##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936874_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1197554 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.151806933132036 33.0 31.0 34.0 31.0 34.0 2 32.22884563034318 33.0 31.0 34.0 30.0 34.0 3 32.36428336425748 34.0 31.0 34.0 31.0 34.0 4 36.05033092453451 37.0 35.0 37.0 35.0 37.0 5 35.961989187961464 37.0 35.0 37.0 35.0 37.0 6 35.997581737441486 37.0 35.0 37.0 35.0 37.0 7 35.94932086569791 37.0 35.0 37.0 35.0 37.0 8 35.93083902688313 37.0 35.0 37.0 35.0 37.0 9 37.614191092844244 39.0 37.0 39.0 35.0 39.0 10-11 37.51333635059463 39.0 37.0 39.0 35.0 39.0 12-13 37.44100349545825 39.0 37.0 39.0 34.5 39.0 14-15 38.647217996015215 40.0 38.0 41.0 34.0 41.0 16-17 38.56624335938088 40.0 38.0 41.0 34.0 41.0 18-19 38.609917381596155 40.0 38.0 41.0 34.0 41.0 20-21 38.71350561227302 40.0 38.0 41.0 34.0 41.0 22-23 38.64961914034774 40.0 38.0 41.0 34.0 41.0 24-25 38.56940104579835 40.0 38.0 41.0 34.0 41.0 26-27 38.40321021014501 40.0 38.0 41.0 34.0 41.0 28-29 38.246598065723965 40.0 38.0 41.0 34.0 41.0 30-31 38.19264392252876 40.0 38.0 41.0 34.0 41.0 32-33 38.10834584494728 40.0 37.0 41.0 33.5 41.0 34-35 37.98904433537027 40.0 37.0 41.0 33.0 41.0 36-37 37.81332616316258 40.0 37.0 41.0 33.0 41.0 38-39 37.67813142455371 40.0 37.0 41.0 33.0 41.0 40-41 37.456601956989 40.0 37.0 41.0 32.5 41.0 42-43 37.22730290241609 40.0 36.5 41.0 32.0 41.0 44-45 36.98943554946165 40.0 36.0 41.0 31.0 41.0 46-47 36.62594755643587 39.0 36.0 41.0 30.5 41.0 48-49 36.2619719027284 39.0 36.0 41.0 30.0 41.0 50-51 36.01981998306548 39.0 35.0 40.5 29.0 41.0 52-53 35.809053286949904 39.0 35.0 40.0 28.5 41.0 54-55 35.55142732603289 39.0 35.0 40.0 28.0 41.0 56-57 35.29660666658873 38.0 35.0 40.0 27.5 41.0 58-59 34.961375019414575 38.0 34.0 40.0 26.5 41.0 60-61 34.63099618054802 38.0 34.0 40.0 26.0 41.0 62-63 34.30215589443148 37.0 34.0 40.0 26.0 41.0 64-65 33.89235266217641 37.0 33.0 39.5 25.5 41.0 66-67 33.45945527299813 36.0 33.0 39.0 24.5 40.0 68-69 33.02338266165868 36.0 32.0 39.0 23.0 40.0 70-71 32.61955995303761 35.0 32.0 38.0 22.0 40.0 72-73 32.217670351399605 35.0 31.5 37.5 21.5 39.5 74-75 31.73646532849458 35.0 31.0 37.0 20.5 39.0 76-77 30.2782425677673 33.5 29.5 35.5 19.5 38.0 78-79 30.9904993845789 34.0 31.0 36.0 19.5 38.0 80-81 30.70352652155978 34.0 31.0 36.0 17.5 37.5 82-83 30.475597760101007 34.0 30.5 35.0 17.5 37.0 84-85 30.115344694268487 34.0 30.0 35.0 13.5 36.5 86-87 29.74377689857827 34.0 30.0 35.0 7.5 36.0 88-89 29.360384583910204 34.0 29.5 35.0 4.5 36.0 90-91 28.887797126476134 34.0 29.0 35.0 2.0 35.0 92-93 28.4378963286833 34.0 29.0 35.0 2.0 35.0 94-95 27.920695434193362 33.0 28.0 35.0 2.0 35.0 96-97 27.266076101787476 33.0 26.5 35.0 2.0 35.0 98-99 26.458492477165954 33.0 25.5 34.5 2.0 35.0 100-101 23.14939869099849 29.0 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29697307093839953 1101 2 0.2415158872005776 1101 3 0.2332623124006048 1101 4 0.16469578723346245 1101 5 0.2416185931394068 1101 6 0.2372768429722072 1101 7 0.18090780626016567 1101 8 0.185925632673289 1101 9 0.2559563151161228 1101 10-11 0.3343341762229315 1101 12-13 0.26835533740658946 1101 14-15 0.3357689063949181 1101 16-17 0.37488728169648056 1101 18-19 0.3440370758443194 1101 20-21 0.2641019231604673 1101 22-23 0.2911506719708825 1101 24-25 0.28633472236007407 1101 26-27 0.23951061162752296 1101 28-29 