##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936875_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1254160 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.613600338074885 33.0 31.0 34.0 30.0 34.0 2 31.714858550743127 33.0 31.0 34.0 30.0 34.0 3 31.722477993238503 33.0 31.0 34.0 30.0 34.0 4 35.29405897174204 37.0 35.0 37.0 33.0 37.0 5 35.323714677553106 37.0 35.0 37.0 33.0 37.0 6 35.378372775403456 37.0 35.0 37.0 33.0 37.0 7 35.314205524016074 37.0 35.0 37.0 33.0 37.0 8 35.27957597116795 37.0 35.0 37.0 33.0 37.0 9 36.87673103910187 39.0 37.0 39.0 33.0 39.0 10-11 36.74020659246029 39.0 37.0 39.0 32.0 39.0 12-13 36.627607322829626 39.0 37.0 39.0 32.0 39.0 14-15 37.74127304331186 40.0 37.5 41.0 32.0 41.0 16-17 37.67528784206162 40.0 37.0 41.0 32.0 41.0 18-19 37.63584749952159 40.0 37.0 41.0 32.0 41.0 20-21 37.582969477578615 40.0 37.0 41.0 32.0 41.0 22-23 37.434806165082605 40.0 37.0 41.0 32.0 41.0 24-25 37.25889160872616 40.0 37.0 41.0 31.0 41.0 26-27 37.21894534987561 39.5 36.5 41.0 31.0 41.0 28-29 37.28554889328315 40.0 37.0 41.0 31.0 41.0 30-31 37.14578243605282 39.5 37.0 41.0 31.0 41.0 32-33 36.961351023792815 39.0 36.0 41.0 30.5 41.0 34-35 36.76868900299802 39.0 36.0 41.0 30.0 41.0 36-37 36.61411063979077 39.0 35.5 41.0 30.0 41.0 38-39 36.45112067040888 39.0 35.0 41.0 30.0 41.0 40-41 36.24850218472922 39.0 35.0 41.0 30.0 41.0 42-43 36.04157563628245 39.0 35.0 40.5 29.0 41.0 44-45 35.78314848185239 39.0 35.0 40.0 28.5 41.0 46-47 35.42944241564075 38.5 35.0 40.0 26.5 41.0 48-49 35.08867887669835 38.0 34.5 40.0 25.5 41.0 50-51 34.44732370670409 37.5 33.5 39.5 24.0 40.5 52-53 34.742479428462076 38.0 34.5 40.0 25.5 40.5 54-55 35.04912690565797 39.0 35.0 40.0 26.0 41.0 56-57 34.930630063149835 38.5 34.5 40.0 25.5 41.0 58-59 34.68811196338585 38.0 34.0 40.0 24.5 41.0 60-61 34.40195190406328 38.0 34.0 40.0 23.5 41.0 62-63 34.09497312942527 38.0 34.0 40.0 23.0 41.0 64-65 33.785442846207815 37.0 33.5 40.0 22.5 41.0 66-67 33.41296485296932 36.5 33.0 39.5 22.0 41.0 68-69 32.97642605409198 36.0 32.5 39.0 20.0 41.0 70-71 32.620958250940866 35.5 32.0 39.0 20.0 40.5 72-73 32.238290568986415 35.0 32.0 38.5 18.5 40.0 74-75 31.80337795815526 35.0 31.5 37.5 18.0 39.5 76-77 31.358563899342986 35.0 31.0 37.0 14.5 39.0 78-79 30.92893889136952 35.0 31.0 36.0 11.0 39.0 80-81 30.491647796134465 35.0 30.5 36.0 7.5 37.5 82-83 30.07525554953116 34.0 30.0 35.5 6.5 37.0 84-85 29.718223751355488 34.0 30.0 35.0 2.0 37.0 86-87 29.392273713082858 34.0 29.5 35.0 2.0 36.0 88-89 29.00748748166103 34.0 29.0 35.0 2.0 36.0 90-91 28.662079001084393 34.0 29.0 35.0 2.0 36.0 92-93 28.291007128277094 34.0 29.0 35.0 2.0 35.0 94-95 27.881985552082668 34.0 28.0 35.0 2.0 35.0 96-97 27.43598185239523 34.0 27.0 35.0 2.0 35.0 98-99 26.821399980863685 34.0 25.5 35.0 2.0 35.0 100-101 24.67035306499968 31.0 20.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6328024136759929 1101 2 0.530584581294054 1101 3 0.5365846585970715 1101 4 0.43676509232357574 1101 5 0.4037505180159684 1101 6 0.43087340585797307 1101 7 0.5946968005484052 1101 8 0.3192600320986614 1101 9 0.573905035788357 1101 10-11 0.5176940454397396 1101 12-13 0.7594050152347549 1101 14-15 1.031150322641004 1101 16-17 0.998792627314252 1101 18-19 0.9161485679225834 1101 20-21 0.8956209544951079 1101 22-23 0.948310193589954 1101 24-25 1.0177488753214163 1101 26-27 1.2699983894556723 