##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936874_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1197554 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.702612157781612 33.0 31.0 34.0 30.0 34.0 2 31.818692935767405 33.0 31.0 34.0 30.0 34.0 3 31.826507197170233 33.0 31.0 34.0 30.0 34.0 4 35.416088961332854 37.0 35.0 37.0 33.0 37.0 5 35.42429401930936 37.0 35.0 37.0 33.0 37.0 6 35.49795583330689 37.0 35.0 37.0 33.0 37.0 7 35.43123566870471 37.0 35.0 37.0 33.0 37.0 8 35.38212974112232 37.0 35.0 37.0 33.0 37.0 9 37.00583105229493 39.0 37.0 39.0 33.0 39.0 10-11 36.8169685876378 39.0 37.0 39.0 33.0 39.0 12-13 36.74473844185732 39.0 37.0 39.0 32.0 39.0 14-15 37.88355180643211 40.0 38.0 41.0 33.0 41.0 16-17 37.82541872850828 40.0 38.0 41.0 32.5 41.0 18-19 37.794573355356 40.0 37.5 41.0 32.0 41.0 20-21 37.727619380837936 40.0 37.5 41.0 32.0 41.0 22-23 37.626712866392666 40.0 37.0 41.0 32.0 41.0 24-25 37.46201089888223 40.0 37.0 41.0 32.0 41.0 26-27 37.554043491984494 40.0 37.0 41.0 32.0 41.0 28-29 37.50620681823116 40.0 37.0 41.0 32.0 41.0 30-31 37.35718973841681 40.0 37.0 41.0 31.5 41.0 32-33 37.15111051359688 40.0 36.0 41.0 31.0 41.0 34-35 36.97955248782101 39.5 36.0 41.0 31.0 41.0 36-37 36.80364893775145 39.0 36.0 41.0 30.0 41.0 38-39 36.64366492032927 39.0 35.5 41.0 30.0 41.0 40-41 36.476084168229576 39.0 35.0 41.0 30.0 41.0 42-43 36.261670037426285 39.0 35.0 41.0 30.0 41.0 44-45 35.99931318337211 39.0 35.0 40.5 29.0 41.0 46-47 35.64079532112957 39.0 35.0 40.0 27.5 41.0 48-49 35.307084690961744 39.0 35.0 40.0 26.0 41.0 50-51 34.649077202364154 38.0 34.0 39.5 25.0 40.5 52-53 34.88706396538277 38.5 34.5 40.0 26.0 40.5 54-55 35.26990014646521 39.0 35.0 40.5 26.0 41.0 56-57 35.16292167200811 39.0 35.0 41.0 26.0 41.0 58-59 34.93017225110517 38.5 34.5 40.0 26.0 41.0 60-61 34.640920993959355 38.0 34.0 40.0 25.5 41.0 62-63 34.327183993373154 38.0 34.0 40.0 24.5 41.0 64-65 34.01458973875082 37.0 34.0 40.0 23.0 41.0 66-67 33.636592587891656 37.0 33.5 39.5 22.5 41.0 68-69 33.25696503038694 36.0 33.0 39.0 22.0 41.0 70-71 32.83689754282479 36.0 32.5 39.0 21.5 40.5 72-73 32.44226398141545 35.0 32.0 38.5 20.0 40.0 74-75 32.010771539320984 35.0 32.0 37.5 18.5 39.5 76-77 31.58251611200831 35.0 31.5 37.0 18.0 39.0 78-79 31.12581311573424 35.0 31.0 36.5 14.0 39.0 80-81 30.682707418621625 35.0 31.0 36.0 9.5 38.5 82-83 30.27096231151163 34.0 30.0 35.5 7.0 37.0 84-85 29.911561399318945 34.0 30.0 35.0 4.5 37.0 86-87 29.583151156440543 34.0 30.0 35.0 2.0 36.0 88-89 29.227814361607077 34.0 29.5 35.0 2.0 36.0 90-91 28.868200932901566 34.0 29.0 35.0 2.0 36.0 92-93 28.496249855956393 34.0 29.0 35.0 2.0 35.0 94-95 28.063072729914477 34.0 29.0 35.0 2.0 35.0 96-97 27.586645779647515 34.0 27.0 35.0 2.0 35.0 98-99 26.99312056074298 34.0 26.5 35.0 2.0 35.0 100-101 24.778158646708206 31.5 21.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4725892975609547 1101 2 0.3749787450951949 1101 3 0.41654314983437857 1101 4 0.3962661344955336 1101 5 0.34301556899148977 1101 6 0.24547934469989485 1101 7 0.2039373671015383 1101 8 0.2967352434284294 1101 9 0.23453967309201573 1101 10-11 0.44181886003539006 1101 12-13 0.47433706848603663 1101 14-15 0.6280831525205954 1101 16-17 0.6902008754291487 1101 18-19 0.6117560538680635 1101 20-21 0.5727365237825737 1101 22-23 0.6710901497304462 1101 