##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936873_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1221873 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15588363111387 33.0 31.0 34.0 31.0 34.0 2 32.284188291254495 33.0 31.0 34.0 30.0 34.0 3 32.39038427070571 34.0 31.0 34.0 31.0 34.0 4 36.04685429664131 37.0 35.0 37.0 35.0 37.0 5 35.9495495849405 37.0 35.0 37.0 35.0 37.0 6 35.99380295660842 37.0 35.0 37.0 35.0 37.0 7 35.94164368964696 37.0 35.0 37.0 35.0 37.0 8 35.908390642890055 37.0 35.0 37.0 35.0 37.0 9 37.58346571206664 39.0 37.0 39.0 35.0 39.0 10-11 37.49577697518482 39.0 37.0 39.0 35.0 39.0 12-13 37.39588197791423 39.0 37.0 39.0 34.0 39.0 14-15 38.58364699113574 40.0 38.0 41.0 34.0 41.0 16-17 38.49478382777916 40.0 38.0 41.0 34.0 41.0 18-19 38.66685899434721 40.0 38.0 41.0 34.0 41.0 20-21 38.70474591058154 40.0 38.0 41.0 34.0 41.0 22-23 38.6266968825729 40.0 38.0 41.0 34.0 41.0 24-25 38.51514437261483 40.0 38.0 41.0 34.0 41.0 26-27 38.3378419852145 40.0 38.0 41.0 34.0 41.0 28-29 38.25836441266809 40.0 38.0 41.0 34.0 41.0 30-31 38.246218305830475 40.0 38.0 41.0 34.0 41.0 32-33 38.07593260510708 40.0 37.0 41.0 33.5 41.0 34-35 37.922940845734374 40.0 37.0 41.0 33.0 41.0 36-37 37.762288306558865 40.0 37.0 41.0 33.0 41.0 38-39 37.61287711570679 40.0 37.0 41.0 33.0 41.0 40-41 37.432904238001825 40.0 37.0 41.0 32.5 41.0 42-43 37.17576294754038 40.0 36.0 41.0 31.5 41.0 44-45 36.905752070796225 39.5 36.0 41.0 31.0 41.0 46-47 36.549139313169206 39.0 36.0 41.0 30.5 41.0 48-49 36.1777386029481 39.0 35.5 40.5 30.0 41.0 50-51 35.890811483681205 39.0 35.0 40.0 29.0 41.0 52-53 35.6508086355947 39.0 35.0 40.0 28.0 41.0 54-55 35.36631507529833 38.0 35.0 40.0 27.5 41.0 56-57 35.07212697227944 38.0 34.0 40.0 27.0 41.0 58-59 34.72236271691084 38.0 34.0 40.0 26.0 41.0 60-61 34.38294241709245 37.5 34.0 40.0 26.0 41.0 62-63 34.00822016690769 37.0 33.0 40.0 25.5 41.0 64-65 33.617097685274985 36.5 33.0 39.0 24.5 40.5 66-67 33.213609352199455 36.0 32.0 39.0 23.0 40.0 68-69 32.89518632460165 35.5 32.0 39.0 22.5 40.0 70-71 32.41748856059509 35.0 31.5 38.0 22.0 40.0 72-73 31.900405770485147 35.0 31.0 37.5 20.0 39.5 74-75 31.369820349578067 34.5 31.0 37.0 18.5 39.0 76-77 29.972795044984217 33.5 29.5 35.5 18.0 37.5 78-79 30.728596179799375 34.0 30.0 36.0 18.0 38.0 80-81 30.495964801579213 34.0 30.0 35.5 16.0 37.0 82-83 30.138590508178837 34.0 30.0 35.0 12.0 37.0 84-85 29.747746697078995 34.0 29.5 35.0 7.5 36.5 86-87 29.375011969329055 34.0 29.0 35.0 6.5 36.0 88-89 28.964454980182065 34.0 29.0 35.0 2.0 35.5 90-91 28.460544999357545 33.5 29.0 35.0 2.0 35.0 92-93 27.940169313832126 33.0 27.5 35.0 2.0 35.0 94-95 27.304106482424935 33.0 26.5 34.5 2.0 35.0 96-97 26.594746753549675 32.0 25.0 34.0 2.0 35.0 98-99 25.695817814126347 32.0 24.0 34.0 2.0 35.0 100-101 22.015518388572296 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20307109395386647 1101 2 0.16212038754764535 1101 3 0.1271816586107306 1101 4 0.16596396524080603 1101 5 0.21650297136329755 1101 6 0.20347826722003504 1101 7 0.23495963121398944 1101 8 0.22724378867946626 1101 9 0.2467088526277692 1101 10-11 0.29153096253570965 1101 12-13 0.21765564546267768 1101 14-15 0.364105885393883 1101 16-17 0.43228443175396336 1101 18-19 0.3704150820737411 1101 20-21 0.32996166674636385 1101 22-23 0.31770545948263873 1101 24-25 