FastQCFastQC Report
Thu 26 May 2016
SRR936875_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936875_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254160
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64400.5134911016138292No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54140.43168335778529054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34670.27644000765452575No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24410.1946322638259871No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14340.11433947821649551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85350.028.8922651
GGTATCA59950.027.7393721
TCAACGC103300.023.6792964
ATCAACG103350.023.6697273
GTACATG50750.023.4993291
CAACGCA104450.023.4185875
GAGTACT58050.023.3596212-13
TATCAAC105000.023.3430122
AACGCAG106650.023.0245726
AGAGTAC85950.022.18715310-11
TACATGG50750.022.1824172
AGTACTT61650.021.64886512-13
ATTCGCG550.002879191421.58976480-81
GTACTTT62650.021.56865514-15
ACATGGG50900.021.4637993
ACGCAGA117050.020.8976617
CGCAGAG117250.020.8215088
CATGGGA29250.019.9728984
GCAGAGT121850.019.5677159
TACTTTT69650.018.923614-15
Produced by FastQC (version 0.11.5)