FastQCFastQC Report
Thu 26 May 2016
SRR936873_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936873_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1221873
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57230.4683792832806683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43120.3529008333926685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28260.23128426604074234No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20980.17170360585756458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80450.027.877071
GGTATCA57400.027.8136861
GTACATG55500.024.3140071
ATCAACG93150.023.8732723
GAGTACT45000.023.7541212-13
TCAACGC93850.023.6445774
CAACGCA94200.023.5062855
TATCAAC95100.023.3837592
TACATGG55900.023.2059752
AACGCAG96100.023.1394826
ACATGGG55450.022.623063
GTGTAGC6800.021.6612721
GTACTTT49600.021.59635514-15
ACGCAGA105100.020.7502527
AGTACTT47800.020.57365212-13
CGCAGAG106250.020.4362248
AGAGTAC76850.019.84444610-11
CATGGGA31850.019.8422624
GCAGAGT110150.018.806829
CATGGGG24950.018.4735414
Produced by FastQC (version 0.11.5)