1101 28-29 1.1382768939192829 1101 30-31 0.8842813524970126 1101 32-33 0.824101753725138 1101 34-35 0.8768240919221313 1101 36-37 0.9601416444864341 1101 38-39 0.9291710381186178 1101 40-41 1.2113256667394054 1101 42-43 1.2132701446305916 1101 44-45 1.2163134449706021 1101 46-47 1.4471054251783926 1101 48-49 1.346498662998279 1101 50-51 1.2731142577367507 1101 52-53 1.2278065854614866 1101 54-55 1.4071374534816883 1101 56-57 1.2130991091043768 1101 58-59 1.0808832299083875 1101 60-61 1.4698688778838829 1101 62-63 1.55440290348119 1101 64-65 1.3714149044622488 1101 66-67 1.0931875932732211 1101 68-69 1.093111204011528 1101 70-71 1.2233577687564363 1101 72-73 1.2640250400017266 1101 74-75 1.1790440483393958 1101 76-77 1.2966816347667027 1101 78-79 1.3380111064077873 1101 80-81 1.365390369269928 1101 82-83 1.0964848737001276 1101 84-85 1.3561205420053994 1101 86-87 1.4299090459377375 1101 88-89 1.5349327301184346 1101 90-91 1.6023859852401365 1101 92-93 1.326586136174516 1101 94-95 1.3089605825615394 1101 96-97 1.3418981486964512 1101 98-99 1.560527263788348 1101 100-101 1.6331826339854203 1102 1 0.5121212107005242 1102 2 0.6289455165627196 1102 3 0.43972850837433697 1102 4 0.3515188063291603 1102 5 0.45454873614585267 1102 6 0.3814734015951302 1102 7 0.3945902293695127 1102 8 0.4996807751129211 1102 9 0.5824243358288541 1102 10-11 0.6427947552037878 1102 12-13 0.6099058997755407 1102 14-15 0.6458773938518618 1102 16-17 0.5376388877315819 1102 18-19 0.5986250689776327 1102 20-21 0.7430920204200433 1102 22-23 0.6287202794863234 1102 24-25 0.7518196812126732 1102 26-27 0.5754377824262349 1102 28-29 0.784814396317131 1102 30-31 0.9341609270648732 1102 32-33 1.007476649818237 1102 34-35 0.856154611776347 1102 36-37 0.9103345383202281 1102 38-39 0.8708883260952618 1102 40-41 0.8213401604197301 1102 42-43 0.8707557042358545 1102 44-45 0.9086275315490226 1102 46-47 1.102672703563627 1102 48-49 0.908219361252641 1102 50-51 0.8901272807348093 1102 52-53 0.8562610902252175 1102 54-55 1.0283397895219295 1102 56-57 1.12652814335636 1102 58-59 0.8943932582563221 1102 60-61 1.0396357001444727 1102 62-63 0.9546949085113496 1102 64-65 1.089960421012755 1102 66-67 1.072593778664448 1102 68-69 0.9233166732444218 1102 70-71 1.018740039575114 1102 72-73 0.9742100475682776 1102 74-75 1.0322880750248196 1102 76-77 1.0789876219355357 1102 78-79 0.9837003022216741 1102 80-81 1.0404868161868244 1102 82-83 1.0877694826904722 1102 84-85 1.172821311449308 1102 86-87 1.3566722660924775 1102 88-89 1.3277327812726014 1102 90-91 1.1825115260888701 1102 92-93 1.2599418494554158 1102 94-95 1.4206290609382464 1102 96-97 1.2627264198987866 1102 98-99 1.3452779938009236 1102 100-101 1.4483764869598197 1103 1 0.3914221479845388 1103 2 0.5169065971136426 1103 3 0.46485073791663467 1103 4 0.4386490358606778 1103 5 0.2827362360616519 1103 6 0.24428126578123255 1103 7 0.4207464757066717 1103 8 0.4158897125208867 1103 9 0.588808968520496 1103 10-11 0.5343129208916793 1103 12-13 0.43897046149388075 1103 14-15 0.5279718605484405 1103 16-17 0.6368101923172347 1103 18-19 0.5771114570054081 1103 20-21 0.5674075147743807 1103 22-23 0.6981661273194675 1103 24-25 0.7541463874077508 1103 26-27 0.6787427964982911 1103 28-29 0.7569404022100201 1103 30-31 0.7393063563064644 1103 32-33 0.7793691767397277 1103 34-35 0.7334400583436036 1103 36-37 