##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936873_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1221873 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.672249898311854 33.0 31.0 34.0 30.0 34.0 2 31.792378585990523 33.0 31.0 34.0 30.0 34.0 3 31.79521030418055 33.0 31.0 34.0 30.0 34.0 4 35.37408388596851 37.0 35.0 37.0 33.0 37.0 5 35.36134933827002 37.0 35.0 37.0 33.0 37.0 6 35.46385426308626 37.0 35.0 37.0 33.0 37.0 7 35.41364855430965 37.0 35.0 37.0 33.0 37.0 8 35.36785246911913 37.0 35.0 37.0 33.0 37.0 9 36.946474797298904 39.0 37.0 39.0 33.0 39.0 10-11 36.82589598100621 39.0 37.0 39.0 33.0 39.0 12-13 36.72381417708714 39.0 37.0 39.0 32.0 39.0 14-15 37.86257123285317 40.0 38.0 41.0 32.5 41.0 16-17 37.778811709563925 40.0 37.5 41.0 32.0 41.0 18-19 37.72990400802702 40.0 37.0 41.0 32.0 41.0 20-21 37.61606648154104 40.0 37.0 41.0 32.0 41.0 22-23 37.49198443700777 40.0 37.0 41.0 32.0 41.0 24-25 37.604948304774716 40.0 37.0 41.0 32.0 41.0 26-27 37.54963077177415 40.0 37.0 41.0 32.0 41.0 28-29 37.45761793574292 40.0 37.0 41.0 32.0 41.0 30-31 37.267116140548154 40.0 37.0 41.0 31.0 41.0 32-33 37.063700564624966 39.5 36.0 41.0 31.0 41.0 34-35 36.87324869278558 39.0 36.0 41.0 30.0 41.0 36-37 36.67603138787746 39.0 36.0 41.0 30.0 41.0 38-39 36.511145593691 39.0 35.0 41.0 30.0 41.0 40-41 36.33159379084405 39.0 35.0 41.0 30.0 41.0 42-43 36.105249481738284 39.0 35.0 40.5 29.5 41.0 44-45 35.8353241294308 39.0 35.0 40.0 28.5 41.0 46-47 35.472687423324686 38.5 35.0 40.0 27.0 41.0 48-49 35.20137199201554 38.5 35.0 40.0 26.0 41.0 50-51 34.491287556071704 38.0 34.0 39.5 25.0 40.5 52-53 34.69063356011631 38.0 34.0 39.5 25.5 40.5 54-55 35.095989926940035 39.0 35.0 40.0 26.0 41.0 56-57 34.99987478240374 39.0 35.0 40.0 26.0 41.0 58-59 34.75126793046414 38.0 34.0 40.0 25.0 41.0 60-61 34.454731383703546 38.0 34.0 40.0 24.0 41.0 62-63 34.123325010046045 38.0 34.0 40.0 23.0 41.0 64-65 33.78533489159675 37.0 33.5 40.0 22.5 41.0 66-67 33.4069768298342 36.5 33.0 39.5 22.0 41.0 68-69 33.00527878101897 36.0 32.5 39.0 21.0 41.0 70-71 32.604739608780946 35.5 32.0 39.0 20.0 40.5 72-73 32.19494333699165 35.0 32.0 38.0 18.5 40.0 74-75 31.74062402557385 35.0 31.0 37.0 17.5 39.5 76-77 31.29138134650655 35.0 31.0 37.0 13.5 39.0 78-79 30.85016855270556 35.0 31.0 36.0 9.5 39.0 80-81 30.3864558755288 34.5 30.0 36.0 7.0 37.5 82-83 29.964146437477545 34.0 30.0 35.5 4.5 37.0 84-85 29.556397432466383 34.0 29.5 35.0 2.0 37.0 86-87 29.203220792995673 34.0 29.0 35.0 2.0 36.0 88-89 28.836219066957042 34.0 29.0 35.0 2.0 36.0 90-91 28.43015231533883 34.0 29.0 35.0 2.0 35.5 92-93 28.04091914626152 34.0 28.5 35.0 2.0 35.0 94-95 27.606642834402592 34.0 27.0 35.0 2.0 35.0 96-97 27.072600834947657 34.0 26.0 35.0 2.0 35.0 98-99 26.408507676329698 33.0 25.0 35.0 2.0 35.0 100-101 23.877908342356367 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4463661900145617 1101 2 0.4756236673383505 1101 3 0.25865920980357515 1101 4 0.26536007979349563 1101 5 0.34886521336941456 1101 6 0.1577662781137903 1101 7 0.225699389518482 1101 8 0.2371717583625923 1101 9 0.34606245879044906 1101 10-11 0.437502549279003 1101 12-13 0.46163518730102027 1101 14-15 0.5452938267056382 1101 16-17 0.6683027348012303 1101 18-19 0.7446858018871509 1101 20-21 