24-25 0.5885984678558245 1101 26-27 0.5824544665577207 1101 28-29 0.6462534899468935 1101 30-31 0.6929107400147743 1101 32-33 0.7186978901613159 1101 34-35 0.671734377154344 1101 36-37 0.7170557864093112 1101 38-39 0.5466658567990947 1101 40-41 0.6960921352075999 1101 42-43 0.7169354179827963 1101 44-45 0.7463617580098116 1101 46-47 0.6768342677929553 1101 48-49 0.5689255932377577 1101 50-51 0.4325868452272914 1101 52-53 0.4589928161048533 1101 54-55 0.3382166072687838 1101 56-57 0.41412688771161754 1101 58-59 0.48816561764191846 1101 60-61 0.44069712616602885 1101 62-63 0.6117386795562822 1101 64-65 0.5634337555634303 1101 66-67 0.5120171801809832 1101 68-69 0.6028142888675916 1101 70-71 0.28992820812692344 1101 72-73 0.3330977703603466 1101 74-75 0.32098407103116955 1101 76-77 0.4054515043261482 1101 78-79 0.48285178740126256 1101 80-81 0.344792446969425 1101 82-83 0.39068441690513467 1101 84-85 0.5811398451415108 1101 86-87 0.5070937296485134 1101 88-89 0.4665683215697918 1101 90-91 0.6273791982499866 1101 92-93 0.5492082825678999 1101 94-95 0.6893289559751956 1101 96-97 0.575950690120326 1101 98-99 0.6974700552305713 1101 100-101 0.9847349138903461 1102 1 0.4801240132548692 1102 2 0.47465307562620396 1102 3 0.3615775297865689 1102 4 0.3106419234885749 1102 5 0.32160397650135764 1102 6 0.25279112789132085 1102 7 0.2539601975385395 1102 8 0.33145630920694913 1102 9 0.4330617380378925 1102 10-11 0.6756126071815842 1102 12-13 0.5881739920175022 1102 14-15 0.6798400889445375 1102 16-17 0.7923348498053358 1102 18-19 0.7268540233021312 1102 20-21 0.6839912577408356 1102 22-23 0.5567637147703266 1102 24-25 0.7458484745706642 1102 26-27 0.7564432124952418 1102 28-29 0.7457475271739398 1102 30-31 0.9140564250621779 1102 32-33 0.9066595350271527 1102 34-35 0.6674291610709773 1102 36-37 0.4940615074482295 1102 38-39 0.7268335933627128 1102 40-41 0.8354189059094281 1102 42-43 0.7987066570040469 1102 44-45 0.6454310819602824 1102 46-47 0.5297521990867651 1102 48-49 0.5620348301639808 1102 50-51 0.7807510094989993 1102 52-53 0.700481427745693 1102 54-55 0.7069164138815509 1102 56-57 0.8473508811579364 1102 58-59 0.8917113859532719 1102 60-61 0.7424799147024572 1102 62-63 0.8064212021852768 1102 64-65 0.6129489445188696 1102 66-67 0.4785880485433651 1102 68-69 0.4468008189097574 1102 70-71 0.5361571571210462 1102 72-73 0.8179151940126772 1102 74-75 0.5490903534302376 1102 76-77 0.574301051746307 1102 78-79 0.7152401197049905 1102 80-81 0.7277337458869972 1102 82-83 0.505385539625447 1102 84-85 0.7563231601209672 1102 86-87 0.6774037535533246 1102 88-89 0.7510456388591429 1102 90-91 0.9163087864963408 1102 92-93 1.246346084399704 1102 94-95 1.028427422307015 1102 96-97 0.9350066515503137 1102 98-99 0.8274333250863357 1102 100-101 0.8698900937136145 1103 1 0.20229346372025248 1103 2 0.3226863537085727 1103 3 0.35328032208543547 1103 4 0.2546397559506488 1103 5 0.16396978040292964 1103 6 0.13521813286390483 1103 7 -0.029247758600689622 1103 8 0.15714800881168856 1103 9 0.10766648112090138 1103 10-11 0.23767113070574197 1103 12-13 0.2503297359930201 1103 14-15 0.3385915233446397 1103 16-17 0.3176631680509061 1103 18-19 0.3618964177940427 1103 20-21 0.4957055252405169 1103 22-23 0.3697737236954879 1103 24-25 0.24237085121225732 1103 26-27 0.5057897635598891 1103 28-29 0.38096204967808234 