##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936875_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1254160 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07603336097468 31.0 31.0 34.0 31.0 34.0 2 32.071977259679784 33.0 31.0 34.0 30.0 34.0 3 32.25120797984308 33.0 31.0 34.0 30.0 34.0 4 35.997152675894625 37.0 35.0 37.0 35.0 37.0 5 35.922796931810936 37.0 35.0 37.0 35.0 37.0 6 35.96469828411048 37.0 35.0 37.0 35.0 37.0 7 35.90491404605473 37.0 35.0 37.0 35.0 37.0 8 35.881391528991514 37.0 35.0 37.0 35.0 37.0 9 37.550164253364805 39.0 37.0 39.0 35.0 39.0 10-11 37.47936108630478 39.0 37.0 39.0 35.0 39.0 12-13 37.383272469222426 39.0 37.0 39.0 34.0 39.0 14-15 38.56523928366397 40.0 38.0 41.0 34.0 41.0 16-17 38.48108335459591 40.0 38.0 41.0 34.0 41.0 18-19 38.55151097148689 40.0 38.0 41.0 34.0 41.0 20-21 38.64583785162978 40.0 38.0 41.0 34.0 41.0 22-23 38.57618166741086 40.0 38.0 41.0 34.0 41.0 24-25 38.48150315749187 40.0 38.0 41.0 34.0 41.0 26-27 38.326122265101745 40.0 38.0 41.0 34.0 41.0 28-29 38.15158990878356 40.0 38.0 41.0 33.5 41.0 30-31 38.01676819544556 40.0 37.0 41.0 33.0 41.0 32-33 37.982500637877145 40.0 37.0 41.0 33.0 41.0 34-35 37.892817902022074 40.0 37.0 41.0 33.0 41.0 36-37 37.73358502902341 40.0 37.0 41.0 33.0 41.0 38-39 37.58210555272055 40.0 37.0 41.0 33.0 41.0 40-41 37.3971957326019 40.0 36.5 41.0 32.5 41.0 42-43 37.168638371499654 40.0 36.0 41.0 31.5 41.0 44-45 36.84425551763731 39.0 36.0 41.0 31.0 41.0 46-47 36.55436786374945 39.0 36.0 41.0 30.5 41.0 48-49 36.22238908911144 39.0 35.5 41.0 30.0 41.0 50-51 35.959495997320914 39.0 35.0 40.0 29.0 41.0 52-53 35.70399151623397 39.0 35.0 40.0 28.0 41.0 54-55 35.467390923008224 38.5 35.0 40.0 28.0 41.0 56-57 35.20130525610767 38.0 34.5 40.0 27.5 41.0 58-59 34.87550073355872 38.0 34.0 40.0 26.5 41.0 60-61 34.55350393889137 38.0 34.0 40.0 26.0 41.0 62-63 34.19451904063277 37.0 33.5 40.0 26.0 41.0 64-65 33.78718983223831 36.5 33.0 39.0 25.0 41.0 66-67 33.406985552082666 36.0 33.0 39.0 24.0 40.0 68-69 32.97461209096128 35.5 32.0 39.0 22.5 40.0 70-71 32.484272740320215 35.0 31.5 38.0 22.0 40.0 72-73 32.138160601518145 35.0 31.0 37.5 21.5 39.5 74-75 31.693929004273777 35.0 31.0 37.0 20.0 39.0 76-77 30.297306962429037 33.5 29.5 35.5 19.5 38.0 78-79 31.027053167060025 34.0 31.0 36.0 20.0 38.5 80-81 30.839937886713017 34.0 31.0 36.0 18.0 37.5 82-83 30.4996228551381 34.0 30.5 35.0 17.5 37.0 84-85 30.100174618868408 34.0 30.0 35.0 13.0 36.5 86-87 29.73186993685016 34.0 30.0 35.0 7.5 36.0 88-89 29.36296405562289 34.0 29.0 35.0 4.5 36.0 90-91 28.924191490718886 34.0 29.0 35.0 2.0 35.0 92-93 28.448056866747464 34.0 29.0 35.0 2.0 35.0 94-95 27.93818731262359 33.0 28.0 35.0 2.0 35.0 96-97 27.294374322255536 33.0 27.0 35.0 2.0 35.0 98-99 26.515061475409837 33.0 25.5 34.5 2.0 35.0 100-101 23.40344772596798 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4008181935846622 1101 2 0.2910904102299696 1101 3 0.3133966702103237 1101 4 0.29420507829594555 1101 5 0.3976138841249295 1101 6 0.2662112513002839 1101 7 0.3670008219881282 1101 8 0.4096881606897256 1101 9 0.373697245317544 1101 10-11 0.46040830496392715 1101 12-13 0.47309022091989306 1101 14-15 0.581987034539388 1101 16-17 0.7182241976893948 1101 18-19 0.6805884793315116 1101 20-21 0.6050168187366012 1101 22-23 0.5372313786918212 1101 24-25 0.6791288939631315 1101 26-27 