 1104 2 0.3424225357962243 1104 3 0.3528494566588698 1104 4 0.2862452943059779 1104 5 0.10325391153570251 1104 6 -0.018401191625287083 1104 7 -0.09056127973450856 1104 8 0.20983131781413533 1104 9 -0.3222031685114075 1104 10-11 0.23773953612269594 1104 12-13 -0.34112102043176407 1104 14-15 0.4594039577754572 1104 16-17 -0.23182314745049837 1104 18-19 -0.0755627272429038 1104 20-21 0.19016407137071667 1104 22-23 -0.19844607249981294 1104 24-25 0.008491397853134686 1104 26-27 0.3501893215722305 1104 28-29 0.5047308442468363 1104 30-31 0.535139139098483 1104 32-33 0.46340144784601023 1104 34-35 0.7338857223388331 1104 36-37 -0.270704577031033 1104 38-39 -0.2659334784118883 1104 40-41 -1.1555384162144549 1104 42-43 -1.6470966888263447 1104 44-45 -1.9913032661787398 1104 46-47 -1.762925022321074 1104 48-49 -1.4635419618624397 1104 50-51 -2.2014448397465287 1104 52-53 -2.1149175655948405 1104 54-55 -1.6050725002575916 1104 56-57 -2.074613089482998 1104 58-59 -2.348176084048834 1104 60-61 -2.155417546432286 1104 62-63 -2.233589258116826 1104 64-65 -2.129563040341395 1104 66-67 -2.0799918012587 1104 68-69 -2.1827367818387486 1104 70-71 -1.8690011839314842 1104 72-73 -1.5647430603703825 1104 74-75 -1.9230616064585888 1104 76-77 -1.829906284201396 1104 78-79 -2.7804604995329214 1104 80-81 -2.266920109451526 1104 82-83 -2.553789264791739 1104 84-85 -2.2108779940422636 1104 86-87 -2.2603159559055186 1104 88-89 -1.9315619236738293 1104 90-91 -1.3078800249334073 1104 92-93 -1.7617661104306883 1104 94-95 -1.4824297607343944 1104 96-97 -1.3031076559184243 1104 98-99 -2.0052243496030258 1104 100-101 -1.716522842645837 1105 1 0.1463597774880867 1105 2 0.276725245644208 1105 3 -0.06816838868151365 1105 4 0.25465243244212843 1105 5 0.32625457247423384 1105 6 -0.10247257298681944 1105 7 0.1473765920434218 1105 8 0.12959337994235653 1105 9 0.7912138837027385 1105 10-11 0.3278915519852106 1105 12-13 0.595032317307826 1105 14-15 0.23395782426587886 1105 16-17 0.28741016385155405 1105 18-19 -0.4882527470710656 1105 20-21 -0.07996150695049664 1105 22-23 -0.0732643144033247 1105 24-25 0.2685046166237868 1105 26-27 0.22143849539907023 1105 28-29 -0.5110655866850848 1105 30-31 0.2529183856285542 1105 32-33 -0.3151180498407058 1105 34-35 -0.19109775419784825 1105 36-37 -0.7374593688354025 1105 38-39 -0.7385706231574218 1105 40-41 -0.2113877222289986 1105 42-43 -0.8110755387932969 1105 44-45 -1.0892543567273165 1105 46-47 -0.5223433964122854 1105 48-49 -1.9372366082603243 1105 50-51 -1.1612597769573725 1105 52-53 -1.2404297929576948 1105 54-55 -1.0193487725642676 1105 56-57 -0.8226633208114826 1105 58-59 -0.9301985559589454 1105 60-61 -1.6259396878731316 1105 62-63 -1.054607103457208 1105 64-65 -2.133991328510593 1105 66-67 -2.2715978818932 1105 68-69 -2.794765863134188 1105 70-71 -2.591274812484027 1105 72-73 -1.9915110709453998 1105 74-75 -1.4853881100937478 1105 76-77 -1.3932241956356286 1105 78-79 -1.5137718015818287 1105 80-81 -1.6227033216127928 1105 82-83 -1.9032935608921981 1105 84-85 -1.7432639821453684 1105 86-87 -2.8014263326404816 1105 88-89 -2.7227714411887014 1105 90-91 -2.1430419548739224 1105 92-93 -1.7854277099019384 1105 94-95 -2.4419803225321495 1105 96-97 -2.171580887907851 1105 98-99 -2.707603728320805 1105 100-101 -3.8454719503788173 1106 1 0.17284452869193956 1106 2 0.4282768775329231 1106 3 0.294726207359151 1106 4 0.4285422130734844 1106 5 0.4221614745609088 1106 6 0.539582001652029 1106 7 