0.4145260662329946 1101 26-27 0.35974902087681215 1101 28-29 0.3739609906044947 1101 30-31 0.42222538312528 1101 32-33 0.4641455037917268 1101 34-35 0.48513368735610385 1101 36-37 0.4022360743836586 1101 38-39 0.36987186085718804 1101 40-41 0.3929280250053395 1101 42-43 0.41899080451608484 1101 44-45 0.43341484236139394 1101 46-47 0.5067459995945356 1101 48-49 0.5722424208569876 1101 50-51 0.5353445935897909 1101 52-53 0.40446820609691514 1101 54-55 0.553957200564021 1101 56-57 0.648425084847851 1101 58-59 0.6220549471622903 1101 60-61 0.859778032617676 1101 62-63 0.7373814963846215 1101 64-65 0.5253345016170243 1101 66-67 0.36298749235852057 1101 68-69 0.4957760232291406 1101 70-71 0.4718494862687024 1101 72-73 0.6028157640885539 1101 74-75 0.5248318633543647 1101 76-77 0.27759387668909596 1101 78-79 0.4049034050185156 1101 80-81 0.6065288613879893 1101 82-83 0.5866452730951863 1101 84-85 0.5768966312140051 1101 86-87 0.22256978924983883 1101 88-89 0.2606773280662047 1101 90-91 0.5198709601027751 1101 92-93 0.5324713506990619 1101 94-95 0.4690652694378805 1101 96-97 0.7942777434086672 1101 98-99 0.9319144203809593 1101 100-101 1.3541098488705074 1102 1 0.2866183149306707 1102 2 0.3550719503894584 1102 3 0.33826367378249245 1102 4 0.20146895306455548 1102 5 0.24818000210463964 1102 6 0.23488455410888065 1102 7 0.22351763501595912 1102 8 0.3517491396609671 1102 9 0.30409138769992694 1102 10-11 0.3438744130253255 1102 12-13 0.41852520251851644 1102 14-15 0.5328446450116076 1102 16-17 0.45798416021592914 1102 18-19 0.3930651517812578 1102 20-21 0.39461529872099277 1102 22-23 0.375683270038472 1102 24-25 0.5238286084094739 1102 26-27 0.4162640926455907 1102 28-29 0.3796119562936724 1102 30-31 0.5300882899901183 1102 32-33 0.480619364736242 1102 34-35 0.48348720374116283 1102 36-37 0.5410785758258214 1102 38-39 0.4380682663895996 1102 40-41 0.2015316148522288 1102 42-43 0.38955697684058066 1102 44-45 0.34548445890867896 1102 46-47 0.31541312399880894 1102 48-49 0.28292265215164747 1102 50-51 0.18663084348420256 1102 52-53 0.2625673820970249 1102 54-55 0.22568425032962836 1102 56-57 0.01585806491461028 1102 58-59 0.23961625981512924 1102 60-61 0.17772770025252527 1102 62-63 0.4193195602989377 1102 64-65 0.41953971679341606 1102 66-67 0.040060108747688616 1102 68-69 0.0619761440891935 1102 70-71 -0.018926451172859515 1102 72-73 0.37519202151325715 1102 74-75 0.43057004466322013 1102 76-77 0.3521090827468285 1102 78-79 0.3131818856473352 1102 80-81 0.35535040364604953 1102 82-83 0.32769466182776696 1102 84-85 0.4288798840822672 1102 86-87 0.4233122771909059 1102 88-89 0.3015827532676205 1102 90-91 0.505071740133868 1102 92-93 0.4333267568762089 1102 94-95 0.3844004178379983 1102 96-97 0.20269881579980265 1102 98-99 0.3467550100105292 1102 100-101 0.46303623758792156 1103 1 0.28646811691852747 1103 2 0.2728493608707865 1103 3 0.24599618227293973 1103 4 0.14617771977490435 1103 5 0.11239771969827217 1103 6 0.20043936195381207 1103 7 0.1694988215671387 1103 8 0.13230984392567535 1103 9 0.253714712073851 1103 10-11 0.273060969450583 1103 12-13 0.320320298323864 1103 14-15 0.3547950678616445 1103 16-17 0.3197842801119819 1103 18-19 0.29015098240250126 1103 20-21 0.29539639397277284 1103 22-23 0.36316469205291213 1103 24-25 0.3646570849031576 1103 26-27 0.3357107343098633 1103 28-29 0.2768797954230706 