0.7438603009159905 1101 28-29 0.8303980523822858 1101 30-31 0.758267097400001 1101 32-33 0.7200929695695848 1101 34-35 0.8362034686297122 1101 36-37 0.8805124407293548 1101 38-39 0.9413315781356104 1101 40-41 0.8525588679975229 1101 42-43 0.9620273077426873 1101 44-45 1.1903745678592657 1101 46-47 1.170338023255347 1101 48-49 1.009366346562274 1101 50-51 0.8740177891831635 1101 52-53 0.6980687712436335 1101 54-55 0.7127672414949657 1101 56-57 0.9010239652826968 1101 58-59 1.1089427893347974 1101 60-61 1.0546546649994113 1101 62-63 0.8735290500268249 1101 64-65 0.7940195530851568 1101 66-67 0.9530119034742981 1101 68-69 1.0206605700887579 1101 70-71 0.9935967389016724 1101 72-73 1.034997379276767 1101 74-75 1.0317470455288529 1101 76-77 0.8481204204086552 1101 78-79 1.0267765370927648 1101 80-81 1.2836171899126292 1101 82-83 1.538569739904208 1101 84-85 1.2744314088269348 1101 86-87 1.0915137063466958 1101 88-89 1.0888424777699655 1101 90-91 1.1504576730371667 1101 92-93 1.1867648904266623 1101 94-95 1.447381079375301 1101 96-97 1.3613430476637767 1101 98-99 1.439547806545061 1101 100-101 1.5568116927417535 1102 1 0.35503521513335556 1102 2 0.23132103026108553 1102 3 0.26788011970624126 1102 4 0.295142904670179 1102 5 0.33545730974890375 1102 6 0.30702801281530157 1102 7 0.3195041789802673 1102 8 0.33602403041711426 1102 9 0.45366399510973565 1102 10-11 0.4715385988872427 1102 12-13 0.4376084126201292 1102 14-15 0.592969194524045 1102 16-17 0.6076363510666454 1102 18-19 0.5922036721187709 1102 20-21 0.48966204328208107 1102 22-23 0.5311568214950739 1102 24-25 0.4248873610432895 1102 26-27 0.5274621936201314 1102 28-29 0.5107218156237607 1102 30-31 0.5092496836693741 1102 32-33 0.6743313053540518 1102 34-35 0.6245648301830968 1102 36-37 0.6789170701813632 1102 38-39 0.5820641484193061 1102 40-41 0.6515038133265065 1102 42-43 0.6937205106329003 1102 44-45 0.883172487589853 1102 46-47 0.7203817174386913 1102 48-49 0.6402869084055283 1102 50-51 0.7776859691605651 1102 52-53 0.7090775740230129 1102 54-55 0.6526525709064757 1102 56-57 0.5220206189476784 1102 58-59 0.709179377351937 1102 60-61 0.6128212144377798 1102 62-63 0.6140985664068168 1102 64-65 0.6154307686360241 1102 66-67 0.5966250927234 1102 68-69 0.4321734545442837 1102 70-71 0.5356284118560382 1102 72-73 0.5900882844923601 1102 74-75 0.5607035005451593 1102 76-77 0.5679874195773991 1102 78-79 0.6722909561732671 1102 80-81 0.727164305030815 1102 82-83 0.8448744908473635 1102 84-85 0.61853482549893 1102 86-87 0.6255546723078602 1102 88-89 0.6937756576408027 1102 90-91 0.8073101998398187 1102 92-93 0.6454440472354968 1102 94-95 0.8243073534337455 1102 96-97 0.84239469205707 1102 98-99 1.0289503685162025 1102 100-101 1.51927732353656 1103 1 0.06760953298540073 1103 2 -0.1846662840032245 1103 3 -0.028075022152776796 1103 4 0.09878749389942953 1103 5 0.24696904903925088 1103 6 0.18491770242165018 1103 7 0.23513447115905706 1103 8 0.302189788361531 1103 9 0.30341091360495653 1103 10-11 0.3738320952920944 1103 12-13 0.3279275230019252 1103 14-15 0.2957176583186367 1103 16-17 0.3684285224480206 1103 18-19 0.3502161234324106 1103 20-21 0.3392048529246381 1103 22-23 0.419820831378523 1103 24-25 0.4632857599676399 1103 26-27 0.42010598931727117 1103 28-29 0.3821510895486142 1103 30-31 0.39190864943702053 1103 32-33 0.4687354220207922 1103 34-35 0.2922473257270539 