0.6291436964394279 1101 22-23 0.7035009634790086 1101 24-25 0.7244833314100774 1101 26-27 0.7548918342205155 1101 28-29 0.7951431412373253 1101 30-31 0.8383749052230556 1101 32-33 0.8484282966642454 1101 34-35 0.8995163122470231 1101 36-37 0.7751159246514163 1101 38-39 0.9285181829759139 1101 40-41 0.878990878308656 1101 42-43 0.8690799334390746 1101 44-45 0.9823536771459587 1101 46-47 0.8069557798831113 1101 48-49 0.6664571799932943 1101 50-51 0.8895041794253515 1101 52-53 0.9109910763126194 1101 54-55 0.9460532678978311 1101 56-57 0.7675766643841158 1101 58-59 0.841649243747888 1101 60-61 0.8477783674324684 1101 62-63 0.9159419069747088 1101 64-65 0.8953673572110787 1101 66-67 1.1221312065093656 1101 68-69 1.281671740992607 1101 70-71 1.1357868917860046 1101 72-73 1.00203370333643 1101 74-75 0.9317901486254279 1101 76-77 1.0629466527027525 1101 78-79 1.0606531517236313 1101 80-81 1.1631617695667202 1101 82-83 0.8845556889921653 1101 84-85 1.0604249380858697 1101 86-87 1.196609210046116 1101 88-89 1.1958318397099639 1101 90-91 1.1913908576781864 1101 92-93 1.1607189578388066 1101 94-95 1.3048485506444791 1101 96-97 1.300404842063287 1101 98-99 1.0829743236731133 1101 100-101 1.3983539666819738 1102 1 0.31599905413074936 1102 2 0.46966946853438785 1102 3 0.3086356370388188 1102 4 0.23501366483919384 1102 5 0.33781092236814203 1102 6 0.3585549730056883 1102 7 0.38730603407800857 1102 8 0.39213543175742416 1102 9 0.4862427958557163 1102 10-11 0.3802088181904679 1102 12-13 0.39549751802294253 1102 14-15 0.5224542363013782 1102 16-17 0.5988641564735957 1102 18-19 0.6370914729239843 1102 20-21 0.5611776036775353 1102 22-23 0.5723118492590302 1102 24-25 0.19264963022610004 1102 26-27 0.5528725182711014 1102 28-29 0.41500000076747057 1102 30-31 0.49681204174942906 1102 32-33 0.6778585866200828 1102 34-35 0.5709007807912769 1102 36-37 0.36806987003912894 1102 38-39 0.4651693197752067 1102 40-41 0.4553164582600431 1102 42-43 0.578856963241833 1102 44-45 0.4519011306014775 1102 46-47 0.4399700686606707 1102 48-49 0.28276946100663736 1102 50-51 0.32939687822172914 1102 52-53 0.49080022716604077 1102 54-55 0.5563868026645906 1102 56-57 0.7227458143133916 1102 58-59 0.49243840792912863 1102 60-61 0.3108510719036417 1102 62-63 0.4393724409610087 1102 64-65 0.5228938485945704 1102 66-67 0.516379740702142 1102 68-69 0.7647892373267382 1102 70-71 0.5105328438044197 1102 72-73 0.44842317161715783 1102 74-75 0.5498702064996372 1102 76-77 0.6405851598428107 1102 78-79 0.6382122406669595 1102 80-81 0.49133761300602075 1102 82-83 0.6230174884785455 1102 84-85 0.5621407321270517 1102 86-87 0.39800256620857155 1102 88-89 0.3495219340226825 1102 90-91 0.3722271746087742 1102 92-93 0.3187167673405469 1102 94-95 0.28008162500229616 1102 96-97 0.26097081945220424 1102 98-99 0.4247234589172457 1102 100-101 0.23048230937582304 1103 1 0.17257962913333813 1103 2 0.2990760092970781 1103 3 0.37678224603993726 1103 4 0.32414864234884533 1103 5 0.3018198056951178 1103 6 0.18112520861323844 1103 7 0.16085727914738612 1103 8 0.2113986875518492 1103 9 0.07607301307221093 1103 10-11 0.17456445560065958 1103 12-13 0.3092471294101742 1103 14-15 0.49200076948201854 1103 16-17 0.5890576692312379 1103 18-19 0.6112711884528821 1103 20-21 