##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936872_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1214393 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07412509788841 31.0 31.0 34.0 31.0 34.0 2 32.212660975483224 33.0 31.0 34.0 30.0 34.0 3 32.333112097978166 34.0 31.0 34.0 30.0 34.0 4 36.01728435522932 37.0 35.0 37.0 35.0 37.0 5 35.85217882514145 37.0 35.0 37.0 35.0 37.0 6 35.936837580585525 37.0 35.0 37.0 35.0 37.0 7 35.89509079844828 37.0 35.0 37.0 35.0 37.0 8 35.84048491715614 37.0 35.0 37.0 35.0 37.0 9 37.52377360541439 39.0 37.0 39.0 35.0 39.0 10-11 37.43567691842756 39.0 37.0 39.0 34.0 39.0 12-13 37.328454215398146 39.0 37.0 39.0 34.0 39.0 14-15 38.50515977941243 40.0 38.0 41.0 34.0 41.0 16-17 38.50154933370005 40.0 38.0 41.0 34.0 41.0 18-19 38.642993248478874 40.0 38.0 41.0 34.0 41.0 20-21 38.651167290984056 40.0 38.0 41.0 34.0 41.0 22-23 38.559310700901605 40.0 38.0 41.0 34.0 41.0 24-25 38.4672350713484 40.0 38.0 41.0 34.0 41.0 26-27 38.26817389428299 40.0 38.0 41.0 34.0 41.0 28-29 38.112719276214534 40.0 38.0 41.0 33.0 41.0 30-31 38.09689614482296 40.0 37.5 41.0 33.5 41.0 32-33 37.967911129263754 40.0 37.0 41.0 33.0 41.0 34-35 37.84085794302174 40.0 37.0 41.0 33.0 41.0 36-37 37.68455928187991 40.0 37.0 41.0 33.0 41.0 38-39 37.52919936132702 40.0 37.0 41.0 33.0 41.0 40-41 37.210613450505726 40.0 36.0 41.0 31.5 41.0 42-43 37.011239359910675 39.0 36.0 41.0 31.0 41.0 44-45 36.78521327115686 39.0 36.0 41.0 31.0 41.0 46-47 36.41374991456637 39.0 35.5 40.5 30.0 41.0 48-49 36.03531188009154 39.0 35.0 40.0 29.5 41.0 50-51 35.759051229709 39.0 35.0 40.0 28.5 41.0 52-53 35.50020792280588 38.5 35.0 40.0 28.0 41.0 54-55 35.11524564123805 38.0 34.0 40.0 27.0 41.0 56-57 34.91454660888197 38.0 34.0 40.0 26.5 41.0 58-59 34.59459252482516 38.0 34.0 40.0 26.0 41.0 60-61 34.21642870141709 37.5 33.5 40.0 26.0 41.0 62-63 33.854931640745626 37.0 33.0 39.5 25.0 40.5 64-65 33.52600434949806 36.0 33.0 39.0 24.0 40.0 66-67 33.18881572933968 36.0 32.0 39.0 23.0 40.0 68-69 32.73333838386749 35.5 32.0 38.5 22.0 40.0 70-71 32.27320974346855 35.0 31.0 38.0 21.5 40.0 72-73 31.853343192854375 35.0 31.0 37.0 20.5 39.0 74-75 31.362605433331716 34.5 31.0 37.0 19.0 39.0 76-77 29.936780762076197 33.5 28.5 35.0 18.0 37.5 78-79 30.62032142807147 34.0 30.0 36.0 18.0 38.0 80-81 30.47205558661817 34.0 30.0 35.5 17.5 37.0 82-83 30.10441595101421 34.0 30.0 35.0 13.5 37.0 84-85 29.642473647328337 34.0 29.0 35.0 7.5 36.0 86-87 29.251985559864064 34.0 29.0 35.0 7.0 36.0 88-89 28.859872792415636 34.0 29.0 35.0 2.0 35.5 90-91 28.376116298430574 33.0 29.0 35.0 2.0 35.0 92-93 27.864085184944248 33.0 27.0 35.0 2.0 35.0 94-95 27.262766254416817 33.0 26.5 34.0 2.0 35.0 96-97 26.454467375882437 32.0 25.0 34.0 2.0 35.0 98-99 25.537059255117576 32.0 23.5 34.0 2.0 35.0 100-101 22.238426522550775 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2684790370190768 1101 2 0.35479290442180655 1101 3 0.25317712203689524 1101 4 0.16302913192394897 1101 5 0.16092095912902238 1101 6 0.19697270076584772 1101 7 0.25947647683805286 1101 8 0.26001548816381614 1101 9 0.32574960846546475 1101 10-11 0.3428443212711869 1101 12-13 0.30819291566337625 1101 14-15 0.36169804642884174 1101 16-17 0.26031623003382975 