1103 30-31 0.2732037771760645 1103 32-33 0.38687481679290414 1103 34-35 0.43409402994566193 1103 36-37 0.3379944662507981 1103 38-39 0.2668281030476223 1103 40-41 0.4060025964651075 1103 42-43 0.5180615424666115 1103 44-45 0.5192078085998233 1103 46-47 0.4627213001489423 1103 48-49 0.6056309518708005 1103 50-51 0.7385964041950288 1103 52-53 0.6557480644026441 1103 54-55 0.7274052965473317 1103 56-57 0.8818748792213285 1103 58-59 0.7258872432644878 1103 60-61 0.7354194781903374 1103 62-63 0.8059358024102679 1103 64-65 0.655042985075923 1103 66-67 0.5794260894882441 1103 68-69 0.5645426280258832 1103 70-71 0.4104132547538626 1103 72-73 0.3514843475098388 1103 74-75 0.6063151645396019 1103 76-77 0.525537611449078 1103 78-79 0.691934376376377 1103 80-81 0.8494562794709921 1103 82-83 0.7625772647002869 1103 84-85 0.6706386700100886 1103 86-87 0.5235487898685385 1103 88-89 0.5099100196506825 1103 90-91 0.7278206392283515 1103 92-93 0.8214433393926335 1103 94-95 0.810942111887929 1103 96-97 0.7083725621055201 1103 98-99 0.5508836188621125 1103 100-101 0.5327350020289074 1104 1 0.24813061812303516 1104 2 0.2574859410690493 1104 3 0.23187142332695743 1104 4 0.06750354429856031 1104 5 0.09112458857586603 1104 6 0.08741872492473846 1104 7 0.13710945636405114 1104 8 0.18669565476368177 1104 9 0.31202935266703946 1104 10-11 0.21240439991298388 1104 12-13 0.1897367189050314 1104 14-15 0.09862097471873454 1104 16-17 0.27222238671122767 1104 18-19 0.2844989442534498 1104 20-21 0.26113074116617696 1104 22-23 0.21003394777081752 1104 24-25 0.17340534827745557 1104 26-27 0.044339400739900725 1104 28-29 0.004720817479899608 1104 30-31 0.0619129962037448 1104 32-33 0.09973359237878299 1104 34-35 0.2140249796344449 1104 36-37 0.2795249168159657 1104 38-39 0.4356287304894195 1104 40-41 0.37351595582800456 1104 42-43 0.3676468868704461 1104 44-45 0.24903459124076477 1104 46-47 0.5152286888127833 1104 48-49 0.28260546021819266 1104 50-51 0.3697685152366077 1104 52-53 0.3731184075318268 1104 54-55 0.2648200509436336 1104 56-57 0.4245211613481388 1104 58-59 0.45215478181179236 1104 60-61 0.6721636114753693 1104 62-63 0.6222547780115164 1104 64-65 0.4376735567627321 1104 66-67 0.2570641443698847 1104 68-69 0.2516987334468368 1104 70-71 0.18420639900082847 1104 72-73 0.32907440504567376 1104 74-75 0.1566413269352971 1104 76-77 0.20324153308228077 1104 78-79 0.19690213216197705 1104 80-81 0.5238851598459924 1104 82-83 0.45623252788065116 1104 84-85 0.1646975500069665 1104 86-87 0.33878340357833636 1104 88-89 0.3832323995871789 1104 90-91 0.19119872536010618 1104 92-93 0.3721756800896294 1104 94-95 0.06754952287328209 1104 96-97 0.017242800945677317 1104 98-99 0.07935665679526593 1104 100-101 0.17035595595023523 1105 1 0.2642935555085586 1105 2 0.24864921494025793 1105 3 0.1780979503908071 1105 4 0.13263860965631835 1105 5 0.2209022265931182 1105 6 0.18615708710815682 1105 7 0.1612134561389098 1105 8 0.14265395990359764 1105 9 0.1440960722741238 1105 10-11 0.2185538289273481 1105 12-13 0.1376915588540797 1105 14-15 0.1501146041268484 1105 16-17 0.32348720584710833 1105 18-19 0.16440628221111808 1105 20-21 0.27339503293720213 1105 22-23 0.2913227736315136 1105 24-25 0.2539826598424071 1105 26-27 0.25891602220328025 1105 28-29 0.25030520748027385 1105 30-31 0.18220602018233478 1105 32-33 0.1038463652533892 