0.5990973546505387 1103 22-23 0.7180009695446827 1103 24-25 0.42715890249377253 1103 26-27 0.5680628499791425 1103 28-29 0.5393049250322619 1103 30-31 0.6194736423486518 1103 32-33 0.5465212471319134 1103 34-35 0.45306716083554477 1103 36-37 0.46688540458008987 1103 38-39 0.44243891668047297 1103 40-41 0.47413712183355017 1103 42-43 0.3170060777294452 1103 44-45 0.34885414420325134 1103 46-47 0.41253499158744944 1103 48-49 0.4704963157553266 1103 50-51 0.47976148553591713 1103 52-53 0.3289376012850198 1103 54-55 0.33778999315895675 1103 56-57 0.46878130826808473 1103 58-59 0.45729141720205035 1103 60-61 0.6685102522816138 1103 62-63 0.5745260561418917 1103 64-65 0.4058518836635088 1103 66-67 0.37141242352723225 1103 68-69 0.5746349465827407 1103 70-71 0.36140589434572234 1103 72-73 0.19964443230974638 1103 74-75 0.3712031121949657 1103 76-77 0.48474488395070736 1103 78-79 0.5296666296627599 1103 80-81 0.659680069720789 1103 82-83 0.5774776479639101 1103 84-85 0.5886182148870738 1103 86-87 0.5888281568015863 1103 88-89 0.544917741858427 1103 90-91 0.43414843674431225 1103 92-93 0.5957979936384454 1103 94-95 0.7668743661750455 1103 96-97 0.6380114463747724 1103 98-99 0.6062574331828827 1103 100-101 0.8551420682457049 1104 1 0.3361164053461714 1104 2 0.2605854312919398 1104 3 0.4135663872777684 1104 4 0.39706313632752455 1104 5 0.4216704589347202 1104 6 0.29323956793100336 1104 7 0.25607742656693233 1104 8 0.351024613193168 1104 9 0.4104303304413932 1104 10-11 0.6001371609067121 1104 12-13 0.503259475766832 1104 14-15 0.5070238777340279 1104 16-17 0.5129172291193669 1104 18-19 0.6022480533662744 1104 20-21 0.7593026759522132 1104 22-23 0.7507279018495225 1104 24-25 0.5783105948423781 1104 26-27 0.5341176877090845 1104 28-29 0.5666670714101372 1104 30-31 0.4030156757040615 1104 32-33 0.600407568437241 1104 34-35 0.6132810204198194 1104 36-37 0.5650875472011165 1104 38-39 0.6411406098219103 1104 40-41 0.6402896619116376 1104 42-43 0.6860910299821512 1104 44-45 0.6315585110469115 1104 46-47 0.7525592891002404 1104 48-49 0.9723512576523134 1104 50-51 0.771948225095116 1104 52-53 0.6434760400234509 1104 54-55 0.5143162416693343 1104 56-57 0.7971715858476571 1104 58-59 0.8599354793753875 1104 60-61 0.7760563254644595 1104 62-63 0.7939675426812869 1104 64-65 0.7925314919271003 1104 66-67 0.8021608527881696 1104 68-69 0.670648239469017 1104 70-71 0.7999961263064677 1104 72-73 1.0890904441389324 1104 74-75 0.860353089568509 1104 76-77 0.864534098148166 1104 78-79 0.875891245043622 1104 80-81 0.7859618793975933 1104 82-83 0.9218988560821089 1104 84-85 0.871529940022679 1104 86-87 0.8866666295711738 1104 88-89 0.9448813931696023 1104 90-91 0.762440219135204 1104 92-93 0.8022561066535978 1104 94-95 1.0537591371722073 1104 96-97 1.0763824274356395 1104 98-99 0.7959814270987451 1104 100-101 1.066513353025485 1105 1 0.269397410055749 1105 2 0.264779878421475 1105 3 0.1951847244021785 1105 4 0.3632071693349346 1105 5 0.3077737221118113 1105 6 0.3032019916558184 1105 7 0.2552576250567853 1105 8 0.37253522595197097 1105 9 0.48660335016190004 1105 10-11 0.37157558218925146 1105 12-13 0.3999580616704108 1105 14-15 0.38947351806141484 1105 16-17 0.34129514782686954 1105 18-19 0.42741039241904843 1105 20-21 0.23588327161754563 