##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936871_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2278309 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.58788118731919 33.0 31.0 34.0 30.0 34.0 2 31.686195331713126 33.0 31.0 34.0 30.0 34.0 3 31.69915450450312 33.0 31.0 34.0 30.0 34.0 4 35.26521907256654 37.0 35.0 37.0 33.0 37.0 5 35.29513380318473 37.0 35.0 37.0 33.0 37.0 6 35.342258666405655 37.0 35.0 37.0 33.0 37.0 7 35.273943964580745 37.0 35.0 37.0 33.0 37.0 8 35.24254787212797 37.0 35.0 37.0 32.0 37.0 9 36.839380874148326 39.0 37.0 39.0 33.0 39.0 10-11 36.70014317636458 39.0 37.0 39.0 32.0 39.0 12-13 36.5855364658613 39.0 37.0 39.0 32.0 39.0 14-15 37.70467394896829 40.0 37.0 41.0 32.0 41.0 16-17 37.63109328014769 40.0 37.0 41.0 32.0 41.0 18-19 37.58087182203994 40.0 37.0 41.0 32.0 41.0 20-21 37.51608100569325 40.0 37.0 41.0 32.0 41.0 22-23 37.374334429614244 40.0 37.0 41.0 32.0 41.0 24-25 37.19685148063761 39.5 37.0 41.0 31.0 41.0 26-27 37.15698177902998 39.5 36.5 41.0 31.0 41.0 28-29 37.234629060412786 40.0 37.0 41.0 31.0 41.0 30-31 37.11104749180203 39.5 36.5 41.0 31.0 41.0 32-33 36.93722668874152 39.0 36.0 41.0 30.5 41.0 34-35 36.74367546281036 39.0 36.0 41.0 30.0 41.0 36-37 36.589890352888915 39.0 36.0 41.0 30.0 41.0 38-39 36.428906702295436 39.0 35.0 41.0 30.0 41.0 40-41 36.23089975942683 39.0 35.0 41.0 30.0 41.0 42-43 36.03204350243975 39.0 35.0 40.5 29.0 41.0 44-45 35.79536840700713 39.0 35.0 40.0 28.0 41.0 46-47 35.4759220544711 38.5 35.0 40.0 27.0 41.0 48-49 35.157964086522064 38.0 34.5 40.0 26.0 41.0 50-51 34.51635247896576 37.5 33.5 39.5 24.5 40.5 52-53 34.83171817343477 38.0 34.5 40.0 26.0 40.5 54-55 35.146823148220896 39.0 35.0 40.0 26.0 41.0 56-57 35.03804466382743 39.0 34.5 40.5 26.0 41.0 58-59 34.79771554253615 38.0 34.0 40.0 25.0 41.0 60-61 34.51096185811494 38.0 34.0 40.0 24.5 41.0 62-63 34.20800909797573 38.0 34.0 40.0 23.5 41.0 64-65 33.902617028682236 37.0 33.5 40.0 23.0 41.0 66-67 33.535099497039255 37.0 33.0 39.5 22.0 41.0 68-69 33.09394006695317 36.0 32.5 39.0 21.5 41.0 70-71 32.7381072980004 36.0 32.0 39.0 20.5 40.5 72-73 32.35177032614979 35.0 32.0 38.5 20.0 40.0 74-75 31.913742385251517 35.0 31.5 37.5 18.5 39.5 76-77 31.47082309730594 35.0 31.0 37.0 17.5 39.0 78-79 31.028528834324053 35.0 31.0 36.5 13.0 39.0 80-81 30.596441044651975 35.0 30.5 36.0 9.0 38.0 82-83 30.17197491648411 34.0 30.0 35.5 7.0 37.0 84-85 29.812372026797068 34.0 30.0 35.0 4.5 37.0 86-87 29.479879814371095 34.0 29.5 35.0 2.0 36.0 88-89 29.101258213877045 34.0 29.0 35.0 2.0 36.0 90-91 28.763955854978406 34.0 29.0 35.0 2.0 36.0 92-93 28.400314224277743 34.0 29.0 35.0 2.0 35.0 94-95 27.989788479086904 34.0 28.5 35.0 2.0 35.0 96-97 27.543968574938695 34.0 27.0 35.0 2.0 35.0 98-99 26.933401044371067 34.0 26.5 35.0 2.0 35.0 100-101 24.78212283759578 31.5 21.