1103 30-31 0.43854004102546185 1103 32-33 0.5245990989695244 1103 34-35 0.5622956024836938 1103 36-37 0.5125493309851379 1103 38-39 0.514794195173252 1103 40-41 0.5461067804472393 1103 42-43 0.5136084802859529 1103 44-45 0.5956095850840839 1103 46-47 0.6814368223176714 1103 48-49 0.6416153478230342 1103 50-51 0.7305661312636289 1103 52-53 0.643244082342143 1103 54-55 0.777793310477243 1103 56-57 0.8098126304885582 1103 58-59 0.6141693102169441 1103 60-61 0.8108497986754273 1103 62-63 0.7348765126532086 1103 64-65 0.6858929710393369 1103 66-67 0.6123123887193174 1103 68-69 0.7622907462334894 1103 70-71 0.7684470971949935 1103 72-73 0.797567431676832 1103 74-75 0.7448923424650502 1103 76-77 0.9304418779607637 1103 78-79 0.8745987834816056 1103 80-81 0.7678029158474722 1103 82-83 0.7941541868267734 1103 84-85 0.741909032873739 1103 86-87 0.7744759835673491 1103 88-89 0.7633782921805796 1103 90-91 0.7488234492529351 1103 92-93 0.9553875863759878 1103 94-95 1.024949798948608 1103 96-97 1.0193414086585655 1103 98-99 1.264904637084296 1103 100-101 1.40166843108452 1104 1 0.3026982068032531 1104 2 0.32433694659394874 1104 3 0.26333882010915133 1104 4 0.2969322460692254 1104 5 0.2405136539207149 1104 6 0.17879757950422004 1104 7 0.2052376168933776 1104 8 0.31465630920694565 1104 9 0.19046173803789657 1104 10-11 0.5089738975041556 1104 12-13 0.3356546371787914 1104 14-15 0.3010626695897045 1104 16-17 0.5223477530311484 1104 18-19 0.5403991845924594 1104 20-21 0.4643331932247108 1104 22-23 0.4150701663832308 1104 24-25 0.48548073263518177 1104 26-27 0.320178696366213 1104 28-29 0.3608023658836217 1104 30-31 0.508927392804118 1104 32-33 0.5188014705110291 1104 34-35 0.4980710965548454 1104 36-37 0.4779453784159742 1104 38-39 0.4764400449756181 1104 40-41 0.4834253575223286 1104 42-43 0.462738915068563 1104 44-45 0.7512633400247921 1104 46-47 0.5777941345706452 1104 48-49 0.6772090237123649 1104 50-51 0.745796170789319 1104 52-53 0.6722717503263382 1104 54-55 0.7580873816234899 1104 56-57 0.4851153972869682 1104 58-59 0.6236823536952087 1104 60-61 0.699196043734716 1104 62-63 0.7054599118626896 1104 64-65 0.7343166864543562 1104 66-67 0.7314396614465934 1104 68-69 0.7769911414904058 1104 70-71 0.7112281248629841 1104 72-73 1.0155184198191307 1104 74-75 0.771470998591532 1104 76-77 0.6166268581979217 1104 78-79 0.5163885068017642 1104 80-81 0.7188337458869931 1104 82-83 0.6946790880125455 1104 84-85 0.6521683214112848 1104 86-87 0.827258592263 1104 88-89 0.7846778969236645 1104 90-91 0.8491507219802088 1104 92-93 0.9478460843997034 1104 94-95 0.9432596803715363 1104 96-97 0.9683453612277368 1104 98-99 1.1890817121831105 1104 100-101 1.0870739646813554 1105 1 0.2558664607715073 1105 2 0.34227980373680467 1105 3 0.4595165978869282 1105 4 0.3874084365454138 1105 5 0.3726152412222987 1105 6 0.40677218267882864 1105 7 0.33109793435369994 1105 8 0.4814563092069477 1105 9 0.5152045951807551 1105 10-11 0.5318850086152693 1105 12-13 0.577222891147045 1105 14-15 0.45647219339922174 1105 16-17 0.37450330858670355 1105 18-19 0.29023727983055636 1105 20-21 0.46284430433581747 1105 22-23 0.5530066743197466 1105 24-25 0.5149600977145425 1105 26-27 0.6440136170011357 1105 28-29 0.45339601667726726 1105 30-31 0.04594326581999297 1105 32-33 0.10733797844753212 1105 34-35 0.20044887433261493 