1101 18-19 0.25909259130370543 1101 20-21 0.12666088237415352 1101 22-23 0.4369901264904854 1101 24-25 0.30717655592069093 1101 26-27 0.3048702332018891 1101 28-29 0.26889346791605107 1101 30-31 0.39608270305804893 1101 32-33 0.39202531975514887 1101 34-35 0.3096470410051282 1101 36-37 0.4052596237883819 1101 38-39 0.3127606673920127 1101 40-41 0.5537543257695532 1101 42-43 0.41765166975664414 1101 44-45 0.28359648025180917 1101 46-47 0.1931760837196137 1101 48-49 0.31893045964218913 1101 50-51 0.40073188423925643 1101 52-53 0.4762772461301594 1101 54-55 0.17347258178070746 1101 56-57 0.39327910197318516 1101 58-59 0.4526692388229989 1101 60-61 0.2276020494313329 1101 62-63 0.316333304189385 1101 64-65 0.46941971458846865 1101 66-67 0.44656035355578894 1101 68-69 -0.024307900109469927 1101 70-71 0.11888574116822781 1101 72-73 0.22928862379968606 1101 74-75 0.21497470442648137 1101 76-77 0.18246960406619195 1101 78-79 0.22130873850688104 1101 80-81 0.09228715487522976 1101 82-83 0.46140690693247777 1101 84-85 0.37192610255584313 1101 86-87 -0.10860520768404669 1101 88-89 0.1103628489221471 1101 90-91 0.3650310680780855 1101 92-93 0.09332194730207632 1101 94-95 0.09443552704372493 1101 96-97 0.004633545786422388 1101 98-99 0.13337279245067535 1101 100-101 0.6723189005595955 1102 1 0.0324338270676634 1102 2 0.12457667611821677 1102 3 0.15771191479063873 1102 4 0.1094658474396013 1102 5 -0.07050442943010182 1102 6 0.1605646747692404 1102 7 0.22350548852796237 1102 8 0.3172966515676521 1102 9 0.3032024060164247 1102 10-11 0.2850530540046279 1102 12-13 0.2759139782290916 1102 14-15 0.3726216096929349 1102 16-17 0.24392561757614573 1102 18-19 0.20277182331361843 1102 20-21 0.2712000523016158 1102 22-23 0.12477937509152781 1102 24-25 0.1760754452468447 1102 26-27 0.16012002097013323 1102 28-29 0.07876848057519226 1102 30-31 0.2491551187753558 1102 32-33 0.16381688200515754 1102 34-35 0.3210324504713853 1102 36-37 0.3761337467393844 1102 38-39 0.4648657549049062 1102 40-41 0.43020248038981634 1102 42-43 0.4689971409111351 1102 44-45 0.4715587087769748 1102 46-47 0.537626350801979 1102 48-49 0.6190390733627282 1102 50-51 0.5362444927425258 1102 52-53 0.5743266171153394 1102 54-55 0.8315643379449469 1102 56-57 0.6511333760726146 1102 58-59 0.42260029833693125 1102 60-61 0.46694169432291943 1102 62-63 0.3313088117274319 1102 64-65 0.6131493792520999 1102 66-67 0.586220402126095 1102 68-69 0.6693218847904987 1102 70-71 0.3656094878114331 1102 72-73 0.33896707145269644 1102 74-75 0.3920548304945193 1102 76-77 0.39357308004509406 1102 78-79 0.12292292326846876 1102 80-81 0.24097342502705388 1102 82-83 0.267929031710473 1102 84-85 0.21722393728218847 1102 86-87 0.31765980571595165 1102 88-89 0.26810728586739785 1102 90-91 0.3075854208171158 1102 92-93 0.46013522210090585 1102 94-95 0.7356449183922784 1102 96-97 1.0037108729101476 1102 98-99 0.9527922867783616 1102 100-101 0.9091987641331514 1103 1 0.1438905866379656 1103 2 0.24645829875909442 1103 3 0.21100560159676007 1103 4 0.07790116168732197 1103 5 0.019541023306622662 1103 6 0.018816573553785076 1103 7 0.0925820958360859 1103 8 0.14706004318129118 1103 9 0.16574799032348153 1103 10-11 0.15376704063708502 1103 12-13 0.28637670788351244 