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5421816459812412 1101 2 0.520341909438347 1101 3 0.523433368811741 1101 4 0.5040905384663503 1101 5 0.4397008390151669 1101 6 0.41334144694780406 1101 7 0.5087442581096155 1101 8 0.4708081962027606 1101 9 0.5422280862354754 1101 10-11 0.5297517801595149 1101 12-13 0.6399762378176561 1101 14-15 0.9580364115325111 1101 16-17 0.848024159883245 1101 18-19 0.9031783277136114 1101 20-21 0.8805645450300759 1101 22-23 0.8258689673111022 1101 24-25 0.9541121490324613 1101 26-27 0.9308147628333856 1101 28-29 1.014393080080687 1101 30-31 0.894580720272586 1101 32-33 0.9456220037873564 1101 34-35 1.0409702220305093 1101 36-37 1.0068924763494493 1101 38-39 1.0219696012135486 1101 40-41 1.1047779255780483 1101 42-43 1.0255621525874474 1101 44-45 0.9994818031576642 1101 46-47 0.963276171644651 1101 48-49 1.1204751009079956 1101 50-51 1.0466201460091327 1101 52-53 1.1033204656896842 1101 54-55 1.0565030028036375 1101 56-57 1.1712083496669408 1101 58-59 1.1325463990658875 1101 60-61 1.021224556615003 1101 62-63 1.0482816347542538 1101 64-65 0.9381942612624883 1101 66-67 0.9862816895638318 1101 68-69 1.0425154727477661 1101 70-71 1.0480522763518039 1101 72-73 0.9317853339666371 1101 74-75 0.8793527607314182 1101 76-77 1.0618603428734552 1101 78-79 1.0732499845255035 1101 80-81 0.9878476370702636 1101 82-83 1.0114039720286279 1101 84-85 1.2709649087446557 1101 86-87 1.1746477288318005 1101 88-89 1.2330978621855593 1101 90-91 1.2641766710619748 1101 92-93 1.2530011827963463 1101 94-95 1.3155166905023137 1101 96-97 1.2583442048529179 1101 98-99 1.5157152146286883 1101 100-101 1.91682839119116 1102 1 0.3707893538367344 1102 2 0.47461130435201326 1102 3 0.40818526559192136 1102 4 0.3710378405992216 1102 5 0.3278418851365146 1102 6 0.3720551592560213 1102 7 0.2800519266170127 1102 8 0.2892305896434024 1102 9 0.4621473469620341 1102 10-11 0.5318089374779262 1102 12-13 0.5191061067245855 1102 14-15 0.6489735899374622 1102 16-17 0.5257392628272939 1102 18-19 0.5396146048116748 1102 20-21 0.6098824104124887 1102 22-23 0.5788933269796388 1102 24-25 0.6463906730415303 1102 26-27 0.594450939207043 1102 28-29 0.6316927705086712 1102 30-31 0.5779001948913702 1102 32-33 0.6059287255862031 1102 34-35 0.4575079003581166 1102 36-37 0.44147195194354083 1102 38-39 0.41645416212911357 1102 40-41 0.5448246220192274 1102 42-43 0.5435570768507674 1102 44-45 0.5266233739203514 1102 46-47 0.45155371699584634 1102 48-49 0.5441982214477079 1102 50-51 0.3284411348571368 1102 52-53 0.40905646012041785 1102 54-55 0.5967920305692758 1102 56-57 0.4543838211715183 1102 58-59 0.31597265164705135 1102 60-61 0.42075794953100143 1102 62-63 0.45660560749980306 1102 64-65 0.5803998063177502 1102 66-67 0.41319049277625197 1102 68-69 0.2892618977879522 1102 70-71 0.4447408142840388 1102 72-73 0.38939090962597334 1102 74-75 0.37991691831214425 1102 76-77 0.3252542509805174 1102 78-79 0.3979757459280435 1102 80-81 0.618724733954032 1102 82-83 0.5124222512387071 1102 84-85 0.6001689336852927 1102 86-87 0.8109650367828038 1102 88-89 0.77690982703459 1102 90-91 0.5161512225945337 1102 92-93 0.3716103746132866 1102 94-95 0.5435532203362428 1102 96-97 0.4433308239767655 1102 98-99 0.36972766981557115 1102 100-101 0.7032089882060859 1103 1 0.28801354256023615 1103 2 0.4011253728968036 1103 3 0.3229917052558413 1103 4 0.3290307879396295 1103 5 0.30271494662463994 1103 6 0.2945788720520426 1103 7 0.41867399686812945 1103 8 0.33836251342546575 1103 9 0.4539968046400631 1103 10-11 0.5226099906964876 1103 12-13 0.3221137170176718 1103 14-15 0.4560624890191818 1103 16-17 0.572272908720663 1103 18-19 0.6732741381379626 1103 20-21 0.5431990726885161 1103 22-23 0.49311003913511087 1103 24-25 0.6728528562635816 1103 26-27 0.6430847371458057 1103 