FastQCFastQC Report
Thu 26 May 2016
SRR936872_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936872_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214393
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57300.4718406644307073No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42410.3492279682112792No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26760.22035700139905284No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20840.17160836730778256No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13070.10762578506299032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA78650.030.3460831
GGTATCA54450.027.7668671
ATCAACG92300.025.65333
TCAACGC92650.025.5574444
CAACGCA93200.025.4565875
TATCAAC93500.025.374912
AACGCAG96050.024.6981836
GAGTACT45200.023.98000512-13
GTGTTCG1401.7702061E-523.7662249
GTACATG55850.022.3036421
ACGCAGA106000.022.24167
CGCAGAG106150.021.9871838
GTACTTT49450.021.9666514-15
TACATGG56450.020.9725382
AGAGTAC78600.020.7727710-11
ACATGGG54550.020.6570913
GCAGAGT109700.020.6248389
AGTACTT48300.020.47241412-13
GTATAGG3353.45608E-1119.8692231
GTGTAGC5750.019.844541
Produced by FastQC (version 0.11.5)