1103 36-37 0.29802064835100595 1103 38-39 0.43620614351261366 1103 40-41 0.2935948984176733 1103 42-43 0.29364223522471633 1103 44-45 0.4431346836663792 1103 46-47 0.3427992015512089 1103 48-49 0.17423634060568816 1103 50-51 0.09637230302875821 1103 52-53 0.2833934051906368 1103 54-55 0.3961116568491079 1103 56-57 0.1358914658714312 1103 58-59 0.2589947363073364 1103 60-61 0.20024817647622228 1103 62-63 0.11742493444134539 1103 64-65 -0.03619280613855125 1103 66-67 0.07152441923154385 1103 68-69 -0.059917495821558475 1103 70-71 -0.0375264239070674 1103 72-73 0.12283832457313792 1103 74-75 0.13803886284173927 1103 76-77 0.10933057708072269 1103 78-79 0.27493345850738393 1103 80-81 0.4832778030254268 1103 82-83 0.5240079645644755 1103 84-85 0.38070591048230895 1103 86-87 0.21877617015697481 1103 88-89 0.3504827553745855 1103 90-91 0.578786088723767 1103 92-93 0.5063004152296102 1103 94-95 0.5548489761462783 1103 96-97 0.9842772897228187 1103 98-99 1.2746438738129235 1103 100-101 1.275726624379537 1104 1 0.24034776840835548 1104 2 0.13390426480852824 1104 3 0.11465494205683058 1104 4 0.14291351523967677 1104 5 0.05839955667530461 1104 6 0.026895011984045425 1104 7 0.20692989132896145 1104 8 0.18818747448315776 1104 9 0.10252607414637538 1104 10-11 0.22196579005082384 1104 12-13 0.2815224087638484 1104 14-15 0.30646830313830975 1104 16-17 0.26531019771657327 1104 18-19 0.31542807005344997 1104 20-21 0.27004746023783355 1104 22-23 0.30062259796539337 1104 24-25 0.25615048569060406 1104 26-27 0.2480692610073092 1104 28-29 0.41371440040452256 1104 30-31 0.4540690079077976 1104 32-33 0.36333349516304736 1104 34-35 0.3375785994166094 1104 36-37 0.24520405542096313 1104 38-39 0.40540424809064035 1104 40-41 0.6188668048936989 1104 42-43 0.5467690630726807 1104 44-45 0.5914045571245481 1104 46-47 0.5108575020101185 1104 48-49 0.6178664756506365 1104 50-51 0.5270126392368368 1104 52-53 0.49429259687922666 1104 54-55 0.6719501800896737 1104 56-57 0.7085151668915373 1104 58-59 0.49306492051575646 1104 60-61 0.43629082604733327 1104 62-63 0.6915579386439532 1104 64-65 0.711020490674322 1104 66-67 0.6189189870284437 1104 68-69 0.5206340730034427 1104 70-71 0.44471301354736426 1104 72-73 0.452908050128606 1104 74-75 0.4890011321947014 1104 76-77 0.46007060588825155 1104 78-79 0.39427276635370134 1104 80-81 0.4870346345065464 1104 82-83 0.46725996859443697 1104 84-85 0.4702849546804799 1104 86-87 0.48608452150574877 1104 88-89 0.6592921129254812 1104 90-91 0.7275771001565943 1104 92-93 0.7492524911495586 1104 94-95 0.6344606929255043 1104 96-97 0.864692551013281 1104 98-99 1.1280507891656875 1104 100-101 1.3956482668724455 1105 1 0.2550449564051718 1105 2 0.08978164954620382 1105 3 0.1325206018342584 1105 4 0.12396602915064392 1105 5 0.06955565762920202 1105 6 0.054238761984045425 1105 7 0.16117149506505513 1105 8 0.19011016923673196 1105 9 0.20598891198421398 1105 10-11 0.2594701372288881 1105 12-13 0.24448445168912514 1105 14-15 0.3150899992829821 1105 16-17 0.19638258444153678 1105 18-19 0.2020027421520254 1105 20-21 0.1345173529246395 1105 22-23 0.20309676330720805 1105 24-25 0.23224721661269854 1105 26-27 0.23623810540317436 1105 28-29 0.2942769252455335 1105 30-31 0.34363702261368445 1105 32-33 0.3029021308943669 1105 34-35 0.21133033828783 1105 36-37 0.2716081939344761 1105 38-39 0.19680366681082972 1105 40-41 