0.22889053088622546 1101 30-31 0.23643861223794715 1101 32-33 0.3363810699747489 1101 34-35 0.48413647569434204 1101 36-37 0.5860923427131439 1101 38-39 0.41844674868143983 1101 40-41 0.5860107381369559 1101 42-43 0.6169954078012339 1101 44-45 0.5837052955012112 1101 46-47 0.3840406666817273 1101 48-49 0.3498249193783849 1101 50-51 0.25297637609926227 1101 52-53 0.24242581199688829 1101 54-55 0.4762889530873977 1101 56-57 0.3577954662398284 1101 58-59 0.42412857076059396 1101 60-61 0.18033872609595392 1101 62-63 0.38520934961454145 1101 64-65 0.38959951885021127 1101 66-67 0.25569318412946274 1101 68-69 0.2917615077650524 1101 70-71 0.46322355054199704 1101 72-73 0.5414178053424621 1101 74-75 0.2986860634024744 1101 76-77 0.5377878083662395 1101 78-79 0.607504515736796 1101 80-81 0.8244626875448766 1101 82-83 0.641913248796925 1101 84-85 0.4731066803003472 1101 86-87 0.5367001636172368 1101 88-89 0.23074483420301206 1101 90-91 0.17820198156578826 1101 92-93 0.45476011745786593 1101 94-95 0.5277108946798776 1101 96-97 0.6949779350751193 1101 98-99 0.8689719960862377 1101 100-101 1.1355949645604646 1102 1 0.4103033602134687 1102 2 0.38853382923812774 1102 3 0.36477368733009996 1102 4 0.19365888250353436 1102 5 0.3199061760676116 1102 6 0.24755524000693896 1102 7 0.3149876053523215 1102 8 0.33142669361712507 1102 9 0.3638905634575593 1102 10-11 0.46793890078042466 1102 12-13 0.4318545047671165 1102 14-15 0.608564426257395 1102 16-17 0.5434255965416384 1102 18-19 0.5496929808697004 1102 20-21 0.4468706508336453 1102 22-23 0.46392537584668503 1102 24-25 0.3381604858367453 1102 26-27 0.5582940179287235 1102 28-29 0.5332947907771768 1102 30-31 0.6178566509691166 1102 32-33 0.4735332415522038 1102 34-35 0.5299551483058735 1102 36-37 0.5652639335917158 1102 38-39 0.6134452042695244 1102 40-41 0.5956277239820835 1102 42-43 0.5490396048413402 1102 44-45 0.6112918497167783 1102 46-47 0.6857949171719113 1102 48-49 0.7825667877139111 1102 50-51 0.7623747940842804 1102 52-53 0.7782994791022588 1102 54-55 0.7773361315139695 1102 56-57 0.9193939997199934 1102 58-59 0.8474743713031572 1102 60-61 0.8246552902494813 1102 62-63 0.7430633556847539 1102 64-65 0.8442324188528971 1102 66-67 0.8466979919508972 1102 68-69 0.615754282603227 1102 70-71 0.5691718866060285 1102 72-73 0.803911090508052 1102 74-75 0.9754218480980263 1102 76-77 0.9506762615369837 1102 78-79 1.0206034789663612 1102 80-81 0.9937020311026679 1102 82-83 0.9909097167940253 1102 84-85 0.7501275097166946 1102 86-87 0.8485283444342961 1102 88-89 0.8343523789909568 1102 90-91 0.6776517008857113 1102 92-93 0.9604083004236728 1102 94-95 0.9766741242466352 1102 96-97 1.0540375896640377 1102 98-99 1.1748065157069831 1102 100-101 1.3134578342120982 1103 1 0.26561382814665535 1103 2 0.2051186819730475 1103 3 0.16004335454927343 1103 4 0.12495749272921586 1103 5 0.1775849979469939 1103 6 0.14384811263220598 1103 7 0.21744521086041857 1103 8 0.15231793421383344 1103 9 0.26697611745475314 1103 10-11 0.22929456825460193 1103 12-13 0.2152043071386629 1103 14-15 0.40341971122488474 1103 16-17 0.4929920889552477 1103 18-19 0.3877555844652605 1103 20-21 0.46452811608928357 1103 22-23 0.3592747319604257 1103 24-25 0.2981598483255894 1103 26-27 0.33103085332334814 1103 28-29 0.3292051567085821 1103 30-31 0.37531234394374025 1103 32-33 0.4119034442807461 1103 34-35 0.5285303108712185 1103 