##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936872_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1214393 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.636661278515273 33.0 31.0 34.0 30.0 34.0 2 31.716492931036328 33.0 31.0 34.0 30.0 34.0 3 31.734072907205494 33.0 31.0 34.0 30.0 34.0 4 35.33580397778973 37.0 35.0 37.0 33.0 37.0 5 35.33770451575396 37.0 35.0 37.0 33.0 37.0 6 35.40018428959983 37.0 35.0 37.0 33.0 37.0 7 35.341656284250654 37.0 35.0 37.0 33.0 37.0 8 35.30646833438598 37.0 35.0 37.0 33.0 37.0 9 36.90879723450316 39.0 37.0 39.0 33.0 39.0 10-11 36.775007349350666 39.0 37.0 39.0 32.5 39.0 12-13 36.68375599991107 39.0 37.0 39.0 32.0 39.0 14-15 37.793181449497816 40.0 37.5 41.0 32.0 41.0 16-17 37.72498318089778 40.0 37.0 41.0 32.0 41.0 18-19 37.685937336595316 40.0 37.0 41.0 32.0 41.0 20-21 37.62408256635208 40.0 37.0 41.0 32.0 41.0 22-23 37.50981436816582 40.0 37.0 41.0 32.0 41.0 24-25 37.46485816370813 40.0 37.0 41.0 32.0 41.0 26-27 37.488574950613184 40.0 37.0 41.0 32.0 41.0 28-29 37.413629689894464 40.0 37.0 41.0 32.0 41.0 30-31 37.25389680276484 40.0 37.0 41.0 31.0 41.0 32-33 37.06004028349966 39.0 36.0 41.0 31.0 41.0 34-35 36.89422246340353 39.0 36.0 41.0 30.5 41.0 36-37 36.70981263890685 39.0 36.0 41.0 30.0 41.0 38-39 36.547185301628055 39.0 35.0 41.0 30.0 41.0 40-41 36.37435821846799 39.0 35.0 41.0 30.0 41.0 42-43 36.157067769659406 39.0 35.0 40.5 29.5 41.0 44-45 35.89928631011543 39.0 35.0 40.0 28.5 41.0 46-47 35.5542872035659 38.5 35.0 40.0 27.0 41.0 48-49 35.29862779182686 38.5 35.0 40.0 26.0 41.0 50-51 34.59580629993749 38.0 34.0 39.5 25.0 40.5 52-53 34.7896949340123 38.0 34.5 40.0 26.0 40.5 54-55 35.184199431320835 39.0 35.0 40.0 26.0 41.0 56-57 35.06753044525125 39.0 35.0 40.0 26.0 41.0 58-59 34.85048991553805 38.0 34.0 40.0 26.0 41.0 60-61 34.56187823875796 38.0 34.0 40.0 25.0 41.0 62-63 34.25457203722353 38.0 34.0 40.0 24.0 41.0 64-65 33.92243697056884 37.0 33.5 40.0 23.0 41.0 66-67 33.555859594052336 37.0 33.0 39.5 22.0 41.0 68-69 33.16671950513549 36.0 33.0 39.0 22.0 41.0 70-71 32.750507043436514 36.0 32.0 39.0 20.5 40.5 72-73 32.35655014480486 35.0 32.0 38.5 20.0 40.0 74-75 31.96186201666182 35.0 32.0 37.5 18.5 39.5 76-77 31.49878416624602 35.0 31.0 37.0 17.5 39.0 78-79 31.033556682227253 35.0 31.0 36.5 13.0 39.0 80-81 30.597410393505232 35.0 31.0 36.0 9.0 37.5 82-83 30.183419206138375 34.0 30.0 35.5 7.0 37.0 84-85 29.812923822848123 34.0 30.0 35.0 4.5 37.0 86-87 29.464554308201713 34.0 29.5 35.0 2.0 36.0 88-89 29.095595083304993 34.0 29.0 35.0 2.0 36.0 90-91 28.718503400464265 34.0 29.0 35.0 2.0 36.0 92-93 28.32625188056914 34.0 29.0 35.0 2.0 35.0 94-95 27.94369368071127 34.0 28.5 35.0 2.0 35.0 96-97 27.440912044124104 34.0 27.0 35.0 2.0 35.0 98-99 26.865804974172285 34.0 26.0 35.0 2.0 35.0 100-101 24.676344889998543 31.0 20.