FastQCFastQC Report
Thu 26 May 2016
SRR936871_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936871_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2278309
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83250.36540258586521845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60210.2642749512906283No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39150.17183797281229193No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34420.15107696102679663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA121150.028.4072151
GGTATCA85650.026.695081
GTACATG84000.025.0690561
ATCAACG142700.023.9121443
TCAACGC143300.023.8120234
CAACGCA144550.023.6353475
TATCAAC145700.023.4575612
TACATGG86850.023.2612292
AACGCAG149300.022.9783616
GAGTACT74550.022.11734812-13
ACATGGG87950.021.7224223
AGAGTAC121150.020.80786310-11
ACGCAGA163600.020.7084667
CGCAGAG163250.020.5222688
AGTACTT79750.020.49646412-13
GTACTTT81450.019.95177314-15
GCAGAGT171300.018.9206269
CATGGGG41200.017.6486824
ACGTTAT1350.00937877117.6005278
GTGTAGC12700.017.5916941
Produced by FastQC (version 0.11.5)