0.07659732953598564 1105 42-43 0.2770713782555063 1105 44-45 0.3744524257652486 1105 46-47 0.45263636727244005 1105 48-49 0.5269377942118467 1105 50-51 0.3866813842686341 1105 52-53 0.4799531195342368 1105 54-55 0.44318925004197496 1105 56-57 0.5045629610091922 1105 58-59 0.5052568918989024 1105 60-61 0.6017233081618016 1105 62-63 0.5398184463943494 1105 64-65 0.6110968768030958 1105 66-67 0.5787063479187466 1105 68-69 0.49553036175542076 1105 70-71 0.27240950599568947 1105 72-73 0.31266498077248883 1105 74-75 0.16477989809295224 1105 76-77 0.2841881287419845 1105 78-79 0.2185834281184036 1105 80-81 0.3954327614938258 1105 82-83 0.4902223841349773 1105 84-85 0.4571303938696687 1105 86-87 0.4878712116885744 1105 88-89 0.694140955334067 1105 90-91 0.7259618129944343 1105 92-93 0.7392154780335005 1105 94-95 0.6253558389906893 1105 96-97 0.820731402364629 1105 98-99 0.6607751035536076 1105 100-101 0.7922183433827819 1106 1 0.4262145953904266 1106 2 0.28600075188792573 1106 3 0.36514202421066244 1106 4 0.32463480083320917 1106 5 0.06417973612009575 1106 6 0.1778056798071148 1106 7 0.2982303903382544 1106 8 0.35327419785772207 1106 9 0.4663829926433607 1106 10-11 0.3598241728967153 1106 12-13 0.5106244126605048 1106 14-15 0.6953646225483752 1106 16-17 0.5058436371301767 1106 18-19 0.5262363989169856 1106 20-21 0.4766723290738142 1106 22-23 0.4244486713731206 1106 24-25 0.2684740433689896 1106 26-27 0.479505329492504 1106 28-29 0.5446533210607996 1106 30-31 0.3676958636804599 1106 32-33 0.43573258677034943 1106 34-35 0.3751590731100336 1106 36-37 0.3094547762198232 1106 38-39 0.4934716660519385 1106 40-41 0.36301320117518543 1106 42-43 0.32039789061457924 1106 44-45 0.31812009976784594 1106 46-47 0.36808451883358373 1106 48-49 0.15061956188313275 1106 50-51 0.22580317253836313 1106 52-53 0.4121442784674514 1106 54-55 0.4185388814383302 1106 56-57 0.3778432623968726 1106 58-59 0.2832328242492963 1106 60-61 0.41225764792763187 1106 62-63 0.4661888524004212 1106 64-65 0.3445154723148107 1106 66-67 0.47646762285369704 1106 68-69 0.3300418941708614 1106 70-71 0.5831105304969881 1106 72-73 0.5319180840899165 1106 74-75 0.29255052363501477 1106 76-77 0.3310634675321005 1106 78-79 0.564506427901577 1106 80-81 0.2985852034929586 1106 82-83 0.5945307779988092 1106 84-85 0.4801593129936954 1106 86-87 0.4709015537310748 1106 88-89 0.7597933973332012 1106 90-91 0.7557878781505494 1106 92-93 0.8076761512772137 1106 94-95 0.9610077389256411 1106 96-97 1.3218548303785091 1106 98-99 1.3725060499976642 1106 100-101 1.3387406628754057 1107 1 0.18065837357612935 1107 2 0.161874123071172 1107 3 0.1459283174167041 1107 4 0.16676931016426266 1107 5 0.18080045717534432 1107 6 0.21070741743034205 1107 7 0.1460535393161848 1107 8 0.20976315713385674 1107 9 0.20133214193882765 1107 10-11 0.15269595511845324 1107 12-13 0.20370816575871942 1107 14-15 0.1819424245855501 1107 16-17 0.3979415386775429 1107 18-19 0.2974180693078452 1107 20-21 0.2253621902922589 1107 22-23 0.3023308782845149 1107 24-25 0.2767406451300971 1107 26-27 0.2656318979523391 1107 28-29 0.11948671628184826 1107 30-31 0.3011812264714777 1107 32-33 0.2670077710229606 1107 34-35 0.40049093775832745 1107 36-37 0.3714255870887868 1107 38-39 0.3187738114780032 1107 40-41 0.3883030784013428 1107 42-43 0.3563676622948435 1107 44-45 0.5015710197516299 1107 46-47 0.4590601097081404 