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3975537524409809 1101 2 0.22568076019043914 1101 3 0.4068743203337952 1101 4 0.45942088265348957 1101 5 0.35858377254992035 1101 6 0.3895475046036623 1101 7 0.4580454490875141 1101 8 0.4180192525133961 1101 9 0.4026240588586063 1101 10-11 0.46811586966907726 1101 12-13 0.5757107455028176 1101 14-15 0.7384591988963152 1101 16-17 0.8321794695873805 1101 18-19 0.7439111764860229 1101 20-21 0.8182279547681972 1101 22-23 0.7615436616226958 1101 24-25 0.8203735925303093 1101 26-27 0.7498538956460905 1101 28-29 0.8759778613506342 1101 30-31 0.7381649827598551 1101 32-33 0.7599881664011221 1101 34-35 0.8854841616806581 1101 36-37 1.0126712736301897 1101 38-39 0.7150909829549192 1101 40-41 1.067076248706087 1101 42-43 1.0660438876932332 1101 44-45 1.0041229133181915 1101 46-47 1.0478173448670631 1101 48-49 0.9897192535144512 1101 50-51 0.9836946844011578 1101 52-53 0.8772850333753652 1101 54-55 1.0638784363586637 1101 56-57 1.1105201889199776 1101 58-59 1.0313166213441392 1101 60-61 1.1195975329926853 1101 62-63 0.8874135680628612 1101 64-65 0.8337096797833965 1101 66-67 0.7350058503267647 1101 68-69 0.770964401214222 1101 70-71 0.6427867036270456 1101 72-73 0.6505439866644664 1101 74-75 0.7535562793397439 1101 76-77 0.8146588571950026 1101 78-79 0.7765285140757463 1101 80-81 0.9220934481871765 1101 82-83 0.7104903330831505 1101 84-85 0.8033738553102303 1101 86-87 0.8169823184944356 1101 88-89 0.9164210740681717 1101 90-91 0.8451939270459405 1101 92-93 1.0277389467509188 1101 94-95 1.0544264749614953 1101 96-97 1.1511836117366272 1101 98-99 1.0280501838952212 1101 100-101 1.0530608739711553 1102 1 0.3186368446559342 1102 2 0.2678008619529315 1102 3 0.19224327733622104 1102 4 0.17530152718552472 1102 5 0.3360304529326399 1102 6 0.29817876102244156 1102 7 0.3194426510947608 1102 8 0.3500731461249629 1102 9 0.3673952869616883 1102 10-11 0.3347714574643774 1102 12-13 0.35640992423899576 1102 14-15 0.34676536180737827 1102 16-17 0.5908140224801528 1102 18-19 0.345842910247832 1102 20-21 0.1685248753177362 1102 22-23 0.1466248398436747 1102 24-25 0.23294940293615696 1102 26-27 0.32183087606231453 1102 28-29 0.12781522080020125 1102 30-31 0.11121471057985133 1102 32-33 0.13654133240951438 1102 34-35 0.03524977218423686 1102 36-37 0.1837202116557961 1102 38-39 0.11499100123845807 1102 40-41 0.05695437432417805 1102 42-43 -0.05230496625529213 1102 44-45 0.10505143148855467 1102 46-47 0.15004848002997306 1102 48-49 0.3393827073084168 1102 50-51 0.22556037993338407 1102 52-53 0.5035644561049892 1102 54-55 0.28956897969234774 1102 56-57 0.45791009518456605 1102 58-59 0.2625659713950199 1102 60-61 0.3007428571992037 1102 62-63 0.11552583033441266 1102 64-65 0.22460964437178177 1102 66-67 -0.15036079574544203 1102 68-69 0.07662978259586595 1102 70-71 0.13440686282294934 1102 72-73 0.1439405035488619 1102 74-75 0.2729290113146554 1102 76-77 0.13720354480821229 1102 78-79 0.17526525086555012 1102 80-81 0.2619531203477372 1102 82-83 0.2623921267048459 1102 84-85 0.05453594735458012 1102 86-87 0.08214694848392057 1102 88-89 0.29833016071377827 1102 90-91 0.14417358338342723 1102 92-93 0.24536672250096814 1102 94-95 0.5260676228413175 1102 96-97 0.3756969400266925 1102 98-99 0.545271371862885 1102 100-101 0.4563355300673564 1103 1 0.38591356979972957 1103 2 0.3013249829668325 1103 3 0.25235872549044913 1103 4 0.28834804147020776 1103 5 0.04444042865951303 1103 6 0.0842954594157419 1103 7 0.22094589029219946 1103 8 0.3135858044746769 1103 9 0.30325121937176647 1103 10-11 0.3285870099763386 1103 12-13 0.23332266807733504 1103 14-15 