28-29 0.6742032980139143 1103 30-31 0.6991955092990452 1103 32-33 0.7608258040948428 1103 34-35 0.6572984869163463 1103 36-37 0.6873014016782903 1103 38-39 0.5097902412836461 1103 40-41 0.593245797114804 1103 42-43 0.613778111000137 1103 44-45 0.6937242012772842 1103 46-47 0.5910556981325925 1103 48-49 0.5463445086383203 1103 50-51 0.5741321791562441 1103 52-53 0.5703944060767796 1103 54-55 0.5214954618205496 1103 56-57 0.6391256582596299 1103 58-59 0.6730432972582676 1103 60-61 0.6489136268952294 1103 62-63 0.6805141770027348 1103 64-65 0.6983419648809033 1103 66-67 0.7688550193962342 1103 68-69 0.780082341140016 1103 70-71 0.7136826345227973 1103 72-73 0.7786371415881277 1103 74-75 0.86185467464637 1103 76-77 0.7130616013120878 1103 78-79 0.7253019267537439 1103 80-81 0.8418931352575783 1103 82-83 0.8238431735939251 1103 84-85 0.9672107882913856 1103 86-87 0.9596693078274505 1103 88-89 0.9135106430248996 1103 90-91 0.9650147601839763 1103 92-93 0.816441433060497 1103 94-95 0.9161119022350874 1103 96-97 0.8866434343835188 1103 98-99 1.0788014499421585 1103 100-101 1.5050317944799332 1104 1 0.4268197333336907 1104 2 0.48633219012630136 1104 3 0.45904676693024626 1104 4 0.4730790279866426 1104 5 0.4546794018412186 1104 6 0.32567811426989834 1104 7 0.4428819783187805 1104 8 0.5219820562151725 1104 9 0.45171478458809133 1104 10-11 0.48897218441653223 1104 12-13 0.5282425534195525 1104 14-15 0.7943560804276402 1104 16-17 0.7658213155212792 1104 18-19 0.7275331962793885 1104 20-21 0.750080438186771 1104 22-23 0.8370060630694454 1104 24-25 0.7229186414776052 1104 26-27 0.7979670750680228 1104 28-29 0.827523771569723 1104 30-31 0.7572758515531888 1104 32-33 0.7913356059084933 1104 34-35 0.7096524003730522 1104 36-37 0.8115285628477906 1104 38-39 0.8006738888019598 1104 40-41 0.7879651440759261 1104 42-43 0.8950297525763844 1104 44-45 0.7606852420152492 1104 46-47 0.7537882827646314 1104 48-49 0.8750317485057479 1104 50-51 0.8842780061014253 1104 52-53 0.8744308206665536 1104 54-55 0.9644590093599845 1104 56-57 1.0912545459703153 1104 58-59 1.0264479416821572 1104 60-61 0.882091364250357 1104 62-63 0.9679479184039295 1104 64-65 0.9460187145415873 1104 66-67 0.7528442298881473 1104 68-69 0.876033465841175 1104 70-71 0.8959143963815492 1104 72-73 0.8702874892052606 1104 74-75 0.7119822747504507 1104 76-77 0.7513581246520964 1104 78-79 1.0276561456199858 1104 80-81 1.1439126252725202 1104 82-83 1.0722052622530924 1104 84-85 1.197854237317923 1104 86-87 1.0558971335681022 1104 88-89 1.113102493721172 1104 90-91 0.9994997725164154 1104 92-93 1.0387716345208418 1104 94-95 1.1382625228883434 1104 96-97 0.9216806271567961 1104 98-99 0.9346957929024065 1104 100-101 1.2738141755259704 1105 1 0.32194033608335815 1105 2 0.32039591572270965 1105 3 0.3428805408463056 1105 4 0.43474155967609107 1105 5 0.43071002008350945 1105 6 0.3186708852824438 1105 7 0.3330213103484212 1105 8 0.35448602416819597 1105 9 0.30824585921269687 1105 10-11 0.4792620413535431 1105 12-13 0.4158440206142018 1105 14-15 0.44853314660714005 1105 16-17 0.6736176153035132 1105 18-19 0.5832309838408705 1105 20-21 0.5897867317936303 1105 22-23 0.5244341185287027 1105 24-25 0.718905574994757 1105 26-27 0.5463728686857081 1105 28-29 0.3895188901749478 1105 30-31 0.40318183476590264 1105 32-33 0.4289966601110038 