1103 14-15 0.31354364175502525 1103 16-17 0.2874651291100534 1103 18-19 0.2634292770618387 1103 20-21 0.213739757661088 1103 22-23 0.14153501753342113 1103 24-25 0.17024944669219622 1103 26-27 0.23153447265558214 1103 28-29 0.1768949190240221 1103 30-31 0.21169640297883774 1103 32-33 0.3875503089566621 1103 34-35 0.33600611715045403 1103 36-37 0.20451051539766496 1103 38-39 0.1809019964346561 1103 40-41 0.2997363748331878 1103 42-43 0.2680237771647356 1103 44-45 0.19844436838078394 1103 46-47 0.3107840131373081 1103 48-49 0.28756590921814507 1103 50-51 0.2580107827543472 1103 52-53 0.4654813682163592 1103 54-55 0.6606814304074433 1103 56-57 0.4101260479259352 1103 58-59 0.3396599645205285 1103 60-61 0.21824488243255047 1103 62-63 0.44228967177382117 1103 64-65 0.28949341834582043 1103 66-67 0.40926054969025216 1103 68-69 0.4654077636354046 1103 70-71 0.28390784055086016 1103 72-73 0.5292575659132659 1103 74-75 0.3497430948058451 1103 76-77 0.4360311476170473 1103 78-79 0.509019276395545 1103 80-81 0.4550569702634988 1103 82-83 0.728900457201231 1103 84-85 0.43947896294803357 1103 86-87 0.15887398809939057 1103 88-89 0.6017648384538141 1103 90-91 0.23503978516553659 1103 92-93 0.10017034175288941 1103 94-95 0.35670534960923916 1103 96-97 0.4013125703599947 1103 98-99 0.2009612128178624 1103 100-101 0.5875853524309633 1104 1 0.11412517404244937 1104 2 0.1855878938147839 1104 3 0.06926518788699099 1104 4 0.05782356015410528 1104 5 0.03070955283928356 1104 6 0.08324020760338158 1104 7 0.18792625145513853 1104 8 0.24635484481949277 1104 9 0.13421215346993876 1104 10-11 0.21285154558684383 1104 12-13 0.2687207496011368 1104 14-15 0.3315666845076706 1104 16-17 0.36686308581760585 1104 18-19 0.38361075173857984 1104 20-21 0.34788096570945726 1104 22-23 0.3459335932704306 1104 24-25 0.1842710830099108 1104 26-27 0.26979854071910125 1104 28-29 0.422324624514566 1104 30-31 0.5108300093454332 1104 32-33 0.4164168907750181 1104 34-35 0.5458490569956425 1104 36-37 0.3372248605387753 1104 38-39 0.36240948173890075 1104 40-41 0.5962719145525881 1104 42-43 0.35876792486021003 1104 44-45 0.6482280794871613 1104 46-47 0.6808367296861917 1104 48-49 0.740840327454265 1104 50-51 0.8691890929524888 1104 52-53 0.8954189948078835 1104 54-55 0.9721714838420681 1104 56-57 0.9103581839737558 1104 58-59 0.5324077867748898 1104 60-61 0.810461082185931 1104 62-63 0.7534578298888022 1104 64-65 0.5372741977286708 1104 66-67 0.45956412173784855 1104 68-69 0.7182039197100636 1104 70-71 0.770632753762925 1104 72-73 0.7025294832703572 1104 74-75 0.5051752021306974 1104 76-77 0.7988604093872738 1104 78-79 0.9629814363626608 1104 80-81 0.8607144043121018 1104 82-83 0.9605976131322755 1104 84-85 0.7685921661035984 1104 86-87 0.6393615604639322 1104 88-89 0.8005203416257203 1104 90-91 0.8513622301833372 1104 92-93 0.8521871397363405 1104 94-95 0.8192163064306932 1104 96-97 1.0964770836295088 1104 98-99 0.6066477412511233 1104 100-101 0.9803143561897123 1105 1 0.08046829464424121 1105 2 0.18707054365705034 1105 3 0.12778739123569238 1105 4 0.1222496703215441 1105 5 0.10771998719987153 1105 6 0.19985268030417558 1105 7 0.25138900321442037 1105 8 0.23737401492383725 1105 9 0.2777574094757611 1105 10-11 