1105 22-23 0.290849538369109 1105 24-25 0.15875851236300065 1105 26-27 0.22382526328683383 1105 28-29 0.147166379473191 1105 30-31 -0.07960606176123264 1105 32-33 0.26652994400176766 1105 34-35 0.08388073581410538 1105 36-37 0.1913008840257433 1105 38-39 0.25888521350906757 1105 40-41 0.18219130775493397 1105 42-43 0.3319756264012099 1105 44-45 0.12132842824696155 1105 46-47 0.19973990605593883 1105 48-49 0.22755760903392286 1105 50-51 0.12473756914378953 1105 52-53 0.1891870098241597 1105 54-55 0.1856027549556316 1105 56-57 0.29864019295595057 1105 58-59 0.3114720565939564 1105 60-61 0.14607281938140915 1105 62-63 0.15832924155473904 1105 64-65 0.2655159732068668 1105 66-67 0.16935576120899754 1105 68-69 0.16941670182727364 1105 70-71 0.33851027889477336 1105 72-73 0.2251331398910068 1105 74-75 0.22311556370500085 1105 76-77 0.273979068054107 1105 78-79 0.11870513419421869 1105 80-81 0.23776825696390702 1105 82-83 0.43822024764011047 1105 84-85 0.5497009506176944 1105 86-87 0.41190397434420234 1105 88-89 0.5299862727016063 1105 90-91 0.5855937385920313 1105 92-93 0.30301258767396533 1105 94-95 0.3399053481597498 1105 96-97 0.30312606571474277 1105 98-99 0.23307634420702783 1105 100-101 0.32025079140272084 1106 1 0.1774849901051141 1106 2 0.2768260415451742 1106 3 0.19459755329734207 1106 4 0.1759984660187044 1106 5 0.3040199775226924 1106 6 0.33816125589746093 1106 7 0.2996348208447799 1106 8 0.28793375765798856 1106 9 0.3015541181231143 1106 10-11 0.38764038830071 1106 12-13 0.38535019675149584 1106 14-15 0.3638821354382529 1106 16-17 0.380114360163077 1106 18-19 0.30682119490250415 1106 20-21 0.3965075284981907 1106 22-23 0.049557615450879666 1106 24-25 0.2120610751634473 1106 26-27 0.29793638877542605 1106 28-29 0.48111597797510797 1106 30-31 0.29424431581053767 1106 32-33 0.20042314478011036 1106 34-35 0.03136188164006626 1106 36-37 0.20497977345446827 1106 38-39 0.007520437427558591 1106 40-41 0.05063629311838724 1106 42-43 -0.03497091295145793 1106 44-45 -0.012937827603387575 1106 46-47 -0.1277434859131148 1106 48-49 -0.16013523577956335 1106 50-51 -0.230502170010638 1106 52-53 -0.18615998266346168 1106 54-55 -0.2696959454552754 1106 56-57 -0.18594687072465632 1106 58-59 -0.2938378745324641 1106 60-61 -0.15134155251641346 1106 62-63 0.0904432284343315 1106 64-65 -0.18234726109270838 1106 66-67 -0.20141776652964438 1106 68-69 -0.18819857780533766 1106 70-71 -0.14921772294120217 1106 72-73 -0.08686040977194409 1106 74-75 0.03397688634148466 1106 76-77 0.013540606271501332 1106 78-79 0.016548348522658074 1106 80-81 -0.16494566985577208 1106 82-83 -0.17148980458271623 1106 84-85 -0.03450683226816054 1106 86-87 -0.13488660455035273 1106 88-89 -0.06974370183396772 1106 90-91 0.05593488378320899 1106 92-93 0.06626239052608085 1106 94-95 -0.0372336758910734 1106 96-97 0.08963504662496646 1106 98-99 -0.1702857902148729 1106 100-101 -0.022071793810333418 1107 1 0.12023359886968876 1107 2 0.23781389364075167 1107 3 0.3374124713220681 1107 4 0.2215174349037099 1107 5 0.4103162396229365 1107 6 0.4047199252037075 1107 7 0.20730007539199846 1107 8 0.2739524561499067 1107 9 0.35512132849373756 1107 10-11 0.2181551783833413 1107 12-13 0.2901166694465971 1107 14-15 0.4252649652968472 1107 16-17 