1105 34-35 0.3009705902551758 1105 36-37 0.3821533021546344 1105 38-39 0.4640202443792987 1105 40-41 0.7013816667406587 1105 42-43 0.48349555301950176 1105 44-45 0.5568634802982757 1105 46-47 0.4876649186988473 1105 48-49 0.5308154632410691 1105 50-51 0.3934233421992346 1105 52-53 0.4411758024925163 1105 54-55 0.5559936253488047 1105 56-57 0.7166963700911424 1105 58-59 0.5837995903463238 1105 60-61 0.39412260747911176 1105 62-63 0.44920771978959806 1105 64-65 0.5954507420166166 1105 66-67 0.6887622963980817 1105 68-69 0.7481965656385725 1105 70-71 0.739280551096499 1105 72-73 0.726291421947785 1105 74-75 0.7340267591603258 1105 76-77 0.6302028409096003 1105 78-79 0.5094980206606188 1105 80-81 0.5089407733624896 1105 82-83 0.5735572271346783 1105 84-85 0.6153869187603647 1105 86-87 0.698346054636982 1105 88-89 0.6438708885013504 1105 90-91 0.5715307313526274 1105 92-93 0.7096887564412775 1105 94-95 0.6380784295046666 1105 96-97 0.899908177751584 1105 98-99 0.8020252352492676 1105 100-101 0.9636941855745071 1106 1 0.5786624364732269 1106 2 0.6001961106544833 1106 3 0.5656631919184285 1106 4 0.42194916201527377 1106 5 0.44248207174042875 1106 6 0.49329929658848926 1106 7 0.5139819584965721 1106 8 0.5084394842071873 1106 9 0.6147577987838488 1106 10-11 0.6591810228350212 1106 12-13 0.6690634406921774 1106 14-15 0.8504718040145534 1106 16-17 0.8878735241729103 1106 18-19 0.8997803868623109 1106 20-21 0.8648065295519061 1106 22-23 0.9698867257411763 1106 24-25 0.9481316349362743 1106 26-27 0.970180434475175 1106 28-29 0.8631406616174431 1106 30-31 0.8888558722902715 1106 32-33 0.9489802127425762 1106 34-35 0.8861816648165828 1106 36-37 0.9051598201858226 1106 38-39 0.9689620694280308 1106 40-41 1.024015073173402 1106 42-43 0.9953330895692076 1106 44-45 0.9664455953080235 1106 46-47 0.6097348504727265 1106 48-49 0.6882335309798648 1106 50-51 0.7149952842070277 1106 52-53 0.8351405320246883 1106 54-55 0.803011778370248 1106 56-57 0.8554451517675545 1106 58-59 0.9875733476562729 1106 60-61 0.9314840231711159 1106 62-63 0.821958389867568 1106 64-65 0.6941471357787989 1106 66-67 0.9039882954234244 1106 68-69 0.928336368270152 1106 70-71 1.0187715964181407 1106 72-73 0.8543737806222467 1106 74-75 0.9563220807977579 1106 76-77 1.0669840299934172 1106 78-79 1.0644139563331336 1106 80-81 1.1142313435379236 1106 82-83 1.246901829961189 1106 84-85 1.3464313266551002 1106 86-87 1.333331739334838 1106 88-89 1.3127274918151883 1106 90-91 1.526393365368218 1106 92-93 1.4747518046868962 1106 94-95 1.5276815605962852 1106 96-97 1.4356020130342344 1106 98-99 1.62106001838767 1106 100-101 1.6358478770949745 1107 1 0.2544037091369411 1107 2 0.3256898491611473 1107 3 0.3567173872709368 1107 4 0.3971805485105122 1107 5 0.260540666855853 1107 6 0.23481056575671033 1107 7 0.332575706121915 1107 8 0.3692255523845134 1107 9 0.47328072466417836 1107 10-11 0.5933463406970034 1107 12-13 0.4084469229639396 1107 14-15 0.3468344007105415 1107 16-17 0.5993030291805823 1107 18-19 0.42305448677264934 1107 20-21 0.4976287418955607 1107 22-23 0.5771374058426986 1107 24-25 0.47677198281390787 1107 26-27 0.43597598227986367 1107 28-29 0.4571472194678705 1107 30-31 0.5184230009700386 1107 32-33 0.41632992550417214 1107 34-35 0.5476617974933689 1107 36-37 0.6251074067752853 1107 38-39 