##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936876_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 88575 Sequences flagged as poor quality 0 Sequence length 101 %GC 19 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.828856900931413 31.0 31.0 34.0 30.0 34.0 2 31.805407846457804 31.0 31.0 34.0 30.0 34.0 3 31.756296923511147 31.0 31.0 34.0 30.0 34.0 4 35.680033869602035 37.0 35.0 37.0 33.0 37.0 5 35.586316680779 37.0 35.0 37.0 33.0 37.0 6 35.82329099633079 37.0 35.0 37.0 35.0 37.0 7 35.73672029353655 37.0 35.0 37.0 33.0 37.0 8 35.761151566469096 37.0 35.0 37.0 35.0 37.0 9 37.243883714366355 39.0 37.0 39.0 34.0 39.0 10-11 37.22455546147333 39.0 37.0 39.0 33.5 39.0 12-13 36.95968952864804 39.0 37.0 39.0 32.5 39.0 14-15 37.56907705334463 39.5 36.5 41.0 32.0 41.0 16-17 37.57316963025684 39.0 36.5 40.5 32.5 41.0 18-19 37.673468811741465 39.0 35.5 41.0 33.0 41.0 20-21 37.84105560259667 39.5 35.0 41.0 33.0 41.0 22-23 37.6012305955405 39.5 35.0 41.0 33.0 41.0 24-25 37.36822466836015 39.0 35.0 41.0 33.0 41.0 26-27 36.90573525261078 38.0 35.0 41.0 33.0 41.0 28-29 36.45191081004798 37.5 35.0 40.5 33.0 41.0 30-31 35.35802427321479 35.5 35.0 39.0 31.0 41.0 32-33 34.34077335591307 35.0 33.5 37.5 30.0 41.0 34-35 33.79398814563929 35.0 33.0 36.5 28.0 40.0 36-37 33.265198983911944 35.0 33.0 35.0 24.5 40.0 38-39 32.79477843635337 35.0 33.0 35.0 22.5 40.0 40-41 32.12359017781541 35.0 33.0 35.0 20.5 40.0 42-43 31.50281117696867 35.0 33.0 35.0 19.0 40.0 44-45 30.81243014394581 35.0 32.0 35.0 13.5 40.0 46-47 29.773813152695453 35.0 30.0 35.0 2.0 39.5 48-49 28.9386677956534 35.0 26.0 35.0 2.0 39.0 50-51 28.595642111205194 35.0 27.0 35.0 2.0 39.0 52-53 28.3305955405024 35.0 25.5 35.0 2.0 39.0 54-55 27.884216765453004 35.0 23.0 35.0 2.0 38.0 56-57 27.62489415749365 34.5 23.0 35.0 2.0 38.0 58-59 27.302178944397404 33.5 23.0 35.0 2.0 38.0 60-61 26.84636748518205 33.0 22.0 35.0 2.0 38.0 62-63 26.35237933954276 33.0 21.0 35.0 2.0 37.0 64-65 26.092650296359018 33.0 20.0 35.0 2.0 36.0 66-67 25.945825571549534 33.0 20.0 35.0 2.0 36.0 68-69 25.53987581145922 33.0 16.5 35.0 2.0 35.5 70-71 25.13499294383291 33.0 7.5 35.0 2.0 35.0 72-73 24.700344340953993 33.0 2.0 35.0 2.0 35.0 74-75 24.21577758961332 33.0 2.0 35.0 2.0 35.0 76-77 22.727219870166525 30.0 2.0 33.5 2.0 35.0 78-79 23.23680496754163 32.5 2.0 35.0 2.0 35.0 80-81 22.912407564211122 33.0 2.0 35.0 2.0 35.0 82-83 22.4214733276884 31.5 2.0 35.0 2.0 35.0 84-85 21.75778154106689 31.0 2.0 35.0 2.0 35.0 86-87 21.067677109793962 30.5 2.0 35.0 2.0 35.0 88-89 20.336421112051934 29.0 2.0 35.0 2.0 35.0 90-91 19.48739486311036 28.0 2.0 34.5 2.0 35.0 92-93 18.647496471916455 26.0 2.0 34.0 2.0 35.0 94-95 17.76116285633644 22.0 2.0 33.5 2.0 35.0 96-97 16.747440022579735 7.5 2.0 33.0 2.0 35.0 98-99 15.743398250070562 2.0 2.0 33.0 2.0 35.0 100-101 13.269793959920971 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.03180718910029512 1101 2 0.22776249642455682 1101 3 0.1379444283902238 1101 4 -0.0997819893450469 1101 5 -0.16698808237076435 1101 6 -0.06143403913055323 1101 7 -0.19345132063838122 1101 8 0.14024973875522306 1101 9 0.08809705088186348 1101 10-11 0.08784589686450062 1101 12-13 -0.042878502821260156 1101 14-15 0.6029162954973728 1101 16-17 0.2232276313615884 1101 18-19 -0.005309796570450942 1101 20-21 0.31445419220630555 1101 22-23 0.6728747364176542 1101 24-25 0.809371945322475 1101 26-27 0.837644971757868 1101 28-29 1.031716475504794 1101 30-31 1.216982054450007 1101 32-33 