0.16784208161003278 1106 8 0.21025148588135778 1106 9 0.2965643384913932 1106 10-11 0.15433617477815176 1106 12-13 0.2561478871312559 1106 14-15 0.21108463004436828 1106 16-17 0.42230850521057306 1106 18-19 0.7123224268187158 1106 20-21 1.0132032870569887 1106 22-23 1.1761337594329575 1106 24-25 1.4664045631192408 1106 26-27 1.0390548677907248 1106 28-29 1.1056412083924911 1106 30-31 1.2438226124878398 1106 32-33 1.1949140528880235 1106 34-35 0.7899081313024183 1106 36-37 1.1356679719885676 1106 38-39 1.467890050999877 1106 40-41 1.4411002392477243 1106 42-43 1.8412786613137087 1106 44-45 1.3054754453058592 1106 46-47 -0.10046003632667677 1106 48-49 -0.5032478442153803 1106 50-51 -0.12875576411627776 1106 52-53 0.8662448993911589 1106 54-55 0.4748994885379254 1106 56-57 1.0967454539343713 1106 58-59 1.6661096302368819 1106 60-61 1.0659409969850806 1106 62-63 0.9094779687739312 1106 64-65 0.6290504050367574 1106 66-67 1.16867766652841 1106 68-69 1.2889018736234377 1106 70-71 0.6437439141077306 1106 72-73 -0.6019979623311649 1106 74-75 0.04136416384953279 1106 76-77 0.05293685305350593 1106 78-79 0.7426487441645584 1106 80-81 0.9597605628173831 1106 82-83 1.0447401469729698 1106 84-85 1.7232956754255255 1106 86-87 1.7952162569796357 1106 88-89 2.6723596449536196 1106 90-91 2.8597670338901224 1106 92-93 2.8903347299054474 1106 94-95 3.4651892868846517 1106 96-97 2.563839122793059 1106 98-99 2.0692154263073377 1106 100-101 2.426264272200102 1107 1 0.465052320899737 1107 2 0.36929419355023896 1107 3 0.17838421601715737 1107 4 0.117395026926296 1107 5 -0.07134501894557843 1107 6 0.17919239126241848 1107 7 0.4308290945970441 1107 8 0.03817356380343995 1107 9 0.2013262432532983 1107 10-11 0.1981673436093203 1107 12-13 0.4485180170013905 1107 14-15 0.295500214459949 1107 16-17 0.47452711992918495 1107 18-19 0.3273116043078872 1107 20-21 0.2707790446327536 1107 22-23 0.6209389542381487 1107 24-25 1.0153764245910963 1107 26-27 0.8377561664920208 1107 28-29 0.5453057105569954 1107 30-31 0.5852728289380522 1107 32-33 0.7890698970438663 1107 34-35 0.7495942784885656 1107 36-37 1.3042285780491696 1107 38-39 1.3258445964544237 1107 40-41 1.397269070416563 1107 42-43 1.3123284448634962 1107 44-45 0.6190576963881043 1107 46-47 0.8627434268767864 1107 48-49 1.451297610330073 1107 50-51 1.5676728073122916 1107 52-53 1.75720810285436 1107 54-55 1.5806895318279715 1107 56-57 0.8210419907309081 1107 58-59 1.1904602795875334 1107 60-61 1.3527375337816174 1107 62-63 0.8510364103323731 1107 64-65 1.1412257297120796 1107 66-67 1.8442729046236508 1107 68-69 2.273479795701359 1107 70-71 2.2479105807743984 1107 72-73 1.9283050679718627 1107 74-75 2.167446414931785 1107 76-77 2.8578610954777517 1107 78-79 3.0681357571515697 1107 80-81 3.0498579654147875 1107 82-83 2.379967419700243 1107 84-85 2.8867155888454405 1107 86-87 3.4897508890142674 1107 88-89 3.0606172207111975 1107 90-91 3.7631761247992124 1107 92-93 4.592445119515833 1107 94-95 4.707342966538331 1107 96-97 4.208590205044143 1107 98-99 3.099789019380932 1107 100-101 1.7723140557498844 1108 1 0.003854981653613976 1108 2 0.24041392747484736 1108 3 0.0644706905182666 1108 4 -0.09352515045729604 1108 5 -0.038639919167309245 1108 6 -0.010940646431599532 1108 7 0.10599539171456485 1108 8 -0.1547310925158527 1108 9 -0.07916156162475119 1108 10-11 0.304597498819966 1108 12-13 0.2321931832985129 1108 14-15 -0.32201641525180236 1108 16-17 