0.2685237125366129 1105 12-13 0.2939512761723577 1105 14-15 0.3615970169512508 1105 16-17 0.23533797055431904 1105 18-19 0.16430839794095675 1105 20-21 0.3106157388227757 1105 22-23 0.31030267844393933 1105 24-25 0.21111286898418768 1105 26-27 0.21647167830707303 1105 28-29 0.15621203048399224 1105 30-31 0.23492246263346317 1105 32-33 0.16131012057361005 1105 34-35 0.23701867602015625 1105 36-37 0.39459685762685837 1105 38-39 0.37360215342053493 1105 40-41 0.3777897500294145 1105 42-43 0.2255041539308209 1105 44-45 0.23787136995063918 1105 46-47 0.2859253004214537 1105 48-49 0.3186491117299255 1105 50-51 0.3450202019066282 1105 52-53 0.4885347434125933 1105 54-55 0.4827065481468438 1105 56-57 0.16260642469202935 1105 58-59 0.045876605022883155 1105 60-61 0.10399056532109086 1105 62-63 0.2774702055257876 1105 64-65 0.2434887087696822 1105 66-67 0.44292616978444244 1105 68-69 0.348461138831631 1105 70-71 -0.02011099775369729 1105 72-73 0.12381800547370503 1105 74-75 0.2188090288717781 1105 76-77 0.32490869863745786 1105 78-79 0.3169392136011915 1105 80-81 0.200606420812953 1105 82-83 0.3325425294147344 1105 84-85 -0.028111303927946807 1105 86-87 0.21980805403345371 1105 88-89 0.4241274758164515 1105 90-91 0.3941528149928537 1105 92-93 0.4689537012505376 1105 94-95 0.5686205772387503 1105 96-97 0.9251351763254583 1105 98-99 0.827028716742511 1105 100-101 0.9209840965439966 1106 1 0.08075457794023322 1106 2 0.1144491844337523 1106 3 0.09994571834548083 1106 4 0.16562781321425035 1106 5 0.050035147767459875 1106 6 0.12887433825787298 1106 7 0.16082141546236528 1106 8 0.23395604131168568 1106 9 0.21138947320491042 1106 10-11 0.27080153180472877 1106 12-13 0.3647566035310845 1106 14-15 0.4706933575039045 1106 16-17 0.29524710672558996 1106 18-19 0.39056306560339493 1106 20-21 0.2645029680983555 1106 22-23 0.262859312748148 1106 24-25 0.3987578776971574 1106 26-27 0.371194107980962 1106 28-29 0.453430103672936 1106 30-31 0.4171243545926373 1106 32-33 0.21911818629430257 1106 34-35 0.3702661243646972 1106 36-37 0.4233222746047076 1106 38-39 0.43194793385369223 1106 40-41 0.6909538028051188 1106 42-43 0.8375243182325391 1106 44-45 0.6579074665401308 1106 46-47 0.7830898510811437 1106 48-49 0.517012318102843 1106 50-51 0.6425867938906933 1106 52-53 0.6518506508061748 1106 54-55 0.8510585521299205 1106 56-57 0.7308090634771887 1106 58-59 0.8192124277761863 1106 60-61 0.6097423701505633 1106 62-63 0.7116163344777391 1106 64-65 0.5691683204201681 1106 66-67 0.6065134239542189 1106 68-69 0.6099236730611537 1106 70-71 0.6180978645813795 1106 72-73 0.5506888045775149 1106 74-75 0.42813315135123986 1106 76-77 0.5724342151921 1106 78-79 0.7205501172606397 1106 80-81 0.6731257138176829 1106 82-83 0.6996523128351946 1106 84-85 0.8367268837743183 1106 86-87 0.9006233390720411 1106 88-89 0.8679238412633019 1106 90-91 0.6615339451895146 1106 92-93 0.8155394617683349 1106 94-95 1.000668125160086 1106 96-97 1.1383415492405682 1106 98-99 1.401959012983486 1106 100-101 1.580742622807378 1107 1 0.12517250783282208 1107 2 0.2726599582520848 1107 3 0.21319421376360737 1107 4 0.10150758161633888 1107 5 -0.09735742936912573 1107 6 0.08345270497878232 1107 7 0.052111969101780176 1107 8 0.1306816455450175 1107 9 