FastQCFastQC Report
Thu 26 May 2016
SRR936870_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936870_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2097079
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78660.3750931653027854No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57120.27237886603222866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37290.17781876600738455No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33600.1602228623718992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA119750.030.16131
TCAACGC137300.025.9586434
ATCAACG137750.025.9083393
CAACGCA137800.025.8669225
GGTATCA82450.025.5920051
TATCAAC140900.025.465252
AACGCAG140400.025.4531766
GTACATG77000.023.268191
GAGTACT71650.023.0124612-13
ACGCAGA155400.022.782797
GACGCGT1050.002190714222.6298858
CGCAGAG157000.022.3689928
TACATGG77300.021.8868122
GTACTTT76450.021.31770914-15
ACATGGG75600.021.246443
TAACGCA1353.7857328E-421.120724
AGTACTT75100.020.68986112-13
AGAGTAC112900.020.64651110-11
GCAGAGT164900.020.4904069
ACGCGTG1600.001187638117.8210329
Produced by FastQC (version 0.11.5)