36-37 0.5527703087032805 1103 38-39 0.4460554936995891 1103 40-41 0.48001507576073976 1103 42-43 0.42113096019006235 1103 44-45 0.6120983013296879 1103 46-47 0.5338170388090404 1103 48-49 0.46229871427262736 1103 50-51 0.4653886280763757 1103 52-53 0.5225602849163593 1103 54-55 0.48263544300192507 1103 56-57 0.40702947084074026 1103 58-59 0.5666586757784842 1103 60-61 0.41372197391644505 1103 62-63 0.6083429043268467 1103 64-65 0.4518203953924882 1103 66-67 0.40138369895077375 1103 68-69 0.42753867633011566 1103 70-71 0.3104427650964041 1103 72-73 0.32595144496425377 1103 74-75 0.3044477310509812 1103 76-77 0.3737222898424797 1103 78-79 0.7376967468485525 1103 80-81 0.9038958344942252 1103 82-83 0.5394577851352196 1103 84-85 0.26476317209078815 1103 86-87 0.23425128208570456 1103 88-89 0.2381694770401701 1103 90-91 0.3064847367138377 1103 92-93 0.24087017725651805 1103 94-95 0.4444479352883306 1103 96-97 0.9906498553786882 1103 98-99 1.0462300781193221 1103 100-101 1.1102113086479015 1104 1 0.10101303763282488 1104 2 0.12532737762522572 1104 3 0.11821884862042253 1104 4 0.09976855992289302 1104 5 0.03285456316438484 1104 6 0.12717459484564841 1104 7 0.032942444061994536 1104 8 0.06612346781066947 1104 9 0.21761959571562528 1104 10-11 0.09733567497396933 1104 12-13 0.11655450476712303 1104 14-15 0.1850418456122398 1104 16-17 0.19629011267066687 1104 18-19 0.18646072280518666 1104 20-21 0.16073839276912594 1104 22-23 0.21060279520152392 1104 24-25 0.29052822777222786 1104 26-27 0.2983778888964679 1104 28-29 0.17195930690620997 1104 30-31 0.14991471548524515 1104 32-33 0.13146549961671639 1104 34-35 0.011858374112321712 1104 36-37 0.15068651423687385 1104 38-39 0.2311258494308177 1104 40-41 0.34522772398208446 1104 42-43 0.14486218548649532 1104 44-45 0.17129830132968493 1104 46-47 0.26491427201062123 1104 48-49 0.3006022715848786 1104 50-51 0.2924747940842778 1104 52-53 0.2330962532958054 1104 54-55 0.12708129280429858 1104 56-57 0.14294883842966755 1104 58-59 0.04340017775476923 1104 60-61 0.14422948379787215 1104 62-63 0.03020851697507254 1104 64-65 0.006184031756120589 1104 66-67 0.08619476614444466 1104 68-69 0.30444460518387473 1104 70-71 0.19187833821893463 1104 72-73 0.17035302599192192 1104 74-75 0.3121315255173833 1104 76-77 0.38240529379504906 1104 78-79 0.45232283380507354 1104 80-81 0.5091084827155683 1104 82-83 0.48264197485854154 1104 84-85 0.24229202584572818 1104 86-87 0.2225089895955854 1104 88-89 0.1926975402812765 1104 90-91 -0.028196686211064304 1104 92-93 0.14217926816560578 1104 94-95 0.39882896295631554 1104 96-97 0.6538182348253265 1104 98-99 1.046038773771496 1104 100-101 1.2419836406637081 1105 1 0.20615589477567653 1105 2 0.1416956315934783 1105 3 0.15176805496962942 1105 4 0.1264161789705085 1105 5 0.13686726157708762 1105 6 0.1674349123059642 1105 7 0.16886625358580432 1105 8 0.12487267415987446 1105 9 0.1673211830172079 1105 10-11 0.17150392894222222 1105 12-13 0.23284656825917693 1105 14-15 0.2737974011677906 1105 16-17 0.25942662060717225 1105 18-19 0.29407342121788105 1105 20-21 0.24660823403896615 1105 22-23 0.04820596980469816 1105 24-25 0.16746156110556143 1105 26-27 0.26798423810281946 1105 28-29 0.12469264023954452 1105 30-31 0.26440042977095857 1105 32-33 0.1468115313627436 1105 34-35 0.1539694852234348 1105 36-37 0.16729286344322247 1105 38-39 0.1834464843514496 1105 40-41 