0.4568949076881097 1107 18-19 0.3580010320910816 1107 20-21 0.3538120990408089 1107 22-23 0.42976418138662353 1107 24-25 0.15400929638457228 1107 26-27 0.2221906494151824 1107 28-29 0.15834995853318645 1107 30-31 0.24089294028309638 1107 32-33 0.17035884997849138 1107 34-35 0.2306358971633813 1107 36-37 0.24450662750574992 1107 38-39 0.2062401770827691 1107 40-41 0.35190126516177145 1107 42-43 0.44078154127056735 1107 44-45 0.40166591761077086 1107 46-47 0.3838954080052517 1107 48-49 0.12133947976809623 1107 50-51 0.23066914170163955 1107 52-53 0.391929663139571 1107 54-55 0.39009228459109835 1107 56-57 0.47243123834628165 1107 58-59 0.3401371078110529 1107 60-61 0.4183407266036028 1107 62-63 0.2733425738552171 1107 64-65 0.22668284906699654 1107 66-67 0.3046205129788717 1107 68-69 0.28458344938745483 1107 70-71 0.16503181331365369 1107 72-73 0.17728906305145387 1107 74-75 0.35300866322581115 1107 76-77 0.020461410472126573 1107 78-79 0.05563693793404312 1107 80-81 0.23375434489294022 1107 82-83 0.3084654336689745 1107 84-85 0.1441369694947312 1107 86-87 -0.040869846173862356 1107 88-89 -0.08501071951204153 1107 90-91 0.09849211898682952 1107 92-93 0.0022481742044071495 1107 94-95 -0.024430173131385402 1107 96-97 -0.03860082728431635 1107 98-99 0.03469256035127444 1107 100-101 -0.10012426805704067 1108 1 -0.11441631915915451 1108 2 0.09834498693765781 1108 3 0.23918497290340213 1108 4 0.1621660925600068 1108 5 0.260520258028599 1108 6 0.17676265582313277 1108 7 -0.009914689039213442 1108 8 0.19040055262355793 1108 9 0.20762488135955692 1108 10-11 0.31426797351177527 1108 12-13 0.27493088184870373 1108 14-15 0.36230325410053865 1108 16-17 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-2.0859508227586296 2316 86-87 -2.332526317348705 2316 88-89 -2.4013982110780887 2316 90-91 -2.4180535978183535 2316 92-93 -2.7618662887960106 2316 94-95 -2.168108451556357 2316 96-97 -2.1435739408655046 2316 98-99 -1.976006275482952 2316 100-101 -1.9330769330541742 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2611.0 3 1036.0 4 1058.0 5 1135.0 6 1323.0 7 1425.0 8 1849.0 9 2271.0 10 3032.0 11 3680.0 12 4507.0 13 5510.0 14 6173.0 15 7523.0 16 9264.0 17 12129.0 18 10997.0 19 7375.0 20 7258.0 21 7678.0 22 8352.0 23 9331.0 24 10506.0 25 11952.0 26 14197.0 27 16475.0 28 19566.0 29 23744.0 30 28948.0 31 35665.0 32 45341.0 33 58719.0 34 79176.0 35 111840.0 36 164311.0 37 238644.0 38 211528.0 39 35722.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.63061261410232 19.305321459298085 14.821942680138605 22.242123246460995 2 15.120015584100944 20.738804247649696 40.450809407427656 23.690370760821704 3 19.167580663643655 25.387480151259677 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26.648027294457748 29.7232372431681 21.586013595665037 24-25 21.905058098256543 26.796605973306924 29.90909295516439 21.389242973272143 26-27 21.810049739232205 26.898060149483683 29.818045663520536 21.473844447763575 28-29 21.440087998284557 27.092741942084164 29.989679516664186 21.477490542967093 30-31 21.696199195824768 26.75674967856725 29.851506662312694 21.695544463295285 32-33 21.481181091761435 26.96785540518034 29.794630277619667 21.75633322543856 34-35 22.087338117249598 26.90011719721869 29.555755431010777 21.45678925452093 