1105 34-35 0.34930507644443765 1105 36-37 0.27591687248305874 1105 38-39 0.44150866505213315 1105 40-41 0.14920847397682024 1105 42-43 0.0021946684508478143 1105 44-45 -0.006533329314358127 1105 46-47 0.23007327524739196 1105 48-49 0.14656765880348388 1105 50-51 0.03692053702619802 1105 52-53 0.12104718513436552 1105 54-55 0.18179893554862048 1105 56-57 0.25575859666641065 1105 58-59 0.14602774439090638 1105 60-61 0.12044759561282348 1105 62-63 0.1986596669651206 1105 64-65 -0.10075922746673172 1105 66-67 -0.19076833714481722 1105 68-69 -0.14471720447338754 1105 70-71 -0.25922668500680146 1105 72-73 -0.2332867946053625 1105 74-75 -0.19945781276255303 1105 76-77 -0.143610979731708 1105 78-79 0.1355773084498928 1105 80-81 0.07462597898676648 1105 82-83 0.03936866020395513 1105 84-85 0.09347851739721946 1105 86-87 -0.08622892403392157 1105 88-89 -0.29776509084133096 1105 90-91 -0.20444880159240242 1105 92-93 -0.17688607543577106 1105 94-95 -0.37656326223716263 1105 96-97 -0.03544565857386672 1105 98-99 -0.251176605733324 1105 100-101 -0.06432476806909193 1106 1 0.21765068493766648 1106 2 0.22670781486066716 1106 3 0.07803715803941458 1106 4 0.1294573874126641 1106 5 0.14391445098281253 1106 6 0.1610857354427111 1106 7 0.1470346777966185 1106 8 0.17480066234578828 1106 9 0.2850586422524657 1106 10-11 0.19326640806116302 1106 12-13 0.1487324508658645 1106 14-15 0.288329921357942 1106 16-17 0.2673464459182995 1106 18-19 0.04162648480346576 1106 20-21 0.24800873294526582 1106 22-23 0.18357931735439337 1106 24-25 0.25967919614325297 1106 26-27 0.19315468955681325 1106 28-29 0.10013955442637013 1106 30-31 0.14673588045757668 1106 32-33 0.28474145328022615 1106 34-35 0.07292937482989714 1106 36-37 0.23120885887924914 1106 38-39 0.2278809242082147 1106 40-41 0.2973800646611906 1106 42-43 -0.04459102752392141 1106 44-45 -0.04487584660599708 1106 46-47 0.06442205244220389 1106 48-49 0.12327735213170854 1106 50-51 0.11563381441754927 1106 52-53 0.06937002425924987 1106 54-55 -0.18339951851897496 1106 56-57 -0.15790405705400445 1106 58-59 -0.10538647125347467 1106 60-61 -0.051945718693403364 1106 62-63 0.26148758554730733 1106 64-65 -0.11923070267005187 1106 66-67 -0.06796473550317472 1106 68-69 -0.12465328705332723 1106 70-71 -0.1119486686441391 1106 72-73 -0.13802041912348173 1106 74-75 -0.09111131589128263 1106 76-77 -0.06311665734669347 1106 78-79 0.10297466337224392 1106 80-81 0.1502086944272918 1106 82-83 0.1212363037801083 1106 84-85 -0.00618553772117636 1106 86-87 0.031102145252265956 1106 88-89 -0.13748019688614121 1106 90-91 0.010031008754996407 1106 92-93 0.12295449723306007 1106 94-95 -0.1095371312919049 1106 96-97 0.028550498568257865 1106 98-99 -0.41828476973106987 1106 100-101 -0.07083553124504505 1107 1 0.13279761450716165 1107 2 0.16621650281264522 1107 3 0.32088912771222056 1107 4 0.15653386848934048 1107 5 0.18247094755164284 1107 6 0.17282115549928534 1107 7 0.1563059833935725 1107 8 0.13011445623070728 1107 9 0.24223221303610387 1107 10-11 0.23641868793660592 1107 12-13 0.22745431289703788 1107 14-15 0.26246571265044594 1107 16-17 0.13221045198086046 1107 18-19 0.23690815526921938 1107 20-21 0.1397612639085679 1107 22-23 0.11191285653021765 1107 24-25 0.228474573920181 1107 26-27 0.23420587718803887 1107 28-29 0.23279044996110798 1107 30-31 0.2101142833037244 1107 32-33 