0.22975470810906984 1105 42-43 0.145512979137294 1105 44-45 0.08432052355190933 1105 46-47 0.14436506566141105 1105 48-49 0.07787528745789274 1105 50-51 0.1205097147191978 1105 52-53 0.16718196758152004 1105 54-55 0.09270351502652119 1105 56-57 -0.03625116157032693 1105 58-59 0.23502557458016327 1105 60-61 0.18849297586136515 1105 62-63 0.3092243899909448 1105 64-65 0.37985069842279273 1105 66-67 0.3064138137634913 1105 68-69 0.24172714486641667 1105 70-71 0.38684341758401075 1105 72-73 0.2928831847220792 1105 74-75 0.38357596996182153 1105 76-77 0.40569418268393775 1105 78-79 0.4359196592018968 1105 80-81 0.6155402287473173 1105 82-83 0.4776800700966355 1105 84-85 -0.04478416463046386 1105 86-87 -0.06976402627742928 1105 88-89 0.16956103234477027 1105 90-91 0.13033347251909433 1105 92-93 0.32185863324497177 1105 94-95 0.3785686454959958 1105 96-97 0.4932849014919931 1105 98-99 0.49153718646990896 1105 100-101 0.4200566565367261 1106 1 0.16666009645635427 1106 2 0.11792345605659449 1106 3 0.13090904469885345 1106 4 0.061682285413084514 1106 5 -0.04546700546306681 1106 6 0.09252361445349067 1106 7 0.020911071512976775 1106 8 0.004994056045966033 1106 9 0.2435490074803326 1106 10-11 0.07724155732690718 1106 12-13 0.027393720453396497 1106 14-15 0.04745753188674939 1106 16-17 0.07462736757263144 1106 18-19 0.06664111496204583 1106 20-21 0.10308349080834489 1106 22-23 0.1560537755936835 1106 24-25 0.1662850905173272 1106 26-27 0.1383857320337185 1106 28-29 0.26832009121993394 1106 30-31 0.11570295077936521 1106 32-33 0.19781059765593056 1106 34-35 0.27981915842605076 1106 36-37 0.15896886717805359 1106 38-39 0.2698003592347362 1106 40-41 0.22327478280561763 1106 42-43 0.17529355803551994 1106 44-45 0.06582379152576578 1106 46-47 0.07218486024591897 1106 48-49 0.3428846245260573 1106 50-51 0.031737539182316254 1106 52-53 0.14456291996247472 1106 54-55 0.18038325358860874 1106 56-57 -0.011741357648759276 1106 58-59 0.04172958951946981 1106 60-61 0.11848830732728288 1106 62-63 0.0022869483476171126 1106 64-65 -0.13260028196936702 1106 66-67 -0.03561699856143008 1106 68-69 0.04380931106622654 1106 70-71 0.10845873037579423 1106 72-73 0.01758439854094007 1106 74-75 -0.03979004311007017 1106 76-77 -0.024763333655933195 1106 78-79 -0.08035951913610262 1106 80-81 0.34519475722537507 1106 82-83 0.3501870728977572 1106 84-85 0.12520182976729544 1106 86-87 0.26140310724264637 1106 88-89 -0.0019142244246026507 1106 90-91 0.06954449025952414 1106 92-93 0.185504758361688 1106 94-95 0.23347994334847044 1106 96-97 0.4375986269821901 1106 98-99 0.7492309306342406 1106 100-101 0.36604638576174864 1107 1 0.1438552239299753 1107 2 0.12644947154362285 1107 3 0.08729721659578615 1107 4 0.16830004568116408 1107 5 0.13160313899194875 1107 6 0.13084449476866666 1107 7 0.023930588788708462 1107 8 0.16706080422329705 1107 9 0.16138988055011083 1107 10-11 0.18229702986234742 1107 12-13 0.20137913910122052 1107 14-15 0.23352283098559923 1107 16-17 0.2328603975051564 1107 18-19 0.3063612616812392 1107 20-21 0.23029990162208946 1107 22-23 0.20926484293055125 1107 24-25 0.14428989058978203 1107 26-27 0.15957850475481905 1107 28-29 0.14280272492006674 1107 30-31 0.22503773319117215 1107 32-33 0.12715171978607742 1107 34-35 0.17451734254957785 1107 36-37 0.11379475134233985 1107 38-39 0.15878374011595753 1107 40-41 0.21652841682273305 1107 42-43 0.23144263198380344 