FastQCFastQC Report
Thu 26 May 2016
SRR936874_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936874_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197554
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57640.48131441254423596No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43830.36599602189128844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28990.24207676647566623No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20830.17393787670535107No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13200.110224674628451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77550.027.9482381
GGTATCA53600.027.2235621
GTACATG52350.024.0602741
TCAACGC93000.023.2541164
ATCAACG92800.023.2530143
CAACGCA93100.023.1790545
TATCAAC93350.023.165962
TACATGG53250.022.8493352
AACGCAG95200.022.7638026
GTACCTG9300.021.9764181
GAGTACT46050.021.92946812-13
TACCTGG9150.021.8163152
ACATGGG54100.021.2612843
AGAGTAC75650.021.20091210-11
ACGCAGA104850.020.7140357
CGCAGAG104950.020.6037318
AGTACTT48950.020.2419412-13
GTACTTT48850.020.08965114-15
CCTATAC4050.019.9510273
TACCGGG1200.00474716719.8035012
Produced by FastQC (version 0.11.5)