36-37 21.966440047369897 26.726468299078547 29.60360855833626 21.703483095215294 38-39 21.66312974119199 27.105316354999832 29.703503888708383 21.528050015099794 40-41 21.926914513444757 26.928549302074156 29.61979195012892 21.524744234352166 42-43 21.73798287134357 27.01243707905216 29.307402052836206 21.94217799676807 44-45 21.91165103012144 27.02639109463728 29.159432198420372 21.902525676820908 46-47 22.123821379144967 27.296617569911113 28.794236389542938 21.78532466140098 48-49 22.16132272934668 27.420935576075145 28.504563710876994 21.91317798370118 50-51 21.90030698821725 27.617113426867483 28.536400323765765 21.9461792611495 52-53 22.217434467090115 27.74401796594883 28.16865501728093 21.86989254968012 54-55 21.908188807986615 27.509228874869102 28.38920991992025 22.193372397224035 56-57 22.077289131013902 27.870802579496207 28.11898844922911 21.93291984026078 58-59 22.362891299241568 27.4659760250569 28.208123850624332 21.9630088250772 60-61 22.15226781194563 27.492997170222473 28.59212941270351 21.762605605128382 62-63 22.039375553208476 27.57188999136146 28.595256617120196 21.793477838309876 64-65 21.880365803554913 27.673346657303878 28.38611360730952 22.060173931831688 66-67 21.913445471350865 27.845443194267837 28.061569640311735 22.179541694069556 68-69 21.829672517669035 27.732269678427468 28.30921188066296 22.128845923240533 70-71 22.024152329214346 27.609567770479604 28.227395206945992 22.138884693360055 72-73 22.048734249486255 27.545078791961636 28.281941102584053 22.124245855968056 74-75 21.797855359554703 28.09159742970573 27.91838906397086 22.192158146768715 76-77 22.034090485798224 27.752623958797678 27.956597075116385 22.256688480287714 78-79 22.012544637832416 27.85433485792635 27.881102209125046 22.252018295116187 80-81 22.082869510428186 27.986463399306384 27.75408454152462 22.17658254874081 82-83 22.0928280958459 27.88387932396663 27.957891599852303 22.065400980335166 84-85 22.032034301767805 27.941157209887134 27.850294852442808 22.176513635902257 86-87 22.057428408890924 27.79040595238583 28.016362512738453 22.1358031259848 88-89 22.074617098193038 27.777534490118406 27.759322021412135 22.388526390276425 90-91 22.044703457297672 27.717813518931923 28.007934110535622 22.229548913234787 92-93 22.163749536416578 27.814514234160182 27.88336609459312 22.13837013483012 94-95 21.982608496355656 27.611580202318347 27.886609393474686 22.519201907851304 96-97 21.89242407436891 27.726428295745187 28.031428256863013 22.349719373022893 98-99 21.781238372376226 27.843996533765697 27.712314014302926 22.662451079555154 100-101 21.823610057553257 27.79639271797185 27.74845555127537 22.63154167319952 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 283.0 1 203.5 2 178.5 3 244.0 4 225.5 5 230.0 6 426.0 7 923.5 8 1562.5 9 1889.0 10 1661.0 11 1376.5 12 1373.5 13 1487.0 14 1627.0 15 1748.0 16 1810.0 17 1875.0 18 1996.5 19 2105.0 20 2329.0 21 2722.5 22 3290.0 23 3965.5 24 4771.5 25 5948.0 26 7638.5 27 9605.0 28 11181.5 29 13085.5 30 15627.0 31 17816.0 32 20116.5 33 22768.5 34 25632.5 35 28169.5 36 31390.0 37 34681.0 38 36188.5 39 37188.0 40 38335.5 41 39987.0 42 41314.0 43 42740.0 44 44061.5 45 45051.0 46 45435.0 47 46253.5 48 50150.5 49 50005.0 50 45523.0 51 42657.5 52 40262.0 53 38666.0 54 36894.0 55 33670.0 56 30595.5 57 27582.5 58 24823.5 59 22253.5 60 18946.5 61 15087.0 62 11578.5 63 8906.0 64 6713.0 65 5028.5 66 3591.0 67 2577.0 68 1847.5 69 1240.0 70 836.5 71 602.5 72 433.5 73 296.5 74 221.5 75 170.5 76 116.0 77 64.0 78 42.5 79 27.5 80 20.5 81 18.0 82 12.0 83 9.0 84 6.0 85 6.0 86 7.0 87 4.5 88 1.5 89 1.