0.7922164758319283 1103 38-39 0.7310397438771119 1103 40-41 0.9261416359313799 1103 42-43 0.7739407454146416 1103 44-45 0.8067718250562095 1103 46-47 0.7274805515826941 1103 48-49 0.6441755943867165 1103 50-51 0.607320992834893 1103 52-53 0.7654390502414401 1103 54-55 0.7450164932245826 1103 56-57 0.7705552312282506 1103 58-59 0.7318155049087309 1103 60-61 0.7194879334186552 1103 62-63 0.8419571014892 1103 64-65 0.806235306462554 1103 66-67 0.6907869505160136 1103 68-69 0.8691089321972569 1103 70-71 0.9132814413381354 1103 72-73 0.8837039384488605 1103 74-75 0.9446980953548696 1103 76-77 0.8870333504020529 1103 78-79 0.8655859446795482 1103 80-81 1.0995396163851403 1103 82-83 1.0537803065223024 1103 84-85 1.0583681143502162 1103 86-87 1.214248072659263 1103 88-89 1.1001864391322727 1103 90-91 1.0904520340841444 1103 92-93 1.187077346746765 1103 94-95 1.0585941832486263 1103 96-97 0.9642990685041788 1103 98-99 1.112256751660464 1103 100-101 1.3798774212798826 1104 1 0.25139385579684514 1104 2 0.3982649313273434 1104 3 0.34444274292574306 1104 4 0.3566351886642565 1104 5 0.2664643630827541 1104 6 0.2818470038903982 1104 7 0.4450344685331302 1104 8 0.3736768923978744 1104 9 0.4385022121502402 1104 10-11 0.4913212925963961 1104 12-13 0.47859434030643655 1104 14-15 0.5566228539958828 1104 16-17 0.48815574608913437 1104 18-19 0.5095915584243969 1104 20-21 0.412197938719153 1104 22-23 0.5295944189918274 1104 24-25 0.4809702414839663 1104 26-27 0.5075576681914171 1104 28-29 0.5230879493185796 1104 30-31 0.6020982644315751 1104 32-33 0.6314149492736263 1104 34-35 0.6145233296213704 1104 36-37 0.6826261199120864 1104 38-39 0.5346525377177684 1104 40-41 0.5542720067445615 1104 42-43 0.6698522923892085 1104 44-45 0.586830341958084 1104 46-47 0.49458276677339086 1104 48-49 0.2585283397338358 1104 50-51 0.433589438800162 1104 52-53 0.5616025986692605 1104 54-55 0.5060911583177443 1104 56-57 0.5236028254904497 1104 58-59 0.38446577172621943 1104 60-61 0.30172788621347735 1104 62-63 0.36677975997569234 1104 64-65 0.34525752536369225 1104 66-67 0.36950055782147473 1104 68-69 0.37594041331131933 1104 70-71 0.2704587158573801 1104 72-73 0.4631030773150542 1104 74-75 0.4678942107189705 1104 76-77 0.5580522814314683 1104 78-79 0.5788995061197149 1104 80-81 0.5665372174756023 1104 82-83 0.5523998384386211 1104 84-85 0.6414751273599819 1104 86-87 0.595163214133084 1104 88-89 0.5853852188062163 1104 90-91 0.7219218106583156 1104 92-93 0.5762192534546919 1104 94-95 0.4377887319779248 1104 96-97 0.5900569409728895 1104 98-99 0.7331401481659405 1104 100-101 0.8069838107277754 1105 1 0.36540791581274235 1105 2 0.4020121988154237 1105 3 0.45268375047741216 1105 4 0.4184023786483593 1105 5 0.3298188940843403 1105 6 0.2807999550032747 1105 7 0.1914983496937026 1105 8 0.18786980773968764 1105 9 0.2948652387798134 1105 10-11 0.3560954877474245 1105 12-13 0.34469815091851785 1105 14-15 0.3225999754187754 1105 16-17 0.5490063025279213 1105 18-19 0.4310423980507849 1105 20-21 0.48795869487972965 1105 22-23 0.3998292908121712 1105 24-25 0.57461653023595 1105 26-27 0.4565702377462699 1105 28-29 0.4055545381500565 1105 30-31 0.2753103321978756 1105 32-33 0.32800800372513805 1105 34-35 0.35556006948623065 1105 36-37 0.24499073636677338 1105 38-39 0.4632488960246022 1105 40-41 0.49504294772230395 1105 42-43 0.49830954099811464 1105 44-45 0.421401585996243 