0.25069260745705435 1107 34-35 0.2640673160772522 1107 36-37 0.2844382277661808 1107 38-39 0.28931891186006453 1107 40-41 0.25882806465159547 1107 42-43 0.45427464046693444 1107 44-45 0.32097920111149136 1107 46-47 0.2919383569659786 1107 48-49 0.23324970129012712 1107 50-51 0.29165462329459757 1107 52-53 0.3622764922299595 1107 54-55 0.42564114356639493 1107 56-57 0.28535805877496045 1107 58-59 0.2614653137606311 1107 60-61 0.3028846650969186 1107 62-63 0.5030688275809112 1107 64-65 0.3487492990326473 1107 66-67 0.27330162747586684 1107 68-69 0.38563813794826274 1107 70-71 0.4223262042231468 1107 72-73 0.45498671181992734 1107 74-75 0.43843557482793827 1107 76-77 0.407941389036214 1107 78-79 0.5927977507457847 1107 80-81 0.5653472304195546 1107 82-83 0.7361677582311614 1107 84-85 0.6898635455203248 1107 86-87 0.47049931853527127 1107 88-89 0.5233224569231645 1107 90-91 0.583837232232792 1107 92-93 0.618775994623256 1107 94-95 0.799811635032107 1107 96-97 1.0055706827233415 1107 98-99 1.0014753298407406 1107 100-101 1.0229872755432012 1108 1 0.09944644349901921 1108 2 0.020550177643571033 1108 3 0.054611956063325806 1108 4 0.004046393001480908 1108 5 0.08567574400851186 1108 6 0.12339260653545381 1108 7 0.08121876068433664 1108 8 0.02606506934210273 1108 9 0.08163504993051873 1108 10-11 0.14595204497526737 1108 12-13 0.16660730499095422 1108 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-0.5649837395191142 2316 62-63 -0.6006099257176558 2316 64-65 -0.6138257161534142 2316 66-67 -0.632575460731239 2316 68-69 -0.8842546131724731 2316 70-71 -0.6006474541435693 2316 72-73 -0.33817121728277044 2316 74-75 -0.616090794836083 2316 76-77 -0.5795710846396318 2316 78-79 -0.6473685737261015 2316 80-81 -0.690980954712554 2316 82-83 -0.6412050432525547 2316 84-85 -0.8962821496517286 2316 86-87 -0.7572236692373941 2316 88-89 -1.1345749875314652 2316 90-91 -0.940277771595877 2316 92-93 -1.1792009120106606 2316 94-95 -1.0869666313231683 2316 96-97 -0.4426242370527014 2316 98-99 -0.4037560622049945 2316 100-101 -0.6743441327933368 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 9.0 5 37.0 6 58.0 7 110.0 8 283.0 9 619.0 10 1050.0 11 1842.0 12 2669.0 13 3818.0 14 4913.0 15 6888.0 16 9057.0 17 12451.0 18 11448.0 19 7152.0 20 6295.0 21 6451.0 22 6861.0 23 7833.0 24 8873.0 25 10830.0 26 13387.0 27 16306.0 28 20159.0 29 25381.0 30 31492.0 31 39642.0 32 49949.0 33 65089.0 34 87780.0 35 123491.0 36 186002.0 37 268300.0 38 175323.0 39 10023.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.519403356508484 19.952650980257324 15.462434725234065 22.065510938000127 2 15.456356481542107 21.281248772311198 39.60229430483349 23.6601004413132 3 19.48601775473843 25.2508652713748 30.4234244040492 24.839692569837567 4 12.943008760328414 17.694979506855056 37.54975971296503 31.812252019851506 5 13.421362121922655 37.86072693315917 34.213457536094175 14.504453408823995 6 30.905339589302656 37.071365027298256 17.523097736016755 14.500197647382338 7 27.558837947970044 33.44250998262503 21.146633078887906 17.852018990517017 8 26.1802167655722 34.70761691272334 19.91729091321275 19.19487540849172 9 26.024493563973966 16.997606950646222 20.84170846046067 36.13619102491914 10-11 24.466263935537917 27.14719112021651 27.549117386943145 20.837427557302423 12-13 25.75860993736665 24.670812760409632 28.086143158904402 