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02226090600250599 2 0.009411780111353636 3 0.010884928302695943 4 0.02226090600250599 5 0.05647068066812181 6 0.04247577285036988 7 0.014649640347237397 8 0.04656785115965407 9 0.02766244937076112 10-11 0.022629193050341567 12-13 0.034537140930358555 14-15 0.01751409516373633 16-17 0.018168827693221798 18-19 0.01620463010476539 20-21 0.01493608582888729 22-23 0.0070792954750616465 24-25 0.0016777521068065176 26-27 4.092078309284189E-5 28-29 0.0025370885517561974 30-31 0.0 32-33 2.864454816498932E-4 34-35 8.184156618568378E-5 36-37 0.0 38-39 1.6368313237136755E-4 40-41 2.0460391546420946E-4 42-43 2.0460391546420946E-4 44-45 2.0460391546420946E-4 46-47 0.0 48-49 4.5012861402126086E-4 50-51 1.6368313237136755E-4 52-53 4.910493971141027E-4 54-55 0.002332484636291988 56-57 5.319701802069446E-4 58-59 0.001145781926599573 60-61 0.003723791261448612 62-63 0.0019232768053635686 64-65 0.0012276234927852568 66-67 0.011007690651974468 68-69 0.014199511733216135 70-71 0.027376003889111225 72-73 0.016900283417343703 74-75 0.018250669259407486 76-77 0.03179544846313815 78-79 0.01927368883672853 80-81 0.048409286398831956 82-83 0.03715607104830043 84-85 0.020051183715492527 86-87 0.014567798781051713 88-89 0.01493608582888729 90-91 0.025534568649933337 92-93 0.03355504213613035 94-95 0.015099768961258659 96-97 0.019110005704357162 98-99 0.030649666536538573 100-101 0.039488555684592426 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1221873.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.47696775518293 #Duplication Level Percentage of deduplicated Percentage of total 1 83.75441633266794 55.677396339009334 2 10.147086094883209 13.490950302772323 3 2.683816854481454 5.352360194885403 4 1.1215375378538155 2.982256589605414 5 0.5762985643337678 1.915529053928705 6 0.3497716461617662 1.3951055066143792 7 0.24671754758061318 1.1480724118607963 8 0.16489378762549245 0.8769311202407876 9 0.130559375107056 0.7811272232215736 >10 0.7415770470800634 9.110968635242802 >50 0.056532644267908515 2.580049294249222 >100 0.02531096017843725 2.928858816767776 >500 7.408038892547215E-4 0.33971805685931333 >1k 6.173365743789346E-4 0.9509479117593632 >5k 1.234673148757869E-4 0.4697285429828099 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5723 0.4683792832806683 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4312 0.3529008333926685 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2826 0.23128426604074234 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2098 0.17170360585756458 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 4.092078309284189E-5 0.0 0.0 0.0 0.0 20-21 8.184156618568378E-5 0.0 0.0 8.184156618568378E-5 4.092078309284189E-5 22-23 8.184156618568378E-5 0.0 0.0 2.864454816498932E-4 2.0460391546420946E-4 24-25 1.6368313237136755E-4 0.0 0.0 4.092078309284189E-4 2.4552469855705135E-4 26-27 2.0460391546420946E-4 