0.7405681158724988 1107 40-41 0.7567015501715275 1107 42-43 0.900937373068956 1107 44-45 0.9984699231853469 1107 46-47 1.0706127703499249 1107 48-49 1.0880570465348143 1107 50-51 0.7957034826598957 1107 52-53 0.8133708242783655 1107 54-55 0.9404400637545223 1107 56-57 0.9356802237291362 1107 58-59 1.0253309503553965 1107 60-61 0.9654698170281648 1107 62-63 0.8639116300383947 1107 64-65 1.0480361576484327 1107 66-67 1.0030455569319585 1107 68-69 1.008273278405568 1107 70-71 0.9879718312241366 1107 72-73 0.9325105114236294 1107 74-75 0.9115989467075671 1107 76-77 0.9287350634437708 1107 78-79 0.952798468255807 1107 80-81 1.0587652650260964 1107 82-83 1.0008475586196788 1107 84-85 0.9647145642422963 1107 86-87 1.1218916908538574 1107 88-89 1.093697472362006 1107 90-91 0.9185853059752205 1107 92-93 1.039573648483909 1107 94-95 0.9578382689049221 1107 96-97 0.931582860007591 1107 98-99 1.1184957696908846 1107 100-101 1.3679607321535627 1108 1 -0.0033011839519367925 1108 2 0.1259056219409196 1108 3 0.19425731858947515 1108 4 0.24150866234400326 1108 5 0.13908244430233907 1108 6 0.11497363364744473 1108 7 0.07125787346479484 1108 8 -0.11708922497536634 1108 9 -0.10583172565183219 1108 10-11 -0.07660272689103209 1108 12-13 -0.21001419463546256 1108 14-15 0.022773469585445127 1108 16-17 0.008506879421652513 1108 18-19 0.23391637195983606 1108 20-21 0.30945780064548956 1108 22-23 0.1603398267711995 1108 24-25 0.36946217405527904 1108 26-27 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-2.3940874953399387 2316 96-97 -2.5767650320640705 2316 98-99 -2.789010965534583 2316 100-101 -2.49892724718287 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6761.0 3 2275.0 4 2329.0 5 2399.0 6 2578.0 7 3137.0 8 3679.0 9 4560.0 10 5633.0 11 6980.0 12 8442.0 13 10300.0 14 11418.0 15 13249.0 16 16007.0 17 20160.0 18 18090.0 19 13353.0 20 13030.0 21 13999.0 22 15151.0 23 17073.0 24 19447.0 25 22068.0 26 26093.0 27 30579.0 28 36206.0 29 44530.0 30 53560.0 31 66270.0 32 83453.0 33 108229.0 34 145007.0 35 203861.0 36 302016.0 37 442583.0 38 407779.0 39 75997.0 40 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.5716133407057 19.06686558590338 14.527369913802152 22.83415115958877 2 15.033456497765908 20.513775817300722 40.76183279220066 23.690934892732713 3 19.10423466360266 25.007499702427623 29.8748175584834 26.013448075486316 4 12.49408879552067 17.04329902344265 38.21868380110225 32.24392837993443 5 13.674634580588238 37.405256345023766 34.35100651265138 14.569102561736614 6 31.011000569866166 36.72679772563207 18.007111683662327 14.25509002083944 7 27.804274653519297 32.949502139963265 21.308892931529964 17.937330274987477 8 25.70370972454411 34.939599173127064 20.202148903031354 19.154542199297467 9 25.949475560430518 17.305978452890088 20.875981950911683 35.86856403576771 10-11 24.3538389137472 27.013116781139885 27.904808314662137 20.728235990450774 12-13 25.59186444907559 24.3780736059638 28.16084821035322 21.869213734607396 14-15 22.481048440598823 25.68407506636451 26.725125721310366 25.109750771726304 16-17 21.82253448467541 29.029613032529035 27.10198218498665 22.045870297808914 18-19 21.61015469229034 27.71263957023647 29.451810308147884 21.225395429325307 20-21 22.527706758272494 26.76121113141704 29.405479438215153 21.30560267209531 22-23 22.11770845138353 