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-0.8250525756222764 2316 58-59 -1.2953025208304432 2316 60-61 -0.8644777222267415 2316 62-63 -0.797213328434605 2316 64-65 -0.7841269348647195 2316 66-67 -0.8188018080200266 2316 68-69 -1.0245217716967616 2316 70-71 -1.3993595341681306 2316 72-73 0.035933072151589585 2316 74-75 -0.09574585257082546 2316 76-77 0.137706967996035 2316 78-79 0.42778831723516575 2316 80-81 0.668914914213115 2316 82-83 1.4628435952488346 2316 84-85 1.0541659545716655 2316 86-87 0.6899206904771731 2316 88-89 -0.31039897573603525 2316 90-91 -0.31552032243171624 2316 92-93 -1.8773171584360995 2316 94-95 -1.2088665423435891 2316 96-97 -1.7128439642348532 2316 98-99 -2.77540690538396 2316 100-101 -2.147422098900064 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 3.0 5 21.0 6 73.0 7 107.0 8 163.0 9 237.0 10 384.0 11 517.0 12 621.0 13 915.0 14 1174.0 15 1672.0 16 2203.0 17 2749.0 18 2468.0 19 1585.0 20 1541.0 21 1517.0 22 1549.0 23 1803.0 24 1916.0 25 2161.0 26 2407.0 27 2753.0 28 2899.0 29 3370.0 30 3699.0 31 4118.0 32 4703.0 33 5595.0 34 8129.0 35 12495.0 36 3792.0 37 3323.0 38 2355.0 39 221.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.966559892991654 20.494678916305865 23.14642073521302 6.392340455489458 2 14.442085010030855 29.084787122963036 43.02826237432102 13.444865492685102 3 17.25792449731353 38.53968700875156 37.499120152037726 6.703268341897187 4 8.162738588238055 24.673572576576998 38.04507220703652 29.118616628148423 5 5.594659909434316 44.51561436850379 32.44058093428122 17.44914478778067 6 14.99964806081509 42.000422327021894 22.685999859224324 20.31392975293869 7 14.601956781868095 47.8226695760306 23.128270101593113 14.447103540508198 8 26.641796297599775 29.745899908495808 23.00743764810774 20.604866145796674 9 29.36228619694517 16.947044883977384 24.512564228901248 29.178104690176205 10-11 21.51971511208368 33.81929329492587 24.64491678057881 20.01607481241163 12-13 28.263649374721382 35.8115717603998 29.149362990075318 6.7754158748035005 14-15 22.395063466366345 32.331479786959484 39.095750920907534 6.177705825766641 16-17 8.949813472231998 37.07737969545529 38.3238309753408 15.648975856971914 18-19 8.546356800131392 28.20884316232799 53.99866261540808 9.246137422132541 20-21 12.52023650313226 26.36259121090542 55.25034607353183 5.8668262124304915 22-23 10.170406217920517 29.069951605807304 55.19518991054407 5.564452265728113 24-25 8.479309171545564 30.479520362309486 55.980805106123356 5.0603653600215885 26-27 7.549947793850233 31.761282980795176 55.66524712866177 5.023522096692829 28-29 6.358405236885789 33.111805869206904 55.61962189778453 4.910166996122781 30-31 6.060641611478381 34.689723540418946 54.88189022566093 4.3677446224417364 32-33 5.7433978201954545 34.960756478993865 54.4170958621256 4.878749838685078 34-35 5.511954200980737 35.10241430280848 54.119448159358065 5.266183336852725 36-37 5.9613760090107 35.36406513985358 53.845386709217195 4.829172141918528 38-39 6.209044884167053 35.12181672054018 53.77887024011358 4.890268155179191 40-41 5.409337001343243 35.735527882545476 53.72265856419701 5.132476551914267 42-43 5.280847469557261 36.32774923159945 52.86654466108256 5.524858637760728 44-45 5.333638347293588 36.534220220079305 51.862931418784164 6.26921001384294 46-47 5.49618141504675 36.39621778252249 50.489201205992416 7.618399596438334 48-49 5.737430855775266 36.42485496577251 49.91289147509635 7.924822703355878 50-51 6.172419457024192 