1107 48-49 0.4457829388790131 1107 50-51 0.5921365421733213 1107 52-53 0.5195061168867312 1107 54-55 0.4573959444443929 1107 56-57 0.6106687666067714 1107 58-59 0.6058336695085913 1107 60-61 0.5733843804008387 1107 62-63 0.4590418058875372 1107 64-65 0.4597571689740576 1107 66-67 0.2600887231078488 1107 68-69 0.4779475090700984 1107 70-71 0.3355772915478852 1107 72-73 0.47722684253496794 1107 74-75 0.4707710573214001 1107 76-77 0.4409091243925154 1107 78-79 0.4848218452893569 1107 80-81 0.6730123047994212 1107 82-83 0.6337460462000308 1107 84-85 0.5974469124021944 1107 86-87 0.691674043572089 1107 88-89 0.920150907111676 1107 90-91 1.032510248130592 1107 92-93 1.226414088094014 1107 94-95 1.079348960208538 1107 96-97 1.183139212500791 1107 98-99 0.9933642204219844 1107 100-101 0.7972787395628096 1108 1 -0.0033950674567932992 1108 2 -0.18853608173132486 1108 3 -0.03471508098512999 1108 4 0.015617782087502974 1108 5 0.10122094764388834 1108 6 0.03937665662427747 1108 7 0.02399844991087008 1108 8 0.043345301395326885 1108 9 0.09014364766703409 1108 10-11 0.172225391450624 1108 12-13 0.0990870581502108 1108 14-15 0.04948392824773151 1108 16-17 0.05673844897898306 1108 18-19 0.026008111094753872 1108 20-21 0.03670852399659452 1108 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-1.439694551056661 2316 92-93 -1.6986209726964248 2316 94-95 -1.353769959366975 2316 96-97 -1.1470947837667538 2316 98-99 -1.1364363471763212 2316 100-101 -1.2460144349018911 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 7.0 5 25.0 6 46.0 7 151.0 8 326.0 9 642.0 10 1161.0 11 1892.0 12 2792.0 13 3873.0 14 5008.0 15 7099.0 16 9155.0 17 12282.0 18 11290.0 19 7019.0 20 6224.0 21 6217.0 22 6683.0 23 7365.0 24 8816.0 25 10356.0 26 12759.0 27 15919.0 28 19867.0 29 25149.0 30 31078.0 31 39266.0 32 49941.0 33 64663.0 34 86873.0 35 122688.0 36 182865.0 37 274330.0 38 204150.0 39 16177.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.420375867057324 20.00958567858151 15.499212889787026 22.07082556457414 2 15.442872646056713 21.367270570017677 39.60214301832601 23.587713765599595 3 19.387481720454115 25.20264979659271 30.519226813083772 24.890641669869407 4 12.987986371743935 17.700068332643628 37.562143237021786 31.74980205859066 5 13.44438446517406 37.773225732116984 34.26571419230913 14.51667561039982 6 30.7998367029301 37.16969818746631 17.480042355177506 14.550422754426084 7 27.558604962684186 33.48783249346176 21.032005485743447 17.921557058110608 8 26.16364738151432 34.77371308286024 19.864211902787524 19.198427632837916 9 25.97124768769535 17.022947630286406 20.826210371882375 36.17959431013587 10-11 24.48925892909825 27.2006796591817 27.480588570180153 20.8294728415399 12-13 25.75782341998691 24.617569973631564 28.09127449565483 21.5333321107267 14-15 22.613790351227255 25.781768586850397 26.922333243897604 24.68210781802474 16-17 21.529828729986605 29.084247623907633 27.390723671620847 21.995199974484912 18-19 21.293933788352362 27.43158767621356 30.267031319767813 21.007447215666264 20-21 22.239821836793762 26.285691048223832 30.415522780315612 21.058964334666793 22-23 21.878667793582956 26.426373030554316 30.293264017350257 21.40169515851247 24-25 21.872558025305636 26.496043402960513 30.427084429730133 21.204314142003717 26-27 21.685424615372348 26.67719184936231 30.333137979563602 21.30424555570174 28-29 