1.2163814886669044 1101 34-35 0.49635841378488266 1101 36-37 0.6505218844987652 1101 38-39 0.9770961886075256 1101 40-41 0.9981553896587059 1101 42-43 0.41047420970968673 1101 44-45 0.5822485656194374 1101 46-47 0.6452465441698507 1101 48-49 1.044942428633913 1101 50-51 1.211931580349738 1101 52-53 1.462010926971903 1101 54-55 1.1281662646802424 1101 56-57 1.5123964807457142 1101 58-59 2.2890511651316636 1101 60-61 1.7909909998547668 1101 62-63 2.2146727545356093 1101 64-65 2.015177655803406 1101 66-67 2.7146164678116307 1101 68-69 2.704213972114143 1101 70-71 2.3687157067703275 1101 72-73 2.460488358245552 1101 74-75 2.0219154730800497 1101 76-77 1.1918945167550987 1101 78-79 1.3160479512780086 1101 80-81 1.9142551988698422 1101 82-83 2.728642799891862 1101 84-85 2.5972606403981757 1101 86-87 2.8945333750201527 1101 88-89 3.557560479074457 1101 90-91 3.2621724748785255 1101 92-93 3.209999185040097 1101 94-95 2.855689402415557 1101 96-97 2.1326019493566406 1101 98-99 2.1696056784322 1101 100-101 1.902419236472527 1102 1 -0.3553447405528587 1102 2 0.11540294586275834 1102 3 -0.1694566463533036 1102 4 -0.014046767068542465 1102 5 -0.3129333681548374 1102 6 0.10881510595982036 1102 7 0.2065242533723648 1102 8 -0.1562817707709101 1102 9 -0.14846865502922668 1102 10-11 -0.2273714944398435 1102 12-13 -0.21166208855647284 1102 14-15 -0.6239400796858234 1102 16-17 -1.2614450603824352 1102 18-19 -0.2586073051195541 1102 20-21 -0.1595931942128459 1102 22-23 0.2302147461880537 1102 24-25 0.3902219697484668 1102 26-27 0.3558423337510277 1102 28-29 0.10835301678471865 1102 30-31 -0.14873606963401187 1102 32-33 -0.44947097835801486 1102 34-35 -0.3095160366303631 1102 36-37 -0.6123628248319548 1102 38-39 -0.11364636146575435 1102 40-41 -0.36682995474774316 1102 42-43 -0.014049483499888993 1102 44-45 0.041518228540788016 1102 46-47 -0.6633147650631734 1102 48-49 -0.9157927936425914 1102 50-51 -0.10621692966492091 1102 52-53 0.25512279800267734 1102 54-55 0.37205976736710156 1102 56-57 -0.187469176313396 1102 58-59 -0.11544321689080661 1102 60-61 -0.3583128594515337 1102 62-63 0.09162683367581437 1102 64-65 0.27800130016100155 1102 66-67 -0.18022964845607703 1102 68-69 -0.3249140200695386 1102 70-71 0.05862777320901813 1102 72-73 -0.3122278117593389 1102 74-75 -0.45610113659263973 1102 76-77 -0.3793683068892584 1102 78-79 -0.8753296745158359 1102 80-81 -0.9325327835434472 1102 82-83 0.45665940956455486 1102 84-85 0.3791976213459023 1102 86-87 -0.07414029376342768 1102 88-89 -0.39193878814586824 1102 90-91 -0.6297425226788746 1102 92-93 -0.11120257363112529 1102 94-95 -0.4139735189327567 1102 96-97 -0.4917874009120453 1102 98-99 -0.07453208414718482 1102 100-101 0.05813491795762715 1103 1 -0.02220446486653671 1103 2 -0.029969462338328867 1103 3 0.20596325402895133 1103 4 0.26395624463074796 1103 5 -0.19466625359443412 1103 6 -0.2530521476020908 1103 7 0.06775441669913818 1103 8 0.05418967600309088 1103 9 -0.18588322702042603 1103 10-11 -0.2273714944398435 1103 12-13 0.20319948911060948 1103 14-15 -0.026117769944129066 1103 16-17 0.12590580258524398 1103 18-19 0.10024824914895447 1103 20-21 0.09779126078542077 1103 22-23 0.21405582113303012 1103 24-25 0.7853916674201926 1103 26-27 0.589886119225369 1103 28-29 0.802839290386018 1103 30-31 0.30029671155445214 1103 32-33 0.8909668763854128 1103 34-35 0.8988124702617952 1103 36-37 0.7844864743687836 1103 38-39 0.5213588510907172 1103 40-41 0.7452979266376403 1103 42-43 0.38268398111264545 1103 44-45 -0.22316380551458082 1103 46-47 1.156620367567431 1103 48-49 0.8422866688849204 1103 50-51 1.158928442743285 1103 52-53 1.1529566912031868 1103 54-55 1.5558892588532593 1103 56-57 1.3592700800285478 1103 58-59 0.7942058043069231 1103 60-61 0.8131223310963662 1103 62-63 1.4767189221734434 1103 64-65 1.4981449350503837 1103 66-67 1.2548450648533134 1103 68-69 1.5889629635977833 1103 70-71 1.5868914127317808 1103 72-73 2.1421333604866994 1103 74-75 1.8748961992118325 1103 76-77 2.5225197968985356 1103 78-79 2.3836185474232323 1103 80-81 2.877556409089472 1103 82-83 3.625662073760079 1103 84-85 2.867150824172267 1103 86-87 2.6198247241909307 1103 88-89 3.581708843036804 1103 90-91 3.4422486738924185 1103 92-93 3.127300843489227 1103 94-95 3.8247615673640105 1103 96-97 3.451685320042433 1103 98-99 1.7972390267065155 1103 100-101 2.803472575782253 1104 1 0.13021766128355594 1104 2 0.1955044319084287 1104 3 0.36503652750196025 1104 4 0.05566448096818277 1104 5 0.27024894427719914 1104 6 0.2749380973818347 1104 7 0.25243460736535894 1104 8 0.28143720953595874 1104 9 0.1707291219431042 1104 10-11 -0.09296289228930021 1104 12-13 0.1794216374311972 1104 14-15 0.0910532753945219 1104 16-17 0.4943239380469535 1104 18-19 0.7457420968376809 1104 20-21 1.039150311888399 1104 22-23 0.9825404680679668 1104 24-25 1.018348102405227 1104 26-27 1.0128436627349586 1104 28-29 1.0592410196843218 1104 30-31 0.6687702732438439 1104 32-33 1.1259186555673182 1104 34-35 1.468483238469311 1104 36-37 1.110725250557671 1104 38-39 0.8680499052975676 1104 40-41 0.7346560909209785 1104 42-43 1.402526570626005 1104 44-45 1.3819797484151373 1104 46-47 1.4336406535667656 1104 48-49 1.363958323912076 1104 50-51 1.5643158720748396 1104 52-53 0.5889393982201447 1104 54-55 0.5431966527120373 1104 56-57 0.8664754896283711 1104 58-59 1.3159328855617716 1104 60-61 1.8977581807804356 1104 62-63 2.2467555034837154 1104 64-65 1.5083987871732063 1104 66-67 1.078922218162262 1104 68-69 1.2565164499075046 1104 70-71 1.4765932196268032 1104 72-73 1.9704229071001542 1104 74-75 2.681569517025821 1104 76-77 2.8974228010000758 1104 78-79 2.9451970863878714 1104 80-81 2.7721911502489895 1104 82-83 3.2921070355159827 1104 84-85 3.5342054744327704 1104 86-87 2.6694866242020154 1104 88-89 2.742167772202084 1104 90-91 3.206188370156692 1104 92-93 3.9809201761574435 1104 94-95 3.936334563705877 1104 96-97 3.9679806309835683 1104 98-99 3.7188109145238304 1104 100-101 4.3312411158554145 1105 1 0.2371725915054732 1105 2 -0.12330133336267579 1105 3 -0.11483077975038469 1105 4 0.2609950689343279 1105 5 -0.5252211998545775 1105 6 -0.15116576901029788 1105 7 0.13564211136902316 1105 8 -0.2293154786360816 1105 9 -0.15265225531610582 1105 10-11 0.04922425024100363 1105 12-13 0.41294943427403297 1105 14-15 0.4358256386981125 1105 16-17 0.45114159990922786 1105 18-19 0.2308141627264959 1105 20-21 0.33353374817300363 1105 22-23 0.6207239501086832 1105 24-25 0.786707745507492 1105 26-27 -0.10823846950022897 1105 28-29 -0.2702030434591194 1105 30-31 -0.08054338495794156 1105 32-33 0.32461460615071047 1105 34-35 -0.702415869286348 1105 36-37 -0.6090757103208446 1105 38-39 -0.38229326560153964 1105 40-41 -0.11898152060956235 1105 42-43 -0.052060480392071895 1105 44-45 -0.13317696629545495 1105 46-47 -0.6670800053213632 1105 48-49 -0.7706218636880138 1105 50-51 -0.5695207785771821 1105 52-53 -0.18044975757944215 1105 54-55 0.3802476707857032 1105 56-57 -0.34017058678434964 1105 58-59 -0.8386084093364197 