36.931858178661095 49.012131309102 7.883591055212709 52-53 5.3934680087280915 37.140142183430704 49.010464325097956 8.455925482743249 54-55 5.241793566249794 36.84330932213332 48.571646448766984 9.343250662849902 56-57 5.608869075551384 37.03542937587987 47.6237681839512 9.73193336461755 58-59 5.533689576086765 36.267545737698185 48.043499938410285 10.155264747804766 60-61 5.8076853139059486 35.83449182020284 48.36198755286512 9.995835313026085 62-63 6.562291023851144 35.87526543637153 48.16511608808381 9.397327451693513 64-65 6.244610899620491 35.95255831959785 48.89343805540729 8.909392725374378 66-67 5.763692141646283 35.96119215631067 48.95442893929528 9.320686762747755 68-69 5.590000175970625 36.132634925476445 48.955027774030256 9.32233712452268 70-71 5.614049494671023 35.73024876380636 48.331505194066274 10.324196547456346 72-73 5.714319232303325 35.12235752328664 48.120644752586756 11.04267849182328 74-75 5.966577312693935 34.7342550283606 48.520966899925504 10.778200759019962 76-77 5.8592008634241335 34.43255672086531 48.506604725370124 11.201637690340442 78-79 5.628259199080238 34.208905495691546 48.647047436927714 11.515787868300494 80-81 5.56259458698483 35.03853869707528 47.563908539518295 11.83495817642159 82-83 5.554154176468173 34.59320572310183 46.99912593053201 12.853514169897986 84-85 5.744085504279321 33.91779246910032 46.63197845967936 13.706143566941003 86-87 6.246040498369272 33.36774677960534 46.48044299490837 13.905769727117011 88-89 6.80365082530707 32.490233573045835 46.78910383500898 13.91701176663812 90-91 6.438765155470047 32.170949596147416 46.8926520532839 14.497633195098631 92-93 6.269246787069678 32.41965474563446 46.09843796742197 15.212660499873888 94-95 6.408812557190117 32.20712794162502 45.481100865770394 15.902958635414466 96-97 6.668817456593149 31.49108399812295 44.95248709526044 16.887611450023464 98-99 7.02717604894387 30.893178711997233 44.88066118805028 17.19898405100862 100-101 7.352596138054011 30.695549142441518 44.868726684029 17.083128035475468 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5813.0 1 3836.0 2 1966.0 3 1926.5 4 1442.0 5 1355.5 6 2501.0 7 4851.0 8 6543.5 9 6181.5 10 4427.0 11 2950.0 12 2534.0 13 2277.0 14 1874.0 15 1383.5 16 1037.5 17 890.5 18 745.5 19 638.5 20 585.5 21 542.5 22 576.5 23 605.5 24 609.0 25 646.0 26 707.0 27 767.0 28 847.5 29 972.5 30 1058.0 31 1080.0 32 1148.0 33 1260.5 34 1373.5 35 1385.0 36 1379.0 37 1357.0 38 1258.0 39 1188.0 40 1116.0 41 1008.5 42 867.0 43 777.0 44 719.0 45 663.5 46 697.5 47 820.0 48 1764.5 49 1724.0 50 832.5 51 658.5 52 351.5 53 216.5 54 183.0 55 170.5 56 173.0 57 164.5 58 130.5 59 101.5 60 84.0 61 88.0 62 79.5 63 44.5 64 24.0 65 17.5 66 15.0 67 16.5 68 17.0 69 11.0 70 10.0 71 14.5 72 14.0 73 14.5 74 10.5 75 5.5 76 6.0 77 6.5 78 4.5 79 3.0 80 2.0 81 0.5 82 0.5 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017596959245442387 2 0.005865653081814129 3 0.0 4 0.0011731306163628259 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0181835245536238 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0011731306163628259 20-21 0.0 22-23 0.005279087773632716 24-25 0.012904436779991084 26-27 0.0035193918490884777 28-29 5.865653081814129E-4 30-31 0.00410595715726989 32-33 0.005865653081814129 34-35 0.0 36-37 0.011731306163628257 38-39 0.013491002088172497 40-41 5.865653081814129E-4 42-43 0.0 44-45 0.0 46-47 0.0011731306163628259 48-49 0.00410595715726989 50-51 0.009385044930902607 52-53 0.0 