1105 46-47 0.49371333116130245 1105 48-49 0.7259257463316047 1105 50-51 0.6279753688478635 1105 52-53 0.5967669219999507 1105 54-55 0.7062426885244193 1105 56-57 0.6111593388052299 1105 58-59 0.6285461572588176 1105 60-61 0.4679925424137963 1105 62-63 0.4894322838230707 1105 64-65 0.5490457805306264 1105 66-67 0.7039314501108294 1105 68-69 0.9853955255927147 1105 70-71 0.7489954557222518 1105 72-73 0.5825213006854852 1105 74-75 0.9351705173564859 1105 76-77 0.6282287768607198 1105 78-79 0.6423447068351393 1105 80-81 0.7531274152100984 1105 82-83 0.7242593128026904 1105 84-85 0.8021909212788998 1105 86-87 0.8584146549120959 1105 88-89 0.7821683070415126 1105 90-91 0.7238469895136426 1105 92-93 0.8257447900206678 1105 94-95 0.8413490708094038 1105 96-97 0.7890502587391879 1105 98-99 0.8434833268225397 1105 100-101 1.1538290015067894 1106 1 0.4933520018925357 1106 2 0.5166662263522817 1106 3 0.6505378539032591 1106 4 0.5557004597411606 1106 5 0.5297755289499122 1106 6 0.5476249495826337 1106 7 0.5351257076728899 1106 8 0.5315445963346619 1106 9 0.454736905085106 1106 10-11 0.6342428478948676 1106 12-13 0.6502275443486951 1106 14-15 0.8825187335497375 1106 16-17 0.827041861938163 1106 18-19 0.9753530956873888 1106 20-21 0.9777878769048769 1106 22-23 0.9334985910073144 1106 24-25 1.0820085917060212 1106 26-27 1.073145882802642 1106 28-29 0.9611936995767678 1106 30-31 0.9482623459662989 1106 32-33 0.9031130286600941 1106 34-35 0.7606110912772124 1106 36-37 0.9527591936521205 1106 38-39 0.8174976628285933 1106 40-41 0.8011333775791982 1106 42-43 0.8918362783138107 1106 44-45 1.0153616358661495 1106 46-47 0.943070985107525 1106 48-49 0.927230088265695 1106 50-51 0.9489261494202808 1106 52-53 1.1128489498620482 1106 54-55 1.1188063810656175 1106 56-57 1.2851893827124314 1106 58-59 1.3235415497132763 1106 60-61 1.3373476215551676 1106 62-63 1.3115878020364278 1106 64-65 1.133866628319005 1106 66-67 1.3589150526693672 1106 68-69 1.087827699812145 1106 70-71 1.2127303456832195 1106 72-73 1.2885856437036978 1106 74-75 1.147605808444954 1106 76-77 1.2028042728494448 1106 78-79 1.2350126112150157 1106 80-81 1.384986221042709 1106 82-83 1.2424608116101474 1106 84-85 1.4136030662268624 1106 86-87 1.415552962587725 1106 88-89 1.3804594497128058 1106 90-91 1.5648121347868447 1106 92-93 1.5955667219374092 1106 94-95 1.7267184197903198 1106 96-97 1.586914864658528 1106 98-99 1.7445284943203667 1106 100-101 1.9864368722786843 1107 1 0.1852417376735609 1107 2 0.303400956379722 1107 3 0.39949374480418953 1107 4 0.4288387440038832 1107 5 0.24411541450794516 1107 6 0.24272672315758825 1107 7 0.2997422509796337 1107 8 0.3772762468471029 1107 9 0.35987682160885726 1107 10-11 0.48768706301218145 1107 12-13 0.3992113175221448 1107 14-15 0.18517620934464674 1107 16-17 0.3951106898199086 1107 18-19 0.4491890272111547 1107 20-21 0.4586606379583955 1107 22-23 0.3980664551465125 1107 24-25 0.45303405576544975 1107 26-27 0.4573964007568563 1107 28-29 0.32815819265081103 1107 30-31 0.5817961255034731 1107 32-33 0.5997447189293794 1107 34-35 0.6265137618084751 1107 36-37 0.6357587488478629 1107 38-39 0.7424854930367033 1107 40-41 0.8297140190158032 1107 42-43 0.9161807824882828 1107 44-45 1.0943284959811095 1107 46-47 1.045221462780063 1107 48-49 0.993372087103161 1107 50-51 0.7933781676375773 1107 52-53 0.7897232618259693 1107 54-55 