FastQCFastQC Report
Thu 26 May 2016
SRR936873_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936873_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1221873
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60060.4915404465112168No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50830.4160006809218307No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32910.26934059431708535No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23570.19290057149965667No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13780.11277767820387224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57800.027.5319841
GTATCAA80150.027.2038061
GTACATG52050.022.3596951
ATCAACG97450.022.2714373
CAACGCA98150.022.118035
TCAACGC98300.022.0788564
TATCAAC98400.022.0573222
AACGCAG100550.021.6373446
GAGTACT52850.021.47666412-13
TACATGG53300.021.2071082
AGTACTT55400.020.7452912-13
AGAGTAC83500.020.33808710-11
ACATGGG54050.020.2090573
GTACTTT56450.020.06496214-15
GTACACG1951.0321804E-519.4883881
ACGCAGA112500.019.1700787
CGCAGAG112900.018.9759338
TACTTTT63700.018.11676814-15
TCCGCGA1203.094785E-617.81141974-75
GCAGAGT118850.017.7061889
Produced by FastQC (version 0.11.5)