54-55 0.0046925224654513036 56-57 0.0023462612327256518 58-59 5.865653081814129E-4 60-61 0.0017596959245442388 62-63 0.005865653081814129 64-65 5.865653081814129E-4 66-67 0.0017596959245442388 68-69 5.865653081814129E-4 70-71 5.865653081814129E-4 72-73 0.0 74-75 5.865653081814129E-4 76-77 0.0 78-79 0.0029328265409070643 80-81 0.0035193918490884777 82-83 0.00997161023908402 84-85 0.007625349006358369 86-87 0.0046925224654513036 88-89 0.0011731306163628259 90-91 5.865653081814129E-4 92-93 5.865653081814129E-4 94-95 0.0 96-97 0.0023462612327256518 98-99 0.0017596959245442388 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 85242.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.82468736069074 #Duplication Level Percentage of deduplicated Percentage of total 1 83.09495723975229 26.44471035405082 2 8.692126216455323 5.532483986767087 3 2.912120318490121 2.780319560779897 4 1.452373930993807 1.8488538513878137 5 0.7925390740194632 1.261115412590038 6 0.4976408139191979 0.9502357992538889 7 0.3907401946328517 0.8704629173412168 8 0.2727808905927455 0.6944933248867929 9 0.20642878207018578 0.5912578306468642 >10 1.4191978767325273 8.689378475399451 >50 0.13639044529637276 3.0477933413106215 >100 0.09215570628133295 5.830459163323244 >500 0.011058684753759953 2.517538302714624 >1k 0.014744913005013271 10.605100771919945 >5k 0.014744913005013271 28.335796907627696 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6790 7.965556885103588 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6716 7.878745219492739 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5440 6.381830553013773 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5208 6.109664250017597 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4713 5.528964594917999 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1835 2.1526946810257854 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1403 1.6459022547570448 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1089 1.2775392412191173 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 864 1.0135848525374815 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 736 0.8634241336430398 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 546 0.6405293165341029 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 418 0.4903685976396612 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 348 0.4082494544942634 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 344 0.4035569320288121 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 344 0.4035569320288121 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 322 0.3777480584688299 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 284 0.33316909504704256 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 247 0.28976326224161797 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 241 0.282724478543441 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 224 0.26278125806527297 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 208 0.2440111682034678 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 170 0.19943220478168042 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 164 0.19239342108350344 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 163 0.19122029046714062 No Hit GTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 157 0.18418150676896367 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 147 0.1724502006053354 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 