21.28967439190186 26.831151848116004 30.554973028789227 21.324200731192914 30-31 21.651502863299292 26.457786571162217 30.384107153822292 21.506603411716203 32-33 21.45622815753446 26.649143524184886 30.333787558276477 21.560840760004176 34-35 22.020497126686532 26.607393031168296 30.128966205176987 21.24314363696819 36-37 21.90863564255303 26.43180166341004 30.113534652648234 21.5460280413887 38-39 21.599279180147153 26.809196034693443 30.256333586497387 21.335191198662017 40-41 21.871807977494672 26.591635615256482 30.19265562716938 21.343900780079466 42-43 21.677793911152683 26.759926610420457 29.81124748729995 21.751031991126908 44-45 21.943777879927488 26.804837218499696 29.544420705489493 21.706964196083327 46-47 22.18918103254094 27.022142735547384 29.16158523822946 21.62709099368222 48-49 22.049977056274123 27.28086748413574 28.870263695371108 21.79889176421903 50-51 21.914876058427975 27.346083173631424 28.862477220800066 21.876563547140535 52-53 22.248845126327453 27.59194237651812 28.457753770208544 21.701458726945884 54-55 21.93531385030264 27.390307283929648 28.60243502001807 22.07194384574964 56-57 22.132316444770474 27.585321561771792 28.4877636491575 21.794598344300233 58-59 22.42328921952649 27.349204735506532 28.349449922955326 21.878056122011653 60-61 22.10872284898931 27.358881722419216 28.90499727702503 21.62739815156645 62-63 22.033951011035274 27.360265037953692 28.901814760477134 21.703969190533904 64-65 21.867118710017962 27.44066333002965 28.673483463394135 22.018734496558245 66-67 21.934140726973215 27.56819510125909 28.432519321712796 22.065144850054896 68-69 21.907451999744847 27.4258467819098 28.615368055112583 22.051333163232762 70-71 22.08331971196589 27.325247158051187 28.550572217035032 22.040860912947903 72-73 22.067678765069846 27.36696274797474 28.52299547107227 22.04236301588314 74-75 21.93421068368742 27.811634128158 28.128500863130952 22.12565432502362 76-77 22.067283100844918 27.516159370281905 28.26371837146877 22.152839157404404 78-79 22.102504751201305 27.605565972029805 28.153503968603598 22.13842530816529 80-81 22.14958908631759 27.772167271152792 28.022626665991545 22.05561697653807 82-83 22.177733958410496 27.67162478466294 28.22307902994765 21.92756222697892 84-85 22.048558506549636 27.72929298406208 28.0252384946847 22.196910014703583 86-87 22.058040824169975 27.491588820816272 28.22165405704093 22.228716297972824 88-89 22.038245915351233 27.57443904103745 28.010273117904045 22.37704192570727 90-91 21.97147408859171 27.499366108576435 28.225151854869964 22.3040079479619 92-93 22.1050432580545 27.494110597575506 28.18202150523917 22.218824639130826 94-95 21.933250010465194 27.303787602434305 28.151288813406612 22.611673573693896 96-97 21.785392921116767 27.396376078080692 28.291691866568165 22.526539134234376 98-99 21.74361129295518 27.50152097833792 27.90219810274398 22.85266962596292 100-101 21.63161226842808 27.536905591534 28.037046723427732 22.794435416610188 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 460.0 1 336.5 2 260.0 3 320.0 4 267.0 5 237.0 6 455.5 7 1043.5 8 1748.5 9 2089.0 10 1885.5 11 1528.5 12 1493.5 13 1619.5 14 1745.0 15 1843.5 16 2007.5 17 2136.5 18 2202.0 19 2438.5 20 2749.0 21 3090.5 22 3621.0 23 4366.0 24 5117.5 25 6225.5 26 7977.5 27 9909.5 28 11489.0 29 13351.0 30 15737.5 31 18093.5 32 20702.0 33 23223.0 34 25927.0 35 