26.774991720014373 29.30192473427176 21.80537509433034 24-25 22.13215988278378 26.91321836000945 29.313739613794336 21.64088214341243 26-27 21.92191869077488 27.027669168045303 29.328668153118276 21.721743988061544 28-29 21.626650216024487 27.258615998220236 29.39470318896636 21.72003059678892 30-31 21.858109955200312 26.96199810595456 29.34826260964154 21.831629329203587 32-33 21.74715349238571 27.065706541011558 29.323919446936003 21.86322051966673 34-35 22.232583200460557 27.030977397649174 29.057816015081407 21.678623386808862 36-37 22.140621092263586 26.815665396868475 29.153253421461677 21.890460089406265 38-39 21.89066688448796 27.170944922831534 29.182695854720226 21.755692337960276 40-41 22.147730007888445 27.05725446997208 29.08097426680511 21.71404125533436 42-43 21.920976510447364 27.095556037704853 28.82995394498169 22.15351350686609 44-45 22.09487431377707 27.19790052341996 28.682131393823568 22.02509376897941 46-47 22.225910557860765 27.39491536586972 28.34670355002305 22.03247052624646 48-49 22.28343348257649 27.443454174036606 28.188096438363864 22.085015905023038 50-51 22.01864850477189 27.56839522975858 28.236690027605054 22.17626623786448 52-53 22.36835865252375 27.744339408885168 27.849484746131864 22.03781719245922 54-55 22.10515221711111 27.551900929880226 27.95656899447781 22.386377858530857 56-57 22.210208851858233 27.807017062328388 27.844630817588655 22.138143268224724 58-59 22.49364780687664 27.476213449499433 27.902152400372515 22.12798634325141 60-61 22.35760950142465 27.407656755588818 28.326807340029724 21.90792640295681 62-63 22.141040731533778 27.567680928998712 28.247784229157702 22.043494110309815 64-65 22.03426782864373 27.662778114064064 28.058110270139245 22.244843787152956 66-67 22.12466048721183 27.76915941377166 27.725062280612374 22.38111781840414 68-69 21.969422489704804 27.62480824827747 28.030248134304596 22.375521127713125 70-71 22.165847126487247 27.588861570882905 27.942090705536287 22.303200597093557 72-73 22.19007666714885 27.502032084434617 27.967079244378485 22.340812004038046 74-75 22.020634960359434 27.8822887867049 27.740428373663228 22.356647879272444 76-77 22.1611600169852 27.677221523775984 27.75852476444455 22.403093694794265 78-79 22.200940041861138 27.685535069077904 27.735539164714197 22.377985724346768 80-81 22.170695947212817 27.84410181740261 27.647888727835735 22.337313507548835 82-83 22.184798142742828 27.797853754173424 27.73417084411917 22.283177258964574 84-85 22.195291208513684 27.741725795577576 27.608864175895608 22.454118820013132 86-87 22.205814952200896 27.689090919871738 27.786904992756178 22.31818913517119 88-89 22.16140308640701 27.67815681920923 27.509193893962802 22.651246200420957 90-91 22.0969301840203 27.656541114030396 27.78331914684291 22.46320955510639 92-93 22.309266893472348 27.607932195498 27.800065450056994 22.28273546097266 94-95 22.128879596359223 27.57093991975419 27.707318701772397 22.59286178211419 96-97 22.016481063954295 27.66333448730948 27.790784416752924 22.5294000319833 98-99 21.974402909611236 27.684484197490388 27.51981658480162 22.82129630809675 100-101 21.97141115666252 27.54172245847529 27.70730712660064 22.779559258261546 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 488.0 1 356.5 2 258.5 3 325.5 4 337.5 5 354.5 6 506.5 7 985.5 8 