0.9388518980554394 1107 56-57 0.7827016279504662 1107 58-59 0.9047540808594192 1107 60-61 1.0822051464228721 1107 62-63 1.0096313193752664 1107 64-65 1.0852291201675328 1107 66-67 1.0194654421683182 1107 68-69 1.1308421736638223 1107 70-71 1.1765489967585623 1107 72-73 1.102621054845855 1107 74-75 1.096359175639396 1107 76-77 1.0306440331012645 1107 78-79 1.241993676199737 1107 80-81 1.225816168046709 1107 82-83 1.2824482547467149 1107 84-85 1.3145544093651367 1107 86-87 1.348792633353849 1107 88-89 1.4282703304908324 1107 90-91 1.3543902005575141 1107 92-93 1.3972250282960097 1107 94-95 1.4489860554917016 1107 96-97 1.3730830925137703 1107 98-99 1.339257367102416 1107 100-101 1.5942743494644276 1108 1 -0.004194461206346034 1108 2 0.21440550828678795 1108 3 0.30342828975788194 1108 4 0.17610015764982734 1108 5 0.10713166941474128 1108 6 0.2724562967800708 1108 7 0.17768938310045712 1108 8 4.076656692078018E-4 1108 9 -0.024351675684208374 1108 10-11 0.07057442497209365 1108 12-13 -0.050248962701161304 1108 14-15 0.04637303433948148 1108 16-17 0.2682060088421707 1108 18-19 0.34631348377032367 1108 20-21 0.30601291202363257 1108 22-23 0.277213766766657 1108 24-25 0.5354754463519527 1108 26-27 0.37291453840706623 1108 28-29 0.2548644913438807 1108 30-31 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2316 98-99 -2.840489643067972 2316 100-101 -2.7584200860844525 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3800.0 3 1122.0 4 1169.0 5 1212.0 6 1457.0 7 1633.0 8 1971.0 9 2466.0 10 3070.0 11 3818.0 12 4666.0 13 5730.0 14 6374.0 15 7718.0 16 9354.0 17 12271.0 18 10810.0 19 7556.0 20 7163.0 21 7804.0 22 8348.0 23 9258.0 24 10601.0 25 12119.0 26 14115.0 27 16763.0 28 20130.0 29 23880.0 30 29490.0 31 36475.0 32 45906.0 33 59357.0 34 80709.0 35 112813.0 36 166386.0 37 242875.0 38 222701.0 39 41042.0 40 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.66974087311718 19.30503255042124 14.778768828184836 22.246457748276743 2 15.110947589704216 20.749988627103885 40.43156349787187 23.707500285320023 3 19.13255984285516 25.330669572973896 30.173468882991877 25.363301701179065 4 12.487794986104447 17.160389251125096 38.095770338962076 32.256045423808374 5 13.444427062215256 37.77604487594942 34.11063824352929 14.668889818306042 6 30.62806227547969 36.883863589020784 17.94829637918223 14.539777756317296 7 27.601117196249298 33.36645968682425 21.144591344522855 17.887831772403594 8 25.904291869812933 34.71101686596217 20.09134898168152 19.293342282543374 9 26.051472648420305 17.240647123951202 20.958089568251427 35.74979065937706 10-11 24.365790776009323 27.153581520090675 27.660677511533983 20.819950192366022 12-13 25.69914059666222 24.713562148424547 28.01442594115579 21.572871313757435 14-15 22.685229339163698 25.74038462689563 26.87615649245938 24.698229541481293 16-17 21.57752277687048 29.165648951234267 27.205129924345826 22.05169834754942 18-19 21.419858433930848 27.61775325813841 29.871166816766547 21.09122149116419 20-21 22.39493255360344 26.58885326164797 29.85602684849725 21.160187336251333 22-23 22.05111223266419 26.66473830465061 29.797462142596387 21.48668732008881 24-25 21.966989458567284 26.781222885978995 29.87986556432069 21.37192209113303 26-27 21.722918301270173 26.856068598899313 29.87392395389032 21.547089145940195 28-29 21.421621252867908 27.132745050400597 29.939795349668497 