28539.5 36 31635.0 37 35240.0 38 37150.0 39 37963.5 40 39183.0 41 40829.5 42 42293.0 43 43736.5 44 45269.0 45 46343.5 46 46166.0 47 47469.5 48 52152.0 49 51954.5 50 46894.0 51 43613.0 52 41277.0 53 39641.5 54 37843.5 55 34614.0 56 31082.5 57 27942.0 58 25270.0 59 22822.0 60 19640.5 61 15511.0 62 11885.0 63 9143.0 64 6727.0 65 4963.5 66 3587.0 67 2622.5 68 1798.0 69 1111.5 70 774.5 71 582.0 72 427.0 73 324.0 74 240.5 75 168.0 76 115.5 77 55.5 78 32.5 79 22.5 80 12.0 81 8.0 82 5.0 83 4.5 84 3.0 85 1.0 86 1.0 87 1.5 88 1.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016425336480193915 2 0.003269120367417235 3 0.0020731007208011736 4 7.973464310773745E-5 5 0.0018338967914779613 6 3.189385724309498E-4 7 0.0 8 0.0 9 0.0 10-11 1.594692862154749E-4 12-13 7.176117879696371E-4 14-15 3.9867321553868726E-5 16-17 0.0 18-19 0.0 20-21 0.002312304650124386 22-23 0.0 24-25 0.004146201441602347 26-27 0.001993366077693436 28-29 0.0035880589398481853 30-31 0.014910378261146903 32-33 7.176117879696371E-4 34-35 0.002631243222555336 36-37 0.003946864833833004 38-39 0.002591375901001467 40-41 0.004544874657141035 42-43 0.002471773936339861 44-45 7.574791095235057E-4 46-47 1.1960196466160617E-4 48-49 3.5880589398481854E-4 50-51 3.9867321553868726E-5 52-53 0.0014750908974931428 54-55 0.0034285896536327106 56-57 5.980098233080308E-4 58-59 0.0013953562543854055 60-61 0.0013554889328315368 62-63 0.0 64-65 2.3920392932321233E-4 66-67 7.973464310773745E-5 68-69 0.0 70-71 2.3920392932321233E-4 72-73 0.0 74-75 3.986732155386872E-4 76-77 9.169483957389807E-4 78-79 2.790712508770811E-4 80-81 0.005142884480449065 82-83 0.0017142948268163553 84-85 0.003508324296740448 86-87 0.002033233399247305 88-89 0.0010365503604005868 90-91 1.594692862154749E-4 92-93 3.9867321553868726E-5 94-95 1.993366077693436E-4 96-97 0.0017142948268163553 98-99 0.002631243222555336 100-101 0.0011162850035083243 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1254160.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.58991063648992 #Duplication Level Percentage of deduplicated Percentage of total 1 82.66590724838217 53.39383561857368 2 10.750797576281629 13.887861094460455 3 2.9605995532786764 5.736745817301049 4 1.1596794131917172 2.996143586601203 5 0.6227058716759538 2.0110258302183706 6 0.38017476805102685 1.473327257679846 7 0.2408953600161661 1.0891586843332464 8 0.1819010639300862 0.9399178771141363 9 0.13659313351958086 0.7940284458829076 >10 0.8107338262944779 9.646209968629043 >50 0.0595126466853844 2.6591137435783363 >100 0.028647352501533403 3.2716453975952318 >500 0.001111311714990551 0.4821191386215198 >1k 4.939163177735781E-4 0.6734514485558499 >5k 2.4695815888678906E-4 0.9454160908551686 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6440 0.5134911016138292 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5414 0.43168335778529054 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3467 0.27644000765452575 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2441 0.1946322638259871 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1434 0.11433947821649551 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 7.973464310773745E-5 0.0 0.0 0.0 0.0 8 7.973464310773745E-5 0.0 0.0 0.0 0.0 9 7.973464310773745E-5 0.0 0.0 0.0 0.0 10-11 7.973464310773745E-5 0.0 0.0 0.0 0.0 12-13 1.594692862154749E-4 0.0 0.0 0.0 0.0 14-15 1.594692862154749E-4 