FastQCFastQC Report
Thu 26 May 2016
SRR936871_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936871_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2278309
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92620.40652957961365205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73550.32282714943407587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48620.21340388858578885No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39200.1720574338248236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA134100.027.4200611
GGTATCA95100.026.1762221
GTACATG85250.023.7952181
ATCAACG157200.023.1142233
TCAACGC157650.023.0477434
CAACGCA159150.022.800675
TATCAAC161100.022.7915292
TACATGG86300.022.5113752
AACGCAG162550.022.3529746
ACATGGG84750.021.9132843
GAGTACT86550.021.5942712-13
CTATACG1150.0037260120.6506184
AGAGTAC131600.020.55411310-11
AGTACTT92000.020.4441112-13
ACGCAGA178950.020.1451727
GTACTTT92050.019.96905914-15
CGCAGAG181150.019.821868
CATGGGG39750.019.1180574
GCAGAGT188000.018.6448739
GTGTAGG12700.017.2072051
Produced by FastQC (version 0.11.5)