0.25320309450444256 1107 10-11 0.07107753401585626 1107 12-13 0.13902715057862736 1107 14-15 0.1574405799640246 1107 16-17 0.1804440713500739 1107 18-19 0.190258061749482 1107 20-21 0.20083657652557463 1107 22-23 0.042335557353325726 1107 24-25 0.1939122027698872 1107 26-27 0.2396216998973486 1107 28-29 0.209510716561347 1107 30-31 0.24546252802116442 1107 32-33 0.16979787580154238 1107 34-35 0.16709177453351032 1107 36-37 0.11771665282764587 1107 38-39 0.1176956701684233 1107 40-41 0.318607096275791 1107 42-43 0.24920750350826637 1107 44-45 0.2130521114224777 1107 46-47 0.13558016440269682 1107 48-49 0.20728028809165266 1107 50-51 -0.04561115951462824 1107 52-53 -0.07944718244022653 1107 54-55 0.04609622182520923 1107 56-57 0.23576724142140648 1107 58-59 0.14464719289532013 1107 60-61 0.09790457432732325 1107 62-63 0.1239460552574485 1107 64-65 0.42869720917064313 1107 66-67 0.18254217126492023 1107 68-69 -0.18238304104622927 1107 70-71 -0.024630768279884308 1107 72-73 0.09977846442133043 1107 74-75 0.1447676372241311 1107 76-77 -0.410156689062255 1107 78-79 -0.1747753629209221 1107 80-81 0.07669395396944978 1107 82-83 0.3688876037469768 1107 84-85 0.1639536519661995 1107 86-87 0.2436070177001035 1107 88-89 0.3017238609870141 1107 90-91 0.1642586073560608 1107 92-93 0.2148518568239126 1107 94-95 0.602966391809101 1107 96-97 0.4930196374937985 1107 98-99 0.6759791207891439 1107 100-101 1.2775706735596017 1108 1 0.015152771843688129 1108 2 0.11464539395645801 1108 3 0.05619826180225118 1108 4 0.10065823780196581 1108 5 -0.00990322790837439 1108 6 0.08110095298957987 1108 7 0.0731738949693721 1108 8 0.10512389976953074 1108 9 0.033664825413119104 1108 10-11 0.09214186085535658 1108 12-13 0.07879696940128866 1108 14-15 0.10796735320182904 1108 16-17 0.08175782315681346 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-2.5068966133229047 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 11.0 5 27.0 6 57.0 7 130.0 8 300.0 9 698.0 10 1164.0 11 1925.0 12 2807.0 13 3897.0 14 4984.0 15 6808.0 16 9026.0 17 12344.0 18 11291.0 19 7278.0 20 6289.0 21 6518.0 22 7039.0 23 7787.0 24 9031.0 25 11027.0 26 13461.0 27 16748.0 28 20908.0 29 25797.0 30 32149.0 31 40338.0 32 51159.0 33 66388.0 34 89385.0 35 125791.0 36 188856.0 37 265589.0 38 159643.0 39 7740.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.46592040641012 20.047391452242447 15.470912404881467 22.01577573646597 2 15.488266227916315 21.242582500382916 39.75458637403467 23.514564897666094 3 19.420202735529774 25.191001243422622 30.532695427333888 24.856100593713716 4 12.972324445216662 17.67903800499509 37.6379804560797 31.710657093708544 5 13.419107050803799 37.83077148583861 34.31483961072435 14.435281852633244 6 30.878883524526245 37.12167313217385 17.50924124233259 14.490202100967315 7 27.51301271668304 33.50911446002887 21.166611357645696 17.811261465642392 8 26.141866759772164 34.80915980246922 19.81780198008388 19.23117145767474 9 25.893945282906998 17.051413382169844 20.873655294171897 36.18098604075126 10-11 24.443652096150092 27.2137191172874 27.4871479002267 20.855480886335805 12-13 25.758177163152922 24.663908485935007 28.08996258833082 21.487951762581247 14-15 22.66052243027826 25.79512880415494 