1105 36-37 0.24621204508263617 1105 38-39 0.10581464815022912 1105 40-41 0.3667809130778892 1105 42-43 0.34162780395745074 1105 44-45 0.4189077844692406 1105 46-47 0.4754703250468282 1105 48-49 0.3516661665695082 1105 50-51 0.28996759936075023 1105 52-53 0.2714939725485621 1105 54-55 0.4088429371790383 1105 56-57 0.6377566671282437 1105 58-59 0.6662569568698089 1105 60-61 0.8093769961156738 1105 62-63 0.6513392769420534 1105 64-65 0.6382817658194355 1105 66-67 0.714801566208493 1105 68-69 0.8309086018078702 1105 70-71 0.779551934386788 1105 72-73 0.6294295309302385 1105 74-75 0.39389322081375866 1105 76-77 0.5078840010550643 1105 78-79 0.33259803061128324 1105 80-81 0.42237660302985347 1105 82-83 0.5607298816633381 1105 84-85 0.4754603849033501 1105 86-87 0.44933795734236526 1105 88-89 0.4165858334316006 1105 90-91 0.5921856426151315 1105 92-93 0.43265560820922744 1105 94-95 0.5523009502128033 1105 96-97 0.5999644088467839 1105 98-99 0.5969737756751741 1105 100-101 0.5452549170623087 1106 1 0.20229036366600184 1106 2 0.20788204463316262 1106 3 0.15899372206993334 1106 4 0.2620302852849079 1106 5 0.2104116931364004 1106 6 0.20791130499441834 1106 7 0.22324546003063261 1106 8 0.37106415234420353 1106 9 0.20021860078299625 1106 10-11 0.30518566221004306 1106 12-13 0.303842872472913 1106 14-15 0.46481953233479345 1106 16-17 0.5115948118546783 1106 18-19 0.37725408655324344 1106 20-21 0.5037920167541188 1106 22-23 0.21977604873617906 1106 24-25 0.2412532816547852 1106 26-27 0.18475124538581866 1106 28-29 0.3617200129424347 1106 30-31 0.2725352359413691 1106 32-33 0.3183230391384697 1106 34-35 0.4378671749862164 1106 36-37 0.28405518233753924 1106 38-39 0.28587533909326623 1106 40-41 0.3028920241889992 1106 42-43 0.503719307225424 1106 44-45 0.3577966733581235 1106 46-47 0.25166080123730694 1106 48-49 0.3668482393986352 1106 50-51 0.5080628374559808 1106 52-53 0.44544822091457803 1106 54-55 0.39054228358427423 1106 56-57 0.28517029924775983 1106 58-59 0.4171019615383429 1106 60-61 0.4016038868719676 1106 62-63 0.39606383343132023 1106 64-65 0.5145049217484754 1106 66-67 0.6058239751720791 1106 68-69 0.6207872199217732 1106 70-71 0.5225771444708229 1106 72-73 0.5228282237406958 1106 74-75 0.5107886456503579 1106 76-77 0.6134582307469465 1106 78-79 0.5685924283703905 1106 80-81 0.5901357066713082 1106 82-83 0.576294774287053 1106 84-85 0.6438310665093248 1106 86-87 0.33435663147868766 1106 88-89 0.37390142633542567 1106 90-91 0.35880562394099513 1106 92-93 0.3822774569487244 1106 94-95 0.4657930137048645 1106 96-97 0.32234536122773605 1106 98-99 0.4725013200262502 1106 100-101 0.3676778862499823 1107 1 0.2749544038779277 1107 2 0.23594526068170651 1107 3 0.29182350063262774 1107 4 0.3536681968005553 1107 5 0.273535362927646 1107 6 0.22184238319937322 1107 7 0.23646286708583375 1107 8 0.3158289035102584 1107 9 0.4738046171756949 1107 10-11 0.5252581161338767 1107 12-13 0.4048943600425332 1107 14-15 0.2577588974572862 1107 16-17 0.3790901702751839 1107 18-19 0.47057655179799696 1107 20-21 0.5011365804456531 1107 22-23 0.3261710131884712 1107 24-25 0.5348691852910719 1107 26-27 0.4014002514085533 1107 28-29 0.5010434744286485 1107 30-31 0.42273493706893817 1107 32-33 0.5610835336365056 1107 34-35 0.4175710195725557 1107 36-37 0.4947493814952608 1107 38-39 0.28449146914806533 