0.3572601817870762 1103 16-17 0.5206023161862134 1103 18-19 0.4471308875432811 1103 20-21 0.38535246426891234 1103 22-23 0.38759365960001446 1103 24-25 0.41633887294517535 1103 26-27 0.44304373632435556 1103 28-29 0.5297962849410567 1103 30-31 0.5251558389527062 1103 32-33 0.36910280784565686 1103 34-35 0.437725027334416 1103 36-37 0.39256501129022325 1103 38-39 0.543820115121882 1103 40-41 0.6065223743652481 1103 42-43 0.6537367434658847 1103 44-45 0.6211922232980811 1103 46-47 0.3757426258561196 1103 48-49 0.3299597041930866 1103 50-51 0.4217019034023224 1103 52-53 0.47650878195786106 1103 54-55 0.5926024139604493 1103 56-57 0.5240565990719901 1103 58-59 0.5077716767981357 1103 60-61 0.7024033389905071 1103 62-63 0.7132052505520861 1103 64-65 0.6830038958392777 1103 66-67 0.6990348150682308 1103 68-69 0.6214291603002806 1103 70-71 0.41340496435930163 1103 72-73 0.44450394548013605 1103 74-75 0.6114719950217555 1103 76-77 0.62892584540214 1103 78-79 0.7019801257582081 1103 80-81 0.5510587116376549 1103 82-83 0.5319694714486438 1103 84-85 0.5157034447328002 1103 86-87 0.2934556432933739 1103 88-89 0.4025008119291691 1103 90-91 0.597933350073923 1103 92-93 0.5400730382620686 1103 94-95 0.6810754464626214 1103 96-97 0.6089412975847992 1103 98-99 0.6703722959358949 1103 100-101 0.9048681036729356 1104 1 0.26216339028118796 1104 2 0.24454264858197305 1104 3 0.2790314668995606 1104 4 0.4213267121911102 1104 5 0.4262937038205834 1104 6 0.24588195764036413 1104 7 0.32522883132592995 1104 8 0.33614323802805046 1104 9 0.3318231431667584 1104 10-11 0.37751200341462976 1104 12-13 0.25805227600883285 1104 14-15 0.20759557194896416 1104 16-17 0.3718148710941236 1104 18-19 0.4398127212571197 1104 20-21 0.4007142993693691 1104 22-23 0.23559840384180575 1104 24-25 0.32540963544090573 1104 26-27 0.3286910430509735 1104 28-29 0.4667629910605342 1104 30-31 0.6150612017287287 1104 32-33 0.23511016686788366 1104 34-35 0.2783515594323305 1104 36-37 0.2832759790066035 1104 38-39 0.42142189594583357 1104 40-41 0.571230885772728 1104 42-43 0.6096897021790966 1104 44-45 0.48079138934159005 1104 46-47 0.3654184525080595 1104 48-49 0.40736500506616835 1104 50-51 0.465737802158813 1104 52-53 0.5915430266676296 1104 54-55 0.6931674640523084 1104 56-57 0.7424508118893698 1104 58-59 0.7191386671759332 1104 60-61 0.7618549284105569 1104 62-63 0.6640809296274099 1104 64-65 0.5663434607358511 1104 66-67 0.4911288184060396 1104 68-69 0.49923212569900954 1104 70-71 0.5279935078572677 1104 72-73 0.5597536124422859 1104 74-75 0.5139086187855533 1104 76-77 0.3848753375503655 1104 78-79 0.6374345429048311 1104 80-81 0.6713893724144633 1104 82-83 0.4961146660467435 1104 84-85 0.4414310519610858 1104 86-87 0.8127142688498843 1104 88-89 0.4162185047816074 1104 90-91 0.31778413921268367 1104 92-93 0.3950622919106266 1104 94-95 0.5563236772764242 1104 96-97 0.44160896790456405 1104 98-99 0.1086494874239925 1104 100-101 0.2741105106575681 1105 1 0.2002463798468277 1105 2 0.19901729065913898 1105 3 0.2944937333397455 1105 4 0.29090691117193757 1105 5 0.24252520102999853 1105 6 0.23696421922736022 1105 7 0.20786896721527626 1105 8 0.2046533083993225 1105 9 0.3468461958238862 1105 10-11 0.36295121719769696 1105 12-13 