26.88973560623965 24.654613159327155 16-17 21.40180320538738 29.198620216025617 27.41451902308396 21.985057555503037 18-19 21.29173600880772 27.467598932467276 30.288276655997375 20.952388402727625 20-21 22.227355148544692 26.368926746279175 30.4224700300235 20.981248075152628 22-23 21.874641019486134 26.50026577424711 30.326673811622673 21.298419394644082 24-25 21.876204310319274 26.513766605071854 30.49025517379948 21.119773910809396 26-27 21.70044763140732 26.688128709675297 30.287007819550855 21.32441583936653 28-29 21.27976692882782 26.86654979071849 30.578115980576303 21.275567299877387 30-31 21.573576264026556 26.485454049883355 30.501658029978763 21.43931165611132 32-33 21.445975801862826 26.74995121011272 30.300908027151056 21.50316496087339 34-35 21.976534779103634 26.611648782560504 30.183721414731473 21.228095023604386 36-37 21.873962942193216 26.40630185289034 30.243467204886805 21.476268000029645 38-39 21.57652770257333 26.805088601705123 30.271497777494115 21.346885918227432 40-41 21.87839935440573 26.586379113707764 30.161316221791278 21.373905310095235 42-43 21.638711685591073 26.79462085173416 29.830499681734 21.736167780940765 44-45 21.85336992257382 26.796911187373006 29.611963281750192 21.737755608302987 46-47 22.127240836963413 27.05457060746548 29.223849010614384 21.59433954495673 48-49 22.107153209177927 27.25544964064322 28.89042417934242 21.746972970836435 50-51 21.942820157463196 27.38584906359547 28.83342020901218 21.837910569929157 52-53 22.257187265185124 27.57740012969501 28.480612654527487 21.684799950592375 54-55 21.99218045558563 27.341313726281356 28.673748942887517 21.992756875245494 56-57 22.119317419183773 27.6562490993428 28.43734019767933 21.7870932837941 58-59 22.417894064640603 27.292969359551677 28.44943331701515 21.83970325879257 60-61 22.072858264542607 27.466018430109735 28.88444763402303 21.576675671324633 62-63 21.968144566110215 27.44973309700118 28.92433871256616 21.657783624322448 64-65 21.905941409363695 27.449332670175693 28.687153736190616 21.95757218426999 66-67 21.9272097253525 27.585715662063272 28.464549779190097 22.022524833394133 68-69 21.901929605984225 27.425553342287053 28.649662835671812 22.02285421605691 70-71 22.00344781728133 27.445144337719757 28.555206455249397 21.99620138974952 72-73 22.058724835668862 27.448086183009202 28.493547185115194 21.999641796206745 74-75 21.952163496080875 27.838694363435064 28.15215023812847 22.05699190235559 76-77 22.054886680012153 27.549730606154682 28.269966971153487 22.125415742679678 78-79 22.039579460512147 27.68400661235968 28.174498772019756 22.10191515510842 80-81 22.069743551737414 27.786252627374928 28.075248429751042 22.06875539113661 82-83 22.078135428319452 27.662454817906745 28.273336893415348 21.986072860358462 84-85 22.056677054756307 27.76485614163376 28.07493423552842 22.103532568081512 86-87 22.039318473210027 27.529395392423034 28.194173046407624 22.237113087959315 88-89 22.067226572439562 27.54166560301588 28.040895245357472 22.350212579187083 90-91 21.94923918060346 27.589394271898904 28.201269535150196 22.260097012347437 92-93 22.031994584949025 27.578345724983595 28.141713596833974 22.24794609323341 94-95 21.90608806210181 27.372934461908134 28.123638723213983 