1107 40-41 0.5893431404322698 1107 42-43 0.5937704778091302 1107 44-45 0.8266188442588174 1107 46-47 0.6086276988508601 1107 48-49 0.6749368143205174 1107 50-51 0.4125893963474425 1107 52-53 0.4876281783478902 1107 54-55 0.7209548181130927 1107 56-57 0.8944742887419395 1107 58-59 0.9356574114319258 1107 60-61 0.9216419251819872 1107 62-63 0.8032729988526839 1107 64-65 0.9300290806037026 1107 66-67 0.8711828485135626 1107 68-69 0.8541169305589236 1107 70-71 0.9733383635080912 1107 72-73 0.960928119588182 1107 74-75 0.9181490586377237 1107 76-77 0.9642955264042321 1107 78-79 1.1066714937147744 1107 80-81 0.7625008744474293 1107 82-83 0.9160783181896015 1107 84-85 0.9214563891557042 1107 86-87 0.7853170988064946 1107 88-89 0.8428377121661548 1107 90-91 0.8159310145129268 1107 92-93 0.867602820350438 1107 94-95 0.8512433601251885 1107 96-97 0.905039741520266 1107 98-99 1.1016495335841903 1107 100-101 0.6089440185689625 1108 1 0.1527616290952487 1108 2 0.11841795130352395 1108 3 0.19429768981087747 1108 4 0.226445589868284 1108 5 0.17244081247644516 1108 6 0.11662740681976658 1108 7 0.06913212238788446 1108 8 0.05924280842202023 1108 9 0.07713552139739477 1108 10-11 0.14200623659364453 1108 12-13 0.20496578317565195 1108 14-15 0.21037067586913594 1108 16-17 0.3006981454958222 1108 18-19 0.3098735958954464 1108 20-21 0.19235328741623192 1108 22-23 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-2.8593769351983553 2316 86-87 -3.2863090593699695 2316 88-89 -3.1212829117855136 2316 90-91 -2.981093134940476 2316 92-93 -3.040648472365458 2316 94-95 -3.7082868204060695 2316 96-97 -3.8476546387722657 2316 98-99 -3.5783378834623 2316 100-101 -3.476916081974945 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2668.0 3 944.0 4 1028.0 5 1022.0 6 1135.0 7 1365.0 8 1680.0 9 2198.0 10 2699.0 11 3290.0 12 4284.0 13 5188.0 14 5708.0 15 7278.0 16 8804.0 17 11626.0 18 10296.0 19 7006.0 20 6684.0 21 6981.0 22 7605.0 23 8395.0 24 9454.0 25 11062.0 26 12687.0 27 15536.0 28 18003.0 29 22165.0 30 26759.0 31 33215.0 32 42637.0 33 55495.0 34 75287.0 35 107298.0 36 160370.0 37 237975.0 38 220986.0 39 40718.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.64575066206631 19.314709150299496 14.801630729902005 22.237909457732183 2 15.125810332152643 20.785353872886454 40.46723584842612 23.621599946534786 3 19.159524467790988 25.355341969400957 30.112668691902204 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26.69758113691076 29.82573961679756 21.488814491460722 24-25 21.90136189784576 26.779940309177913 29.94212570985684 21.37657208311949 26-27 21.656631574815545 26.938907942038526 29.901748106180026 21.502712376965903 28-29 21.353145084944792 27.152141440605877 29.999586582385152 21.495126892064178 30-31 21.630295893568512 26.759869382573765 29.957657905945435 21.65217681791229 32-33 21.45339801528082 26.947744747375463 29.86594836189266 21.732908875451063 34-35 22.013074701004737 26.94427301205 29.631242656128514 21.411409630816745 36-37 21.940722845082973 26.668498296208735 29.725092726245567 21.665686132462724 38-39 21.580007081750797 27.166865423351737 29.712036638039024 21.541090856858442 40-41 21.924652054602333 26.92407583397497 29.68947553901682 21.46179657240588 42-43 21.63390756748135 27.045651740030515 29.34869839053545 21.971742301952684 44-45 21.84678308954006 