0.2850809098355711 1105 14-15 0.4583041378310284 1105 16-17 0.4384516097497908 1105 18-19 0.34203669602052145 1105 20-21 0.30426926175714186 1105 22-23 0.26925770983550024 1105 24-25 0.3009228603863079 1105 26-27 0.5158616327136798 1105 28-29 0.3196785455376059 1105 30-31 0.34246353126039963 1105 32-33 0.27967580627579736 1105 34-35 0.03211278243644955 1105 36-37 0.22077537235772837 1105 38-39 0.2062847933008456 1105 40-41 0.1104313225599114 1105 42-43 0.3036268533559934 1105 44-45 0.3362864148208402 1105 46-47 0.2723281831559561 1105 48-49 0.17933176070800982 1105 50-51 0.11416658158128001 1105 52-53 0.22196404098455247 1105 54-55 0.30870131505935205 1105 56-57 0.3018744954613126 1105 58-59 0.2902913000320453 1105 60-61 0.5247110312982031 1105 62-63 0.5184014828911714 1105 64-65 0.6457040528251525 1105 66-67 0.4771431353194089 1105 68-69 0.25894093424062703 1105 70-71 0.35459020768740146 1105 72-73 0.49058714799190994 1105 74-75 0.5385834549903556 1105 76-77 0.454420350896644 1105 78-79 0.6938953533877203 1105 80-81 0.5460508623440923 1105 82-83 0.3923619361268216 1105 84-85 0.2769357838222817 1105 86-87 0.5571840577360732 1105 88-89 0.5847771070626067 1105 90-91 0.3743383736218071 1105 92-93 0.4664466646356935 1105 94-95 0.5641288216588549 1105 96-97 0.3124813289819741 1105 98-99 0.3934335204256918 1105 100-101 0.6106137865614762 1106 1 0.49152594170891106 1106 2 0.45181041986003834 1106 3 0.5216682416793041 1106 4 0.44523103365140315 1106 5 0.3202249769822032 1106 6 0.5254879931880225 1106 7 0.3993128307950613 1106 8 0.548066303171538 1106 9 0.6606823919901785 1106 10-11 0.7496228627042925 1106 12-13 0.7494461077444612 1106 14-15 0.6554749120406385 1106 16-17 0.7960642560032127 1106 18-19 0.8755803853409105 1106 20-21 0.8100011512268921 1106 22-23 0.6710700075701226 1106 24-25 0.6011643341229274 1106 26-27 0.4809388047326024 1106 28-29 0.5567385405587686 1106 30-31 0.712858104324873 1106 32-33 0.7642887014712159 1106 34-35 0.6485865190558258 1106 36-37 0.44367305719715944 1106 38-39 0.4045758064947691 1106 40-41 0.7771689376955848 1106 42-43 0.5780405949542029 1106 44-45 0.4507399871384976 1106 46-47 0.5794292536065413 1106 48-49 0.258451750750325 1106 50-51 0.4851897331869637 1106 52-53 0.5785597591822054 1106 54-55 0.8281237696274317 1106 56-57 0.6208886851551156 1106 58-59 0.9129101698353779 1106 60-61 0.8777829755351974 1106 62-63 0.8751515451266769 1106 64-65 0.630122773263281 1106 66-67 0.5984762197107472 1106 68-69 0.7240529581390618 1106 70-71 0.6027704416928827 1106 72-73 0.7041034536926887 1106 74-75 0.8609334674374587 1106 76-77 0.8307422328981353 1106 78-79 0.7839563441768682 1106 80-81 1.0091240512910638 1106 82-83 0.7508707735676978 1106 84-85 0.6310296349549702 1106 86-87 0.8318803485003237 1106 88-89 0.8024432758452811 1106 90-91 0.9031235366788124 1106 92-93 1.2293667542548121 1106 94-95 1.1990043506605943 1106 96-97 1.1575199149914326 1106 98-99 1.082177608053275 1106 100-101 1.4363643466809677 1107 1 0.3461232535745431 1107 2 0.28849418906145985 1107 3 0.225249393076961 1107 4 0.1867251827159535 1107 5 0.15819727554730179 1107 6 0.2246423390225587 1107 7 0.2791162684304993 1107 8 0.1734195782161123 1107 9 0.16654824998463624 1107 10-11 