22.59733875277608 96-97 21.773964441494638 27.4270767371024 28.310522211508136 22.48843660989482 98-99 21.763103774109275 27.482671376840685 27.963446743948104 22.790778105101932 100-101 21.568131755018012 27.64125579001544 28.02659804426145 22.764014410705094 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 445.0 1 327.0 2 242.5 3 285.0 4 242.5 5 216.0 6 421.5 7 932.5 8 1546.0 9 1911.5 10 1746.5 11 1440.5 12 1433.0 13 1523.0 14 1621.5 15 1797.0 16 1967.0 17 2076.0 18 2211.5 19 2419.5 20 2651.5 21 2995.5 22 3528.5 23 4140.0 24 4964.0 25 6165.5 26 7656.0 27 9597.5 28 11319.5 29 13097.0 30 15334.5 31 17629.5 32 20133.0 33 22493.5 34 25372.5 35 28061.5 36 31230.0 37 34765.0 38 36481.5 39 37277.0 40 38197.0 41 39522.0 42 40906.5 43 42233.5 44 43572.5 45 44809.5 46 44953.5 47 46122.0 48 50663.0 49 50212.5 50 45086.0 51 42138.5 52 39820.0 53 38235.0 54 36419.0 55 33463.0 56 30074.0 57 26772.5 58 24186.5 59 21760.5 60 18638.5 61 14774.0 62 11291.0 63 8580.0 64 6371.0 65 4791.0 66 3518.5 67 2474.0 68 1679.5 69 1104.5 70 753.5 71 543.0 72 389.5 73 282.5 74 211.0 75 148.5 76 92.0 77 52.5 78 29.0 79 15.0 80 10.5 81 8.0 82 6.5 83 3.0 84 1.5 85 1.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020586416423678332 2 0.002882098299314966 3 2.4703699708414E-4 4 0.0 5 0.002882098299314966 6 0.0 7 3.293826627788533E-4 8 0.0 9 8.234566569471333E-5 10-11 0.0 12-13 0.00543481393585108 14-15 0.0024703699708414 16-17 0.0 18-19 3.293826627788533E-4 20-21 9.058023226418467E-4 22-23 0.00135870348396277 24-25 4.1172832847356664E-4 26-27 8.234566569471333E-5 28-29 0.0 30-31 0.0 32-33 1.6469133138942665E-4 34-35 0.0 36-37 0.0016469133138942665 38-39 0.009099196059265824 40-41 6.587653255577066E-4 42-43 0.0 44-45 8.646294897944898E-4 46-47 2.4703699708414E-4 48-49 8.234566569471333E-5 50-51 0.0011528393197259865 52-53 4.5290116132092333E-4 54-55 0.0 56-57 1.6469133138942665E-4 58-59 1.2351849854207E-4 60-61 0.0017704318124363363 62-63 4.1172832847356664E-5 64-65 8.234566569471333E-5 66-67 0.0 68-69 0.0 70-71 1.2351849854207E-4 72-73 4.1172832847356664E-5 74-75 0.0022645058066046164 76-77 0.0 78-79 4.1172832847356664E-5 80-81 0.0012763578182680565 82-83 1.2351849854207E-4 84-85 0.0017704318124363363 86-87 1.6469133138942665E-4 88-89 0.0018116046452836933 90-91 9.469751554892032E-4 92-93 0.0 94-95 0.0 96-97 0.0 98-99 4.1172832847356664E-5 100-101 0.00148222198250484 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1214393.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.08723178933458 #Duplication Level Percentage of deduplicated Percentage of total 1 83.02365407948452 54.037798170689435 2 10.519995729530365 13.694348009415059 3 2.855319654777505 5.575345566094389 4 1.1627282215145236 3.0271504504686626 5 0.6457874181898063 2.1016257687177933 6 0.35223923192839307 1.3755765920292304 7 0.2540862764175769 1.1576440657375835 8 0.18417997153574298 0.9590211598639958 9 0.13435446297712694 0.7870284066351448 >10 0.7792778491129352 9.46102902263759 >50 0.05907830105574513 2.628788880437733 >100 0.027396292154390905 3.127604425458695 >500 0.001141506792898353 0.48730916015628695 >1k 5.07336352399268E-4 0.6562121826329534 >5k 2.53668176199634E-4 0.923518139025645 