21.505838347062994 30-31 21.688320391091313 26.771933071563392 29.852622778452325 21.68712375889297 32-33 21.470076279123194 26.915394151348888 29.867002139516924 21.747527430010994 34-35 22.01054170314516 26.959199261322958 29.585291871162276 21.4449671643696 36-37 21.970601996536953 26.650541415158347 29.643432237446454 21.735424350858242 38-39 21.676119467877562 27.063478229305982 29.662583061861962 21.59781924095449 40-41 21.913379911681197 26.912061393097662 29.650949632590105 21.52360906263103 42-43 21.697806862172524 27.003208964035984 29.327633472798002 21.971350700993487 44-45 21.874810534142146 27.126524900999105 29.11587652091224 21.882788043946505 46-47 22.152099661954665 27.273909249424495 28.81369857331787 21.76029251530297 48-49 22.11299379143596 27.415720119241442 28.522819643727498 21.948466445595095 50-51 21.946538173033325 27.579925355546152 28.503270629812082 21.97026584160844 52-53 22.23127162500374 27.792320194637497 28.122007398617992 21.854400781740768 54-55 21.912653485819312 27.56901277665739 28.380517192964543 22.137816544558753 56-57 22.09070460671096 27.897667824902783 28.125671876003448 21.885955692382804 58-59 22.349418521389232 27.527935027898128 28.15096064742327 21.971685803289372 60-61 22.154559514041164 27.522628734773395 28.570704848538757 21.752106902646677 62-63 21.99820400679621 27.60475862401868 28.590480438155318 21.806556931029792 64-65 21.93293485969 27.641815642257562 28.317939211098764 22.10731028695367 66-67 21.912175607296334 27.844554798291288 28.05614907982019 22.187120514592188 68-69 21.844698535078496 27.705222243319618 28.282909674609037 22.167169546992852 70-71 21.982663567371585 27.65510731688059 28.239873954556167 22.122355161191656 72-73 22.045601358949206 27.577338086465534 28.270689283919896 22.10637127066536 74-75 21.826990105106326 28.034428742568462 27.963602806876498 22.174978345448714 76-77 21.98493163797642 27.81576554298751 27.965571704344217 22.233731114691853 78-79 22.02446253824765 27.90133602479764 27.895471717683478 22.17872971927123 80-81 22.068811483337555 28.027288481365304 27.767844501123722 22.13605553417342 82-83 22.104716018474416 27.978696498908178 27.95707546379514 21.95951201882227 84-85 22.047466806666034 27.945551841919862 27.80556577270794 22.20141557870616 86-87 22.078499238433082 27.82990528485156 27.952138969806207 22.139456506909156 88-89 22.11724126648053 27.714466874934907 27.77468084401709 22.393611014567472 90-91 21.981750821683754 27.80169327290432 28.009772042846038 22.20678386256589 92-93 22.155470264835664 27.864398394548484 27.860887466785826 22.119243873830026 94-95 21.978571388670094 27.665947644664275 27.88197345603791 22.47350751062772 96-97 21.868251315619357 27.727565836832873 28.026483174277868 22.377699673269902 98-99 21.786914750812187 27.844164262742794 27.712003333760432 22.65691765268459 100-101 21.860474955605376 27.75820427393787 27.811449881075497 22.569870889381253 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 417.0 1 300.5 2 238.0 3 309.5 4 300.5 5 317.5 6 476.0 7 957.0 8 1680.5 9 2036.0 10 1771.0 11 1504.5 12 1512.0 13 1616.5 14 1736.0 15 1781.0 16 1849.5 17 1971.0 18 2106.5 19 2252.0 20 2534.0 21 2967.5 22 3478.0 23 4125.5 24 4999.5 25 6163.0 26 7731.0 27 9771.0 28 11516.5 29 13327.0 30 15673.5 31 18142.0 32 20761.5 33 23256.0 34 26093.5 35 28881.5 36 32005.5 37 35361.5 38 37258.0 39 38237.5 40 39167.5 41 40823.0 42 42309.0 43 43652.0 44 45238.5 45 46249.5 46 46136.5 47 47423.5 48 51919.5 49 51735.5 50 46829.5 51 43469.5 52 41203.5 53 39703.0 54 37713.5 55 34600.5 56 31394.5 57 28133.0 58 25368.0 59 22921.0 60 19655.0 61 15612.5 62 12025.5 63 9207.5 64 6916.5 65 5136.0 66 3637.5 67 2661.5 68 1889.0 69 1225.5 70 858.5 71 616.0 72 438.5 73 324.5 74 248.0 75 187.5 76 116.0 77 55.0 78 33.5 79 20.5 80 15.5 81 13.5 82 12.0 83 11.5 84 8.5 85 7.0 86 6.5 87 5.5 88 6.0 89 4.0 90 2.0 91 2.0 92 1.