0.26476665116228304 1107 12-13 0.3504895829587653 1107 14-15 0.2159319831212656 1107 16-17 0.402333541771263 1107 18-19 0.4068281956195605 1107 20-21 0.2738084635337117 1107 22-23 0.4182392655692553 1107 24-25 0.6432095792808852 1107 26-27 0.6597666971760887 1107 28-29 0.5619390476657955 1107 30-31 0.5117236015830215 1107 32-33 0.4322046447188299 1107 34-35 0.40373544605991896 1107 36-37 0.40090244656659735 1107 38-39 0.5260540857565914 1107 40-41 0.6308822509418732 1107 42-43 0.7418823588769357 1107 44-45 0.7485962661563548 1107 46-47 0.5532408350589932 1107 48-49 0.5420083383404801 1107 50-51 0.5137378603426228 1107 52-53 0.4381249810869505 1107 54-55 0.5832392831057334 1107 56-57 0.6579037348666574 1107 58-59 0.6112681059045926 1107 60-61 0.7326605717337102 1107 62-63 0.763082255206271 1107 64-65 0.8550853371382701 1107 66-67 0.9385603828673723 1107 68-69 0.6751530741456335 1107 70-71 0.6320033839257562 1107 72-73 0.5719013790695726 1107 74-75 0.8799590641446393 1107 76-77 0.855828653900776 1107 78-79 0.8845806905044924 1107 80-81 0.8282092732996169 1107 82-83 0.9398784372680211 1107 84-85 0.8927697854261325 1107 86-87 0.9509453926321321 1107 88-89 0.5917735909315844 1107 90-91 0.7238103371033944 1107 92-93 0.8360382999450486 1107 94-95 0.6792040792000265 1107 96-97 0.7139547112532725 1107 98-99 1.1363383381420569 1107 100-101 1.2662531672289248 1108 1 0.010257895277504758 1108 2 0.1299177973380914 1108 3 0.10928184941528585 1108 4 0.11441451135618763 1108 5 0.07316085280337603 1108 6 0.21462428371376774 1108 7 0.17724483087257425 1108 8 0.11880346961535082 1108 9 -0.02811234862567602 1108 10-11 -0.16733206239696585 1108 12-13 0.13425295036989127 1108 14-15 0.17650082138699474 1108 16-17 0.24341591191468837 1108 18-19 0.17359358224806698 1108 20-21 0.1901571014623471 1108 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-5.232002597866444 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2627.0 3 925.0 4 983.0 5 1066.0 6 1192.0 7 1461.0 8 1756.0 9 2173.0 10 2742.0 11 3466.0 12 4303.0 13 5336.0 14 6133.0 15 7223.0 16 8996.0 17 11635.0 18 10691.0 19 7088.0 20 6906.0 21 7395.0 22 8051.0 23 9032.0 24 10053.0 25 11792.0 26 13593.0 27 16068.0 28 19164.0 29 23416.0 30 28789.0 31 35303.0 32 44412.0 33 57310.0 34 76824.0 35 108894.0 36 161446.0 37 238100.0 38 218745.0 39 39283.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.57239178445775 19.350908568194715 14.777464839782429 22.2992348075651 2 15.125756246628036 20.761579994316566 40.4633233255495 23.649340433505895 3 19.141439308143728 25.419129002367963 30.170534335426748 25.268897354061565 4 12.544991551597864 17.139834854540016 38.15711968391195 32.15805390995017 5 13.466480930439584 37.759460595619814 34.173295196960076 14.600763276980523 6 30.714431168282974 36.78293682102575 17.962638226935795 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21.425547964608764 27.159329727450604 29.936046090296397 21.47907621764423 30-31 21.64610734383486 26.834311718011104 29.875779389730067 21.64380154842397 32-33 21.480831825284575 26.98244075375674 29.78866729665018 21.74806012430851 34-35 22.0137403417174 26.909040749563022 29.60543354957455 21.471785359145027 36-37 21.87546579682241 26.726629043322298 29.644153874153123 