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6016 0.49539152481939536 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5171 0.42580943730736265 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3169 0.26095341458654653 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2279 0.18766577211825167 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1431 0.11783664760913477 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 8.234566569471333E-5 0.0 14-15 0.0 0.0 0.0 8.234566569471333E-5 0.0 16-17 0.0 0.0 0.0 1.6469133138942665E-4 0.0 18-19 0.0 0.0 0.0 2.0586416423678332E-4 0.0 20-21 0.0 0.0 0.0 4.5290116132092333E-4 0.0 22-23 0.0 0.0 0.0 0.0010293208211839165 0.0 24-25 0.0 0.0 0.0 0.0013175306511154132 0.0 26-27 0.0 0.0 0.0 0.0015233948153521965 0.0 28-29 4.1172832847356664E-5 0.0 0.0 0.0024291971379940434 0.0 30-31 8.234566569471333E-5 0.0 0.0 0.004405493114667163 0.0 32-33 8.234566569471333E-5 0.0 0.0 0.006134752094256143 0.0 34-35 8.234566569471333E-5 0.0 0.0 0.008563949232250186 0.0 36-37 8.234566569471333E-5 0.0 0.0 0.011446047531565152 0.0 38-39 8.234566569471333E-5 0.0 0.0 0.013051788012612062 0.0 40-41 8.234566569471333E-5 0.0 0.0 0.015563330816300819 0.0 42-43 8.234566569471333E-5 0.0 0.0 0.020586416423678332 0.0 44-45 8.234566569471333E-5 0.0 0.0 0.027009378347865973 0.0 46-47 8.234566569471333E-5 0.0 0.0 0.04236684499993001 0.0 48-49 8.234566569471333E-5 0.0 0.0 0.07612856793476247 0.0 50-51 8.234566569471333E-5 0.0 0.0 0.09692084852267759 0.0 52-53 8.234566569471333E-5 0.0 0.0 0.11478985797843037 0.0 54-55 8.234566569471333E-5 0.0 0.0 0.13858775536420254 0.0 56-57 8.234566569471333E-5 0.0 0.0 0.15802133246815486 0.0 58-59 8.234566569471333E-5 0.0 0.0 0.1812839830269114 0.0 60-61 8.234566569471333E-5 0.0 0.0 0.218874779416548 0.0 62-63 8.234566569471333E-5 0.0 0.0 0.2495073670549814 0.0 64-65 8.234566569471333E-5 0.0 0.0 0.2827338431627982 0.0 66-67 8.234566569471333E-5 0.0 0.0 0.34515185775939095 0.0 68-69 8.234566569471333E-5 0.0 0.0 0.4174101794065018 0.0 70-71 8.234566569471333E-5 0.0 0.0 0.47608146621398506 0.0 72-73 8.234566569471333E-5 0.0 0.0 0.5416286161069769 0.0 74-75 8.234566569471333E-5 0.0 0.0 0.6003822485801549 0.0 76-77 8.234566569471333E-5 0.0 0.0 0.6479780433516992 0.0 78-79 8.234566569471333E-5 0.0 0.0 0.7039730960241042 0.0 80-81 8.234566569471333E-5 0.0 0.0 0.8072345608052747 0.0 82-83 8.234566569471333E-5 0.0 0.0 0.9240418875932257 0.0 84-85 8.234566569471333E-5 0.0 0.0 1.046901620809738 0.0 86-87 8.234566569471333E-5 0.0 0.0 1.2506659705713061 0.0 88-89 8.234566569471333E-5 0.0 0.0 1.4965501283357199 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7940 0.0 28.782936 1 GGTATCA 5570 0.0 28.661217 1 ATCAACG 9515 0.0 23.811947 3 TCAACGC 9625 0.0 23.53981 4 TATCAAC 9745 0.0 23.397131 2 CAACGCA 9715 0.0 23.272844 5 AACGCAG 10055 0.0 22.442354 6 TACACCG 110 0.0028777777 21.590496 5 GTACATG 5200 0.0 21.380754 1 GAGTACT 5625 0.0 20.98534 12-13 TACATGG 5235 0.0 20.869612 2 ACATGGG 5080 0.0 20.476973 3 AGAGTAC 8480 0.0 20.280075 10-11 ACGCAGA 11270 0.0 19.980726 7 AGTACTT 5890 0.0 19.96053 12-13 GTACTTT 5940 0.0 19.750494 14-15 CGCAGAG 11245 0.0 19.729418 8 GTGTAGC 695 0.0 19.141842 1 TAAGGTG 455 0.0 18.79085 5 GCAGAGT 11765 0.0 18.413221 9 >>END_MODULE