FastQCFastQC Report
Thu 26 May 2016
SRR936874_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936874_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197554
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61630.5146323255569268No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50630.4227784300332177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33330.27831730343683875No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23050.19247566289286328No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13530.11298029149416228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61300.027.5139731
GTATCAA86000.026.4067461
GAGTACT54000.022.7800912-13
TAACGCA1050.0021978422.6162744
ATCAACG100200.022.5156443
CAACGCA101050.022.3723185
TCAACGC101200.022.3391574
TATCAAC101600.022.2072432
GTCTAGC2606.2391337E-1021.9277151
AACGCAG103950.021.7472656
GTACTTT57950.020.86027514-15
AGGACCG1604.9942246E-520.7787045
AGTACTT56550.020.4510612-13
GTACATG50550.020.3950141
TACATGG50450.020.0545622
TATAACG1200.0047635419.7917212
AGAGTAC85450.019.78609710-11
GGACCGA1456.153051E-419.6519416
ACATGGG49450.019.305823
ACGCAGA116900.019.2975127
Produced by FastQC (version 0.11.5)