1107 44-45 0.2253392559101286 1107 46-47 0.2483085753208556 1107 48-49 0.38557809914453856 1107 50-51 0.3597862644460932 1107 52-53 0.2668108029493865 1107 54-55 0.2179986138204626 1107 56-57 0.35886046275607697 1107 58-59 0.37923174049533515 1107 60-61 0.3229306385322843 1107 62-63 0.33132014130147525 1107 64-65 0.37763745746820376 1107 66-67 0.5072358746894778 1107 68-69 0.5036227422123574 1107 70-71 0.3085945814829856 1107 72-73 0.4185063747217157 1107 74-75 0.32610227224563104 1107 76-77 0.40772138309450767 1107 78-79 0.5378707937742817 1107 80-81 0.6925261886432068 1107 82-83 0.6271494421410644 1107 84-85 0.6963271297718236 1107 86-87 0.5521136085332294 1107 88-89 0.43157760186711513 1107 90-91 0.3779735986234236 1107 92-93 0.6718870433773105 1107 94-95 0.6580382008316015 1107 96-97 0.5930539546097755 1107 98-99 0.8244507830093752 1107 100-101 0.5081674743819562 1108 1 -0.021473618568116137 1108 2 -0.011330707147145347 1108 3 -0.07907094729794295 1108 4 0.07746275144565118 1108 5 0.00862724762278333 1108 6 -0.026713003270522506 1108 7 0.012246682680519427 1108 8 0.03320164677456461 1108 9 -0.009305365037910462 1108 10-11 0.10360317889861648 1108 12-13 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-0.8167393920564905 2316 76-77 -1.1039567590820916 2316 78-79 -1.0467477727268069 2316 80-81 -0.942388873427511 2316 82-83 -1.1022003268521878 2316 84-85 -1.141749653781023 2316 86-87 -1.096037200140028 2316 88-89 -0.9782567365460899 2316 90-91 -1.0838010407989351 2316 92-93 -0.9250636556291028 2316 94-95 -0.9559150494853661 2316 96-97 -1.1089183126085764 2316 98-99 -1.1002436523560277 2316 100-101 -1.627659283130999 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 5.0 5 14.0 6 49.0 7 139.0 8 309.0 9 565.0 10 1064.0 11 1685.0 12 2546.0 13 3624.0 14 4760.0 15 6462.0 16 8645.0 17 12073.0 18 10803.0 19 6671.0 20 5757.0 21 6001.0 22 6231.0 23 7040.0 24 8164.0 25 9762.0 26 11759.0 27 14698.0 28 18696.0 29 23106.0 30 29190.0 31 36535.0 32 46580.0 33 60471.0 34 82807.0 35 116876.0 36 178303.0 37 265803.0 38 195983.0 39 14375.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.603330359797795 19.97823305168272 15.426134370395614 21.992302218123864 2 15.407278493961869 21.325992318824724 39.654422270898806 23.61230691631461 3 19.37300881445387 25.282075050210235 30.497759851018607 24.847156284317286 4 12.954844549547746 17.722821221959464 37.64362633104867 31.67870789744412 5 13.473295159901696 37.82760495258105 34.182312234398346 14.516787653118913 6 30.84779475497556 37.17385604323479 17.454578248663527 14.523770953126123 7 27.510993241223357 33.5331016388405 21.103599503654948 17.852305616281186 8 26.177924781491562 34.70858443606994 19.931009201278275 19.182481581160218 9 25.99400611079426 16.996408509686 20.86604935230424 36.1435360272155 10-11 24.45234202382523 27.215014938783554 27.49629661793957 20.836346419451647 12-13 25.752191661228487 24.657582289849156 28.113970976589297 21.476255072333068 14-15 22.586789405738696 25.778169502168584 27.026756204730646 24.60828488736207 16-17 21.405139728621727 29.248839404669603 27.422323929578095 21.923696937130575 18-19 21.297052506405052 27.45387860628714 30.283667865839604 20.965401021468203 20-21 22.19198409394659 26.303942735535557 30.484709588015015 21.01936358250284 22-23 21.850037660097165 26.476342611690164 30.351073939045754 21.32254578916692 24-25 