FastQCFastQC Report
Thu 26 May 2016
SRR936875_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936875_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254160
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59330.47306563755820624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44110.35170951074822987No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30370.24215411111819865No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20210.16114371372073738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76250.030.4222351
GGTATCA51850.026.7698081
GTACATG54950.025.3460961
TATCAAC90800.025.3389112
ATCAACG91100.025.1501033
CAACGCA91400.025.0655525
GTACCGT950.001222537425.0142866
TCAACGC91650.024.947314
AACGCAG93800.024.6249396
TACATGG55700.024.4937442
ACATGGG56000.023.342953
GGTATAC1703.1294367E-622.369293
GTGTAGC6050.021.999551
GAGTACT47500.021.8594312-13
ACGCAGA104950.021.782337
AGAGTAC78350.021.44460910-11
CGCAGAG105600.021.4249328
AGTACTT50300.021.11497512-13
GCAGAGT108800.020.534319
GTACTTT52450.019.70504414-15
Produced by FastQC (version 0.11.5)