1105 60-61 -0.23178883363274494 1105 62-63 -0.014397550737282216 1105 64-65 -0.6418528714861438 1105 66-67 -1.192182792132865 1105 68-69 -1.6071875079968656 1105 70-71 -1.5880372997051566 1105 72-73 -0.833683704659169 1105 74-75 -1.4174924825166215 1105 76-77 -1.4173793037814413 1105 78-79 -1.6996782281854514 1105 80-81 -1.818977918613971 1105 82-83 -1.2681194572774288 1105 84-85 -1.2402740497035794 1105 86-87 -1.162474025534884 1105 88-89 -0.8909825366517232 1105 90-91 -0.859242881273186 1105 92-93 -0.823072045302176 1105 94-95 -0.7772893209887002 1105 96-97 -0.48569129763688323 1105 98-99 -0.3660094927056363 1105 100-101 -0.13735874687622562 1106 1 0.06633404332024284 1106 2 0.3924683787774974 1106 3 0.022215528134470475 1106 4 0.26262210272656716 1106 5 0.2960553958901002 1106 6 0.5388217153451507 1106 7 0.45041057194474377 1106 8 0.3648021684227416 1106 9 0.73758554193045 1106 10-11 0.5785108585013319 1106 12-13 0.41926983477464574 1106 14-15 0.47024366122627015 1106 16-17 0.4528312119242415 1106 18-19 0.17192805509194642 1106 20-21 -0.02998314794714929 1106 22-23 0.3423760151900339 1106 24-25 -0.05584544598186625 1106 26-27 -0.5277002967843316 1106 28-29 0.050322613612209466 1106 30-31 0.06269816698198127 1106 32-33 -0.0025443425351454607 1106 34-35 0.06279063884881708 1106 36-37 0.19649375150644488 1106 38-39 -0.387737570983262 1106 40-41 0.2691911952853019 1106 42-43 0.40075553963296073 1106 44-45 -0.6486337873217387 1106 46-47 -0.40468951721122437 1106 48-49 -0.8689322516729945 1106 50-51 -1.2927998774507756 1106 52-53 -0.8807000704705565 1106 54-55 -0.7857723542455872 1106 56-57 -0.9288439284614434 1106 58-59 -2.0815370701624545 1106 60-61 -1.832602350528866 1106 62-63 -1.9016315932904782 1106 64-65 -1.950551244452349 1106 66-67 -2.1637722789914378 1106 68-69 -2.2728320636914816 1106 70-71 -2.281028538753965 1106 72-73 -1.7214809512173659 1106 74-75 -1.5890819693751936 1106 76-77 -1.6527985778740586 1106 78-79 -2.0789648364457776 1106 80-81 -2.7624072052222317 1106 82-83 -2.233889169417605 1106 84-85 -2.0551050885020814 1106 86-87 -2.2877556275373863 1106 88-89 -1.621020083585389 1106 90-91 -2.2443492642519054 1106 92-93 -3.0119956998703863 1106 94-95 -2.709016479937384 1106 96-97 -2.653651247574306 1106 98-99 -3.3837191297519436 1106 100-101 -2.864204804448189 1107 1 -0.15719536844446225 1107 2 -0.20753162122250046 1107 3 -0.2248432953949404 1107 4 -0.10208377962637627 1107 5 0.10782010177245382 1107 6 -0.3082371081842652 1107 7 -0.43194236923172724 1107 8 0.047155109599209766 1107 9 -0.3565321051283732 1107 10-11 -0.30972443561631025 1107 12-13 -0.27484781228417177 1107 14-15 -0.9062269270090226 1107 16-17 0.11165474133600384 1107 18-19 -0.18101312137864056 1107 20-21 -0.02998314794714929 1107 22-23 -0.21644751422172703 1107 24-25 -0.17937485774657347 1107 26-27 -0.5218179438431605 1107 28-29 -0.11438326874072402 1107 30-31 0.05681581404080305 1107 32-33 -0.02607375429985126 1107 34-35 -0.1254446552688293 1107 36-37 -0.8035062484935551 1107 38-39 -0.9406787474538518 1107 40-41 -0.7190440988323417 1107 42-43 -1.6580679897788038 1107 44-45 -1.6486337873217387 1107 46-47 -1.1576306936818135 1107 48-49 -1.0571675457906444 1107 50-51 -1.481035171568422 1107 52-53 -0.9689353645882015 1107 54-55 -0.45636058953970604 1107 56-57 -1.0582556931673288 1107 58-59 -0.4521253054565726 1107 60-61 0.09092706123584193 1107 62-63 0.06307428906246315 1107 64-65 0.19062522613588584 1107 66-67 0.018580662185030405 1107 