21.83824874132876 26.52445567531501 30.515668985121415 21.121626598234812 26-27 21.709724467099438 26.633858867730094 30.381981486948213 21.27443517822226 28-29 21.329545274237606 26.898058953757452 30.539055173214347 21.233340598790594 30-31 21.544871644942816 26.521240481544474 30.435000517897535 21.498887355615178 32-33 21.41936809160726 26.750140078009466 30.321273403048068 21.50921842733521 34-35 21.970237871556876 26.622532870700923 30.188769055403153 21.21846020233905 36-37 21.878180198697038 26.3825838799917 30.215797295779307 21.523438625531956 38-39 21.5823255744038 26.789351451698973 30.281454312840772 21.346868661056455 40-41 21.821144987423043 26.648616868822177 30.223069779560856 21.307168364193924 42-43 21.601250970796762 26.791694155177165 29.86496530184472 21.74208957218135 44-45 21.81757905531116 26.791124293955153 29.62156664295091 21.76973000778278 46-47 22.183615058463964 27.043399657211808 29.180377150515746 21.592608133808476 48-49 22.114199083698423 27.312517457787123 28.799993987632057 21.773289470882396 50-51 21.91137996370824 27.412679736488084 28.85416240437505 21.821777895428625 52-53 22.227575892450798 27.577896597710094 28.498551586324034 21.695975923515075 54-55 21.958958994066137 27.387740139319632 28.676972845864917 21.976328020749307 56-57 22.136839173826107 27.72193434147959 28.384206181899714 21.757020302794594 58-59 22.374376223337098 27.34989278055754 28.47110419324885 21.804626802856514 60-61 22.165899060041994 27.370217697998463 28.920628598221292 21.543254643738255 62-63 22.008569133416948 27.406739069803947 28.911180623170228 21.673511173608873 64-65 21.898852571275867 27.430728375060543 28.73432097940641 21.936098074257178 66-67 21.95029078251372 27.621938057416656 28.4162717961629 22.01149936390673 68-69 21.872269144743626 27.455332445681425 28.60998971643154 22.06240869314341 70-71 22.023902971838183 27.411507898068322 28.573921844363458 21.990667285730034 72-73 22.0025521921627 27.378983833332498 28.589061985866067 22.029401988638735 74-75 21.921776596779655 27.847593342856104 28.167849079797126 22.062780980567116 76-77 22.087080692436807 27.57622065786507 28.29567185242712 22.041026797271005 78-79 21.9976791759279 27.623527093053017 28.215967753990977 22.1628259770281 80-81 22.14216078209515 27.757947981403735 28.014638189139934 22.08525304736118 82-83 22.09860228186098 27.63619662000647 28.315201620058666 21.949999478073885 84-85 22.020262328894383 27.719684270078098 28.07833542442126 22.181717976606258 86-87 22.021345175248882 27.53900867935809 28.23116953389993 22.208476611493094 88-89 21.98412151653504 27.59269581410108 28.046921482297975 22.376261187065904 90-91 21.895029282861124 27.571315781013382 28.251792106090512 22.281862830034985 92-93 22.053739628842838 27.586944032492955 28.14524964260425 22.214066696059962 94-95 21.91811421022658 27.38170391784533 28.137249007553784 22.562932864374314 96-97 21.651594941845925 27.499694167641227 28.343165199993486 22.505545690519362 98-99 21.68898096572963 27.53311864510098 27.989425080612467 22.788475308556926 100-101 21.549035088854662 27.64892857933164 28.0219415462711 22.780094785542595 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 421.0 1 306.0 2 226.5 3 296.0 4 266.0 5 227.5 6 423.0 7 983.0 8 1662.0 9 1970.5 10 1717.0 11 1382.5 12 1380.5 13 1529.5 14 1689.5 15 1847.5 16 1962.0 17 2038.0 18 2160.0 19 2363.0 20 2634.0 