21.484434143319316 14-15 22.62477360576754 25.735080487088265 26.92047373172171 24.719672175422485 16-17 21.49261829993788 29.139198591015596 27.402397794206106 21.965785314840414 18-19 21.32091739672903 27.43564068368947 30.25361525628125 20.989826663300242 20-21 22.23997289385144 26.327215080577776 30.35152058782368 21.08129143774711 22-23 21.933305779374635 26.41689412323167 30.32176749096653 21.328032606427165 24-25 21.88486817873016 26.508230132680705 30.39628232097108 21.21061936761805 26-27 21.659099326203187 26.689556251700864 30.337307636432087 21.314036785663863 28-29 21.34447309817992 26.865768752624053 30.511822462146103 21.27793568704992 30-31 21.616539584210592 26.49052313940113 30.40174097440386 21.491196301984417 32-33 21.467304677413214 26.721808517386176 30.257235424083035 21.55365138111757 34-35 22.025816103637613 26.605792909737758 30.092407312380253 21.27598367424438 36-37 21.94790360911296 26.386970541135717 30.154748764993293 21.510377084758026 38-39 21.64384649258508 26.75640855641936 30.212666342535073 21.387078608460488 40-41 21.8574550275809 26.631160689440687 30.15721932136251 21.354164961615897 42-43 21.64247020762387 26.798611639671226 29.75309217897441 21.805825973730496 44-45 21.88021995739328 26.800166629428755 29.529705624070584 21.789907789107378 46-47 22.156262250699545 27.054127733824167 29.1181378691169 21.67147214635939 48-49 22.149245033936438 27.247936109631105 28.805010136326842 21.79780872010561 50-51 21.927197577344902 27.41856277623179 28.776886560811914 21.877353085611393 52-53 22.282839542243753 27.57275101422161 28.433642448928815 21.710766994605823 54-55 22.069737673468452 27.31250347837189 28.599173702526357 22.018585145633292 56-57 22.194790882755782 27.62218574713434 28.35959907958376 21.823424290526123 58-59 22.37496624027106 27.361012218975834 28.398029250247568 21.865992290505538 60-61 22.166460013667745 27.392120996353903 28.847735615109816 21.59368337486854 62-63 22.022316589139294 27.428362373872233 28.826515057579776 21.722805979408697 64-65 21.94156021124945 27.474336530883324 28.634686256263947 21.949417001603276 66-67 21.93848091641476 27.612875082303574 28.37610391140964 22.072540089872025 68-69 21.913373621373175 27.457016633547976 28.626046348705426 22.003563396373423 70-71 22.06099716825168 27.364100633460463 28.52855482624851 22.04634737203935 72-73 22.08064995298202 27.33680177890828 28.536271772925666 22.046276495184035 74-75 21.974419600073002 27.84532435040303 28.07787716071965 22.10237888880432 76-77 22.06080337318199 27.569354589224904 28.198307025361885 22.17153501223122 78-79 22.048501063347 27.648822543201923 28.1725367976989 22.130139595752176 80-81 22.182146446541807 27.766578930999824 28.008424915619894 22.04284970683847 82-83 22.159411565136715 27.64220488733574 28.24130478465512 21.957078762872424 84-85 22.030527316046687 27.71631973062496 28.081766848830934 22.171386104497415 86-87 22.065493884552932 27.522949295479542 28.175129969619196 22.23642685034833 88-89 22.05071398640774 27.55333619233439 27.979362813780824 22.41658700747705 90-91 21.974254090471607 27.590427412543455 28.147486135936568 22.287832361048366 92-93 22.088119885143218 27.631428724589657 28.018090311608564 22.262361078658564 94-95 21.90707295564799 27.36222478204559 28.10698333909635 22.623718923210074 96-97 21.781215549195444 