1748.0 9 2208.0 10 2101.5 11 1980.0 12 2125.5 13 2313.0 14 2518.5 15 2726.5 16 2858.5 17 3010.0 18 3189.0 19 3466.0 20 3921.0 21 4653.5 22 5640.0 23 6867.0 24 8505.0 25 10631.5 26 13638.0 27 17024.5 28 19950.5 29 23290.0 30 27746.5 31 32607.5 32 37629.0 33 42917.0 34 48438.5 35 52580.0 36 57473.0 37 63189.5 38 66408.0 39 68495.0 40 70638.5 41 74101.5 42 77202.0 43 79952.0 44 83609.0 45 86035.0 46 85709.5 47 87472.0 48 94719.0 49 94885.5 50 86862.0 51 80884.5 52 76571.5 53 73492.5 54 69742.0 55 64120.0 56 58394.5 57 52231.0 58 46547.5 59 41114.0 60 34939.5 61 28006.5 62 21433.5 63 16544.5 64 12797.0 65 9683.0 66 7186.5 67 5550.5 68 3971.0 69 2537.5 70 1763.0 71 1252.0 72 924.0 73 690.5 74 481.0 75 352.0 76 246.0 77 137.0 78 80.5 79 53.0 80 38.0 81 31.0 82 23.5 83 21.5 84 18.5 85 9.0 86 6.0 87 6.0 88 5.5 89 3.5 90 2.5 91 3.5 92 3.5 93 3.0 94 1.5 95 0.5 96 0.0 97 0.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.058727766953472954 2 0.09739679736155192 3 0.06812069828982811 4 0.13031594924130133 5 0.10670194429289442 6 0.10253218505479283 7 0.11275906823876831 8 0.09937194647433689 9 0.1201329582598322 10-11 0.11069613472097067 12-13 0.09548748655252647 14-15 0.0984502102217039 16-17 0.11359302008638864 18-19 0.09131772731442486 20-21 0.0765260550697908 22-23 0.07615297134848696 24-25 0.06544327393694183 26-27 0.05697207885321965 28-29 0.07020557790887891 30-31 0.05146360743867491 32-33 0.05163917624870024 34-35 0.04687687227676316 36-37 0.05159528404619391 38-39 0.053241241640181375 40-41 0.0685157281123851 42-43 0.06438986107678984 44-45 0.05135387693240908 46-47 0.03770340195293966 48-49 0.04569178280909218 50-51 0.02837630892034399 52-53 0.043233819468737555 54-55 0.0497737576421811 56-57 0.05339486434895355 58-59 0.05879360525723245 60-61 0.05534806736048534 62-63 0.06359980143167586 64-65 0.04316798116497806 66-67 0.06658447120210648 68-69 0.056006450398080325 70-71 0.02673035132635652 72-73 0.020870742291761127 74-75 0.03151460139954677 76-77 0.04529675298653519 78-79 0.06564078884822033 80-81 0.04117088595093993 82-83 0.06158076011638456 84-85 0.06601387256952415 86-87 0.06761593796100529 88-89 0.09085685918810837 90-91 0.06561884274696715 92-93 0.07742584522117062 94-95 0.08464611253346231 96-97 0.09309536151593134 98-99 0.12360044225783245 100-101 0.12008906605732585 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2278309.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.616870156895054 #Duplication Level Percentage of deduplicated Percentage of total 1 78.4532246027796 46.77135704533636 2 12.765426941140632 15.220696008946218 3 3.972908921035936 7.105571858717082 4 1.6903221350071846 4.030868609841957 5 0.8517832948164276 2.5390327044441605 6 0.49937105867240134 1.786256373899026 7 0.3375746108687321 1.40875992210979 8 0.23078264925685552 1.1006831388168166 9 0.17496889348101696 0.938798802373805 >10 0.9074475858786861 10.064351594678339 >50 0.07598018280839715 3.1126635841396784 >100 0.03792479016384524 3.9764164374536697 >500 0.001621140322114778 0.658080334137503 >1k 5.158173752182429E-4 0.6562352490884236 >5k 1.4737639291949797E-4 0.6302283360170388 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8325 0.36540258586521845 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6021 0.2642749512906283 