27.122220635624466 29.17210493924583 21.858891335589643 46-47 22.087307903322646 27.301297944316076 28.84400033736698 21.767393814994293 48-49 22.05230402066909 27.4782741577087 28.56838661352426 21.901035208097955 50-51 21.86187467666218 27.63809212836558 28.598533325038073 21.901499869934167 52-53 22.122985924173168 27.773818068549872 28.224598604484342 21.878597402792618 54-55 21.817345946866826 27.565088638800646 28.441371788576337 22.176193625756195 56-57 22.0101690314363 27.87943329052735 28.19022561885969 21.92017205917666 58-59 22.28965316813633 27.520399786544452 28.233713784167712 21.95623326115151 60-61 22.126135215688368 27.55036459283827 28.610504767206397 21.71299542426696 62-63 21.932274717790445 27.720279626835726 28.575145014350063 21.77230064102377 64-65 21.816957962532406 27.72275615329111 28.42539886154093 22.03488702263556 66-67 21.868957966026386 27.854387346628904 28.106821699081546 22.16983298826316 68-69 21.761993220950572 27.787378739711567 28.326169502964373 22.124458536373492 70-71 21.954238460957033 27.690821675656917 28.23240824995584 22.122531613430212 72-73 21.973103533522867 27.582784056552402 28.310694109904354 22.13341830002038 74-75 21.78576544169325 28.094863647392067 28.01894399861023 22.100426912304457 76-77 21.963196435745783 27.779958754482255 28.018344148657597 22.23850066111436 78-79 21.97847683848022 27.90455127361732 27.906263770458594 22.21070811744387 80-81 22.013416820225302 28.065269798204746 27.866214371751667 22.055099009818285 82-83 22.023061225683097 27.901050902927842 28.040129940751495 22.035757930637565 84-85 21.99846232936638 28.000472743931905 27.846329824086634 22.154735102615085 86-87 21.97252671987812 27.816487309101966 28.073287138041287 22.13769883297863 88-89 22.023374512543775 27.805154486768835 27.77554587997698 22.395925120710405 90-91 21.930038122529126 27.824142765092308 28.02116554130943 22.22465357106914 92-93 22.148662922479286 27.837533397179705 27.930421097870813 22.083382582470204 94-95 21.90544482012081 27.667701261559845 27.98004256428075 22.446811354038594 96-97 21.839521668396575 27.71382805206847 28.071908460396255 22.374741819138695 98-99 21.716326284220145 27.87274582878847 27.75957512316557 22.651352763825813 100-101 21.788201988060937 27.814093908137412 27.825499856276696 22.57220424752495 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 310.0 1 231.0 2 215.0 3 289.0 4 253.5 5 230.0 6 407.0 7 909.5 8 1623.5 9 1932.0 10 1644.0 11 1372.5 12 1361.0 13 1463.5 14 1659.0 15 1779.5 16 1827.0 17 1893.0 18 1935.5 19 2122.5 20 2399.5 21 2698.0 22 3221.0 23 3946.5 24 4837.0 25 5976.5 26 7565.5 27 9533.0 28 11084.0 29 12916.5 30 15304.0 31 17571.0 32 19914.0 33 22346.0 34 25119.0 35 27785.0 36 30892.5 37 34002.5 38 35779.0 39 36730.5 40 37689.5 41 39141.0 42 40539.0 43 41961.0 44 43268.5 45 44279.5 46 44613.0 47 45806.5 48 49496.5 49 49132.5 50 44696.0 51 41201.0 52 38627.0 53 37377.5 54 35740.0 55 32909.5 56 29905.5 57 26751.0 58 24049.5 59 21693.0 60 18392.0 61 14546.5 62 11253.5 63 8583.5 64 6459.0 65 4840.0 66 3485.5 67 2538.0 68 1756.5 69 1149.5 70 788.5 71 554.0 72 410.0 73 311.0 74 231.0 75 172.0 76 114.5 77 51.0 78 25.0 79 20.5 80 17.0 81 12.0 82 9.0 83 5.0 84 4.0 85 4.5 86 3.0 87 3.5 88 3.5 89 1.5 90 1.0 91 3.0 92 3.