27.396507430120366 28.297394690514636 22.524882330169554 98-99 21.744242051733657 27.46419479569785 27.922268977198183 22.869294175370307 100-101 21.609190510603018 27.618386259083422 27.891942589590208 22.88048064072335 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 393.0 1 294.0 2 228.5 3 286.0 4 255.0 5 225.0 6 416.5 7 920.5 8 1537.5 9 1892.5 10 1742.5 11 1480.0 12 1440.0 13 1484.0 14 1644.0 15 1819.5 16 1935.5 17 2058.5 18 2186.0 19 2330.0 20 2565.0 21 2979.5 22 3527.0 23 4133.5 24 4947.5 25 6106.0 26 7654.0 27 9620.5 28 11310.5 29 13063.0 30 15514.0 31 17933.5 32 20275.0 33 22794.5 34 25646.0 35 27890.5 36 31075.5 37 34524.0 38 36179.0 39 37331.0 40 38346.5 41 39788.5 42 41182.5 43 42482.0 44 43705.0 45 44804.5 46 44953.5 47 46165.5 48 50817.5 49 50831.5 50 45779.5 51 42520.0 52 40141.0 53 38356.5 54 36702.5 55 33758.0 56 30495.5 57 27291.5 58 24489.0 59 22247.0 60 19201.5 61 15090.0 62 11526.5 63 8762.0 64 6435.0 65 4862.5 66 3562.5 67 2583.0 68 1761.0 69 1134.0 70 779.0 71 564.5 72 415.5 73 290.5 74 228.5 75 168.0 76 102.5 77 46.5 78 22.5 79 16.0 80 7.0 81 4.5 82 3.5 83 2.5 84 2.0 85 0.0 86 1.5 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025043519252819234 2 0.005319701802069446 3 1.6368313237136755E-4 4 8.184156618568378E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 8.184156618568378E-5 10-11 0.010762165953417417 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.0460391546420946E-4 20-21 8.184156618568379E-4 22-23 0.002291563853199146 24-25 0.011703343964552781 26-27 0.005360622585162288 28-29 3.273662647427351E-4 30-31 0.002332484636291988 32-33 0.004460365357119766 34-35 0.0 36-37 0.009698225593003528 38-39 0.012030710229295517 40-41 0.0010639403604138893 42-43 0.0 44-45 0.0 46-47 3.273662647427351E-4 48-49 0.0024552469855705136 50-51 0.005974434331554916 52-53 0.0 54-55 0.0030281379488703 56-57 0.0028235340334060903 58-59 2.4552469855705135E-4 60-61 0.001473148191342308 62-63 0.003805632827634296 64-65 0.0 66-67 0.002005118371549253 68-69 4.092078309284189E-4 70-71 0.0010639403604138893 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0019232768053635686 80-81 0.0017186728898993594 82-83 0.010107433423931946 84-85 0.006711008427226071 86-87 0.004010236743098506 88-89 8.184156618568378E-5 90-91 7.36574095671154E-4 92-93 2.864454816498932E-4 94-95 4.092078309284189E-5 96-97 0.0018005144560850435 98-99 0.00151406897443515 100-101 3.273662647427351E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1221873.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.83320961345929 #Duplication Level Percentage of deduplicated Percentage of total 1 82.92349546899254 53.76196363621935 2 10.572947880750403 13.709562923697419 3 2.8891511507688086 5.619388264882839 4 1.1719119678140062 3.03915257031228 5 0.6080810695971461 1.9711923723584153 6 0.36940680008902727 1.4369897101685492 7 0.25010034924834396 1.1350365857052078 8 0.19402736301174156 1.0063533357509598 9 0.13568186000132496 0.7917021423188944 >10 0.7934689932873233 9.525521647952047 >50 0.06312851521228217 2.824033848879747 >100 0.026573910979009654 3.0755673855474384 >500 0.001265418905032948 0.5228483707943371 >1k 5.061675620131791E-4 0.6709326810538346 >5k 2.5308378100658956E-4 0.909754524358764 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6006 0.4915404465112168 