68-69 -0.10812618133854457 1107 70-71 0.9719126377166205 1107 72-73 0.7255778723120478 1107 74-75 0.36385920709539477 1107 76-77 -0.9704456366975869 1107 78-79 -0.26131777762224573 1107 80-81 0.055239853601296574 1107 82-83 0.19552259528827776 1107 84-85 -0.45510508850208 1107 86-87 -0.2465791569491529 1107 88-89 -0.9386671424089208 1107 90-91 -2.0443492642519097 1107 92-93 -2.27081922928215 1107 94-95 -1.6913694211138548 1107 96-97 -2.7536512475743056 1107 98-99 -1.971954423869592 1107 100-101 -1.140675392683482 1108 1 -0.10949715872579446 1108 2 -4.983731406618119E-4 1108 3 -0.18814252813151455 1108 4 -0.06717329369286773 1108 5 -0.04742286497945969 1108 6 0.27552248260857226 1108 7 -0.03040784237750671 1108 8 0.020684521363918407 1108 9 -0.07494642737901103 1108 10-11 -0.2654149727007109 1108 12-13 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-0.7972634623167245 2316 56-57 -1.5989533675859313 2316 58-59 -1.4318790674264754 2316 60-61 -1.6633821042908323 2316 62-63 -1.3481432211974536 2316 64-65 -3.0571175919215676 2316 66-67 -3.742158051905257 2316 68-69 -3.2165393140334793 2316 70-71 -3.203326760368192 2316 72-73 -3.098156737811074 2316 74-75 -2.955839835312265 2316 76-77 -3.403277374043686 2316 78-79 -3.364942948620879 2316 80-81 -3.507003648450688 2316 82-83 -3.5668577056693103 2316 84-85 -3.44710235252397 2316 86-87 -3.5348144510668007 2316 88-89 -4.372798469358305 2316 90-91 -3.845512054949584 2316 92-93 -4.030395152674764 2316 94-95 -4.853612923165839 2316 96-97 -4.7004364732925 2316 98-99 -4.5798887603948994 2316 100-101 -3.5717971437094764 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 4.0 5 27.0 6 101.0 7 111.0 8 166.0 9 293.0 10 445.0 11 604.0 12 762.0 13 984.0 14 1284.0 15 1806.0 16 2326.0 17 2894.0 18 2635.0 19 1717.0 20 1582.0 21 1683.0 22 1736.0 23 1929.0 24 2098.0 25 2350.0 26 2561.0 27 2721.0 28 3197.0 29 3491.0 30 3920.0 31 4355.0 32 4739.0 33 5874.0 34 8501.0 35 12169.0 36 3689.0 37 3427.0 38 2244.0 39 146.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.015243730310175 20.32497374690891 23.114011811068078 6.545770711712831 2 14.603608282340192 29.11915460518888 42.944882245354165 13.332354867116761 3 17.367595091054834 38.16061327944182 37.55772075011573 6.914070879387624 4 8.048546429579453 24.712390629410105 38.40474174428451 28.834321196725938 5 5.647446681193618 44.14989104785991 32.658545121992525 17.544117148953948 6 15.173581710414902 41.53655094552639 22.705052215636467 20.58481512842224 7 14.70183123715763 47.66974507191727 23.19755232912593 14.430871361799175 8 26.454417160598364 29.825571549534292 23.19954840530624 20.520462884561105 9 29.282528930285068 16.938187976291278 24.69093988145639 29.08834321196726 10-11 21.556308213378493 33.75331639853232 24.61191081004798 20.078464578041206 12-13 28.238276156013075 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4.926500474190489 38-39 6.276568718773816 34.788155926270925 53.97239393682784 4.962881418127417 40-41 5.58058142816822 35.228337567033584 53.832345469940726 5.358735534857465 42-43 5.209144792548687 35.95992097092859 53.18035563082134 5.650578605701384 44-45 5.403361012481018 36.15600426759394 52.142546669752576 6.298088050172454 46-47 5.448489980242732 36.09991532599492 50.804967541631385 7.646627152130963 48-49 5.799604854642958 36.11120519333898 50.15128422241039 7.937905729607678 50-51 6.12655026616313 36.714141362822964 49.254573884965595 7.90473448604831 52-53 5.490826982782952 36.75698560541913 49.2695455828394 