21.75375128570217 38-39 21.665868568706355 27.076066901882534 29.669480390877222 21.588584138533882 40-41 21.83859297756996 26.972788639464913 29.63356114465099 21.555057238314134 42-43 21.655086373546286 27.04345337722522 29.31076154066278 21.990698708565713 44-45 21.87081314102432 27.149003800340093 29.106199536383215 21.87398352225237 46-47 22.121406634779166 27.303749628923885 28.764585195576153 21.810258540720792 48-49 22.107276301839406 27.457504297433893 28.519932887978506 21.915286512748196 50-51 21.81624365356831 27.648064191652665 28.56512897497331 21.970563179805715 52-53 22.17457328004704 27.78795722053724 28.197486496568864 21.839983002846857 54-55 21.887542510966686 27.612167391190663 28.331914756941657 22.168375340900994 56-57 22.07278894729972 27.912655239701508 28.07538387611212 21.939171936886652 58-59 22.324358599562725 27.493360563600653 28.21655473983703 21.965726096999592 60-61 22.1147262283317 27.559237969285068 28.592580209912953 21.733455592470275 62-63 21.985694409158345 27.646083421417046 28.611799333020667 21.756422836403942 64-65 21.831087980500016 27.681988866563458 28.379269738792452 22.107653414144078 66-67 21.86758079813264 27.872663485491362 28.081085789189963 22.178669927186036 68-69 21.787839056341156 27.78040719920483 28.337734696458355 22.094019047995662 70-71 22.004581146440135 27.602107656941342 28.225712096368866 22.167599100249653 72-73 21.966844704886416 27.5784661043887 28.33706373636509 22.117625454359796 74-75 21.865562426856876 28.116442615025665 27.89280275796411 22.12519220015335 76-77 21.980751728596903 27.776029848410598 27.98202012132094 22.261198301671563 78-79 21.95641457427054 27.85284719485281 27.969476941121336 22.22126128975532 80-81 22.00318833640781 27.98265821882503 27.86160197217025 22.15255147259691 82-83 22.06657710425674 27.832356301341544 28.048904041098766 22.05216255330295 84-85 21.97374083018837 27.986131271142295 27.802724744027657 22.23740315464168 86-87 22.030753516761635 27.754853244316855 28.086688097066947 22.12770514185456 88-89 22.022688514866168 27.75944736907145 27.81427382747172 22.403590288590657 90-91 21.93653250710231 27.82211043974806 27.99618244360681 22.24517460954282 92-93 22.09550140171864 27.862648873393876 27.905569555808007 22.136280169079477 94-95 21.89267048907878 27.692049854971252 27.943894215485876 22.471385440464093 96-97 21.772695470768387 27.69627171354083 28.135164951463548 22.395867864227238 98-99 21.683759103117662 27.81635972497783 27.839919402836305 22.6599617690682 100-101 21.681989141864474 27.835797894958915 27.93114445539561 22.551068507781004 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 304.0 1 230.5 2 195.5 3 248.5 4 233.0 5 263.0 6 452.5 7 878.0 8 1537.5 9 1886.0 10 1641.0 11 1363.0 12 1401.5 13 1504.5 14 1561.5 15 1640.0 16 1758.5 17 1933.5 18 2035.0 19 2121.0 20 2385.5 21 2807.5 22 3305.0 23 3982.5 24 4779.0 25 5918.5 26 7588.0 27 9570.5 28 11282.0 29 13057.5 30 15309.5 31 17693.5 32 20237.0 33 22743.0 34 25581.5 35 28081.5 36 31208.5 37 34478.0 38 36284.0 39 37243.0 40 37888.5 41 39581.0 42 41173.0 43 42421.5 44 43607.0 45 44910.0 46 45301.0 47 46203.0 48 50250.5 49 50210.5 50 45594.5 51 42106.0 52 39708.5 53 38293.0 54 36490.5 55 33299.0 56 30184.5 57 27207.5 58 24510.0 59 22029.5 60 18673.5 61 14866.5 62 11476.5 63 8754.5 64 6553.5 65 4862.0 66 3474.0 67 2536.0 68 1787.0 69 1134.0 70 786.5 71 580.5 72 433.5 73 308.5 74 202.5 75 147.5 76 100.5 77 50.0 78 36.5 79 20.5 80 10.5 81 11.5 82 8.5 83 6.0 84 7.5 85 6.5 86 3.5 87 3.5 88 2.0 89 0.5 90 2.0 91 2.0 92 1.0 93 1.0 94 1.