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3915 0.17183797281229193 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3442 0.15107696102679663 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.389220250633255E-5 0.0 10-11 0.0 0.0 0.0 4.389220250633255E-5 0.0 12-13 0.0 0.0 0.0 4.389220250633255E-5 4.389220250633255E-5 14-15 0.0 0.0 0.0 4.389220250633255E-5 1.3167660751899765E-4 16-17 0.0 0.0 0.0 6.583830375949883E-5 2.633532150379953E-4 18-19 0.0 0.0 0.0 8.77844050126651E-5 2.633532150379953E-4 20-21 0.0 0.0 0.0 1.3167660751899765E-4 2.633532150379953E-4 22-23 0.0 0.0 0.0 3.7308372130382664E-4 2.633532150379953E-4 24-25 0.0 0.0 0.0 7.461674426076533E-4 2.633532150379953E-4 26-27 0.0 0.0 0.0 0.0010314667588988149 2.633532150379953E-4 28-29 0.0 0.0 0.0 0.0020409874165444636 2.633532150379953E-4 30-31 0.0 0.0 0.0 0.003774729415544599 2.633532150379953E-4 32-33 0.0 0.0 0.0 0.00577182462958273 2.633532150379953E-4 34-35 0.0 0.0 0.0 0.007746973742367694 3.072454175443278E-4 36-37 0.0 0.0 0.0 0.011236403841621133 3.072454175443278E-4 38-39 0.0 0.0 0.0 0.015208648168444227 3.291915187974941E-4 40-41 0.0 0.0 0.0 0.023635951049660077 3.511376200506604E-4 42-43 0.0 0.0 0.0 0.030548972944407456 3.7308372130382664E-4 44-45 0.0 0.0 0.0 0.03858124600306631 3.950298225569929E-4 46-47 0.0 0.0 0.0 0.05352654095647254 3.950298225569929E-4 48-49 0.0 0.0 0.0 0.06401677735548603 3.950298225569929E-4 50-51 0.0 0.0 0.0 0.07674551608232247 3.950298225569929E-4 52-53 0.0 0.0 0.0 0.08945230870790573 3.950298225569929E-4 54-55 0.0 0.0 0.0 0.10593383074903359 4.3892202506332545E-4 56-57 0.0 0.0 0.0 0.15197675117817644 5.047603288228244E-4 58-59 0.0 0.0 0.0 0.1930598527241037 5.267064300759906E-4 60-61 0.0 0.0 0.0 0.22332352635222 5.267064300759906E-4 62-63 0.0 0.0 0.0 0.2635068377467674 5.267064300759906E-4 64-65 0.0 0.0 0.0 0.3068284416205177 5.267064300759906E-4 66-67 0.0 0.0 0.0 0.37257896097500387 5.267064300759906E-4 68-69 0.0 0.0 0.0 0.4532967213841494 5.267064300759906E-4 70-71 0.0 0.0 0.0 0.4956746429040134 5.705986325823231E-4 72-73 0.0 0.0 0.0 0.5426392995857893 6.583830375949882E-4 74-75 0.0 0.0 0.0 0.6349665475578599 6.583830375949882E-4 76-77 0.0 0.0 0.0 0.7406370250918555 6.583830375949882E-4 78-79 0.0 0.0 0.0 0.844069000298028 6.583830375949882E-4 80-81 0.0 0.0 2.1946101253166275E-5 0.920353648254034 6.803291388481545E-4 82-83 0.0 0.0 4.389220250633255E-5 1.0292721487734982 7.022752401013208E-4 84-85 0.0 0.0 4.389220250633255E-5 1.2411837024740717 7.022752401013208E-4 86-87 0.0 0.0 4.389220250633255E-5 1.4618517505746587 7.022752401013208E-4 88-89 0.0 0.0 6.583830375949883E-5 1.726894815409148 7.24221341354487E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12115 0.0 28.407215 1 GGTATCA 8565 0.0 26.69508 1 GTACATG 8400 0.0 25.069056 1 ATCAACG 14270 0.0 23.912144 3 TCAACGC 14330 0.0 23.812023 4 CAACGCA 14455 0.0 23.635347 5 TATCAAC 14570 0.0 23.457561 2 TACATGG 8685 0.0 23.261229 2 AACGCAG 14930 0.0 22.978361 6 GAGTACT 7455 0.0 22.117348 12-13 ACATGGG 8795 0.0 21.722422 3 AGAGTAC 12115 0.0 20.807863 10-11 ACGCAGA 16360 0.0 20.708466 7 CGCAGAG 16325 0.0 20.522268 8 AGTACTT 7975 0.0 20.496464 12-13 GTACTTT 8145 0.0 19.951773 14-15 GCAGAGT 17130 0.0 18.920626 9 CATGGGG 4120 0.0 17.648682 4 ACGTTAT 135 0.009378771 17.600527 8 GTGTAGC 1270 0.0 17.591694 1 >>END_MODULE