8.48264182895851 54-55 5.14422805532035 36.60005644933672 48.75585661868473 9.499858876658198 56-57 5.6415785581629025 36.69227599365506 47.871565744091136 9.794579704090907 58-59 5.593031854540528 35.8263382802048 48.300018628386276 10.280611236868399 60-61 5.737704918032787 35.537754595131645 48.51533215914736 10.20920832768821 62-63 6.498447643240192 35.39881456392887 48.572396274343774 9.530341518487157 64-65 6.345469940728196 35.43945808636749 49.0956816257409 9.11939034716342 66-67 5.726785210273779 35.38639570985041 49.297205757832344 9.589613322043466 68-69 5.58848433530906 35.55574372001129 49.27406152977702 9.581710414902625 70-71 5.664690939881456 35.28083545018346 48.728196443691786 10.326277166243298 72-73 5.68275472763195 34.659328252893026 48.56957380750776 11.08834321196726 74-75 5.919975613335893 34.411163672902575 48.751876982827724 10.916983730933806 76-77 5.907423087778719 34.091447925486875 48.87101326559413 11.130115721140276 78-79 5.697431555179227 33.8842788597234 48.82020886254587 11.59808072255151 80-81 5.490888974191072 34.73028202407027 47.86449748233116 11.9143315194075 82-83 5.529777025119955 34.41716059836297 47.21704769968953 12.836014676827547 84-85 5.838056314494096 33.57700905457583 46.903718924289294 13.681215706640776 86-87 6.258537962178945 33.19785492520463 46.68755292125317 13.856054191363251 88-89 6.754201265601273 32.28920287441645 46.73749216761032 14.21910369237196 90-91 6.503564795736922 32.03913090110585 46.79676430575391 14.660539997403315 92-93 6.448772226926333 32.18233135760655 46.12644651425345 15.24244990121366 94-95 6.662715213096246 31.880327406152976 45.5388089189952 15.918148461755575 96-97 6.885690093141406 31.18882303132938 45.00479819362123 16.92068868190799 98-99 7.131244707874683 30.75303415184872 44.867626305390914 17.24809483488569 100-101 7.389866100661593 30.569918937837286 45.00417729807844 17.036037663422672 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5707.0 1 3904.5 2 2116.5 3 2031.5 4 1536.0 5 1383.5 6 2547.5 7 4996.5 8 6764.5 9 6517.5 10 4632.5 11 3001.0 12 2657.0 13 2368.0 14 1924.5 15 1425.0 16 1082.0 17 903.0 18 749.0 19 665.0 20 659.0 21 635.5 22 603.0 23 587.5 24 612.5 25 667.0 26 749.0 27 823.5 28 888.0 29 992.0 30 1095.0 31 1139.0 32 1236.0 33 1350.5 34 1371.0 35 1419.0 36 1469.0 37 1407.0 38 1355.5 39 1293.0 40 1142.5 41 1018.0 42 916.5 43 832.0 44 756.0 45 686.0 46 693.5 47 792.5 48 1789.0 49 1798.5 50 869.0 51 677.5 52 375.5 53 248.5 54 199.0 55 174.5 56 178.5 57 163.5 58 137.0 59 121.0 60 112.5 61 96.5 62 85.5 63 57.5 64 31.5 65 21.5 66 24.0 67 22.0 68 15.0 69 15.0 70 19.0 71 15.5 72 10.0 73 13.5 74 13.5 75 9.5 76 7.5 77 8.5 78 7.5 79 4.0 80 2.5 81 2.0 82 1.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01580581428168219 2 0.0011289867344058708 3 0.0022579734688117415 4 0.0 5 0.004515946937623483 6 0.0 7 0.0011289867344058708 8 0.0 9 0.0 10-11 0.0 12-13 0.006209427039232289 14-15 0.002822466836014677 16-17 0.0 18-19 0.0 20-21 0.0022579734688117415 22-23 0.002822466836014677 24-25 5.644933672029354E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.003386960203217612 38-39 0.00846740050804403 40-41 0.0 42-43 0.0 44-45 5.644933672029354E-4 46-47 0.0 48-49 0.0 50-51 0.001693480101608806 52-53 0.0 54-55 0.0 56-57 5.644933672029354E-4 58-59 5.644933672029354E-4 60-61 0.003386960203217612 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0022579734688117415 76-77 0.0 78-79 0.0 80-81 0.0011289867344058708 82-83 0.0 84-85 0.0011289867344058708 86-87 0.0 88-89 5.644933672029354E-4 90-91 5.644933672029354E-4 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0011289867344058708 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 88575.