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.057408943037570964 2 0.09416661351023792 3 0.06330930662754354 4 0.12765516361548765 5 0.10237928175033488 6 0.09655865280347005 7 0.11011354213178541 8 0.09273138993429865 9 0.11298398928366396 10-11 0.10684442176436819 12-13 0.09085762582126683 14-15 0.09404701154557632 16-17 0.10796070676787652 18-19 0.08814664795560374 20-21 0.07219971933405625 22-23 0.07475122791350386 24-25 0.06159501180072718 26-27 0.05346207820373796 28-29 0.06789404860623845 30-31 0.05118964087516744 32-33 0.0483191937232889 34-35 0.045249409963641005 36-37 0.050990304267398104 38-39 0.051947119984690954 40-41 0.06853192575110034 42-43 0.06346877591375902 44-45 0.050711233016521015 46-47 0.03843209797792945 48-49 0.04536901192830261 50-51 0.02786725776615424 52-53 0.04365471710148625 54-55 0.049754417299228165 56-57 0.05370128213306117 58-59 0.060638196083434334 60-61 0.05744881035912484 62-63 0.06494386681125215 64-65 0.04329591120750144 66-67 0.06522293806212924 68-69 0.05693053517892454 70-71 0.026033360974676276 72-73 0.019933660776934363 74-75 0.030458633667155705 76-77 0.04305670727817822 78-79 0.06498373413280602 80-81 0.04126267780825413 82-83 0.05988071697391082 84-85 0.06546214199145245 86-87 0.06562161127766793 88-89 0.08998054474708171 90-91 0.06287076609045097 92-93 0.07415321809019583 94-95 0.08523633348217133 96-97 0.09073802385660522 98-99 0.1212365248453148 100-101 0.11756873126235887 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1254160.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.30139749325687 #Duplication Level Percentage of deduplicated Percentage of total 1 83.6450140793065 55.45781326801169 2 10.258812788904798 13.603472490521684 3 2.6709574168116488 5.312646281387751 4 1.1200117921868051 2.9703338812364954 5 0.5799287314585382 1.9225042671096384 6 0.3523151518941503 1.4015392157178739 7 0.24530692941618423 1.1384934564550917 8 0.1707833462043185 0.9058539617536819 9 0.12288086562571184 0.7332455804549224 >10 0.7508359324855765 9.21656681398426 >50 0.05583777006706123 2.545568389732645 >100 0.025630571118361672 2.9439225905334685 >500 9.626425830739181E-4 0.42778403661830083 >1k 6.016516144211988E-4 0.9469169583406588 >5k 1.2033032288423977E-4 0.47333880814180873 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5933 0.47306563755820624 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4411 0.35170951074822987 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3037 0.24215411111819865 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2021 0.16114371372073738 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.973464310773745E-5 0.0 10-11 0.0 0.0 0.0 7.973464310773745E-5 0.0 12-13 0.0 0.0 0.0 7.973464310773745E-5 0.0 14-15 7.973464310773745E-5 0.0 0.0 7.973464310773745E-5 0.0 16-17 7.973464310773745E-5 0.0 0.0 7.973464310773745E-5 