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03705554956262099 2 0.027832835004813105 3 0.022068638406183172 4 0.045619498794871184 5 0.09906183583074013 6 0.11874244993177663 7 0.0592888793001936 8 0.09016850393571109 9 0.07526393844496798 10-11 0.0765814690960834 12-13 0.0744816546208682 14-15 0.0664117793827863 16-17 0.05220715205044824 18-19 0.06352968108347133 20-21 0.0590006694702621 22-23 0.033844068600527175 24-25 0.01000499838190767 26-27 0.002429197137994043 28-29 0.006669998921271779 30-31 0.005064258440224869 32-33 0.006217097759950855 34-35 0.007040554416897989 36-37 0.006258270592798213 38-39 0.003911419120498883 40-41 0.006917035918355918 42-43 0.003746727789109456 44-45 0.002305678639451973 46-47 0.0016057404810469098 48-49 4.5290116132092333E-4 50-51 0.002429197137994043 52-53 0.00592888793001936 54-55 0.012145985689970216 56-57 0.008358085068013402 58-59 0.004776048610293373 60-61 0.002676234135078183 62-63 0.009263887390655248 64-65 0.00271740696792554 66-67 0.022356848236114668 68-69 0.04648412828466567 70-71 0.05953591629727774 72-73 0.0038702462876515262 74-75 0.013504689173932986 76-77 0.021245181749236036 78-79 0.024250798547093076 80-81 0.07472869161795234 82-83 0.028162217667591957 84-85 0.012393022687054354 86-87 0.03174425412531199 88-89 0.0463606097861236 90-91 0.04343733865396128 92-93 0.04331382015541921 94-95 0.01243419551990171 96-97 0.016674997303179447 98-99 0.03742610505824721 100-101 0.07616974076760982 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1214393.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.70891644853683 #Duplication Level Percentage of deduplicated Percentage of total 1 83.98253667093516 56.02384021917594 2 10.032433021535958 13.385054724183682 3 2.6315732911926384 5.266482084311123 4 1.0944753648979606 2.920450626878396 5 0.5633461635819036 1.8790106078994477 6 0.3407986581699189 1.3640585528178346 7 0.23934834506873795 1.11766681273002 8 0.1583127612258757 0.8448698209083281 9 0.12781146210674543 0.7673547732159789 >10 0.7470533653949852 9.206534021625504 >50 0.054785543099010806 2.479922425382019 >100 0.025797313963159405 2.9089090962736 >500 9.874507588410368E-4 0.4205912525263259 >1k 6.17156724275648E-4 0.9434484282393583 >5k 1.234313448551296E-4 0.4718065538323899 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5730 0.4718406644307073 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4241 0.3492279682112792 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2676 0.22035700139905284 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2084 0.17160836730778256 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1307 0.10762578506299032 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 8.234566569471333E-5 0.0 14-15 0.0 0.0 0.0 8.234566569471333E-5 0.0 16-17 0.0 0.0 0.0 1.6469133138942665E-4 0.0 18-19 4.1172832847356664E-5 0.0 0.0 2.0586416423678332E-4 