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5083 0.4160006809218307 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3291 0.26934059431708535 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2357 0.19290057149965667 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1378 0.11277767820387224 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 8.184156618568378E-5 0.0 14-15 0.0 0.0 0.0 8.184156618568378E-5 0.0 16-17 0.0 0.0 0.0 8.184156618568378E-5 0.0 18-19 0.0 0.0 0.0 8.184156618568378E-5 0.0 20-21 0.0 0.0 0.0 1.6368313237136755E-4 0.0 22-23 0.0 0.0 0.0 3.68287047835577E-4 0.0 24-25 0.0 0.0 0.0 4.910493971141027E-4 0.0 26-27 0.0 0.0 0.0 0.0010639403604138893 0.0 28-29 0.0 0.0 0.0 0.0018005144560850432 0.0 30-31 0.0 0.0 0.0 0.002905375599591774 0.0 32-33 0.0 0.0 0.0 0.004869573188048185 0.0 34-35 0.0 0.0 0.0 0.006342721379390494 0.0 36-37 0.0 0.0 0.0 0.009043493063518059 0.0 38-39 0.0 0.0 0.0 0.011007690651974468 0.0 40-41 0.0 0.0 0.0 0.013626620769916348 0.0 42-43 0.0 8.184156618568378E-5 0.0 0.017636857513014855 0.0 44-45 0.0 8.184156618568378E-5 0.0 0.02463431142189082 0.0 46-47 0.0 8.184156618568378E-5 0.0 0.0385882984565499 0.0 48-49 0.0 8.184156618568378E-5 0.0 0.0696471728240169 0.0 50-51 0.0 8.184156618568378E-5 0.0 0.08961651497332374 0.0 52-53 0.0 1.6368313237136755E-4 0.0 0.1056983827288106 0.0 54-55 0.0 1.6368313237136755E-4 0.0 0.12726363541873828 0.0 56-57 0.0 1.6368313237136755E-4 0.0 0.14584167094288852 0.0 58-59 0.0 1.6368313237136755E-4 0.0 0.1691665173058084 0.0 60-61 0.0 2.4552469855705135E-4 0.0 0.20890059768895786 8.184156618568378E-5 62-63 0.0 2.4552469855705135E-4 0.0 0.23791343290178274 8.184156618568378E-5 64-65 0.0 2.4552469855705135E-4 0.0 0.27093650485770615 8.184156618568378E-5 66-67 0.0 2.4552469855705135E-4 0.0 0.3317857093167621 8.184156618568378E-5 68-69 0.0 2.4552469855705135E-4 0.0 0.4069162670752198 8.184156618568378E-5 70-71 0.0 2.4552469855705135E-4 0.0 0.4672744221371615 8.184156618568378E-5 72-73 0.0 2.4552469855705135E-4 0.0 0.5374944859244782 8.184156618568378E-5 74-75 0.0 2.4552469855705135E-4 0.0 0.5996122346594122 8.184156618568378E-5 76-77 0.0 2.4552469855705135E-4 0.0 0.6464665313007162 8.184156618568378E-5 78-79 0.0 2.4552469855705135E-4 0.0 0.7082569137709074 8.184156618568378E-5 80-81 0.0 2.4552469855705135E-4 0.0 0.8101496636720837 8.184156618568378E-5 82-83 0.0 2.4552469855705135E-4 0.0 0.9250552225967839 1.6368313237136755E-4 84-85 0.0 2.4552469855705135E-4 0.0 1.0535464815083073 1.6368313237136755E-4 86-87 0.0 2.4552469855705135E-4 0.0 1.2453012710813645 1.6368313237136755E-4 88-89 0.0 2.4552469855705135E-4 0.0 1.487961514821917 1.6368313237136755E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5780 0.0 27.531984 1 GTATCAA 8015 0.0 27.203806 1 GTACATG 5205 0.0 22.359695 1 ATCAACG 9745 0.0 22.271437 3 CAACGCA 9815 0.0 22.11803 5 TCAACGC 9830 0.0 22.078856 4 TATCAAC 9840 0.0 22.057322 2 AACGCAG 10055 0.0 21.637344 6 GAGTACT 5285 0.0 21.476664 12-13 TACATGG 5330 0.0 21.207108 2 AGTACTT 5540 0.0 20.74529 12-13 AGAGTAC 8350 0.0 20.338087 10-11 ACATGGG 5405 0.0 20.209057 3 GTACTTT 5645 0.0 20.064962 14-15 GTACACG 195 1.0321804E-5 19.488388 1 ACGCAGA 11250 0.0 19.170078 7 CGCAGAG 11290 0.0 18.975933 8 TACTTTT 6370 0.0 18.116768 14-15 TCCGCGA 120 3.094785E-6 17.811419 74-75 GCAGAGT 11885 0.0 17.706188 9 >>END_MODULE