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.045425926513543435 2 0.042920820271987736 3 0.027305658032957175 4 0.047430011506788 5 0.06897392518416706 6 0.06847290393585592 7 0.06889042164278188 8 0.07030998184633011 9 0.07198005267403391 10-11 0.07953712316939363 12-13 0.0771155204692231 14-15 0.07056049247048567 16-17 0.05995554271456653 18-19 0.04955935181211035 20-21 0.058953500217944245 22-23 0.031731345726372255 24-25 0.018162020251278856 26-27 0.014404360888945299 28-29 0.018162020251278856 30-31 0.013694580787171182 32-33 0.008767871845444966 34-35 0.010604949755919147 36-37 0.006471524457352235 38-39 0.009185389552370917 40-41 0.008016339972978254 42-43 0.0062627656038892615 44-45 0.0019205814518593732 46-47 0.003757659362333557 48-49 0.0014195602035482324 50-51 0.006972545705663377 52-53 0.006304517374581856 54-55 0.009185389552370917 56-57 0.02434128231378293 58-59 0.009602907259296866 60-61 0.021669168989456842 62-63 0.014404360888945299 64-65 0.01219151704223776 66-67 0.0177862543150455 68-69 0.028057189905423892 70-71 0.01991559462036785 72-73 0.018537786187512215 74-75 0.01966508399621228 76-77 0.02780667928126832 78-79 0.03937191976311715 80-81 0.03340141655407606 82-83 0.03281689176437973 84-85 0.02409077168962736 86-87 0.02655412616049047 88-89 0.02229544554984577 90-91 0.019247566289286328 92-93 0.03419470019723536 94-95 0.025552083663868186 96-97 0.039789437470043106 98-99 0.05757569178508861 100-101 0.06763786852200401 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1197554.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.50868460776152 #Duplication Level Percentage of deduplicated Percentage of total 1 83.74960231175729 55.70075886178121 2 10.201457902117452 13.569710923025724 3 2.716528821989727 5.42018275948826 4 1.0634858907635132 2.8292419077437923 5 0.5698811266598559 1.8951022058468077 6 0.3352277514303176 1.3377334074988843 7 0.24029115578855556 1.1187014086062872 8 0.163399687510652 0.8693998625322195 9 0.13125242782661004 0.7856483693690689 >10 0.745869752574495 9.187468743196742 >50 0.0565844822765404 2.573261480423687 >100 0.025034859307585193 2.9508727076403156 >500 6.290136351759862E-4 0.297362252234706 >1k 6.290136351759862E-4 0.9822827352941611 >5k 1.2580272703519726E-4 0.48227237531818956 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5764 0.48131441254423596 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4383 0.36599602189128844 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2899 0.24207676647566623 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2083 0.17393787670535107 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1320 0.110224674628451 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 4.175177069259507E-5 14-15 8.350354138519014E-5 0.0 0.0 0.0 8.350354138519014E-5 16-17 8.350354138519014E-5 0.0 0.0 0.0 8.350354138519014E-5 18-19 8.350354138519014E-5 0.0 0.0 0.0 8.350354138519014E-5 20-21 8.350354138519014E-5 0.0 0.0 8.350354138519014E-5 8.350354138519014E-5 22-23 8.350354138519014E-5 0.0 0.0 1.6700708277038028E-4 8.350354138519014E-5 24-25 8.350354138519014E-5 0.0 0.0 4.5926947761854584E-4 8.350354138519014E-5 26-27 8.350354138519014E-5 0.0 0.0 7.097801017741162E-4 8.350354138519014E-5 28-29 8.350354138519014E-5 0.0 0.0 0.0012943048914704474 8.350354138519014E-5 30-31 8.350354138519014E-5 0.0 0.0 0.002755616865711275 8.350354138519014E-5 32-33 