0.0 18-19 7.973464310773745E-5 0.0 0.0 7.973464310773745E-5 0.0 20-21 7.973464310773745E-5 0.0 0.0 1.1960196466160618E-4 0.0 22-23 7.973464310773745E-5 0.0 0.0 2.790712508770811E-4 0.0 24-25 7.973464310773745E-5 0.0 0.0 5.182751802002934E-4 0.0 26-27 7.973464310773745E-5 0.0 0.0 7.973464310773745E-4 0.0 28-29 7.973464310773745E-5 0.0 0.0 0.0014750908974931428 0.0 30-31 7.973464310773745E-5 0.0 0.0 0.0028305798303246794 0.0 32-33 7.973464310773745E-5 0.0 0.0 0.004704343943356509 0.0 34-35 1.594692862154749E-4 0.0 0.0 0.006777444664157683 0.0 36-37 2.3920392932321236E-4 3.9867321553868726E-5 0.0 0.009289085922051413 0.0 38-39 2.3920392932321236E-4 7.973464310773745E-5 0.0 0.011122982713529374 0.0 40-41 2.3920392932321236E-4 7.973464310773745E-5 0.0 0.014152899151623398 0.0 42-43 2.3920392932321236E-4 7.973464310773745E-5 0.0 0.018976845059641513 0.0 44-45 2.3920392932321236E-4 7.973464310773745E-5 0.0 0.026471901511768835 0.0 46-47 2.3920392932321236E-4 7.973464310773745E-5 0.0 0.04138227977291574 0.0 48-49 2.3920392932321236E-4 7.973464310773745E-5 0.0 0.07192064808317919 0.0 50-51 2.3920392932321236E-4 7.973464310773745E-5 0.0 0.09241245136186771 0.0 52-53 2.3920392932321236E-4 7.973464310773745E-5 0.0 0.11051221534732411 0.0 54-55 2.3920392932321236E-4 7.973464310773745E-5 0.0 0.13283791541749057 0.0 56-57 2.3920392932321236E-4 7.973464310773745E-5 0.0 0.1526918415513172 0.0 58-59 2.3920392932321236E-4 7.973464310773745E-5 0.0 0.17764878484403904 0.0 60-61 2.3920392932321236E-4 1.594692862154749E-4 0.0 0.21588154621419914 0.0 62-63 2.3920392932321236E-4 1.594692862154749E-4 0.0 0.24482522166230786 0.0 64-65 2.3920392932321236E-4 2.3920392932321236E-4 0.0 0.2811443515978822 0.0 66-67 2.3920392932321236E-4 2.3920392932321236E-4 0.0 0.34401511768833326 0.0 68-69 2.3920392932321236E-4 2.3920392932321236E-4 0.0 0.41613510237928175 0.0 70-71 2.3920392932321236E-4 2.3920392932321236E-4 0.0 0.47761051221534734 0.0 72-73 2.3920392932321236E-4 2.3920392932321236E-4 0.0 0.5449862856413855 0.0 74-75 2.3920392932321236E-4 2.3920392932321236E-4 0.0 0.6035115136824647 0.0 76-77 2.3920392932321236E-4 2.3920392932321236E-4 0.0 0.6514320341902149 0.0 78-79 2.3920392932321236E-4 2.3920392932321236E-4 0.0 0.7070469477578618 0.0 80-81 2.3920392932321236E-4 2.3920392932321236E-4 0.0 0.8089876889711042 0.0 82-83 2.3920392932321236E-4 2.3920392932321236E-4 0.0 0.9242042482617847 7.973464310773745E-5 84-85 2.3920392932321236E-4 2.3920392932321236E-4 0.0 1.0455603750717612 7.973464310773745E-5 86-87 2.3920392932321236E-4 2.3920392932321236E-4 0.0 1.2438205651591505 1.1960196466160618E-4 88-89 2.3920392932321236E-4 2.3920392932321236E-4 0.0 1.4848982585953947 1.594692862154749E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7625 0.0 30.422235 1 GGTATCA 5185 0.0 26.769808 1 GTACATG 5495 0.0 25.346096 1 TATCAAC 9080 0.0 25.338911 2 ATCAACG 9110 0.0 25.150103 3 CAACGCA 9140 0.0 25.065552 5 GTACCGT 95 0.0012225374 25.014286 6 TCAACGC 9165 0.0 24.94731 4 AACGCAG 9380 0.0 24.624939 6 TACATGG 5570 0.0 24.493744 2 ACATGGG 5600 0.0 23.34295 3 GGTATAC 170 3.1294367E-6 22.36929 3 GTGTAGC 605 0.0 21.99955 1 GAGTACT 4750 0.0 21.85943 12-13 ACGCAGA 10495 0.0 21.78233 7 AGAGTAC 7835 0.0 21.444609 10-11 CGCAGAG 10560 0.0 21.424932 8 AGTACTT 5030 0.0 21.114975 12-13 GCAGAGT 10880 0.0 20.53431 9 GTACTTT 5245 0.0 19.705044 14-15 >>END_MODULE