21 2923.5 22 3373.0 23 4055.0 24 4836.5 25 5989.0 26 7590.5 27 9391.5 28 11045.5 29 12980.5 30 15361.5 31 17536.0 32 19803.5 33 22312.5 34 25081.5 35 27557.5 36 30645.5 37 34012.5 38 35853.5 39 36749.0 40 37540.5 41 39018.0 42 40628.5 43 41983.5 44 43380.5 45 44556.5 46 44345.5 47 45359.0 48 49633.5 49 49396.0 50 44671.5 51 41387.5 52 38920.5 53 37498.5 54 35974.5 55 32895.5 56 29617.5 57 26418.0 58 23761.0 59 21398.5 60 18362.0 61 14614.5 62 11103.0 63 8513.0 64 6328.0 65 4644.5 66 3349.5 67 2454.5 68 1723.0 69 1113.0 70 736.0 71 544.0 72 412.0 73 291.5 74 214.5 75 156.5 76 105.5 77 50.5 78 28.0 79 23.5 80 14.0 81 5.0 82 3.5 83 3.0 84 0.5 85 0.0 86 1.0 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027222154491571986 2 0.006763786852200402 3 0.007431815183281923 4 1.6700708277038028E-4 5 0.0030896310312520355 6 0.0 7 0.0 8 2.5051062415557045E-4 9 8.350354138519014E-5 10-11 0.0 12-13 2.922623948481655E-4 14-15 0.0 16-17 4.175177069259507E-5 18-19 0.005177219565881789 20-21 0.0018788296811667784 22-23 0.0 24-25 0.005093716024496599 26-27 0.006221013833196665 28-29 0.008934878928215345 30-31 0.0339024378023872 32-33 5.84524789696331E-4 34-35 0.004926708941726218 36-37 0.016992970671886196 38-39 0.007932836431593064 40-41 0.010521446214533959 42-43 0.007014297476355973 44-45 0.003173134572637225 46-47 0.001795326139781588 48-49 0.0018788296811667784 50-51 0.0030896310312520355 52-53 0.0030896310312520355 54-55 0.001670070827703803 56-57 7.932836431593063E-4 58-59 8.350354138519015E-4 60-61 4.175177069259507E-5 62-63 0.0 64-65 0.007849332890207873 66-67 7.932836431593063E-4 68-69 0.0010437942673148768 70-71 0.0038411629037187465 72-73 0.0126925382905489 74-75 0.006137510291811476 76-77 0.0036741558209483663 78-79 0.011231226316308074 80-81 1.2525531207778522E-4 82-83 0.0055529855021151445 84-85 9.602907259296866E-4 86-87 0.0 88-89 0.001795326139781588 90-91 8.767871845444966E-4 92-93 1.6700708277038028E-4 94-95 6.680283310815211E-4 96-97 2.0875885346297536E-4 98-99 2.922623948481655E-4 100-101 7.097801017741162E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1197554.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.88267144668171 #Duplication Level Percentage of deduplicated Percentage of total 1 82.91316104567868 53.79627386732578 2 10.634458787914786 13.799841910991043 3 2.825615641863885 5.500004739769774 4 1.1849453690826408 3.0752968425782394 5 0.6204231583376336 2.0127355970166634 6 0.3713427945723085 1.445622752059663 7 0.2456082944659973 1.115500559209201 8 0.18357763669483157 0.9528805989303246 9 0.1349478644348642 0.7880200155502775 >10 0.795989742055439 9.5334639279867 >50 0.06157329613499958 2.762424721023751 >100 0.02642298622588533 3.1057248294295197 >500 0.0011600295228666685 0.4924056835453176 >1k 5.155686768296304E-4 0.6809883629601767 >5k 2.577843384148152E-4 0.9388155916235963 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6163 0.5146323255569268 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5063 0.4227784300332177 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3333 0.27831730343683875 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2305 0.19247566289286328 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1353 0.11298029149416228 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.175177069259507E-5 0.0 14-15 0.0 0.0 0.0 8.350354138519014E-5 0.0 16-17 0.0 0.0 