8.350354138519014E-5 0.0 0.0 0.004968460712418814 8.350354138519014E-5 34-35 8.350354138519014E-5 0.0 0.0 0.007097801017741162 8.350354138519014E-5 36-37 8.350354138519014E-5 4.175177069259507E-5 0.0 0.008851375386830156 8.350354138519014E-5 38-39 8.350354138519014E-5 8.350354138519014E-5 0.0 0.010270935590378387 8.350354138519014E-5 40-41 8.350354138519014E-5 8.350354138519014E-5 0.0 0.012567282978471117 8.350354138519014E-5 42-43 8.350354138519014E-5 8.350354138519014E-5 0.0 0.016491949423575056 8.350354138519014E-5 44-45 8.350354138519014E-5 8.350354138519014E-5 0.0 0.022420700861923555 8.350354138519014E-5 46-47 8.350354138519014E-5 8.350354138519014E-5 0.0 0.03599002633701695 8.350354138519014E-5 48-49 8.350354138519014E-5 8.350354138519014E-5 0.0 0.06914093226693743 8.350354138519014E-5 50-51 8.350354138519014E-5 8.350354138519014E-5 0.0 0.09039258354946833 1.6700708277038028E-4 52-53 8.350354138519014E-5 8.350354138519014E-5 0.0 0.10755256130412491 1.6700708277038028E-4 54-55 8.350354138519014E-5 8.350354138519014E-5 0.0 0.13026552456089663 1.6700708277038028E-4 56-57 8.350354138519014E-5 8.350354138519014E-5 0.0 0.1490120696018718 1.6700708277038028E-4 58-59 8.350354138519014E-5 8.350354138519014E-5 0.0 0.17072299036202127 1.6700708277038028E-4 60-61 8.350354138519014E-5 8.350354138519014E-5 0.0 0.20951038533544208 1.6700708277038028E-4 62-63 8.350354138519014E-5 8.350354138519014E-5 0.0 0.23944640492203273 1.6700708277038028E-4 64-65 8.350354138519014E-5 8.350354138519014E-5 0.0 0.2753946794883571 1.6700708277038028E-4 66-67 8.350354138519014E-5 8.350354138519014E-5 0.0 0.34102846301711653 1.6700708277038028E-4 68-69 8.350354138519014E-5 8.350354138519014E-5 0.0 0.4176847140087211 1.6700708277038028E-4 70-71 8.350354138519014E-5 8.350354138519014E-5 0.0 0.4786840509906025 1.6700708277038028E-4 72-73 8.350354138519014E-5 8.350354138519014E-5 0.0 0.5500378271042474 1.6700708277038028E-4 74-75 8.350354138519014E-5 8.350354138519014E-5 0.0 0.6128742419966031 1.6700708277038028E-4 76-77 8.350354138519014E-5 8.350354138519014E-5 0.0 0.6614733030827837 1.6700708277038028E-4 78-79 8.350354138519014E-5 8.350354138519014E-5 0.0 0.7234329307905948 1.6700708277038028E-4 80-81 8.350354138519014E-5 8.350354138519014E-5 0.0 0.8303174637636382 1.6700708277038028E-4 82-83 8.350354138519014E-5 8.350354138519014E-5 0.0 0.9538192014723343 1.6700708277038028E-4 84-85 8.350354138519014E-5 8.350354138519014E-5 0.0 1.0853372791540088 1.6700708277038028E-4 86-87 8.350354138519014E-5 8.350354138519014E-5 0.0 1.290922998044347 1.6700708277038028E-4 88-89 8.350354138519014E-5 8.350354138519014E-5 0.0 1.5351291048253355 1.6700708277038028E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7755 0.0 27.948238 1 GGTATCA 5360 0.0 27.223562 1 GTACATG 5235 0.0 24.060274 1 TCAACGC 9300 0.0 23.254116 4 ATCAACG 9280 0.0 23.253014 3 CAACGCA 9310 0.0 23.179054 5 TATCAAC 9335 0.0 23.16596 2 TACATGG 5325 0.0 22.849335 2 AACGCAG 9520 0.0 22.763802 6 GTACCTG 930 0.0 21.976418 1 GAGTACT 4605 0.0 21.929468 12-13 TACCTGG 915 0.0 21.816315 2 ACATGGG 5410 0.0 21.261284 3 AGAGTAC 7565 0.0 21.200912 10-11 ACGCAGA 10485 0.0 20.714035 7 CGCAGAG 10495 0.0 20.603731 8 AGTACTT 4895 0.0 20.24194 12-13 GTACTTT 4885 0.0 20.089651 14-15 CCTATAC 405 0.0 19.951027 3 TACCGGG 120 0.004747167 19.803501 2 >>END_MODULE