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.26418289585097 #Duplication Level Percentage of deduplicated Percentage of total 1 82.9379242774162 26.759243578887947 2 8.985933235355867 5.798475867908552 3 2.9498215410455595 2.855207451312447 4 1.3226957799706067 1.7070279424216768 5 0.699839037021485 1.1289867344058706 6 0.5073833018405767 0.9822184589331074 7 0.3884106655469242 0.8772226926333615 8 0.2484428581426272 0.6412644651425345 9 0.2484428581426272 0.7214225232853514 >10 1.4416684162642592 8.876093705898956 >50 0.139967807404297 3.4016370307648884 >100 0.09097907481279305 5.579452441433813 >500 0.010497585555322275 2.4848998024273214 >1k 0.013996780740429702 10.375388089189952 >5k 0.013996780740429702 27.81145921535422 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6955 7.852102737792831 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6854 7.738075077617839 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5430 6.130397967823878 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5395 6.090883432119672 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4807 5.427039232289021 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1846 2.0841095117132373 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1407 1.58848433530906 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1130 1.2757550098786339 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 871 0.9833474456675133 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 761 0.8591589048828676 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 569 0.6423934518769404 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 387 0.436917866215072 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 375 0.42337002540220153 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 365 0.4120801580581428 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 354 0.39966130397967825 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 330 0.37256562235393736 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 268 0.30256844482077333 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 250 0.28224668360146765 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 241 0.27208580299181484 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 187 0.2111205193338978 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 180 0.20321761219305673 No Hit GTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 172 0.19418571831780976 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 157 0.1772509173017217 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 157 0.1772509173017217 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 152 0.17160598362969234 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 142 0.16031611628563364 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 129 0.14563928873835733 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 125 0.14112334180073383 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 120 0.1354784081287045 No Hit GTACATGGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 113 0.12757550098786338 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 109 0.12305955405023991 No Hit GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 109 0.12305955405023991 No Hit GAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 108 0.12193056731583404 No Hit GTACATGGGGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAAA 105 0.11854360711261643 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 105 0.11854360711261643 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 101 0.11402766017499294 No Hit TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 101 0.11402766017499294 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 100 0.11289867344058707 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 100 0.11289867344058707 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 100 0.11289867344058707 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 99 0.11176968670618119 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 98 0.11064069997177534 No Hit GTACATGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 96 0.10838272650296359 No Hit GAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 94 0.10612475303415185 No Hit GTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 93 0.10499576629974597 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 92 0.10386677956534011 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 89 0.10047981936212248 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0022579734688117415 0.0 20-21 0.0 0.0 0.0 0.003951453570420547 0.0 22-23 0.0 0.0 0.0 0.009031893875246966 0.0 24-25 0.0 0.0 0.0 0.017499294383290995 0.0 26-27 0.0 0.0 0.0 0.024837708156929157 0.0 28-29 0.0 0.0 0.0 0.04290149590742309 0.0 30-31 0.0 0.0 0.0 0.08241603161162855 0.0 32-33 0.0 0.0 0.0 0.10668924640135478 0.0 34-35 0.0 0.0 0.0 0.1275755009878634 0.0 36-37 0.0 0.0 0.0 0.15184871577758963 0.0 38-39 0.0 0.0 0.0 0.1896697713801863 0.0 40-41 0.0 0.0 0.0 0.21168501270110074 0.0 42-43 0.0 0.0 0.0 0.26700536268698843 0.0 44-45 0.0 0.0 0.0 0.3330510866497319 0.0 46-47 0.0 0.0 0.0 0.37708156929156084 0.0 48-49 0.0 0.0 0.0 0.4470787468247248 0.0 50-51 0.0 0.0 0.0 0.5176404177250917 0.0 52-53 0.0 0.0 0.0 0.6090883432119673 0.0 54-55 0.0 0.0 0.0 0.6621507197290432 0.0 56-57 0.0 0.0 0.0 0.7552921253175275 0.0 58-59 0.0 0.0 0.0 0.8687552921253175 0.0 60-61 0.0 0.0 0.0 0.9556872706745696 0.0 62-63 0.0 0.0 0.0 1.0872142252328536 0.0 64-65 0.0 0.0 0.0 1.1905165114309906 0.0 66-67 0.0 0.0 0.0 1.3220434659892746 0.0 68-69 0.0 0.0 0.0 1.4840530623765171 0.0 70-71 0.0 0.0 0.0 1.607112616426757 0.0 72-73 0.0 0.0 0.0 1.7696867061812025 0.0 74-75 0.0 0.0 0.0 1.9328252893028508 0.0 76-77 0.0 0.0 0.0 2.0112898673440585 0.0 78-79 0.0 0.0 0.0 2.0824160316116282 0.0 80-81 0.0 0.0 0.0 2.225232853513971 0.0 82-83 0.0 0.0 0.0 2.3449054473609934 0.0 84-85 0.0 0.0 0.0 2.530059271803556 0.0 86-87 0.0 0.0 0.0 2.7840812870448772 0.0 88-89 0.0 0.0 0.0 3.066327970646345 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGTGGA 20 0.0020637913 71.28432 9 GCAGAGG 40 7.466948E-6 59.4036 9 TGGGTGG 25 0.0049964823 57.027454 8 GGGTGAG 25 0.0049964823 57.027454 7 TGGGTGA 120 0.0 55.44336 6 ATGGGTG 170 0.0 50.289932 5 AGAGTAA 80 4.152753E-9 47.496048 5 GAATTGA 20 5.3779734E-4 47.496048 14-15 GGTATCA 1860 0.0 46.47463 1 AGTAATG 65 2.5537702E-6 43.867275 7 GGGTGAA 125 3.6379788E-12 41.820137 7 GAGTAAT 70 4.2510856E-6 40.7339 6 TGGGAGT 60 8.205521E-5 39.602398 6 GGTGAAA 135 7.2759576E-12 38.722347 8 GTATCAA 3130 0.0 38.391373 1 CATGGGT 260 0.0 38.36219 4 GTAATGG 75 6.8276004E-6 38.018307 8 CGCAGAA 50 0.0016039686 38.018303 9 ACCACAC 25 0.0016127861 37.996838 90-91 GAACGCA 65 1.3145726E-4 36.55606 9 >>END_MODULE