0.0 8.350354138519014E-5 0.0 18-19 0.0 0.0 0.0 8.350354138519014E-5 0.0 20-21 0.0 0.0 0.0 8.350354138519014E-5 0.0 22-23 0.0 0.0 0.0 1.6700708277038028E-4 4.175177069259507E-5 24-25 0.0 0.0 0.0 3.3401416554076056E-4 8.350354138519014E-5 26-27 0.0 0.0 0.0 5.42773019003736E-4 8.350354138519014E-5 28-29 0.0 0.0 0.0 0.001127297808700067 8.350354138519014E-5 30-31 0.0 0.0 0.0 0.0026721133243260845 8.350354138519014E-5 32-33 0.0 0.0 0.0 0.0048849571710336236 8.350354138519014E-5 34-35 0.0 4.175177069259507E-5 0.0 0.007223056329818948 8.350354138519014E-5 36-37 0.0 8.350354138519014E-5 0.0 0.00906013424029313 8.350354138519014E-5 38-39 0.0 8.350354138519014E-5 0.0 0.010563197985226553 8.350354138519014E-5 40-41 0.0 8.350354138519014E-5 0.0 0.012943048914704474 8.350354138519014E-5 42-43 0.0 1.6700708277038028E-4 0.0 0.016909467130501003 8.350354138519014E-5 44-45 0.0 1.6700708277038028E-4 0.0 0.022963473880927288 8.350354138519014E-5 46-47 0.0 1.6700708277038028E-4 0.0 0.036407544043942905 8.350354138519014E-5 48-49 0.0 1.6700708277038028E-4 0.0 0.06930793934970782 8.350354138519014E-5 50-51 0.0 1.6700708277038028E-4 0.0 0.09047608709085353 8.350354138519014E-5 52-53 0.0 1.6700708277038028E-4 0.0 0.10763606484551011 1.6700708277038028E-4 54-55 0.0 1.6700708277038028E-4 0.0 0.13022377279020403 1.6700708277038028E-4 56-57 0.0 1.6700708277038028E-4 0.0 0.14880331074840883 1.6700708277038028E-4 58-59 0.0 1.6700708277038028E-4 0.0 0.17072299036202127 1.6700708277038028E-4 60-61 0.0 1.6700708277038028E-4 0.0 0.20992790304236802 1.6700708277038028E-4 62-63 0.0 1.6700708277038028E-4 0.0 0.23952990846341793 1.6700708277038028E-4 64-65 0.0 1.6700708277038028E-4 0.0 0.2753946794883571 1.6700708277038028E-4 66-67 0.0 1.6700708277038028E-4 0.0 0.34010992406187945 1.6700708277038028E-4 68-69 0.0 1.6700708277038028E-4 0.0 0.41630690557586547 1.6700708277038028E-4 70-71 0.0 1.6700708277038028E-4 0.0 0.47634595183181716 1.6700708277038028E-4 72-73 0.0 1.6700708277038028E-4 0.0 0.5468646925316103 2.5051062415557045E-4 74-75 0.0 2.5051062415557045E-4 0.0 0.6089078237808065 2.922623948481655E-4 76-77 0.0 2.5051062415557045E-4 0.0 0.656797104765213 3.3401416554076056E-4 78-79 0.0 2.5051062415557045E-4 0.0 0.7177964417470946 3.757659362333556E-4 80-81 0.0 2.5051062415557045E-4 0.0 0.8236371804528231 4.1751770692595073E-4 82-83 0.0 2.5051062415557045E-4 0.0 0.9467631522252858 4.1751770692595073E-4 84-85 4.175177069259507E-5 2.5051062415557045E-4 0.0 1.078030719282805 4.1751770692595073E-4 86-87 8.350354138519014E-5 2.5051062415557045E-4 0.0 1.2822386297402872 4.5926947761854584E-4 88-89 8.350354138519014E-5 2.5051062415557045E-4 0.0 1.5273215237058204 5.84524789696331E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6130 0.0 27.513973 1 GTATCAA 8600 0.0 26.406746 1 GAGTACT 5400 0.0 22.78009 12-13 TAACGCA 105 0.00219784 22.616274 4 ATCAACG 10020 0.0 22.515644 3 CAACGCA 10105 0.0 22.372318 5 TCAACGC 10120 0.0 22.339157 4 TATCAAC 10160 0.0 22.207243 2 GTCTAGC 260 6.2391337E-10 21.927715 1 AACGCAG 10395 0.0 21.747265 6 GTACTTT 5795 0.0 20.860275 14-15 AGGACCG 160 4.9942246E-5 20.778704 5 AGTACTT 5655 0.0 20.45106 12-13 GTACATG 5055 0.0 20.395014 1 TACATGG 5045 0.0 20.054562 2 TATAACG 120 0.00476354 19.791721 2 AGAGTAC 8545 0.0 19.786097 10-11 GGACCGA 145 6.153051E-4 19.651941 6 ACATGGG 4945 0.0 19.30582 3 ACGCAGA 11690 0.0 19.297512 7 >>END_MODULE