FastQCFastQC Report
Thu 26 May 2016
SRR936872_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936872_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214393
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60160.49539152481939536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51710.42580943730736265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31690.26095341458654653No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22790.18766577211825167No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14310.11783664760913477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA79400.028.7829361
GGTATCA55700.028.6612171
ATCAACG95150.023.8119473
TCAACGC96250.023.539814
TATCAAC97450.023.3971312
CAACGCA97150.023.2728445
AACGCAG100550.022.4423546
TACACCG1100.002877777721.5904965
GTACATG52000.021.3807541
GAGTACT56250.020.9853412-13
TACATGG52350.020.8696122
ACATGGG50800.020.4769733
AGAGTAC84800.020.28007510-11
ACGCAGA112700.019.9807267
AGTACTT58900.019.9605312-13
GTACTTT59400.019.75049414-15
CGCAGAG112450.019.7294188
GTGTAGC6950.019.1418421
TAAGGTG4550.018.790855
GCAGAGT117650.018.4132219
Produced by FastQC (version 0.11.5)