0.0 0.0 9.820987942282054E-4 2.4552469855705135E-4 28-29 3.273662647427351E-4 0.0 0.0 0.0018005144560850432 2.864454816498932E-4 30-31 3.273662647427351E-4 0.0 0.0 0.002905375599591774 3.273662647427351E-4 32-33 3.273662647427351E-4 0.0 0.0 0.004869573188048185 3.273662647427351E-4 34-35 3.68287047835577E-4 0.0 0.0 0.006138117463926283 3.273662647427351E-4 36-37 4.092078309284189E-4 0.0 0.0 0.008716126798775323 3.273662647427351E-4 38-39 4.092078309284189E-4 0.0 0.0 0.010639403604138892 3.273662647427351E-4 40-41 4.092078309284189E-4 0.0 0.0 0.013299254505173615 3.273662647427351E-4 42-43 4.501286140212608E-4 0.0 0.0 0.01730949124827212 3.273662647427351E-4 44-45 4.910493971141027E-4 0.0 0.0 0.02447062828951945 3.273662647427351E-4 46-47 4.910493971141027E-4 0.0 0.0 0.03875198158892127 3.273662647427351E-4 48-49 4.910493971141027E-4 0.0 0.0 0.0696471728240169 3.273662647427351E-4 50-51 4.910493971141027E-4 0.0 0.0 0.0895755941902309 3.273662647427351E-4 52-53 4.910493971141027E-4 0.0 0.0 0.10590298664427482 3.273662647427351E-4 54-55 4.910493971141027E-4 0.0 0.0 0.1273863977680168 3.273662647427351E-4 56-57 4.910493971141027E-4 0.0 0.0 0.14608719564144557 3.273662647427351E-4 58-59 4.910493971141027E-4 0.0 0.0 0.16924835887199408 3.273662647427351E-4 60-61 4.910493971141027E-4 0.0 0.0 0.20902336003823638 3.273662647427351E-4 62-63 4.910493971141027E-4 0.0 0.0 0.23787251211868993 4.092078309284189E-4 64-65 4.910493971141027E-4 0.0 0.0 0.2714684750379131 4.092078309284189E-4 66-67 4.910493971141027E-4 8.184156618568378E-5 0.0 0.33268596654480453 4.092078309284189E-4 68-69 4.910493971141027E-4 8.184156618568378E-5 0.0 0.40818481135109785 4.092078309284189E-4 70-71 4.910493971141027E-4 8.184156618568378E-5 0.0 0.4688294118946895 4.501286140212608E-4 72-73 4.910493971141027E-4 8.184156618568378E-5 0.0 0.5394996042960275 6.547325294854702E-4 74-75 4.910493971141027E-4 8.184156618568378E-5 0.0 0.60202656086189 6.547325294854702E-4 76-77 4.910493971141027E-4 8.184156618568378E-5 0.0 0.6490445406355652 6.547325294854702E-4 78-79 4.910493971141027E-4 8.184156618568378E-5 0.0 0.7114896556352419 6.547325294854702E-4 80-81 6.547325294854702E-4 8.184156618568378E-5 0.0 0.8138325341504395 6.547325294854702E-4 82-83 6.547325294854702E-4 8.184156618568378E-5 0.0 0.9281652021118397 6.547325294854702E-4 84-85 6.547325294854702E-4 8.184156618568378E-5 0.0 1.055387916747485 6.547325294854702E-4 86-87 6.547325294854702E-4 8.184156618568378E-5 0.0 1.2477155972838423 6.547325294854702E-4 88-89 6.547325294854702E-4 8.184156618568378E-5 0.0 1.4919717515650153 6.547325294854702E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8045 0.0 27.87707 1 GGTATCA 5740 0.0 27.813686 1 GTACATG 5550 0.0 24.314007 1 ATCAACG 9315 0.0 23.873272 3 GAGTACT 4500 0.0 23.75412 12-13 TCAACGC 9385 0.0 23.644577 4 CAACGCA 9420 0.0 23.506285 5 TATCAAC 9510 0.0 23.383759 2 TACATGG 5590 0.0 23.205975 2 AACGCAG 9610 0.0 23.139482 6 ACATGGG 5545 0.0 22.62306 3 GTGTAGC 680 0.0 21.661272 1 GTACTTT 4960 0.0 21.596355 14-15 ACGCAGA 10510 0.0 20.750252 7 AGTACTT 4780 0.0 20.573652 12-13 CGCAGAG 10625 0.0 20.436224 8 AGAGTAC 7685 0.0 19.844446 10-11 CATGGGA 3185 0.0 19.842262 4 GCAGAGT 11015 0.0 18.80682 9 CATGGGG 2495 0.0 18.473541 4 >>END_MODULE