0.0 20-21 8.234566569471333E-5 0.0 0.0 4.5290116132092333E-4 0.0 22-23 8.234566569471333E-5 0.0 0.0 0.0010293208211839165 0.0 24-25 8.234566569471333E-5 0.0 0.0 0.0013175306511154132 0.0 26-27 8.234566569471333E-5 0.0 0.0 0.0015233948153521965 0.0 28-29 8.234566569471333E-5 0.0 0.0 0.0024703699708413995 0.0 30-31 8.234566569471333E-5 0.0 0.0 0.004487838780361877 0.0 32-33 8.234566569471333E-5 0.0 0.0 0.006258270592798213 0.0 34-35 8.234566569471333E-5 0.0 0.0 0.00876981339648697 0.0 36-37 8.234566569471333E-5 0.0 0.0 0.011898948692886074 0.0 38-39 8.234566569471333E-5 0.0 0.0 0.013463516341085629 0.0 40-41 8.234566569471333E-5 0.0 0.0 0.015933886311927027 0.0 42-43 8.234566569471333E-5 0.0 0.0 0.020792280587915116 0.0 44-45 8.234566569471333E-5 0.0 0.0 0.02737993384349218 0.0 46-47 8.234566569471333E-5 0.0 0.0 0.04331382015541921 0.0 48-49 8.234566569471333E-5 4.1172832847356664E-5 0.0 0.07810486391143559 0.0 50-51 8.234566569471333E-5 8.234566569471333E-5 0.0 0.09906183583074013 0.0 52-53 8.234566569471333E-5 8.234566569471333E-5 0.0 0.11701319095218764 0.0 54-55 8.234566569471333E-5 8.234566569471333E-5 0.0 0.141181643833586 0.0 56-57 8.234566569471333E-5 8.234566569471333E-5 0.0 0.1608622579346225 0.0 58-59 8.234566569471333E-5 8.234566569471333E-5 0.0 0.18391904432914222 0.0 60-61 8.234566569471333E-5 1.6469133138942665E-4 0.0 0.22150984071877883 0.0 62-63 8.234566569471333E-5 2.4703699708414E-4 0.0 0.25247181101999105 0.0 64-65 8.234566569471333E-5 2.4703699708414E-4 0.0 0.28569828712780787 0.0 66-67 8.234566569471333E-5 2.4703699708414E-4 0.0 0.34823982022294264 0.0 68-69 8.234566569471333E-5 2.4703699708414E-4 0.0 0.42066283320144304 0.0 70-71 8.234566569471333E-5 2.4703699708414E-4 0.0 0.4789635645133 0.0 72-73 1.2351849854207E-4 2.4703699708414E-4 0.0 0.5447165785705287 8.234566569471333E-5 74-75 1.6469133138942665E-4 2.4703699708414E-4 0.0 0.6035525567094013 8.234566569471333E-5 76-77 1.6469133138942665E-4 2.4703699708414E-4 0.0 0.6504895861553879 8.234566569471333E-5 78-79 1.6469133138942665E-4 2.4703699708414E-4 0.0 0.70590821916793 8.234566569471333E-5 80-81 1.6469133138942665E-4 2.4703699708414E-4 0.0 0.8087167827877796 8.234566569471333E-5 82-83 1.6469133138942665E-4 2.4703699708414E-4 0.0 0.9254005910771883 8.234566569471333E-5 84-85 1.6469133138942665E-4 2.4703699708414E-4 0.0 1.0480132872966166 8.234566569471333E-5 86-87 1.6469133138942665E-4 2.4703699708414E-4 0.0 1.2518188098910321 8.234566569471333E-5 88-89 1.6469133138942665E-4 2.4703699708414E-4 0.0 1.4993498809693402 8.234566569471333E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7865 0.0 30.346083 1 GGTATCA 5445 0.0 27.766867 1 ATCAACG 9230 0.0 25.6533 3 TCAACGC 9265 0.0 25.557444 4 CAACGCA 9320 0.0 25.456587 5 TATCAAC 9350 0.0 25.37491 2 AACGCAG 9605 0.0 24.698183 6 GAGTACT 4520 0.0 23.980005 12-13 GTGTTCG 140 1.7702061E-5 23.766224 9 GTACATG 5585 0.0 22.303642 1 ACGCAGA 10600 0.0 22.2416 7 CGCAGAG 10615 0.0 21.987183 8 GTACTTT 4945 0.0 21.96665 14-15 TACATGG 5645 0.0 20.972538 2 AGAGTAC 7860 0.0 20.77277 10-11 ACATGGG 5455 0.0 20.657091 3 GCAGAGT 10970 0.0 20.624838 9 AGTACTT 4830 0.0 20.472414 12-13 GTATAGG 335 3.45608E-11 19.869223 1 GTGTAGC 575 0.0 19.84454 1 >>END_MODULE