135 0.15837263320898148 No Hit TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 128 0.1501607188944417 No Hit GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 127 0.1489875882780789 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 125 0.14664132704535324 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 121 0.14194880457990194 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 118 0.13842941273081344 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 114 0.13373689026536215 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 109 0.127871237183548 No Hit GAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 107 0.12552497595082235 No Hit GTACATGGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 105 0.12317871471809672 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 100 0.11731306163628259 No Hit GTACATGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 97 0.1137936697871941 No Hit ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 94 0.11027427793810562 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 94 0.11027427793810562 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 86 0.10088923300720302 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0029328265409070647 0.0 18-19 0.0 0.0 0.0 0.0046925224654513036 0.0 20-21 0.0 0.0 0.0 0.00821191431453978 0.0 22-23 0.0 0.0 0.0 0.010558175547265434 0.0 24-25 0.0 0.0 0.0 0.025222308251800756 0.0 26-27 0.0 0.0 0.0 0.035193918490884775 0.0 28-29 0.0 0.0 0.0 0.051617747119964336 0.0 30-31 0.0 0.0 0.0 0.07801318598812793 0.0 32-33 0.0 0.0 0.0 0.1003026676990216 0.0 34-35 0.0 0.0 0.0 0.12435184533445953 0.0 36-37 0.0 0.0 0.0 0.15192041481898594 0.0 38-39 0.0 0.0 0.0 0.1730367659135168 0.0 40-41 0.0 0.0 0.0 0.19356655169986625 0.0 42-43 0.0 0.0 0.0 0.25163651720982616 0.0 44-45 0.0 0.0 0.0 0.3173318317261444 0.0 46-47 0.0 0.0 0.0 0.3613242298397503 0.0 48-49 0.0 0.0 0.0 0.4158748035006218 0.0 50-51 0.0 0.0 0.0 0.4751178996269445 0.0 52-53 0.0 0.0 0.0 0.5589967386968865 0.0 54-55 0.0 0.0 0.0 0.6018160061941297 0.0 56-57 0.0 0.0 0.0 0.6851082799558903 0.0 58-59 0.0 0.0 0.0 0.8106332559067126 0.0 60-61 0.0 0.0 0.0 0.9091762276811901 0.0 62-63 0.0 0.0 0.0 1.0288355505501983 0.0 64-65 0.0 0.0 0.0 1.1355904366392156 0.0 66-67 0.0 0.0 0.0 1.255836324816405 0.0 68-69 0.0 0.0 0.0 1.4013045212453954 0.0 70-71 0.0 0.0 0.0 1.5121653644916826 0.0 72-73 0.0 0.0 0.0 1.6793364773233852 0.0 74-75 0.0 0.0 0.0 1.8177658900541986 0.0 76-77 0.0 0.0 0.0 1.9051641209732293 0.0 78-79 0.0 0.0 0.0 1.9831773069613572 0.0 80-81 0.0 0.0 0.0 2.1257126768494405 0.0 82-83 0.0 0.0 0.0 2.238333216020272 0.0 84-85 0.0 0.0 0.0 2.4260341146383237 0.0 86-87 0.0 0.0 0.0 2.710518289106309 0.0 88-89 0.0 0.0 0.0 2.978578634945215 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCAGAAA 30 1.2915011E-4 63.361523 8 CGCAGAA 25 0.0049965573 57.02537 7 AATGGGG 35 2.7752275E-4 54.278 3 GGGAATG 45 9.553927E-4 42.241013 7 GGTATCA 2065 0.0 41.85846 1 GATTTAA 25 0.0016130806 37.994602 22-23 GTATCAA 3205 0.0 33.637966 1 CAGAAAA 60 0.0039220667 31.680761 9 GGGAAGT 80 4.4475627E-4 29.700714 7 TGGGATG 170 3.5233825E-9 27.95361 6 AGTAATG 70 0.008330667 27.154936 7 TATCAAC 4060 0.0 26.671087 2 ATCAACG 4155 0.0 26.06128 3 TCAACGC 4180 0.0 25.90541 4 CAACGCA 4230 0.0 25.614233 5 GAGTACT 2935 0.0 25.486156 12-13 ATGGGTT 95 0.0012140959 25.011126 5 AACGCAG 4345 0.0 24.717556 6 TGGGGAA 155 1.3798381E-6 24.52704 6 ATGGGAT 375 0.0 24.077377 5 >>END_MODULE