0.0 0.0 0.0 0.0 16-17 1.594692862154749E-4 0.0 0.0 0.0 0.0 18-19 1.594692862154749E-4 0.0 0.0 0.0 0.0 20-21 1.594692862154749E-4 0.0 0.0 3.9867321553868726E-5 0.0 22-23 1.594692862154749E-4 0.0 0.0 1.9933660776934363E-4 0.0 24-25 1.594692862154749E-4 0.0 0.0 5.98009823308031E-4 0.0 26-27 1.594692862154749E-4 0.0 0.0 8.77081074185112E-4 0.0 28-29 1.594692862154749E-4 0.0 0.0 0.001594692862154749 0.0 30-31 1.594692862154749E-4 0.0 0.0 0.002990049116540154 3.9867321553868726E-5 32-33 1.594692862154749E-4 0.0 0.0 0.005103017158895197 7.973464310773745E-5 34-35 1.594692862154749E-4 0.0 0.0 0.0071761178796963705 7.973464310773745E-5 36-37 1.594692862154749E-4 0.0 0.0 0.009568157172928495 7.973464310773745E-5 38-39 1.594692862154749E-4 0.0 0.0 0.011402053964406456 7.973464310773745E-5 40-41 1.594692862154749E-4 0.0 0.0 0.01439210308094661 7.973464310773745E-5 42-43 1.594692862154749E-4 0.0 0.0 0.019216048988964726 7.973464310773745E-5 44-45 1.594692862154749E-4 0.0 0.0 0.026711105441092044 7.973464310773745E-5 46-47 1.594692862154749E-4 0.0 0.0 0.0419404222746699 7.973464310773745E-5 48-49 1.594692862154749E-4 0.0 0.0 0.07267812719270268 7.973464310773745E-5 50-51 1.594692862154749E-4 0.0 0.0 0.09332939975760668 7.973464310773745E-5 52-53 1.594692862154749E-4 0.0 0.0 0.11158863302927857 1.594692862154749E-4 54-55 1.594692862154749E-4 0.0 0.0 0.1339940677425528 1.594692862154749E-4 56-57 1.594692862154749E-4 0.0 0.0 0.15364865726861007 1.594692862154749E-4 58-59 1.594692862154749E-4 0.0 0.0 0.1786056005613319 1.594692862154749E-4 60-61 1.594692862154749E-4 0.0 0.0 0.2172370351470307 1.594692862154749E-4 62-63 1.594692862154749E-4 0.0 0.0 0.24649964916757033 1.594692862154749E-4 64-65 1.594692862154749E-4 0.0 0.0 0.28293838106780633 1.594692862154749E-4 66-67 1.594692862154749E-4 0.0 0.0 0.34572941251514955 1.594692862154749E-4 68-69 1.594692862154749E-4 0.0 0.0 0.41788926452765196 1.594692862154749E-4 70-71 1.594692862154749E-4 0.0 0.0 0.4796437456145946 1.594692862154749E-4 72-73 1.594692862154749E-4 0.0 0.0 0.5471789883268483 2.3920392932321236E-4 74-75 1.594692862154749E-4 0.0 0.0 0.6059035529756969 2.3920392932321236E-4 76-77 1.594692862154749E-4 0.0 0.0 0.6541031447343242 2.3920392932321236E-4 78-79 1.594692862154749E-4 0.0 0.0 0.7091599158002169 2.3920392932321236E-4 80-81 1.594692862154749E-4 0.0 0.0 0.8109013204056899 2.3920392932321236E-4 82-83 1.594692862154749E-4 0.0 0.0 0.9266760221981247 3.189385724309498E-4 84-85 1.594692862154749E-4 0.0 0.0 1.048630158831409 3.189385724309498E-4 86-87 1.594692862154749E-4 0.0 0.0 1.2465315430248134 3.189385724309498E-4 88-89 1.594692862154749E-4 0.0 0.0 1.4874497671748421 3.189385724309498E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8535 0.0 28.892265 1 GGTATCA 5995 0.0 27.739372 1 TCAACGC 10330 0.0 23.679296 4 ATCAACG 10335 0.0 23.669727 3 GTACATG 5075 0.0 23.499329 1 CAACGCA 10445 0.0 23.418587 5 GAGTACT 5805 0.0 23.35962 12-13 TATCAAC 10500 0.0 23.343012 2 AACGCAG 10665 0.0 23.024572 6 AGAGTAC 8595 0.0 22.187153 10-11 TACATGG 5075 0.0 22.182417 2 AGTACTT 6165 0.0 21.648865 12-13 ATTCGCG 55 0.0028791914 21.589764 80-81 GTACTTT 6265 0.0 21.568655 14-15 ACATGGG 5090 0.0 21.463799 3 ACGCAGA 11705 0.0 20.897661 7 CGCAGAG 11725 0.0 20.821508 8 CATGGGA 2925 0.0 19.972898 4 GCAGAGT 12185 0.0 19.567715 9 TACTTTT 6965 0.0 18.9236 14-15 >>END_MODULE